The Role of Probiotics in Animal Health

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 2114

Special Issue Editors


E-Mail Website
Guest Editor
Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolás Cabrera, 9. Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, Spain
Interests: foodborne pathogens; antimicrobial agents; food by-products; food bioactive compounds; food control
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor Assistant
Department of Food Safety and Quality, Lithuanian University of Health Sciences, Veterinary Academy, Tilzes St. 18, LT-47181 Kaunas, Lithuania
Interests: foodborne pathogens; food safety and quality

Special Issue Information

Dear Colleagues,

Probiotics are live microorganisms considered a safe and effective measure to promote animal health when administered in adequate amounts. Probiotics are used as a feed additive to improve intestinal microbial balance. The benefits include the reduction in infection risks, improvement of growth performance, feed utilization, and stress response in animals. It can also be used as an alternative to prophylactic use of antibiotics; therefore, it is important when facing the challenge of antimicrobial resistance.

Probiotics include various bacterial species, bacteriophages, microalgae, and yeasts, and can be used in terrestrial animals and aquaculture. Commercially available feed additives may contain mono or multi-strains and work through bactericidal and bacteriostatic mechanisms. Bactericidal action is responsible for the direct inactivation of pathogens in the gut with the help of bacteriocins, hydrogen peroxide, and organic acids, while competition for nutrients, adhesion sites, and changes in environmental conditions may lead to a bacteriostatic effect.

The use of probiotics may also face several challenges. The selection of suitable microorganisms or their combinations, the amount of viable cells, their survivability, and the application type for various animal species, taking into account age and physiological state, must be considered.

This Special Issue welcomes original articles on studies of microorganisms known as probiotics and their usage in animal health promotion.

Potential topics include, but are not limited to, the following:

Potential new probiotic species and their safety concerns; Molecular methods as a way to characterize the mechanism of action and choose suitable formulations of probiotic strains; Probiotics as a disease control measure in animal life; Probiotics as a means to control antimicrobial resistance; Efficiency related to application method and the fate of probiotics in the gastrointestinal tract.

Dr. Jose Manuel Silvan
Guest Editor

Dr. Gintare Zakariene
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • probiotic strains
  • microbiota–host interaction
  • feed additive
  • antibiotic resistance
  • animal health
  • growth performance
  • meat quality

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

20 pages, 825 KB  
Article
A Probiotic Bacillus velezensis Consortium Exhibits Superior Efficacy over Two Alternative Probiotics in Suppressing Swine Pathogens and Modulating Intestinal Barrier Function and Immune Responses In Vitro
by Josh Walker, Katrine Bie Larsen, Steffen Yde Bak, Niels Cristensen, Nicolas Chubbs, Weiqing Zeng, Adrian Schwarzenberg and Chong Shen
Microorganisms 2026, 14(1), 249; https://doi.org/10.3390/microorganisms14010249 - 21 Jan 2026
Viewed by 644
Abstract
Despite increasing interest in probiotics as antibiotic alternatives in swine production, few studies have directly compared the functional efficacy of different commercial probiotic formulations under controlled conditions. We conducted an in vitro study using porcine intestinal epithelial (IPEC-J2) and macrophage-like (3D4/21) cell models [...] Read more.
Despite increasing interest in probiotics as antibiotic alternatives in swine production, few studies have directly compared the functional efficacy of different commercial probiotic formulations under controlled conditions. We conducted an in vitro study using porcine intestinal epithelial (IPEC-J2) and macrophage-like (3D4/21) cell models to compare the efficacy of three commercial probiotic consortia (C1: three strains of Bacillus velezensis; C2: B. licheniformis + B. subtilis; C3: Clostridium butyricum). Treatments were evaluated for their ability to inhibit pathogenic Escherichia coli, Clostridium perfringens, and Salmonella spp., enhance epithelial barrier integrity, and modulate immune responses. Experimental endpoints included pathogen inhibition assays, adhesion to IPEC-J2 cells, transepithelial electrical resistance (TEER), tight junction protein expression, and cytokine profiling via RT-qPCR and proteomics. Data were analyzed using the Kruskal–Wallis test with false discovery rate (FDR) control at 5%. C1 cell-free supernatant (CFS) strongly inhibited pathogen growth (84.8 ± 5.3% inhibition of ETEC F4+F18 vs. medium control; p < 0.05), whereas C2 had no effect, and C3 inhibited only one isolate. The coculture of IPEC-J2 cells with C1 CFS increased the expression of TJ proteins ZO-1, MUC13, and MUC20 (+12.9–46.6% vs. control; p < 0.001) and anti-inflammatory TGF-β; reduced pro-inflammatory IL-6 in LPS-stimulated 3D4/21 cells. In comparison, C2 and C3 showed minimal impact on epithelial barrier integrity and immune modulation, as indicated by negligible changes in TEER values, tight junction protein expression (ZO-1, MUC13, MUC20), and cytokine profiles relative to the control. In conclusion, C1 demonstrated greater in vitro efficacy than C2 (B. licheniformis + B. subtilis) and C3 (Clostridium butyricum), including pathogen inhibition assays, epithelial adhesion, TEER measurements, and cytokine modulation, suggesting its potential as a leading candidate for functional probiotic applications. Full article
(This article belongs to the Special Issue The Role of Probiotics in Animal Health)
Show Figures

Figure 1

Review

Jump to: Research

26 pages, 926 KB  
Review
Fermentation Technology in Aquafeeds: A Review
by Haoxuan Sun, Linwei Nie, Miaoqin Huang, Weiguang Zou, Yu Hu, Xuan Luo, Weiwei You and Caihuan Ke
Microorganisms 2026, 14(2), 416; https://doi.org/10.3390/microorganisms14020416 - 10 Feb 2026
Cited by 1 | Viewed by 1108
Abstract
Fermented feed, as a novel type of functional feed, enhances the nutritional value and palatability of feed through microbial fermentation. It effectively mitigates issues such as impaired digestive function, compromised immune response in aquaculture animals, and the over-reliance on fishmeal-based diets. This paper [...] Read more.
Fermented feed, as a novel type of functional feed, enhances the nutritional value and palatability of feed through microbial fermentation. It effectively mitigates issues such as impaired digestive function, compromised immune response in aquaculture animals, and the over-reliance on fishmeal-based diets. This paper provides a narrative review of the fermented feed, its benefits, its common types, and the factors influencing its efficacy. Furthermore, it summarizes its beneficial effects in aquaculture animals, including growth promotion, immune enhancement, optimization of intestinal microbiota, and improvement in product quality. Despite its considerable potential to reduce costs and improve efficiency, fermented feed faces challenges such as limited industrial-scale production and little research on crustaceans. Future research should focus on database development, intelligent manufacturing processes, and comprehensive economic evaluations to facilitate its broader application in aquaculture. Full article
(This article belongs to the Special Issue The Role of Probiotics in Animal Health)
Show Figures

Figure 1

Back to TopTop