Search Results (17)

The bioavailability of heavy metals is profoundly influenced by their interactions with active soil components (microorganisms, organic matter, and iron minerals). However, the effects of urease-producing bacteria combined with organo-Fe hydroxide coprecipitates (OFCs) on Cd accumulation in wheat, as well as the mechanisms underlying these effects, remain unclear. In this study, pot experiments integrated with high-throughput sequencing were employed to investigate the impacts of the urease-producing bacterial strain TJ6, ferrihydrite (Fh), and OFCs on Cd enrichment in wheat grains, alongside the underlying soil–microbial mechanisms. The results demonstrate that the strain TJ6-Fh/OFC consortium significantly (p < 0.05) reduced (50.1–66.7%) the bioavailable Cd content in rhizosphere soil while increasing residual Cd fractions, thereby decreasing (77.4%) Cd accumulation in grains. The combined amendments elevated rhizosphere pH (7.35), iron oxide content, and electrical conductivity while reducing (14.5–21.1%) dissolved organic carbon levels. These changes enhanced soil-colloid-mediated Cd immobilization and reduced Cd mobility. Notably, the NH₄⁺ content and NH₄⁺/NO₃⁻ ratio were significantly (p < 0.05) increased, attributed to the ureolytic activity of TJ6, which concurrently alkalinized the soil and inhibited Cd uptake via competitive ion channel interactions. Furthermore, the relative abundance of functional bacterial taxa (Proteobacteria, Gemmatimonadota, Enterobacter, Rhodanobacter, Massilia, Nocardioides, and Arthrobacter) was markedly increased in the rhizosphere soil. These microbes exhibited enhanced abilities to produce extracellular polymeric substances, induce phosphate precipitation, facilitate biosorption, and promote nutrient (C/N) cycling, synergizing with the amendments to immobilize Cd. This study for the first time analyzed the effect and soil science mechanism of urease-producing bacteria combined with OFCs in blocking wheat’s absorption of Cd. Moreover, this study provides foundational insights and a practical framework for the remediation of Cd-contaminated wheat fields through microbial–organic–mineral collaborative strategies. Full article

In order to prevent structural damage or high repair costs caused by concrete crack propagation, the use of microbial-induced CaCO₃ precipitation to repair concrete cracks has been a hot topic in recent years. However, due to environmental constraints such as oxygen concentration, the width and depth of repaired cracks are seriously insufficient, which affects the further development of microbial self-healing agents. In this paper, a ternary microbial self-healing agent composed of different proportions of Bacillus pasteurii, Saccharomyces cerevisiae, and Bacillus mucilaginosus was designed, and its crack repair ability was evaluated. When the mixing ratio was 7:1:2, the cell concentration was the highest, the precipitation amount of CaCO₃ was the highest, and the crystallinity of calcite crystal was the highest. Compared to the single microorganism, the mortar specimens with ternary microorganisms had the largest repair area (up to 100%) and the deepest repair depth (CaCO₃ presents at 9–12 mm from the crack surface). This is because when the concrete breaks, all three microorganisms are activated by water, O_2, and CO₂. Saccharomyces cerevisiae and Bacillus mucilaginosus accelerated the growth of Bacillus pasteurii and more mineralized products; CaCO₃ was rapidly formed and quickly filled on the crack surface. When CaCO₃ seals the surface of the crack, the internal Saccharomyces cerevisiae and Bacillus mucilaginosus continue to play a role. Bacillus mucilaginosus can accelerate the dissolution of CO₂ produced by the anaerobic fermentation of Saccharomyces cerevisiae and the hydrolysis of CO₃²⁻, thereby improving the repair of the crack depth direction. Full article

The aim of the present review is to discuss the roles of vitamin K (phylloquinone or menaquinones) and vitamin K-dependent proteins, and the combined action of the vitamins K and D, for the maintenance of bone health. The most relevant vitamin K-dependent proteins in this respect are osteocalcin and matrix Gla-protein (MGP). When carboxylated, these proteins appear to have the ability to chelate and import calcium from the blood to the bone, thereby reducing the risk of osteoporosis. Carboxylated osteocalcin appears to contribute directly to bone quality and strength. An adequate vitamin K status is required for the carboxylation of MGP and osteocalcin. In addition, vitamin K acts on bone metabolism by other mechanisms, such as menaquinone 4 acting as a ligand for the nuclear steroid and xenobiotic receptor (SXR). In this narrative review, we examine the evidence for increased bone mineralization through the dietary adequacy of vitamin K. Summarizing the evidence for a synergistic effect of vitamin K and vitamin D3, we find that an adequate supply of vitamin K, on top of an optimal vitamin D status, seems to add to the benefit of maintaining bone health. More research related to synergism and the possible mechanisms of vitamins D3 and K interaction in bone health is needed. Full article

