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19 pages, 10210 KiB  
Article
Evaluating Landscape Fragmentation and Consequent Environmental Impact of Solar Parks Installation in Natura 2000 Protected Areas: The Case of the Thessaly Region, Central Greece
by Ioannis Faraslis, Vassiliki Margaritopoulou, Christos Christakis and Efthimios Providas
Sustainability 2025, 17(15), 7158; https://doi.org/10.3390/su17157158 (registering DOI) - 7 Aug 2025
Abstract
This study examines the adverse environmental impacts of solar photovoltaic parks located in established protected areas, aiming to determine the level of landscape fragmentation through the calculation of relevant landscape metrics. For this purpose, a case study was carried out in a Mediterranean [...] Read more.
This study examines the adverse environmental impacts of solar photovoltaic parks located in established protected areas, aiming to determine the level of landscape fragmentation through the calculation of relevant landscape metrics. For this purpose, a case study was carried out in a Mediterranean Natura 2000 Special Protection Area (SPA), and landscape metrics were calculated using Geographic Information System spatial analysis tools. The analysis of metrics showed that the installation of renewable energy parks within the designated protected area negatively affect landscape fragmentation and the absence of carefully defined and evidence-based mitigation measures. The land cover categories that are significantly affected are those considered critical habitats of bird species that have been designated as SPAs. The results of this study highlight the need to integrate, in the National Renewable Energy Spatial Plans, specific biodiversity objectives, such as conservation objectives and the suspension of the installation of photovoltaic parks in certain areas that are important for conservation of biodiversity, in order to ensure the overall sustainability of renewable energy production. Full article
(This article belongs to the Special Issue Environmental Protection and Sustainable Ecological Engineering)
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16 pages, 7600 KiB  
Article
Passive Long-Term Acoustic Sampling Reveals Multiscale Temporal Ecological Pattern and Anthropogenic Disturbance of Campus Forests in a High Density City
by Xiaoqing Xu, Xueyao Sun and Hanbin Xie
Forests 2025, 16(8), 1289; https://doi.org/10.3390/f16081289 - 7 Aug 2025
Abstract
Biodiversity conservation and sustainable development in high-density forest urban areas have attracted growing attention and are increasingly recognized as critical for achieving the Sustainable Development Goals (SDGs). University campus forests, functioning as ecological islands, possess unique acoustic characteristics and play a vital role [...] Read more.
Biodiversity conservation and sustainable development in high-density forest urban areas have attracted growing attention and are increasingly recognized as critical for achieving the Sustainable Development Goals (SDGs). University campus forests, functioning as ecological islands, possess unique acoustic characteristics and play a vital role in supporting urban biodiversity. In this case study, acoustic monitoring was conducted over the course of a full year to objectively reveal the ecological patterns across temporal scales of the campus sound environment, by combining acoustic indices’ visualization combined with statistical analysis. The findings indicate (1) the existence of ecological sound patterns across different temporal scales, closely associated with phenological cycles; (2) the identification of the specific timing affected by the different species‘ activities, such as the breeding season of birds, the chirping time of cicadas and other insects, as well as the fluctuations in the intensity of human activities, and (3) the development of a methodological framework integrating a visualization technique with statistical analysis to enhance the understanding of long-term ecological dynamics. The results offer a foundation for promoting the sustainable conservation of campus biodiversity in high-density urban settings. Full article
(This article belongs to the Special Issue Soundscape in Urban Forests—2nd Edition)
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23 pages, 273 KiB  
Article
Checklist of the Tribe Eucosmini Obraztsov, 1946 (Lepidoptera: Tortricidae: Olethreutinae) from Taiwan
by Yinghui Sun, John W. Brown, Ming Liu, Qiangcheng Zeng and Houhun Li
Insects 2025, 16(8), 819; https://doi.org/10.3390/insects16080819 - 7 Aug 2025
Abstract
This study presents an updated and detailed inventory of the tortricid tribe Eucosmini found in Taiwan Province, China, highlighting 26 genera and 53 species. Several taxonomic novelties were revealed: The genus Coenobiodes is newly recorded for Taiwan Province, Hermenias semicurva is newly reported [...] Read more.
