Search Results (783)

Mechanical confinement plays an important role in regulating tumor growth and invasion; however, the quantitative, time-resolved, three-dimensional evaluation of confined tumor spheroids remains technically challenging. In this study, we developed a custom-built selective plane illumination microscopy (SPIM)-based monitoring platform for long-term volumetric imaging of tumor spheroids under mechanically confined conditions. This system integrates a culture housing unit and a transparent cuvette-based spheroid culture method optimized for SPIM observation. Colorectal adenocarcinoma-derived cell spheroids were embedded in agarose gels with defined concentrations to modulate the stiffness of the surrounding matrix. Bright-field imaging and viability analyses confirmed sustained spheroid growth without necrotic core formation over a 4-day culture period, demonstrating that the SPIM-based system maintained the physiological culture conditions. Three-dimensional imaging using SPIM enabled a quantitative evaluation of spheroid growth and anisotropic invasion. Volumetric expansion was observed under all confinement conditions. Notably, increasing the matrix stiffness enhanced both the volumetric growth rate and anisotropic invasion, indicating stiffness-dependent directional growth under mechanical confinement. The developed SPIM-based platform has the potential to serve as a practical tool for the time-resolved three-dimensional analysis of tumor spheroid growth and may provide a useful approach for investigating the mechanobiological regulation of tumor progression in confined microenvironments. Full article

Neglected tropical diseases (NTDs) such as soil-transmitted helminthiasis, lymphatic filariasis, schistosomiasis, leprosy, rabies, and food-borne trematodiasis are endemic in the Philippines. Despite global and national elimination efforts, these six NTDs remain a persistent burden to the poor, those living in Geographically Isolated and Disadvantaged Areas (GIDAs), and other vulnerable groups. This narrative review synthesized data from Field Health Services Information System (FHSIS) reports of the Philippine Department of Health (DOH) from 2020 to 2024, the available literature from electronic databases, and DOH and WHO reports focusing on the challenges, barriers, and gaps in NTD control and elimination in the country. Core challenges include complex epidemiological landscapes, lapses in disease surveillance, infrastructure, and fragmented health care systems. Gaps include access to diagnostics, insufficient funding and human resource training, and scarcity of local studies focusing on endemic NTDs. With these challenges and gaps, this review highlights the need for a real-time feedback loop system in surveillance strategy, community-based interventions, full integration of NTDs in primary health care, and collaboration between government, NGOs and private entities. Addressing these challenges and gaps is key to shifting from control to elimination. Full article

Background: In recent years, time-lapse incubators (TLs), which can maintain a stable culture environment, have been developed for use in in vitro fertilization (IVF) treatment. Methods: In this retrospective cross-sectional study, data from 1200 women who visited the Creation and Love fertility center between April 2021 and October 2023 were reviewed. Among them, the electronic medical records of 400 women aged 22–45 years who underwent IVF treatment were selected and divided into two groups according to the type of incubator used: 100 in the TL group and 300 in the conventional incubator (CI) group, to evaluate differences in embryo development. Among these participants, pregnancy outcomes were assessed in 150 women who underwent day 3 (cleavage-stage) fresh embryo transfer. Controlled ovarian stimulation was performed using a standard antagonist protocol. The primary outcome was a good embryo rate, and the secondary outcomes were the clinical pregnancy rate and live birth rate. Results: The mean age of the study population was 34.6 ± 5.4 years. The groups had similar good embryo rates (67.7% vs. 65.5%, p = 0.84), clinical pregnancy rates (49.0% vs. 52.0%, p = 0.86), and live birth rates (35.0% vs. 42.0%, p = 0.76). Furthermore, subgroup analyses showed that the live birth rate in women aged ≥ 35 years was higher in the TL group than the CI group (38.0% vs. 23.4%, p = 0.03). Conclusions: Based on the comparative analysis of live birth rates between women cultured using TLs and those using the CI during day 3 fresh-embryo transfer cycles, our study demonstrated a statistically significant increase in live birth rates among women ≥ 35 years in the TL group. Full article

