Search Results (495)

Background: Accumulating evidence indicates that SARS-CoV-2 infection results in long-term multiorgan complications, with the kidney being a primary target. This study aimed to characterize the long-term transcriptomic changes in the kidney following coronavirus infection using a murine model of MHV-1-induced SARS-like illness and to evaluate the therapeutic efficacy of SPIKENET (SPK). Methods: A/J mice were infected with MHV-1. Renal tissues were collected and subjected to immunofluorescence analysis and Next Generation RNA Sequencing to identify differentially expressed genes associated with acute and chronic infection. Bioinformatic analyses, including PCA, volcano plots, and GO/KEGG pathway enrichment, were performed. A separate cohort received SPK treatment, and comparative transcriptomic profiling was conducted. Gene expression profile was further confirmed using real-time PCR. Results: Acute infection showed the upregulation of genes involved in inflammation and fibrosis. Long-term MHV-1 infection led to the sustained upregulation of genes involved in muscle regeneration, cytoskeletal remodeling, and fibrotic responses. Notably, both expression and variability of SLC22 and SLC22A8, key proximal tubule transporters, were reduced, suggesting a loss of segment-specific identity. Further, SLC12A1, a critical regulator of sodium reabsorption and blood pressure, was downregulated and is associated with the onset of polyuria and hydronephrosis. SLC transporters exhibited expression patterns consistent with tubular dysfunction and inflammation. These findings suggest aberrant activation of myogenic pathways and structural proteins in renal tissues, consistent with a pro-fibrotic phenotype. In contrast, SPK treatment reversed the expression of most genes, thereby restoring the gene profiles to those observed in control mice. Conclusions: MHV-1-induced long COVID is associated with persistent transcriptional reprogramming in the kidney, indicative of chronic inflammation, cytoskeletal dysregulation, and fibrogenesis. SPK demonstrates robust therapeutic potential by normalizing these molecular signatures and preventing long-term renal damage. These findings underscore the relevance of the MHV-1 model and support further investigation of SPK as a candidate therapy for COVID-19-associated renal sequelae. Full article

The advanced determination of the type (foreshock–aftershock–swarm) of an ongoing seismic cluster is quite challenging; only retrospective solutions have thus far been proposed. In the period of January–March 2025, a seismic cluster, recorded between Santorini volcano and Amorgos Island, South Aegean Sea, caused considerable social concern. A rapid increase in both the seismicity rate and the earthquake magnitudes was noted until the mainshock of M_L = 5.3 on 10 February; afterwards, activity gradually diminished. Fault-plane solutions indicated SW-NE normal faulting. The epicenters moved with a mean velocity of ~0.72 km/day from SW to NE up to the mainshock area at a distance of ~25 km. Crucial questions publicly emerged during the cluster. Was it a foreshock–aftershock activity or a swarm of possibly volcanic origin? We performed real-time discrimination of the cluster type based on a daily re-evaluation of the space–time–magnitude changes and their significance relative to background seismicity using earthquake statistics and the topological metric betweenness centrality. Our findings were periodically documented during the ongoing cluster starting from the fourth cluster day (2 February 2025), at which point we determined that it was a foreshock and not a case of seismic swarm. The third day after the M_L = 5.3 mainshock, a typical aftershock decay was detected. The observed foreshock properties favored a cascade mechanism, likely facilitated by non-volcanic material softening and the likely subdiffusion processes in a dense fault network. This mechanism was possibly combined with an aseismic nucleation process if transient geodetic deformation was present. No significant aftershock expansion towards the NE was noted, possibly due to the presence of a geometrical fault barrier east of the Anydros Ridge. The 2025 activity offered an excellent opportunity to investigate deciphering the type of ongoing seismicity cluster for real-time discrimination between foreshocks, aftershocks, and swarms. Full article

Inspired by the active site of methane monooxygenase, we designed a Cu₂O cluster anchored in the six-membered nitrogen cavity of a C₂N monolayer (Cu₂O@C₂N) as a stable and efficient enzyme-like catalyst. Density functional theory (DFT) calculations reveal that the bridged Cu-O-Cu structure within C₂N exhibits strong electronic coupling, which is favorable for methanol formation. Two competing mechanisms—the concerted and radical-rebound pathways—were systematically investigated, with the former being energetically preferred due to lower energy barriers and more stable intermediate states. Furthermore, strain engineering was employed to tune the geometric and electronic structure of the Cu-O-Cu site. Biaxial strain modulates the Cu-O-Cu bond angle, adsorption properties, and d-band center alignment, thereby selectively enhancing the concerted pathway. A volcano-like trend was observed between the applied strain and the methanol formation barrier, with 1% tensile strain yielding the overall energy barrier to methanol formation (ΔG_overall) as low as 1.31 eV. N₂O effectively regenerated the active site and demonstrated strain-responsive kinetics. The electronic descriptor Δε (ε_d − ε_p) captured the structure–activity relationship, confirming the role of strain in regulating catalytic performance. This work highlights the synergy between geometric confinement and mechanical modulation, offering a rational design strategy for advanced C1 activation catalysts. Full article

