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Keywords = trees of function systems

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21 pages, 5711 KB  
Article
A Study on High-Precision Dimensional Measurement of Irregularly Shaped Carbonitrided 820CrMnTi Components
by Xiaojiao Gu, Dongyang Zheng, Jinghua Li and He Lu
Materials 2026, 19(8), 1491; https://doi.org/10.3390/ma19081491 - 8 Apr 2026
Viewed by 118
Abstract
For irregularly shaped 820CrMnTi carburizing and nitriding parts, the challenges of high reflectivity-induced overexposure, low surface contrast, and interference from minute burrs in industrial online inspection are addressed in this paper. An innovative precision detection method integrating adaptive imaging and a dual-drive heterogeneous [...] Read more.
For irregularly shaped 820CrMnTi carburizing and nitriding parts, the challenges of high reflectivity-induced overexposure, low surface contrast, and interference from minute burrs in industrial online inspection are addressed in this paper. An innovative precision detection method integrating adaptive imaging and a dual-drive heterogeneous coupling model (RGFCN) is proposed. Such parts, due to surface photovoltaic characteristic changes caused by carburizing and nitriding heat treatment and the complex on-site lighting environment, are prone to local overexposure and “false out-of-tolerance” measurements caused by outlier sensitivity in traditional inspections. First, an innovative programmatic adaptive exposure control algorithm based on grayscale histogram feedback is introduced, which dynamically adjusts imaging parameters in real time to effectively suppress high-brightness overexposure under specific working conditions. Second, a novel adaptive main-axis scanning strategy is designed to construct a dynamic follow-up coordinate system, eliminating projection errors introduced by random positioning from a geometric perspective. Additionally, Gaussian gradient energy fields are combined with the Huber M-estimation robust fitting mechanism to suppress thermal noise while automatically reducing the weight of burrs and oil stains, achieving “immunity” to non-functional defects. Meanwhile, a data-driven innovative compensation approach is introduced. Based on sample training, gradient boosting decision trees (GBDTs) are integrated to explore the nonlinear mapping relationship between multidimensional feature spaces and system residuals, achieving implicit calibration of lens distortion and environmental coupling errors. By simulating factory conditions with drastic 24 h day–night lighting fluctuations and strong oil stain interference, statistical analysis of over 1000 mass-produced parts shows that this method exhibits excellent robustness in complex environments. It reduces the false out-of-tolerance rate caused by burrs by over 90%, and the standard deviation of repeated measurements converges to the micrometer level. This effectively addresses the visual inspection challenges of irregular, highly reflective parts on dynamic production lines. Full article
(This article belongs to the Special Issue Latest Developments in Advanced Machining Technologies for Materials)
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29 pages, 4375 KB  
Article
Application of AI in Tablet Development: An Integrated Machine Learning Framework for Pre-Formulation Property Prediction
by Masugu Hamaguchi, Tomoki Adachi and Noriyoshi Arai
Pharmaceutics 2026, 18(4), 452; https://doi.org/10.3390/pharmaceutics18040452 - 8 Apr 2026
Viewed by 150
Abstract
Background/Objectives: Tablet development requires simultaneous optimization of multiple quality attributes under limited experimental budgets, yet formulation–property relationships are highly nonlinear in mixture systems. To support pre-formulation decision-making prior to extensive tablet prototyping, this study proposes an AI framework that organizes formulation and process [...] Read more.
