Search Results (215)

The growing need for sustainable protein and functional food ingredients has made edible insects stand out as a flexible source of bioactives. Black Soldier Fly larva (BSFL) bioactives, such as chitooligosaccharides (COSs) and peptides, present potential benefits for gut health; nevertheless, their molecular pathways, clinical validation, and commercial scalability have yet to be thoroughly investigated. This study systematically analyzes current progress in BSFL bioactive extraction and characterization, emphasizing enzymatic and thermal processing, controlled enzyme development, and integrated supercritical fluid enzymatic pipelines. We assess preclinical and animal research that illustrates prebiotic modulation of Bifidobacterium, Lactobacillus, and Faecalibacterium populations; antimicrobial peptide-mediated immune signaling; and antioxidant activity. Multi-omics frameworks that connect the microbial metabolism of COS to gut health help us understand how these processes function. A comparison of the regulatory environments for food and feed applications in the EU, North America, and Asia shows that there are gaps in human safety trials, harmonized standards, and techno-economic assessments. Finally, we suggest some next steps: randomized controlled human trials in groups with irritable bowel syndrome (IBS) and metabolic syndrome; standardized data integration pipelines for multi-omics; and life cycle and cost–benefit analyses of modular, vertically integrated BSFL biorefineries with AI-driven reactors, digital twins, and blockchain traceability. Addressing these issues will hasten the conversion of BSFL bioactives into safe, effective, and sustainable functional meals and nutraceuticals. Full article

With the increasing global demand for energy, the development of unconventional resources has become a focal point of research. Among these, shale gas has drawn considerable attention due to its abundant reserves. However, its low permeability and complex fracture networks present substantial challenges. This study investigates the composite fracturing technology combining supercritical CO₂ and slickwater for shale gas extraction, elucidating the mechanisms by which it influences shale fracture roughness and conductivity through an integrated approach of theory, experiments, and numerical modeling. Experimental results demonstrate that the surface roughness of shale fractures increases markedly after supercritical CO₂–slickwater treatment. Moreover, the dynamic evolution of permeability and porosity is governed by roughness strain, adsorption expansion, and corrosion compression strain. Based on fluid–solid coupling theory, a mathematical model was developed and validated via numerical simulations. Sensitivity analysis reveals that fracture density and permeability have a pronounced impact on shale gas field productivity, whereas fracture dip angle exerts a comparatively minor effect. The findings provide a theoretical basis for optimizing composite fracturing technology, thereby enhancing shale gas extraction efficiency and promoting effective resource utilization. Full article

Hybrid polysaccharide-based aerogels offer significant potential as advanced drug delivery platforms due to their tunable structure, high porosity, and biocompatibility. In this study, aerogel microparticles were synthesized using alginate, pectin, carrageenan, and their hybrid formulations via an emulsion–gelation technique followed by supercritical fluid CO₂ extraction. The resulting aerogels exhibit mesoporous structures with specific surface areas ranging from 324 to 521 m²/g and pore volumes between 1.99 and 3.75 cm³/g. Comprehensive characterization (SEM, gas sorption, XRD, TGA, DSC, and FTIR) confirmed that hybridization improved morphological uniformity and thermal stability compared to single polymer aerogels. Ibuprofen was used as a model drug to evaluate loading efficiency and release kinetics. Among all formulations, the alginate/carrageenan (2:1) hybrid showed the highest drug loading efficiency (93.5%) and a rapid release profile (>90% within 15 min), closely matching the performance of commercial ibuprofen tablets. Drug release followed Fickian diffusion, as confirmed by the Korsmeyer–Peppas model (R² > 0.99). These results highlight the potential of hybrid polysaccharide aerogels as vehicles for drug delivery and other fast-acting therapeutic applications. Full article