This study presents an updated and detailed inventory of the tortricid tribe Eucosmini found in Taiwan Province, China, highlighting 26 genera and 53 species. Several taxonomic novelties were revealed: The genus Coenobiodes is newly recorded for Taiwan Province, Hermenias semicurva is newly reported for China, and six species are newly recorded for Taiwan. Most Eucosmini species in Taiwan are widespread in eastern Asia and/or the Palearctic, but 12 (23%) are endemic to Taiwan. Biogeographical distributions are provided for each species, and a list of the specimens examined is provided where applicable. These findings underscore Taiwan’s status as a biodiversity hotspot and offer crucial data for understanding regional and global biodiversity patterns. Full article
(This article belongs to the Section Insect Systematics, Phylogeny and Evolution)
19 pages, 4925 KiB  
Article
Environmental Heterogeneity Drives Diversity Across Forest Strata in Hopea hainanensis Communities
by Shaocui He, Donghai Li, Xiaobo Yang, Dongling Qi, Naiyan Shang, Caiqun Liang, Rentong Liu and Chunyan Du
Diversity 2025, 17(8), 556; https://doi.org/10.3390/d17080556 - 7 Aug 2025
Abstract
Species and phylogenetic diversity play vital roles in sustaining the structure, function, and resilience of plant communities, particularly in tropical rainforests. However, the mechanisms according to which environmental filtering and competitive exclusion influence diversity across forest layers remain insufficiently understood. In this study, [...] Read more.
Species and phylogenetic diversity play vital roles in sustaining the structure, function, and resilience of plant communities, particularly in tropical rainforests. However, the mechanisms according to which environmental filtering and competitive exclusion influence diversity across forest layers remain insufficiently understood. In this study, we investigated the species and phylogenetic diversity patterns in two representative tropical rainforest sites—Bawangling and Jianfengling—within Hainan Tropical Rainforest National Park, China, focusing on communities associated with the endangered species Hopea hainanensis. We employed a one-way ANOVA and Pearson’s correlation analyses to examine the distribution characteristics and interrelationships among diversity indices and used Mantel tests to assess the correlations with environmental variables. Our results revealed that the plant community in Jianfengling exhibited a significantly higher species richness at the family, genus, and species levels (a total of 288 plant species have been recorded, belonging to 82 families and 183 genera) compared to that in Bawangling (a total of 212 plant species, belonging to 75 families and 162 genera). H. hainanensis held the highest importance value in the middle tree layer across both sites (IV(BWL) = 12.44; IV(JFL) = 5.73), while dominant species varied notably among other forest layers, indicating strong habitat specificity. Diversity indices, including the Simpson index, the Shannon–Wiener index, and Pielou’s evenness, were significantly higher in the large shrub layer of Jianfengling, whereas Bawangling showed a relatively higher Shannon–Wiener index in the middle shrub layer. Phylogenetic diversity (PD) and the phylogenetic structure indices (NRI and NTI) displayed distinct vertical stratification patterns between sites. Furthermore, the PD in Bawangling’s large shrub layer was positively correlated with total phosphorus in the soil, while community evenness was influenced by soil organic carbon and total nitrogen. In Jianfengling, species richness was significantly associated with soil bulk density, altitude, and pH. These findings enhance our understanding of the ecological and evolutionary processes shaping biodiversity in tropical rainforests and highlight the importance of incorporating both species and phylogenetic metrics into the conservation strategies for endangered species such as Hopea hainanensis. Full article
(This article belongs to the Special Issue Biodiversity Conservation Planning and Assessment—2nd Edition)
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20 pages, 2079 KiB  
Article
Spatiotemporal Patterns of Avian Species Richness Across Climatic Regions
by Çağdan Uyar, Serkan Özdemir, Dalia Perkumienė, Marius Aleinikovas, Benas Šilinskas and Mindaugas Škėma
Diversity 2025, 17(8), 557; https://doi.org/10.3390/d17080557 - 7 Aug 2025
Abstract
This study highlights the spatial, seasonal, and climatic variations in bird species richness across Türkiye, a country with rich avian richness situated at the intersection of major migratory routes. Bird species richness was calculated for each province. Differences between regions, Köppen–Geiger climate classes, [...] Read more.
