Search Results (2,339)

The genus Pseudobagrus represents a group of economically and ecologically significant freshwater bagrid catfishes in East Asia, yet its taxonomy remains contentious. This study employed the complete mitochondrial genomes of 15 Pseudobagrus species to clarify their phylogenetic relationships. The mitogenomes ranged from 16,526 to 16,647 bp, exhibiting a conserved gene order and significant AT bias (average A + T = 57.85%). All genomes contained 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and a control region. Start codons were predominantly ATG, except for COI (GTG), while stop codons varied among TAA, TAG, and incomplete T--. Codon usage bias favored NNU and NNA codons, and 12 optimal codons were identified in P. albomarginatus. The phylogenetic trees based on concatenated PCGs revealed two major clades. Clade I contained 14 species. Within this clade, P. albomarginatus, P. tenuis, and P. brevicorpus clustered together first, and then this trio grouped with P. ussuriensis. Pseudobagrus trilineatus formed the separate Clade II. These results provide a molecular foundation for species delimitation and systematic revision within Pseudobagrus, supporting the monophyly of the genus while highlighting cryptic diversity and taxonomic complexity. Full article

Seed endophytic microbiota are crucial for plant early development and stress resistance. Pinus massoniana is a key ecological and economic tree species in China, yet it is severely threatened by pine wilt disease (PWD). However, the community composition of P. massoniana seed endophytic microbiota and the persistent symbiosis formed via vertical transmission in seeds remain unclear. We analyzed the endophytic bacterial and fungal microbiota of P. massoniana seeds from four geographic regions using high-throughput 16S rRNA and ITS sequencing to characterize community structure, diversity, and functional potential, providing a basis for endophytic microbiota-based strategies to enhance resistance to PWD. Results showed that both alpha and beta diversity analyses indicated that seed endophytic microbial communities of P. massoniana differed among regions. Bacterial communities were dominated by Pseudomonadota (phylum), Gammaproteobacteria (class), and the genera Klebsiella, norank_f_Pectobacteriaceae, and Lactobacillus. Fungal communities were primarily composed of Ascomycota and Basidiomycota (phylum), Sordariomycetes (class), and the genera Rosellinia, Aspergillus, and Coniophora. Correlation network analysis revealed that fungal networks were characterized by a higher proportion of positive correlations, whereas bacterial networks were more complex. Notably, several genera detected in seeds, including Pseudomonas, Bacillus, and Trichoderma, have also been reported in mature P. massoniana tissues, indicating a potential for putative vertical transmission from mother plants. Functional prediction further suggested that these taxa were enriched in pathways related to terpenoid and polyketide metabolism and saprotrophic functions, which have been implicated in PWD resistance and have been previously reported to exert nematode-suppressive or plant growth-promoting effects. Overall, this study elucidates the community structure and ecological characteristics of seed endophytic microbiota in P. massoniana and identifies potentially beneficial microbial taxa, providing potential support for the future utilization of P. massoniana endophytic microbiota in PWD research. Full article

The medicinally and ornamentally valuable genus Thunbergia faces taxonomic uncertainty, while certain Acanthaceae species are threatened by habitat loss, underscoring the need for chloroplast genome studies to support conservation efforts. The chloroplast genome of Thunbergia grandiflora was sequenced and assembled. Additionally, 28 Acanthaceae species with significant medicinal value were selected for comparative genomic analysis. Based on the chloroplast genome data of Acanthaceae species, this study conducted phylogenetic and comparative evolutionary analyses. The results preliminarily support a systematic framework that divides Acanthaceae into eight tribes within five subfamilies. Concurrently, the study revealed significant inverted repeat (IR) region structural variations. A clear correspondence was observed between the contraction of IR length and the topological structure of the phylogenetic tree. In particular, species within the genus Strobilanthes exhibited significant contraction in their IR regions, which corresponded consistently with their tendency to cluster into an independent clade in the phylogenetic tree. This suggests that structural variation in the IR regions may be closely associated with the evolutionary divergence of this group. SSR analysis revealed a prevalent mononucleotide A/T repeat dominant pattern across Acanthaceae species. Furthermore, selection pressure analysis detected positive selection acting on multiple key genes, including rbcL, rps3, rps12, cemA, and ycf4, suggesting that these genes may play important roles in the adaptive evolution of Acanthaceae. This study reveals that the chloroplast genomes of Acanthaceae exhibit distinctive characteristics in phylogenetic architecture, dynamic variations in IR regions, and adaptive evolution of key genes, providing important molecular insights for understanding the mechanisms underlying species diversity and for the conservation of medicinal resources within this family. Full article