Volcanic eruptions release gases and particulates that may adversely affect human health. The Tajogaite eruption on La Palma provided a unique opportunity to evaluate inorganic pollutant exposure in a directly affected population. As part of the ISVOLCAN study, blood samples from 393 adults residing in the island’s western region were analyzed for 43 inorganic elements using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), including 20 toxic elements identified by the Agency for Toxic Substances and Disease Registry (ATSDR). The median age of participants was 51 years, and 56.7% were female. Higher levels of Hg and Mn were associated with long-term occupational exposure, while smoking was linked to elevated Cd, Pb, and Sr levels. Participants living within 6.5 km of the volcano had significantly higher concentrations of Al and Ti. Ash cleanup activities were associated with increased levels of Ni and Cu, and those spending over five hours outdoors daily showed elevated Se and Pb. This is the first biomonitoring study to assess blood concentrations of inorganic pollutants in a population exposed to volcanic emissions. The findings highlight key exposure factors and underscore the need for continued research to assess long-term health effects and inform public health measures. Full article

The South Shetland Islands and Antarctic Peninsula (SHETPENANT region) constitute a geodynamically active area shaped by the interaction of major tectonic plates and active magmatic systems. This study analyzes GNSS time series spanning from 2017 to 2024 to investigate surface deformation associated with the 2020–2021 seismic swarm near the Orca submarine volcano. Horizontal and vertical displacement velocities were estimated for the preseismic, coseismic, and postseismic phases using the CATS method. Results reveal significant coseismic displacements exceeding 20 mm in the horizontal components near Orca, associated with rapid magmatic pressure release and dike intrusion. Postseismic velocities indicate continued, though slower, deformation attributed to crustal relaxation. Stations located near the Orca exhibit nonlinear, transient behavior, whereas more distant stations display stable, linear trends, highlighting the spatial heterogeneity of crustal deformation. Stress and strain fields derived from the velocity models identify zones of extensional dilatation in the central Bransfield Basin and localized compression near magmatic intrusions. Maximum strain rates during the coseismic phase exceeded 200 $\mathrm{\nu }$strain/year, supporting a scenario of crustal thinning and fault reactivation. These patterns align with the known structural framework of the region. The integration of GNSS-based displacement and strain modeling proves essential for resolving active volcano-tectonic interactions. The findings enhance our understanding of back-arc deformation processes in polar regions and support the development of more effective geohazard monitoring strategies. Full article

Propylene production through the CO₂-assisted oxidative dehydrogenation of propane (CO₂-ODP) is an effective route able to address the ever-increasing demand for propylene and simultaneously utilize CO₂. In this study, a series of alumina-supported gallium oxide catalysts of variable Ga₂O₃ loading was synthesized, characterized, and evaluated with respect to their activity for the CO₂-ODP reaction. It was found that both the catalysts’ physicochemical characteristics and performance were strongly affected by the amount of Ga₂O₃ dispersed on Al₂O₃. Surface basicity was maximized for the sample containing 20 wt.% Ga₂O₃, whereas surface acidity was monotonically increased with increasing Ga₂O₃ loading. A volcano-type correlation was found between catalytic performance and acid/base properties, according to which propane conversion and propylene yield exhibited optimum values for intermediate surface basicity and acidity, which both correspond to the sample containing 30 wt.% Ga₂O₃. The dispersion of a suitable amount of Ga₂O₃ on the Al₂O₃ surface not only enhances the conversion of propane to propylene but also suppresses the formation of side products (C₂H₄, CH₄, and C₂H₆) at temperatures of practical interest. The 30%Ga₂O₃-Al₂O₃ catalyst exhibited very good stability at 550 °C, where byproduct formation and carbon deposition were limited. Mechanistic studies indicated that the reaction proceeds through a two-step oxidative route with the participation of CO₂ in the abstraction of H₂, originating from propane dehydrogenation, through the reverse water–gas reaction (RWGS) reaction, shifting the thermodynamic equilibrium towards propylene generation. Full article

