Search Results (8,233)

Despite increasing interest in probiotics as antibiotic alternatives in swine production, few studies have directly compared the functional efficacy of different commercial probiotic formulations under controlled conditions. We conducted an in vitro study using porcine intestinal epithelial (IPEC-J2) and macrophage-like (3D4/21) cell models to compare the efficacy of three commercial probiotic consortia (C1: three strains of Bacillus velezensis; C2: B. licheniformis + B. subtilis; C3: Clostridium butyricum). Treatments were evaluated for their ability to inhibit pathogenic Escherichia coli, Clostridium perfringens, and Salmonella spp., enhance epithelial barrier integrity, and modulate immune responses. Experimental endpoints included pathogen inhibition assays, adhesion to IPEC-J2 cells, transepithelial electrical resistance (TEER), tight junction protein expression, and cytokine profiling via RT-qPCR and proteomics. Data were analyzed using the Kruskal–Wallis test with false discovery rate (FDR) control at 5%. C1 cell-free supernatant (CFS) strongly inhibited pathogen growth (84.8 ± 5.3% inhibition of ETEC F4⁺F18⁻ vs. medium control; p < 0.05), whereas C2 had no effect, and C3 inhibited only one isolate. The coculture of IPEC-J2 cells with C1 CFS increased the expression of TJ proteins ZO-1, MUC13, and MUC20 (+12.9–46.6% vs. control; p < 0.001) and anti-inflammatory TGF-β; reduced pro-inflammatory IL-6 in LPS-stimulated 3D4/21 cells. In comparison, C2 and C3 showed minimal impact on epithelial barrier integrity and immune modulation, as indicated by negligible changes in TEER values, tight junction protein expression (ZO-1, MUC13, MUC20), and cytokine profiles relative to the control. In conclusion, C1 demonstrated greater in vitro efficacy than C2 (B. licheniformis + B. subtilis) and C3 (Clostridium butyricum), including pathogen inhibition assays, epithelial adhesion, TEER measurements, and cytokine modulation, suggesting its potential as a leading candidate for functional probiotic applications. Full article

The creation of a specific culture medium for colorectal organoids in 2011 heralded a new era in human primary cultures by enabling the indefinite expansion of normal and pathological epithelial organoids. The original formula has been used ever since, with only minor, lab-specific modifications. The goal of culturing organoids from different tissues has relied on saving and propagating the pluripotent stem cell. The “magic bullet” and all its subsequent derivatives have pursued this goal. Consequently, agonist and antagonist signals are chronically activated in the organoid medium, forcing organoid cells (as well as any other co-cultured cellular model) into constrained signaling pathways. This extremely artificial condition is often overlooked in experimental approaches and may bias the results. Furthermore, some molecules in the organoid medium have unpredictable off-target effects that significantly impact the behavior and maturation of certain cell populations. Nicotinamide, gastrin and PGE2 inhibit immune responses. SB202190, A83-01 and vanadate (from advanced DMEM-F12) modify intracellular signaling. N-AcetylCysteine and Primocin modify the redox response and mitochondrial metabolism, respectively. Thus, the unintentional addition of these molecules to the organoid medium introduces biases under specific experimental settings. While the original organoid medium formula is the gold standard for propagating organoids in vitro, more focused, reliable conditions are necessary for specific organoid-based tests. Full article

This article presents a hybrid optimization model designed to determine the optimal location and operation of capacitor banks in medium-voltage distribution networks, aiming to reduce energy losses and enhance the system’s economic efficiency. The use of reactive power compensation through fixed-step capacitor banks is highlighted as an effective and cost-efficient solution; however, their optimal placement and sizing pose a mixed-integer nonlinear programming optimization challenge of a combinatorial nature. To address this issue, a multi-objective optimization methodology based on the Sine Cosine Algorithm (SCA) is proposed to identify the ideal location and capacity of capacitor banks within distribution networks. This model simultaneously focuses on minimizing technical losses while reducing both investment and operational costs, thereby producing a Pareto front that facilitates the analysis of trade-offs between technical performance and economic viability. The methodology is validated through comprehensive testing on the 33- and 69-bus reference systems. The results demonstrate that the proposed SCA-based approach is computationally efficient, easy to implement, and capable of effectively exploring the search space to identify high-quality Pareto-optimal solutions. These characteristics render the approach a valuable tool for the planning and operation of efficient and resilient distribution networks. Full article

