Search Results (19)

Wetland ecosystems are important habitats for the growth and survival of numerous terrestrial orchids in Europe. This study reviews the current knowledge on the orchids of wetland vegetation in the Central Balkans. The orchid flora was analyzed from taxonomic, phytogeographical, ecological and conservation aspects. The most important taxa include the two Balkan endemics (Dactylorhiza cordigera subsp. bosniaca and D. kalopissi subsp. macedonica) and the three subendemics of the Balkans and the Carpathians (Dactylorhiza cordigera subsp. cordigera, D. maculata subsp. transsilvanica and Gymnadenia frivaldii), as well as a considerable number of Central European, Eurasian and boreal orchid representatives. Several orchid taxa occurring in the wet meadows and fens of the Central Balkans have a southern limit of their distribution in this part of Europe, suggesting that wetlands are important refuges for them. In total, 33 orchid taxa were recorded in plant communities from five classes, 10 orders and 17 alliances. Most orchid taxa grow in the following wetland vegetation types: wet meadows (class Molinio-Arrhenatheretea, order Molinietalia caeruleae, alliances Molinion caeruleae, Deschampsion cespitosae and Calthion palustris); fens (class Scheuchzerio palustris-Caricetea fuscae, order Caricetalia fuscae, alliance Caricion fuscae); tall-herb vegetation along mountain streams and springs (class Mulgedio-Aconitetea); marshes and herb-land vegetation of freshwater or brackish water bodies (class Phragmito-Magnocaricetea). This study highlights the importance of serpentine and silicate wetland vegetation types as important habitats for the survival of terrestrial orchids. In addition, detailed taxonomic, ecological and chorological studies of the wetland orchids of the Central Balkans need to be carried out in order to establish a successful plan for their conservation. Full article

In this study, an electrostatically driven vertical MEMS actuator was designed using a hollow square electrode. To attain vertical actuation, a hollow square-shaped electrode was designed on the glass substrate. The silicon proof mass, containing a step, was utilized to realize analogue actuation without pull-in. The vertical MEMS actuator was fabricated using the SiOG (Silicon on Glass) process and the total actuator size was 8.3 mm × 8.3 mm. The fabricated proof mass was freestanding due to eight serpentine springs with 30 μm width. The vertical movement of the MEMS actuator was successfully controlled electrostatically. The measured vertical movement was 5.6 µm for a voltage of 40 V, applied between the top silicon structure and the hollow square electrode. The results shown here confirm that the proposed MEMS actuator was able to control the vertical displacement using an applied voltage. Full article

Bandgap refers to a frequency band where free waves do not propagate. One of the characteristics of a bandgap is its ability to block the propagation of bending waves in a specific frequency band with a periodic structure. Additionally, it has been reported in previous studies that the vibration-reduction performance of a bandgap is superior to that of other reduction methods. A bandgap can be generated in various frequency bands through a simple parameter change in the unit structure. However, the bandgap for a desired frequency band can be determined accurately only with intensive simulations. To overcome this limitation, we have mathematically derived the bandgap using a serpentine spring as a unit structure. The bandgap equation is derived from the general mass–spring system and the final bandgap is derived by substituting the system into the serpentine resonator. The error map for the major design parameter is confirmed by comparing the derived bandgap with the simulation result. In addition, the theoretical bandgap is compared to the experiment value and the vibration-reduction performance of the serpentine resonator is also confirmed. Based on the theoretical and experimental result, the proposed serpentine resonator verifies that the bandgap can be derived mathematically without numerical analysis. Therefore, serpentine resonator is expected to have various applications since it dramatically reduces the time and cost for forming the bandgap of the desired frequency band. Full article

