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Germplasm Resources, Fruit Cultivation and Biotechnology Application in Fruit Breeding
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Germplasm Resources, Fruit Cultivation and Biotechnology Application in Fruit Breeding

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (30 April 2025) | Viewed by 832

Special Issue Editors

Dr. Yaqiong Wu

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Guest Editor
Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Interests: biotechnology; small berry; fruit; genetic breeding; function
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Weilin Li

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Guest Editor
College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Interests: blueberry; blackberry; germplasm resources; breeding; efficient cultivation; fruit processing
Special Issues, Collections and Topics in MDPI journals
Dr. Haiyan Yang

E-Mail Website
Guest Editor
Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China
Interests: blueberry; blackberry; biotechnology; cultivation technique; fruit quality control

Special Issue Information

Dear Colleagues,

Excellent cultivars and efficient cultivation techniques are the foundation of high-quality and yielding fruit trees. In recent years, breeding technologies such as transgenics, gene editing, and molecular markers have developed rapidly, significantly improving breeding efficiency and variety improvement. The cultivation mode of fruit trees also tends to be diversified, transitioning from the traditional open field cultivation mode to the coexistence of rain protection cultivation, facilities to promote early cultivation, soilless cultivation, and other modes. This Special Issue focuses on the collection, preservation, evaluation, and utilization of fruit tree germplasm resources, technical research on pruning, and environmental control such as temperature, light, water, fertilizer, etc. It also focuses on the pest and disease control of fruit trees under different cultivation modes, the regulatory mechanism of fruit quality formation, and the application of biotechnology in the research of fruit breeding, outlining the latest research results of fruit tree workers in these fields, which will serve as technical references for the cultivation of new fruit tree varieties, promote academic exchanges, and facilitate the sustainable development of fruit tree improvement work.

Dr. Yaqiong Wu
Prof. Dr. Weilin Li
Dr. Haiyan Yang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fruit tree
  • germplasm resources
  • cultivation techniques
  • biotechnology
  • growth and development
  • fruit quality
  • regulation mechanisms, selection and breeding

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Published Papers (2 papers)

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Research

19 pages, 7427 KiB  
Article
Analysis of Meiotic Behavior and 2n Pollen Formation Frequency in Triploid Hybrids of Chinese Jujube
by Yunxi Zhong, Lixin Ge, Yinfang Song, Zhi Luo, Jiurui Wang, Mengjun Liu and Fenfen Yan
Plants 2025, 14(11), 1643; https://doi.org/10.3390/plants14111643 - 28 May 2025
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Abstract
The Chinese jujube (Ziziphus jujuba Mill.), an economically significant fruit tree native to China, is valued for both fresh and dried uses. In plants, 2n gametes serve as the fundamental basis for creating a sexual polyploid germplasm. This study investigated the 2n [...] Read more.
The Chinese jujube (Ziziphus jujuba Mill.), an economically significant fruit tree native to China, is valued for both fresh and dried uses. In plants, 2n gametes serve as the fundamental basis for creating a sexual polyploid germplasm. This study investigated the 2n gametogenesis frequency in triploid hybrid jujubes through meiotic analysis of the hybrid strain Q161 and a two-year pollen analysis on hybrid progeny, assessing the natural 2n pollen frequencies to identify a high-2n-pollen germplasm and revealing the occurrence of 2n pollen. Meiotic analysis of the triploid hybrid Q161 (2n = 36) revealed cytological anomalies, including binucleate cells (22.80% abnormal tetrads), with natural 2n pollen production rates reaching 4.00% and 4.67% over two consecutive years. Scanning electron microscopy (SEM) revealed that the 2n pollen exhibited pronounced exine ornamentation with cerebroid sculpturing and tubercle-like structures at the apertures. Analysis of the triploid progeny for two consecutive years demonstrated a pollen viability of 30.45% and 23.83% (CV: 19. 39–29.69%), with the mean 2n pollen frequencies of 22.52% and 7.64%, peaking at 52.16% and 28.95% in elite individuals. Six triploid germplasm accessions with naturally elevated 2n pollen frequencies were identified. Under natural conditions, a triploid hybrid germplasm in Chinese jujube produces 2n pollen grains due to abnormal meiotic behavior, and a natural triploid germplasm with high pollen productivity was identified. This research provides a critical theoretical foundation for sexual polyploid breeding strategies. Full article
(This article belongs to the Special Issue Germplasm Resources, Fruit Cultivation and Biotechnology Application in Fruit Breeding)
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20 pages, 13027 KiB  
Article
Growth and Physiological Traits of Blueberry Seedlings in Response to Different Nitrogen Forms
by Haiyan Yang, Yaqiong Wu, Chunhong Zhang, Lianfei Lyu, Wenlong Wu, Zhengjin Huang and Weilin Li
Plants 2025, 14(10), 1444; https://doi.org/10.3390/plants14101444 - 12 May 2025
Viewed by 255
Abstract
This study aimed to better understand the impacts of various nitrogen (N) forms on blueberry growth and development, as well as to increase blueberry (Vaccinium spp.) N utilization efficiency. We selected the blueberry cultivar ‘Anna’ as the experimental material, and four N [...] Read more.
This study aimed to better understand the impacts of various nitrogen (N) forms on blueberry growth and development, as well as to increase blueberry (Vaccinium spp.) N utilization efficiency. We selected the blueberry cultivar ‘Anna’ as the experimental material, and four N treatments were applied throughout the key vegetative growth stage: N deficiency (CK), ammonium-N (T1), nitrate-N (T2), and amide-N (T3). The growth parameters, physiology indexes, and ultrastructure changes in blueberry seedlings were explored. At the same time, the Pearson correlation model was used to analyze the correlation among each physiology index. The results showed that blueberry plants grew better under T1 and T3 treatments, with increased biomass, N content, chlorophyll content, and photosynthetic efficiency. Under T1 treatment, the leaves had lower O2˙− generation rate and MDA concentration, but higher superoxide dismutase (SOD), glutamate synthetase (GOGAT), and glutamine synthetase (GS) activity. Compared to T1 treatment, T2 treatment dramatically enhanced peroxidase (POD) activity, glucose content, and free amino acid content, particularly Arg content. Furthermore, N deficit treatment inhibited plant growth while increasing free radicals, POD, catalase (CAT), and glutamate dehydrogenase (GDH) activities, as well as the content of antioxidant compounds. Correlation and principal component analysis showed that photosynthetic properties, chlorophyll content, antioxidant system, amino acid levels, and N metabolizing enzyme activity were significantly affected by different N forms. This study can serve as a scientific foundation for optimal N regulation and management in blueberries. Full article
(This article belongs to the Special Issue Germplasm Resources, Fruit Cultivation and Biotechnology Application in Fruit Breeding)
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