Olive leaves, the main byproducts of olive cultivation, are characterized by a plethora of bioactive metabolites with significant nutritional value. Their main pentacyclic triterpenes, Oleanolic Acid (OA) and Maslinic Acid (MA), are two high added-value compounds with remarkable activities. This study aimed to develop an efficient methodology for extracting and purifying OA and MA, utilizing Supercritical Fluid Extraction (SFE) and Centrifugal Partition Chromatography (CPC)—two modern, scalable, and green techniques. A total of 21 g of olive leaves were subjected to SFE using supercritical CO₂ and ethanol as co-solvent. The extraction employed a step gradient mode, starting with 100% CO₂ and incrementally increasing ethanol (0–10% w/w) every 20 min. Fractions rich in OA and MA (500 mg) were further purified via CPC, utilizing pH zone refining to exploit the protonation and deprotonation properties of acidic triterpenes. The biphasic solvent system consisted of n-hexane, ethyl acetate, ethanol, and water (8:2:5:5 v/v/v/v), with trifluoroacetic acid added to the stationary phase and triethylamine added to the mobile phase. This two-step process yielded 89.5 mg of OA and 28.5 mg of MA with over 95% purity, as confirmed by HPLC-ELSD and ¹H-NMR. Moreover, purified compounds and SFE fractions exhibited promising elastase and collagenase inhibition, highlighting them as dermocosmetic agents. Full article

The valorization of by-products in the olive sector has increasingly become the focus of business and research in the context of biorefineries. This work evaluates the recovery of bioactive compounds from olive leaves and their subsequent incorporation into poly(L-lactic- acid-co-caprolactone) (PLCL) filaments through supercritical impregnation. Obtaining an olive leaf extract (OLE) using enhanced solvent extraction at a high pressure (ESE with CO₂/ethanol 1:1 v/v) resulted in higher yields and concentrations of bioactives with high antioxidant and anti-inflammatory activity. No significant differences were found between the extracts obtained with different water regimes (irrigated and dry land). The supercritical impregnation of PLCL filaments showed that a low depressurization rate is essential to avoid material deformation, while the impregnation pressure and temperature influenced the OLE loading and antioxidant activity of the filaments. In vitro release studies showed the prolonged release of active compounds over 90 days, and the kinetics best fit the Korsmeyer–Peppas model, suggesting a diffusion mechanism. These results validate supercritical impregnation as a promising strategy for the development of OLE-active PLCL filaments with potential for biomedical applications requiring sustained therapeutic release. Full article

Tannins are structurally diverse polyphenols prized for their antioxidant and protein-binding properties, making them valuable in leather tanning, adhesives, coatings, and water treatment. This article compares research on conventional versus supercritical fluid extraction (SFE), which is recognized as an eco-friendly and efficient technique. While SFE using supercritical CO₂ is already widely studied and commercially implemented for various botanical compounds, its application to tannin extraction remains in an earlier stage of development, with limited industrial solutions currently available. Various solvents (CO₂, water, ethanol, and methanol) and their mechanisms of action under supercritical conditions are discussed, demonstrating how adjusting parameters like pressure and temperature can selectively isolate specific tannin fractions. By reviewing multiple studies on yield, solvent choice, process efficiency, and product purity, the article highlights SFE’s advantages in preserving tannin quality while reducing energy consumption and contamination. The conclusions suggest SFE as a promising method for sustainable tannin production, offering tailored extracts that meet the growing demand for green processes in both industrial and biomedical applications. Full article

The influence of supercritical CO₂ on the properties of petroleum has become the focus of academic and industrial attention internationally. CO₂ has been shown in laboratory studies and in field applications of shale oil to be an effective oil displacement agent. In this paper, the research progress of the interaction between CO₂ and crude oil is investigated from three perspectives: (i) the research methods of the interaction experiment between CO₂ and crude oil; (ii) the influence of CO₂ on oil property and the primary controlling factors; and (iii) the cause, influence, and harm of CO₂-induced asphaltene precipitation. Our current knowledge on this topic is as follows: (1) Physical simulation can investigate the effects of various variables on CO₂ displacement, which is in situ and intuitive. Numerical simulation can investigate the displacement principle at the microscopic molecular level and also scale up the results of physical simulation to the macroscopic scale of oilfield production to explore the long-term large-scale injection rules; (2) after entering the formation, CO₂ dissolves in crude oil, expands the volume of crude oil, reduces the viscosity, improves the oil–water mobility ratio, reduces the oil–water interfacial tension, and extracts light hydrocarbons to form a miscible displacement zone; (3) after CO₂ is injected into the formation and dissolves in crude oil, it occupies the surface space of asphaltenes and causes asphaltenes to precipitate. Under the combined influence of internal and external factors, the precipitation of asphaltenes has a significant impact on the physical properties of the reservoir. Clarifying the influencing factors of CO₂ on the property of crude oil has reference significance for understanding the reaction characteristics between supercritical CO₂ and formation fluids, providing a theoretical basis for CO₂ injection enhanced oil recovery technology, and has reference value for carbon storage research. Full article

