Search Results (991)

The interaction between microorganisms in the dental microfilm (plaque) at the gingival margin, the immune system, and the brain is vital for gingival health. The brain constantly receives information regarding microbial composition and inflammation status through afferent nerves and the bloodstream. It modulates immune responses via efferent nerves and hormonal systems to maintain homeostasis. This relationship determines whether the gingiva remains healthy or develops into gingivitis (non-destructive inflammation) or periodontitis (a destructive condition), collectively referred to as periodontal disease. Factors associated with severe periodontitis heighten the responsiveness of this homeostatic system, diminishing the adaptive immune system’s defence against symbiotic microorganisms with pathogenic properties, known as pathobionts. This leads to excessive innate immune system activation, effectively preventing infection but damaging the periodontium. Consequently, investigating the microbiota–brain axis is vital for understanding its impact on periodontal health and disease. Herein, we examine recent advancements in how the defence against pathobionts is organised within the brain, and how it regulates and adapts the pro-inflammatory and anti-inflammatory immune balance, controlling microbiota composition. It also discussed how pathobionts and emotional stress can trigger neurodegenerative diseases, and how inadequate coping strategies for managing daily stress and shift work can disrupt brain circuits linked to immune regulation, weakening the adaptive immune response against pathobionts. Full article

The Mongolian wild ass (Equus hemionus hemionus) is a flagship species of the desert-steppe ecosystem in Asia, and understanding its strategies for coping with cold environments is vital for both revealing its survival mechanisms and informing conservation efforts. In this study, we employed metagenomic sequencing to characterize the composition and functional potential of the gut microbiota, and applied DNA metabarcoding of the chloroplast trnL (UAA) g–h fragment to analyze dietary composition, aiming to reveal seasonal variations and the interplay between dietary plant composition and gut microbial communities. In the cold season, Bacteroidota and Euryarchaeota were significantly enriched, suggesting enhanced fiber degradation and energy extraction from low-quality forage. Moreover, genera such as Bacteroides and Alistipes were also significantly enriched and associated with short-chain fatty acid (SCFA) metabolism, bile acid tolerance, and immune modulation. In the cold season, higher Simpson index values and tighter principal coordinates analysis (PCoA) clustering indicated a more diverse and stable microbiota under harsh environmental conditions, which may represent an important microecological strategy for the host to cope with extreme environments. Functional predictions based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) further indicated upregulation of metabolic and signaling pathways, including ABC transporters, two-component systems, and quorum sensing, suggesting multi-level microbial responses to low temperatures and nutritional stress. trnL-based plant composition analysis indicated seasonal shifts, with Tamaricaceae detected more in the warm season and Poaceae, Chenopodiaceae, and Amaryllidaceae detected more in the cold season. Correlation analyses revealed that dominant microbial phyla were associated with the degradation of fiber, polysaccharides, and plant secondary metabolites, which may help maintain host energy and metabolic homeostasis. Despite the limited sample size and cross-sectional design, our findings highlight that gut microbial composition and structure may be important for host adaptation to cold environments and may also serve as a useful reference for future studies on the adaptive mechanisms and conservation strategies of endangered herbivores, including the Mongolian wild ass. Full article

Given the endangered status and very limited distribution of Barbarea vulgaris R.Br. subsp. lepuznica (Nyár.) Soó in stressful, high-elevation habitats, where these plants must prioritise the resource acquisition and vegetative growth to sustain their survival and persistence, we aimed to reveal possible abiotic/biotic-driven constraints in biomass allocation for flower production. Three functional traits, i.e., the tallest shoot height, leaf mass area (LMA) and number of inflorescences (racemes), were measured in thirty plants in each of the three studied populations differing in altitude and sheep grazing intensity (P1—1700 m, grazed; P2—1900 m, ungrazed; P3—2100 m, ungrazed). The LMA and dominant shoot height were significantly higher and, respectively, lower in P3 compared with P1. Although the mean number of racemes in P1 was lower than in P2 and P3, the differences were not statistically significant. The tallest shoot height, followed by the LMA, displayed the highest contribution to differentiating the three populations. The raceme count decreased significantly with increasing height of the dominant shoot in P1 and P2, and also with increasing LMA in P3. The observed constraint in raceme production within all populations is very likely one facet of the trade-off between reproductive and vegetative allocation under harsh edapho-climatic conditions. The studied plants have adopted a conservative-tolerant strategy to cope with the abiotic stress at higher elevations, but an acquisitive-tolerant strategy in face of grazing. The subspecies lepuznica seems to be in a favourable conservation status, but a close monitoring in grazed areas is recommended. Full article

