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8 pages, 5870 KiB  
Proceeding Paper
Classification of Urban Environments Using State-of-the-Art Machine Learning: A Path to Sustainability
by Tesfaye Tessema, Neda Azarmehr, Parisa Saadati, Dale Mortimer and Fabio Tosti
Eng. Proc. 2025, 94(1), 14; https://doi.org/10.3390/engproc2025094014 - 4 Aug 2025
Abstract
Urban green infrastructure plays a vital role in the sustainable development of cities. As urban areas expand, green spaces are increasingly affected. The pressure from new developments leads to a reduction in vegetation and raises new public health risks. Addressing this challenge requires [...] Read more.
Urban green infrastructure plays a vital role in the sustainable development of cities. As urban areas expand, green spaces are increasingly affected. The pressure from new developments leads to a reduction in vegetation and raises new public health risks. Addressing this challenge requires effective planning, maintenance, and continuous monitoring. To enhance traditional approaches, remote sensing is becoming a vital tool for city-wide observations. Publicly available large-scale data, combined with machine learning models, can improve our understanding. We explore the potential of Sentinel-2 to classify and extract meaningful features from urban landscapes. Using advanced machine learning techniques, we aim to develop a robust and scalable framework for classifying urban environments. The proposed models will assist in monitoring changes in green spaces across diverse urban settings, enabling timely and informed decisions to foster sustainable urban growth. Full article
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21 pages, 5068 KiB  
Article
Estimating Household Green Space in Composite Residential Community Solely Using Drone Oblique Photography
by Meiqi Kang, Kaiyi Song, Xiaohan Liao and Jiayuan Lin
Remote Sens. 2025, 17(15), 2691; https://doi.org/10.3390/rs17152691 - 3 Aug 2025
Viewed by 117
Abstract
Residential green space is an important component of urban green space and one of the major indicators for evaluating the quality of a residential community. Traditional indicators such as the green space ratio only consider the relationship between green space area and total [...] Read more.
Residential green space is an important component of urban green space and one of the major indicators for evaluating the quality of a residential community. Traditional indicators such as the green space ratio only consider the relationship between green space area and total area of the residential community while ignoring the difference in the amount of green space enjoyed by household residents in high-rise and low-rise buildings. Therefore, it is meaningful to estimate household green space and its spatial distribution in residential communities. However, there are frequent difficulties in obtaining specific green space area and household number through ground surveys or consulting with property management units. In this study, taking a composite residential community in Chongqing, China, as the study site, we first employed a five-lens drone to capture its oblique RGB images and generated the DOM (Digital Orthophoto Map). Subsequently, the green space area and distribution in the entire residential community were extracted from the DOM using VDVI (Visible Difference Vegetation Index). The YOLACT (You Only Look At Coefficients) instance segmentation model was used to recognize balconies from the facade images of high-rise buildings to determine their household numbers. Finally, the average green space per household in the entire residential community was calculated to be 67.82 m2, and those in the high-rise and low-rise building zones were 51.28 m2 and 300 m2, respectively. Compared with the green space ratios of 65.5% and 50%, household green space more truly reflected the actual green space occupation in high- and low-rise building zones. Full article
(This article belongs to the Special Issue Application of Remote Sensing in Landscape Ecology)
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21 pages, 4314 KiB  
Article
Panoptic Plant Recognition in 3D Point Clouds: A Dual-Representation Learning Approach with the PP3D Dataset
by Lin Zhao, Sheng Wu, Jiahao Fu, Shilin Fang, Shan Liu and Tengping Jiang
Remote Sens. 2025, 17(15), 2673; https://doi.org/10.3390/rs17152673 - 2 Aug 2025
Viewed by 214
Abstract
The advancement of Artificial Intelligence (AI) has significantly accelerated progress across various research domains, with growing interest in plant science due to its substantial economic potential. However, the integration of AI with digital vegetation analysis remains underexplored, largely due to the absence of [...] Read more.