This study highlights the spatial, seasonal, and climatic variations in bird species richness across Türkiye, a country with rich avian richness situated at the intersection of major migratory routes. Bird species richness was calculated for each province. Differences between regions, Köppen–Geiger climate classes, and seasons were analyzed using the Kruskal–Wallis method. Non-parametric analysis of longitudinal data in factorial experiments was also employed to determine seasonal differences within regions and climate classes. The results revealed significant spatial variations in species richness, particularly between temperate and cold climate regions. While seasonal differences were generally less pronounced, they were critical for both migratory and resident bird species. Wetlands, coastal areas, and transitional habitats were identified as biodiversity hotspots for both resident and migratory birds. This study underscores the need to integrate regional, climatic, and seasonal variations into ecosystem-based management plans. Protecting critical habitats, enhancing connectivity through ecological corridors, and adopting adaptive conservation strategies are essential for sustaining Türkiye’s rich avian diversity. These results provide valuable insights for conservation planning and emphasize the importance of addressing spatial and seasonal dynamics to ensure long-term biodiversity preservation. Full article
(This article belongs to the Special Issue Diversity in 2025)
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20 pages, 3615 KiB  
Article
Identification of Suitable Habitats for Threatened Elasmobranch Species in the OSPAR Maritime Area
by Moritz Mercker, Miriam Müller, Thorsten Werner and Janos Hennicke
Fishes 2025, 10(8), 393; https://doi.org/10.3390/fishes10080393 - 7 Aug 2025
Abstract
Protecting threatened elasmobranch species despite limited data on their distribution and abundance is a critical challenge, particularly in the context of increasing human impacts on marine ecosystems. In the northeastern Atlantic, species such as the leafscale gulper shark, Portuguese dogfish, spurdog, and spotted [...] Read more.
Protecting threatened elasmobranch species despite limited data on their distribution and abundance is a critical challenge, particularly in the context of increasing human impacts on marine ecosystems. In the northeastern Atlantic, species such as the leafscale gulper shark, Portuguese dogfish, spurdog, and spotted ray are facing pressures from overfishing, bycatch, habitat degradation, and climate change. The OSPAR Commission has listed these species as threatened and/or declining and aims to protect them by reliably identifying suitable habitats and integrating these areas into Marine Protected Areas (MPAs). In this study, we present a spatial modelling framework using regression-based approaches to identify suitable habitats for these four species. Results show that suitable habitats of the spotted ray (25.8%) and spurdog (18.8%) are relatively well represented within existing MPAs, while those of the deep-water sharks are underrepresented (6.0% for leafscale gulper shark, and 6.8% for Portuguese dogfish). Our findings highlight the need for additional MPAs in deep-sea continental slope areas, particularly west and northwest of Scotland and Ireland. Such expansions would support OSPAR’s goal to protect 30% of its maritime area by 2030 and could benefit broader deep-sea biodiversity, including other vulnerable demersal species and benthic communities. Full article
(This article belongs to the Special Issue Habitat Assessment and Conservation of Fishes)
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15 pages, 1458 KiB  
Article
Effect of Precipitation Change on Desert Steppe Aboveground Productivity
by Yonghong Luo, Jiming Cheng, Ziyu Cao, Haixiang Zhang, Pengcuo Danba, Jiazhi Wang, Ying Wang, Rong Zhang, Chao Zhang, Yingqun Feng and Shuhua Wei
Biology 2025, 14(8), 1010; https://doi.org/10.3390/biology14081010 - 6 Aug 2025
Abstract
Precipitation changes have significant impacts on biodiversity and ecosystem productivity. However, the effects of precipitation changes on species diversity have been the focus of most previous studies. Little is known about the contributions of different dimensions of biodiversity (species, functional, and phylogenetic diversity) [...] Read more.
