Search Results (208)

Mediterranean organic viticulture requires sustainable pest management strategies that leverage local soil biodiversity. This study isolated endemic entomopathogenic fungi from vineyard soils in Nemea, Greece, using a dual-insect baiting system with Tribolium confusum and Sitophilus spp. The recovered isolates caused complete mortality in bait insects, with mycelial emergence from 93.75% of cadavers. DNA sequencing of the ITS1 region identified the recovered isolates as Purpureocillium lilacinum. Phylogenetic analysis revealed that Nemea isolates (TD and TM series) form a monophyletic clade with 100% bootstrap support, showing distinct genetic divergence from the reference strain P. lilacinum NRRL 895—evidence of a unique “microbial terroir.” Virulence assays demonstrated species-dependent mortality against stored-product pests: Sitophilus granarius was the most susceptible (76.7% mortality; LT₅₀ = 1.9 days), followed by Sitophilus zeamais (61.1%; LT₅₀ = 2.7 days), Tribolium confusum (56.7%; LT₅₀ = 2.8 days), and Sitophilus oryzae (50.0%; LT₅₀ = 3.3 days). Mycosis confirmation (65–83%) and 0% control mortality confirmed pathogenicity. As locally adapted biological control agents, these endemic P. lilacinum strains are highly suitable for protecting crops from major insect pests. Full article

Background/Objectives: The bean beetle (Callosobruchus maculatus) exhibits population density-dependent plasticity in the terminal oocyte maturation rate. DNA methyltransferase 1 (DNMT1) plays a conserved function in reproduction that is independent of DNA methylation. However, whether DNMT1 is involved in the population density-dependent reproductive plasticity of bean beetles remains unclear. Methods: Two and twenty pairs of beetles were reared with approximately 100 seeds per bottle to establish a low-density population and a high-density population, respectively. Quantitative real-time PCR was used to unveil the mRNA levels of DNMT1, MBD2/3, and insulin-like peptides (ILPs). RNA interference was used to determine the function of DNMT1 and MBD2/3 in terminal oocyte development. The length of terminal oocytes was measured under a microscope. Results: Individuals reared under high-population-density conditions showed a faster terminal oocyte maturation rate compared to those under low-density conditions. The bean beetle genome encodes DNMT1 but lacks DNMT3, and only a single methyl-DNA-binding domain protein (MBD2/3) was identified. Population density could modulate the expression levels of both DNMT1 and MBD2/3. RNA interference (RNAi)-mediated knockdown demonstrated that suppressing either DNMT1 or MBD2/3 significantly reduced the terminal oocyte maturation rate. Moreover, silencing DNMT1 and MBD2/3 resulted in decreased expression of ILP3 and all ILPs in the fat body, respectively. ILPs are known to be involved in regulating terminal oocyte development. Conclusions: Taken together, these findings suggest that DNMT1 and MBD2/3 modulate the population density-dependent terminal oocyte maturation rate in the bean beetle by influencing the expression of ILPs. Full article

Effective monitoring of insect populations is essential for sustainable pest management and for supporting Integrated Pest Management (IPM) strategies that reduce reliance on chemical pesticides. Bioacoustic methods have recently emerged as a promising approach for monitoring insects by analyzing the sounds and vibrations they produce during activities such as feeding, movement, and communication. This review examines the application of bioacoustics in insect monitoring and pest management, with particular emphasis on recent advances in artificial intelligence (AI) and automated detection technologies. The biological foundations of insect sound production, acoustic monitoring technologies, and AI-based analytical methods are discussed. Machine learning and deep learning models enable automated detection and classification of insect species based on acoustic signals, facilitating early pest detection and biodiversity monitoring. Bioacoustics has been applied to detect and identify insect pests, monitor stored-product insects, and manipulate insect behavior using acoustic and vibrational signals. Despite these advances, challenges remain, including environmental noise interference, limited acoustic datasets, and technical constraints of monitoring systems. Future research should focus on improving datasets, signal processing methods, and the integration of bioacoustics monitoring with precision agriculture and IPM frameworks to support sustainable crop protection. Full article

