Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (194)

Search Parameters:
Keywords = soybean substrate

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
16 pages, 5397 KB  
Article
The Agronomic Traits Differences in Hericium erinaceus Cultivated with Different Straw Formulations by Replacing Wood with Straw
by Zhu Lu, Yang Yang, Shuang Hu, Yu-Kun Ma, Zi-Ming Ren, Yue Wang, Ying-Kun Yang, Shu-Juan Ji, Huan Wang and Xiao Huang
Horticulturae 2025, 11(10), 1220; https://doi.org/10.3390/horticulturae11101220 - 10 Oct 2025
Viewed by 241
Abstract
Hericium erinaceus, a rare edible–medicinal fungus, has attracted great attention in food and pharmaceutical fields due to its rich nutritional and bioactive components. However, its traditional cultivation relies heavily on wood chip substrates, causing resource unsustainability. The “wood-replacing-with-grass” technology can address this [...] Read more.
Hericium erinaceus, a rare edible–medicinal fungus, has attracted great attention in food and pharmaceutical fields due to its rich nutritional and bioactive components. However, its traditional cultivation relies heavily on wood chip substrates, causing resource unsustainability. The “wood-replacing-with-grass” technology can address this issue, contributing to ecological conservation and alleviating resource conflicts between edible fungus cultivation and forestry development. This study focused on straw substitution for wood chips, initially screening suitable straw types and optimal addition ratios from 7 straw varieties, and systematically investigating the agronomic trait variations in H. erinaceus under different substrate formulations via cultivation experiments. Results showed the following: (1) Rapeseed straw, soybean straw, and corn straw substituting 20%, 30%, and 40% of wood chips, respectively, promoted better mycelial growth of H. erinaceus. (2) All screened straw formulations enabled fruiting. With increased straw addition, the mycelial full colonization time shortened (up to 5 days shorter in 40% corn/soybean straw treatments). The 20% corn straw treatment showed significantly higher biological efficiency and average fresh weight than the control (CK); the 20% soybean straw treatment had no significant difference in biological efficiency but significantly higher average fresh weight than CK; and the 20% rapeseed straw treatment showed no significant differences in both indexes from CK. However, when straw addition exceeded 20%, fruiting body firmness, yield, and biological efficiency decreased progressively. (3) The 40% soybean straw treatment yielded fruiting bodies with the highest crude protein, manganese, and iron contents, while the 40% rapeseed straw treatment had the highest crude fat, potassium, phosphorus, calcium, zinc, and selenium contents. These findings provide a theoretical basis and practical reference for optimizing H. erinaceus cultivation substrate formulations, improving product quality, and promoting sustainable industrial development. Full article
(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)
Show Figures

Figure 1

17 pages, 2783 KB  
Article
From Isolation to Plant Growth Evaluation: Development of a Streptomyces-Based Bioinput Using Spent Yeast and Composting Leachate
by Mateus Torres Nazari, Aline Rubert, Vera Analise Schommer, Bruna Strieder Machado, Camila Vancini, Daniela Dal Castel Krein, Valdecir Ferrari, Helen Treichel, Luciane Maria Colla and Jeferson Steffanello Piccin
Fermentation 2025, 11(10), 556; https://doi.org/10.3390/fermentation11100556 - 27 Sep 2025
Viewed by 462
Abstract
This study aimed to develop a sustainable bioinput using Streptomyces sp. BEI-18A cultivated in an alternative culture medium (ACM) formulated with winery spent yeast and composting leachate. Actinomycetes were initially isolated from grape waste composting piles and screened for agricultural potential in vitro. [...] Read more.
This study aimed to develop a sustainable bioinput using Streptomyces sp. BEI-18A cultivated in an alternative culture medium (ACM) formulated with winery spent yeast and composting leachate. Actinomycetes were initially isolated from grape waste composting piles and screened for agricultural potential in vitro. Streptomyces sp. BEI-18A was selected for further bioinput development based on its high siderophore production. The ACM formulation was optimized in three steps: (I) determining the optimal concentration of winery spent yeast through mixture design; (II) assessing the effect of composting leachate addition on microbial growth; and (III) establishing the final composition of ACM components. The optimized ACM consisted of 3 g/L spent yeast, 2 g/L sucrose, 1 g/L soybean extract, and 10% (v/v) composting leachate. Cultivation of Streptomyces sp. BEI-18A in this medium resulted in a bioinput containing 7.80 × 107 CFU/mL. Its agricultural potential was validated in pot experiments with wheat and soybean, where application of the bioinput promoted significant improvements in early plant growth, enhancing several phytometric parameters. The results highlight the feasibility of valorizing agro-industrial residues as low-cost substrates for microbial bioinput production. This approach represents a promising strategy to foster sustainability in agriculture while reducing environmental impacts. Full article
Show Figures

