Search Results (188)

To develop carbon farming practices, decision-makers need detailed spatial data on the soil carbon sequestration (SCS) opportunities that conventional crop and soil management creates. This study exploratorily assessed SCS capacity across agricultural land in the Valencian Community using a simple carbon balance model within a GIS framework. Within this modelling approach, maps of net primary production (NPP), land-use-derived crop harvest indices, current soil organic carbon (SOC) stocks, and NPP and SOC mineralization coefficients were combined. Results show that while NPP across Valencian croplands and grasslands ranges from 0.64 to 6.43 Mg C ha⁻¹ yr⁻¹ (mean 2.42 Mg C ha⁻¹ yr⁻¹), the actual SCS capacity is much lower, ranging from −0.04 to 1.31 Mg C ha⁻¹ yr⁻¹ (mean 0.25 Mg C ha⁻¹ yr⁻¹). Significant variation exists among land uses: rice paddies exhibit the highest SCS capacity, while olive groves present the lowest. Between 2017 and 2021, SCS in Valencian agroecosystems may have offset the sector’s greenhouse gas (GHG) emissions, primarily driven by pasture and citrus because of their large extent and moderate SCS capacity, making agriculture a net-zero emitter. However, helping achieve cross-sectoral mitigation targets will depend in part on the widespread deployment of regenerative soil management (RSM) practices. While this study identifies priority areas for RSM implementation, further research is needed to determine which specific practices are most suitable for each location to maximize SCS. Full article

The use of new grape cultivars is essential for the continued development of Brazilian viticulture. Thus, this study aimed to determine the general physicochemical parameters, global phenolic composition, and antioxidant capacity of grape musts from the Portuguese red variety ‘Touriga Nacional’ during ripening in two Brazilian vineyards (states of Rio Grande do Sul and Santa Catarina). The results were compared with data obtained from the same variety cultivated in a vineyard located in northern Portugal, which is the region of origin of this variety. This research was conducted over three consecutive vintages (2022–2024). Overall, the results indicated that soil and climate conditions at each location were associated with differences in the composition of ‘Touriga Nacional’ grape musts. Grapes from both Brazilian vineyards showed significantly higher berry weight, must volume, and yield compared with grapes collected from the Portuguese vineyard. On the other hand, grapes collected from the vineyard located in the state of Santa Catarina exhibited the highest values of total titratable acidity, malic acid, total phenols, flavonoids, total anthocyanins, and chromatic characteristics. Nevertheless, grapes collected from the Portuguese vineyard showed higher values of total tannins. The results suggest that the ‘Touriga Nacional’ variety shows better adaptation to the conditions of the Santa Catarina vineyard than to those of the Rio Grande do Sul vineyard. These findings help winegrowers, particularly in southern Brazil, to have more options for introducing different grape varieties, thereby contributing to the production of wines with distinctive characteristics, while consumers will have access to a greater diversity of wines available on the market. Full article

This study focuses on the Helan Mountain East Foothills region of Ningxia, a typical continental climate wine-growing area, with Cabernet Sauvignon grapes as the subject. It combines trimethylsilyl derivatization–Gas Chromatography–Mass Spectrometry (TMS-GC-MS) technology and the independently developed AntDAS-GCMS data analysis platform. The aim was to systematically characterize the temporal dynamics of primary and secondary polar metabolites throughout the entire growth cycle of Cabernet Sauvignon in this region. Results identified 50 metabolites exhibiting significant differences (fold change ≥1, p < 0.05) across growth stages, primarily comprising organic acids (18), sugars (7), and amino acids (13). Metabolite accumulation demonstrated distinct stage-specific patterns: organic acids (e.g., tartaric acid, malic acid) peaked before veraison and then declined significantly, while sugars (e.g., fructose) exhibited a marked increase in abundance during the late maturation stage. The underlying mechanisms of the relevant metabolic pathways require further validation through multi-omics approaches. This study elucidates the dynamic characteristics of primary and secondary metabolites throughout the entire growth stages of Cabernet Sauvignon in the region of Ningxia. It provides data support for understanding the metabolic basis of flavor development in grapes from this area and offers practical references for quality regulation and harvest timing optimization in local grape cultivation management. Full article