Storage insects consist a continuous global threat to food safety. In this study, the efficacy of the entomopathogenic nematodes (EPNs) Steinernema khuongi and Heterorhabditis downesi was evaluated against larvae of Tribolium castaneum, Tribolium confusum, Tenebrio molitor, and Trogoderma granarium in wheat and maize at six concentrations and two exposure intervals. Both EPNs exhibited higher virulence when applied in wheat than in maize. Generally, larval mortalities were higher under H. downesi treatments vs. S. khuongi, both in commodities and exposures. Notably, the tested EPNs caused high mortalities to T. granarium larvae (range, 88.9–92.2%) and T. castaneum larvae (range, 81.1–94.4%), respectively, at 10,000 IJs/mL in wheat vs. mortality ranges 72.2–77.8% and 74.4–87.8% in maize, respectively. In contrast, T. confusum larvae were tolerant to both EPNs. Tenebrio molitor larvae were tolerant to S. khuongi (<34.0% mortality) whereas susceptible to H. downesi (>83.3% mortality) at 10,000 IJs/mL in wheat and maize. These findings highlight the potential of S. khuongi and H. downesi as beneficial organisms against several stored-product insect pests. Full article

The cigarette beetle, Lasioderma serricorne, is a globally important pest of stored products, and prolonged fumigant use has accelerated resistance development. Glutathione S-transferases (GSTs) are key phase II detoxification enzymes that mediate insect tolerance to xenobiotics. In this study, we identified nine GST genes (LsGSTs) in L. serricorne and classified them into four cytosolic classes, namely epsilon, delta, theta, and sigma, based on phylogenetic analysis. Most LsGSTs were predominantly expressed during larval stages, while LsGSTs7 showed peak expression in adults. Tissue-specific profiling revealed predominant expression in metabolically active organs, including the fat body, Malpighian tubules, and midgut. Inhibition of GST activity using diethyl maleate (DEM) significantly increased larval susceptibility to three emerging fumigants: ethyl formate, benzothiazole, and methyl isothiocyanate. Exposure to LC₃₀ and LC₅₀ concentrations of these fumigants induced up-regulation of multiple LsGSTs, highlighting fumigant-specific detoxification responses. RNA interference targeting nine fumigant-inducible LsGSTs markedly elevated mortality and decreased total GST activity under fumigant stress. Furthermore, recombinant LsGSTs6 protein effectively metabolized methyl isothiocyanate, confirming their direct role in fumigant detoxification. Collectively, these findings provide novel insights into the molecular mechanisms underlying GST-mediated tolerance in L. serricorne and identify specific GST isoenzymes as promising molecular targets for innovative resistance management strategies in stored-product pest control. Full article

Acoustic detection methods offer a non-destructive alternative to manual inspection for identifying insect infestations in stored products, but their performance is compromised by ambient noise in operational environments. This study presents an enhanced detection algorithm for the Acoustic Stored Product Insect Detection System (A-SPIDS) that enables reliable single-insect detection in the presence of strong external noise. The platform’s physical noise isolation achieved an average attenuation of 45 $\mathrm{d}$$\mathrm{B}$ above 2000 $\mathrm{Hz}$. Spectral analysis revealed that insect signals dominate over ambient noise, generating insect-like impulses in the high-frequency band, enabling optimization of the Normalized Signal Pulse Amplitude (NSPA) detection metric to the 1565 $\mathrm{Hz}$ to 6000 $\mathrm{Hz}$ frequency band, resulting in 99.4% detection accuracy at 80 $\mathrm{d}$$\mathrm{B}$A ambient noise levels. The external microphone was leveraged to identify and remove noise-generated impulses from internal piezoelectric sensor recordings, achieving 100% detection with zero false alarms across the recorded dataset featuring species Callosobruchus maculatus, Tribolium confusum, and Tenebrio molitor in oatmeal, rice, wheat, and corn products at noise levels exceeding 100 $\mathrm{d}$$\mathrm{B}$A. Full article