The advancement of Artificial Intelligence (AI) has significantly accelerated progress across various research domains, with growing interest in plant science due to its substantial economic potential. However, the integration of AI with digital vegetation analysis remains underexplored, largely due to the absence of large-scale, real-world plant datasets, which are crucial for advancing this field. To address this gap, we introduce the PP3D dataset—a meticulously labeled collection of about 500 potted plants represented as 3D point clouds, featuring fine-grained annotations for approximately 20 species. The PP3D dataset provides 3D phenotypic data for about 20 plant species spanning model organisms (e.g., Arabidopsis thaliana), potted plants (e.g., Foliage plants, Flowering plants), and horticultural plants (e.g., Solanum lycopersicum), covering most of the common important plant species. Leveraging this dataset, we propose the panoptic plant recognition task, which combines semantic segmentation (stems and leaves) with leaf instance segmentation. To tackle this challenge, we present SCNet, a novel dual-representation learning network designed specifically for plant point cloud segmentation. SCNet integrates two key branches: a cylindrical feature extraction branch for robust spatial encoding and a sequential slice feature extraction branch for detailed structural analysis. By efficiently propagating features between these representations, SCNet achieves superior flexibility and computational efficiency, establishing a new baseline for panoptic plant recognition and paving the way for future AI-driven research in plant science. Full article
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16 pages, 3038 KiB  
Article
Comparative Effects of Maturity and Processing on Chemical Composition and Bioactivities in Toona sinensis Leaves
by Guohuo Wu, Zhaoyun Chen, Yan Tang, Shuolei Xu, Wenli Fan, Li Wu, Yuntao Ji and Changqing Qu
Foods 2025, 14(15), 2717; https://doi.org/10.3390/foods14152717 - 2 Aug 2025
Viewed by 253
Abstract
Toona sinensis (“Heiyouchun”) is a traditional Chinese woody vegetable, the leaves of which can also be processed into tea, known for its distinctive flavor and diverse bioactivities. However, the effects of leaf maturity and processing methods on its phytochemical composition and functional properties [...] Read more.
Toona sinensis (“Heiyouchun”) is a traditional Chinese woody vegetable, the leaves of which can also be processed into tea, known for its distinctive flavor and diverse bioactivities. However, the effects of leaf maturity and processing methods on its phytochemical composition and functional properties remain unclear. In this study, metabolomic analysis revealed 35 significantly different metabolites between tender and mature leaves, with higher concentrations of flavonoids, flavonoid glycosides, limonoids, and amino acids in tender leaves. Additionally, comparative analysis revealed that black tea fermentation preserves bioactive compounds more effectively than hot-air drying, particularly in tender leaves. In vitro activity assays showed that toon leaf tea extracts exhibited significant antioxidant and hypoglycemic effects, with black tea fermented tender leaves displaying the most potent bioactivity. Correlation analysis further confirmed a strong positive relationship between flavonoid/polyphenol content and bioactivity. These findings provide a theoretical foundation for optimizing processing techniques to enhance the functional properties of toon leaf tea. Full article
(This article belongs to the Section Plant Foods)
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19 pages, 1134 KiB  
Article
Application of Animal- and Plant-Derived Coagulant in Artisanal Italian Caciotta Cheesemaking: Comparison of Sensory, Biochemical, and Rheological Parameters
by Giovanna Lomolino, Stefania Zannoni, Mara Vegro and Alberto De Iseppi
Dairy 2025, 6(4), 43; https://doi.org/10.3390/dairy6040043 - 1 Aug 2025
Viewed by 88
Abstract
Consumer interest in vegetarian, ethical, and clean-label foods is reviving the use of plant-derived milk coagulants. Cardosins from Cynara cardunculus (“thistle”) are aspartic proteases with strong clotting activity, yet their technological impact in cheese remains under-explored. This study compared a commercial thistle extract [...] Read more.