Background/Objectives: Home healthcare assistants (HHCAs) play a vital role in supporting older adults to remain in their homes. Yet, this work is often performed under conditions of emotional strain, limited resources, and systemic undervaluation. This study answers the question, how do HHCAs cope with work-related stress? Methods: Undertaken during the COVID-19 pandemic, a period of heightened stress and mandated social distancing, online interviews were conducted with HHCAs (n = 10). Data were inductively analyzed and themes were identified. Results: It was found that amid experiences of fear, caregiver stress, grief, and exhaustion, HHCAs coped with resource, communication, and care challenges by relying on informal peer-managed communication systems with colleagues. Leveraging existing peer-support coping strategies, HHCAs negotiated caring for others while taking care of themselves alongside a care ecosystem under unprecedented strain. Conclusions: HHCAs are increasingly vital to front-line home health care amid global aging and a shift toward community-based services. Urgent organizational reform is needed to support their well-being, prevent stress, and avoid burnout. Research-informed sector-wide planning must ensure adequate resources to maintain high-quality home care in the face of rising demand and anticipated future health crises. Full article

Background: Bruxism is a multifactorial biopsychosocial condition involving repetitive jaw muscle activity, influenced by psychological stress, anxiety, and maladaptive coping strategies. Methods: In this cross-sectional observational study, 111 health sciences students (mean age 22.29 ± 3.10 years; 66.6% women) underwent standardized DC/TMD clinical evaluation and completed the GAD-7, COPE Inventory, and a 105-item Stroop Test. Results: Bruxism prevalence was 63.96% (71/111). Students with bruxism had higher GAD-7 scores (10.63 ± 5.78 vs. 5.80 ± 3.66; mean difference 4.83, 95% CI 2.88–6.78; p < 0.001, g = 0.94), with clinically relevant anxiety nearly three times more frequent (55.4% vs. 19.6%; RR = 2.83, 95% CI 1.68–4.76). Stroop interference times were slower in the bruxism group (42.19 ± 16.87 s vs. 34.57 ± 16.25 s; mean difference 7.63 s, 95% CI 0.90–14.35; p = 0.027, g = 0.46), though accuracy was similar. COPE results showed a shift toward emotion-focused and avoidance strategies with increasing muscle pain. Conclusions: Bruxism is strongly linked to elevated anxiety, maladaptive coping strategies, and reduced inhibitory control speed. Integrating psychological screening tools such as GAD-7 and COPE into dental and medical practice could enable early identification and referral, supporting comprehensive management that combines dental treatment with psychological interventions to prevent chronic pain and long-term complications. Full article

Building psychological safety is vital for preventing workplace bullying and for sustaining employee well-being, organizational performance, and work engagement. This study developed and evaluated a short-term intervention promoting psychological safety and communication skills. The program provided strategies for addressing during high-stress situations and fostered a shared understanding of honest communication. A cluster randomized controlled trial was conducted in a pharmaceutical company, with departments assigned to intervention or control groups. The effects were examined using a generalized linear mixed-effect model. In the intervention group, psychological safety with supervisors and teams significantly increased, preceding a significant rise in consultation tendencies, whereas bullying and supervisor-related intimidation significantly decreased. Work engagement declined in the control group but remained stable in the intervention group. These findings demonstrate that the program enhanced psychological safety, enabling employees to express opinions without irrational fear, which in turn increased consultation, improved communication, reduced bullying, and sustained work engagement. The group-based format further emphasized mutual understanding and skill-sharing, providing broad organizational benefits. This study positions psychological safety as a proximal mechanism that facilitates consultation behaviors and demonstrates the feasibility of integrating brief, evidence-based training into routine organizational development. Full article

Aluminum (Al) toxicity is a major limiting factor for plant growth and development in acidic soils. Melatonin, a plant growth regulator and signaling molecule, enhances resistance to multiple stresses. Recent studies show that melatonin alleviates Al toxicity through several complementary mechanisms. Here, we first outline the physiological and molecular impacts of Al stress and the external and internal strategies plants use to cope with it. We then summarize melatonin biosynthesis and its broader roles in stress adaptation. We focus on recent advances in melatonin-mediated mitigation of Al toxicity, highlighting four principal mechanisms: (i) the activation of antioxidant defense systems, (ii) the stimulation of organic acid anion exudation that chelates Al in the rhizosphere, (iii) the modification of cell wall composition to reduce Al binding sites, and (iv) the promotion of intracellular Al sequestration. We also discuss the crosstalk between melatonin and nitric oxide, as well as interactions with phytohormone signaling. Collectively, this review comprehensively synthesizes the current understanding regarding the role of melatonin in alleviating Al toxicity in plants, offering a promising strategy for crop production in acidic environments. Full article