This study aimed to biosynthesize silver nanoparticles (AgNPs) using aqueous leaf extract of Galega officinalis and to evaluate their insecticidal activity against key stored-product pests. AgNP formation was confirmed through UV–vis spectroscopy, which showed a surface plasmon resonance peak at 380 nm. FTIR analysis indicated the presence of plant-derived functional groups likely involved in the reduction and stabilization of Ag⁺ ions. Dynamic light scattering revealed an average hydrodynamic diameter of 25.07 nm, a PDI of 0.39, and a zeta potential of −22 mV, while TEM images showed predominantly spherical and polydisperse particles ranging from 4.3 to 42.4 nm. Insecticidal bioassays performed on Sitophilus granarius, Tribolium confusum, Plodia interpunctella, and Ephestia kuehniella revealed concentration-dependent mortality. The highest mortality rates were recorded at 1000 ppm, reaching 100% in T. confusum, 83.33% in P. interpunctella, and 76.67% in both S. granarius and E. kuehniella. These findings demonstrate the potent insecticidal activity of G. officinalis-mediated AgNPs and support their potential as environmentally friendly alternatives for stored-product pest management, warranting further studies on safety, large-scale synthesis, and integration into pest-control programs. Full article

Microwave heating has been widely used for disinfestation in the food industry due to its selective heating. However, research on the effects of microwave heating on stored product insects is still relatively limited, which has restricted its broader application in grain pest control storage. Therefore, this study evaluated the lethal effects of different microwave powers and exposure times on three major pests in paddy and investigated the impact of microwave treatment on improving adult detection efficiency, intending to develop a rapid and efficient detection method for stored grain insects. The results showed that the mortality of Sitophilus oryzae, Tribolium castaneum, and Oryzaephilus surinamensis increased with the increase in microwave power and exposure time. Specifically, 100% mortality was achieved for both S. oryzae and T. castaneum at 700 W for 60 s exposure. However, higher power levels and longer exposure durations exacerbated the non-uniformity of grain temperature distribution and adversely affected the germination rate. In addition, microwave treatment at 350 W, 490 W, and 700 W significantly reduced fungal load in paddy. The moisture content and water activity of rice decreased with the increase in microwave power and exposure time, while the percentage of grain breakage remained largely unaffected. These findings indicated that microwave treatment can effectively control insects and fungi without significantly altering the main physical properties of paddy. Notably, microwave treatment with short exposure durations (20–30 s) at all three power levels is conducive to increasing the recovery percentage of S. oryzae adults, while microwave treatment at low power (350 W) with exposure durations of 25–40 s helps improve that of T. castaneum. Accordingly, microwave heating is not only a promising strategy for protecting stored grains but also has potential for development as a rapid detection method for specific insect pests. Full article

Mating disruption (MD) is an environmentally friendly pest management approach that uses synthetic pheromones to interfere with insect mate location and reproduction. This review summarizes current progress in the application of MD for stored-product pests, with emphasis on Lepidoptera (Plodia interpunctella Hübner and Ephestia kuehniella Zeller (Pyralidae)) and Coleoptera (Sitophilus spp. (Curculionidae)). For moth pests, numerous studies have demonstrated substantial suppression of mating and population growth under both laboratory and field conditions, particularly when MD is integrated with sanitation, monitoring and other IPM measures. Conversely, MD applications against beetles have been less successful due to their aggregation-based communication and lower volatility of their pheromones. Advances in pheromone formulation technology, including polymer dispensers, microencapsulated sprays and aerosol emitters, have improved pheromone stability and controlled release, although achieving uniform coverage in large and aerated storage environments remains challenging. The integration of MD with biological control, temperature management and reduced fumigant use offers promising directions for sustainable pest suppression. Continued development of smart-release devices, long-term field validation and integration with automated monitoring systems will further enhance the feasibility and cost-effectiveness of MD. Overall, MD represents a key behavioral component in reducing pesticide reliance and promoting sustainable management of stored-product pests. Full article