Consumer interest in vegetarian, ethical, and clean-label foods is reviving the use of plant-derived milk coagulants. Cardosins from Cynara cardunculus (“thistle”) are aspartic proteases with strong clotting activity, yet their technological impact in cheese remains under-explored. This study compared a commercial thistle extract (PC) with traditional bovine rennet rich in chymosin (AC) during manufacture and 60-day ripening of Caciotta cheese. Classical compositional assays (ripening index, texture profile, color, solubility) were integrated with scanning electron microscopy, three-dimensional surface reconstruction, and descriptive sensory analysis. AC cheeses displayed slower but sustained proteolysis, yielding a higher and more linear ripening index, softer body, greater solubility, and brighter, more yellow appearance. Imaging revealed a continuous protein matrix with uniformly distributed, larger pores, consistent with a dairy-like sensory profile dominated by milky and umami notes. Conversely, PC cheeses underwent rapid early proteolysis that plateaued, producing firmer, chewier curds with lower solubility and darker color. Micrographs showed a fragmented matrix with smaller, heterogeneous pores; sensory evaluation highlighted vegetal, bitter, and astringent attributes. The data demonstrate that thistle coagulant can successfully replace animal rennet but generates cheeses with distinct structural and sensory fingerprints. The optimization of process parameters is therefore required when targeting specific product styles. Full article
(This article belongs to the Section Milk Processing)
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22 pages, 8105 KiB  
Article
Extraction of Sparse Vegetation Cover in Deserts Based on UAV Remote Sensing
by Jie Han, Jinlei Zhu, Xiaoming Cao, Lei Xi, Zhao Qi, Yongxin Li, Xingyu Wang and Jiaxiu Zou
Remote Sens. 2025, 17(15), 2665; https://doi.org/10.3390/rs17152665 - 1 Aug 2025
Viewed by 200
Abstract
The unique characteristics of desert vegetation, such as different leaf morphology, discrete canopy structures, sparse and uneven distribution, etc., pose significant challenges for remote sensing-based estimation of fractional vegetation cover (FVC). The Unmanned Aerial Vehicle (UAV) system can accurately distinguish vegetation patches, extract [...] Read more.
The unique characteristics of desert vegetation, such as different leaf morphology, discrete canopy structures, sparse and uneven distribution, etc., pose significant challenges for remote sensing-based estimation of fractional vegetation cover (FVC). The Unmanned Aerial Vehicle (UAV) system can accurately distinguish vegetation patches, extract weak vegetation signals, and navigate through complex terrain, making it suitable for applications in small-scale FVC extraction. In this study, we selected the floodplain fan with Caragana korshinskii Kom as the constructive species in Hatengtaohai National Nature Reserve, Bayannur, Inner Mongolia, China, as our study area. We investigated the remote sensing extraction method of desert sparse vegetation cover by placing samples across three gradients: the top, middle, and edge of the fan. We then acquired UAV multispectral images; evaluated the applicability of various vegetation indices (VIs) using methods such as supervised classification, linear regression models, and machine learning; and explored the feasibility and stability of multiple machine learning models in this region. Our results indicate the following: (1) We discovered that the multispectral vegetation index is superior to the visible vegetation index and more suitable for FVC extraction in vegetation-sparse desert regions. (2) By comparing five machine learning regression models, it was found that the XGBoost and KNN models exhibited relatively lower estimation performance in the study area. The spatial distribution of plots appeared to influence the stability of the SVM model when estimating fractional vegetation cover (FVC). In contrast, the RF and LASSO models demonstrated robust stability across both training and testing datasets. Notably, the RF model achieved the best inversion performance (R2 = 0.876, RMSE = 0.020, MAE = 0.016), indicating that RF is one of the most suitable models for retrieving FVC in naturally sparse desert vegetation. This study provides a valuable contribution to the limited existing research on remote sensing-based estimation of FVC and characterization of spatial heterogeneity in small-scale desert sparse vegetation ecosystems dominated by a single species. Full article
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40 pages, 1885 KiB  
Review
Potential Application of Plant By-Products in Biomedicine: From Current Knowledge to Future Opportunities
by Silvia Estarriaga-Navarro, Teresa Valls, Daniel Plano, Carmen Sanmartín and Nieves Goicoechea
Antioxidants 2025, 14(8), 942; https://doi.org/10.3390/antiox14080942 (registering DOI) - 31 Jul 2025
Viewed by 291
Abstract
Plant by-products have gained significant attention due to their rich content in bioactive compounds, which exhibit promising antioxidant, antimicrobial, and antitumor properties. In European countries, vegetable waste generation ranged from 35 to 78 kg per capita in 2022, highlighting both the scale of [...] Read more.