Rootstock choice offers a powerful lever for tailoring economically important trees to adverse environments. Camellia oleifera Abel., a premier oil-producing species cultivated widely on red-soil hills, suffers large yield losses under chronic phosphorus deficiency. We grafted a single elite scion (CL4) onto three contrasting rootstocks (CL4, CL3, CL53) and monitored growth and root transcriptomes for 1.5 years under adequate (1 mM) or limiting (0 mM) P supply. Under low-P stress, the rootstock identity reshaped the root architecture: CL4/CL3 produced the longest, most extensive network, increasing the total root length by 49.7%, the surface area by 52.9%, and the volume by 42.6% relative to the control, whereas leaf morphology responded solely to P supply, not to the graft combination. CL4/CL3 also accumulated up to more than 17.5% of root biomass and 28.25% of whole-plant biomass than any other combination. Physiologically, CL4/CL3 acted as an aggressive P miner, accumulating 67.8% more P in its roots than the self-grafted control under P limitation, while CL4/CL4 maximized the internal P use efficiency, showing a 44.74% higher root P use efficiency than CL4/CL53—two contrasting yet effective strategies for coping with low-P stress. Transcriptome profiling uncovered 1733 DEGs in the CL4/CL3 and 2585 in the CL4/CL4 roots, with 150 and 255 uniquely co-expressed genes, respectively. CL4/CL3 up-regulated organic-acid and phenylpropanoid pathways; CL4/CL4 activated defense and phosphate transport networks. qRT-PCR of six genes confirmed that CL4/CL3 mounted a stronger low-P response via MAPK, hormonal, and lipid–metabolic signaling. These results provide a mechanistic framework for rootstock-mediated P efficiency and establish a foundation for the molecular breeding of C. oleifera under nutrient-limited conditions. Full article

The widespread use of chemical fertilizers has raised concerns because of their environmental impacts, including soil degradation, water contamination, and biodiversity loss. The integration of organic amendments into agricultural systems provides a sustainable alternative. This study investigates the molecular and physiological traits underlying rapeseed responses to organic amendments based on poultry and plant material mixed with the soil. Plant growth, CO₂ assimilation, metabolic, proteomic, and soil microbial analyses were performed. Results show a significant stimulation of plant growth (100%) and leaf biomass (200%) following amendment application. This response is attributed to enhanced efficiency in light energy use for CO₂ fixation, increased carbohydrate and amino acid production, and improved biomass and yield. Increased upregulation of proteins and antioxidant metabolites such as abscisic acid (ABA) indicates an enhanced capacity to cope with oxidative stress. The amendments activated metabolic mechanisms that improved redox balance and homeostasis, including more efficient light energy use and enhanced antioxidant synthesis. Furthermore, the organic amendments promoted Actinobacteria in the soil, contributing to improved soil quality. These metabolic responses may enhance plant resilience against oxidative stress and environmental fluctuations. These findings highlight promising strategies to enhance crop productivity and resilience, advancing sustainable agriculture and strengthening future food security. Full article

The present study examined the relationship between sleep duration and eating behaviours, stress symptoms, and burnout in healthcare professionals. Objective: The present study aimed to examine whether sleep duration influenced the psychosocial well-being of healthcare personnel, as well as to identify possible predictors and patterns of vulnerability in this population. Method: A cross-sectional study was conducted with a sample of 194 public healthcare workers (mainly women and nursing staff). Validated questionnaires were used: the EAT-40, the EDI, the MBI, and a stress symptom scale. The participants were classified into two groups according to their sleep duration (fewer than six hours of sleep and six hours or more of sleep). Results: A sleep duration of fewer than six hours was associated with higher levels of depersonalisation (burnout), physical and emotional symptoms of stress (fatigue, tachycardia, memory loss, crying easily), dysfunctional coping strategies (self-medication, isolation), and more restrictive eating behaviours. A regression analysis identified seven predictors of sleep duration: allergies, marital status, hours worked, depersonalisation, alcohol consumption, interpersonal distrust, and skipping meals, which together explained 18% of the variance. A network analysis showed positive correlations between these variables in the group with a shorter sleep duration, indicating a pattern of cumulative psychosocial vulnerability. Conclusions: Although the cross-sectional design limits causal inference, the results underscore the importance of sleep as a key factor in the emotional and functional well-being of healthcare personnel. Organisational interventions focused on promoting rest, emotional management, and stress prevention are suggested, considering sleep not only as a biological need, but also as a relevant indicator of psychosocial health for healthcare quality. Full article