Figure 1

15 pages, 2564 KB  
Article
Optimizing Pleurotus ostreatus Mushroom Cultivation on Various Agro-Industrial By-Products—Development of a Process Analytical Technology Tool for Predicting Biological Efficiency
by Georgios Bekiaris, Christos S. Pappas, Athanasios Mastrogiannis, Lefteris Lachouvaris, Petros A. Tarantilis and Georgios I. Zervakis
Fermentation 2025, 11(10), 555; https://doi.org/10.3390/fermentation11100555 - 27 Sep 2025
Viewed by 592
Abstract
Pleurotus ostreatus is among the most widely cultivated mushroom species on a global scale, valued for its relative ease of cultivation, excellent organoleptic qualities, and notable nutraceutical properties. P. ostreatus could use a wide range of by-products as growth substrates by excreting a [...] Read more.
Pleurotus ostreatus is among the most widely cultivated mushroom species on a global scale, valued for its relative ease of cultivation, excellent organoleptic qualities, and notable nutraceutical properties. P. ostreatus could use a wide range of by-products as growth substrates by excreting a potent array of hydrolytic and oxidative enzymes. In this study, a diverse range of agricultural residues and agro-industrial by-products, enriched (or not) with various supplements, was evaluated for the cultivation of five commercial P. ostreatus strains. Key cultivation parameters were assessed, including biological efficiency and productivity. A process analytical technology (PAT) approach, utilizing FTIR spectroscopy in combination with multivariate analysis, was employed to develop a predictive model for biological efficiency based solely on substrate’s spectral profile. Substrates based on wheat and barley straw supplemented with soybean demonstrated superior performance across most strains. The biological efficiency value reached 185% in some cases for a total cultivation period of only 35 days. The resulting model exhibited excellent predictive capability, with a coefficient of determination (R2) of 0.90 and low relative prediction error of just 6%. The developed innovative PAT tool will be beneficial for mushroom growers since it allows the fast and costless evaluation of agro-industrial by-products in respect to their potential exploitation as mushroom substrates. Full article
(This article belongs to the Special Issue Application of Fungi in Bioconversions and Mycoremediation)
Show Figures

Figure 1

17 pages, 571 KB  
Article
Malted Soybeans as a Substrate for Plant-Based Beverages—Analysis of Nutritional Properties, Antioxidant Activity, and Volatiles
by Ewelina Opiela, Anna Czubaszek, Alan Gasiński, Joanna Miedzianka and Joanna Kawa-Rygielska
Molecules 2025, 30(19), 3845; https://doi.org/10.3390/molecules30193845 - 23 Sep 2025
Viewed by 296
Abstract
Soybeans are often used as a raw material for the production of plant-based beverages. Malting significantly changes the properties of malted seeds; therefore, the aim of this study was the assessment of beverages obtained from soy malts (two types: ‘Pilsener’ and ‘Caramel’) produced [...] Read more.
Soybeans are often used as a raw material for the production of plant-based beverages. Malting significantly changes the properties of malted seeds; therefore, the aim of this study was the assessment of beverages obtained from soy malts (two types: ‘Pilsener’ and ‘Caramel’) produced from three soy varieties (Abaca, Abelina, and Aurelina). Beverages produced from malts were characterized by a higher protein content compared with beverages from unmalted seeds. The control samples showed a lower content of the sum of all amino acids (363.89–371.04 mg/g) compared with beverages from both types of malts, and the highest content was determined in the beverage from caramel-type malt of the Abaca variety (434.60 mg/g). Beverages from caramel-type malt of the Abaca and Aurelina varieties contained the largest concentration of phenolic compounds (8.35–10.33 mg GAE/100 mL) and the highest antioxidant activity (ABTS•+ 0.36–0.45 μmol Trolox/mL, FRAP 0.24–0.30 μmol Trolox/mL, and DPPH 0.08–0.09 μmol Trolox/mL). Analysis of the concentration of volatile compounds has shown that using malted soybeans had a significant effect on the composition and share of various groups of volatile compounds (aldehydes, alcohols, terpenes, and ketones) in the analyzed beverages. The obtained results indicate the possibility of using soy malt in the production of plant-based beverages. However, further work is necessary to improve the quality and organoleptic properties of these products. Full article
Show Figures