Plant by-products have gained significant attention due to their rich content in bioactive compounds, which exhibit promising antioxidant, antimicrobial, and antitumor properties. In European countries, vegetable waste generation ranged from 35 to 78 kg per capita in 2022, highlighting both the scale of the challenge and the potential for valorization. This review provides an overview of key studies investigating the potential of plant residues in biomedicine, highlighting their possible contents of antioxidant compounds, their antimicrobial and antitumor properties, as well as their applications in dermocosmetics and nutraceuticals. However, despite their potential, several challenges must be addressed, such as the standardization of extraction protocols, as bioactive compound profiles can vary with plant source, processing conditions, and storage methods. Effective segregation and storage protocols for household organic waste also require optimization to ensure the quality and usability of plant by-products in biomedicine. Emerging 4.0 technologies could help to identify suitable plant by-products for biomedicine, streamlining their selection process for high-value applications. Additionally, the transition from in vitro studies to clinical trials is hindered by gaps in the understanding of Absorption, Distribution, Metabolism, and Excretion (ADME) properties, as well as interaction and toxicity profiles. Nonetheless, environmental education and societal participation are crucial to enabling circular bioeconomy strategies and sustainable biomedical innovation. Full article
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20 pages, 4117 KiB  
Review
Analytical Strategies for Tocopherols in Vegetable Oils: Advances in Extraction and Detection
by Yingfei Liu, Mengyuan Lv, Yuyang Wang, Jinchao Wei and Di Chen
Pharmaceuticals 2025, 18(8), 1137; https://doi.org/10.3390/ph18081137 - 30 Jul 2025
Viewed by 198
Abstract
Tocopherols, major lipid-soluble components of vitamin E, are essential natural products with significant nutritional and pharmacological value. Their structural diversity and uneven distribution across vegetable oils require accurate analytical strategies for compositional profiling, quality control, and authenticity verification, amid concerns over food fraud [...] Read more.
Tocopherols, major lipid-soluble components of vitamin E, are essential natural products with significant nutritional and pharmacological value. Their structural diversity and uneven distribution across vegetable oils require accurate analytical strategies for compositional profiling, quality control, and authenticity verification, amid concerns over food fraud and regulatory demands. Analytical challenges, such as matrix effects in complex oils and the cost trade-offs of green extraction methods, complicate these processes. This review examines recent advances in tocopherol analysis, focusing on extraction and detection techniques. Green methods like supercritical fluid extraction and deep eutectic solvents offer selectivity and sustainability, though they are costlier than traditional approaches. On the analytical side, hyphenated techniques such as supercritical fluid chromatography-mass spectrometry (SFC-MS) achieve detection limits as low as 0.05 ng/mL, improving sensitivity in complex matrices. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) provides robust analysis, while spectroscopic and electrochemical sensors offer rapid, cost-effective alternatives for high-throughput screening. The integration of chemometric tools and miniaturized systems supports scalable workflows. Looking ahead, the incorporation of Artificial Intelligence (AI) in oil authentication has the potential to enhance the accuracy and efficiency of future analyses. These innovations could improve our understanding of tocopherol compositions in vegetable oils, supporting more reliable assessments of nutritional value and product authenticity. Full article
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26 pages, 8762 KiB  
Article
Clustered Rainfall-Induced Landslides in Jiangwan Town, Guangdong, China During April 2024: Characteristics and Controlling Factors
by Ruizeng Wei, Yunfeng Shan, Lei Wang, Dawei Peng, Ge Qu, Jiasong Qin, Guoqing He, Luzhen Fan and Weile Li
Remote Sens. 2025, 17(15), 2635; https://doi.org/10.3390/rs17152635 - 29 Jul 2025
Viewed by 227
Abstract
On 20 April 2024, an extreme rainfall event occurred in Jiangwan Town Shaoguan City, Guangdong Province, China, where a historic 24 h precipitation of 206 mm was recorded. This triggered extensive landslides that destroyed residential buildings, severed roads, and drew significant societal attention. [...] Read more.