There are many studies showing fitness and/or sporting skill is associated with better cognitive performance. One mechanism proposed to explain these effects is increased neural plasticity, meaning better cognitive performance could be the result of a more trainable brain. This theory was tested by looking at the initial performance and the performance following training on a visual search task for individuals engaged in sports and for control individuals. Analysis of variance for speed of task performance with factors of group, gender and sport for both speed of responses on the task and improvement in response times with practice showed no significant effects (analysis of variance F < 1.9, p > 0.175 for all group effects). The only significant difference was a reduced difference between specific and non-specific learning on the task, likely indicative of reduced non-specific learning in some of the sports groups (runners/controls, ANOVA F(1, 43) = 5.484, p = 0.024, η_p² = 0.113 a medium effect size; and male baseball, runners and controls F(2, 33) = 3.427, p = 0.044, η_p² = 0.172 a large effect size), possibly due to previous improvement because of fitness or sporting skill. These findings suggest a need for specificity in terms of selecting sport training when trying to produce cognitive benefits and a need for better assessment of sport-specific and sport/fitness-general effects on cognitive performance. Full article

Background/Objectives: Stool-based GeneXpert testing has become a useful approach for diagnosing pediatric pulmonary tuberculosis (PTB). This study compared two stool-processing methods, centrifugation-based processing (CBP) and simple one-step (SOS), for detecting PTB in children using Xpert MTB/RIF Ultra (Ultra). Methods: Children with presumptive PTB were screened cross-sectionally, and stool samples were collected and tested with Ultra using the CBP method from March 2022 to December 2024 across seven divisions of Bangladesh. A subset of stool samples (n = 281) that tested positive (n = 191) and negative (n = 90) by the CBP method were re-tested again with the same sample by Ultra using the SOS method. The results of the Ultra with SOS-processed stool were compared with the CBP method to evaluate overall agreement and detection efficiency across different bacterial burdens. Results: The SOS method detected 97 of 191 CBP-positive samples, resulting in a positive percentage agreement of 50.8% (95% CI: 43.5–58.1). All 90 Ultra-negative stool were also negative by the SOS method, yielding a negative percentage agreement of 100% (95% CI: 96.0–100.0). Overall agreement between the methods was 66.6% (Kappa: 0.398). The SOS method detected 100% of high- (4/4) and medium- (7/7), 97.3% (36/37) of low-, and 83.3% (35/42) of very-low-bacterial-burden samples, but only 14.9% (15/101) of the trace-detected samples that were identified by the CBP method. Conclusions: Stool testing with Ultra using the SOS processing method missed a significant number of the most prevalent form of child TB—the ‘trace-detected’ category identified by the CBP method. For increased detection of childhood TB nationwide, the national program should prioritize the use of Ultra on stool samples processed by the CBP method. Full article