Background/Objectives: Tablet development requires simultaneous optimization of multiple quality attributes under limited experimental budgets, yet formulation–property relationships are highly nonlinear in mixture systems. To support pre-formulation decision-making prior to extensive tablet prototyping, this study proposes an AI framework that organizes formulation and process data together with raw-material property records into a reusable database, and enriches conventional composition/process features with physically motivated mixture descriptors derived from raw-material properties and formulation/process settings. Methods: Mixture-level scalar descriptors are constructed by composition-weighted aggregation of material properties, and particle size distribution (PSD) is incorporated via a compact set of summary statistics computed from composition-weighted mixture PSDs. Three feature sets are compared: (i) Materials + Processes (MP), (ii) MP with scalar Descriptors (MPD), and (iii) MPD with PSD summaries (MPDD). Five target properties are modeled: hardness, disintegration time, flow function, cohesion, and thickness. We train and evaluate Random Forest, Extra Trees Regressor, Lasso, Partial Least Squares, Support Vector Regression, and a multi-branch neural network that processes the three feature blocks separately and concatenates them for prediction. For interpolation assessment, repeated Train/Dev/Test splitting (5:3:2) across multiple random seeds is used, and the effect of feature augmentation is quantified by paired RMSE improvements with bootstrap confidence intervals and paired Wilcoxon signed-rank tests. To assess robustness under practical formulation updates, rolling-origin time-series splits are employed and Applicability Domain indicators are computed to characterize out-of-distribution coverage. Results: Across interpolation evaluations, mixture-descriptor augmentation (MPD/MPDD) improves hardness and disintegration time in most settings, whereas gains for flow function are smaller and cohesion/thickness show mixed effects under limited sample sizes. Conclusions: Under extrapolation-oriented evaluation, the descriptors can improve hardness but may degrade disintegration-time prediction under covariate shift, emphasizing the need for careful descriptor selection and dimensionality control when deploying pre-formulation predictors. Full article
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38 pages, 3132 KB  
Article
Lightweight Semantic-Aware Route Planning on Edge Hardware for Indoor Mobile Robots: Monocular Camera–2D LiDAR Fusion with Penalty-Weighted Nav2 Route Server Replanning
by Bogdan Felician Abaza, Andrei-Alexandru Staicu and Cristian Vasile Doicin
Sensors 2026, 26(7), 2232; https://doi.org/10.3390/s26072232 - 4 Apr 2026
Viewed by 652
Abstract
The paper introduces a computationally efficient semantic-aware route planning framework for indoor mobile robots, designed for real-time execution on resource-constrained edge hardware (Raspberry Pi 5, CPU-only). The proposed architecture fuses monocular object detection with 2D LiDAR-based range estimation and integrates the resulting semantic [...] Read more.
The paper introduces a computationally efficient semantic-aware route planning framework for indoor mobile robots, designed for real-time execution on resource-constrained edge hardware (Raspberry Pi 5, CPU-only). The proposed architecture fuses monocular object detection with 2D LiDAR-based range estimation and integrates the resulting semantic annotations into the Nav2 Route Server for penalty-weighted route selection. Object localization in the map frame is achieved through the Angular Sector Fusion (ASF) pipeline, a deterministic geometric method requiring no parameter tuning. The ASF projects YOLO bounding boxes onto LiDAR angular sectors and estimates the object range using a 25th-percentile distance statistic, providing robustness to sparse returns and partial occlusions. All intrinsic and extrinsic sensor parameters are resolved at runtime via ROS 2 topic introspection and the URDF transform tree, enabling platform-agnostic deployment. Detected entities are classified according to mobility semantics (dynamic, static, and minor) and persistently encoded in a GeoJSON-based semantic map, with these annotations subsequently propagated to navigation graph edges as additive penalties and velocity constraints. Route computation is performed by the Nav2 Route Server through the minimization of a composite cost functional combining geometric path length with semantic penalties. A reactive replanning module monitors semantic cost updates during execution and triggers route invalidation and re-computation when threshold violations occur. Experimental evaluation over 115 navigation segments (legs) on three heterogeneous robotic platforms (two single-board RPi5 configurations and one dual-board setup with inference offloading) yielded an overall success rate of 97% (baseline: 100%, adaptive: 94%), with 42 replanning events observed in 57% of adaptive trials. Navigation time distributions exhibited statistically significant departures from normality (Shapiro–Wilk, p < 0.005). While central tendency differences between the baseline and adaptive modes were not significant (Mann–Whitney U, p = 0.157), the adaptive planner reduced temporal variance substantially (σ = 11.0 s vs. 31.1 s; Levene’s test W = 3.14, p = 0.082), primarily by mitigating AMCL recovery-induced outliers. On-device YOLO26n inference, executed via the NCNN backend, achieved 5.5 ± 0.7 FPS (167 ± 21 ms latency), and distributed inference reduced the average system CPU load from 85% to 48%. The study further reports deployment-level observations relevant to the Nav2 ecosystem, including GeoJSON metadata persistence constraints, graph discontinuity (“path-gap”) artifacts, and practical Route Server configuration patterns for semantic cost integration. Full article
(This article belongs to the Special Issue Advances in Sensing, Control and Path Planning for Robotic Systems)
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24 pages, 2237 KB  
Article
Binary Logistic Regression Outperforms Decision Tree Modeling for Event-Based Landslide Prediction: Application to Dynamic Hazard and Threshold Mapping in Central Italy
by Matteo Gentilucci, Hamed Younes, Rihab Hadji and Gilberto Pambianchi
Earth 2026, 7(2), 56; https://doi.org/10.3390/earth7020056 - 31 Mar 2026
Viewed by 262
Abstract
The increasing frequency of disasters caused by landslides, mainly due to climate change leading to more intense extreme events, requires reliable predictive models for risk mitigation. Italy, in particular, is a country at high risk of landslides, but the lack of an updated [...] Read more.
