Search Results (109)

Spontaneous imbibition (SI) is the key mechanism governing oil production in fractured carbonates, where capillary forces drive oil from the low-permeability matrix into the fractures. Its application is limited by the strongly oil-wet nature of carbonate rocks and by high interfacial tension (IFT), which restrict the capillary forces needed to mobilize oil from the matrix. This work examines whether a combined low-salinity water (LSW)-surfactant enhanced oil recovery (EOR) process can be optimized for SI by tuning the potential determining ions (PDIs) of the hybrid-stage brine. Experiments were performed with a fixed LSW preconditioning stage, while the hybrid-stage composition and surfactant concentration were varied. Contact angle screening of three ion-modified LSW formulations showed that LSW3Ca3Mg-SO₄, with tripled Ca²⁺ and Mg²⁺, reduced the contact angle from 178.5° to 47.3° within 6 days and lowered the IFT from 33.2 to 17.5 mN/m without surfactant. In SI tests on limestone cores, ion-modified hybrid systems showed substantially higher early-time imbibition rates than conventional LSW-surfactant systems, together with equal or higher ultimate recovery. Notably, with 0.5 wt.% surfactant the ion-modified system reached an early-time rate of 0.75%/h and 36.36% of the original oil in place (OOIP), compared with 0.36%/h and 35.94% OOIP for conventional LSW with 1.0 wt.% surfactant, i.e., at half the surfactant dosage. Wettability-controlled systems, such as ion-modified LSW without surfactant, accelerate early imbibition but recover only about 6–7% OOIP regardless of ionic tuning. IFT reduction, in contrast, is considered to lower the capillary resistance in tight pore throats, allowing much higher recovery even while the rock surface remains oil-wet (40.15% OOIP with the ion-modified hybrid system at 1.0 wt.% surfactant). These findings suggest that tuning the ionic composition of the hybrid-stage brine can accelerate SI and allow a lower surfactant dosage, pointing to a potentially cost-effective ionic design strategy for EOR in fractured carbonate reservoirs. Full article

Successful pregnancy depends on precise placental development, where trophoblast differentiation, syncytialization, invasion, and adaptation to metabolic stress are critical. Autophagy, a lysosome-mediated degradation pathway, has emerged as an important regulator of cellular homeostasis, yet its integrated role in trophoblast fate and functions has not been comprehensively summarised. This review synthesises current evidence on autophagy’s functions throughout placentation, from trophoblast differentiation to syncytialization and extravillous trophoblast invasion. We examine how autophagy enables cellular remodelling during differentiation, supports metabolic adaptation under hypoxia and nutrient stress, and maintains mitochondrial quality control through selective mitophagy. Autophagy is essential for syncytiotrophoblast formation via endoplasmic reticulum stress-coordinated activation and p53 downregulation. However, its effects on trophoblast invasion are context-dependent, influenced by oxygen tension, autophagic flux completeness, and differentiation state, which can potentially be shaped by parent-offspring genetic conflicts through genomic imprinting. Both excessive and insufficient autophagy contribute to pregnancy complications, including pre-eclampsia, foetal growth restriction, gestational diabetes mellitus, preterm birth, recurrent spontaneous abortion and obstetric antiphospholipid syndrome through distinct molecular mechanisms. Autophagy functions as a dynamically tuned homeostatic mechanism in placental development. Understanding condition-specific autophagy dysregulation is thereby crucial for improving pregnancy outcomes. Full article

Deep learning surrogate models trained with mean-squared-error loss produce statistically accurate but physically unconstrained flood predictions: water may flow uphill, appear spontaneously, or smooth over street-level corridors. In this work, a physics-informed training framework is developed for CNN-LSTM models that predict urban flood depths at 15 min intervals over a $128\times 128$ spatial grid. Three differentiable penalty terms are embedded directly into the loss function: (i) a gravity loss that penalizes depth increases against the water-surface-elevation gradient, (ii) a continuity loss enforcing local mass conservation with rainfall-adaptive thresholds, and (iii) a topography-aware false-alarm penalty modulated by the topographic wetness index (TWI). The framework is evaluated on the Norfolk, Virginia, flood dataset spanning two major storm events (August 2017 and September 2022) comprising 300 samples, with all variants trained on identical splits and robustness assessed over repeated random splits and leave-one-storm-out tests. A road-proximal evaluation restricted to a TWI-derived street mask quantifies street-level skill. The physics-constrained model achieves near-zero gravity violations (∼10⁻⁶) and the highest street-channel recall (0.77 ± 0.09 versus 0.44 ± 0.10 for the unconstrained baseline), the capability most relevant to downstream traffic routing, and its recall advantage more than doubles on a held-out storm, while a uniform false-alarm variant attains 16% lower mean absolute error but suppresses street recall to 0.25. The proposed TWI-modulated penalty reconciles this trade-off: it improves upon the uniform variant on every metric measured, recovering 60% higher street recall at the lowest MAE among all constrained variants and the best street-level F1 score. These results expose a fundamental tension between aggregate pixel-level error metrics and application-specific physical plausibility, and demonstrate that terrain-aware loss modulation offers a principled resolution. Full article