This study focuses on identifying wine-growing subzones within the PDO Amyndeon and PGI Drama wine-growing zones in Northern Greece, with the aim of assessing their suitability for producing high-quality red wines from the Xinomavro (Vitis vinifera L.) and Cabernet Sauvignon (Vitis vinifera L.) grape varieties, respectively. The initial delineation of suitability zones was carried out using readily available satellite data on soil, topography, and climate, in four different suitability categories. To validate how effectively these categories distinguished actual wine-growing regions, we compared them against two years of field data collected from experimental vineyards. The results showed that this methodology was able to discern the most suitable areas for both varieties and regions with an acceptable relation to real grape and wine attributes as confirmed by the collection of data from the pilot vineyards. The overall performance of this method will ultimately depend on the validity of the expert knowledge used to define the most critical parameters and their range. According to the results of this study, and given the relevance of the proposed suitability criteria, this method has the potential to provide an alternative solution for subzone delineation in cases where wine analytical and sensory data are not available. Full article

This study investigates the effects of terroir on the sensory expression of two major white grape varieties, Fetească albă and Sauvignon blanc. The experimental design included wines from eleven wineries distributed across the main Romanian wine-growing regions—Banat, Dobrogea, Moldova, Muntenia–Oltenia, and Transylvania—covering a wide range of altitudes (75–400 m), latitudes (21.6–28.4°), and contrasting soil types (chernozems, alluvial soils, luvaceous and clay-illuvial brown soils) over two climatically distinct vintages (2019 and 2021). Volatile profiling was performed by GC–MS, and aroma relevance was assessed using odor activity values (OAVs) and weighted aromatic scores, while sensory attributes were integrated through structured sensory evaluation. Esters and thiols emerged as the dominant contributors to varietal aroma expression. Sensory aggregation revealed clear winery-dependent differences, whereas vintage effects were moderate, with 2021 wines displaying a more pronounced fruity–floral profile compared to 2019. Linear Discriminant Analysis (LDA) identified grape variety as the strongest discriminant factor, surpassing vintage, and confirmed distinct regional sensory identities. The integrated OAV–sensory–LDA framework demonstrates the defining role of terroir in shaping aromatic structure, enabling robust varietal typicity assessment and regional differentiation across Romanian wine-growing areas. Full article

Grapevine downy mildew is a major disease in vineyards all around the world, caused by the oomycete Plasmopara viticola (Berk. & M. A. Curtis) Berl. & De Toni. Normally, its control depends almost exclusively on chemical and copper-based fungicides, especially in high-incidence areas with high relative humidity and mild temperatures. However, the European Union is determined to reduce the application of these phytochemicals by at least 50% by 2030, forcing winegrowers to seek alternative low-input strategies for proper sanitary maintenance. Basic substances (BSs), described in European Regulation (EC) 1107/2009, stand out as promising alternatives, but their molecular mechanism of action remains mostly unknown. In this context, this study analyzed the genetic effect in grapevine plants of several commercial products composed of BSs (chitosan, soy lecithin, Equisetum arvense and Salix cortex). All products exhibited promising results, triggering the induction of similar defence mechanisms, which included pathogenesis-related proteins (PRs), involved in direct pathogen repression; stilbenes, capable of producing antimicrobial compounds such as resveratrol and pterostilbene; several hormones, including oxylipins, ethylene, salicylic acid and terpenes, mediating immune signalling; and genes related to structural features of the plant, such as lignin, callose, cellulose and cuticular wax, constituting a first physiological barrier against P. viticola. Disease severity reduction differed among treatments, with Salix cortex showing the highest efficacy (58%), followed by BABA (38%) and LESOY (35%), while LECI and CHIT had minor effects (<9%). Gene expression analyses revealed that Salix cortex modulated the highest percentage of genes (41%), followed by natural infection without treatment (32%), LESOY (27%), BABA (26%), LECI (23%) and CHIT (23%). In terms of defence mechanisms, Salix cortex promoted the most pathways, LESOY induced eight, BABA and LECI seven and CHIT five. Overall, these results indicate that BSs can modulate several defence pathways in grapevine, supporting their potential use as sustainable alternatives for controlling downy mildew. Full article