Background/Objectives: Hyaluronic acid (HA) has been proposed as a physiological alternative to polyvinylpyrrolidone (PVP) for sperm immobilization during intracytoplasmic sperm injection (ICSI). This prospective sibling-oocyte study aimed to compare embryological outcomes and morphokinetic parameters between HA and PVP. Methods: A total of 811 sibling-oocytes from 51 ICSI cycles were allocated to the HA group (SpermCatch; n = 377) or the PVP group (10% PVP Solution; n = 434). Fertilization outcomes, cleavage, blastocyst formation, and good-quality embryo development were assessed. Embryo morphokinetic parameters (t2–tB) and an AI-derived embryo score were analyzed in a subset of blastocysts with available time-lapse data. Results: The fertilization rates were comparable between the HA and PVP groups (80.9% vs. 85.3%, p = 0.25), as were the cleavage rates (99.0% vs. 97.0%, p = 0.27). However, the HA group had significantly lower rates of good-quality cleavage-stage embryos (33.4% vs. 47.9%, p < 0.01), blastocyst formation (52.3% vs. 69.9%, p < 0.01), and good-quality blastocysts (49.4% vs. 64.1%, p < 0.01). Morphokinetic timings did not differ significantly, whereas embryos in the HA group showed lower AI scores than those in the PVP group (p = 0.04). Conclusions: In this prospective sibling-oocyte cohort, HA-based sperm immobilization did not improve embryological outcomes compared with conventional PVP-assisted ICSI. Differences observed at the blastocyst stage should be interpreted cautiously and require confirmation in larger, randomized studies with clinical follow-up. Full article

Sikkim, located in the northeastern Himalaya, is highly vulnerable to natural hazards and increasing depletion of surface and subsurface water resources, particularly springs and lakes. In South Sikkim, several lakes exhibit rapid drainage behavior, among which Nagi Lake shows near-complete water loss shortly after rainfall, indicating the presence of subsurface leakage pathways. This study investigates shallow subsurface moisture dynamics and identifies potential seepage-prone zones beneath the Nagi Lake basin using geoelectrical methods. Electrical resistivity profiling was conducted along seven survey lines during the non-rainy season (October–November 2025) to minimize the influence of transient rainfall-induced moisture variations. Profiling was carried out using the Wenner method, achieving investigation depths of approximately 6.5–9 m. Additionally, Vertical Electrical Sounding (VES) using the Schlumberger configuration was performed at selected locations to examine deeper subsurface conditions. Resistivity results indicate that profiles L1, L2, L3, L4, and L7 contain relatively higher moisture restricted to the upper ~5 m, whereas profiles L5 and L6 exhibit persistently low resistivity values from the surface to depths of ~9 m, suggesting sustained subsurface moisture accumulation. The downward extension of low-resistivity zones along L5 and L6 indicates possible preferential seepage pathways or localized subsurface water storage. VES results further reveal a higher density of subsurface anomalies below ~14 m in these areas. These low-resistivity anomalies are interpreted as potential subsurface flow pathways. Although confirmation of active seepage requires additional hydrological or time-lapse investigations, the findings provide important baseline geophysical insights for lake rejuvenation. Full article

Overwintering peat fires are increasingly reported in the boreal regions, where they persist underground through winter and reignite in spring, intensifying greenhouse gas emissions and landscape degradation. This study investigates the conditions that enable peat fires to survive freezing and snow cover, and presents practical methods for their winter detection and suppression. We combined satellite data, UAV-based thermal imaging, time-lapse photography, and ground measurements of temperature, groundwater depth, and peat moisture to identify active overwintering hotspots. Our results show that these fires persist primarily where groundwater levels remain below 60 cm, particularly under tree roots, compacted soil, or elevated terrain that limits moisture recharge. UAV thermal imaging proved the most reliable detection tool, identifying 98% of hotspots. We developed and successfully applied a winter extinguishing method that involves mechanical disruption and dispersion of smoldering peat over frozen ground, allowing rapid cooling without re-ignition. These findings clarify the mechanisms sustaining overwintering fires and provide an effective approach for their mitigation, contributing to reduced emissions and improved management of boreal peatlands vulnerable to climate change. Full article

