Search Results (7,067)

In our previous work, we reported for the first time the presence of enterocin-encoding genes in novel Lacticaseibacillus paracasei (L. paracasei) and Levilactobacillus brevis (Lev. brevis) strains isolated from artisanal dairy products made from raw cow milk. The aim of this study was to isolate enterocin-positive lactic acid bacteria (LAB) from artisanal dairy products and assess their technological characteristics and safety for potential application in food systems. LAB isolates were characterized using phenotypic tests, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) identification, and PCR detection of enterocin genes, followed by evaluation of their physiological and technological properties and a comprehensive safety assessment, including antimicrobial resistance, virulence, biogenic amine, and integron genes. Two strains, L. paracasei S2 and Lev. brevis S62, carried enterocin genes (entAS-48 and entQ) and exhibited strong acidifying and proteolytic activities, along with antibacterial effects against foodborne pathogens and reference strains. Both isolates tolerated environmental stresses, including low pH, and lacked virulence factors, clinically relevant antibiotic resistance genes, biogenic amine production, and integron elements. These results indicate that the strains are safe, multifunctional, and suitable for developing regionally adapted dairy products, highlighting artisanal dairy products as a valuable source of novel LAB with promising biotechnological applications. Full article

Rapid urbanization has intensified ecological problems such as landscape fragmentation and biodiversity decline, underscoring the need to maintain regional ecological integrity. The construction of ecological security patterns and the optimization of ecological restoration areas are crucial for addressing these ecological issues. However, research on how to couple ecological security patterns with ecological risk assessment to scientifically identify priority areas for ecological restoration and guide spatially targeted restoration remains insufficient. To address this gap, we investigated Liaoning Province by integrating morphological spatial pattern analysis, landscape connectivity assessment, and ecosystem service hotspot analysis to identify ecological sources. We then applied the minimum cumulative resistance model and circuit theory to extract ecological corridors, constructing a comprehensive ecological security pattern. Integrating landscape ecological risk assessment with ecological security patterns established a conservation and restoration-oriented ecological security framework. The results show that the ecological security pattern comprises 40 ecological source patches and 89 potential ecological corridors. Ecological sources encompass a total of 17,628 km² (approximately 12% of the province), primarily comprising water bodies, grasslands, shrublands, and forests. The ecological corridors span a total of 3533.9 km, with an average length of 39.7 km. We also identified 139 ecological pinch points and 109 ecological barrier points. Integrating these findings with landscape ecological risk zoning delineates ecological restoration zones, revealing a spatial pattern characterized by east–west differentiation and north–south continuity. This ecological conservation and restoration network provides a clear spatial guide and a robust scientific foundation for territorial spatial planning, ecological conservation, and restoration efforts. Full article

Habitat fragmentation due to anthropogenic pressures threats functional connectivity across landscapes for flying mammals. Tadarida brasiliensis depends on nocturnal movement corridors linking refuge and foraging areas, yet these pathways are increasingly constrained in semi-arid regions of northern Mexico. This study developed and analyzed the potential ecological corridors connecting the main colony of T. brasiliensis located in Santa Eulalia with the Irrigation District 005 Delicias, in Chihuahua, Mexico. We integrated multi-source geospatial data within a geographic information system, including wind speed, terrain slope, normalized difference vegetation index, land surface temperature, distance to rivers, landscape aggregation, nighttime lighting, and distance to roads, power lines, and human settlements. Landscape resistance to movement was assessed using a combined framework based on the Analytic Hierarchy Process, the InVEST-Habitat Quality model, and Least Cost Path analysis, generating composite resistance. Five potential corridors were identified, with ranges of lengths and CWD:EucD ratios of 6.8–34.0 km and 20.4–51.3, respectively, reflecting variable cumulative resistance along pathways. Nighttime lighting and proximity to urban areas were major contributors to high resistance, particularly within urban and agricultural environments. The identified corridor network provides a spatial representation of potential routes and supports landscape-level conservation planning to mitigate anthropogenic pressures and maintain functional connectivity. Full article