The increasing frequency of disasters caused by landslides, mainly due to climate change leading to more intense extreme events, requires reliable predictive models for risk mitigation. Italy, in particular, is a country at high risk of landslides, but the lack of an updated catalogue of landslide activation dates poses a significant challenge for defining reliable activation thresholds. This study develops a methodology for mapping landslide susceptibility based on events in a pilot area of central Italy, integrating a database of landslides with known activation dates with predisposing and triggering parameters. Two statistical techniques were compared to assess their predictive performance in discriminating landslide from non-landslide conditions during extreme precipitation events. A comparison between binary logistic regression (BLR) and decision trees (QUEST) revealed the clear superiority of the BLR model, which achieved excellent predictive accuracy (AUC = 0.913). The model identified clay-rich lithology, gentle slopes (0–16°) and maximum daily precipitation as the most significant controlling factors. This result led to the generation of three derivative products: a susceptibility map, a hazard map for an extreme precipitation scenario with a 100-year return period, and a spatially distributed map of activation thresholds. This threshold map quantifies the intensity of precipitation required to exceed a critical probability of landslide initiation (p > 0.7) at any point in the territory. The susceptibility map highlights critical areas within the study area, while the hazard map also includes the return period of the event. The threshold map is a direct and operational tool for early warning systems, transforming a statistical model into a guide for real-time risk management. The study area serves as a pilot area that could allow this methodology to be replicated. With the integration of real-time meteorological data, it could function as a real-time warning system. The proposed framework therefore provides a directly actionable tool for civil protection agencies, land-use planning authorities, and emergency managers, enabling location-specific rainfall alert thresholds to be issued rather than a single regional value, with the potential to reduce both false alarms and missed warnings. Full article
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24 pages, 3168 KB  
Article
Application of Machine Learning Models to Oil Refinery Programming
by Evar Umeozor
Processes 2026, 14(7), 1072; https://doi.org/10.3390/pr14071072 - 27 Mar 2026
Viewed by 358
Abstract
Transparent and evidence-based representations of global crude oil refining systems remain limited in the public literature, constraining robust energy systems modeling and policy analysis. This study develops a comprehensive, configuration-based modeling framework for all operating crude oil refineries worldwide using plant-level process unit [...] Read more.
Transparent and evidence-based representations of global crude oil refining systems remain limited in the public literature, constraining robust energy systems modeling and policy analysis. This study develops a comprehensive, configuration-based modeling framework for all operating crude oil refineries worldwide using plant-level process unit data. Forty unique refinery configurations are identified through an unsupervised decision tree-based clustering approach that accounts for process unit presence and relative conversion intensity. An extremely randomized trees (ETR) machine learning model is trained on approximately 11,000 refinery-year observations to predict refined product yields as a function of refinery configuration, capacity, and crude oil diet. The model achieves out-of-sample coefficients of determination exceeding 0.90 for all major products and outperforms multiple linear regression and other ensemble methods. The predictive model is integrated with a differential evolution optimization algorithm to enable refinery programming under operational and feedstock constraints. The application of this model to Gulf Cooperation Council (GCC) refineries shows that, under existing technologies, petrochemical feedstock yields are bounded at approximately 37%, significantly below announced long-term diversification targets of 70–85%. Yield improvements of up to 6 percentage points are feasible through operational optimization but are associated with capacity utilization adjustments and product trade-offs. The framework provides a scalable tool for refinery benchmarking, energy transition analysis, and strategic planning across facility, national, and global levels. Full article
(This article belongs to the Special Issue Feature Review Papers in Section "Chemical Processes and Systems")
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30 pages, 3840 KB  
Article
Enhancing Asset Management: Deterioration and Seismic-Based Decision-Support Framework for Heterogeneous Portfolios
by Marco Gaspari, Margherita Fabris, Luca Tosolini, Elisa Saler, Marco Donà and Francesca da Porto
Buildings 2026, 16(7), 1293; https://doi.org/10.3390/buildings16071293 - 25 Mar 2026
Viewed by 223
Abstract
The management of large and heterogeneous building stocks requires decision-support tools capable of prioritising interventions under limited technical and financial resources. In this framework, the role of structural deterioration is rarely integrated within a unified prioritisation framework. This study proposes a rapid deterioration-based [...] Read more.