Background: Oral squamous cell carcinoma (OSCC) is an aggressive cancer closely associated with smoking and alcohol consumption, with a higher incidence in men. Despite changes in treatment strategies, poor survival persists in most patients, highlighting the need for novel and improved therapeutic options. Naphthoquinone analogs are being investigated because of their active redox structure and broad pharmacological profile; they demonstrate cytotoxic antitumor activity, making them potential candidates for new drug agents. Objective: This study investigated new benzyl-naphthoquinone compounds as potential anticancer agents for various genotypes of oral squamous cell carcinoma (OSCC) and other cancer cells. Methods: This study reports the synthesis and evaluation of a series of eight benzyl-naphthoquinone compounds against oral squamous cell carcinoma. Results: Four compounds 1–4 showed the best cytotoxic profiles, with a selectivity index ≥ 3 for all OSCC cell lines tested. Compound 1 was the most selective compound in all OSCC models, showing a higher selectivity index than both carboplatin and shikonin. Furthermore, compound 1 induced DNA fragmentation, cell-cycle arrest, and caspase-3/7 activation, changes consistent with apoptosis, and time-lapse imaging corroborated the apoptotic phenotype. Hemolysis assays showed minimal toxicity in human erythrocytes, and acute in vivo evaluation in mice revealed no evident adverse effects under the conditions tested, indicating low acute toxicity, although more detailed histopathological and biochemical studies will be required to fully establish the safety profile. Molecular modeling suggested that compound 1 may interact with topoisomerase II, RSK2, and PKM2, which could contribute to the activation of apoptotic pathways, although these interactions remain predictive and require biochemical validation. Finally, in silico analysis of physicochemical and ADMET parameters indicated properties compatible with oral absorption and systemic exposure, together with predicted low toxicity; however, these results are model-based and should be confirmed experimentally. Conclusions: Based on these findings, compound 1 emerges as a promising lead candidate for the development of a novel chemotherapeutic agent against OSCC, with potential therapeutic efficacy against other cancer types. Full article

Snow avalanches pose a serious hazard in snow-covered, mountainous areas. In order to protect inhabited areas and infrastructure such as roads and railway lines, avalanche protection measures need to be taken. In addition to permanent, technical protection measures, temporary, organizational measures, which are based on risk assessments by local avalanche warning commissions, are utilized. These avalanche risk assessments rely on regional avalanche bulletins, weather forecasts, local expertise, and information on current snowpack conditions. Our research seeks to enhance knowledge of current snowpack and avalanche conditions by providing in situ monitoring of potential avalanche slopes. Therefore, we developed a novel sensor box array, peakr, consisting of multiple sensor units deployed by hand or by drone at key avalanche slope locations throughout the winter season. The sensors continuously measure temperature, humidity, position, and snowpack movement. Data are transmitted via LoRaWAN and GSM, stored locally, and accessed through a web platform. Automated analysis using a decision tree and event-detection algorithm triggers immediate alerts to responsible personnel via SMS and email. This paper presents an overview of the peakr sensor array and web platform, focusing on data analysis and avalanche events from the Arlberg ski resort in winter 2023/2024, supported by webcam time-lapse validation. Full article

The complex architecture and stacking patterns of deepwater turbidite channel sandbodies introduce significant uncertainty in injector–producer connectivity. This uncertainty affects both the mechanisms and the quantitative evaluation of the waterflood sweep. In this study, a representative reservoir in the Niger Delta Basin is selected as a case study. Injector–producer well groups are first classified into three connectivity patterns—coeval, cross-stage, and hybrid based on geological and seismic constraints. Time-lapse seismic data are then interpreted to delineate sweep morphology and to infer the controlling mechanisms associated with each pattern. Coeval connectivity exhibits a relatively uniform and continuous front advance with minimal barriers. Cross-stage connectivity shows fragmented swept regions with pronounced bypassing, and localized preferential breakthrough caused by discontinuous sandbodies and pervasive barriers. Hybrid connectivity is characterized by intermediate behavior, combining features of both patterns. To translate these mechanistic differences into quantitative metrics for development evaluation, an oil–water relative permeability ratio correlation for low viscosity oil is established that remains valid across the full water cut range, thereby overcoming the limitations of conventional semi-log linear correlations at both low and ultra-high water cut stages. Based on this framework, a production data-driven predictive model for waterflood sweep efficiency is derived using production data and steady state flow theory. The model is validated across well groups representing different connectivity patterns. Field application yields a consistent ranking of sweep efficiency: coeval > hybrid > cross-stage, with group average values of 0.86, 0.80, and 0.70, respectively. These results agree with the mechanistic interpretation derived from time-lapse seismic analysis. The proposed methodology provides a practical quantitative framework for evaluating injector–producer connectivity and comparing development strategies in deepwater turbidite channel reservoirs. Full article