Background: Urban institutions offer quality post-secondary education to individuals who may not otherwise have access, yet do not always provide necessary resources for racially marginalized students. Students at primarily White, urban institutions face race-based stressors, such as microaggressions. According to stress and coping theory, individuals respond to stressors through coping strategies, which differ in effectiveness and subsequent outcomes. Social justice advocacy (SJA) represents a potentially important avenue for coping with discrimination, yet the relationship between microaggressions and SJA, as well as the association of task-focused coping with SJA, is unclear. Methods: A total of 96 students with racially marginalized identities were surveyed. Results: Analyses revealed significant associations between experiencing racial microaggressions, coping, and engagement in SJA, as well as a partial mediations of the associations between racial microaggressions and engagement in SJA through task-focused coping. Conclusions: The study offers support for the established association between discrimination and coping, including SJA. Results indicate that consideration should be given to SJA and task-focused coping as encouraged responses to racial microaggressions. Future studies should evaluate the outcome of these coping efforts and consider alternative ways of coping. Full article

Background and Objectives: The autonomic nervous system (ANS) orchestrates adaptation to stress; however, its reactivity is influenced by demographic, anthropometric, and psychosocial factors. While arterial stiffness and central adiposity are established cardiovascular risk markers, less is known about how maladaptive coping strategies, cumulative life stress, and quality of life influence short-term autonomic regulation. This study examined the age- and sex-specific associations between anthropometry, maladaptive coping, life stress, quality of life, and ANS adaptation in adults. Materials and Methods: In this cross-sectional study, 122 healthy adults aged 21–78 years underwent a standardized lay–stand–lay (LSL) protocol with pulse wave analysis. Hemodynamic outcomes included pulse wave velocity (PWVao), augmentation indices (AIxA and AIxB), and aortic blood pressures (SBPao and PPao). Anthropometric measures comprised BMI, waist and hip circumference, waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR). Psychosocial assessments included the Young Hypercompensation Inventory (maladaptive coping), Holmes–Rahe Life Events Inventory (life stress), and EQ-5D-3L (quality of life). Associations were analyzed using mixed-effects models adjusted for covariates, with false discovery rate correction. Results: Age was the strongest determinant of autonomic reactivity: older adults showed greater recovery of augmentation indices and central pressures after orthostatic challenge. Sex differences were evident, with women displaying consistently higher augmentation indices and men showing greater PWV responses. Central adiposity (WHR, WHtR, and waist circumference) predicted blunted augmentation index reactivity, while hip circumference was protective. BMI-defined obesity showed weaker associations. Maladaptive coping, life stress burden, and quality of life were not significantly associated with ANS indices after correction for multiple comparisons. Conclusions: ANS adaptation to postural stress is largely determined by age, sex, and visceral adiposity, whereas psychosocial measures showed limited influence in this healthy adult sample. These findings highlight the demographic and anthropometric determinants of cardiovascular adaptability, suggesting that psychosocial influences may primarily act through long-term behavioral and neuroendocrine pathways. Full article