The pomegranate (Punica granatum L.) fruit peel is an agro-industrial by-product rich in bioactive compounds. In this study, the bioactivity of pomegranate peels (cv. Ako) extracted with acetone, diethyl ether, and n-hexane was assessed by evaluating toxic (contact and ingestion), repellent, antifeedant, and nutritional effects towards Sitophilus granarius (L.) (Coleoptera, Curculionidae) adults. Contact toxicity assays revealed significant mortality induced by the acetone and n-hexane extracts, with 24-h LD₅₀ values of 76.93 and 81.14 $\mathsf{\mu }$g/adult, respectively. In ingestion bioassays, at the highest dose tested (750 $\mathsf{\mu }$g/disk), the acetone pomegranate peel extract showed a strong feeding deterrence (FDI: 80%), and significantly reduced food consumption (RCR) and relative growth rate (RGR). In filter paper repellency assays, the acetone extract induced positive contact repellency, with PR values ranging from 80% to 30%. GC-MS analysis identified sitosterol, 9,12-octadecadienoic acid, and $\alpha$-tocopherol as the major constituents of the acetone extract. These results highlight the potential of pomegranate peel as a sustainable source of bioactive compounds for stored-product insect pest management. Full article

The present study aimed to document the diversity and seasonal dynamics of stored-product insects in an animal feed facility located in northern Greece. A total of 38 traps were installed across different operational areas of the facility and inspected over 51 consecutive sampling occasions. Captured insects were identified to the lowest possible taxonomic level, and their frequency and dominance were calculated. In total, 9047 insect species belonging to five orders, 14 families, and at least 18 insect species were recorded. The dominant species were Tribolium castaneum, T. confusum, Oryzaephilus surinamensis, Sitophilus granarius, Lasioderma serricorne, and Lepidoptera adults, which collectively accounted for more than 85% of all captures. The total number of insects exhibited marked seasonal fluctuations, with the highest captures during late summer and early autumn and minimal activity during winter. Positive and significant correlations were detected among several dominant species, notably between Lepidoptera and T. castaneum, suggesting overlapping environmental preferences within the facility. These findings provide a detailed overview of the insects associated with feed industries in Greece and underscore the importance of continuous monitoring for effective pest management. The results highlight the need for seasonally adjusted control measures and contribute to a better understanding of the dynamics of stored-product pests under industrial conditions. Full article

Botanical insecticides have gained interest due to a rising demand for environmentally friendly pest control methods for stored-product protection. The insecticidal effectiveness of the essential oil (EO) obtained from the oleo-gum-resin of myrrh (Commiphora myrrha (Nees) Engl.), against Prostephanus truncatus (Horn) and Sitophilus zeamais Motschulsky, and the metabolic shifts of the two species, were investigated in this work. A thorough gas chromatography-mass spectrometry (GC-MS) investigation showed that the composition of this EO was dominated by furanosesquiterpenes, specifically, furanoeudesma-1,3-diene and curzerene. Commiphora myrrha EO treatments, especially at 1000 ppm, resulted in high adult mortality for P. truncatus (up to 85.6%), while S. zeamais showed only moderate mortality (up to 25.6%). To investigate the different species-specific effectiveness of the EO, untargeted GC-MS metabolomic profiling was conducted to elucidate the impact of the EO on the metabolism of the insects, with subsequent data analysis employing multivariate, univariate, and network methods. Each species reacts differently to the treatments (myrrh EO versus the synthetic insecticide pirimiphos-methyl (PM)), according to the analysis results. In particular, myrrh EO caused distinct shifts in metabolic pathways that varied between P. truncatus and S. zeamais. Overall, C. myrrha EO exhibits potential as a botanical insecticide, especially against P. truncatus, and it causes metabolic disturbances specific to the species. The results demonstrate the significance of metabolomic technologies in assessing bioinsecticide mechanisms and lend credence to their possible incorporation in integrated pest management methodologies or their contribution to the creation of diagnostic indicators of insecticidal exposure. Full article