Figure 1

12 pages, 230 KB  
Article
Effect of Supplementing Lysine and Isoleucine as Limiting Amino Acids on Growth Performance and Amino Acid Deposition of Tenebrio molitor Larvae Reared on a Cereal-Based Substrate
by Guillermo Fondevila, Habib Fatmi, Pilar Fernando, Carlos Dapoza and Manuel Fondevila
Insects 2025, 16(9), 989; https://doi.org/10.3390/insects16090989 - 22 Sep 2025
Viewed by 420
Abstract
The effects of Lys and Ile supplementation in a barley-based substrate on growth performance and nutrient deposition in Tenebrio molitor larvae were evaluated. Six trays per treatment containing sixty larvae were assigned to five treatments in two 21-day periods: barley (B); an 85% [...] Read more.
The effects of Lys and Ile supplementation in a barley-based substrate on growth performance and nutrient deposition in Tenebrio molitor larvae were evaluated. Six trays per treatment containing sixty larvae were assigned to five treatments in two 21-day periods: barley (B); an 85% barley and 15% soybean meal mixture (BS) and B supplemented with synthetic Lys (BL), Ile (BI) or a combination of both (BLI), as potential limiting amino acids (AA) for larval growth. Supplementing AAs was provided in agar cubes twice per week. Larval performance was measured, and larvae were analyzed for nutrient content and AA profile. Final larval weight increased (p = 0.042) in BS and BL compared to B, with intermediate values for BI and BLI. Larval contents (mg/larva) in total AAs and seven key AAs (sum of Lys, Met, Cys, Thr, Trp, Val and Ile) were higher in BS-fed larvae than in BL and BLI, with the lowest values for BI and B (p < 0.001). Barley supplementation with Lys improved larval AA content, although the levels remained lower than those observed with BS. Conversely, Ile supplementation had no apparent effect on overall AA deposition compared to Lys alone, suggesting the presence of additional limiting AAs for optimal larval growth. Full article
(This article belongs to the Section Insect Physiology, Reproduction and Development)
16 pages, 73509 KB  
Article
GsCYP93D1, a Cytochrome P450 Gene from Wild Soybean, Mediates the Regulation of Plant Alkaline Tolerance and ABA Sensitivity
by Chao Chen, Jianyue Dai, Nuo Xu, Wanying Zhou, Liankun Xu, Qiuying Pang, Huizi Duanmu and Haiying Li
Plants 2025, 14(17), 2623; https://doi.org/10.3390/plants14172623 - 23 Aug 2025
Viewed by 515
Abstract
Cytochrome P450 enzymes (CYPs) are crucial catalysts responsible for the oxidative modification of diverse substrates, including plant hormones, antioxidants, and compounds involved in abiotic stress responses. While CYP functions in drought and salt stress adaptation have been extensively studied, their contribution to alkaline [...] Read more.
Cytochrome P450 enzymes (CYPs) are crucial catalysts responsible for the oxidative modification of diverse substrates, including plant hormones, antioxidants, and compounds involved in abiotic stress responses. While CYP functions in drought and salt stress adaptation have been extensively studied, their contribution to alkaline stress tolerance, particularly concerning specific cytochrome P450 genes in wild soybean (Glycine soja), remains less explored. In this study, a cytochrome P450 gene, GsCYP93D1, was identified and isolated, and its regulatory role under alkaline stress was elucidated. Transgenic GsCYP93D1 increased Arabidopsis and soybean hairy root resistance to alkaline stress, but the Arabidopsis atcyp93d1 mutant showed a reduced capacity for alkaline tolerance. Subsequent investigation showed the enhanced antioxidant defense capabilities of GsCYP93D1 transgenic plants, as evidenced by reduced superoxide radical (O2) production under exposure to alkaline stress. Furthermore, compared to the atcyp93d1 mutant, transgenic lines of GsCYP93D1 showed sensitivity to ABA. Moreover, transcript levels of genes associated with alkaline stress response and ABA signaling pathways were elevated in both GsCYP93D1 transgenic and mutant lines. Collectively, our findings demonstrate that GsCYP93D1 positively modulates plant tolerance to alkaline stress and enhances ABA sensitivity. Full article
Show Figures