The management of large and heterogeneous building stocks requires decision-support tools capable of prioritising interventions under limited technical and financial resources. In this framework, the role of structural deterioration is rarely integrated within a unified prioritisation framework. This study proposes a rapid deterioration-based assessment for prioritising maintenance within heterogenous portfolios. The assessment is articulated into two levels. A Project Level (PL) is based on visual inspections and component-level condition ratings, while a Network Level (NL) introduces contextual and functional modifiers related to the relevance of each structural unit within the building stock. A seismic assessment procedure is integrated in proposed decision-making system for optimising intervention planning. The two assessments are integrated through a decision-tree logic providing an overall classification of buildings within portfolios. The proposed framework is applied to an industrial-oriented building stock located in Italy, comprising 79 structural units characterised by significant typological heterogeneity, including masonry, reinforced concrete, precast reinforced concrete, and steel buildings. The application illustrates the internal consistency of the proposed framework and its ability to support a transparent and articulated prioritisation process for maintenance and risk mitigation within heterogeneous building portfolios. Further applications to different building stocks are required to explore the general applicability of the methodology. Full article
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42 pages, 2531 KB  
Article
Design Principles for a New Form of Bioelectrical Nanonetwork Based on Cellular Nanowires
by Konstantinos F. Kantelis, Vassilis Asteriou, Aliki Papadimitriou-Tsantarliotou, Olga Tsave, Christos Liaskos, Christos A. Ouzounis, Lefteris Angelis, Ioannis S. Vizirianakis, Petros Nicopolitidis and Georgios I. Papadimitriou
J. Sens. Actuator Netw. 2026, 15(2), 30; https://doi.org/10.3390/jsan15020030 - 23 Mar 2026
Viewed by 521
Abstract
Nanotechnology continues to advance rapidly, revealing previously unexplored directions in nanoscale communications. Biological and electromagnetic nanonetworks—established communication paradigms at the nanoscale—have shifted interest toward the middle and higher levels of the nanonetworking protocol stack. Motivated by the discovery of Cable Bacteria (CB) and [...] Read more.
Nanotechnology continues to advance rapidly, revealing previously unexplored directions in nanoscale communications. Biological and electromagnetic nanonetworks—established communication paradigms at the nanoscale—have shifted interest toward the middle and higher levels of the nanonetworking protocol stack. Motivated by the discovery of Cable Bacteria (CB) and their unique properties, we propose a theoretical model and framework for a new category of nanonetworks: bioelectrical nanonetworks (BioEN). This proposed framework combines the biocompatibility, sustainability and inherent nanodimensions of biological organisms with the networking performance of electromagnetic systems. Large-scale formations (e.g., 10,000 cells spanning nearly 2 cm), together with the electrical characteristics of CB, suggest the feasibility of guided electron-based transport that could complement diffusion-dominated nanonetworks, subject to resistive-capacitive (RC) constraints that remain to be quantified. Furthermore, we present a set of basic network architectures—such as star, ring, and tree—introducing a conceptual bio-multiplexer component, which utilizes CB to form a bioelectrical nanonetwork and illustrate core functionalities primarily at the network layer. Within this theoretical framework, BioEN is positioned as a potential enabler for diverse scientific, environmental, and technological applications, including health and ecosystem biosensing and bioremediation-oriented bioengineering. This work is conceptual and does not experimentally validate a deployed nanonetwork; instead, it establishes the design principles, abstractions, and architectural foundations intended to guide future implementation and experimental verification of bioelectrical nanonetworks. Full article
(This article belongs to the Section Communications and Networking)
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28 pages, 610 KB  
Article
Exploring the Feasibility of Fall Detection Using Bluetooth Low Energy Channel Sounding in Residential Environments
by Šarūnas Paulikas and Simona Paulikiene
Sensors 2026, 26(6), 1930; https://doi.org/10.3390/s26061930 - 19 Mar 2026
Viewed by 364
Abstract
Falls represent a major health risk for older adults living independently, motivating the development of unobtrusive and privacy-preserving monitoring solutions. This study investigates whether Bluetooth Low Energy (BLE) 6.0 Channel Sounding (CS) can support device-free fall detection using low-complexity signal representations suitable for [...] Read more.