Audio applications such as mobile phones, hearing aids, true wireless stereo earphones, and Internet of Things devices demand small size, high performance, and reduced cost. Microelectromechanical system (MEMS) capacitive microphones fulfill these requirements with improved reliability and specifications related to sensitivity, signal-to-noise ratio (SNR), distortion, and dynamic range when compared to their electret condenser microphone counterparts. We present the design and modeling of a semiconstrained polysilicon diaphragm with flexible springs that are simply supported under bias voltage with a center and eight peripheral protrusions extending from the backplate. The flexible springs attached to the diaphragm reduce the residual film stress effect more effectively compared to constrained diaphragms. The center and peripheral protrusions from the backplate further increase the effective area, linearity, and sensitivity of the diaphragm when the diaphragm engages with these protrusions under an applied bias voltage. Finite element modeling approaches have been implemented to estimate deflection, compliance, and resonance. We report an 85% increase in the effective area of the diaphragm in this configuration with respect to a constrained diaphragm and a 48% increase with respect to a simply supported diaphragm without the center protrusion. Under the applied bias, the effective area further increases by an additional 15% as compared to the unbiased diaphragm effective area. A lumped element model has been also developed to predict the mechanical and electrical behavior of the microphone. With an applied bias, the microphone has a sensitivity of −38 dB (ref. 1 V/Pa at 1 kHz) and an SNR of 67 dBA measured in a 3.25 mm × 1.9 mm × 0.9 mm package including an analog ASIC. Full article

A dual resonance vibration electromagnetic energy harvester (EMEH) is proposed in this paper to extend frequency range. Compared with the conventional dual resonance harvester, the proposed system realizes an enhanced “band-pass” harvesting characteristic by increasing the relative displacement between magnet and coil among two resonance frequencies with a significant improvement in the average harvested power. Furthermore, two resonant frequencies are decoupled in the proposed system, which leads to a more straightforward design. The proposed dual resonance EMEH is constructed with a tubular dual spring-mass structure. It is designed with a serpentine planar spring and the coil position is optimized for higher power density with an overall size of 53.9 cm³ for the dual resonance EMEH. It realizes an output power of 11 mW at the first resonant frequency of 58 Hz, 14.9 mW at the second resonant frequency of 74.5 Hz, and 0.52 mW at 65 Hz, which is in the middle of the two resonance frequencies. The frequency range of output power above 0.5 mW is from 55.8 Hz to 79.1 Hz. The maximum normalized power density (NPD) reaches up to 2.77 mW/(cm³·g²). Compared with a single resonance harvester design under the same topology and outer dimension at a resonant frequency of 74.5 Hz, the frequency range in the proposed EMEH achieves more than a 2× times extension. The proposed dual resonance EMEH also has more than 2 times wider frequency range than other state-of-art wideband EMEHs. Therefore, the proposed dual resonance EMEH is demonstrated in this paper for a high maximum NPD and higher NPD over a wide frequency range. Full article

(1) Background: The geothermal spring of La Crouen (New Caledonia) discharges warm (42 °C) alkaline water (pH~9) enriched in dissolved nitrogen with traces of methane, but its microbial diversity has not yet been studied. (2) Methods: Cultivation-dependent and -independent methods (e.g., Illumina sequencing and quantitative PCR based on 16S rRNA gene) were used to describe the prokaryotic diversity of this spring. (3) Results: Prokaryotes were mainly represented by Proteobacteria (57% on average), followed by Cyanobacteria, Chlorofexi, and Candidatus Gracilibacteria (GN02/BD1-5) (each > 5%). Both potential aerobes and anaerobes, as well as mesophilic and thermophilic microorganisms, were identified. Some of them had previously been detected in continental hyperalkaline springs found in serpentinizing environments (The Cedars, Samail, Voltri, and Zambales ophiolites). Gammaproteobacteria, Ca. Gracilibacteria and Thermotogae were significantly more abundant in spring water than in sediments. Potential chemolithotrophs mainly included beta- and gammaproteobacterial genera of sulfate-reducers (Ca. Desulfobacillus), methylotrophs (Methyloversatilis), sulfur-oxidizers (Thiofaba, Thiovirga), or hydrogen-oxidizers (Hydrogenophaga). Methanogens (Methanobacteriales and Methanosarcinales) were the dominant Archaea, as found in serpentinization-driven and deep subsurface ecosystems. A novel alkaliphilic hydrogenotrophic methanogen (strain CAN) belonging to the genus Methanobacterium was isolated, suggesting that hydrogenotrophic methanogenesis occurs at La Crouen. Full article