Background: Informal caregivers of individuals with mild cognitive impairment (MCI) experience high levels of psychological and physical stress, with limited access to supportive interventions and time constraints. Virtual Reality (VR) technologies may provide brief and accessible opportunities to support caregiver well-being, particularly during waiting periods in clinical settings. This pilot study aimed to explore the potential of a semi-immersive VR intervention to enhance psychological well-being in informal caregivers. Methods: This non-randomized pilot study investigated the effects of a semi-immersive VR-based physical training program (K-HERO^®) on psychological well-being and coping strategies in informal caregivers. Participants were recruited from January to May 2025 at the IRCCS Centro Neurolesi “Bonino-Pulejo” (Messina, Italy), and the intervention was delivered individually in a dedicated room within the rehabilitation facility, while caregivers accompanied their relatives to treatment sessions. Ten caregivers completed six sessions (30–40 min each). The study was conducted in accordance with TREND reporting guidelines. Pre- and post-intervention assessments included the State-Trait Anxiety Inventory (STAI), Perceived Stress Scale (PSS), COPE Inventory, Caregiver Burden Inventory (CBI), and International Physical Activity Questionnaire (IPAQ). Visual Analog Scales and instrumental data from the VR system were used to monitor physical performance and user experience. Non-parametric statistics were applied. Results: Significant reductions were observed in avoidance (p = 0.033) and social support-based (p = 0.023) coping strategies. Differences emerged based on caregiver-patient relationships: parental caregivers showed increased anxiety, while offspring caregivers showed improvements. The intervention was well tolerated, with high usability and no adverse events reported. Conclusions: A short, structured, VR-based intervention delivered during clinical waiting periods may effectively reduce maladaptive coping strategies and support emotional well-being in informal caregivers. These findings highlight the potential of brief digital interventions in real-world care contexts. Larger randomized studies are needed to validate these preliminary findings and personalize interventions to different caregiver profiles. Full article

Background/Objectives. This study applied the Demands-Resources-and-Individual-Effects(DRIVE)-Nurses-Model to explore and compare the experiences of nurses working in Intensive Care Units (ICUs) and in Neonatal Intensive Care Units (NICUs), by investigating the effects of the interplay (main/mediating/moderating effects) of perceived stress related to dealing with death/critically ill patients (Death-and-Dying-Stressor)—which unavoidably features in the daily life of nurses working in ICU/NICU—with further potential Stressors in Nursing (Conflicts-with-Physicians, Peers, Supervisors, Patients/their families, Uncertainty-Concerning-Treatment, Inadequate-Emotional-Preparation, Discrimination, Workload), Work-Resources (Job-Control, Social-Support, Rewards), and Coping-Strategies (Problem-focused, Seek-Advice, Self-Blame, Wishful Thinking, Escape/Avoidance) on nurses’ psychological health conditions according to the working unit (ICU/NICU). Methods. Overall, 62 critical care nurses (ICU = 35; NICU = 27) completed self-report questionnaires. Main/mediating/moderating effects were tested by using Correlational-Analyses and Hayes-PROCESS-tool by working unit. Results. Nurses working in NICU reported higher Psychological Disease than nurses working in ICU. The detrimental psychological impact of Death-and-Dying-Stressor was mediated by Conflicts-with-Supervisors-Stressor among ICU nurses and by Uncertainty-Concerning-Treatment and Conflicts-with-Physicians stressors among NICU nurses. The recourse to Self-Blame and Escape/Avoidance coping strategies exacerbated the psychological risk among ICU nurses, while perceived Work-Resources (Job-Control/Social-Support) played a protective moderating role among NICU nurses. Conclusions. The application of the DRIVE-Nurses-Model to deepen the experience of nurses working in ICU/NICU could advance the understanding of the mechanisms underlying the relationship between Death-and-Dying-Stressor and nurses’ psychological health, suggesting tailored risk profiles and accounting for key protective factors, to provide nurses with the necessary resources for adjusting to their challenging and emotionally demanding work-related duties and experiences. Full article

Academic stress arises from students facing academic demands and is linked to various academic and psychological factors. However, research has yet to explore its potential correlations with somatization, overall health issues, studying strategies, academic procrastination, academic performance, and intelligence scores. The objective of this study was to determine the potential correlations between such variables with academic stress in a sample of health sciences university students. University students of different bachelor’s programs were invited to participate; they fulfilled an electronic questionnaire with personal and psychological variables, including academic stress, and performed an intelligence test, which measures verbal and non-verbal intelligence. Finally, their academic achievement was measured with the grade point average (GPA). A total of 437 students were included, of which 296 (67.7%) were women, with a mean age of 20.36 ± 2.61 years old. Academic stress was higher in women than in men and showed moderate positive correlations with anxiety, depression, and somatization and a low positive correlation with the sum of diseases. It also showed a low negative correlation with sleep quality. In addition, academic stress correlated negatively with self-motivation, emotion perception, and emotion management as well as with active coping, positive relations with others, and the studying strategies (self-regulation, effort regulation, critical thinking, and time and study environment). We also observed a low positive correlation between academic stress and academic procrastination, which was higher in women than in men. No correlations were found with GPA or intelligence scores. In conclusion, academic stress was positively correlated with somatization, depression, anxiety, the sum of diseases, and academic procrastination; it was negatively correlated with emotional intelligence (mainly self-motivation), active coping, and specific studying strategies. Full article