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28 pages, 8519 KiB  
Article
Evaluating the Microclimatic Performance of Elevated Open Spaces for Outdoor Thermal Comfort in Cold Climate Zones
by Xuan Ma, Qian Luo, Fangxi Yan, Yibo Lei, Yuyang Lu, Haoyang Chen, Yuhuan Yang, Han Feng, Mengyuan Zhou, Hua Ding and Jingyuan Zhao
Buildings 2025, 15(15), 2777; https://doi.org/10.3390/buildings15152777 - 6 Aug 2025
Abstract
Improving outdoor thermal comfort is a critical objective in urban design, particularly in densely built urban environments. Elevated semi-open spaces—outdoor areas located beneath raised building structures—have been recognized for enhancing pedestrian comfort by improving airflow and shading. However, previous studies primarily focused on [...] Read more.
Improving outdoor thermal comfort is a critical objective in urban design, particularly in densely built urban environments. Elevated semi-open spaces—outdoor areas located beneath raised building structures—have been recognized for enhancing pedestrian comfort by improving airflow and shading. However, previous studies primarily focused on warm or temperate climates, leaving a significant research gap regarding their thermal performance in cold climate zones characterized by extreme seasonal variations. Specifically, few studies have investigated how these spaces perform under conditions typical of northern Chinese cities like Xi’an, which is explicitly classified within the Cold Climate Zone according to China’s national standard GB 50176-2016 and experiences both severe summer heat and cold winter conditions. To address this gap, we conducted field measurements and numerical simulations using the ENVI-met model (v5.0) to systematically evaluate the microclimatic performance of elevated ground-floor spaces in Xi’an. Key microclimatic parameters—including air temperature, mean radiant temperature, relative humidity, and wind velocity—were assessed during representative summer and winter conditions. Our findings indicate that the height of the elevated structure significantly affects outdoor thermal comfort, identifying an optimal elevated height range of 3.6–4.3 m to effectively balance summer cooling and winter sheltering needs. These results provide valuable design guidance for architects and planners aiming to enhance outdoor thermal environments in cold climate regions facing distinct seasonal extremes. Full article
17 pages, 287 KiB  
Article
Nutritional Quality and Safety of Windowpane Oyster Placuna placenta from Samal, Bataan, Philippines
by Jessica M. Rustia, Judith P. Antonino, Ravelina R. Velasco, Edwin A. Yates and David G. Fernig
Fishes 2025, 10(8), 385; https://doi.org/10.3390/fishes10080385 - 6 Aug 2025
Abstract
The windowpane oyster (Placuna placenta) is common in coastal areas of the Philippines, thriving in brackish waters. Its shells underpin the local craft industries. While its meat is edible, only small amounts are consumed locally, most going to waste. Utilization of [...] Read more.