Microorganisms play a pivotal role in transforming and making phosphorus (P) available in soil through various mechanisms. However, their specific contributions to alleviating P limitation and enhancing P utilization efficiency in plants within the context of a P-deficient karst ecosystem remains unclear. In this study, eco-stoichiometric methods were employed to evaluate the P utilization efficiency of plants grown in the surveyed karst forest located in Guizhou Province, China. Metagenomic sequencing was utilized to further explore the functional genes and microorganisms involved in soil P cycling. The N:P ratio for 18 out of the 20 surveyed plants exceeded 16, indicating widespread P limitation in karst plants. Among them, plants with high P utilization efficiencies (Nandina domestica Thunb.; Mahonia bodinieri Gagnep.; Pyracantha fortuneana (Maxim.) Li) exhibited higher relative abundances of genes involved in soil P cycling compared to plants with low P utilization efficiencies (Tirpitzia sinensis (Hemsl.) Hallier f.; Albizia kalkora (Roxb.) Prain; Morella rubra Lour.), indicating greater potentials within their rhizosphere microbiomes for soil P transformation. The relative abundance of these functional genes had a significant and positive effect on plant P utilization efficiencies. Structural equation modeling further indicated that microbial P cycling gene abundance directly drove the increase in plant P utilization efficiencies. Specifically, genes involved in soil organic P mineralization (G6PD, suhB, phoD, ppx) and the P uptake and transform system (pstS, pstA, pstB, pstC) contributed to the enhancement of plant P utilization efficiencies. Soil microbial communities involved in P cycling were predominately attributed to Proteobacteria (45.16%–60.02%), Actinobacteria (9.45%–25.23%), and Acidobacteria (5.90%–9.85%), although their contributions varied among different plants. The rhizosphere functional microbial community can thus alleviate P limitation in karst plants, thereby enhancing plant P utilization efficiencies. This study investigated the strong synergism between karst plants and rhizosphere microorganisms and their associated underlying mechanisms from genetic and microbial perspectives. Full article

Anthocyanins (ACNs) have attracted considerable attention for their potential to modulate the immune system. Research has revealed their antioxidant and anti-inflammatory properties, which play a crucial role in immune regulation by influencing key immune cells, such as lymphocytes, macrophages, and dendritic cells. Moreover, ACNs contribute towards maintaining a balance between proinflammatory and anti-inflammatory cytokines, thus promoting immune health. Beyond their direct effects on immune cells, ACNs significantly impact gut health and the microbiota, essential factors in immune regulation. Emerging evidence suggests that they positively influence the composition of the gut microbiome, enhancing their immunomodulatory effects. Furthermore, these compounds synergize with other bioactive substances, such as vitamins and minerals, further enhancing their potential as immune-supporting dietary supplements. However, detailed clinical studies must fully validate these findings and determine safe dosages across varied populations. Incorporating these natural compounds into functional foods or supplements could revolutionize the management of immune-related conditions. Personalized nutrition and healthcare strategies may be developed to enhance overall well-being and immune resilience by fully understanding the mechanisms underlying the actions of their components. Recent advancements in delivery methods have focused on improving the bioavailability and effectiveness of ACNs, providing promising avenues for future applications. Full article

The present work demonstrates the novel composition of nanoparticles (NPs) of polyaniline (PANI) solo and, in combination with particles of polytetrafluoroethylene (PTFE) ~230 nm, as a powerful additive (antiwear-AWA and extreme-pressure additive EPA) in lubricating oils. The concentration of PANI NPs varied from 1–4 wt.% in a base oil and commercial 5W30 engine oil. The tribo-performance was evaluated on a four-ball tester. The PANI-based oils significantly enhanced the load-bearing ability, and 3 wt.% of PANI NPs led to enhancement in EP properties by 220% in a base oil and 58% in engine oil. Additionally, hybrid combinations of NPs of PTFE with PANI in base oil were prepared by mixing in a ratio of 3:1 and 2:1 and were explored for possible tribo-synergism in EP properties. The hybrid nano-oils led to the highest reported ~ 535% enhancement in the load-carrying capacity of mineral oil. The lubrication mechanisms for enhanced tribo performance were linked with studies on a scanning electron microscope, an energy-dispersive X-ray analyzer, and with the use of Raman spectroscopy. Full article