Globalization, climate change and the energy crisis are having a negative impact on the agricultural sector. Moreover, in Greece, producers need to invest in modernizing their farms, introducing innovations, achieve digital transformation and increase productivity. Incentives are available for green investments, thus facilitating the adoption of innovations. This paper examines the attitudes and perceptions of winegrowers in Western Macedonia regarding environmental protection and organic farming. Specifically, with the help of the k-means algorithm, respondents are divided into two groups. The first group consists of producers who have a negative attitude towards environmental issues and have a lower level of education, while the second group consists of those who have a positive attitude. In the first group in particular, most respondents do not seem to have a good knowledge of environmental protection issues, believe that cross-compliance is very stringent and do not have a high level of education. This means that policy measures must be taken to adequately inform and educate farmers belonging to this group to achieve the goal of environmental protection. The results of the second group include producers with a positive attitude, who are expected to play an important role in the development of sustainable viticulture by creating conditions for a better future at an individual, regional and social level. Full article

Bioclimatic monitoring at vineyard scale is essential for irrigation management and disease-risk assessment, yet many systems rely on expensive commercial stations or generic IoT nodes with limited validation and little focus on small and medium-sized winegrowers. This application-driven engineering work investigates whether decision-support-grade bioclimatic data for precision viticulture can be obtained from a low-cost station, by proposing a solar-powered proximal node that integrates soil, plant, and atmospheric sensors on a dedicated PCB that communicates via LoRaWAN. The node operates in a 15-min cycle, with sensing parameters selected to provide the minimum information required for key Precision Viticulture applications. It was deployed in a commercial vineyard side by side with a commercial station, quantifying sensor agreement, communication reliability, and energy consumption. The results show low error rates and consistent agronomic interpretation of environmental conditions, disease risk, precipitation events, and soil and water dynamics. The LoRaWAN link reached a 97% packet-delivery ratio with an average consumption of about 2.5 Wh per day. Material cost is approximately 260 €, one order of magnitude lower than a comparable station. These results indicate that, under real vineyard conditions and compared with a commercial reference, the proposed low-cost system provides agronomically useful, reliable bioclimatic monitoring. Full article

Gray mold, caused by Botrytis cinerea Pers. Fr. (teleomorph: Botryotinia fuckeliana), is a major disease affecting Moroccan vineyards. However, limited information is available on the natural populations of this pathogen. In this study, 82 single-spore isolates collected from vineyards in two major wine-growing regions were evaluated for phenotypic, physiological, and molecular variability. The isolates exhibited differences in morphotypes, conidial size, and sclerotia production on PDA medium. Temperature significantly affected mycelial growth rate (mm d⁻¹). All isolates were virulent on grapevine leaves, showing varying levels of aggressiveness. Among the representative isolates, 20 were heterothallic and 2 were homothallic. Mating-type analysis revealed that 12% belonged to MAT1-1 and 75% to MAT1-2. Transposable element genotyping showed that the population was composed of 41.7% transposa, 29.2% vacuma, 16.7% Flipper-only, and 12.5% Boty-only. This work represents the first report on genotypic variation in B. cinerea populations from Moroccan vineyards. The findings provide new insights into the morphenotypic and genetic diversity of the pathogen and may support the development of improved strategies for disease management. Full article