Rapid, low-cost ethanol quantification is vital for beverage quality control, biofuel production, and pharmaceutical applications, yet current approaches are costly, reagent- or label-dependent, or rely on spectroscopy with substantial sample preparation. We introduce a purely cell-based, label-free biosensor that exploits temperature-gradient-induced spontaneous detachment of Saccharomyces cerevisiae from a chip surface. The readout is the detachment half-time, t_d50, derived from time-resolved changes in interfacial thermal resistance, R_th, at the solid–liquid interface. Cells were pre-exposed to ethanol (0–70% v/v) and the detachment kinetics monitored using the heat transfer method (HTM). Under these conditions, cells display a pronounced non-monotonic t_d50 response with a peak around 20% v/v ethanol. Overall, the t_d50 rises from ~45 min (0% ethanol) to ≳10 h (20%) and then decreases, with no detachment at 60% and beyond. Critically, cell quality gates the detachment window. Fresh yeast responds up to ~50%, whereas aged yeast ceases to detach above ~8%, demonstrating a dual-function assay. Complementary measurements show that ethanol decreases surface tension monotonically, as expected, while optical/SEM imaging reveals aggregation above the detachment window. Requiring only a heater and a temperature probe, this platform offers a compact and low-cost strategy for ethanol sensing. Its applicability in a complex matrix is further demonstrated using whiskey diluted to selected alcohol concentrations, which produced responses consistent with the ethanol calibration trend. Potentially, it also offers a thermal assay for real-time monitoring of microbial cell quality across biotechnology and bioengineering applications. Considering ethanol as a proxy for drugs, the strategy may also support label-free drug screening on cells. At a fundamental level, the non-monotonic effect of ethanol, and especially the sharp maximum at 20%, remains unresolved and invites further studies. Full article

During hydraulic fracturing, the extensive use of slickwater and post-fracturing shut-in (soaking) processes take advantage of spontaneous imbibition to displace crude oil. While nano-flooding agents are known to reduce interfacial tension (IFT) and alter wettability, a critical challenge lies in distinguishing between deep but inefficient displacement and shallow but highly efficient sweep. This study investigates the pore-scale mobilization and penetration depth of a nano-flooding agent in tight conglomerate reservoirs and focuses on the recovery per unit imbibition depth as a novel metric for evaluating the displacement efficiency. The nano-agent demonstrated excellent performance, reducing oil–water IFT to 0.141 mN/m and reversing wettability from oil-wet (148.7°) to water-wet (39.5°). Experiments revealed that the diffusion rate of the nano-agent decreases with pore size, suggesting a limited transport in confined space. Under reservoir conditions (80 °C), spontaneous imbibition in tight cores was highly permeability-dependent. High-permeability cores achieved a recovery rate of up to 44.6%, whereas low-permeability cores reached only about 12%. This work highlights that penetration depth alone does not necessarily indicate high recovery. The medium-permeability core exhibited a lower final penetration depth than the low-permeability core but achieved a much higher total recovery due to superior efficiency per unit depth, suggesting that in tight reservoirs, a shallow but highly efficient displacement mechanism can outperform a deep but inefficient one. Full article

Spontaneous imbibition modified by surfactants is a key technology for enhancing shale oil recovery. Currently, relevant studies mainly concentrate on marine shale worldwide, while the pore–fluid coupling characteristics of widely distributed medium-TOC terrestrial shale remain poorly understood. Against this background, this paper takes typical Paleogene terrestrial shale as the research object and integrates N₂/CO₂ adsorption and NMR T₂ spectroscopy to jointly characterize multiscale pore structures and dynamic fluid evolution during imbibition. The results show that the shale is dominated by mesopores in terms of pore volume, while micropores provide most of the specific surface area. The zwitterionic surfactant HPSB can greatly reduce oil–water interfacial tension and alter rock wettability, thereby breaking the high capillary resistance of micropores. During imbibition, water invades macropores first, followed by mesopores and micropores, and the entire process exhibits remarkable nonlinear dynamics controlled by multiscale pores. The 0.15% HPSB solution shows the best effect on activating micropores. This study innovatively quantifies the influence of surfactant concentration on fluid migration across different pore scales and reveals the internal mechanism of staged imbibition and micropore lag activation in terrestrial shale. It not only complements the global research system of shale imbibition theory but also offers practical guidance for the optimization of fracturing fluid systems in mesopore-dominated shale oil reservoirs. Full article