Carbonated water has proven advantages over conventional CO₂ injection in terms of arresting free CO₂ mobility, low-pressure injection, lower volume requirement, and higher efficiency. The term “engineered water” is designated to selective ion-spiked injection water with the advantage of the ion-exchange reactions with the rock minerals and releasing trapped oil. This article investigated the synergic effect of dissolved CO₂ and engineered water for oil recovery and understanding inner mechanisms. Recovery efficiencies were evaluated through coreflood studies, which revealed that the hybrid water could recover 6–10% more oil than engineered water and about 3% more than carbonated water. HP-HT pendant-drop studies show the insignificance of IFT reduction. Wettability change from oil wet to near-water wet is attributed as a significant factor. The dissolution of Ca²⁺ and Mg²⁺ and deposition of SO₄²⁻ observed in coreflooding may have a significant contribution to oil recovery. Pore enlargement evidenced in NMR-PSD and NMR-ICP results support this claim. The study confirmed that the EWI-CWI hybrid technique could be a promising EOR method, eliminating the requirement for high-pressure injection, the problems of gravity segregation, and the early breakthrough of CO₂. It can also be an effective EOR solution, providing a significant cost advantage and higher oil recovery in addition to the environmental benefits of CO₂ sequestration. Full article

This paper aims at reducing greenhouse gas emissions, which contributes to carbon neutrality, and, at the same time, preventing mine heat disasters and extracting highly mineralized (HM) mine water, so as to realize the synergy between CO₂ storage (CS) and geothermal extraction and utilization (GEU) in a high temperature (HT) goaf. With this purpose, an innovative CS-GEU technology for HT and HM water in deep mine is proposed, based on the mechanism of water-rock-CO₂ effect (WRCE) and the principle of GEU in the mine. This technology uses GEU to offset the costs of CO₂ storage and refrigeration in HT mine. A general scheme for a synergistic system of CS and GEU in the goaf is designed. The feasibility of CS-GEU technology in the deep goaf is demonstrated from the views of CS and GEU in the goaf and the principles of a synergistic system. It is clarified that the CO₂ migration-storage evolution and the multi-field coupling principle in the goaf are the key scientific issues in realizing the synergic operation of CS and GEU. It proposes the key techniques involved in this process: CO₂ capture and CO₂ transportation, layout and support of drill holes and high-pressure (HP) pipelines, and HP sealing in the goaf. The research results provide new ideas for CS and GEU of HT and HM mine water in deep mine. Full article

The silt in the Yellow River alluvial plain typically features low strength and poor water stability, and, thus, alluvial silt treatment needs an amount of cement to improve soil performance. The development of an alternative to reduce or replace the use of cement in soil stabilization has been a hot topic research for a long time. This paper develops an industrial-solid-waste (ISW) curing agent using a response surface methodology, which is a novel composite material made of steel slag, mineral slag, and two desulfurization products; its feasibility on improved silt is expected to be studied systematically. The comparative tests of ISW- and cement-improved silt were conducted to analyze performance and action mechanism. Variance and multiple regression analysis were used to study the effect of factors on responses statistically, and check the significance and correlation of the suggested models. Finally, the in-service performance of ISW-improved silt was evaluated through in-situ tests. Results show that ISW-improved silt can present good mechanical properties and durability, but is much weaker than cement-improved silt in the early curing stage. The strength enhancement amplitude of ISW-improved silt between curing ages of 7 days to 28 days is larger than that of cement-improved silt. The correlation between factors and responses is established with good agreement. Synergisms in the ISW curing agent are stimulated in the alkaline environment, and are conductive to connect the silt particles. The in-service performance of ISW-improved silt showed little difference to that of cement-improved silt; both of them meet the requirements from the perspective of in-situ application. Moreover, the unit cost of an ISW curing agent is less than 1/5 of that of cement. ISW-improved silt has advantages of cost saving, resource recycling and environmental protection. Full article