Precipitation changes have significant impacts on biodiversity and ecosystem productivity. However, the effects of precipitation changes on species diversity have been the focus of most previous studies. Little is known about the contributions of different dimensions of biodiversity (species, functional, and phylogenetic diversity) in linking long-term precipitation changes to ecosystem functions. In this study, a randomized design was conducted in the desert steppes of Ningxia, which included three treatments: natural rainfall, precipitation reduced by 50%, and precipitation increased by 50%. After 4 years of treatment, the effects of precipitation changes on aboveground productivity and its underlying mechanisms were explored. The results showed that (1) reduced precipitation significantly decreased phylogenetic diversity and species diversity, but had no significant effect on functional diversity; (2) reduced precipitation significantly decreased aboveground productivity, while increased precipitation significantly enhanced aboveground productivity; and (3) changes in precipitation primarily regulated aboveground productivity by altering soil nitrogen availability and the size of dominant plant species. This study provides important theoretical and practical guidance for the protection and management of desert steppe vegetation under future climate change. Full article
(This article belongs to the Section Ecology)
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14 pages, 7789 KiB  
Article
Integrated Sampling Approaches Enhance Assessment of Saproxylic Beetle Biodiversity in a Mediterranean Forest Ecosystem (Sila National Park, Italy)
by Federica Mendicino, Francesco Carlomagno, Domenico Bonelli, Erica Di Biase, Federica Fumo and Teresa Bonacci
Insects 2025, 16(8), 812; https://doi.org/10.3390/insects16080812 - 6 Aug 2025
Abstract
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase [...] Read more.
Saproxylic beetles are key bioindicators of forest ecosystem quality and play essential roles in deadwood decomposition and nutrient cycling. However, their populations are increasingly threatened by habitat fragmentation, deadwood removal, and climate-driven environmental changes. For this reason, an integrated sampling method can increase the detection of species with varying ecological traits. We evaluated the effectiveness of integrative sampling methodologies to assess saproxylic beetle diversity within Sila National Park, a Mediterranean forest ecosystem of high conservation value, specifically in two beech forests and four pine forests. The sampling methods tested included Pan Traps (PaTs), Malaise Traps (MTs), Pitfall Traps (PTs), Bait Bottle Traps (BBTs), and Visual Census (VC). All specimens were identified to the species level whenever possible, using specialized dichotomous keys and preserved in the Entomological Collection TB, Unical. Various trap types captured a different number of species: the PaT collected 32 species, followed by the PT with 24, the MT with 16, the VC with 7, and the BBT with 5 species. Interestingly, biodiversity analyses conducted using PAST software version 4.17 revealed that PaTs and MTs recorded the highest biodiversity indices. The GLMM analysis, performed using SPSS software 29.0.1.0, demonstrated that various traps attracted different species with different abundances. By combining multiple trapping techniques, we documented a more comprehensive community composition compared to single-method approaches. Moreover, PaTs, MTs, and PTs recorded 20%, 40%, and 33% of the Near Threatened species, respectively. We report new records for Sila National Park, including the LC species Pteryngium crenulatum (Curculionidae) and the NT species Grynocharis oblonga (Trogossitidae). For the first time in Calabria, the LC species Triplax rufipes (Erotylidae) and the NT species Oxypleurus nodieri (Cerambycidae) and Glischrochilus quadrisignatus (Nitidulidae) were collected. Our results emphasize the importance of method diversity in capturing species with distinct ecological requirements and highlight the relevance of saproxylic beetles as indicators of forest health. These findings support the adoption of multi-method sampling protocols in forest biodiversity monitoring and management programs, especially in biodiversity-rich and structurally heterogeneous landscapes. Full article
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25 pages, 4069 KiB  
Article
Forest Volume Estimation in Secondary Forests of the Southern Daxing’anling Mountains Using Multi-Source Remote Sensing and Machine Learning
by Penghao Ji, Wanlong Pang, Rong Su, Runhong Gao, Pengwu Zhao, Lidong Pang and Huaxia Yao
Forests 2025, 16(8), 1280; https://doi.org/10.3390/f16081280 - 5 Aug 2025
Abstract
Forest volume is an important information for assessing the economic value and carbon sequestration capacity of forest resources and serves as a key indicator for energy flow and biodiversity. Although remote sensing technology is applied to estimate volume, optical remote sensing data have [...] Read more.