Falls represent a major health risk for older adults living independently, motivating the development of unobtrusive and privacy-preserving monitoring solutions. This study investigates whether Bluetooth Low Energy (BLE) 6.0 Channel Sounding (CS) can support device-free fall detection using low-complexity signal representations suitable for residential deployment. The proposed system employs two BLE nodes performing periodic channel sounding, from which only scalar distance estimates are extracted. Time-domain and temporal-dynamic features are computed from sliding windows of the distance signal and used for supervised classification. Three widely used classifiers—Support Vector Machine with radial basis function kernel, Random Forest, and gradient-boosted decision trees (XGBoost)—are evaluated under both a default operating point and a sensitivity-first regime achieved through validation-based decision threshold adjustment, reflecting the higher cost of missed fall detections in assisted living scenarios. Experiments conducted in a furnished indoor environment with six participants performing realistic fall and non-fall scenarios demonstrate strong window-level sensitivity under subject-independent evaluation, with XGBoost providing the most favorable sensitivity–specificity balance. Under sensitivity-first operation, very high recall is achieved at the expense of increased false alarms. Given the limited dataset and single-environment setting, the reported results should be interpreted as a proof-of-concept demonstration of feasibility rather than definitive large-scale performance. The findings suggest that BLE CS captures motion-relevant signal variations that may support practical fall detection while maintaining low deployment complexity and privacy preservation. Full article
(This article belongs to the Section Electronic Sensors)
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18 pages, 1505 KB  
Article
Exploratory Study of the Correlation Between the Vegetative Growth of Olive Trees (Olea europaea L.), the Quality Characteristics of Olive Oil and Sensory Properties in Algerian and European Cultivars
by Nadjya Chalabi, Fayçal Bahlouli and Agustí J. Romero-Aroca
Agronomy 2026, 16(6), 616; https://doi.org/10.3390/agronomy16060616 - 13 Mar 2026
Viewed by 419
Abstract
Olive tree cultivation occupies a central place in Algerian agriculture and is of considerable economic and cultural importance. Several production factors strongly influence the quality of olive oil. Among the determinants of this quality, the vegetative growth of the olive tree plays a [...] Read more.
Olive tree cultivation occupies a central place in Algerian agriculture and is of considerable economic and cultural importance. Several production factors strongly influence the quality of olive oil. Among the determinants of this quality, the vegetative growth of the olive tree plays a crucial role, as it controls photosynthetic capacity, the distribution of assimilates, and fruit filling. These physiological mechanisms directly influence oil percentage, as well as fatty acid and phenolic compound compositions, and consequently, sensory characteristics such as bitterness and pungency. This study examines the quantitative relationships between vegetative growth, chemical parameters, and sensory attribute interactions that are still poorly understood using seven representative olive cultivars: local varieties (Chemlal, Bouchouk Lafayette, Blanquette de Guelma, Sigoise, and Limli) and European varieties (Frantoio and Belgentéroise). Vegetative growth was characterized by the average shoot length; fruit oil content was expressed as a percentage on a dry basis, and fatty acids were analyzed by gas chromatography after derivatization. The total polyphenol content was determined by spectrophotometry and expressed as concentration, and oxidative stability was measured using the Rancimat method. Sensory analysis was conducted by a trained panel in accordance with international recommendations. The results indicate substantial positive correlations between vegetative growth parameters, oil concentration, olive oil composition, and those sensory attributes related to polyphenols, for all varieties studied. This functional consistency suggests that improvement in one parameter is generally associated with improvement in others. The Algerian variety Chemlal stands out for its optimal performance profile in agronomic, chemical, and sensory aspects compared to the other varieties. These preliminary results suggest that optimizing oil characteristics is directly linked to the physiological and biochemical performance of the olive tree, thus confirming the relevance of a systems approach in the selection and management of olive varieties. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
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18 pages, 6046 KB  
Article
Transcriptome of lncRNAs and mRNAs and Their Network Profile in Relation to Phenotypic Variation in Grafted Peach–Apricot Chimeras
by Jiajia Chen, Bingxin Fan, Xiaokui Hou, Shixing Wang, Zhaokun Zhi, Huafeng Yue, Shulin Zhang, Gaopu Zhu and Mengmeng Zhang
Horticulturae 2026, 12(3), 345; https://doi.org/10.3390/horticulturae12030345 - 12 Mar 2026
Viewed by 253
Abstract
Grafted plants carrying DNA from both species are prone to new phenotypes. Specific long non-coding RNA (lncRNA) sequences are known to play roles in the formation and development of grafted plants. However, the roles of lncRNAs in phenotypic variation in grafts between peach [...] Read more.