On 20 April 2024, an extreme rainfall event occurred in Jiangwan Town Shaoguan City, Guangdong Province, China, where a historic 24 h precipitation of 206 mm was recorded. This triggered extensive landslides that destroyed residential buildings, severed roads, and drew significant societal attention. Rapid acquisition of landslide inventories, distribution patterns, and key controlling factors is critical for post-disaster emergency response and reconstruction. Based on high-resolution Planet satellite imagery, landslide areas in Jiangwan Town were automatically extracted using the Normalized Difference Vegetation Index (NDVI) differential method, and a detailed landslide inventory was compiled. Combined with terrain, rainfall, and geological environmental factors, the spatial distribution and causes of landslides were analyzed. Results indicate that the extreme rainfall induced 1426 landslides with a total area of 4.56 km2, predominantly small-to-medium scale. Landslides exhibited pronounced clustering and linear distribution along river valleys in a NE–SW orientation. Spatial analysis revealed concentrations on slopes between 200–300 m elevation with gradients of 20–30°. Four machine learning models—Logistic Regression, Support Vector Machine (SVM), Random Forest (RF), and Extreme Gradient Boosting (XGBoost)—were employed to assess landslide susceptibility mapping (LSM) accuracy. RF and XGBoost demonstrated superior performance, identifying high-susceptibility zones primarily on valley-side slopes in Jiangwan Town. Shapley Additive Explanations (SHAP) value analysis quantified key drivers, highlighting elevation, rainfall intensity, profile curvature, and topographic wetness index as dominant controlling factors. This study provides an effective methodology and data support for rapid rainfall-induced landslide identification and deep learning-based susceptibility assessment. Full article
(This article belongs to the Special Issue Study on Hydrological Hazards Based on Multi-Source Remote Sensing)
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19 pages, 4467 KiB  
Article
Delineation of Dynamic Coastal Boundaries in South Africa from Hyper-Temporal Sentinel-2 Imagery
by Mariel Bessinger, Melanie Lück-Vogel, Andrew Luke Skowno and Ferozah Conrad
Remote Sens. 2025, 17(15), 2633; https://doi.org/10.3390/rs17152633 - 29 Jul 2025
Viewed by 160
Abstract
The mapping and monitoring of coastal regions are critical to ensure their sustainable use and viability in the long term. Delineation of coastlines is becoming increasingly important in the light of climate change and rising sea levels. However, many coastlines are highly dynamic; [...] Read more.