Forest volume is an important information for assessing the economic value and carbon sequestration capacity of forest resources and serves as a key indicator for energy flow and biodiversity. Although remote sensing technology is applied to estimate volume, optical remote sensing data have limitations in capturing forest vertical height information and may suffer from reflectance saturation. While LiDAR data can provide more detailed vertical structural information, they come with high processing costs and limited observation range. Therefore, improving the accuracy of volume estimation through multi-source data fusion has become a crucial challenge and research focus in the field of forest remote sensing. In this study, we integrated Sentinel-2 multispectral data, Resource-3 stereoscopic imagery, UAV-based LiDAR data, and field survey data to quantitatively estimate the forest volume in Saihanwula Nature Reserve, located in Inner Mongolia, China, on the southern part of Daxing’anling Mountains. The study evaluated the performance of multi-source remote sensing features by using recursive feature elimination (RFE) to select the most relevant factors and applied four machine learning models—multiple linear regression (MLR), k-nearest neighbors (kNN), random forest (RF), and gradient boosting regression tree (GBRT)—to develop volume estimation models. The evaluation metrics include the coefficient of determination (R2), root mean square error (RMSE), and relative root mean square error (rRMSE). The results show that (1) forest Canopy Height Model (CHM) data were strongly correlated with forest volume, helping to alleviate the reflectance saturation issues inherent in spectral texture data. The fusion of CHM and spectral data resulted in an improved volume estimation model with R2 = 0.75 and RMSE = 8.16 m3/hm2, highlighting the importance of integrating multi-source canopy height information for more accurate volume estimation. (2) Volume estimation accuracy varied across different tree species. For Betula platyphylla, we obtained R2 = 0.71 and RMSE = 6.96 m3/hm2; for Quercus mongolica, R2 = 0.74 and RMSE = 6.90 m3/hm2; and for Populus davidiana, R2 = 0.51 and RMSE = 9.29 m3/hm2. The total forest volume in the Saihanwula Reserve ranges from 50 to 110 m3/hm2. (3) Among the four machine learning models, GBRT consistently outperformed others in all evaluation metrics, achieving the highest R2 of 0.86, lowest RMSE of 9.69 m3/hm2, and lowest rRMSE of 24.57%, suggesting its potential for forest biomass estimation. In conclusion, accurate estimation of forest volume is critical for evaluating forest management practices and timber resources. While this integrated approach shows promise, its operational application requires further external validation and uncertainty analysis to support policy-relevant decisions. The integration of multi-source remote sensing data provides valuable support for forest resource accounting, economic value assessment, and monitoring dynamic changes in forest ecosystems. Full article
(This article belongs to the Special Issue Mapping and Modeling Forests Using Geospatial Technologies)
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14 pages, 3486 KiB  
Article
Spatiotemporal Activity Patterns of Sympatric Rodents and Their Predators in a Temperate Desert-Steppe Ecosystem
by Caibo Wei, Yijie Ma, Yuquan Fan, Xiaoliang Zhi and Limin Hua
Animals 2025, 15(15), 2290; https://doi.org/10.3390/ani15152290 - 5 Aug 2025
Abstract
Understanding how prey and predator species partition activity patterns across time and space is essential for elucidating behavioral adaptation and ecological coexistence. In this study, we examined the diel and seasonal activity rhythms of two sympatric rodent species—Rhombomys opimus (Great gerbil) and [...] Read more.