Parasitoids are key natural antagonists of forest insect pests and are gaining importance in integrated forest protection under increasing climate-related disturbances. This study aimed to quantify the influence of vegetation diversity and canopy structure on the abundance and diversity of the overall insect community responses to vegetation structure and to provide an ecological context. Second, detailed analyses focused on three focal parasitoid families (Braconidae, Ichneumonidae, Tachinidae), which are of particular relevance for integrated forest protection due to their central role in integrated forest protection and in pesticide-free regulation approaches for risk mitigation in forest ecosystems. Malaise traps were deployed at eight randomly selected broadleaf and coniferous sites, and insect samples from six sampling dates in summer 2024 were analyzed. The sampling period coincided with the full development of woody and vascular plants, representing the phase of highest expected activity of phytophagous insects and associated parasitoids. Vegetation surveys (Braun–Blanquet), canopy closure, and canopy cover were recorded for each site. Across all samples, five arthropod classes, 13 insect orders, and 31 hymenopteran families were identified, with pronounced site-specific differences in community composition and abundance. Our results suggest that broadleaf-dominated sites, characterized by higher plant species richness and greater structural heterogeneity, support a more diverse assemblage of phytophagous insects, thereby increasing host availability and niche diversity for parasitoids. Parasitoid communities generally showed higher diversity at broadleaf sites. Spearman correlations and multiple linear regressions revealed a strong negative relationship between canopy cover and total insect abundance ρ (Spearman’s rank correlation coefficient (Spearman ρ = −0.72, p = 0.042; p = 0.012, R² = 0.70), R² (coefficient of determination), whereas parasitoid diversity (Shannon index) and the relative proportion of Ichneumonidae were positively associated with canopy cover (ρ = 0.85, p = 0.008). In addition, canopy cover had a significant positive effect on overall insect diversity (Shannon index; p = 0.015, R² = 0.63). Time-series analyses revealed a significant seasonal decline in parasitoid abundance (p < 0.001) and parasitoid diversity (p = 0.018). Time-series analyses revealed seasonal dynamics characterized by fluctuations in parasitoid abundance and diversity and a general decrease over the course of the sampling period. The findings demonstrate that structurally diverse mixed forests, particularly those with a high proportion of broadleaf trees mixed forests with heterogeneous canopy layers can enhance the diversity of specialized natural enemies, while dense canopy cover reduces overall insect abundance. These insights provide an ecological basis for silvicultural strategies that strengthen natural regulation processes within integrated forest protection. Full article

Eucommia ulmoides Oliv. (E. ulmoides), an endemic tree species in China, holds significant value in traditional Chinese medicine industry and health food. The plant is rich in diverse bioactive compounds, including lignans, iridoids, flavonoids, polysaccharides, E. ulmoides gum, amino acids, and minerals. These components contribute to a range of pharmacological activities such as anti-inflammatory, antioxidant, antihypertensive, immunomodulatory, and bone-protective effects, which support its long-standing traditional use and emerging clinical and adjunctive applications. While current research has predominantly focused on the bark and leaves, other parts, such as flowers, seeds, stems and roots, remain underexplored despite their substantial potential for medicinal and edible applications. Based on the recent literature, this paper systematically summarized the chemical composition, health benefits, and comprehensive utilization of different parts of E. ulmoides (bark, leaves, flowers, and seeds), aiming to provide a theoretical foundation for the high-value utilization of the entire plant resources of E. ulmoides. As a health-promoting plant resource, E. ulmoides has extensive development potential in applications such as health foods, natural medicines, and agricultural inputs. Future research should prioritize elucidating the synergistic mechanisms among different active compounds, advancing technologies for multi-part utilization, and establishing standardized quality evaluation systems to facilitate broader applications in functional foods, pharmaceuticals, and related interdisciplinary fields. Full article