Conventional liquid springs enable storage of energy in the form of interfacial tension at forcibly wetted lyophobic surfaces. The pressure–volume work performed to compress the liquid into a poorly wettable porous medium is recovered during spontaneous expulsion when pressure falls below the capillary pressure characteristic of a given system. Our study explores generalizations to easily wettable materials where liquid infiltration is opposed solely by steric hindrance exerted on liquid molecules in micro-sized pores. The concept is exemplified in molecular simulations of prototypical model systems with methanol intruding narrow slits between hydrocarbon or graphene surfaces. While these materials show significant wetting propensities at macroscopic interfaces with liquid methanol, substantial compression is required to wet molecular-sized pores barely accommodating a monolayer of liquid molecules. The observed O(10³) bar intrusion pressures secure stored energy densities competitive with supercapacitors and amenable to improvement. Wall–liquid attraction and small pore diameters lead to intrusion–expulsion pathways along cooperative-adsorption isotherms. The process avoids abrupt liquid/vapor transitions and associated nucleation barriers, responsible for cycle hysteresis in experiments with water in hydrophobic capillaries. Using open ensemble (Grand Canonical) Monte Carlo sampling, we identify the range of porosities supporting reversible energy storage/recovery operation in lyophilic media; the results can assist with the design of molecular spring devices with competitive storage and power capacities in pragmatic contexts. Full article

Many of life’s biggest dilemmas can be summed up as a tension between holding on and letting go. The very language evokes a notion of intentionality which, for the most part, has evaded scientific understanding. How might we even get a window into it? Important insights have come from a seemingly simple task: wiggling one’s fingers to and fro to the beat of a metronome. As the metronome pace increases to some critical frequency, one coordinative pattern becomes unstable and switches spontaneously to another. Such transitions are typically preceded by critical fluctuations, a predicted feature of self-organization in complex, dynamical systems. Here we address the nature and source of these fluctuations, usually assumed to be: (1) random; (2) of external origin; and (3) of fixed magnitude. We performed an experiment in which participants were instructed to oscillate their fingers in either an in-phase or anti-phase pattern in time with a metronome and instructed them to either “hold-on” or “let-go” should they feel the pattern begin to change, yielding a 2 by 2 within-subjects design. We observed that as the metronome frequency was increased from 1.00 to 3.00 Hz, fluctuations in the relative phase between the fingers were significantly altered both by the starting coordinative pattern as well as the participant’s intention to “hold it on” or “let it go”. Specifically, the intention to hold on to the anti-phase pattern delayed the spontaneous transition to in-phase, an effect that was paired with increased fluctuations beyond the critical frequency. These observations were analyzed under the extended Haken–Kelso–Bunz (HKB) model which describes the non-linear stochastic dynamics of the order parameter (relative phase) as a gradient descent on a certain potential. Our analysis, in line with experimental results, suggests that intention transforms the HKB potential not only by stabilizing unstable coordination states but also (paradoxically) by increasing fluctuations around them. Such findings may offer new interpretative light on the relation between intention and fluctuations in the coordination dynamics of living things. Full article

The water huff-n-puff imbibition oil recovery technique has been recognized as an important approach to supplementing formation energy and recovering the remaining oil, attracting increasing attention. To further improve imbibition efficiency, a surfactant-aided huff-n-puff imbibition technique under high pressure was proposed. However, the imbibition mechanisms under high pressure, particularly under variable pressurization modes, remain insufficiently understood. In this study, the effects of different pressurization methods (constant vs. variable pressure) and surfactant types on imbibition behavior were systematically investigated. The results show that, compared with spontaneous imbibition, high-pressure imbibition increases oil recovery by 7–10% and the imbibition rate by 1–2 times, with the variable pressurization mode demonstrating a more pronounced enhancement. Surfactant selection should not pursue ultra-low interfacial tension (IFT) alone; instead, the wettability alteration ability is more critical. An optimal IFT–wettability synergy window is identified, through which the best imbibition performance is achieved when the IFT ranges from 10⁻² to 10⁻¹ mN/m and the contact angle ranges from 30° to 60°. Furthermore, the slug injection mode provides a synergistic effect with high-pressure variable pressurization and surfactant action. Compared with high-pressure formation water imbibition, surfactant-aided imbibition increases oil recovery by 10.44% and the imbibition rate by three times. These findings provide a deeper understanding of the key factors governing imbibition behavior and support the application of surfactant-aided huff-n-puff imbibition under high pressure in tight sandstone reservoirs. Full article