This study examines outcomes associated with a short-term intensive pedagogical experience aimed at developing social entrepreneurship competencies among university students at an Ashoka U–affiliated institution in Mexico. The program, Semana Tec de Agencia de Cambio, is a five-day experiential learning experience grounded in the SEL4C (Social Entrepreneurship Learning for Complexity) framework and designed to promote changemaking through interdisciplinary collaboration, reflection, and action. Using a quantitative quasi-experimental pre–post design (n = 210), data were collected through the validated Social Entrepreneur Profile (SEP), which assesses four dimensions: self-control, leadership, social awareness and social value, and social innovation and financial sustainability. Paired-samples t-tests indicated statistically significant increases (p < 0.001) across all dimensions, with small to medium effect sizes (Cohen’s d = 0.40–0.63). Multiple regression analysis showed that changes in social awareness and social value (β = 0.33, p < 0.001), leadership (β = 0.27, p = 0.004), and innovation and sustainability (β = 0.24, p = 0.006) were most strongly associated with overall changes in self-perceived competencies, explaining 58% of the variance (R² = 0.58). Overall, the findings suggest that short-term intensive educational experiences grounded in active and interdisciplinary pedagogical approaches may contribute to measurable changes in students’ self-perceived social entrepreneurship competencies. Rather than evidencing consolidated transformation, the results are best interpreted as early indicators of competency activation within changemaker-oriented learning environments. The study contributes empirical insight into the use of intensive formats in social entrepreneurship education and situates the SEL4C framework as a coherent pedagogical reference within the Ashoka U context, without implying causal validation. Full article

In the marine environment, numerous factors endanger the preservation of underwater rock surfaces as well as submerged archeological artifacts, including physical, chemical, and biological processes. Limestone and marble are common materials used in artifacts due to their availability and long-term durability. However, such surfaces provide a suitable substrate for the settlement of micro- and macro-organisms, causing so-called biofouling, which significantly contributes to stone deterioration. Previous studies have demonstrated the applicability of antifouling coatings containing ionic liquids (ILs) on marble surfaces and assessed their durability for up to 15 days under submerged environments. To further corroborate these results, additional physical studies (colorimetric, contact angles, capillarity water absorption measurements, and UV aging) were carried out on treated limestone. Washout tests were also performed on both lithotypes to verify the coatings’ stability under medium-term underwater exposures. The results of these investigations are reported here. Our data confirm that the application of IL-based coatings had no effect on the intrinsic properties of the limestone surfaces, as previously reported for marble, including resistance to daily UV irradiation. In addition, laboratory tests demonstrated good coating durability against seawater erosive action for up to 6 months. Full article

This paper proposes a wavefront-based method for detecting and locating single-line-to-ground faults in distribution lines using only the transient waveform recorded at one line terminal. The measured current is transformed into a time–frequency representation by the S-transform, and a low-rank structure is extracted by truncated singular value decomposition to suppress broadband noise. On this basis, a hysteresis-type energy envelope is constructed to determine the onset of the fault surge front. To distinguish the genuine fault echo—the main reflection associated with the fault location—from branch echoes and terminal ringing, three complementary criteria are combined: a generalized likelihood ratio test on the time–frequency energy, a dual-pulse interval matching based on the expected round-trip time between the terminal and the fault, and a multi-band consistency check over low-, medium-, and high-frequency components. Numerical experiments under different fault locations and signal-to-noise ratios show that the proposed method improves the average echo recognition rate by about 3.5% compared with conventional single-criterion detectors, while maintaining accurate wavefront-onset estimation with MHz-class sampling (1–5 MHz) that is readily available in practical on-line travelling-wave recorders, rather than relying on ultra-high sampling (e.g., tens of MHz and above). The method therefore offers a physically interpretable and practically feasible tool for fault-echo detection in overhead distribution feeders. Full article

Wildfires are increasingly recognized as a climatological hazard, able to threaten industrial and critical infrastructure safety and operations and lead to Natech disasters. Future projections of exacerbated fire regimes increase the likelihood of Natech disasters, therefore increasing expected direct damage costs, clean-up costs, and long-term economic losses due to business interruption and environmental remediation. While large industrial complexes, such as oil, gas, and chemical facilities have sufficient resources for the implementation of effective prevention and mitigation plans, small-to-medium-sized industrial hubs are particularly vulnerable due to their scattered distribution and limited resources for investing in comprehensive fire prevention systems. This study targets the vulnerability of these communities by proposing the deployment of Wireless Sensor Networks (WSNs) as cost-effective Early Wildfire Detection Systems (EWDSs) to safeguard wildland and industrial domains. The proposed approach leverages wildland–industrial interface (WII) geospatial data, simulated wildfire dynamics data, and mathematical optimization to maximize detection efficiency at minimal cost. The WII delimits the boundary where the presence of wildland fires impacts industrial activity, thus representing a proxy for potential Natech disasters. The methodology is tested in Cocentaina, Spain, a municipality characterized by a highly flammable Mediterranean landscape and medium-scale industrial parks. Results reveal the complex trade-offs between detection characteristics and the degree of protection in the combined wildland and WII areas, enabling stakeholders to make informed decisions. This methodology is easily replicable for any municipality and industrial installation, or for generic wildland–human interface (WHI) scenarios, provided there is access to wildfire dynamics data and geospatial boundaries delimiting the areas to protect. Full article