This study characterised the diversity of filamentous fungi and yeasts during Malbec grape fermentation in the Southern Oasis of Mendoza (Argentina) winegrowing region, under different sanitary conditions and SO₂ treatments, using morphological and ITS-RFLP-based molecular methods. Alternaria, Cladosporium and Penicillium were present in both sound and damaged grapes, while Aspergillus and Botrytis were primarily found in damaged grapes. The predominant yeast species in both sound and damaged grape must, at lower and higher maturity levels, were Aureobasidium pullulans and Hanseniaspora spp. At higher grape ripening levels species diversity increased, with Hanseniaspora vineae, Metschnikowia pulcherrima and Candida membranifaciens dominating, and others such as Pichia kudriavzevii and Issatchenkia terricola appearing. A. pullulans and M. pulcherrima were highly tolerant to SO₂. Notably, the species Meyerozyma guilliermondii, Zygoascus hellenicus and Hanseniaspora uvarum were exclusively present in damaged grape must, while Zygosaccharomyces bailii was also found in sound grape must. Hanseniaspora spp. and P. kudriavzevii predominated at mid-fermentation and persisted at the end of the process, highlighting their resistance to wine conditions and their potential to influence post-fermentative dynamics. These findings emphasise the significant influence of grape sanitary status on mycobiota composition, with important implications for fermentation behaviour and final wine quality. Full article

A study was carried out to identify the filamentous fungi and yeasts present in rotten wine grapes from two subzones of the Southern oasis of Mendoza winegrowing region, to assess the occurrence of tenuazonic acid (TA), a mycotoxin produced by the Alternaria genus, and to evaluate the wine spoilage potential of the associated yeasts in vitro and during microvinifications. The main fungal genera present were Alternaria (69.3%), followed by Aspergillus (16.8%), Penicillium (9.3%), and Cladosporium (4.6%), while the dominant yeast species Metschnikowia pulcherrima (23.1%), Aureobasidium pullulans (20.2%) and Hanseniaspora uvarum (13.0%) were followed by H. vineae (11.6%), Zygosaccharomyces bailii (10.4%), and H. guilliermondii (9.2%). Additionally, 94.1% of the rotten samples were contaminated with TA, with the highest level found in the Cabernet Sauvignon variety. No geographic association was found in the incidence of the different fungal genera or yeast species, nor in the occurrence of TA. Almost all of the tested yeasts produced H₂S, the majority of the Hanseniaspora strains produced acetic acid, and only one M. pulcherrima strain produced off-flavours in in vitro tests. Wines co-fermented with H. uvarum L144 and S. cerevisiae showed higher volatile acidity and lower fruity aroma and taste intensity. Therefore, processing bunch rot could pose a toxicological and microbiological risk to winemaking due to the high incidence of Alternaria and TA, as well as the potential of the associated yeasts to spoil wine. Full article

At a time marked by a transition in winegrowing methods, the decision to employ biodynamic viticulture, and its holistic approach, is a choice made by many technicians in the French wine industry. Nevertheless, this practice is perceived very differently among industry members. Some frequently debate its foundations and tangible benefits, while others question the value of scientific research conducted on its methods. The representations motivating the decision to pursue biodynamic practices remain unclear. Thirty members of the industry were interviewed about their opinions and knowledge on biodynamics through semi-structured interviews. A textual analysis of their responses, using Alceste software (Image Ltd., Toulouse, France, version 2018), revealed four key representations of this growing method within the industry. Some practitioners believe that biodynamic preparations may have a material effect on plant behaviour and that the effects of biodynamic viticulture on the final quality of wine should be studied more closely. Others suggest that the anthroposophical foundations of the method should be set aside to allow for its reinvention in a forward-looking manner. Lastly, experimental methods and peer-exchanges are considered essential to understanding the effects of this cultivation method in specific contexts and terroirs. These different viewpoints should be integrated to develop innovative and interesting applications for biodynamic farming methods. Full article