Understanding how prey and predator species partition activity patterns across time and space is essential for elucidating behavioral adaptation and ecological coexistence. In this study, we examined the diel and seasonal activity rhythms of two sympatric rodent species—Rhombomys opimus (Great gerbil) and Meriones meridianus (Midday gerbil)—and their primary predators, Otocolobus manul (Pallas’s cat) and Vulpes vulpes (Red fox), in a desert-steppe ecosystem on the northern slopes of the Qilian Mountains, China. Using over 8000 camera trap days and kernel density estimation, we quantified their activity intensity and spatiotemporal overlap. The two rodent species showed clear temporal niche differentiation but differed in their synchrony with predators. R. opimus exhibited a unimodal diurnal rhythm with spring activity peaks, while M. meridianus showed stable nocturnal activity with a distinct autumn peak. Notably, O. manul adjusted its activity pattern to partially align with that of R. opimus, whereas V. vulpes maintained a crepuscular–nocturnal rhythm overlapping more closely with that of M. meridianus. Despite distinct temporal rhythms, both rodent species shared high spatial overlap with their predators (overlap index OI = 0.64–0.83). These findings suggest that temporal partitioning may reduce predation risk for R. opimus, while M. meridianus co-occurs more extensively with its predators. Our results highlight the ecological role of native carnivores in rodent population dynamics and support their potential use in biodiversity-friendly rodent management strategies under arid grassland conditions. Full article
(This article belongs to the Section Ecology and Conservation)
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19 pages, 4059 KiB  
Article
Vulnerability Assessment of Six Endemic Tibetan-Himalayan Plants Under Climate Change and Human Activities
by Jin-Dong Wei and Wen-Ting Wang
Plants 2025, 14(15), 2424; https://doi.org/10.3390/plants14152424 - 5 Aug 2025
Abstract
The Tibetan-Himalayan region, recognized as a global biodiversity hotspot, is increasingly threatened by the dual pressures of climate change and human activities. Understanding the vulnerability of plant species to these forces is crucial for effective ecological conservation in this region. This study employed [...] Read more.
The Tibetan-Himalayan region, recognized as a global biodiversity hotspot, is increasingly threatened by the dual pressures of climate change and human activities. Understanding the vulnerability of plant species to these forces is crucial for effective ecological conservation in this region. This study employed an improved Climate Niche Factor Analysis (CNFA) framework to assess the vulnerability of six representative alpine endemic herbaceous plants in this ecologically sensitive region under future climate changes. Our results show distinct spatial vulnerability patterns for the six species, with higher vulnerability in the western regions of the Tibetan-Himalayan region and lower vulnerability in the eastern areas. Particularly under high-emission scenarios (SSP5-8.5), climate change is projected to substantially intensify threats to these plant species, reinforcing the imperative for targeted conservation strategies. Additionally, we found that the current coverage of protected areas (PAs) within the species’ habitats was severely insufficient, with less than 25% coverage overall, and it was even lower (<7%) in highly vulnerable regions. Human activity hotspots, such as the regions around Lhasa and Chengdu, further exacerbate species vulnerability. Notably, some species currently classified as least concern (e.g., Stipa purpurea (S. purpurea)) according to the IUCN Red List exhibit higher vulnerability than species listed as near threatened (e.g., Cyananthus microphyllus (C. microphylla)) under future climate change. These findings suggest that existing biodiversity assessments, such as the IUCN Red List, may not adequately account for future climate risks, highlighting the importance of incorporating climate change projections into conservation planning. Our study calls for expanding and optimizing PAs, improving management, and enhancing climate resilience to mitigate biodiversity loss in the face of climate change and human pressures. Full article
(This article belongs to the Section Plant Ecology)
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19 pages, 3457 KiB  
Article
Transcriptome Analysis Revealed the Immune and Metabolic Responses of Grass Carp (Ctenopharyngodon idellus) Under Acute Salinity Stress
by Leshan Ruan, Baocan Wei, Yanlin Liu, Rongfei Mu, Huang Li and Shina Wei
Fishes 2025, 10(8), 380; https://doi.org/10.3390/fishes10080380 - 5 Aug 2025
Viewed by 135
Abstract
Freshwater salinization, an escalating global environmental stressor, poses a significant threat to freshwater biodiversity, including fish communities. This study investigates the grass carp (Ctenopharyngodon idellus), a species with the highest aquaculture output in China, to elucidate the molecular underpinnings of its [...] Read more.