The mapping and monitoring of coastal regions are critical to ensure their sustainable use and viability in the long term. Delineation of coastlines is becoming increasingly important in the light of climate change and rising sea levels. However, many coastlines are highly dynamic; therefore, mono-temporal assessments of coastal ecosystems and coastlines are mere snapshots of limited practical value for space-based planning. Understanding of the spatio-temporal dynamics of coastal ecosystem boundaries is important to inform ecosystem management but also for a meaningful delineation of the high-water mark, which is used as a benchmark for coastal spatial planning in South Africa. This research aimed to use hyper-temporal Sentinel-2 imagery to extract ecological zones on the coast of KwaZulu-Natal, South Africa. A total of 613 images, collected between 2019 and 2023, were classified into four distinct coastal ecological zones—vegetation, bare, surf, and water—using a Random Forest model. Across all classifications, the percentage of each of the four classes’ occurrence per pixel over time was determined. This enabled the identification of ecosystem locations, spatially static ecosystem boundaries, and the occurrence of ecosystem boundaries with a more dynamic location over time, such as the non-permanent vegetation zone of the foredune area as well as the intertidal zone. The overall accuracy of the model was 98.13%, while the Kappa coefficient was 0.975, with user’s and producer’s accuracies ranging between 93.02% and 100%. These results indicate that cloud-based analysis of Sentinel-2 time series holds potential not just for delineating coastal ecosystem boundaries, but also for enhancing the understanding of spatio-temporal dynamics between them, to inform meaningful environmental management, spatial planning, and climate adaptation strategies. Full article
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28 pages, 7240 KiB  
Article
MF-FusionNet: A Lightweight Multimodal Network for Monitoring Drought Stress in Winter Wheat Based on Remote Sensing Imagery
by Qiang Guo, Bo Han, Pengyu Chu, Yiping Wan and Jingjing Zhang
Agriculture 2025, 15(15), 1639; https://doi.org/10.3390/agriculture15151639 - 29 Jul 2025
Viewed by 257
Abstract
To improve the identification of drought-affected areas in winter wheat, this paper proposes a lightweight network called MF-FusionNet based on multimodal fusion of RGB images and vegetation indices (NDVI and EVI). A multimodal dataset covering various drought levels in winter wheat was constructed. [...] Read more.
To improve the identification of drought-affected areas in winter wheat, this paper proposes a lightweight network called MF-FusionNet based on multimodal fusion of RGB images and vegetation indices (NDVI and EVI). A multimodal dataset covering various drought levels in winter wheat was constructed. To enable deep fusion of modalities, a Lightweight Multimodal Fusion Block (LMFB) was designed, and a Dual-Coordinate Attention Feature Extraction module (DCAFE) was introduced to enhance semantic feature representation and improve drought region identification. To address differences in scale and semantics across network layers, a Cross-Stage Feature Fusion Strategy (CFFS) was proposed to integrate multi-level features and enhance overall performance. The effectiveness of each module was validated through ablation experiments. Compared to traditional single-modal methods, MF-FusionNet achieved higher accuracy, recall, and F1-score—improved by 1.35%, 1.43%, and 1.29%, respectively—reaching 96.71%, 96.71%, and 96.64%. A basis for real-time monitoring and precise irrigation management under winter wheat drought stress was provided by this study. Full article
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24 pages, 4396 KiB  
Article
Study of the Characteristics of a Co-Seismic Displacement Field Based on High-Resolution Stereo Imagery: A Case Study of the 2024 MS7.1 Wushi Earthquake, Xinjiang
by Chenyu Ma, Zhanyu Wei, Li Qian, Tao Li, Chenglong Li, Xi Xi, Yating Deng and Shuang Geng
Remote Sens. 2025, 17(15), 2625; https://doi.org/10.3390/rs17152625 - 29 Jul 2025
Viewed by 263
Abstract
The precise characterization of surface rupture zones and associated co-seismic displacement fields from large earthquakes provides critical insights into seismic rupture mechanisms, earthquake dynamics, and hazard assessments. Stereo-photogrammetric digital elevation models (DEMs), produced from high-resolution satellite stereo imagery, offer reliable global datasets that [...] Read more.