Grafted plants carrying DNA from both species are prone to new phenotypes. Specific long non-coding RNA (lncRNA) sequences are known to play roles in the formation and development of grafted plants. However, the roles of lncRNAs in phenotypic variation in grafts between peach and apricot remain unexplored. In this study, mixed tissues (leaves, buds and fully bloomed flowers) of peach branches from heterografts between apricot/peach (A/P) and peach/apricot (P/A) and homografted peach (SP) were collected for transcriptome sequencing. The differentially expressed genes (DEGs) and lncRNAs (DElncRNAs) between A/P and P/A were identified as candidates mediating the formation of divergent traits. Compared with SP, 1115 and 624 DEGs were detected in A/P and P/A, respectively. There were 173 DEGs shared between A/P and P/A, whereas the transcripts of 942 genes were specifically altered in A/P and 451 DEGs were specific to P/A. There were 29 DElncRNAs in A/P and 26 DElncRNAs in P/A, of which, 21 DElncRNAs were specific to A/P and 18 were specific to P/A. The biological functions of the DEGs and DElncRNAs were predicted via GO and KEGG enrichment analyses. A total of 24 co-expressed ‘lncRNA-mRNA’ pairs were identified, including 14 ‘lncRNA-mRNA’ pairs in A/P and 10 ‘lncRNA-mRNA’ pairs in P/A. The ‘MSTRG.17020.2-XM_007210198-2’ pair potentially participates in aminoacyl biosynthesis, and the ‘MSTRG.8395.1-XM_007217967.2’ pair may regulate galactose metabolism. The lncRNA MSTRG.6365.3 may regulate defense response through altering the levels of XM_020556240.1 and XM_020556234.1. These findings provide valuable insights into the molecular mechanisms underlying grafting-induced differential trait formation and establish a foundation for further research on the functional roles of ‘lncRNA-mRNA’ pairs in fruit tree grafting systems. Full article
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26 pages, 5511 KB  
Article
Adapting Mediterranean Agroforestry to Global Change: Trade-Offs and Lessons from the Montado
by Nour-Elhouda Fatahi, Teresa Pinto-Correia, Maria de Belém Costa Freitas, João Tiago Marques and Hatem Belhouchette
Sustainability 2026, 18(6), 2725; https://doi.org/10.3390/su18062725 - 11 Mar 2026
Viewed by 418
Abstract
The Montado, a traditional Mediterranean agro-silvopastoral system, has historically sustained ecological and economic functions through the integration of trees, livestock, and crops. Today, its multifunctionality is increasingly threatened by climate variability, market volatility, and evolving policy frameworks. While previous research has examined Montado [...] Read more.
The Montado, a traditional Mediterranean agro-silvopastoral system, has historically sustained ecological and economic functions through the integration of trees, livestock, and crops. Today, its multifunctionality is increasingly threatened by climate variability, market volatility, and evolving policy frameworks. While previous research has examined Montado dynamics at landscape or plot scales, less attention has been paid to sustainability trajectories at the farm level, where management decisions are made. This study bridges that gap by assessing the sustainability dynamics of farms through a participatory, typology based, scenario approach grounded in a regional typology. We characterized three representative farm archetypes (forestry-focused, mixed agro-silvopastoral, and livestock-focused) and evaluated their trajectories under plausible future scenarios driven by climate, market, and policy pressures. Scenario outcomes were assessed using expert-based scoring (five-point scale), revealing score differences of up to two points across sustainability dimensions between farm archetypes and scenarios. Findings reveal marked trade-offs: Tree-focused farms maintain high environmental value but remain vulnerable to market and labor constraints, while livestock-specialized farms achieve higher economic output at the expense of ecological integrity. Mixed systems demonstrate greater resilience through diversification but face significant labor intensity challenges. We conclude that current “one-size-fits-all” policies generate contradictory incentives. Therefore, adaptive governance frameworks (e.g., results-based payment schemes) are essential to realign farm economics with ecological stewardship. Beyond the Montado, the approach provides insights relevant to other Mediterranean agroforestry systems facing similar sustainability challenges. Full article
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16 pages, 14979 KB  
Article
A Fruit-Pulp-Derived Callus-Level Agrobacterium-Mediated Transformation Platform for Ziziphus jujuba
by Junyu Song, Zhong Zhang, Jingnan Shi, Kexin Wei, Peilin Han, Zhongwu Wan and Xingang Li
Plants 2026, 15(5), 843; https://doi.org/10.3390/plants15050843 - 9 Mar 2026
Viewed by 446
Abstract
The jujube (Ziziphus jujuba Mill.) is a significant economic fruit tree, valued for its nutritional and medicinal properties. However, advances in functional genomics are hindered by the lack of an efficient transformation system. To overcome the limitations of conventional explant, we established [...] Read more.