Rosmarinic acid (RA) is a bioactive phenolic compound prevalent in various medicinal plants, renowned for its significant pharmacological properties. This study aims to optimise the extraction conditions of this compound from Rosmarinus officinalis L. using the response surface methodology (RSM) with a three-variable, three-level Box–Behnken design. Optimising the parameters for supercritical CO₂ (scCO₂) extraction focused on pressure (150 to 350 bar), temperature (40 to 80 °C), and co-solvent weight percentage (5 to 15% ethanol), evaluating their impact on overall yield and RA content. The optimal conditions determined were a pressure of 150 bar, a temperature of 80 °C, and 15% ethanol, yielding a total extract of 21.86 ± 1.55%, with an RA content of 3.43 ± 0.13 mg/g dry matter (DM). Scanning electron microscopy revealed that the scCO₂ treatment induced microcracks on the surface of the rosemary powder, enhancing the fluid’s ability to penetrate the plant matrix. By employing the combined scCO₂-Soxhlet method, the RA content increased to 5.78 mg/g DM. Furthermore, the final extract obtained using the Soxhlet post-scCO₂ treatment contained only trace amounts of carnosic acid (0.38 ± 0.10 mg/g DM) and carnosol (0.38 ± 0.20 mg/g DM), compared to the crude extract obtained solely with Soxhlet, which exhibited significantly higher concentrations of 8.45 ± 2.98 mg/g DM of carnosol and 16.67 ± 0.94 mg/g DM of carnosic acid. This work highlighted an innovative extraction strategy based on the coupling of scCO₂ and Soxhlet, which significantly increased RA content while reducing concentrations of other compounds such as CA and CAR. This approach makes it possible to produce RA-enriched extracts, offering considerable potential for future large-scale applications and commercialisation. Full article

Fruit processing by-products contain various classes of bioactive constituents, which may find applications as ingredients for foods, nutraceuticals or cosmeceuticals. This study explored the fractionation of lipophilic rowanberry pomace extracts isolated with pure supercritical CO₂ and its mixtures with a co-solvent ethanol by their on-line separation at subcritical conditions. Rowanberry pomace lipids were extracted with supercritical CO₂ (42.4 MPa, 53 °C) using 0–7% of ethanol, and then fractionated by reducing the first separator’s (S1) pressure to 7 MPa and cooling it to 0, −10 and −20 °C to precipitate the ‘heavier’ fraction (HF). The second separator (S2) was depressurized at ambient temperatures to collect the ‘lighter’ fraction (LF). The yield of the LF increased by decreasing the S1 temperature and increasing the amount of the co-solvent. The concentration of β-carotene was increased in the LF by decreasing the S1 temperature and increasing the co-solvent concentration; at −20 °C it was 66.7% higher than in the non-fractionated extract. The concentrations of tocopherols and phytosterols were also remarkably higher in the LF. In total, 62 compounds were identified in the headspace volatile fraction of the LF, benzaldehyde and benzyl alcohol being the most abundant constituents. In conclusion, fractionation enabled us to obtain fractions with higher concentrations of the selected classes of lipophilic rowanberry constituents. Full article