In response to the significant challenges posed by ice accumulation and contamination from various fluids in complex operating conditions for metallic materials, this study utilises picosecond laser precision machining to develop a ‘slippery surface’ featuring a micro-channel network structure. The core innovation of this study lies in the use of laser-machined micrometre-scale array textures to overcome the limitations of traditional isolated pores. These globally interconnected micro-channels serve as highly efficient reservoirs and dynamic transport channels for lubricants, significantly enhancing the interfacial capillary locking force of the lubricant. Experimental results demonstrate that this unique network geometry endows the surface with exceptional fluid replenishment and self-healing properties, enabling it to exhibit outstanding broad-spectrum hydrophobicity towards various fluids—including water, crude oil and ethanol (surface tension range: 17.9–72.0 mN m⁻¹)—with sliding angles consistently below 12°, whilst effectively slowing the dehydration and solidification processes of biological fluids. At a low temperature of −15 °C, the surface achieved an ice formation delay of up to 286 s, with an ice adhesion strength of only 33.9 kPa, ensuring that accumulated ice could be spontaneously detached under minimal external force. Furthermore, the micro-channel network structure serves as a key protective mechanism against mechanical wear, maintaining robust slippery properties even after three hours of high-pressure water jet scouring (Weber number of 300). This reliable interface, achieved through structural management, provides an efficient and scalable platform for addressing the all-weather anti-icing and antifouling requirements of outdoor infrastructure. Full article

The design and fabrication of metal matrix materials (MMCs), as well as the densification and joining of ceramic matrix composites (CMC), are still very challenging. For SiC- and C-based composites, liquid-assisted processing routes, such as the spontaneous infiltration process, emerge among the most cost-effective processes. To succeed in Ir-Si/SiC refractory composite fabrication by spontaneous infiltration, the wetting characteristics of the Ir-Si/SiC system, the surface and transport properties (surface tension and viscosity) of liquid Ir-Si alloys, and microstructural evolution at the interfaces formed between solid SiC (or C) with Ir-Si melt, have been carefully examined. Specifically, the wettability and interaction phenomena occurring at the Si-Ir eutectics/SiC interface as a function of temperature were investigated in the temperature range of T = 1350–1400 °C by the sessile drop method under an inert atmosphere with reduced oxygen content, and the results are presented and discussed in this paper. Taking into account the thermodynamics of the Si-C-Ir system, the interfacial phenomena and subsequent microstructural evolution are well-related to the process parameters, and the properties and characteristics of the as-produced interfaces may be predicted accordingly. The experimental conditions and results of wetting experiments, together with thermodynamic-based models predicting thermophysical property values of liquid Ir-Si alloys, are valuable key input data that are now available for the numerical study of infiltration processes. Full article

Tight conglomerate reservoirs exhibit strong pore-scale heterogeneity and extremely low permeability, in which spontaneous imbibition is primarily governed by capillary and viscoelastic effects. In this study, the imbibition dynamics of four representative fracturing fluid systems, including slickwater, 3% potassium chloride (KCl) brine, hydrolyzed polyacrylamide (HPAM) viscoelastic fluid, and a nanoemulsion (NE), were investigated using a temperature-controlled nuclear magnetic resonance (NMR) monitoring system. This approach enables real-time quantification of fluid uptake and pore-scale redistribution through time-resolved T₂ spectral analysis. The experimental results reveal a three-stage imbibition process consisting of rapid capillary-driven uptake, viscoelastic-retarded transition, and final equilibrium. Among the four fracturing fluid systems, the nanoemulsion exhibits the lowest interfacial tension (1.72 mN/m), the strongest wettability alteration, and the highest equilibrium recovery (0.76), which is nearly 80% greater than that of slickwater. Based on these observations, a multiscale capillary–viscoelastic coupling model was developed by extending the Lucas–Washburn framework to incorporate pore-size distribution, time-dependent wettability evolution, and viscoelastic damping. The model fits the experimental data well (R² > 0.90) and identifies viscosity as the most influential parameter controlling the imbibition rate (sensitivity = 0.78). Energy analysis further indicates that capillary energy dominates the early stage, whereas viscoelastic energy storage sustains fluid transport during the later stage. SEM observations were further used to qualitatively corroborate pore heterogeneity and pore–mineral associations, supporting the NMR-based pore-scale interpretation. This study provides a quantitative framework for describing non-Newtonian capillary flow in tight conglomerate rocks and enhances the understanding of capillary–viscoelastic interactions relevant to multiphase fluid migration. Full article