This study investigated the composition and temporal dynamics of wood-inhabiting fungal communities in four aging tree species in Lednice Castle Park (Czech Republic), located within the Lednice–Valtice Cultural Landscape, a UNESCO World Heritage Site. Forty wood cores were collected from 20 trees at two time points (2023 and 2024). The hosts included horse chestnut (Aesculus hippocastanum L.), copper beech (Fagus sylvatica ‘Atropunicea’ L.), oak (Quercus robur L.), and poplar (Populus alba L.), each exhibiting visual signs of decline. Fungal assemblages were profiled using ITS2 high-throughput amplicon sequencing. Ascomycota dominated across all hosts (72–89% of reads), while Basidiomycota contributed 8–24%, largely represented by Agaricomycetes in F. sylvatica. Alpha diversity varied significantly among hosts (Shannon: F_3,36 = 10.61, p = 0.001 in 2023; F_3,36 = 10.00, p = 0.001 in 2024). Temporal shifts were host-dependent: F. sylvatica exhibited the strongest year-to-year decline in richness (Chao1: −83%, p = 0.007) and increased beta dispersion, while A. hippocastanum and P. alba showed significant increases in diversity (+65% and +42%, respectively). Community composition was shaped by host species (PERMANOVA Bray–Curtis: p = 0.001) and shifted over time (Jaccard: p = 0.001), with F. sylvatica showing the highest temporal turnover. Functional guild analysis revealed consistent dominance of saprotrophs (29–41%) and mixed pathotroph–saprotroph guilds (23–36%) across hosts, indicating active degradation processes inside functional xylem. These results indicate that, within the studied system, the wood mycobiome of aging trees is host-dependent and temporally dynamic rather than static or functionally neutral. Short-term temporal turnover observed between sampling years may contribute to shifts in fungal community composition and succession within wood, with potential implications for tree decline processes in managed historical park landscapes. Full article

In northwestern Ecuador, where more than 90% of the original forest cover has been lost, it is unknown how soil chemistry influences bat diversity. This study evaluated bat diversity, non-herbaceous plant community structure, and soil nutrients in 30 plots distributed across crops on two farms separated by 32 km. Soil analyses revealed variations in organic matter and nutrients, identifying calcium, magnesium, zinc and iron as the most influential. A total of 1662 individuals of 24 non-herbaceous plant species and 193 individuals of 16 bat species were recorded, dominated by frugivorous and nectarivorous guilds. Generalized linear mixed models showed significant relationships between bat diversity indices and soil nutrients. These elements improve tree growth, fruiting, and flowering, which increases the quality and availability of food resources for bats. In return, these mammals provide key ecosystem services such as pollination, seed dispersal, and insect control. Our findings highlight that soil chemistry indirectly regulates bat communities by influencing vegetation structure and resource availability. This integrated approach underscores the importance of soil–plant–animal interactions in tropical agricultural landscapes, offering practical guidance. Full article

We surveyed bee communities across an organic conifer tree farm landscape in northern Idaho to assess how managed forest–agriculture mosaics support pollinator diversity. Bees were collected from farm fields, adjacent conservation forests, and a pollinator garden between May and August 2024 using aerial nets and identified to species or morphospecies. In total, 94 bee species were recorded, representing a mix of ground-nesting (46%), cavity-nesting (37%), and social (17%) taxa. Bee richness was highest in farm fields (66 species), intermediate in forests (48 species), and lowest in the pollinator garden (35 species). Community turnover among habitats was substantial (Jaccard dissimilarity = 0.67–0.76; Bray-Curtis dissimilarity = 0.53–0.55), indicating distinct assemblages associated with each habitat type. Comparisons with regional datasets from Montana and Washington revealed moderate overlap (Jaccard = 0.22–0.24), suggesting that the Highland Flats farm supports a partly unique bee fauna within the Northern Rockies. Seven non-native bee species and nine species of conservation concern (five Osmia, four Bombus) were detected, with those of conservation concern taxa often visiting native Lupinus flowers. Most bee visits occurred on non-native plants, though native blooms contributed key seasonal resources. These findings demonstrate that organic tree farms with structurally diverse forests and managed floral resources can function as refugia for both common and at-risk bees in temperate forested landscapes. Full article