In recent years, interest in edible insects has increased in Western countries, leading to an expansion of the market for insect-based products. In this context, it is essential to assess their susceptibility to infestation by stored-product pests, to ensure food safety and to develop appropriate storage management strategies. This study examined the ability of three common stored-product moth species (Plodia interpunctella, Corcyra cephalonica and Ephestia kuehniella) to infest Acheta domesticus powder and biscuits enriched with cricket powder. Larval development, adult emergence, wingspan and female fertility were evaluated. The results showed that P. interpunctella and C. cephalonica were able to complete their development on cricket powder, albeit with lower survival rates, longer developmental times and fewer offspring than on the standard diet. E. kuehniella was unable to develop on cricket powder and only minimal adult emergence was recorded in the biscuit trials, although signs of infestation were detected. These findings demonstrate that stored-product moths represent a potential infestation risk for this novel food, the market for which is expected to grow. Full article

The genus Sclerodermus Latreille (Hymenoptera: Bethylidae) comprises over 80 species of ectoparasitoids of insect pests in forests, agricultural environments, and stored products with a cosmopolitan distribution. Despite its growing significance in biological control, behavioral ecology, and public health, the taxonomy of the genus remains poorly resolved. This is largely due to morphological reduction and simplification among species, outdated or incomplete original descriptions, and limited access to type material. A particularly problematic case is Sclerodermus cereicollis Kieffer, originally described from two geographically disjunct populations: Giglio Island (Italy, Palaearctic) and Annobón Island (Equatorial Guinea, Afrotropical). The syntype series includes morphologically divergent specimens, casting doubt on their conspecificity. In this study, we redescribe S. cereicollis based on both the original syntypes and newly collected material from Italy. A lectotype is designated to stabilize the nomenclature, and we provide the first molecular data for the species to assess genetic cohesion among populations. Comparative morphological and molecular analyses reveal that the Afrotropical syntypes represent a distinct, previously undescribed species. Accordingly, we describe Sclerodermus annobonensis Masini, Colombo & Azevedo sp. nov., designating a holotype. This study refines species boundaries within Sclerodermus and highlights the value of integrative taxonomy, combining historical and contemporary data, in resolving persistent systematic ambiguities in morphologically conservative taxa. Full article

The present study highlights the critical role of surface type, insect species, and exposure duration in determining the efficacy of surface-applied insecticides in stored-product pest management. Four insecticides were sprayed and evaluated on different surfaces (concrete, metallic, plastic, and ceramic) against two beetles: the red flour beetle and the tobacco beetle. Alpha-cypermethrin and spinosad exhibited rapid and high efficacy, particularly on non-porous surfaces such as metal and ceramic, whereas pirimiphos-methyl was less effective initially and required extended exposure to achieve complete mortality, especially against Tribolium castaneum. In contrast, Lasioderma serricorne showed greater susceptibility across all insecticides and surfaces. Spinosad maintained high efficacy across all surface types, suggesting broader applicability under variable conditions. The reduced performance of insecticides on concrete surfaces underscores the influence of substrate porosity on insecticide bioavailability. Additionally, the observed delayed mortality effect in all treatments indicates that even brief exposure can result in lethal outcomes, emphasizing the long-term potential of these applications. These findings underscore the need for surface-specific application strategies and support the integration of surface treatments into comprehensive pest management programs. Further research is warranted under simulated field conditions to assess residual efficacy over time and in the presence of food, thereby enhancing the relevance of laboratory findings to real-world storage environments. Full article