The windowpane oyster (Placuna placenta) is common in coastal areas of the Philippines, thriving in brackish waters. Its shells underpin the local craft industries. While its meat is edible, only small amounts are consumed locally, most going to waste. Utilization of this potential nutrient source is hindered by the lack of information concerning its organic and mineral content, the possible presence of heavy metal ions, and the risk of microbial pathogens. We report extensive analysis of the meat from Placuna placenta, harvested during three different seasons to account for potential variations. This comprises proximate analysis, mineral, antioxidant, and microbial analyses. While considerable seasonal variation was observed, the windowpane oyster was found to be a rich source of protein, fats, minerals, and carbohydrates, comparing well with the meats of other shellfish and land animals. Following pre-cooking (~90 °C, 25–30 min), the standard local method for food preparation, no viable E. coli or Salmonella sp. were detected. Mineral content was broadly similar to that reported in fish, although iron, zinc, and copper were more highly represented, nevertheless, heavy metals were below internationally acceptable levels, with the exception of one of three samples, which was slightly above the only current standard, FSANZ. Whether the arsenic was in the safer organic form, which is commonly the case for shellfish, or the more toxic inorganic form remains to be established. This and the variation of arsenic over time will need to be considered when developing food products. Overall, the meat of the windowpane oyster is a valuable food resource and its current (albeit low-level) use should lower any barriers to its acceptance, making it suitable for commercialization. The present data support its development for high-value food products in urban markets. Full article
(This article belongs to the Section Processing and Comprehensive Utilization of Fishery Products)
17 pages, 1802 KiB  
Article
Longitudinal Profiling of the Human Milk Microbiome from Birth to 12 Months Reveals Overall Stability and Selective Taxa-Level Variation
by Ruomei Xu, Zoya Gridneva, Matthew S. Payne, Mark P. Nicol, Ali S. Cheema, Donna T. Geddes and Lisa F. Stinson
Microorganisms 2025, 13(8), 1830; https://doi.org/10.3390/microorganisms13081830 - 5 Aug 2025
Abstract
Human milk bacteria contribute to gut microbiome establishment in breastfed infants. Although breastfeeding is recommended throughout infancy, temporal variation in the milk microbiome—particularly beyond solid food introduction—remains understudied. We analyzed 539 milk samples from 83 mother–infant dyads between 1 week and 12 months [...] Read more.
Human milk bacteria contribute to gut microbiome establishment in breastfed infants. Although breastfeeding is recommended throughout infancy, temporal variation in the milk microbiome—particularly beyond solid food introduction—remains understudied. We analyzed 539 milk samples from 83 mother–infant dyads between 1 week and 12 months postpartum using full-length 16S rRNA gene sequencing. The microbiota was dominated by Streptococcus (34%), Cutibacterium (12%), and Staphylococcus (9%), with marked inter-individual variation. Microbiome profiles remained largely stable across lactation, with only six taxa showing temporal fluctuations, including increases in typical oral bacteria such as Streptococcus salivarius, Streptococcus lactarius, Rothia mucilaginosa, and Granulicatella adiacens. Richness and evenness were higher at 1 week compared to 1 month postpartum (p = 0.00003 and p = 0.007, respectively), then stabilized. Beta diversity also remained stable over time. Maternal pre-pregnancy BMI was positively associated with Gemella haemolysans (p = 0.016), while Haemophilus parainfluenzae was more abundant in milk from mothers with allergies (p = 0.003) and those who gave birth in autumn or winter (p = 0.006). The introduction of solid food was linked to minor taxonomic shifts. Overall, the milk microbiome remained robustly stable over the first year of lactation, with limited but notable fluctuations in specific taxa. This study supports the role of human milk as a consistent microbial source for infants and identifies maternal BMI, allergy status, and birth season as key variables warranting further investigation. Full article
(This article belongs to the Section Microbiomes)
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19 pages, 1053 KiB  
Article
Evaluating Emissions from Select Urban Parking Garages in Cincinnati, OH, Using Portable Sensors and Their Potentials for Sustainability Improvement
by Alyssa Yerkeson and Mingming Lu
Sustainability 2025, 17(15), 7108; https://doi.org/10.3390/su17157108 - 5 Aug 2025
Abstract
Urban parking around the world faces similar challenges of inadequate space, pollution, and carbon emissions. Although various smart parking technologies have been tested and implemented, they primarily aim to reduce the time spent searching for parking, without considering the impact on air quality. [...] Read more.