Graphitic carbon nitride (g-C₃N₄) was used to enhance the photocatalytic activity of ZnO nanoparticles for the degradation of 5-fluorouracil (5-FU) cytostatic drug under UV-LED irradiation. CN/ZnO composites were synthetized by an easy one-pot thermal method, varying the g-C₃N₄ loading, i.e., from 10 to 67 wt% and a post-thermal exfoliation in air. The physicochemical and optical properties of the materials were analyzed by several techniques. CN/ZnO composites showed a coral-like structure of spherical ZnO wurtzite particles on the g-C₃N₄ structure. In general, the synergism and heterojunction interface between both phases allowed the enhancement of the mesoporosity, light absorption ability, and the aromaticity of the corresponding composites. Moreover, the photocatalytic activity of the CN/ZnO composites was increased with the addition of g-C₃N₄ in comparison with pristine ZnO. The highest activity was found for the composite containing 25 wt% of g-C₃N₄ (i.e., CN25/ZnO), reaching the total degradation of 5-FU and a mineralization of 48% at 180 min, as well as a good photostability during four reuse cycles. Experiments with different pH solutions and scavengers allowed for the assessment of the reactive oxygen species (ROS) involved in the 5-FU degradation pathway, with radicals and non-radical species as the main responsible active species. Furthermore, a tentative photocatalytic mechanism was proposed for CN/ZnO composites. Full article

Durum wheat is one of the most commonly cultivated species in the world and represents a key commodity for many areas worldwide, as its grain is used for production of many foods, such as pasta, bread, couscous, and bourghul. Durum wheat grain has a relevant role in the human diet, providing carbohydrates, proteins, lipids, fibres, vitamins, and minerals, as well as highly valued bioactive compounds contributing to a healthy diet. Durum wheat is largely cultivated in the Mediterranean basin, where it is mainly grown under rain-fed conditions, thus currently undergoing drought stress, as well as soil salinity, which can hamper yield potential and influence the qualitative characteristics of grain. When plants suffer drought and/or salinity stress, a condition known as hyperosmotic stress is established at cellular level. This leads to the accumulation of ROS thus generating in turn an oxidative stress condition, which can ultimately result in the impairment of cellular integrity and functionality. To counteract oxidative damage due to excessive ROS production under stress, plants have evolved a complex array of both enzymatic and non-enzymatic antioxidant mechanisms, working jointly and synergically for maintenance of ROS homeostasis. Enhancement of antioxidant defence system has been demonstrated as an adaptive mechanism associated to an increased tolerance to hyperosmotic stress. In the light of these considerations, this review provides a concise overview on recent advancements regarding the role of the ascorbate-glutathione cycle and the main antioxidant enzymes (superoxide dismutase, catalase, and peroxidases) in durum wheat response to drought and salt stresses that are expected to become more and more frequent due to the ongoing climate changes. Full article

Infection with pathogenic microorganisms is of great concern in many areas, especially in healthcare, but also in food packaging and storage, or in water purification systems. Antimicrobial polymer nanocomposites have gained great popularity in these areas. Therefore, this study focused on new approaches to develop thin antimicrobial films based on biodegradable polycaprolactone (PCL) with clay mineral natural vermiculite as a carrier for antimicrobial compounds, where the active organic antimicrobial component is antifungal ciclopirox olamine (CPX). For possible synergistic effects, a sample in combination with the inorganic antimicrobial active ingredient zinc oxide was also prepared. The structures of all the prepared samples were studied by X-ray diffraction, FTIR analysis and, predominantly, by SEM. The very different structure properties of the prepared nanofillers had a fundamental influence on the final structural arrangement of thin PCL nanocomposite films as well as on their mechanical, thermal, and surface properties. As sample PCL/ZnOVER_CPX possessed the best results for antimicrobial activity against examined microbial strains, the synergic effect of CPX and ZnO combination on antimicrobial activity was proved, but on the other hand, its mechanical resistance was the lowest. Full article