The need to promote proximity tourism in natural areas has made the destinations in the interior of the Iberian Peninsula a benchmark in the development of tourism in traditionally rural and depressed areas. In Ciudad Real province, a sustainable territorial management project was developed based on the uniqueness of its volcanic geoheritage, leading to its designation as the UNESCO Global Geopark “Volcanes de Calatrava, Ciudad Real”. Geoparks promote sustainable activities such as geotourism; thus, an urban geotourism itinerary was proposed in Piedrabuena (Ciudad Real, Castilla-La Mancha) in order to make the natural and cultural geoheritage of a part of the geopark known, which is also a part of the Campo de Calatrava Volcanic Region. For this purpose, 12 natural and cultural resources of the geoheritage of the Piedrabuena volcano, on which the urban centre is located, were identified, inventoried, characterized, and valorised as sites of geotouristic interest, and they were connected along a geotourism itinerary. This itinerary has a 4 km route and up to eight interpretive stops, which allows visitors and tourists to enjoy a geotourism experience based on the volcanic landscape of the geopark, laying the foundations for the creation of other similar experiences. Full article

Geothermal energy is a low-carbon and reliable energy resource capable of generating both heat and electricity from the Earth’s internal thermal energy. While geothermal development has traditionally been focused on onshore sites, offshore geothermal resources are attracting growing interest due to advancements in technology, the search for alternative baseload power, and the opportunity to repurpose decommissioned petroleum infrastructure. Recent efforts include utilizing abandoned oil and gas fields to adapt existing infrastructure for geothermal use, as well as exploring high-temperature geothermal zones such as submarine volcanoes and hotspots. Despite these initiatives, research output, scientific publications and patents remain relatively limited, suggesting that offshore geothermal technology is still in its early stages. Countries like Italy, Indonesia and Turkey are actively investigating geothermal resources in volcanic marine areas, while North Sea countries and the USA are assessing the feasibility of converting mature oil and gas fields into geothermal energy sites. These diverse strategies underscore the regional geological and infrastructure conditions in shaping development approaches. Although expertise from the oil and gas industry can accelerate technological progress in marine geothermal energy, economic challenges remain. Therefore, improving cost competitiveness is crucial for offshore geothermal energy. Full article

Objectives: The aim of this study was to evaluate the impact of a novel antioxidant formulation (RE:PAIR, RP-25) containing CoQ10, alpha-lipoic acid, and Chaga extract on mitochondrial dysfunction and oxidative stress. To explore the activity of the formulation on neuronal cells, we explored cell metabolism and its activity as an antioxidant, using a combination of NMR-based metabolomics and UHPLC-HRMS analytical techniques. Methods: SH-SY5Y neuroblastoma cells were treated with RP-25, and cell viability was assessed via CCK-8 assay. Metabolomic profiles of the treated and untreated cells were analyzed by 1D-NMR, providing insights into both intracellular metabolites (endometabolome) and excreted metabolites (exometabolome). Additionally, a UHPLC-HRMS method was developed for quality control and analysis of the RP-25 formulation. Multivariate statistical approaches, including PLS-DA and volcano plot analyses, were used to identify key metabolic changes. Changes in mitochondrial membrane potential were assessed by means of TMRE assay, while radical oxygen species (ROS) were measured by means of the DCHF assay. Results: RP-25 treatment did not affect cell viability but significantly increased metabolic pathways, including amino acid biosynthesis, oxidative phosphorylation, and glycolysis. Higher levels of ATP, glutamate, tyrosine, and proline were observed in treated cells than in control cells, indicating enhanced cellular energy production, as also proved by the increased stability of the mitochondrial membrane after RP-25 treatment, an index of preserved mitochondrial functions. In support, the formulation RP-25 showed antioxidant activity when cells underwent peroxide oxygen stimulation. This effect was mainly due to the combination of Chaga, CoQ10, and ALA, main components of the RP25 formulation. Moreover, the analysis of enriched pathways highlighted that RP formulation influenced mitochondrial energy and oxidative stress response. Conclusions: RP-25 demonstrated biological activity in that it mitigated mitochondrial dysfunction and oxidative stress in neuronal cells, with potential implications in neuronal diseases associated with dysfunctional mitochondria. Full article