Urban parking around the world faces similar challenges of inadequate space, pollution, and carbon emissions. Although various smart parking technologies have been tested and implemented, they primarily aim to reduce the time spent searching for parking, without considering the impact on air quality. In this study, the air quality in three urban garages was investigated with portable instruments at the entrance and exit gates and inside the garages. Garage emissions measured include CO2, PM2.5, PM10, NO2, and total VOCs. The results suggested that the PM2.5 levels in these garages tend to be higher than the ambient levels. The emissions also exhibit seasonal variations, with the highest concentrations occurring in the summer, which are 20.32 µg/m3 in Campus Green, 14.25 µg/m3 in CCM, and 15.23 µg/m3 in Washington Park garages, respectively. PM2.5 measured from these garages is strongly correlated (with an R2 of 0.64) with ambient levels. CO2 emissions are higher than ambient levels but within the indoor air quality limit. This suggests that urban garages in Cincinnati tend to enrich ambient air concentrations, which can affect garage users and garage attendants. Portable sensors are capable of long-term emission monitoring and are compatible with other technologies in smart garage development. With portable air sensors becoming increasingly accessible and affordable, there is an opportunity to integrate these devices with smart garage management systems to enhance the sustainability of parking garages. Full article
(This article belongs to the Special Issue Control of Traffic-Related Emissions to Improve Air Quality)
20 pages, 5212 KiB  
Article
Assessing the Land Surface Temperature Trend of Lake Drūkšiai’s Coastline
by Jūratė Sužiedelytė Visockienė, Eglė Tumelienė and Rosita Birvydienė
Land 2025, 14(8), 1598; https://doi.org/10.3390/land14081598 - 5 Aug 2025
Abstract
This study investigates long-term land surface temperature (LST) trends along the shoreline of Lake Drūkšiai, a transboundary lake in eastern Lithuania that formerly served as a cooling reservoir for the Ignalina Nuclear Power Plant (INPP). Although the INPP was decommissioned in 2009, its [...] Read more.
This study investigates long-term land surface temperature (LST) trends along the shoreline of Lake Drūkšiai, a transboundary lake in eastern Lithuania that formerly served as a cooling reservoir for the Ignalina Nuclear Power Plant (INPP). Although the INPP was decommissioned in 2009, its legacy continues to influence the lake’s thermal regime. Using Landsat 8 thermal infrared imagery and NDVI-based methods, we analysed spatial and temporal LST variations from 2013 to 2024. The results indicate persistent temperature anomalies and elevated LST values, particularly in zones previously affected by thermal discharges. The years 2020 and 2024 exhibited the highest average LST values; some years (e.g., 2018) showed lower readings due to localised environmental factors such as river inflow and seasonal variability. Despite a slight stabilisation observed in 2024, temperatures remain higher than those recorded in 2013, suggesting that pre-industrial thermal conditions have not yet been restored. These findings underscore the long-term environmental impacts of industrial activity and highlight the importance of satellite-based monitoring for the sustainable management of land, water resources, and coastal zones. Full article
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19 pages, 1551 KiB  
Article
Genome-Wide Association Study Reveals Key Genetic Loci Controlling Oil Content in Soybean Seeds
by Xueyang Wang, Min Zhang, Fuxin Li, Xiulin Liu, Chunlei Zhang, Fengyi Zhang, Kezhen Zhao, Rongqiang Yuan, Sobhi F. Lamlom, Honglei Ren, Hongmei Qiu and Bixian Zhang
Agronomy 2025, 15(8), 1889; https://doi.org/10.3390/agronomy15081889 - 5 Aug 2025
Abstract
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean [...] Read more.