This study investigated the removal of phosphates from water using an adsorbent composed of Sacha Inchi cuticle and eggshell. For this, a filtration system was constructed using 10 cm diameter PVC pipes and employing 155.56 g of the adsorbent, which corresponded to a filtering medium volume of 1.3 × 10⁻³ m³. With these design parameters, a working flow rate of 0.020 m³/h and a filtration velocity of 2.52 m³/(m²·h) were established. To test the system, 8 L of a solution with a concentration of 133 mg/L of PO₄⁻³ was prepared. As a result, a PO₄⁻³ removal percentage of 35.58 ± 1.57% was obtained. Full article

This research aimed to assess the effect of chitosan nanoparticles (ChNPs) during in vitro shoot proliferation of vanilla using temporary immersion bioreactors (TIB). TIB culture is a biotechnological process that uses semiautomated containers for the production of explants exposed in liquid culture medium. Concentrations of control, 25, 50, 100, 200, and 400 mg/L ChNPs were evaluated in Murashige and Skoog culture medium. Morphological characterization of ChNPs was performed using scanning electron microscopy. At 60 days of culture, survival (%), development variables, photosynthetic pigment content, lipid peroxidation expressed in malondialdehyde, total phenolic content (TPC), hydrogen peroxide (H₂O₂) content, and total antioxidant capacity (TAC) expressed in trolox equivalents were evaluated. The data were analyzed with analysis of variance, with a Tukey test (p ≤ 0.05) using SPSS statistics software, version 29. The results revealed that the greatest survival (%) was obtained at concentrations of control, 25, and 50 mg/L ChNPs, while the lowest survival (%) was observed at concentrations of 400 mg/L ChNPs. Growth stimulation was found, as well as an increase in chlorophyll and β-carotene at concentrations of 25 and 50 mg/L ChNPs. The level of H₂O₂ increased at 25 and 50 mg/L ChNPs. Lipid peroxidation showed no differences among treatments. TPC increased at 100 and 200 mg/L ChNPs, while TAC increased at 200 and 400 mg/L ChNPs. In conclusion, the administration of ChNPs at low concentrations can stimulate growth, while at high concentrations they can inhibit it, a response known as hormesis or hormetic effect. Full article

Bee-derived products such as apitoxin, royal jelly, propolis, bee pollen, and honey are increasingly being used as part of cosmetic products because all of them contain a large number of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial, and regenerative properties, which enable them to be used for therapeutic purposes. The aim of this investigation was to assess the performance of an automated growth-based system in order to make a quantitative examination of the total aerobic viable counts in bee-derived personal care products using NF-TVC vials that contained a nutrient-based medium with dextrose as the carbon source. According to USP general chapter <1223>, pivotal validation criteria such as linearity, equivalence of results, operative range, precision, accuracy, ruggedness, limit of quantification, and limit of detection have demonstrated that the automated system can be used for a reliable total aerobic viable count. Moreover, the actual research demonstrated that polysorbates efficiently block the antimicrobiological potential of bioactive compounds, such as phenols, flavonoids, enzymes, peptides, and fatty acids, which naturally occur in apitoxin, royal jelly, propolis, bee pollen, and honey, allowing for efficient microorganism recovery from the bee-made products tested. Therefore, this AGBS could be applied efficiently within the cosmetic industry to assess the total aerobic viable count in bee-derived products such as capillary treatments, toothpaste, and anti-aging cream, affording several benefits associated with faster product release into the market. Full article