The jujube (Ziziphus jujuba Mill.) is a significant economic fruit tree, valued for its nutritional and medicinal properties. However, advances in functional genomics are hindered by the lack of an efficient transformation system. To overcome the limitations of conventional explant, we established a fruit-pulp-derived, callus-based Agrobacterium-mediated transformation system using fruit-pulp harvested 50 days after pollination. Through orthogonal experimental design, 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid were identified as key regulators for inducing high-quality, friable callus in two jujube genotypes, ‘JZ60’ and ‘LWCZ’. This system revealed significant genotype-specific variation in auxin requirements for callus proliferation and in differential antibiotic sensitivity. Transformation efficiency, as evaluated by fluorescence screening, was primarily determined by acetosyringone concentration and the binary vector architecture. The results revealed that the compact pCY (kanamycin resistance) vector achieved higher transformation efficiency (up to 77.8%) than pCAMBIA1301, whereas the pCAMBIA1301 (hygromycin resistance) vector enabled more uniform transgene expression. Integration and expression of the ZjCBF3 transgene were confirmed by polymerase chain reaction (PCR), reverse transcription quantitative PCR, and green fluorescent protein fluorescence assays. This study established a fruit-pulp-based callus transformation system for jujube, providing a rapid platform for its functional genomic studies. Full article
(This article belongs to the Special Issue Advances in Jujube Research, Second Edition)
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21 pages, 1285 KB  
Article
Nonlinear Feature-Based MI Detection Supported by DWT and EMD on ECG: A High-Performance Decision Support Approach
by Ali Narin and Merve Keser
Biosensors 2026, 16(3), 150; https://doi.org/10.3390/bios16030150 - 4 Mar 2026
Viewed by 490
Abstract
Myocardial infarction (MI) is a life-threatening cardiovascular disorder caused by a partial or complete interruption of oxygenated blood flow to the myocardium, leading to high mortality rates if not diagnosed promptly. Although electrocardiogram (ECG) signals are widely used due to their non-invasive and [...] Read more.
Myocardial infarction (MI) is a life-threatening cardiovascular disorder caused by a partial or complete interruption of oxygenated blood flow to the myocardium, leading to high mortality rates if not diagnosed promptly. Although electrocardiogram (ECG) signals are widely used due to their non-invasive and low-cost nature, MI-specific abnormalities may be subtle and subject to inter-observer variability. Therefore, reliable artificial intelligence-based decision support systems are essential to enhance diagnostic classification accuracy. In this study, only the Lead II derivation from 12-lead ECG recordings of 52 healthy individuals and 148 MI patients was analyzed. To effectively characterize the non-stationary nature of ECG signals, a hybrid time–frequency feature extraction framework was employed. Five-level intrinsic mode functions and wavelet detail and approximation coefficients were obtained using Empirical Mode Decomposition and Discrete Wavelet Transform with a Daubechies-6 wavelet. From these components, 390 times, nonlinear and complexity-based features were extracted using 23 entropy-driven measures. Particle Swarm Optimization was applied to select the most discriminative feature subset, significantly enhancing classification performance. The optimized features were evaluated using Support Vector Machines, Artificial Neural Networks, k-Nearest Neighbors, and Bagged Tree classifiers. The Bagged Trees classifier achieved the best classification performance with an overall correct classification rate of 97.6%. The results demonstrate that the proposed hybrid feature representation combined with PSO-based selection provides a robust and reliable framework for MI detection, offering strong potential for clinical decision support applications. Full article
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23 pages, 6566 KB  
Article
Biocultural Productive Landscapes in the Andean–Amazon: Carbon, Biodiversity, and Livelihoods in Market-Linked Traditional Systems
by Bolier Torres, Cristhian Tipán-Torres, Héctor Reyes, Aracely Tapia, Julio Muñoz-Rengifo, Robinson Herrera-Feijoo and Antón García
Sustainability 2026, 18(5), 2451; https://doi.org/10.3390/su18052451 - 3 Mar 2026
Viewed by 387
Abstract
Tree-based production systems embedded within Amazonian biocultural landscapes remain systematically undervalued in global climate, biodiversity, and development policy frameworks. This study assessed tree diversity, structural attributes, and carbon stocks across traditional cacao-based Amazonian agroforestry systems (Chakra), tree-rich silvopastoral systems, and old-growth forests in [...] Read more.