Advanced oxidation (e.g., fenton-like reagent oxidation and ozone oxidation) is a highly important technology that uses strong oxidizing free radicals to degrade organic pollutants and mineralize them. The fenton-like reactions have the characteristics of low cost, simple operation, thorough reaction and no secondary pollution. Fenton-like reagents refer to a strong oxidation system composed of transition metal ions (e.g., Fe³⁺, Mn²⁺ and Ag⁺) and oxidants (hydrogen peroxide, potassium persulfate, sodium persulfate, etc). Graphene and carbon nanotube possess a distinctive mechanical strength, flexibility, electrical and thermal conductivity and a very large specific surface area, which can work as an excellent carrier to disperse the catalyst and prevent its agglomeration. Fullerene can synergize with iron-based materials to promote the reaction of hydroxyl groups with organic pollutants and enhance the catalytic effect. Fenton-like catalysts influence the catalytic behavior by inducing electron transfer under strong interactions with the support. Due to the short lifespan of free radicals, the treatment effect is usually enhanced with the assistance of external conditions (ultraviolet and electric fields) to expand the application of fenton-like catalysts in water treatment. There are mainly light-fenton, electro-fenton and photoelectric-fenton methods. Fenton-like catalysts can be prepared by hydrothermal method, impregnation and coordination-precipitation approaches. The structures and properties of the catalysts are characterized by a variety of techniques, such as high-resolution transmission electron microscopy, high-angle annular dark-field scanning transmission electron microscopy and X-ray absorption near-edge structure spectroscopy. In this paper, we review the mechanisms, preparation methods, characterizations and applications status of fenton-like reagents in industrial wastewater treatment, and summarize the recycling of these catalysts and describe prospects for their future research directions. Full article

Among several ions playing a vital role in the body, Sr²⁺ and Mg²⁺ are involved in the mechanism of bone formation, making them especially useful for bone tissue engineering applications. Recently, polylactic acid (PLA)/Mg composites have emerged as a promising family of biomaterials due to their inherent biocompatibility and biodegradability properties. In these composites, polymer and bio-metal have a synergetic effect—while the PLA inhibits the Mg fast reactivity, Mg provides bioactivity to the inert polymer buffering the medium pH during degradation. Meanwhile, the typical form of administrating Sr²⁺ to patients is through the medication strontium ranelate (SrR), which increases the bone mineral density. Following this interesting research line, a new group of composites, which integrates Mg particles and SrR charged onto halloysite nanotubes (HNT) in a polymeric matrix, was proposed. PLA/Mg/SrR–HNT composites have been processed following a colloidal route, obtaining homogenous composites granulated and film-shaped. The drug delivery profile was evaluated in terms of in vitro lixiviation/dissolution paying special attention to the synergism of both ions release. The combination of two of the most reported ions involved in bone regeneration in the composite biomaterial may generate extra interest in bone healing applications. Full article

Storms and wind damage are the main cause of biomass loss in forests of Northern Europe, as well as they are synergic with the disturbances causing intense water and temperature stress. This highlights the necessity for climate-smart management at landscape level coupling ecological demands of forestry species with their wind resistance. Silver birch (Betula pendula Roth.), which is highly plastic species, appears to be promising for a wider application under such conditions, as it is believed to tolerate wide range of weather conditions. Though silver birch can be sensitive to water deficit and windthrow, local information on its wind tolerance in sites with different moisture regimes is advantageous. Mechanical stability of 71 mid-aged silver birches (Betula pendula Roth.) growing in seven dry (Hylocomiosa) and five periodically waterlogged (Myrtilloso-sphagnosa) sites with mineral soils in Latvia (hemiboreal lowland conditions) were assessed by the destructive static pulling tests. Site type had a significant, yet intermediate effect on the stability of silver birch. As expected, trees under periodically waterlogged conditions were more prone to collapse under static loading, however, they showed a better resistance to primary failure (beginning of wood structure deformation). Uprooting was the most common form of tree collapse. Surprisingly, considering similar root depths, stem breakage was more frequent in the periodically waterlogged than dry sites (21.9 vs. 5.1%, respectively), indicating high loading resistance of roots, supporting high plasticity and wind resistance of the studied metapopulation of silver birch. Nevertheless, in the periodically waterlogged sites, the difference between forces needed to cause primary and secondary (collapse) failures of stem decreased with age/size, implying necessity for optimization of rotation length. Accordingly, quantification of wind resistance can aid climate-smart selection of species for forest regeneration depending on landscape, suggesting birch as wind resistant option under periodically waterlogged conditions. Full article