Figure 1

16 pages, 1589 KB  
Article
Effects of Rhizopus oligosporus-Mediated Solid-State Fermentation on the Protein Profile and α-Glucosidase Inhibitory Activity of Selenium-Biofortified Soybean Tempeh
by Chengying Wang, Changli Hu, Xin Li, Ruizhe Shen, Liwei Yin, Qiguo Wu and Ting Hu
Foods 2025, 14(16), 2899; https://doi.org/10.3390/foods14162899 - 21 Aug 2025
Viewed by 1221
Abstract
Solid-state fermentation (SSF) enhances the nutritional profile of legumes. This study evaluated Rhizopus oligosporus-mediated SSF for selenium (Se) biofortification in soybean tempeh (a traditional Southeast Asian food), assessing the effects of selenate and selenite (0–60 mg kg−1) on R. oligosporus [...] Read more.
Solid-state fermentation (SSF) enhances the nutritional profile of legumes. This study evaluated Rhizopus oligosporus-mediated SSF for selenium (Se) biofortification in soybean tempeh (a traditional Southeast Asian food), assessing the effects of selenate and selenite (0–60 mg kg−1) on R. oligosporus growth, substrate consumption, mycelium morphology, and Se speciation in tempeh. Selenium supplementation at 18–24 mg kg−1 reduced soybean protein content by 9.4~13.8% relative to the protein content of the Se-free fermented tempeh (control group, 19.85%) and significantly promoted proteolysis. Higher concentrations (48–60 mg kg−1) restored protein levels to control values (19%), indicating concentration-dependent regulation of protein stability. Selenate at 42 mg kg−1 significantly increased the levels of flavor amino acids (e.g., glutamate, aspartate), essential amino acids, and total amino acids in tempeh. In contrast, selenite showed no significant improvement in amino acid content and even reduced non-essential amino acids (e.g., alanine, glycine) at high concentrations (42 mg kg−1). Selenium biofortification converted selenate to selenomethionine (SeMet) and Se(VI), but transformed selenite into methylselenocysteine (MeSeCys), selenocystine (SeCys2), and SeMet. Fermented Se-tempeh demonstrated potent α-glucosidase inhibition (IC50 values ranging from 1.66 ± 0.05 to 2.89 ± 0.03 mg mL−1), suggesting Se-enriched soybean tempeh could be considered a promising blood-sugar-friendly food. Thus, developing soybean-based functional foods via co-inoculation of R. oligosporus with inorganic Se is a promising way to enhance tempeh bioactivity. Full article
(This article belongs to the Section Food Nutrition)
Show Figures

Figure 1

18 pages, 4498 KB  
Article
Changes in Microbial Diversity During Dictyophora indusiata Mycelium Regression Period
by Jie Cheng, Lei Ye, Xin Li, Yunfu Gu, Yi Wang, Zebin Zeng, Xiaoxue Liu, Xiaoling Li and Xiaoping Zhang
Horticulturae 2025, 11(8), 981; https://doi.org/10.3390/horticulturae11080981 - 19 Aug 2025
Viewed by 2684
Abstract
Dictyophora indusiata cultivation is severely impeded by premature hyphal regression. This study elucidates the spatiotemporal dynamics of mycelial regression and associated microbial succession in both substrate and soil matrices across progressive regression stages (CK: normal growth; S1: initial recession; S2: advanced recession; S3: [...] Read more.
Dictyophora indusiata cultivation is severely impeded by premature hyphal regression. This study elucidates the spatiotemporal dynamics of mycelial regression and associated microbial succession in both substrate and soil matrices across progressive regression stages (CK: normal growth; S1: initial recession; S2: advanced recession; S3: complete recession). Microscopic analysis revealed preferential mycelial regression in the substrate, preceding soil regression by 1–2 stages. High-throughput sequencing demonstrated significant fungal community restructuring, characterized by a sharp decline in Phallus abundance (substrate: 99.7% → 7.0%; soil: 78.3% → 5.5%) and concomitant explosive proliferation of Trichoderma (substrate: 0% → 45.2%; soil: 0.1% → 55.3%). Soil fungal communities exhibited a higher richness (Chao1, p < 0.05) and stability, attributed to functional redundancy (e.g., Aspergillus, Conocybe) and physical protection by organic–mineral complexes. Conversely, substrate bacterial diversity dominated, driven by organic matter availability (e.g., the Burkholderia–Caballeronia–Paraburkholderia complex surged to 59%) and optimized porosity. Niche analysis confirmed intensified competition in post-regression soil (niche differentiation) versus substrate niche contraction under Trichoderma dominance. Critically, Trichoderma overgrowing was mechanistically linked to (1) nutrient competition via activated hydrolases (e.g., Chit42) and (2) pathogenic activity (e.g., T. koningii causing rot). We propose ecological control strategies: application of antagonistic Bacillus subtilis (reducing Trichoderma by 63%), substrate C/N ratio modulation via soybean meal amendment, and Sphingomonas–biochar soil remediation. This work provides the first integrated microbial niche model for D. indusiata regression, establishing a foundation for sustainable cultivation. Full article
(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)
Show Figures

