Research on Biofilm

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Biofilm".

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 1140

Special Issue Editors


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Guest Editor
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain
Interests: device-related infections; biofilm; catheter; prosthesis; prevention; infection; innovation research

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Guest Editor
Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy
Interests: biofilm; infection; inflammation; resistance; antibiotic
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Guest Editor
Department of Microbiology, Clínica Universidad de Navarra, 31008 Pamplona, Spain
Interests: antimicrobials; antibiotics; bacteria; microbial molecular biology

Special Issue Information

Dear Colleagues,

Biofilms are complex communities of microorganisms that adhere to surfaces and tissues and form a protective extracellular matrix. They are particularly problematic because they can resist antibiotics and other antimicrobial agents, as well as the immune system, causing chronic infections, which becomes an important problem for patients and healthcare systems.

Several factors contribute to the formation of bacterial biofilms, including surface properties; host factors, such as immune function and nutritional status; and microbial factors, such as virulence and antibiotic resistance.

Preventing biofilm formation requires strategies that target both microorganisms and hosts. For example, using materials that resist bacterial adhesion or coating devices with antimicrobial agents can help prevent biofilm formation.

The treatment of biofilms is targeted at searching for new strategies acting directly on the affected area or using new antimicrobial agents that can penetrate biofilms.

Research is ongoing to develop new strategies for preventing and treating bacterial and fungal biofilms. In this Special Issue, we encourage researchers and clinicians to submit research articles, review articles, and short communications that address various aspects for a better understanding of biofilm pathogenesis and research on the prevention and treatment of biofilms.

Dr. María Guembe
Dr. Enea G. Di Domenico
Dr. Jose Luis Del Pozo
Guest Editors

Manuscript Submission Information

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Keywords

  • biofilm
  • antimicrobials
  • targeting
  • prevention
  • hosts
  • pathogenesis

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Published Papers (1 paper)

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Research

14 pages, 1049 KB  
Article
Antibiofilm Effects of N-Acetyl Cysteine on Staphylococcal Biofilm in Patients with Chronic Rhinosinusitis
by Ana Jotic, Ivana Cirkovic, Dragana Bozic, Katarina Savic Vujovic, Jovica Milovanovic, Miljan Folic, Aleksandar Trivic, Ljiljana Cvorovic and Nemanja Radivojevic
Microorganisms 2025, 13(9), 2050; https://doi.org/10.3390/microorganisms13092050 - 3 Sep 2025
Viewed by 799
Abstract
Staphylococcal bacterial biofilm plays an important role in the pathogenesis and bacterial persistence of chronic rhinosinusitis. N-acetyl cysteine (NAC) has an inhibitory role in biofilm formation, suppressing adhesion and matrix production or favoring dispersal of preformed biofilm. The aim of this study was [...] Read more.
Staphylococcal bacterial biofilm plays an important role in the pathogenesis and bacterial persistence of chronic rhinosinusitis. N-acetyl cysteine (NAC) has an inhibitory role in biofilm formation, suppressing adhesion and matrix production or favoring dispersal of preformed biofilm. The aim of this study was to examine the in vitro effect of NAC on Staphylococcal biofilm formation by bacterial strains isolated from tissue samples of patients with chronic rhinosinusitis with or without nasal polyps (CRSwNP and CRSsNP). Prospective study included 75 patients with CRS. The biofilm-forming capacity of isolated strains was detected by microtiter-plate method and the effects of sub-inhibitory (1/2x, 1/4x, and 1/8x minimal inhibitory concentration, MIC) and supra-inhibitory minimal concentrations (2x, 4x, and 8xMIC) of NAC on biofilm production were investigated. Staphylococcal bacterial strains were isolated in 54 (72%) patients, and the most frequently isolated species were Staphylococcus aureus (40.7%). Coagulase-negative Staphylococci species were weak producers of biofilm, while S. aureus was a strong biofilm producer. Concentration of 3.1 mg/mL (1/2 MIC) was sufficient to completely prevent biofilm formation in 77.8% of the isolates, where 49.6 mg/mL (8xMIC) led to the complete eradication of formed biofilm in 81.5% of the isolates. The subinhibitory and eradication effects were dose- and strain-dependent. There were no significant differences in MIC values between isolates from patients with CRSwNP and CRSsNP isolates. NAC proved to be effective in inhibiting biofilm formation and reducing formed biofilm by Staphylococcal isolates from patients with CRS. A comparable antibiofilm effect was exhibited in both phenotypes of CRS, indicating that NAC’s antibiofilm activity was independent of the underlying clinical phenotype, and more targeted on biofilm matrix components. Full article
(This article belongs to the Special Issue Research on Biofilm)
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