This systematic review evaluates recent scientific and technological advances in water quality monitoring and pollution alarms for ports, based on records retrieved from seven databases following the PRISMA protocol. A total of 414 documents were screened, resulting in 141 articles (TRL 3) and 56 patents (TRL 4–5). Bibliometric, patentometric, and thematic analyses were conducted using Bibliometrix and ORBIT^®. Results show sustained growth in both academic and technological outputs, with a patent Compound Annual Growth Rate (CAGR) of 32%, compared with 13% for scientific publications, indicating accelerated translation from research to innovation. The conversion rate from scientific research to patenting increased from 14% (2010–2015) to 47% (2020–2023). Analysis of patent legal status reveals that 52% of patent families remain valid (48% granted; 4% pending), while 33% are lapsed, 13% revoked, and 2% expired, reflecting the dynamic and emerging character of the field. Technological ownership is highly concentrated, with China accounting for nearly all active patents, whereas scientific production is more geographically distributed. Thematic analysis identifies four main scientific clusters: environmental monitoring, chemical pollutants, seashore hazards, and eutrophication. The main technological domains of the patents are analysis of biological materials, control, and environmental technologies. Emerging areas of focus at TRL 3 and TRL 4–5 include microplastics, climate-change impacts, aquaculture risks, real-time sensing, IoT-enabled platforms, machine-learning analytics, autonomous monitoring systems, and bioindicator-based early-warning tools. This review provides a quantitative roadmap to support sustainable port operations, coastal ecosystem protection, and progress toward multiple synergistic United Nations Sustainable Development Goals (SDGs). Full article

Background: Does maternal body mass index (BMI) influence embryo morphokinetics in fresh embryo transfer cycles, and how does this relate to clinical outcomes and obstetric complications? Methods: A retrospective cohort study was conducted on 2238 fresh embryo transfer (ET) cycles, categorized into four BMI groups: underweight, normal weight, overweight, and obese. Baseline characteristics, stimulation parameters, hormonal profiles, morphokinetic data, and pregnancy and delivery outcomes were analyzed. Results: Higher BMI was associated with more anovulatory infertility and greater endometrial thickness. Peak estradiol and estradiol-to-oocyte ratios declined progressively with increasing BMI, despite preserved oocyte yield and embryo quality scores. Interestingly, the underweight group exhibited a significantly distinct biphasic morphokinetics developmental pattern compared with the overweight and obese groups. Pregnancy rates, including clinical and live birth, did not differ significantly across BMI groups. However, obese women had markedly higher cesarean section rates (51.9% vs. ~25–28% in other groups) and a non-significant trend toward more gestational diabetes. Other perinatal outcomes, such as preeclampsia and preterm birth, were not significantly different. Conclusions: In fresh IVF cycles, a higher BMI does not impair pregnancy achievement but is linked to altered hormonal response and increased obstetric risk, particularly cesarean delivery. These findings highlight the importance of preconception counseling and targeted obstetric management for women with elevated BMI undergoing fresh ET. Full article

Background/Objectives: Adolescence is a critical period for executive function (EF) maturation. While sleep and physical activity (PA) are key lifestyle factors, their longitudinal impact on EF in ecologically valid settings is insufficiently characterised. This study examined the associations between objectively measured sleep duration, daily steps, and EF performance across one academic year (~9 months). Methods: A longitudinal study was conducted with 168 Spanish adolescents (13–16 years). Sleep duration and daily steps were monitored using Fitbit Charge 6 wearables for 7-day periods at baseline (M1; September 2024) and follow-up (M2; June 2025). EFs were assessed using three validated tasks: Stroop (inhibitory control), Psychomotor Vigilance Task-Brief (PVT-B; sustained attention), and Paced Auditory Serial Addition Test (PASAT; working memory). Linear Mixed Models (LMM) were employed to analyse the effects of the fixed factors (i.e., Group and Time), and their interactions. Results: PA, but not sleep duration, significantly predicted executive performance. The High_PA group demonstrated faster reaction times in inhibitory control (p = 0.007) and significantly fewer attentional lapses in sustained attention (p = 0.014). In contrast, sleep duration showed no significant main effects on EF domains (p > 0.05). Regression analyses confirmed that higher daily steps predicted faster reaction times in inhibitory control in the total sample (r = −0.173, p = 0.002), although an unexpected positive association was observed in the Low_PA group for inhibitory control, warranting cautious interpretation. Conclusions: These findings suggest that habitual PA is associated with better EF performance in adolescents, whereas sleep duration alone (without considering timing or variability) showed no significant associations with cognitive outcomes. Sensitivity analyses using clinically informed thresholds and continuous standardised predictors confirmed the robustness of these findings. Full article