This study systematically evaluated insect resistance in transgenic poplar lines carrying three distinct Bacillus thuringiensis (Bt) gene vector architectures: a single-gene pb vector (Cry1Ac), a reverse-oriented double-gene n19 vector (Cry1Ac-Cry3A), and a forward-oriented double-gene n5 vector (Cry3A-Cry1Ac). The transgenic lines were accordingly designated as pb8/pb9, n19a/n19b, and DB7/DB16, respectively. Molecular analyses confirmed stable Bt gene integration, with the expression of Cry3A being consistently higher than that of Cry1Ac expression. Bioassays showed that dual-gene lines conferred broader insect resistance to pests than that of single-gene lines against both lepidopteran (Hyphantria cunea) and coleopteran (Plagiodera versicolora, Anoplophora glabripennis) pests. In contrast, the single-gene line pb9 exhibited specialized, high efficacy against H. cunea, achieving 100% mortality. Transcriptomic analysis of P. versicolora larvae fed the double-gene high-resistance n19a line and low-resistance DB16 line revealed multi-level molecular responses to Bt stress, including up-regulation of toxin-activating proteases, altered receptor expression, and suppression of growth-related genes. These changes were associated with significant developmental delay (8.33–20.83% reduction in the molting index). Our findings characterize the insect resistance and molecular profiles of the six transgenic poplar lines, as follows: multi-gene lines (n19a/n19b and DB7/DB16) confer broad-spectrum pest resistance, whereas single-gene lines (pb8/pb9) exhibit targeted efficacy. These results support the utility of these lines for pest-specific poplar breeding programs. Full article

The demand for food is increasing with the rise in the human population. Among foods, meat is an essential part of human nutrition. Meat provides good-quality protein and all the micronutrients needed by humans. In addition, it also contains some bioactive compounds that are good for human health. Increasing demand, together with concerns over food safety, requires new approaches to guarantee a sustainable, safe, and healthy meat supply chain. The only way to get over these challenges is through technological innovations that are capable of enhancing the safety, quality, and sustainability of meat. Herein, this review identifies the need for new methods of rapid microbial detection, biosensors, AI-based monitoring, innovative processing and preservation techniques, precision livestock farming, resource-efficient feed and water management, alternative protein sources, and circular economy approaches. In particular, this review examines some meat analogs like cultured meat, hybrid products, and microbial proteins as environmentally friendly and nutritionally balanced alternatives. These changes in technology can also bring benefits to consumers in terms of their health. The health benefits of these technological innovations for consumers go beyond just safety, including improved nutritional profiles, functional bioactive ingredients, and the prevention of antimicrobial resistance. The review further analyzes policies, regulatory frameworks, and ethical considerations necessary to achieve consumer trust and social acceptance, including the global alignment of standards, certification, labeling, and all issues related to ethics. Furthermore, AI, IoT, Big Data, and nutritional technologies represent new emerging trends able to unleash new opportunities for the optimization of production, quality control, and personalized nutrition. Full article

Wastewater-based surveillance (WBS) is valuable for monitoring antimicrobial resistance (AMR). Staphylococci are key targets, as wastewater can facilitate gene transfer and resistance emergence. Data on WBS for population-level AMR in the Arabian-Gulf remain limited. This study assessed Staphylococcus diversity and resistance in Dubai wastewater. Samples were collected over eight months from nine community sites, two hospital nodes, and two wastewater treatment plants (WWTPs) and were analysed by culture-based method. Ninety-six Staphylococcus isolates were recovered from community, hospital, and WWTP influent, with no growth in effluent. Most isolates (n/N = 88/96) were coagulase-negative Staphylococcus (CoNS), spanning 15 species, dominated by S. saprophyticus, S. cohnii and S. sciuri. The only coagulase-positive Staphylococcus was S. aureus (n = 8) and the only species detected across all wastewater sources. Resistance was highest to benzylpenicillin (88%) and fusidic acid (82%), whereas all isolates remained susceptible to glycopeptides, tigecycline, and linezolid. Fusidic acid resistance was higher in community-wastewater isolates, whereas β-lactam resistance predominated in hospital-wastewater isolates. Sixty percent of CoNS were multidrug-resistant; methicillin resistance occurred in 37.5% of CoNS and 50% of S. aureus. Wastewater is a reservoir of diverse multidrug-resistant staphylococci, underscoring One Health relevance reflecting the potential for circulation between humans, animals, and the shared environment. WBS can support population-level AMR monitoring to inform public health and veterinary interventions. Full article