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean accessions, primarily sourced from Northeast China, was assessed for seed oil content at Heilongjiang Province in three replications over two growing seasons (2021 and 2023) and underwent genotyping via whole-genome resequencing, resulting in 1,048,576 high-quality SNP markers. Phenotypic analysis indicated notable variation in oil content, ranging from 11.00% to 21.77%, with an average increase of 1.73% to 2.28% across all growing regions between 2021 and 2023. A genome-wide association study (GWAS) analysis revealed 119 significant single-nucleotide polymorphism (SNP) loci associated with oil content, with a prominent cluster of 77 SNPs located on chromosome 8. Candidate gene analysis identified four key genes potentially implicated in oil content regulation, selected based on proximity to significant SNPs (≤10 kb) and functional annotation related to lipid metabolism and signal transduction. Notably, Glyma.08G123500, encoding a receptor-like kinase involved in signal transduction, contained multiple significant SNPs with PROVEAN scores ranging from deleterious (−1.633) to neutral (0.933), indicating complex functional impacts on protein function. Additional candidate genes include Glyma.08G110000 (hydroxycinnamoyl-CoA transferase), Glyma.08G117400 (PPR repeat protein), and Glyma.08G117600 (WD40 repeat protein), each showing distinct expression patterns and functional roles. Some SNP clusters were associated with increased oil content, while others correlated with decreased oil content, indicating complex genetic regulation of this trait. The findings provide molecular markers with potential for marker-assisted selection (MAS) in breeding programs aimed at increasing soybean oil content and enhancing our understanding of the genetic architecture governing this critical agricultural trait. Full article
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22 pages, 5033 KiB  
Article
Seasonal Variation of Air Purifier Effectiveness and Natural Ventilation Behavior: Implications for Sustainable Indoor Air Quality in London Nurseries
by Shuo Zhang, Didong Chen and Xiangyu Li
Sustainability 2025, 17(15), 7093; https://doi.org/10.3390/su17157093 - 5 Aug 2025
Abstract
This study investigates the seasonal effectiveness of high-efficiency particulate air (HEPA) purifiers and window-opening behaviors in three London nurseries, using continuous indoor and outdoor PM2.5 monitoring, window state and air purifier use, and occupant questionnaire data collected from March 2021 to February [...] Read more.
This study investigates the seasonal effectiveness of high-efficiency particulate air (HEPA) purifiers and window-opening behaviors in three London nurseries, using continuous indoor and outdoor PM2.5 monitoring, window state and air purifier use, and occupant questionnaire data collected from March 2021 to February 2022. Of the approximately 40–50 nurseries contacted, only three agreed to participate. Results show that HEPA purifiers substantially reduced indoor particulate matter (PM2.5), with the greatest effect observed during the heating season when windows remained closed for longer periods. Seasonal and behavioral analysis indicated more frequent and longer window opening in the non-heating season (windows were open 41.5% of the time on average, compared to 34.2% during the heating season) driven by both ventilation needs and heightened COVID-19 concerns. Predictive modeling identified indoor temperature as the main driver of window opening, while carbon dioxide (CO2) had a limited effect. In addition, window opening often increased indoor PM2.5 under prevailing outdoor air quality conditions, with mean concentrations rising from 2.73 µg/m3 (closed) to 3.45 µg/m3 (open), thus reducing the apparent benefit of air purifiers. These findings underscore the complex interplay between mechanical purification and occupant-controlled ventilation, highlighting the need to adapt indoor air quality (IAQ) strategies to both seasonal and behavioral factors in educational settings. Full article
(This article belongs to the Special Issue Sustainability and Indoor Environmental Quality)
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18 pages, 3140 KiB  
Article
Spatial and Temporal Distribution of Conversational and Emerging Pollutants in Fecal Sludge from Rural Toilets, China
by Lin Lin, Yilin Shen, Guoji Ding, Shakib Alghashm, Seinn Lei Aye and Xiaowei Li
Sustainability 2025, 17(15), 7088; https://doi.org/10.3390/su17157088 - 5 Aug 2025
Abstract
Effective management of fecal pollutants in rural sanitation is crucial for environmental health and public safety, especially in developing regions. In this study, temporal and regional variations in nutrient elements, heavy metals, pathogenic microorganisms (PMs), and antibiotic resistance genes (ARGs) of fecal samples [...] Read more.