Ligurian viticulture is characterized by a fragmented landscape and diverse microclimatic conditions, presenting both challenges and opportunities for grape production under climate change. This study investigates the perceived impacts of climate change on viticulture in Eastern (Levante) and Western (Ponente) Liguria, with a focus on vine growth dynamics, productivity, and the adaptation strategies adopted by local winegrowers. Semi-structured interviews with 48 winemakers revealed significant shifts in grape maturation (p < 0.001), earlier harvest dates, and increased vulnerability to fungal diseases (p < 0.01), primarily driven by rising temperatures and altered precipitation regimes. A notable rise in extreme temperature events (p < 0.01) was reported, with all respondents (100%) observing irregular seasonal temperature fluctuations. Furthermore, climate change was linked to changes in local fauna, particularly the expansion of ungulate populations (p < 0.001), leading to increased vineyard damage. In response, growers have adopted a range of adaptive measures, including drought-resistant rootstocks (e.g., M-series), traditional training systems (Guyot, Alberello), and local innovations such as the low pergola in Cinque Terre. Principal component analysis (PCA) revealed region-specific adaptation profiles, underscoring the influence of environmental and agronomic variability on viticultural resilience. Logistic regression identified temperature variability, disease incidence, and precipitation shifts as key predictors of perceived climate impact. The results underscore the urgency of developing regionally tailored adaptation strategies to sustain viticulture in Liguria’s complex and changing landscape. Full article

Climate change is having a significant impact on vine physiology and grape composition, leading to notable alterations in wine quality, such as reduced acidity, increased ethanol content, and higher pH levels. These effects are particularly problematic in arid and semi-arid regions, such as Mediterranean areas, where high summer temperatures and low rainfall accelerate the degradation of organic acids in grapes. As a result, wines produced under these conditions often lack the acidity required to preserve their freshness and enological quality. This study evaluated the effect of must acidification using cation-exchange resins on the composition and quality of red wines made from the Monastrell variety, comparing them with wines acidified using tartaric acid to reach the same target pH. The results showed that treating a portion of the must (20% and 30%) with cation-exchange resins significantly reduced wine pH values and increased total acidity compared to the control wine. A similar result was observed in wines acidified with tartaric acid. However, as an additional effect, the treatment with resin more markedly reduced the concentration of pro-oxidant metal cations such as iron, copper, and manganese, contributing to lower values of volatile acidity and a greater stability against oxidation of phenolic compounds. Must acidification with both methods improved wine color quality by increasing color intensity and decreasing hue values. Although no significant differences were found in the total concentration of phenolic compounds, variations were detected in their compositional profile. Furthermore, the acidification also affected the concentration and composition of aromatic compounds in the final wine. Sensory analysis revealed that the treated wines—particularly those made with must acidified using cation-exchange resins—exhibited greater aromatic intensity, more pronounced fruity notes, and reduced astringency, resulting in a fresher mouthfeel. In conclusion, must treatment with cation-exchange resins appears to be a low-cost good alternative compared tartaric acid addition for reducing pH and increasing acidity in Monastrell red wines, thereby enhancing their quality in winegrowing regions with arid or semi-arid climates. Full article

Due to climate change, the risk of spring frosts has increased and may rise further in the near future. This is pushing winegrowers to adopt active spring frost protection methods (ASFPMs) in their vineyard management practices. This study analyzes the potential contribution of the most commonly used ASFPMs to the environmental impacts of grape production in the Loire Valley region, using the Life Cycle Assessment (LCA) approach, while considering local mesoclimatic conditions. The environmental offsets of ASFPMs are modeled by comparing the viticulture stage impact with and without ASFPM technologies. Furthermore, the present paper proposes an original approach to integrate potential yield loss, simulating frost damage. This sensitivity analysis identifies the yield loss threshold at which the different ASFPMs are environmentally compensated under various mesoclimatic conditions. We show that the environmental contribution of instant ASFPMs varies most significantly based on the number of frost hours, but generally remains the highest across most environmental indicators compared to other impacts of viticulture, e.g., ranging from 35 to 92% for the climate change indicator. Wind machines contribute the least to the viticulture stage, regardless of frost hour occurrence. However, even permanent solutions have a significant impact on at least one environmental indicator, regardless of frost hour occurrence. Additionally, the environmental offset analysis outlines that the yield loss thresholds for ASFPM impact compensation are high, even for the most effective solutions in a frost-prone context. Future research should include passive spring frost protection methods and other types of vineyard management in LCA of the viticulture stage. Full article