Global CO₂ concentrations are gradually increasing, and forests, as the main terrestrial carbon pool, are attracting growing attention in mitigating climate change. However, the impacts of forest types, species diversity, structural diversity, and environmental factors on the carbon sequestration mechanisms of subtropical forests remain unclear. This study established 45 forest plots (20 m × 20 m) in Lishui City, aiming to investigate the relationships between forest diversity, environmental factors, and carbon storage of subtropical forests among different forest types. Results showed that coniferous forests had the lowest species diversity (0.86), which exhibited extremely significant differences from broad-leaved forests (1.47, p < 0.01) and coniferous broad-leaved mixed forests (1.58, p < 0.01). The carbon storage of broad-leaved forests was 97.50 t·ha⁻¹, which was higher than that of coniferous broad-leaved mixed forests (77.08 t·ha⁻¹) and coniferous forests (75.57 t·ha⁻¹). The carbon storage of coniferous forests was significantly positively affected by species diversity (p < 0.05). Tree height was the most significant structural diversity factor affecting forest carbon storage (p < 0.05). The results of the structural equation model (SEM) showed that the proportion of broad-leaved trees in forests and structural diversity had a significant positive effect on carbon storage (p < 0.01). Species diversity had a non-linear relationship with carbon storage. The ecological niche complementarity effect and selection effect interacted with changes in species diversity. When the species diversity was lower than 1.12 (Shannon–Wiener index), the ecological niche complementarity effect dominated and promoted carbon sequestration; when it was above this threshold, the selection effect dominated and weakened carbon sequestration. This study recommends prioritizing the planting of broad-leaved tree species during afforestation and paying attention to the current status of forest diversity. Full article

Tree-related microhabitats (TreMs) are small features on living or dead trees that offer habitat, shelter, breeding sites, or food for many organisms, making them useful indicators of forest-dwelling species. Despite increasing research on TreMs in Europe, most published studies have focused on temperate regions, leaving a relative paucity of data from hemiboreal forests. In our research, we aimed to fill the knowledge gap, offering insight into the occurrence patterns and factors influencing TreM diversity in the hemiboreal region. We analyzed data from the National Forest Inventory in Latvia, comprising information on 168,839 trees across 5653 sample plots. The most common TreMs were bark loss (6.1% of trees), bryophytes (2.6%), and perennial polypores (2.6%). TreMs occurred more frequently on deciduous than on coniferous trees, on larger trees (diameter at breast height more than 60 cm), and on dead trees compared to living ones. Forest type and signs of recent cutting also had significant effects on TreM richness at both the tree and plot scales, whereas forest protection status was significant only at the plot scale. TreMs such as buttress-root concavities and ivy or liana cover, which are common in temperate Europe, had a low relative occurrence in our study. The occurrence of specific TreM forms was strongly tree-species dependent: exudates were much more common on live Picea abies (4.0%) than on other species, whereas Populus tremula had a higher occurrence of fruiting bodies of saproxylic fungi and slime moulds (2.0%). The highest occurrence of crown deadwood was observed on Quercus robur. Overall, dead trees play a particularly important role, providing both a higher total number of TreMs and certain TreM types more frequently. Given their high TreM richness, dead and large trees represent important structural components supporting biodiversity in hemiboreal forests. Full article

Poplars (Populus L.) are fast-growing, widely distributed trees with high ecological, economic, and climate-mitigation value, making them central to diverse agroforestry systems worldwide. This study presents a comprehensive bibliometric and content-based review of global poplar-based agroforestry research, using Scopus and Web of Science databases and a PRISMA-guided screening process to identify 496 peer-reviewed publications, covering publications from 1987 to 2024. Results show a steady rise in scientific output, with a notable acceleration after 2013, dominated by agriculture, forestry, and environmental sciences, with strong international contributions and research themes focused on productivity, carbon sequestration, biodiversity, and economic viability. A wide range of Populus species and hybrids is employed globally, supporting functions from crop production and soil enhancement to climate mitigation and ecological restoration. Poplar-based systems offer substantial benefits for soil health, biodiversity, and carbon storage, but also involve trade-offs related to tree–crop interactions, such as competition for light reducing understory crop yields in high-density arrangements, management intensity, and regional conditions. Poplars provide a wide array of provisioning, regulating, and supporting ecosystem services, from supplying food, fodder, timber, and biomass to moderating microclimates, protecting soil and water resources, and restoring habitats, while supporting a broad diversity of agricultural and horticultural crops. However, several critical gaps—including a geographic research imbalance, socio-economic and adoption barriers, limited understanding of tree–crop interactions, and insufficient long-term monitoring—continue to constrain widespread adoption and limit the full realization of the potential of poplar-based agroforestry systems. Full article