Effective management of fecal pollutants in rural sanitation is crucial for environmental health and public safety, especially in developing regions. In this study, temporal and regional variations in nutrient elements, heavy metals, pathogenic microorganisms (PMs), and antibiotic resistance genes (ARGs) of fecal samples from rural toilets in China were investigated. The moisture contents of the fecal samples average 92.7%, decreasing seasonally from 97.4% in summer to 90.6% in winter. The samples’ pH values range from 6.5 to 7.5, with a slight decrease in winter (6.8), while their electrical conductivity varies from 128.1 to 2150 μs/cm, influenced by regional diets. Chromium (9.0–49.7 mg/kg) and copper (31.9–784.4 mg/kg) levels vary regionally, with higher concentrations in Anhui and Guangxi Provinces due to dietary and industrial factors. Zinc contents range from 108.5 to 1648.9 mg/kg, with higher levels in autumn and winter, resulting from agricultural practices and Zn-containing fungicides, posing potential health and phytotoxicity risks. Seasonal and regional variations in PMs and ARGs were observed. Guangxi Province shows the high PM diversity in summer samples, while Jiangsu Province exhibits the high ARGs types in autumn samples. These findings highlight the need for improved waste management and sanitation solutions in rural areas to mitigate environmental risks and protect public health. Continued research in these regions is essential to inform effective sanitation strategies. Full article
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23 pages, 2733 KiB  
Article
Estimating Genetic Variability and Heritability of Morpho-Agronomic Traits of M5 Cowpea (Vigna unguiculata (L.) Walp) Mutant Lines
by Kelebonye Ramolekwa, Motlalepula Pholo-Tait, Travis Parker, Goitseone Malambane, Samodimo Ngwako and Lekgari Lekgari
Int. J. Mol. Sci. 2025, 26(15), 7543; https://doi.org/10.3390/ijms26157543 - 5 Aug 2025
Abstract
Induced mutation plays an integral part in plant breeding as it introduces new variability among the population. A study was conducted in cowpeas [Vigna unguiculata (L.) Walp] to assess the yield divergence, heritability, genetic advance, and correlation among the M5 Tswana cowpea [...] Read more.
Induced mutation plays an integral part in plant breeding as it introduces new variability among the population. A study was conducted in cowpeas [Vigna unguiculata (L.) Walp] to assess the yield divergence, heritability, genetic advance, and correlation among the M5 Tswana cowpea mutants. The experiment utilized seven genotypes under rainfed and supplementary irrigation during the 2022/23 and 2023/24 cropping seasons. The mutant lines demonstrated significant variations in days to 50% emergence (DE) and days to 50% flowering (DF). Tswana emerged earlier (5–7 days) and flowered in 21–54 days across the two seasons, compared to some of the mutant lines. The yield and yield components varied among some mutant lines and the control. Most importantly, mutants outperformed the Tswana control for some of these traits, indicating potential for genetic improvement. An analysis of genetic parameters revealed minimal environmental influences on some of the observed traits (GH, PN, GY), while others showed little environmental impact. Variation in heritability (H2) and genetic advance (GA%) between the two seasons limited the contribution of genotypic effects in the expression of the studied traits. Correlation analysis revealed strong and significant positive associations between DE and GH, as well as between DF and PW. Most traits, except DF and PW, were positively correlated with grain yield (GY), although the correlations were not significantly different. Cluster analysis grouped the genotypes into four distinct clusters. Principal component analysis (PCA) revealed the superiority of mutant lines (Tswana-300Gy-214, Tswana-400Gy mutant lines, and Tswana-500Gy-31) in their association with improved GY, pod weight (PW), 100-seed weight (100-SW), and seed number per pod (SN/P). Interestingly, the Tswana control formed a separate cluster and diverged from the mutants in PCA, suggesting that induced mutagenesis effectively targeted genes controlling the traits considered in this study. Full article
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16 pages, 3138 KiB  
Article
Seasonal and Interannual Variations (2019–2023) in the Zooplankton Community and Its Size Composition in Funka Bay, Southwestern Hokkaido
by Haochen Zhang, Atsushi Ooki, Tetsuya Takatsu and Atsushi Yamaguchi
Oceans 2025, 6(3), 49; https://doi.org/10.3390/oceans6030049 - 4 Aug 2025
Abstract
Funka Bay, located in southwest Hokkaido, is a vital fishing area with a shallow depth of less than 100 m. Seasonal flows of the Oyashio and Tsugaru Warm Current affect the marine environment, leading to significant changes in zooplankton communities, yet limited information [...] Read more.