Irrigation with wastewater can strongly contribute to the reduction of water abstraction in agriculture with an especial interest in arid and semiarid areas. However, its use can have drawbacks to both soil and micro-irrigation systems, especially when the total solids in the wastewater are high, such as in digestate liquid fractions (DLF) from plant material. The aim of this study was thus to evaluate the performances of a serpentine shaped micro-emitter injected with a hydrocyclone filtered DLF (HF-DLF) from corn + barley biomass and evaluate the traits of the liquid released within a 8-h irrigation cycle. HF-DLF was injected at 10%, 25%, and 50% dilution compared to tap water (at pH = 7.84) and the system performances were measured. No clogging was found, which likely depended on both the shape of the emitter and the high-pressure head (200 kPa). HF-DLF dilution at 10%, 25%, and 50% consisted in +1.9%, +3.5, and −4.9% amount of liquid released compared to the control. Fluid temperature during irrigation (from 9:00 to 17:00) did not explain the difference in the released amounts of liquid. In 10% HF-DLF % and 25% HF-DLF, a pH difference of + 0.321 ± 0.014 pH units compared to the control was found, and such difference was constant for both dilutions and at increasing the time. In contrast, 50% HF-DLF increased pH by around a half point and such difference increased with time. Similar differences among treatments were found for the total solids in the liquid. These results indicate that 50% HF-DLF was accumulating materials in the serpentine. These results suggest that a low diluted HF-DLF could directly be injected in irrigation systems with few drawbacks for the irrigation system and contribute to water conservation since such wastewater are available from the late spring to the early fall, when water requirements are high. Full article

Even when made by brittle materials, awl-shaped serpentine microsprings (ASSMs) were found to have a nonlinear displacement–force relationship similar to springs made by ductile material. It is found that the nonlinear displacement–force relationship is due to the geometry and dimensions of the ASSMs. The geometric effect of the nonlinear force–displacement relationship of ASSMs for in-plane motion was investigated. A theoretical solution was derived to analyze this nonlinearity. By successfully fabricating and measuring an ASSM, the theoretical results agreed well with the experimental results. The results indicated that ASSMs have a nonlinear force–displacement relationship, which is similar to that of hardening springs. The taper angle has a significant effect on the nonlinear displacement of ASSMs. When the taper angle was small, no obvious effect appeared on the nonlinearity of the microsprings with different numbers of turns. When the beam length increased, the critical force for nonlinear deflection decreased. Full article

In this paper, we report a novel aluminum nitride (AlN) thin-film piezoelectric resonant accelerometer. Different from the ordinary MEMS (micro-electro-mechanical systems) resonant accelerometers, the entire structure of the accelerometer, including the mass and the springs, is excited to resonate in-plane, and the resonance frequency is sensitive to the out-plane acceleration. The structure is centrosymmetrical with serpentine electrodes laid on supporting beams for driving and sensing. The stiffness of the supporting beams changes when an out-plane inertial force is applied on the structure. Therefore, the resonance frequency of the accelerometer will also change under the inertial force. The working principle is analyzed and the properties are simulated in the paper. The proposed AlN accelerometer is fabricated by the MEMS technology, and the structure is released by an ICP isotropic etching. The resonance frequency is 24.66 kHz at a static state. The quality factor is 1868. The relative sensitivity of this accelerometer, defined as the shift in the resonance frequency per gravity unit (1 g = 9.8 m/s²) is 346 ppm/g. The linearity of the accelerometer is 0.9988. The temperature coefficient of frequency (TCF) of this accelerometer is −2.628 Hz/°C (i.e., −106 ppm/°C), tested from −40 °C to 85 °C. Full article

To date, a majority of studies of microbial life in hyperalkaline settings focus on environments that are also highly saline (haloalkaline). Haloalkaline conditions offer microbes abundant workarounds to maintain pH homeostasis, as salt ions can be exchanged for protons by dedicated antiporter proteins. Yet hyperalkaline freshwater systems also occur both naturally and anthropogenically, such as the slag fill aquifers around former Lake Calumet (Chicago, IL, USA). In this study, 16S rRNA gene sequences and metagenomic sequence libraries were collected to assess the taxonomic composition and functional potential of microbes present in these slag-polluted waterways. Relative 16S rRNA gene abundances in Calumet sediment and water samples describe community compositions not significantly divergent from those in nearby circumneutral conditions. Major differences in composition are mainly driven by Proteobacteria, primarily one sequence cluster closely related to Hydrogenophaga, which comprises up to 85% of 16S rRNA gene abundance in hyperalkaline surface sediments. Sequence identity indicates this novel species belongs to the recently established genus Serpentinomonas, a bacterial lineage associated with natural freshwater hyperalkaline serpentinizing springs. Full article