Tree-based production systems embedded within Amazonian biocultural landscapes remain systematically undervalued in global climate, biodiversity, and development policy frameworks. This study assessed tree diversity, structural attributes, and carbon stocks across traditional cacao-based Amazonian agroforestry systems (Chakra), tree-rich silvopastoral systems, and old-growth forests in the Andean–Amazon transition zone of Ecuador. Based on 28 sampling plots (DBH ≥ 10 cm), old-growth forests stored the highest aboveground carbon stocks, while agroforestry and silvopastoral systems retained approximately 20–30% of forest carbon, equivalent to ~100–180 Mg CO2-equivalent ha−1—far exceeding values reported for monocultures or treeless pastures. A total of 151 tree species were recorded across all land-use systems, with forests harboring the highest richness (122 species), followed by agroforestry (35 species) and silvopastoral systems (28 species). Carbon storage was highly concentrated in a limited subset of multifunctional species: in agroforestry systems, eight species accounted for ~80% of total aboveground CO2-equivalent stocks, whereas in silvopastoral systems only five species explained a similar proportion. Dominant taxa such as Cordia alliodora, Inga edulis, Jacaranda copaia, Piptocoma discolor, and Piptadenia pteroclada illustrate a process of biocultural species filtering, whereby trees providing food, timber, shade, and cultural value are selectively retained while sustaining significant carbon stocks. These findings demonstrate that tree-based productive systems function as biocultural productive landscapes that conserve carbon, biodiversity, and livelihoods beyond forest boundaries. We argue for their formal inclusion, particularly traditional silvopastoral systems, within climate finance mechanisms, nationally determined contributions (NDCs), and biocultural heritage frameworks, alongside forest conservation strategies. Full article
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Article
A Stochastic Simulation Framework to Predict the Spatial Spread of Xylella fastidiosa
by Nikolaos Marios Polymenakos, Iosif Polenakis, Christos Sarantidis, Ioannis Karydis and Markos Avlonitis
Mathematics 2026, 14(5), 847; https://doi.org/10.3390/math14050847 - 2 Mar 2026
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Abstract
The spread of Xylella fastidiosa, a xylem-limited bacterial pathogen, has caused widespread mortality among olive trees in Apulian region, Italy in more than a decade, and represents a significant threat to Mediterranean agroecosystems. To encourage evidence-based containment strategies, we developed a stochastic, [...] Read more.
The spread of Xylella fastidiosa, a xylem-limited bacterial pathogen, has caused widespread mortality among olive trees in Apulian region, Italy in more than a decade, and represents a significant threat to Mediterranean agroecosystems. To encourage evidence-based containment strategies, we developed a stochastic, spatiotemporal simulation model that represents pathogen transmission at the individual-tree level. This work integrates high-resolution georeferenced olive-tree data and implicitly incorporates vector population dynamics through a tree-specific vulnerability index, which considers local host density and landscape connectivity. Vector dispersal is approximated using a radial transmission kernel, which preserves host–vector spatial interactions while avoiding the explicit modeling of insect trajectories. The system’s spatial structure is additionally formulated as a proximity graph, facilitating network-based analysis of spread pathways. A series of Monte Carlo simulation experiments is employed for calibration against the observed epidemic footprint, while validation utilizes independent infection records and global sensitivity analysis of key parameters. The findings indicate that the model effectively replicates realistic propagation patterns, and its calibrated parameters are consistent with out-of-sample data. This makes it an appropriate exploratory tool for scenario testing, assessing the potential impact of intervention strategies, and offering risk-based decision support for handling Xylella fastidiosa outbreaks. Subsequently, graph centrality metrics are used to identify epidemiologically critical trees that function as transmission bridges, thus representing priority targets for surveillance or removal efforts. Thus, multiple tests have been conducted using betweenness and closeness centrality, while comparing both methods leads to effective node-tree removal decisions. Full article
(This article belongs to the Special Issue Nonlinear Dynamics and Stochastic Modeling of Complex Systems)
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