Background: Depression ranks among the most severe mental health conditions, and poses a burden on global health. Agarwood, an aromatic medicinal plant, has shown potential for improving mental symptoms. As a common folk medicine, agarwood has been applied as an alternative method for mental disorders such as depression through aromatherapy. Previous studies have found that the therapeutic effects of agarwood aromatherapy are primarily related to its volatile components. This study aimed to examine the antidepressant properties and underlying mechanisms of agarwood essential oil (AEO), a collection of the volatile components of agarwood utilized through aromatherapy inhalation and injection administration in mice. Methods: A lipopolysaccharide (LPS)-induced inflammatory depression model was used to evaluate the effects of AEO inhalation and injection on depression-like symptoms. Behavioral assessments included the open-field, tail suspension, and forced swimming tests. Western blot (WB) and ELISA techniques were used to further verify the mechanistic insights. Results: In the LPS-induced depression-like model, AEO inhalation and injection significantly improved depression-like symptoms, decreased immobility duration in both the tail suspension and forced swimming tests in model mice, and reduced the levels of inflammatory cytokines IL-1β, IL-6, and TNF-α. WB experiments demonstrated that AEO inhibited the NF-κB/IκB-α inflammatory pathway and activated the BDNF/TrkB/CREB pathway in the hippocampus of the LPS-depression model mice. Notably, AEO extracted by hydrodistillation was more effective in alleviating LPS-induced depressive-like behaviors than using supercritical CO₂ fluid extraction. Conclusions: Both the inhalation and the injection administration of AEO exerted notable antidepressant effects, potentially associated with reducing inflammation levels in the brain, downregulating inflammatory NF-κB/IκB-α, and upregulating the neuroprotective BDNF/TrkB/CREB signaling pathway. In the future, it is necessary to further determine the pharmacodynamic components, key targets and specific molecular mechanisms of AEO’s antidepressant effects so as to provide more support for the neuroprotective research of medicinal plants. Full article

In the present study, different methodologies with potential scalability and environmental friendliness, such as winterization, supercritical fluid extraction, and multistage distillation, were evaluated for lauric acid concentration. In all cases, to facilitate fractionation, the transformation of triacylglycerols into free fatty acids or fatty acid ethyl esters was required as a previous step. For the winterization experimental assays, the amount and type of solvent was studied, resulting in a product containing ~65% lauric acid with a recovery of ~81% using a 1:10 oil-to-solvent ratio with hexane. On the other hand, the experimental extraction with supercritical carbon dioxide in a counter current packed column at 55 °C, 115 bar, and 70 g CO₂/min, resulted in a product composed of ~80% lauric acid as ethyl ester with a recovery of ~85%. Finally, flash and multistage distillation were analysed using process simulation (Aspen Plus V14), demonstrating that this methodology can achieve 80% recovery with high purity (lauric acid: 96.7%; ethyl laurate: 97.4%), but a high vacuum is required to prevent thermal degradation of the product (lauric acid: 0.2 mbar; ethyl laurate: 1.1 mbar). Overall, the employed methodologies proved highly efficient in concentrating lauric acid, yielding a product of commercial interest and high added value. Full article

Understanding the behavior of carbon dioxide (CO₂) under varying thermodynamic conditions is essential for optimizing processes such as Carbon Capture and Storage (CCS) and supercritical fluid extraction. This study employs molecular dynamics (MD) simulations with the EPM2 and TraPPE-small force fields to examine CO₂ phase behavior, structural characteristics, and transport properties across a temperature range of 228–500 K and pressures from 1 to 150 atm. Our findings indicate a good agreement between simulated and experimental liquid–vapor coexistence curves, validating the capability of both force fields to model CO₂ accurately in a wide range of thermodynamical conditions. Radial distribution functions (RDFs) reveal distinct interaction patterns in liquid and supercritical phases, while mean squared displacement (MSD) analyses show diffusivity increasing from $5.2\times {10}^{-9}$ m²/s at 300 K to $1.8\times {10}^{-8}$ m²/s at 500 K. Additionally, response functions such as the heat capacity effectively capture phase transitions. These findings provide quantitative insights into CO₂ phase behavior and transport properties, enhancing the predictive reliability of simulations for CCS and related industrial technologies. This work bridges gaps in the CO₂ modeling literature and highlights the potential of MD simulations in advancing sustainable applications. Full article