The aim of this work is to present a new georeferenced geological map of an area in the Ligurian Western Alps (Lavagnina Lakes area) that includes both a unique geodiversity and great biodiversity, a peculiar geological heritage, and cultural features. The study area is located in the northern part of the Capanne di Marcarolo Regional Natural Park, occurring in the southern Piedmont Region (Alessandria, NW Italy) and close to the suburbs of Genoa. This area has been studied by multi-disciplinary scientific researchers who, so far, have focused their attention on the occurrence of alkaline springs and investigation of different endemic floral species. Moreover, in the past, the Lavagnina Lakes area has been exploited due to the presence of gold mineralization, and several mining records are still visible. We performed detailed geological mapping at a 1:10,000 scale, and collected data that were later integrated into a digital GIS map. The database associated with the map contains information that may be interesting from different points of view: (i) scientific research; (ii) outreach and dissemination activities; and (iii) geotourism (i.e., trail networks and panoramic viewpoints). The area represents a section of the Jurassic Piedmont Ligurian oceanic lithosphere, showing several geologic processes on different scales, such as the serpentinization process and intense and widespread carbonation of ultramafic rocks; the area is, moreover, characterized by fault systems showing paleoseismic structures. Beyond scientific research activities (i.e., geology, geoarchaeology, and mining archaeology), the area can also be promoted for geotourism, outreach and dissemination activities, field trips for schools, and gold panning activities. Hence, our new digital map and our 3D model could be a useful tool to illustrate the main characteristics of the area, leading a non-expert public to explore different geological features in a relatively “small” area. In this way, our map could help to improve geotourism, be used as a tool for educational activities, and, finally, could also help the Capanne di Marcarolo Regional Natural Park to be recognized as a geopark. Full article

Abiotic methane is widely documented in seeps, springs and aquifers associated with mafic-ultramafic rocks in Phanerozoic ophiolites, peridotite massifs and intrusions worldwide. Chromitites in ophiolites, in particular, have been interpreted as the rocks potentially generating methane though CO₂ hydrogenation. Here, we document, for the first time, the presence of methane within chromitites in South America. We analyzed, through milling and gas extraction, the content of gas occluded in Cedrolina chromitite samples, belonging to the Pilar de Goiás greenstone belt in Brazil. The chromitites display significant gas concentrations up to 0.31 µg CH₄/g_rock and 2800 ppmv of hydrogen, while the host talc schist is devoid of gas. Stable C isotope composition of methane (δ¹³C from −30 to −39.2‰) and the absence of organic-matter rich metasediments in the region suggest an abiotic origin. Hydrogen and methane concentrations appear related to high-Cr chromite modal content and to the presence of Ni-sulfides/alloys, which are potential catalysts of CO₂ hydrogenation at temperatures above 200 °C. Accessory ruthenium-bearing minerals occurring in the chromitites could also act as catalysts, even at lower temperatures. Geothermometry of chlorite found in the chromitites constrains serpentinization at ~250 °C, during lower greenschist facies retrometamorphism. Hydrogen could be autochthonous, and thus formed under similar temperature, which we hypothesize represents the upper limit for abiotic methane generation in the area (250 °C). The Cedrolina chromitites are the first example of CH₄ occurrence in ultramafic rocks related to an Archean-Paleoproterozoic greenstone belt. This may imply that serpentinized Cr-rich chromitites could have been sources of methane for the early Earth’s atmosphere. Full article