Background/Objectives: Dynamic remodeling of the zona pellucida (ZP) is a fundamental biochemical and structural process during human preimplantation development; however, its quantitative characterization and clinical relevance remain incompletely defined. The objective of this study was to evaluate dynamic ZP thinning as a functional marker of embryo developmental competence and to examine its relationship with follicular fluid (FF) biomarkers and clinical pregnancy. Methods: This prospective observational study included 47 IVF cycles performed at a single center, yielding 64 transferred blastocysts with complete time-lapse data. ZP thickness was measured from fertilization to 120 h post-fertilization using time-lapse imaging. Two quantitative parameters were derived: the relative thinning ratio (Δrel) and the linear thinning rate (slope). FF concentrations of growth differentiation factor 9 (GDF-9), hyaluronic acid (HA), and syndecan-4 (Syn4) were quantified by ELISA. Embryo-level associations with spontaneous blastocyst hatching were assessed using logistic regression and multivariate analyses, while patient-level models evaluated predictors of clinical pregnancy. Results: Embryos that underwent spontaneous hatching exhibited significantly greater Δrel than non-hatching embryos (p < 0.001). Δrel remained the strongest predictor of hatching in multivariable models (AUC = 0.91). Among FF biomarkers, only GDF-9 showed a positive association with spontaneous hatching. At the patient level, higher Δrel values of transferred embryos were associated with clinical pregnancy (OR 3.65, p = 0.009), whereas FF biomarkers and assisted hatching showed no significant association. Conclusions: Dynamic ZP thinning quantified by Δrel represents a promising indicator of embryo developmental competence. The concordance between embryo-level hatching behavior and patient-level clinical pregnancy suggests potential clinical relevance of ZP dynamics as an integrative embryological marker, warranting validation in larger cohorts. Full article

For effectively assessing blood, red blood cell (RBC) aggregation and blood viscosity have been measured in microfluidic environments. However, the previous methods still face several challenges (dead-volume loss, RBC sedimentation, hematocrit-sensitive blood velocity, and precise flow rate control). In this study, a novel method is suggested to resolve several issues. Air cavity (V_air = 250 μL) is secured above the blood column (at least 100 μL) loaded into a driving syringe. To probe RBC aggregation and blood viscosity, a microfluidic chip consists of a main channel ($\dot{\gamma }$ > 1000 s⁻¹) and an aggregation channel ($\dot{\gamma }$ < 50 s⁻¹). Blood is supplied into a microfluidic chip with two-step blood delivery (i.e., air compression for RBC aggregation, and syringe pump for blood viscosity). RBC aggregation index and blood viscosity are obtained from time-lapse image intensity and blood flow rate in both channels. As performance demonstrations, first, the measurement accuracy of fluid viscosity is validated with glycerin solution. Then, the present method is adopted to probe the difference in hematocrit and dextran concentration. At last, the proposed method is employed to detect heat-shocked RBCs (45~50 °C for 40 min). In conclusion, the proposed method has the ability to accurately measure substantial changes in RBCs or blood medium. Full article

Background: In Pakistan, the number of Human immunodeficiency virus (HIV) cases is increasing significantly, attributed to risk factors such as injection drug use, sexual transmission, etc. However, transmission through hemodialysis units is not well documented. In 2024, an outbreak of HIV cases in Multan, Pakistan, drew alarm from local health authorities due to reports linking it to a large public hospital in South Punjab. Here, we report the molecular epidemiological investigation of the outbreak. Methods: Twenty-five hemodialysis patients identified during the outbreak were enrolled. Blood samples were subjected to DNA extraction and polymerase chain reaction (PCR) amplification. Phylogenetic analysis was conducted using the maximum-likelihood approach in IQ-TREE. For dating phylogenetics, a maximum clade credibility tree (MCC) was constructed using the BEAST tool. The MCC tree was constructed using the Bayesian Skyline model with an uncorrelated lognormal relaxed clock. The VESPA program was used to identify amino acid signatures unique to outbreak sequences compared with Pakistani reference sequences. Results: A total of 25 patients (identified as part of the HIV outbreak) were enrolled. 96% (24 out of 25) also tested positive for Hepatitis C, while none tested positive for Hepatitis B. The age range of patients in the study was 23 to 72 years (median age: 44.88 years). In terms of gender distribution, 13 out of 25 were male. All the sequences were identified as HIV subtype CRF02_AG. Phylogenetic analysis revealed that Multan sequences formed a well-supported monophyletic cluster, indicating shared recent origin. Signature pattern analysis identified a unique molecular fingerprint at 26 nucleotide positions, whereas molecular dating placed the emergence of the cluster between 2023 and 2024, consistent with the outbreak timing. Conclusions: Findings provide biologically plausible evidence of a point-source HIV outbreak linked to lapses in infection prevention and control practices at the hemodialysis unit. Full article