Figure 1

24 pages, 560 KB  
Review
Tempeh and Fermentation—Innovative Substrates in a Classical Microbial Process
by Katarzyna Górska, Ewa Pejcz and Joanna Harasym
Appl. Sci. 2025, 15(16), 8888; https://doi.org/10.3390/app15168888 - 12 Aug 2025
Viewed by 3113
Abstract
The growing consumer awareness of functional foods has increased interest in fermented plant-based products with enhanced nutritional and health-promoting properties. This comprehensive narrative literature review examines the potential of diverse raw materials for tempeh production beyond traditional soybeans, analysing their nutritional composition, bioactive [...] Read more.
The growing consumer awareness of functional foods has increased interest in fermented plant-based products with enhanced nutritional and health-promoting properties. This comprehensive narrative literature review examines the potential of diverse raw materials for tempeh production beyond traditional soybeans, analysing their nutritional composition, bioactive compounds, and functional properties. A structured literature search was conducted on peer-reviewed publications up to July 2025, focusing on tempeh fermentation technology, chemical composition, and bioactive compounds from various substrates using recognised analytical methods according to Association of Official Analytical Collaboration (AOAC) standards. The analysis of over 25 different substrates revealed significant opportunities for enhancing tempeh’s nutritional profile through alternative raw materials including legumes, cereals, algae, seeds, and agricultural by-products. Several substrates demonstrated superior nutritional characteristics compared with traditional soybean tempeh, notably tarwi (Lupinus mutabilis) with exceptional protein content ((32–53% dry matter (DM)) and mung bean (Vigna radiata) exhibiting remarkably high polyphenol concentrations (137.53 mg gallic acid equivalents (GAE)/g DM). Fermentation with Rhizopus oligosporus consistently achieved substantial reductions in anti-nutritional factors (64–67% decrease in trypsin inhibitors, up to 65% reduction in phytates) while maintaining consistent antioxidant activities (39–70% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition) across most variants. The diversity of bioactive compounds across different substrates demonstrates potential for developing targeted functional foods with specific health-promoting properties, supporting sustainable food system development through protein source diversification. Full article
Show Figures

Figure 1

17 pages, 2507 KB  
Article
Formula Screening and Optimization of Physical and Chemical Properties for Cultivating Flammulina filiformis Using Soybean Straw as Substrate
by Ruixiang Sun, Jiandong Han, Peng Yang, Shude Yang, Hongyan Xie, Jin Li, Chunyan Huang, Qiang Yao, Qinghua Wang, He Li, Xuerong Han and Zhiyuan Gong
Horticulturae 2025, 11(8), 947; https://doi.org/10.3390/horticulturae11080947 - 11 Aug 2025
Viewed by 567
Abstract
Recently, there has been a growing interest in using agricultural and forestry residues to cultivate Flammulina filiformis. However, there is limited research on cultivating F. filiformis with soybean straw as a substrate. This study systematically optimized the cultivation formula for F. filiformis [...] Read more.
Recently, there has been a growing interest in using agricultural and forestry residues to cultivate Flammulina filiformis. However, there is limited research on cultivating F. filiformis with soybean straw as a substrate. This study systematically optimized the cultivation formula for F. filiformis using soybean straw as the raw substrate and explored the effects of the water content, carbon-to-nitrogen ratio (C/N ratio), substrate particle size, and substrate loading on its growth and development. By replacing corncob, wheat bran, and soybean hulls with soybean straw and increasing the proportion of rice bran, the cultivation formula for growing F. filiformis was optimized. We found that the maximum fruiting body yield of 405 g (330 g dry substrate per bottle) and a biological efficiency of 122.73% were achieved using a substrate mixture of 25% soybean straw, 20% corncob, 20% cottonseed hull, 25% rice bran, 8% wheat bran, 1% CaCO3, and 1% shellfish powder. The yield and biological efficiency of fruiting bodies cultivated on the substrate containing 25% soybean straw did not show significant differences compared to the control group. However, the cultivation formula containing 25% soybean straw yielded F. filiformis with significantly higher levels of amino acids, essential amino acids, and fat. These findings suggest that the 25% soybean straw substrate formulation can serve as a viable alternative to the control formulation for the cultivation of F. filiformis, although variations in the nutritional composition exist. Based on this optimized formula, an optimal biological efficiency can be achieved with a substrate-to-water ratio of 1:1.7, a wet substrate loading amount of 940 g (in a 1250 mL cultivation bottle), and a soybean straw particle size range of 6–8 mm. The optimal C/N ratio for cultivating F. filiformis using soybean straw ranges from 27:1 to 32:1. Additionally, orthogonal experiments revealed that the nitrogen content significantly affected the fruiting body yield, stipe length, and stipe diameter, while the water content mainly affected the pileus diameter, pileus thickness, and number of fruit bodies. Under defined conditions (dry substrate loading volume of 337 g (in a 1250 mL cultivation bottle), a substrate-to-water ratio of 1:1.6, and a C/N ratio of 26:1), the maximum yield and biological efficiency per bottle reached 395 g and 117.21%, respectively. Our findings indicate that the F. filiformis cultivation using soybean straw as the raw substrate exhibits a promising performance and extensive application potential. Full article
(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)
Show Figures