This study aimed to investigate the effects of shear stress on osteogenic differentiation of human dental pulp stem cells (hDPSCs). The hDPSCs were subjected to shear stress for 24 h before osteogenic induction for 21 days. The mRNA expression of osteogenic markers such as RUNX2, OSX, ALP, COL1A1, OCN, and OPN was evaluated by real-time RT-PCR. Alkaline Phosphatase (ALP) activity and Alizarin Red S (ARS) staining were investigated to confirm osteogenic differentiation and mineralization of hDPSCs, respectively. The protein expression of osterix was shown by immunofluorescence staining and Western blotting. RNA sequencing was performed to investigate how shear stress affects the osteogenic differentiation of hDPSCs, which was validated through p38 inhibitor (SB203580) treatment. Real-time RT-PCR revealed that shear stress enhanced osteogenic marker-gene expression. The increased osterix protein expression was detected on Day 14 in the shear-stress loading group compared to the static group. Shear stress enhanced ALP activity and mineralization, observed on Days 14 and 21. A volcano plot exhibited up- and downregulated genes, while the p38 inhibitor markedly inhibited osteogenic differentiation of hDPSCs triggered by shear stress. In conclusion, shear stress promotes the osteogenic differentiation of hDPSCs through the p38 mitogen-activated protein kinase signaling pathway. Full article

The Orbiting Carbon Observatory-3 (OCO-3) mission provides a new perspective for studying atmospheric carbon dioxide (CO₂). Here we assess the potentiality of OCO-3 satellite acquisitions to analyse and monitor the CO₂ emissions from Mt. Etna volcano. While OCO-3 data are well-suited for gas analysis on a regional spatial scale, they have not yet been widely utilised for studying volcanic carbon dioxide emissions. The Snapshot Area Map (SAM) acquisition mode enables the capture of targeted snapshots over volcanic regions, allowing for the measurement of CO₂ concentrations in the vicinity of volcanic structures. In this work, we analyse 62 OCO-3 images acquired between 2020 and 2023, focusing on measurements within a 20 km radius of Mt. Etna’s summit, where the main craters are located. Atmospheric CO₂ concentrations are examined as a function of distance from the summit, and assuming a linear decreasing trend, the angular coefficient is computed. Lower angular coefficient values may indicate a stronger volcanic CO₂ contribution. Considering both the number of sampled pixels in each OCO-3 snapshot and the associated uncertainties in the angular coefficient calculation, we identify five days with potentially significant CO₂ emissions from Mt. Etna, likely associated with specific volcanic activity phases. The eruptive activity on these five days is further investigated, revealing a possible correlation between elevated gas emissions and intense volcanic phenomena, such as lava fountains. This assessment is supported by thermal activity analyses using SEVIRI, MODIS, and VIIRS satellite data. Full article

Globally, most skarn deposits show a direct relationship with magmatic activity, indicating a genetic link between the geochemical composition of causative plutons and the metal content of associated skarns. Therefore, this study investigated the Early–Middle Eocene plutonic rocks and their relationship with Fe-Zn skarn deposits in the Esendemirtepe-Koçak and Horoz areas of south-central Türkiye. Despite the regional significance, previous studies have not adequately addressed the petrogenetic evolution of these intrusions and the geochemical characteristics of the related skarns. In particular, the fluid-aided mobility of elements at the contact between the causative plutons and the volcano-sedimentary country rocks remains poorly understood. Therefore, in this study, field studies, petrographic and mineralogical analysis, and whole-rock geochemical analysis were conducted to investigate the genetic link between the plutonic rocks and the skarn deposits. Field studies reveal that the skarn zones are within volcano-sedimentary sequences and marble-schist units intruded by four distinct plutonic bodies: (1) Esendemirtepe diorite, (2) Koçak diorite, (3) Horoz granodiorite, and (4) Çifteköy monzogabbro. These rocks exhibit calc-alkaline, I-type, and metaluminous signatures, except for the Çifteköy monzogabbro, which shows I-type, tholeiitic, and alkaline characteristics. All the plutonic rocks associated with the skarn formation display steep LREE-enriched REE patterns with minor positive Eu anomalies (Eu/Eu* = 0.98–1.35), suggesting a subduction-related volcanic arc setting similar to other granitoids in the Ulukışla Basin. The Horoz skarn exhibits both endoskarn and exoskarn features, while the Esendemirtepe-Koçak deposit is characterized by typical exoskarn features. Dominant ore minerals in both skarn deposits include magnetite, hematite, sphalerite, chalcopyrite, and pyrite, with minor arsenopyrite, galena, and cobaltite. The mineral composition of the skarn also shows the dominance of Na-rich and Mg-rich minerals in both locations. The geochemical compositions of the I-type, metaluminous Esendemirtepe-Koçak, and Horoz plutonic rocks are compatible with Fe-Zn skarn type deposits based on the moderate MgO (0.36–4.44 wt.%) and K₂O (1.38–7.99 wt.%), and Rb/Zr and Sr/Zr ratios. They also show typical volcanic arc features, and the variation in various trace element concentrations shows similarity with Fe-Zn skarn type granitoids. These findings support a strong genetic relationship between the mineralization and the geochemical and mineralogical characteristics of the associated plutonic rocks. Full article