Our previous report based on a 10 g (gravity) silicon-based inertial micro-switch showed that the contact effect between the two electrodes can be improved by squeeze-film damping. As an extended study toward its potential applications, the switch with a large proof mass suspended by four flexible serpentine springs was redesigned to achieve 5 g threshold value and enhanced threshold accuracy. The impact of the squeeze-film damping on the threshold value was theoretically studied. The theoretical results show that the threshold variation from the designed value due to fabrication errors can be reduced by optimizing the device thickness (the thickness of the proof mass and springs) and then establishing a tradeoff between the damping and elastic forces, thus improving the threshold accuracy. The design strategy was verified by FEM (finite-element-method) simulation and an experimental test. The simulation results show that the maximum threshold deviation was only 0.15 g, when the device thickness variation range was 16–24 μm, which is an adequately wide latitude for the current bulk silicon micromachining technology. The measured threshold values were 4.9–5.8 g and the device thicknesses were 18.2–22.5 μm, agreeing well with the simulation results. The measured contact time was 50 μs which is also in good agreement with our previous work. Full article

Korenaga and coworkers presented evidence to suggest that the Earth’s mantle was dry and water filled the ocean to twice its present volume 4.3 billion years ago. Carbon dioxide was constantly exhaled during the mafic to ultramafic volcanic activity associated with magmatic plumes that produced the thick, dense, and relatively stable oceanic crust. In that setting, two distinct and major types of sub-marine hydrothermal vents were active: ~400 °C acidic springs, whose effluents bore vast quantities of iron into the ocean, and ~120 °C, highly alkaline, and reduced vents exhaling from the cooler, serpentinizing crust some distance from the heads of the plumes. When encountering the alkaline effluents, the iron from the plume head vents precipitated out, forming mounds likely surrounded by voluminous exhalative deposits similar to the banded iron formations known from the Archean. These mounds and the surrounding sediments, comprised micro or nano-crysts of the variable valence Fe^II/Fe^III oxyhydroxide known as green rust. The precipitation of green rust, along with subsidiary iron sulfides and minor concentrations of nickel, cobalt, and molybdenum in the environment at the alkaline springs, may have established both the key bio-syntonic disequilibria and the means to properly make use of them—the elements needed to effect the essential inanimate-to-animate transitions that launched life. Specifically, in the submarine alkaline vent model for the emergence of life, it is first suggested that the redox-flexible green rust micro- and nano-crysts spontaneously precipitated to form barriers to the complete mixing of carbonic ocean and alkaline hydrothermal fluids. These barriers created and maintained steep ionic disequilibria. Second, the hydrous interlayers of green rust acted as engines that were powered by those ionic disequilibria and drove essential endergonic reactions. There, aided by sulfides and trace elements acting as catalytic promoters and electron transfer agents, nitrate could be reduced to ammonia and carbon dioxide to formate, while methane may have been oxidized to methyl and formyl groups. Acetate and higher carboxylic acids could then have been produced from these C1 molecules and aminated to amino acids, and thence oligomerized to offer peptide nests to phosphate and iron sulfides, and secreted to form primitive amyloid-bounded structures, leading conceivably to protocells. Full article

Native iron has been identified in an active thermogenic travertine deposit, located at Ilia area (Euboea Island, Greece). The deposit is forming around a hot spring, which is part of a large active metallogenetic hydrothermal system depositing ore-bearing travertines. The native iron occurs in two shapes: nodules with diameter 0.4 and 0.45 cm, and angular grains with length up to tens of μm. The travertine laminae around the spherical/ovoid nodules grow smoothly, and the angular grains are trapped inside the pores of the travertine. Their mineral-chemistry is ultra-pure, containing, other than Fe, only Mn (0.34–0.38 wt.%) and Ni (≤0.05 wt.%). After evaluating all the possible environments where native iron has been reported up until today and taking under consideration all the available data concerning the study area, we propose two possible scenarios: (i) Ilia’s native iron has a magmatic/hydrothermal origin i.e., it is a deep product near the magmatic chamber or a peripheral cooling igneous body that was transferred during the early stages of the geothermal field evolution, from high temperature, reduced gas-rich fluids and deposited along with other metals in permeable structural zones, at shallow levels. Later on, it was remobilized and mechanically transferred and precipitated at the Ilia’s thermogenic travertine by the active lower temperatures geothermal fluids; (ii) the native iron at Ilia is remobilized from deep seated ophiolitic rocks, originated initially from reduced fluids during serpentinization processes; however, its mechanical transport seems less probable. The native iron mineral-chemistry, morphology and the presence of the other mineral phases in the same thermogenic travertine support both hypotheses. Full article