The precise characterization of surface rupture zones and associated co-seismic displacement fields from large earthquakes provides critical insights into seismic rupture mechanisms, earthquake dynamics, and hazard assessments. Stereo-photogrammetric digital elevation models (DEMs), produced from high-resolution satellite stereo imagery, offer reliable global datasets that are suitable for the detailed extraction and quantification of vertical co-seismic displacements. In this study, we utilized pre- and post-event WorldView-2 stereo images of the 2024 Ms7.1 Wushi earthquake in Xinjiang to generate DEMs with a spatial resolution of 0.5 m and corresponding terrain point clouds with an average density of approximately 4 points/m2. Subsequently, we applied the Iterative Closest Point (ICP) algorithm to perform differencing analysis on these datasets. Special care was taken to reduce influences from terrain changes such as vegetation growth and anthropogenic structures. Ultimately, by maintaining sufficient spatial detail, we obtained a three-dimensional co-seismic displacement field with a resolution of 15 m within grid cells measuring 30 m near the fault trace. The results indicate a clear vertical displacement distribution pattern along the causative sinistral–thrust fault, exhibiting alternating uplift and subsidence zones that follow a characteristic “high-in-center and low-at-ends” profile, along with localized peak displacement clusters. Vertical displacements range from approximately 0.2 to 1.4 m, with a maximum displacement of ~1.46 m located in the piedmont region north of the Qialemati River, near the transition between alluvial fan deposits and bedrock. Horizontal displacement components in the east-west and north-south directions are negligible, consistent with focal mechanism solutions and surface rupture observations from field investigations. The successful extraction of this high-resolution vertical displacement field validates the efficacy of satellite-based high-resolution stereo-imaging methods for overcoming the limitations of GNSS and InSAR techniques in characterizing near-field surface displacements associated with earthquake ruptures. Moreover, this dataset provides robust constraints for investigating fault-slip mechanisms within near-surface geological contexts. Full article
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17 pages, 2025 KiB  
Article
Retainment of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Properties from Oil-Fermented Cupriavidus necator Using Additional Ethanol-Based Defatting Process
by Tae-Rim Choi, Gaeun Lim, Yebin Han, Jong-Min Jeon, Shashi Kant Bhatia, Hyun June Park, Jeong Chan Joo, Hee Taek Kim, Jeong-Jun Yoon and Yung-Hun Yang
Polymers 2025, 17(15), 2058; https://doi.org/10.3390/polym17152058 - 28 Jul 2025
Viewed by 295
Abstract
Engineering of Cupriavidus necator could enable the production of various polyhydroxyalkanoates (PHAs); particularly, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)), a biopolymer with enhanced mechanical and thermal properties compared to poly(3-hydroxybutyrate) (PHB), can be efficiently produced from vegetable oils. However, challenges remain in the [...] Read more.
Engineering of Cupriavidus necator could enable the production of various polyhydroxyalkanoates (PHAs); particularly, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)), a biopolymer with enhanced mechanical and thermal properties compared to poly(3-hydroxybutyrate) (PHB), can be efficiently produced from vegetable oils. However, challenges remain in the recovery process, particularly in removing residual oil and minimizing degradation of the polymer structure during extraction steps. This study investigated the effects of ethanol-based defatting on the recovery and polymeric properties of P(3HB-co-3HH). The proposed method involves the addition of ethanol to the cell broth to effectively remove residual oil. Ethanol improved the separation of microbial cells from the broth, thereby streamlining the downstream recovery process. Using ethanol in the washing step increased the recovery yield and purity to 95.7% and 83.4%, respectively (compared to 87.4% and 76.2% for distilled water washing), representing improvements of 8.3% and 7.2%. Ethanol washing also resulted in a 19% higher molecular weight compared to water washing, indicating reduced polymer degradation. In terms of physical properties, the elongation at break showed a significant difference: 241.9 ± 27.0% with ethanol washing compared to water (177.7 ± 10.3%), indicating ethanol washing retains flexibility. Overall, an ethanol washing step for defatting could simplify the recovery steps, increase yield and purity, and retain mechanical properties, especially for P(3HB-co-3HH) from oils. Full article
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14 pages, 635 KiB  
Review
Methods of Control of Parasitic Weeds of the Genus Cuscuta—Current Status and Future Perspectives
by Lyuben Zagorchev, Tzvetelina Zagorcheva, Denitsa Teofanova and Mariela Odjakova
Plants 2025, 14(15), 2321; https://doi.org/10.3390/plants14152321 - 27 Jul 2025
Viewed by 469
Abstract
Dodders (Cuscuta spp.; Convolvulaceae) are parasitic weeds that pose major challenges to agriculture due to their ability to infect a wide range of host plants, extract nutrients, and transmit pathogens. Their control is especially challenging because of the seed longevity, resistance to [...] Read more.