Freshwater salinization, an escalating global environmental stressor, poses a significant threat to freshwater biodiversity, including fish communities. This study investigates the grass carp (Ctenopharyngodon idellus), a species with the highest aquaculture output in China, to elucidate the molecular underpinnings of its physiological adaptations to fluctuating salinity gradients. We used high-throughput mRNA sequencing and differential gene expression profiling to analyze transcriptional dynamics in intestinal and kidney tissues of grass carp exposed to heterogeneous salinity stressors. Concurrent serum biochemical analyses showed salinity stress significantly increased Na+, Cl, and osmolarity, while decreasing lactate and glucose. Salinity stress exerted a profound impact on the global transcriptomic landscape of grass carp. A substantial number of co-regulated differentially expressed genes (DEGs) in kidney and intestinal tissues were enriched in immune and metabolic pathways. Specifically, genes associated with antigen processing and presentation (e.g., cd4-1, calr3b) and apoptosis (e.g., caspase17, pik3ca) exhibited upregulated expression, whereas genes involved in gluconeogenesis/glycolysis (e.g., hk2, pck2) were downregulated. KEGG pathway enrichment analyses revealed that metabolic and cellular structural pathways were predominantly enriched in intestinal tissues, while kidney tissues showed preferential enrichment of immune and apoptotic pathways. Rigorous validation of RNA-seq data via qPCR confirmed the robustness and cross-platform consistency of the findings. This study investigated the core transcriptional and physiological mechanisms regulating grass carp’s response to salinity stress, providing a theoretical foundation for research into grass carp’s resistance to salinity stress and the development of salt-tolerant varieties. Full article
(This article belongs to the Special Issue Adaptation and Response of Fish to Environmental Changes)
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11 pages, 1381 KiB  
Article
Fertilization Promotes the Recovery of Plant Productivity but Decreases Biodiversity in a Khorchin Degraded Grassland
by Lina Zheng, Wei Zhao, Shaobo Gao, Ruizhen Wang, Haoran Yan and Mingjiu Wang
Nitrogen 2025, 6(3), 64; https://doi.org/10.3390/nitrogen6030064 - 4 Aug 2025
Viewed by 64
Abstract
Fertilization is a critical measure for vegetation restoration and ecological reconstruction in degraded grasslands. However, little is known about the long-term effects of different combinations of nitrogen (N), phosphorus (P), potassium (K) on plant and microbial communities in degraded grasslands. This study conducted [...] Read more.
Fertilization is a critical measure for vegetation restoration and ecological reconstruction in degraded grasslands. However, little is known about the long-term effects of different combinations of nitrogen (N), phosphorus (P), potassium (K) on plant and microbial communities in degraded grasslands. This study conducted a four-year (2017–2020) N, P, K addition experiment in the Khorchin Grassland, a degraded typical grassland located in Zhalute Banner, Tongliao City, Inner Mongolia, to investigate the effects of fertilization treatment on plant functional groups and microbial communities after grazing exclusion. Our results showed that the addition of P, NP, and NPK compound fertilizers significantly increased aboveground biomass of the plant community, which is mainly related to the improvement of nutrient availability to promote the growth of specific plant functional groups, especially annual and biennial plants and perennial bunchgrasses. However, the addition of N, P, and NP fertilizers significantly reduced the species diversity of the plant community. At the same time, the addition of N, P, and NP fertilizers and the application of N and NP significantly reduced fungal species diversity but had no significant effect on soil bacteria. Our study provides new insights into the relationships between different types of fertilization and plant community productivity and biodiversity in degraded grasslands over four years of fertilization, which is critical for evaluating the effect of fertilization on the restoration of degraded grassland. Full article
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20 pages, 3354 KiB  
Article
An Assessment of the Population Structure and Stock Dynamics of Megalobrama skolkovii During the Early Phase of the Fishing Ban in the Poyang Lake Basin
by Xinwen Huang, Qun Xu, Bao Zhang, Chiping Kong, Lei Fang, Xiaoping Gao, Leyi Sun, Lekang Li and Xiaoling Gong
Fishes 2025, 10(8), 378; https://doi.org/10.3390/fishes10080378 - 4 Aug 2025
Viewed by 90
Abstract
The ten-year fishing ban on the Yangtze River aims to restore aquatic biodiversity and rebuild fishery resources. Megalobrama skolkovii, a key species in the basin, was investigated using 2024 data to provide a preliminary assessment of its population structure, stock dynamics, and [...] Read more.