This study presents the experimental optimization of an interferometric optical fiber sensor for acoustic emission (AE) detection. The system employs a simple and low-cost structure composed of sensing and reference fibers, enabling interference-based detection without specialized components such as fiber Bragg gratings or Fabry–Perot cavities. A narrowband laser source was selected through comparative experiments for its superior stability and interference performance. The influence of fiber-loop parameters, including the number of turns and the optical-path intensity ratio, was systematically evaluated to clarify their effects on AE sensitivity and frequency response. The experimental results demonstrate that detection performance and bandwidth can be flexibly tuned by optimizing the loop configuration. Finally, the sensor was validated using a tensile test, successfully detecting AE signals in the range of 20 kHz to 1 MHz. The proposed system provides a robust, EMI-resistant, and cost-effective interferometric solution for AE monitoring. Full article

Cancer remains one of the leading causes of death worldwide, and resistance to conventional therapies underscores the need for the discovery of novel antitumor agents. The ongoing search for novel natural sources offers promising avenues for discovering unique anticancer compounds with new mechanisms of action. One of these natural sources is represented by fungi, a prolific group of endophytic and non-endophytic eukaryotes able to produce bioactive secondary metabolites, many of which exhibit potent antitumor properties. These natural compounds display diverse chemical structures including polyketides, terpenoids, alkaloids, amino acid-derived compounds, phenols, etc. Their mechanisms of action are equally varied, ranging from induction of apoptosis and cell cycle arrest to inhibition of angiogenesis and metastasis. In this review we describe some potential antitumor compounds of fungal origin, together with the characteristics and biosynthesis of three representative types of antitumor compounds produced by filamentous fungi: squalene-derived sterol-type antitumor agents, prenylated diketopiperazine antitumor metabolites and meroterpenoid antitumor compounds. The ongoing scientific debate regarding the presence of paclitaxel biosynthetic genes in fungi is also discussed. As drug resistance remains a challenge in cancer therapy, fungal compounds offer a valuable reservoir for the development of new chemotherapeutic agents with novel modes of action. Full article

Fresh produce represents a key interface in the One Health continuum, connecting environmental, agricultural and clinical settings where opportunistic bacteria can circulate. Among them, Stenotrophomonas comprises an environmental genus of growing concern due to its multidrug resistance and rising clinical relevance. To investigate their diversity and pathogenic potential, nineteen isolates from vegetables, irrigation water and hospital sources were characterized by MLST, growth kinetics, biofilm formation, antimicrobial susceptibility assays and whole-genome sequencing. Phylogenetic analyses grouped 12 isolates within the Stenotrophomonas maltophilia complex (SMC) (clinical S. maltophilia (n = 7) and environmental S. geniculata (n = 4) and S. sepilia (n = 1)) and seven non-SMC isolates, including S. indicatrix (n = 5) and two unclassified clinical strains. Environmental S. geniculata and S. sepilia isolates showed robust growth at 37 °C and biofilm formation comparable to clinical lineages. Genomic analyses further revealed shared mobile loci (afaD, fhaB, zot) and homologous plasmids between environmental and clinical isolates, suggesting a connected gene pool. The identification of environmental strains with virulence-associated traits and clinical-like phenotypes supports fresh produce as a potential reservoir and transmission route for opportunistic Stenotrophomonas, underscoring the need for integrated surveillance across the food–health interface. Full article

In recent years, the World Health Organization has highlighted biofilm-derived multidrug-resistant bacteria as a critical threat to both global health and the environment. Although various testing methods are available, crystal violet and Congo Red methods are among the most frequently used methods for biofilm detection in the literature. However, inconsistent findings across studies have raised concerns. To address these issues and offer valuable insights for researchers in the field, this study used clinically relevant standard bacterial strains (ATCC or NCTC strains) to perform biofilm assays with both methods and compare the results. To investigate the effect of different sugar sources on biofilm formation, various sugar substrates were also examined using both biofilm methods under controlled culture conditions in this study. When the results were evaluated, significant differences were found between the two methods closely related to sugar content. Of the 22 strains tested, 17 (77%) showed different biofilm results in a sugar-free environment. Similar inconsistencies were also observed with glucose (32% of strains) and sucrose (50% of strains). With fructose, some strains (P. aeruginosa strains, E. faecalis ATCC 29212, K. pneumoniae High Level ESBL, K. pneumoniae BAA 1706, A. baumannii BAA 747) were negative with Congo Red and positive with crystal violet, while others (S. mutans ATCC 25175, E. coli NCTC 13846, E. coli ATCC 25922) gave the opposite results. Fructose caused inconsistencies in approximately 45% of strains. The highest agreement between the two methods (approximately 68%) was observed when glucose was used as the carbon source. Full article