Figure 1

24 pages, 884 KB  
Article
Optimizing Aspergillus oryzae Inoculation Dosage and Fermentation Duration for Enhanced Protein Content in Soybean Meal and Its Influence on Dog Food Extrusion
by Youhan Chen, Thomas Weiss, Donghai Wang, Sajid Alavi and Charles Gregory Aldrich
Processes 2025, 13(8), 2441; https://doi.org/10.3390/pr13082441 - 1 Aug 2025
Viewed by 988
Abstract
This study aimed to optimize the inoculation dosage and fermentation duration to enhance the protein content and reduce soluble oligosaccharides in soybean meal using Aspergillus oryzae and assessed its performance in dog food extrusion. A 3 × 5 factorial design was used to [...] Read more.
This study aimed to optimize the inoculation dosage and fermentation duration to enhance the protein content and reduce soluble oligosaccharides in soybean meal using Aspergillus oryzae and assessed its performance in dog food extrusion. A 3 × 5 factorial design was used to determine the optimal fermentation conditions. These conditions were applied to ferment soybean meal in bulk for nutritional analysis. Finally, the impact of fermentation on extrusion processing was assessed by formulating and extruding four diets: SBM (30% soybean meal), AMF (30% soybean meal with 1% Amaferm®A. oryzae biomass), FSBM (30% fermented soybean meal), and SPI (18% soy protein isolate). Diets were extruded with a single-screw extruder, and physical characteristics of kibbles, particle size distribution, and viscosity of raw mixes were analyzed. The optimal fermentation conditions were 1 × 104 spore/g substrate for 36 h, which increased the crude protein content by 4.63% DM, methionine and cysteine total content by 0.15% DM, and eliminated sucrose, while significantly reducing stachyose, raffinose, and verbascose (95.22, 87.37, and 41.82%, respectively). The extrusion results showed that FSBM had intermediate specific mechanical energy (SME), in-barrel moisture requirements, and sectional expansion index (198.7 kJ/kg, 28.2%, and 1.80, respectively) compared with SBM (83.7 kJ/kg, 34.5%, and 1.30, respectively) and SPI (305.3 kJ/kg, 33.5%, and 2.55, respectively). The FSBM also exhibited intermediate particle size distribution and the least raw mix viscosity. These findings demonstrate that A. oryzae fermentation enhances the nutrient profile of soybean meal while improving extrusion efficiency and kibble quality, supporting its potential use as a sustainable pet food ingredient. Full article
(This article belongs to the Special Issue Feature Papers in the "Food Process Engineering" Section)
Show Figures