The ten-year fishing ban on the Yangtze River aims to restore aquatic biodiversity and rebuild fishery resources. Megalobrama skolkovii, a key species in the basin, was investigated using 2024 data to provide a preliminary assessment of its population structure, stock dynamics, and early recovery. Age analysis (n = 243) showed that 1–6-year-olds were dominated by fish aged 3 (35%), with few older than 4, indicating moderate structural truncation. Growth parameters modeled by the von Bertalanffy Growth Function yielded L = 61.89 cm and k = 0.25 year1, with a weight–growth inflection age of 4.4 years. Natural mortality (M = 0.48 year−1) was estimated using Pauly’s empirical formula, and total mortality (Z = 0.55 year−1) was estimated from the catch curve analysis. While fishing mortality (F) was statistically indistinguishable from zero, a plausible low-intensity fishing scenario was explored to assess potential impacts of residual activities. Length-based indicators (LBIs) showed Pmat = 46.05%, Popt = 9.51%, and Pmega = 6.88%, suggesting reproductive recovery but incomplete structural restoration. These preliminary findings reveal an asymmetrical recovery trajectory, whereby physiological improvements and enhanced recruitment have occurred, yet full structural restoration remains incomplete. This underscores the need for continued, long-term conservation and monitoring to support population resilience. Full article
(This article belongs to the Section Biology and Ecology)
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31 pages, 2983 KiB  
Review
Sustainable Management of Willow Forest Landscapes: A Review of Ecosystem Functions and Conservation Strategies
by Florin Achim, Lucian Dinca, Danut Chira, Razvan Raducu, Alexandru Chirca and Gabriel Murariu
Land 2025, 14(8), 1593; https://doi.org/10.3390/land14081593 - 4 Aug 2025
Viewed by 160
Abstract
Willow stands (Salix spp.) are an essential part of riparian ecosystems, as they sustain biodiversity and provide bioenergy solutions. The present review synthesizes the global scientific literature about the management of willow stands. In order to achieve this goal, we used a [...] Read more.
Willow stands (Salix spp.) are an essential part of riparian ecosystems, as they sustain biodiversity and provide bioenergy solutions. The present review synthesizes the global scientific literature about the management of willow stands. In order to achieve this goal, we used a dual approach combining bibliometric analysis with traditional literature review. As such, we consulted 416 publications published between 1978 and 2024. This allowed us to identify key species, ecosystem services, conservation strategies, and management issues. The results we have obtained show a diversity of approaches, with an increase in short-rotation coppice (SRC) systems and the multiple roles covered by willow stands (carbon sequestration, biomass production, riparian restoration, and habitat provision). The key trends we have identified show a shift toward topics such as climate resilience, ecological restoration, and precision forestry. This trend has become especially pronounced over the past decade (2014–2024), as reflected in the increasing use of these keywords in the literature. However, as willow systems expand in scale and function—from biomass production to ecological restoration—they also raise complex challenges, including invasive tendencies in non-native regions and uncertainties surrounding biodiversity impacts and soil carbon dynamics over the long term. The present review is a guide for forest policies and, more specifically, for future research, linking the need to integrate and use adaptive strategies in order to maintain the willow stands. Full article
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