Woody debris decomposition is a key process in forest ecosystem material cycles, with invertebrate communities playing a vital role. Distinct physicochemical properties of woody debris types lead to varying effects on these communities. Taking woody debris in Saihanba’s Larix principis-rupprechtii plantations, Betula platyphylla natural secondary forests, and larch–birch mixed forests (northern China) as objects, we collected woody debris-inhabiting invertebrates via hand-sorting. We studied how tree species (larch/birch), forest types (pure/mixed), and decay stages (I–V) collectively regulate invertebrate community assembly. Results showed significant differences in woody debris physicochemical properties across these factors. Phytophagous groups dominated early decay stages (I–III) and decreased significantly (p < 0.05) with reduced wood density. In contrast, saprophagous and predatory groups increased with decay, correlated with higher TN and were more abundant in mixed than pure forests. NMDS indicated significant community differences among tree species/forest types in early decay, converging later. PLS-PM further confirmed functional groups’ response pathways to woody debris characteristics. Thus, preserving woody debris integrity and diversity in plantations is crucial for maintaining invertebrate diversity, promoting nutrient cycling, and enhancing forest ecosystem functions. Full article

Accurate estimation of commercial tree height is essential for volumetric predictions in forest management plans, particularly in Amazonian forests with high species diversity. We assessed two predictive approaches for estimating commercial height, using the sum of actual commercial log lengths as the reference metric. The dataset comprised 1745 harvested trees from Annual Production Unit 8 in the Tapajós National Forest. Three commercial volume groups dominated the structural gradient: 46.1% of the trees Group 1 (<6 m³), 36.7% into Group 2 (6–10 m³), and 17.2% into Group 3 (≥10 m³). The Linear Mixed-Effects Model included diameter at breast height (DBH) as a fixed effect and species as a random effect, whereas the Random Forest model used DBH and species as predictors. The mixed-effects model achieved higher accuracy (r = 0.77; RMSE = 2.95 m), while the Random Forest model performed slightly worse (r = 0.73; RMSE = 3.10 m). Species with greater commercial heights exerted a strong influence on both modelling approaches. Principal Component Analysis revealed structural separation among the three volume groups, driven by DBH, commercial height, number of logs, and log volume. The mixed-effects model provided effective framework for predicting commercial height in heterogeneous tropical forests. Full article

The northern Brazilian Amazon has ecological transition ecosystems with high diversity and endemism of tree species and few botanical collections. We evaluated the phytosociology between Dense Ombrophilous Forest (Ds) and Forested Campinarana (Ld) within Anauá National Forest in Roraima, Brazil. A total of 14,730 trees with a DBH ≥ 10 cm were inventoried across 30 hectares (ha), distributed among 55 botanical families, 183 genera, 386 species, and 123 undetermined trees. Ten hyperdominant tree families accounted for 69% of the sampled trees and 65% of the stored forest carbon (102.9 ± 5.0 Mg ha⁻¹), like Arecaceae (2555 trees), Fabaceae (1738 trees), and Sapotaceae (1311 trees). Ten hyperdominant species accounted for 32% of the sampled individuals and 32% of the stored forest carbon (46.3 ± 3.8 Mg ha⁻¹), like Euterpe precatoria (1151 trees), Pouteria macrophylla (561 trees) and Inga alba (574 trees). Anauá National Forest has great potential for sustainable multiple-use forest management through forest concessions; however, tree mortality due to natural causes and anthropogenic actions (deforestation, illegal selective logging, and forest fires) was considered high (7%) for tropical forests in the Amazon. Full article

The self-heating propensity of the fresh wood of ten tree species (two coniferous, eight deciduous) was studied calorimetrically using oxidation heats, q₃₀, at a temperature of 30 °C. Values of q₃₀ in the range between 0.45 W kg⁻¹ (dry) and 1.1 W kg⁻¹ (dry) were found. The lowest evolution of the oxidation heat proved two coniferous wood types—spruce and pine. On the other hand, the highest value of the q₃₀ heat manifested willow wood, which exceeded (as the only one of the samples) the level of 1 W kg⁻¹ (dry). Water was confirmed to promote the generation of oxidation heat, while completely negligible oxidation heat effects were found in dry wood samples. A rise in the heat evolution with increasing moisture content can be explained not only by a change in the mechanistic pathway of the chemical oxidation of wood in the presence of water, but also by the restoration of the activity of microorganisms in wood, which occurs only at a sufficient level of moisture content. Tree bark appears to be probable carrier of a diverse microbiome. Based on the experiments with debarked wood samples, it can be estimated that the part of the heat produced by microorganisms constitutes a remarkable 35–55% of the global oxidation heat q₃₀, as determined for fresh wood samples. Full article