Figure 1

27 pages, 4348 KB  
Article
Valorization of Riceberry Broken Rice and Soybean Meal for Optimized Production of Multifunctional Exopolysaccharide by Bacillus tequilensis PS21 with Potent Bioactivities Using Response Surface Methodology
by Thipphiya Karirat, Worachot Saengha, Nantaporn Sutthi, Pheeraya Chottanom, Sirirat Deeseenthum, Nyuk Ling Ma and Vijitra Luang-In
Polymers 2025, 17(15), 2029; https://doi.org/10.3390/polym17152029 - 25 Jul 2025
Viewed by 684
Abstract
This study explores the valorization of agro-industrial by-products—riceberry broken rice (RBR) and soybean meal (SBM)—as cost-effective substrates for enhancing exopolysaccharide (EPS) production by Bacillus tequilensis PS21. Eight Bacillus strains were screened, and B. tequilensis PS21 demonstrated the highest EPS yield (2.54 g/100 mL [...] Read more.
This study explores the valorization of agro-industrial by-products—riceberry broken rice (RBR) and soybean meal (SBM)—as cost-effective substrates for enhancing exopolysaccharide (EPS) production by Bacillus tequilensis PS21. Eight Bacillus strains were screened, and B. tequilensis PS21 demonstrated the highest EPS yield (2.54 g/100 mL DW). The EPS displayed a strong antioxidant capacity with 65.5% DPPH and 80.5% hydroxyl radical scavenging, and a FRAP value of 6.51 mg Fe2+/g DW. Antimicrobial testing showed inhibition zones up to 10.07 mm against Streptococcus agalactiae and 7.83 mm against Staphylococcus aureus. Optimization using central composite design (CCD) and the response surface methodology (RSM) revealed the best production at 5% (w/v) RBR, 3% (w/v) SBM, pH 6.66, and 39.51 °C, yielding 39.82 g/L EPS. This EPS is a moderate-molecular-weight (11,282 Da) homopolysaccharide with glucose monomers. X-ray diffraction (XRD) showed an amorphous pattern, favorable for solubility in biological applications. Thermogravimetric analysis (TGA) demonstrated thermal stability up to ~250 °C, supporting its suitability for high-temperature processing. EPS also exhibited anticancer activity with IC50 values of 226.60 µg/mL (MCF-7) and 224.30 µg/mL (HeLa) at 72 h, reduced colony formation, inhibited cell migration, and demonstrated anti-tyrosinase, anti-collagenase, and anti-elastase effects. This study demonstrates the successful valorization of agro-industrial by-products—RBR and SBM—for the high-yield production of multifunctional EPS with potent antioxidant, antimicrobial, and anticancer properties. The findings highlight the sustainable potential of these low-cost substrates in supporting the development of green and value-added bioproducts, with promising utilizations across the food, pharmaceutical, and cosmetic sectors. Full article
(This article belongs to the Topic Microbes and Their Products for Sustainable Human Life)
Show Figures

Figure 1

25 pages, 3057 KB  
Article
Phylogenetic Diversity and Symbiotic Effectiveness of Bradyrhizobium Strains Nodulating Glycine max in Côte d’Ivoire
by Marie Ange Akaffou, Romain Kouakou Fossou, Anicet Ediman Théodore Ebou, Zaka Ghislaine Claude Kouadjo-Zézé, Chiguié Estelle Raïssa-Emma Amon, Clémence Chaintreuil, Saliou Fall and Adolphe Zézé
Agronomy 2025, 15(7), 1720; https://doi.org/10.3390/agronomy15071720 - 17 Jul 2025
Viewed by 955
Abstract
Soybean (Glycine max) is a protein-rich legume crop that plays an important role in achieving food security. The aim of this study was to isolate soybean-nodulating rhizobia from Côte d’Ivoire soils and evaluate their potential as efficient strains in order to [...] Read more.
Soybean (Glycine max) is a protein-rich legume crop that plays an important role in achieving food security. The aim of this study was to isolate soybean-nodulating rhizobia from Côte d’Ivoire soils and evaluate their potential as efficient strains in order to develop local bioinoculants. For this objective, 38 composite soil samples were collected from Côte d’Ivoire’s five major climatic zones. These soils were used as substrate to trap the nodulating rhizobia using the promiscuous soybean variety R2-231. A total of 110 bacterial strains were isolated and subsequently identified. The analysis of ITS (rDNA16S-23S), glnII and recA sequences revealed a relatively low genetic diversity of these native rhizobia. Moreover, the ITS phylogeny showed that these were scattered into two Bradyrhizobium clades dominated by the B. elkanii supergroup, with ca. 75% of all isolates. Concatenated glnII-recA sequence phylogeny confirmed that the isolates belong in the majority to ‘B. brasilense’, together with B. vignae and some putative genospecies of Bradyrhizobium that needs further elucidation. The core gene phylogeny was found to be incongruent with nodC and nifH phylogenies, probably due to lateral gene transfer influence on the symbiotic genes. The diversity and composition of the Bradyrhizobium species varied significantly among different sampling sites, and the key explanatory variables identified were carbon (C), magnesium (Mg), nitrogen (N), pH, and annual precipitation. Based on both shoot biomass and leaf relative chlorophyll content, three isolates consistently showed a higher symbiotic effectiveness than the exotic inoculant strain Bradyrhizobium IRAT-FA3, demonstrating their potential to serve as indigenous elite strains as bioinoculants. Full article
(This article belongs to the Section Agricultural Biosystem and Biological Engineering)
Show Figures