L-shaped tunnels frequently occur in underground coal mines because of geological and operational limitations. Their complex geometry increases ventilation resistance and causes non-uniform airflow, promoting combustible gas accumulation and resulting in a greater fire risk than in straight tunnels. In this work, Fire Dynamics Simulator was employed to quantify the effects of the fire source’s transverse position, curvature radius, heat release rate, and imposed longitudinal ventilation on both the critical velocity and the extent of smoke back-layering. The analysis shows that higher heat-release rates elevate the critical velocity, whereas a centrally located fire yields the lowest value. Shifting the fire toward either sidewall or adopting a larger curvature radius results in a higher critical velocity. In addition, the extent of upstream smoke back-layering increases with curvature, peaking when the ignition point lies close to the convex sidewall. Specifically, with a ventilation velocity of 0.95 m/s and a centerline fire, the back-layering length extends from 23 m ($R$ = 5 m) to 40 m ($R$ = 10 m). Based on theoretical derivation and dimensional analysis, several dimensionless parameters were developed that incorporate both the transverse fire-source position and the curvature radius to modify the dimensionless heat-release rate. Finally, dimensionless predictive models for the critical velocity and back-layering length, incorporating the effects of the curvature radius and the fire transverse position, were developed. These models provide a theoretical foundation and practical framework for fire prevention and ventilation design in L-shaped tunnels. Full article

Rural and agricultural runoff continues to pose a threat to water quality and human health despite a plethora of research identifying likely causes. Large livestock operations and leaking septic systems have proven to be significant sources of both nutrients and bacteria in the form of algal blooms and antibiotic-resistant Escherichia coli. These impacts are often witnessed on a watershed scale. Implementing remedies is complicated, as livestock operations are defined as point-source facilities under the USA Clean Water Act (CWA) but regulated as non-point-source entities under a NPDES CAFO general permit. Non-point-source pollutant assessment of watersheds involves a wide array of sampling parameters that focus primarily on impacts after-the-fact and lack regulatory teeth. This watershed management approach is not sustainable, as evidenced by the continual degradation of our rural watersheds. This study lays out streamlined methods and techniques incorporating focused parameters that can infer point-source pollutant pathways even in already impaired waterways. We applied this methodology to the Pine River Watershed in central Lower Michigan after the appearance of an algal bloom downstream from several potential nutrient inputs. Findings show that the application of these unique methods and techniques results in the successful identification of point-source inputs. These methods are inexpensive and demand few resources, and hence they are easily reproduced and replicated. Therefore, by regulating large livestock operations as point-source discharge entities, it is possible for local communities, educational institutions, and regulatory agencies to identify likely pollutant sources in a way that promotes higher water quality and long-term sustainability. Full article

Highly Pathogenic Avian Influenza (HPAI) viruses, particularly IAV (H5N1), continue to pose a major global threat due to their widespread circulation and high mortality rates in birds. Management of HPAI is complicated by challenges in conserving migratory bird populations, sustaining poultry production, and uncertainties in disease dynamics. Structured decision-making frameworks, such as those based on the PrOACT model, are recommended to improve outbreak response and guide critical actions, especially when HPAI virus (HPAIV) detections occur in sensitive areas like wildlife refuges. Surveillance data from late 2024 to early 2025 show persistent HPAI activity, with 743 detections across 22 European countries and beyond, and notable outbreaks in poultry in nations like Hungary, Iceland, and the UK. The proximity of poultry farms to water sources increases environmental contamination risks. Meanwhile, HPAI A(IAV (H5N1)) and other H5Nx viruses have been detected in a wide range of mammalian species globally, raising concerns about mammalian adaptation due to mutations like E627K and D701N in the PB2 protein. Human infections with IAV (H5N1) have also been reported, with recent cases in North America highlighting zoonotic transmission risks. Molecular studies emphasize the importance of monitoring genetic variations associated with increased virulence and antiviral resistance. Preventive strategies focus on biosafety, personal protective measures, and vaccine development for both avian and human populations. Ongoing genetic characterization and vigilant surveillance remain critical to managing the evolving threat posed by HPAI viruses. Full article