Dodders (Cuscuta spp.; Convolvulaceae) are parasitic weeds that pose major challenges to agriculture due to their ability to infect a wide range of host plants, extract nutrients, and transmit pathogens. Their control is especially challenging because of the seed longevity, resistance to herbicides, and the capacity for vegetative regeneration. Mechanical methods such as hand-pulling or mowing are labour-intensive and often ineffective for large infestations. Chemical control is limited, as systemic herbicides often affect the host species equally, or even worse than the parasite. Current research is exploring biological control methods, including allelopathic compounds, host-specific fungal pathogens, and epiparasitic insects, though these methods remain largely experimental. An integrated approach that combines prevention, targeted mechanical removal, and biological methods offers the most promising path for long-term management. Continued research is essential to develop effective, sustainable control strategies while exploring possible beneficial uses of these complex parasitic plants. The present review aims to thoroughly summarise the existing literature, emphasising the most recent advances and discussing future perspectives. Full article
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24 pages, 5039 KiB  
Article
Advanced Estimation of Winter Wheat Leaf’s Relative Chlorophyll Content Across Growth Stages Using Satellite-Derived Texture Indices in a Region with Various Sowing Dates
by Jingyun Chen, Quan Yin, Jianjun Wang, Weilong Li, Zhi Ding, Pei Sun Loh, Guisheng Zhou and Zhongyang Huo
Plants 2025, 14(15), 2297; https://doi.org/10.3390/plants14152297 - 25 Jul 2025
Viewed by 275
Abstract
Accurately estimating leaves’ relative chlorophyll contents (widely represented by Soil and Plant Analysis Development (SPAD) values) across growth stages is crucial for assessing crop health, particularly in regions characterized by varying sowing dates. Unlike previous studies focusing on high-resolution UAV imagery or specific [...] Read more.
Accurately estimating leaves’ relative chlorophyll contents (widely represented by Soil and Plant Analysis Development (SPAD) values) across growth stages is crucial for assessing crop health, particularly in regions characterized by varying sowing dates. Unlike previous studies focusing on high-resolution UAV imagery or specific growth stages, this research incorporates satellite-derived texture indices (TIs) into a SPAD value estimation model applicable across multiple growth stages (from tillering to grain-filling). Field experiments were conducted in Jiangsu Province, China, where winter wheat sowing dates varied significantly from field to field. Sentinel-2 imagery was employed to extract vegetation indices (VIs) and TIs. Following a two-step variable selection method, Random Forest (RF)-LassoCV, five machine learning algorithms were applied to develop estimation models. The newly developed model (SVR-RBFVIs+TIs) exhibited robust estimation performance (R2 = 0.8131, RMSE = 3.2333, RRMSE = 0.0710, and RPD = 2.3424) when validated against independent SPAD value datasets collected from fields with varying sowing dates. Moreover, this optimal model also exhibited a notable level of transferability at another location with different sowing times, wheat varieties, and soil types from the modeling area. In addition, this research revealed that despite the lower resolution of satellite imagery compared to UAV imagery, the incorporation of TIs significantly improved estimation accuracies compared to the sole use of VIs typical in previous studies. Full article
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