Funka Bay, located in southwest Hokkaido, is a vital fishing area with a shallow depth of less than 100 m. Seasonal flows of the Oyashio and Tsugaru Warm Current affect the marine environment, leading to significant changes in zooplankton communities, yet limited information is available on these variations. This study used ZooScan imaging to analyze seasonal and interannual changes in zooplankton abundance, biovolume, community structure, and size composition from 2019 to 2023. Water temperature was low in March–April and high in September–November, with chlorophyll a peaks occurring from February to April. Notable taxa such as Thaliacea, Noctiluca, and cladocerans were more common in the latter half of the year. Interannual variations included a decline in large cold-water copepods, Eucalanus bungii and Neocalanus spp., which were abundant in 2019 but decreased by 2023. Zooplankton abundance and biovolume showed synchronized seasonal changes, correlating with shifts in the Normalized Biovolume Size Spectra (NBSS) index, which measures size composition. Cluster analysis identified eight zooplankton communities, with Community A dominant from July to December across all years, while Community D was prevalent in early 2019 but was replaced in subsequent years. Community E emerged from March to April in 2021–2023. In 2019, large cold-water copepods were dominant, but from 2020 to 2023, appendicularians became the dominant group during the March–April period. The decline in large copepods is likely linked to marine heat waves, influencing yearly zooplankton community changes. Full article
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23 pages, 2353 KiB  
Article
Seaweeds of the Israeli Mediterranean Sea: Nutritional and Biotechnological Potential Through Seasonal and Species Variation
by Doron Yehoshua Ashkenazi, Félix L. Figueroa, Julia Vega, Shoshana Ben-Valid, Guy Paz, Eitan Salomon, Avigdor Abelson and Álvaro Israel
Mar. Drugs 2025, 23(8), 320; https://doi.org/10.3390/md23080320 - 4 Aug 2025
Abstract
Macroalgae (seaweeds) produce unique bioactive metabolites that have enabled their survival for millions of years, offering significant potential for human benefits. In the Israeli Mediterranean Sea, no comprehensive systematic surveys of seaweeds have been published since the 1990s, and their chemical composition remains [...] Read more.
Macroalgae (seaweeds) produce unique bioactive metabolites that have enabled their survival for millions of years, offering significant potential for human benefits. In the Israeli Mediterranean Sea, no comprehensive systematic surveys of seaweeds have been published since the 1990s, and their chemical composition remains largely unexplored. This study presents an extensive survey of intertidal seaweed communities along the shallow Israeli coastline, documenting their spatial, temporal, and biochemical diversity. Of the 320 specimens collected, 55 seaweed species were identified: 29 red (Rhodophyta), 14 brown (Phaeophyceae), and 12 green (Chlorophyta). A significant shift in species abundance was documented, with a single dominant annual bloom occurring during spring, unlike previously reported biannual blooms. Chemical analysis of the dominant species revealed significant seasonal variations in compound levels, with higher protein content in winter and increased antioxidant capacity during spring. Phenolic and natural sunscreen compounds (mycosporine-like amino acids, MAAs) showed no general seasonal trend. These findings highlight the optimal environmental conditions for seaweed growth and underscore their potential for aquaculture and biotechnology. We hypothesize that the ecologically unique conditions of the Israeli Mediterranean Sea may foster resilient seaweed species enriched with distinctive chemical properties, suitable for nutritional, health, pharmaceutical, and nutraceutical applications, particularly as climate-adaptive bioresources. Full article
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16 pages, 3766 KiB  
Article
Evaluation of Energy and CO2 Reduction Through Envelope Retrofitting: A Case Study of a Public Building in South Korea Conducted Using Utility Billing Data
by Hansol Lee and Gyeong-Seok Choi
Energies 2025, 18(15), 4129; https://doi.org/10.3390/en18154129 - 4 Aug 2025
Abstract
This study empirically evaluates the energy and carbon reduction effects of an envelope retrofit applied to an aging public building in South Korea. Unlike previous studies that primarily relied on simulation-based analyses, this work fills the empirical research gap by using actual utility [...] Read more.