This study investigates the impact of volcanic ash from Cotopaxi Volcano on the nutritional quality and biological traits of potato tubers (Solanum tuberosum L.) cultivated in the Cotopaxi region. Methods: Samples collected from exposed and unexposed areas were used to characterize the volcanic ash influence on the metabolic aspects of the potato crop. The colorimetric method; DPPH, ABTS, and FRAP assays; and ICP-OES were used to better understand potatoes’ reaction to the stress. Results: Antioxidant activity was significantly higher (4.80 ± 2.38 µmol Trolox g⁻¹ DW-DPPH assay; 11.05 ± 2.57 µmol Trolox g⁻¹ DW-ABTS assay; and 11.96 ± 4.57 µmol Fe²⁺ g⁻¹ DW-FRAP assay) in ash-exposed samples, suggesting enhanced stress responses. The bioactive compounds studied followed a comparable trend, with high content in the exposed tubers. Also, significant changes in elemental composition were registered: Potassium levels decreased in unexposed samples, while magnesium and iron levels increased. Metallic elements (zinc; lithium; boron; manganese; barium; lead; nickel; chromium; indium) were in concentrations <0.01 mg/kg. Conclusion: These findings demonstrate that volcanic ash alters the metabolic and antioxidant profiles of potato tubers, enhancing nutraceutical properties while posing food safety risks due to heavy metals. This dual impact highlights the challenges and opportunities for agriculture in volcanic regions like Cotopaxi. Full article

Papillary thyroid cancer (PTC) is the most common thyroid malignancy, with significant racial/ethnic disparities in incidence and survival. Asians have the highest incidence, and recurrence, while African Americans experience the lowest survival rates, suggesting contributions from genetic, environmental, and healthcare-related factors. While socioeconomic disparities play a role, emerging evidence highlights genetic and molecular mechanisms underlying these differences. This study examines differentially expressed genes (DEGs) to identify potential molecular drivers of PTC disparities. Bulk RNA-sequencing (RNA-seq) data from 20 PTC tumors (5 White, 5 African American, 5 Hispanic, and 5 Asian) were analyzed using the UseGalaxy platform. Preprocessing included quality control, adapter trimming, and genome alignment. Differential expression analysis identified genes with p < 0.01 and fold change ≥ 2.5. Volcano plots visualized significant DEGs. Gene Set Enrichment Analysis (GSEA) via eVITTA identified enriched pathways. TCGA data analysis validated racial/ethnic differences in gene expression. Ethnic groups exhibited distinct gene expression profiles. GSEA revealed differences in cell proliferation, immune regulation, and thyroid hormone metabolism. African Americans showed immune suppression and reduced tumor suppressor activity, while Asians exhibited enriched cell cycle and DNA repair pathways. Significant differences were confirmed in some of the genes in TCGA data analysis. This study identifies genetic factors contributing to racial disparities in PTC, emphasizing the need for further validation in larger cohorts and functional studies. Understanding these molecular differences may inform personalized treatment strategies and improve PTC outcomes across diverse populations. Full article

In recent years, numerous satellite-based systems have been developed to monitor and study volcanic activity from space. This progress reflects the growing demand for accurate and timely monitoring to reduce volcanic risk. Observing volcanoes from a satellite perspective provides key advantages, enabling continuous data acquisition and near-real-time assessment of volcanic activity. Multispectral sensors operating across various regions of the electromagnetic spectrum can detect thermal anomalies associated with lava flows, pyroclastic flows, ash plumes, and volcanic gases. Traditional hotspot detection techniques based on fixed thresholds often miss subtle anomalies on a global scale. In contrast, advanced machine learning algorithms offer a data-driven alternative. We designed and implemented the V-STAR application (Volcanic Satellite Thermal Anomalies Recognition) on Google Earth Engine (GEE) to leverage cloud computing for processing large geospatial datasets in real time. It employs supervised machine learning, specifically Random Forests, to adapt to evolving volcanic conditions. This enhances the accuracy and responsiveness of volcanic monitoring, offering valuable insights into potential eruptive behavior. Here, we present V-STAR as a robust and accessible tool that integrates satellite data and advanced analytics. Through its intuitive interface, V-STAR provides a comprehensive visualization of key volcanic features. The resulting analyses reveal hidden patterns in thermal data, contributing to improved disaster risk reduction strategies associated with volcanic hazards. Full article