Propolis is a valuable natural resource for extracting various beneficial compounds. This study explores a sustainable extraction approach for Brazilian green propolis. First, supercritical fluid extraction (SFE) process parameters were optimized (co-solvent: 21.11% v/v CPME, and temperature: 60 °C) to maximize yield, total phenolic content (TPC), antioxidant capacity, and LOX (lipoxygenase) inhibitory activity. GC–MS analysis identified 40 metabolites in SFE extracts, including fatty acids, terpenoids, phenolics, and sterols. After selecting the optimum SFE process parameters, a sequential high-pressure extraction (HPE) approach was developed, comprising SFE, pressurized liquid extraction (PLE) with EtOH/H₂O, and subcritical water extraction (SWE). This process was compared to a similar sequential extraction using low-pressure extractions (LPE) with a Soxhlet extractor. The HPE process achieved a significantly higher overall yield (80.86%) than LPE (71.43%). SFE showed higher selectivity, resulting in a lower carbohydrate content in the non-polar fraction, and PLE extracted nearly twice the protein amount of LPE–2. Despite the HPE selectivity, LPE extracts exhibited better acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and LOX inhibition, demonstrating that the neuroprotective and anti-inflammatory activity of the extracts may be associated with a symbiosis of a set of compounds. Finally, a comprehensive greenness assessment revealed that the HPE process proved more sustainable and aligned with green chemistry principles than the LPE method. Full article

Green chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from Eucalyptus marginata leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction times, high energy consumption, and potential toxic solvent residues. This study explored the use of supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and gas-expanded liquid (GXL) processes to improve efficiency and selectivity. These techniques were combined in a single mixture design, where CO₂ was used in the experiments carried out under SFE, while water and ethanol were used for the PLE and GXL experiments by varying the concentration of the solvents to cover all the extraction possibilities. The neuroprotective activity of the extracts was evaluated by measuring their antioxidant, anti-inflammatory, and acetylcholinesterase inhibition properties. The optimization resulted in a novel GXL extraction with an optimal ternary mixture of 27% CO₂, 55% ethanol, and 18% water, with a high degree of desirability (R² = 88.59%). Chromatographic analysis carried out by GC-MS and HPLC-ESI-MS/MS identified over 49 metabolites. The designed sustainable extraction process offers a promising approach for producing phenolic-rich plant extracts in industrial applications. Full article

Sustainable management of agri-food product safety presents a major challenge requiring extensive action to ensure food safety and consumer health. The pursuit of environmentally friendly solutions that will constitute an alternative to the chemical compounds commonly used in agriculture and the food industries is one of the most important problems. One solution is plant extracts containing various biologically active compounds and exhibiting antimicrobial activity. This study aims to determine the biological activity of extracts obtained from Origanum vulgare L. (leaves) by supercritical CO₂ (SC-CO₂) extraction using different reaction conditions and compositions. In vitro studies revealed antimicrobial activity against selected bacteria (including Salmonella Enteritidis, Listeria monocytogenes, and Staphylococcus aureus) and fungi (Fusarium spp.), depending mainly on the microorganism species; however, extraction conditions also influenced these properties. The microscopic observations established by optical and fluorescence microscopy showed the changes in the fungal cell’s viability and morphology. There was no observed significant release of intracellular material as stated based on ICP-MS analysis of sodium and potassium concentration. Antibiofilm properties of extract obtained by extraction at 40 °C were also demonstrated against S. aureus, P. aeruginosa, and L. monocytogenes, with stronger properties observed against Gram-positive bacteria. Phytochemical characterization of the extracts was determined using a liquid chromatography system with an orbitrap mass spectrometer (LC/MS), identifying, i.e., phenolic acids: protocatechuic, hydroxybenzoic, caffeic, and rosmarinic; flavonoids: luteolin, naringenin, and kaempferol; and terpenoids: oleanolic and ursolic acids. Full article