This study empirically evaluates the energy and carbon reduction effects of an envelope retrofit applied to an aging public building in South Korea. Unlike previous studies that primarily relied on simulation-based analyses, this work fills the empirical research gap by using actual utility billing data collected over one pre-retrofit year (2019) and two post-retrofit years (2023–2024). The retrofit included improvements to exterior walls, roofs, and windows, aiming to enhance thermal insulation and airtightness. The analysis revealed that monthly electricity consumption was reduced by 14.7% in 2023 and 8.0% in 2024 compared to that in the baseline year, with corresponding decreases in electricity costs and carbon dioxide emissions. Seasonal variations were evident: energy savings were significant in the winter due to reduced heating demand, while cooling energy use slightly increased in the summer, likely due to diminished solar heat gains resulting from improved insulation. By addressing both heating and cooling impacts, this study offers practical insights into the trade-offs of envelope retrofitting. The findings contribute to the body of knowledge by demonstrating the real-world performance of retrofit technologies and providing data-driven evidence that can inform policies and strategies for improving energy efficiency in public buildings. Full article
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16 pages, 3226 KiB  
Article
Sustainable Agronomical Practices Affect Essential Oil Composition of Tanacetum balsamita L.
by Martina Grattacaso, Alessandra Bonetti, Sara Di Lonardo and Luigi Paolo D’Acqui
Plants 2025, 14(15), 2406; https://doi.org/10.3390/plants14152406 - 3 Aug 2025
Viewed by 243
Abstract
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination [...] Read more.
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination (bioinoculants + compost), and a control. At each harvest, essential oil was extracted from fresh leaves via stem-flow distillation and analyzed using gas chromatography coupled with single quadrupole mass spectrometry. Twenty to twenty-four compounds were identified. Based on the dominant terpene derivative, the results indicated that Tanacetum balsamita L. cultivated in Italy belongs to “camphor” chemotype, a pharmacologically active compound known for its antimicrobial, anti-inflammatory, and analgesic properties. Moreover, three compounds, α-, β-phellandrene and myrtenol, were identified as typical of Tanacetum balsamita L. cultivated in Italy. Treatment effects were significant for some compounds (camphor, borneol, terpinen-4-ol, α-terpineol, dehydro sabinene ketone, and 3-thujanol), and the interaction between treatment and year was significant for a few compounds (borneol, terpinen-4-ol, dehydro sabinene ketone, 1,8-cineol, and 3-thujanol). These results emphasize the need to account for seasonal variation and underline the necessity of a deeper understanding of how experimental factors interact with them, especially in long-term essential oil studies. Full article
(This article belongs to the Special Issue Chemical Analysis, Bioactivity, and Application of Essential Oils)
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21 pages, 7111 KiB  
Article
Seasonal Variation in Energy Balance, Evapotranspiration and Net Ecosystem Production in a Desert Ecosystem of Dengkou, Inner Mongolia, China
by Muhammad Zain Ul Abidin, Huijie Xiao, Sanaullah Magsi, Fang Hongxin, Komal Muskan, Phuocthoi Hoang and Muhammad Azher Hassan
Water 2025, 17(15), 2307; https://doi.org/10.3390/w17152307 - 3 Aug 2025
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Abstract
This study investigates the seasonal dynamics of energy balance, evapotranspiration (ET), and Net Ecosystem Production (NEP) in the Dengkou desert ecosystem of Inner Mongolia, China. Using eddy covariance and meteorological data from 2019 to 2022, the research focuses on understanding how these processes [...] Read more.