Background: Laryngomalacia is the most common cause of inspiratory stridor in infancy. While most mild cases resolve spontaneously, severe cases may require surgical intervention. We report a case of severe laryngomalacia successfully treated with correction of glosso-larynx (CGL), a surgical procedure originally developed for ankyloglossia with deviation of the epiglottis and larynx (ADEL). Methods: A 2-month-old infant with severe symptoms was evaluated using objective anatomical and functional metrics. The patient underwent CGL under local anesthesia to release restrictive tension on the hyoid-larynx complex. Results: The patient showed rapid and sustained improvement in respiratory symptoms. At the one-month follow-up, endoscopic examination confirmed functional airway expansion with limited to no morbidity. Conclusions: This case demonstrates that CGL may represent a feasible and minimally invasive therapeutic option for selected cases of severe laryngomalacia, particularly those involving complex tongue–larynx dynamics. Full article

Peripheral nerve injuries, especially neurotmesis, require precise repair strategies due to their severity and limited capacity for spontaneous regeneration. Nerve guidance conduits (NGCs) offer a promising alternative to autografts; however, consistent surgical techniques and anatomical references in rodent models could be enhanced. This ex vivo study focuses on describing and establishing a standardized, reproducible anatomical and technical protocol for implanting an NGC in the sciatic nerve of Wistar rats, identifying a 7 mm segment free of collateral branches as a safe site for neurotmesis. Thirty cadaveric hind limbs were positioned in lateral decubitus, and anatomical landmarks such as the greater trochanter, ischial bone, and femoral condyle guided the incision. A 1 cm scaffold was inserted and secured with 8-0 absorbable sutures, while muscle and skin were closed with 5-0 and non-absorbable sutures. The technique enabled safe access to the nerve, minimized risk to adjacent structures, and ensured proper scaffold positioning without tension. This standardized approach improves surgical reproducibility and supports anatomical integrity; however, because the study used ex vivo cadaveric samples, its capacity to facilitate functional nerve regeneration remains theoretical. While the protocol emphasizes the importance of surgical planning and suture patterns, it cannot account for active biological processes such as angiogenesis, inflammatory response, or axonal growth, which are critical for successful repair. Ultimately, this study provides a reliable anatomical platform for NGC evaluation under controlled experimental conditions, serving as a necessary precursor to in vivo validation of safety and functional outcomes. Full article

Polyurea, a novel spray-applied composite polymer, is of high application importance for rapid roadway support in coal mines. The current study investigates the dynamic process and mechanisms governing the impact and spreading of polyurea droplets on rough surfaces through experimental and theoretical approaches. The key novelty lies in revealing how spontaneously varying viscosity couples with surface microstructure to produce novel scaling laws distinct from classical Newtonian behavior. The droplet impact and wetting process can be divided into three stages. In the pinning stage, droplet behavior is dominated by kinetic energy, leading to inertia-driven spreading in which the contact line radius increases quite slowly with time. In the penetration stage, the apparent three-phase contact line (TPCL) is pinned by surface microstructures, while the real TPCL evolves with time following a temporal scaling law t^3/2. In the spreading stage, surface roughness becomes decisive. On low-roughness substrates, limited pinning allows the real and apparent TPCLs to spread synchronously, with TPCL evolution governed by surface tension and viscous forces, following a t^1/8 scaling law. As roughness increases, pinning effects strengthen, causing divergence: the real TPCL is driven by surface tension and viscous dissipation between microstructures, whereas the apparent TPCL is additionally influenced by pinning and reaction-induced viscosity, scaling as t^1/24. This t^1/24 scaling for the apparent contact line on relatively high-roughness surfaces represents a significant deviation from established scaling relations. Experiments on rock-like substrates confirm these mechanisms for polyurea droplets. These findings provide theoretical and engineering guidance for optimizing spray-coating parameters in coal mines, with the goal of improving coating uniformity and interfacial adhesion. Full article