Figure 1

13 pages, 532 KB  
Article
In Vitro Fermentation Characteristics of Pelagic Sargassum for Inclusion in Integral Diets for Ruminants
by Luis Alberto Canul-Ku, Fernando Casanova-Lugo, Edgar Aguilar-Urquizo, Ingrid Valdivieso-Pérez, Darwin Arcos-Álvarez, Jorge Canul-Solís, Luis Castillo-Sánchez, Alfonso Chay-Canul, Benito Dzib-Castillo and Angel Piñeiro-Vázquez
Fermentation 2025, 11(7), 390; https://doi.org/10.3390/fermentation11070390 - 8 Jul 2025
Viewed by 1674
Abstract
Pelagic sargassum arriving in the Mexican Caribbean is a mixture of brown macroalgae containing polysaccharides, minerals, and secondary metabolites with potential in ruminant diets. The objective of the present study was to evaluate the effect of the inclusion of sargassum in integral diets [...] Read more.
Pelagic sargassum arriving in the Mexican Caribbean is a mixture of brown macroalgae containing polysaccharides, minerals, and secondary metabolites with potential in ruminant diets. The objective of the present study was to evaluate the effect of the inclusion of sargassum in integral diets (ID) on in vitro fermentation characteristics. A completely randomized design was used. The treatments were different levels of sargassum (ICD: 0%, ID10: 10%, ID20: 20% and ID30: 30%) added to a basal substrate (a mixture of Pennisetum purpureum Vc. CT-115 hay, corn, soybean, and molasses). Rumen fluid was obtained from five male lambs with a body weight of 40 ± 3 kg. In vitro gas production (IVGP) as well as dry matter degradability (DMD) and organic matter degradability (DOM) increased linearly (p < 0.0001) as the proportion of sargassum increased at 24, 48, and 72 h. Rumen fluid pH decreased (p < 0.05) with 30% inclusion at 48 h, while protozoan concentration was similar (p > 0.05) in all treatments with respect to the control at all evaluation times. These results indicate that the inclusion of pelagic sargassum in integral concentrated diets improves fermentative parameters, and its inclusion in diets for ruminants is feasible. This opens up a window of opportunity for its study as a novel additive or unconventional supplement. However, in vivo studies are necessary to rule out harmful effects on animal health and performance. Full article
(This article belongs to the Section Industrial Fermentation)
Show Figures

Figure 1

15 pages, 2577 KB  
Article
Adaptive Laboratory Evolution of a Microbial Consortium Enhancing Non-Protein Nitrogen Assimilation for Feed Protein Production
by Yi He, Shilei Wang, Yifan Mi, Mengyu Liu, Huimin Ren, Zhengxiang Guo, Zhen Chen, Yafan Cai, Jingliang Xu, Dong Liu, Chenjie Zhu, Zhi Wang and Hanjie Ying
Microorganisms 2025, 13(6), 1416; https://doi.org/10.3390/microorganisms13061416 - 18 Jun 2025
Viewed by 1062
Abstract
The increasing global demand for protein underscores the necessity for sustainable alternatives to soybean-based animal feed, which poses a challenge to human food security. Thus, the search for sustainable, alternative protein sources is transforming the feed industry in its effort to sustainable operations. [...] Read more.
The increasing global demand for protein underscores the necessity for sustainable alternatives to soybean-based animal feed, which poses a challenge to human food security. Thus, the search for sustainable, alternative protein sources is transforming the feed industry in its effort to sustainable operations. In this study, a microbial consortium was subjected to adaptive laboratory evolution using non-protein nitrogen (NPN) and wheat straw as the sole carbon source. The evolved microbial consortium was subsequently utilized to perform solid-state fermentation on wheat straw and NPN to produce feed protein. After 20 generations, the microbial consortium demonstrated tolerance to 5 g/L NPN, including ammonium sulfate, ammonium chloride, and urea, which represents a fivefold increase compared to the original microbial consortium. Among the three NPNs tested, the evolved microbial consortium exhibited optimal growth performance with ammonium sulfate. Subsequently, the evolved microbial consortium was employed for the solid-state fermentation (SSF) of wheat straw, and the fermentation conditions were optimized. It was found that the true protein content of wheat straw could be increased from 2.74% to 10.42% under specific conditions: ammoniated wheat straw (15% w/w), non-sterilization of the substrate, an inoculation amount of 15% (v/w), nitrogen addition amount of 0.5% (w/w), an initial moisture content of 70%, a fermentation temperature of 30 °C, and a fermentation duration of 10 days. Finally, the SSF process for wheat straw was successfully scaled up from 0.04 to 2.5 kg, resulting in an increased true protein content of 9.84%. This study provides a promising approach for the production of feed protein from straw and NPN through microbial fermentation, addressing protein resource shortages in animal feed and improving the value of waste straw. Full article
(This article belongs to the Special Issue Microbial-Sourced Nutritional Supplements for Human and Animal)
Show Figures

Graphical abstract

Back to TopTop