This study investigates the seasonal dynamics of energy balance, evapotranspiration (ET), and Net Ecosystem Production (NEP) in the Dengkou desert ecosystem of Inner Mongolia, China. Using eddy covariance and meteorological data from 2019 to 2022, the research focuses on understanding how these processes interact in one of the world’s most water-limited environments. This arid research area received an average of 109.35 mm per annum precipitation over the studied period, classifying the region as a typical arid ecosystem. Seasonal patterns were observed in daily air temperature, with extremes ranging from −20.6 °C to 29.6 °C. Temporal variations in sensible heat flux (H), latent heat flux (LE), and net radiation (Rn) peaked during summer season. The average ground heat flux (G) was mostly positive throughout the observation period, indicating heat transmission from atmosphere to soil, but showed negative values during the winter season. The energy balance ratio for the studied period was in the range of 0.61 to 0.80, indicating challenges in achieving energy closure and ecological shifts. ET exhibited two annual peaks influenced by vegetation growth and climate change, with annual ET exceeding annual precipitation, except in 2021. Net ecosystem production (NEP) from 2019 to 2020 revealed that the Dengkou desert were a net source of carbon, indicating the carbon loss from the ecosystem. In 2021, the Dengkou ecosystem shifted to become a net carbon sink, effectively sequestrating carbon. However, this was sharply reversed in 2022, resulting in a significant net release of carbon. The study findings highlight the complex interactions between energy balance components, ET, and NEP in desert ecosystems, providing insights into sustainable water management and carbon neutrality strategies in arid regions under climate change effect. Full article
(This article belongs to the Special Issue The Observation and Modeling of Surface Air Hydrological Factors)
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19 pages, 1363 KiB  
Article
Non-Structural Carbohydrate Concentration Increases and Relative Growth Decreases with Tree Size in the Long-Lived Agathis australis (D.Don) Lindl.
by Julia Kaplick, Benjamin M. Cranston and Cate Macinnis-Ng
Forests 2025, 16(8), 1270; https://doi.org/10.3390/f16081270 - 3 Aug 2025
Viewed by 168
Abstract
The southern conifer Agathis australis (D.Don) Lindl. is a large and long-lived species endemic to Aotearoa New Zealand. It is threatened due to past logging activities, pathogen attack and potentially climate change, with increasing severity and frequency of drought and heatwaves across its [...] Read more.
The southern conifer Agathis australis (D.Don) Lindl. is a large and long-lived species endemic to Aotearoa New Zealand. It is threatened due to past logging activities, pathogen attack and potentially climate change, with increasing severity and frequency of drought and heatwaves across its distribution. Like many large tree species, little is known about the carbon dynamics of this ecologically and culturally significant species. We explored seasonal variations in non-structural carbohydrates (NSCs) and growth in trees ranging from 20 to 175 cm diameter at breast height (DBH). NSCs were seasonally stable with no measurable pattern across seasons. However, we found growth rates standardised to basal area and sapwood area (growth efficiency) declined with tree age and stem NSC concentrations (including total NSCs, sugars and starch) all increased as trees aged. Total NSC concentrations were 0.3%–0.6% dry mass for small trees and 0.8%–1.8% dry mass for larger trees, with strong relationships between DBH and total NSC, sugar and starch in stems but not roots. Cumulative growth efficiency across the two-year study period declined as tree size increased. Furthermore, there was an inverse relationship between growth efficiency across the two-year study period and NSC concentrations of stems. This relationship was driven by differences in carbon dynamics in trees of different sizes, with trees progressing to a more conservative carbon strategy as they aged. Simultaneously declining growth efficiency and increasing NSC concentrations as trees age could be evidence for active NSC accumulation to buffer against carbon starvation in larger trees. Our study provides new insights into changing carbon dynamics as trees age and may be evidence for active carbon accumulation in older trees. This may provide the key for understanding the role of carbon processes in tree longevity. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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