This study evaluates the hydrogen embrittlement (HE) resistance of nickel-based electroplated coatings applied on cold-finished mild steel, with emphasis on their performance as diffusion barriers to impede hydrogen ingress. Nickel coatings were deposited using Watts plating bath under controlled electroplating parameters. Electrochemical hydrogen charging was performed in an alkaline medium at progressively increasing charging current densities to simulate varying levels of hydrogen exposure. Tensile testing was conducted immediately after charging to assess the mechanical response of both uncoated and nickel-coated specimens, focusing on key properties such as elongation, yield strength, ultimate tensile strength, and toughness. The results revealed a gradual degradation in ductility and toughness for the uncoated steel samples with increasing hydrogen content. In contrast, the nickel-coated specimens maintained mechanical stability up to a critical hydrogen threshold, beyond which a pronounced reduction in tensile response was observed. Fractographic analysis supported these trends, revealing a transition from ductile to brittle fracture characteristics with increasing concentrations of hydrogen. These findings highlight the protective capabilities and limitations of nickel-based coatings in mitigating hydrogen-induced degradation, offering insights into their application in industries where hydrogen embrittlement of structural materials is a major concern. Full article

Medium-entropy alloys (MEAs) represent a promising class of materials for solid-state hydrogen storage due to their high hydrogen affinity, structural stability, and tunable properties. In this work, a compositional series of (TiZrNbTa)_{100−x}Al_x (x = 0–10 at. %) MEAs were prepared and systematically investigated to clarify the influence of aluminum addition on microstructure, mechanical response, and hydrogen sorption behavior. The alloys were synthesized by arc melting, homogenized by annealing, and characterized using microscopy, X-ray diffraction, density measurements, microhardness testing, nanoindentation, and hydrogen absorption/desorption experiments. Hydrogen sorption was evaluated by isobaric absorption measurements at 2 MPa H₂ over two consecutive cycles, complemented by thermogravimetric desorption analysis of hydrogenated samples. The results show that aluminum addition significantly affects activation behavior, hydrogen uptake, and residual hydrogen retention, while simultaneously increasing hardness and elastic modulus in a non-linear manner. The alloy containing 5 at. % Al exhibits the most balanced performance, combining reduced activation temperature in the second absorption cycle, relatively high hydrogen capacity, and moderate mechanical stiffness. These findings demonstrate that controlled aluminum alloying is an effective strategy for tailoring hydrogen–metal interactions and optimizing the performance of TiZrNbTa-based MEAs for solid-state hydrogen storage applications. Full article

Roadways driven along the floor of thick coal seams, while retaining the top coal, form “thick coal seam floor roadways.” These large-section roadways feature a composite coal-rock roof and weak coal ribs, leading to low overall strength and poor stability of the surrounding rock. Significant deformation and “necking” often occur, accompanied by roof falls and rib spalling, which are exacerbated under high stress or adverse geology, threatening mine safety and production. In this study, the 2201 haulage gateway in Yingpanhao Coal Mine is investigated to address surrounding rock control in such deep roadways. Using field investigation, theoretical analysis, numerical simulation, and similar simulation tests, the failure mechanisms of ribs and roofs are analyzed. Rib failure is characterized by tensile fracture in the shallow zone, splitting failure in the medium-depth zone, and incomplete conjugate shear in the deep zone. Corresponding mechanical models are established, and a method for calculating total rib failure depth—combining tensile/splitting and shear failure depths—is proposed, along with a bolt length design formula. Based on this, a synergistic roof-and-rib support technology is developed. The failure mechanism and optimal support scheme are validated through simulation tests and successfully applied in the field, demonstrating satisfactory performance. The findings provide a valuable reference for support design in similar mining roadways. Full article