The valorization of grapevine pruning residues through pyrolysis provides a sustainable approach to agricultural waste management, producing biochar with agricultural use potential and carbon sink functionality. This study investigated pruning residues from 12 grapevine cultivars to evaluate the cultivar effects on biochar properties. Samples were collected along the Croatian coast from Istria to Dalmatia and included six indigenous cultivars (Malvazija istarska, Pošip, Maraština, Teran, Plavina, and Plavac mali) and six introduced cultivars (Chardonnay, Pinot blanc, Sauvignon blanc, Merlot, Cabernet Sauvignon, and Syrah). For each cultivar, residues were collected from three distinct vineyards with three replicates per vineyard. Pyrolysis was conducted in a muffle furnace at 400 °C. The pruning residues showed acidic pH (4.79–5.45), moderate electrical conductivity (1694–2390 µS cm⁻¹), and ash contents of 2.65–3.49% among all cultivars. Significant differences were observed among cultivars in residue carbon content and ash fraction, which were reflected in the resulting biochar. Biochar yield ranged from 32% to 35%, while pH values were alkaline, ranging from 10.20 to 11.13. Total carbon increased from 43.77 to 45.36% in grapevine-pruning residues to 65.88–71.57% in biochar. FT-IR spectra revealed cultivar-dependent variation in aromatic C=C intensification, while SEM analysis indicated differences in pore abundance and surface area (1.63–4.13 m² g⁻¹) between cultivars. These results demonstrate that carbon-dense cultivars produced biochars with greater structural stability, indicating enhanced resistance to decomposition. Spectroscopic and microscopic analyses consistently showed increased aromatic condensation, reduced aliphatic functionality, and greater porosity following pyrolysis. These cultivar-dependent differences highlight pruning residues as a chemically heterogeneous but predictable feedstock, with biochar properties primarily governed by the intrinsic characteristics of the source material. Full article

This study investigates the aerodynamic and aeroacoustics behavior of automotive cooling modules in both conventional internal combustion engine (ICE) vehicles and electric vehicles (EVs), with a particular focus on installation effects. Numerical simulations based on the Lattice Boltzmann Method (LBM) are conducted to analyze noise generation mechanisms and flow characteristics across four configurations. The study highlights the challenges of adapting classical cooling module components to EV setups, emphasizing the influence of heat exchanger (HE) placement and duct geometry on noise levels and flow dynamics. The results show that the presence of the HE smooths the upstream flow, improves rotor loading distribution and disrupts long, coherent vortical structures, thereby reducing tonal noise. However, the additional resistance introduced by the HE leads to increased rotor loading and enhanced leakage flow through the shroud-rotor gap. Despite these effects, the overall sound pressure level (OASPL) remains largely unchanged, maintaining a similar magnitude and dipolar directivity pattern as the configuration without the HE. In EV modules, the inclusion of ducts introduces significant flow disturbances and localized pressure fluctuations, leading to regions of high flow rate and rotor loading. These non-uniform flow conditions excite duct modes, resulting in troughs and humps in the acoustic spectrum and potentially causing resonance at the blade-passing frequency, which increases the amplitude in the lower frequency range. Analysis of the loading force components reveals that rotor loading is primarily driven by thrust forces, while duct loading is dominated by lateral forces. Across all configurations, fluctuations at the leading and trailing edges of the rotor are observed, originating from the blade tip and extending to approximately mid-span. These fluctuations are more pronounced in the EV module, identifying it as the dominant source of pressure disturbances. The numerical results are validated against experimental data obtained in the anechoic chamber at the University of Sherbrooke and show good agreement. The relative trends are accurately predicted at lower frequencies, with slight over-prediction, and closely match the experimental data at mid-frequencies. Full article