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18 Results Found

  • Article
  • Open Access
1 Citations
3,428 Views
29 Pages

Proteomic Analysis of Proteins Related to Defense Responses in Arabidopsis Plants Transformed with the rolB Oncogene

  • Yulia V. Vereshchagina,
  • Anastasiya A. Mironova,
  • Dmitry V. Bulgakov and
  • Victor P. Bulgakov

18 January 2023

During Agrobacterium rhizogenes–plant interaction, the rolB gene is transferred into the plant genome and is stably inherited in the plant’s offspring. Among the numerous effects of rolB on plant metabolism, including the activation of se...

  • Article
  • Open Access
682 Views
27 Pages

rolB Promotes Adventitious Root Development in Pyrus betulaefolia by Modulating Endogenous Hormones and Gene Expression

  • Ting Xie,
  • Weimin Wang,
  • Kuozhen Nie,
  • Zijuan He,
  • Jiaojiao He,
  • Yuxing Zhang,
  • Na Liu and
  • Yingli Li

11 September 2025

We investigated the effect of Agrobacterium rhizogenes-mediated transformation mof rolB on adventitious root development and endogenous hormones in ‘duli’ (Pyrus betulaefolia) via transcriptomic analysis of wild-type (WT) and rolB-transfo...

  • Article
  • Open Access
12 Citations
3,550 Views
17 Pages

Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures

  • Yury N. Shkryl,
  • Galina K. Tchernoded,
  • Yulia A. Yugay,
  • Valeria P. Grigorchuk,
  • Maria R. Sorokina,
  • Tatiana Y. Gorpenchenko,
  • Olesya D. Kudinova,
  • Anton I. Degtyarenko,
  • Maria S. Onishchenko and
  • Victor P. Bulgakov
  • + 2 authors

Aristolochia manshuriensis is a relic liana, which is widely used in traditional Chinese herbal medicine and is endemic to the Manchurian floristic region. Since this plant is rare and slow-growing, alternative sources of its valuable compounds could...

  • Article
  • Open Access
17 Citations
3,103 Views
17 Pages

Biodiversity of rolB/C-like Natural Transgene in the Genus Vaccinium L. and Its Application for Phylogenetic Studies

  • Roman Zhidkin,
  • Peter Zhurbenko,
  • Olesya Bogomaz,
  • Elizaveta Gorodilova,
  • Ivan Katsapov,
  • Dmitry Antropov and
  • Tatiana Matveeva

A variety of plant species found in nature contain agrobacterial T-DNAs in their genomes which they transmit in a series of sexual generations. Such T-DNAs are called cellular T-DNAs (cT-DNAs). cT-DNAs have been discovered in dozens of plant genera,...

  • Article
  • Open Access
9 Citations
2,936 Views
17 Pages

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

  • Elena A. Vasyutkina,
  • Yulia A. Yugay,
  • Valeria P. Grigorchuk,
  • Olga V. Grishchenko,
  • Maria R. Sorokina,
  • Yulia L. Yaroshenko,
  • Olesya D. Kudinova,
  • Varvara D. Stepochkina,
  • Victor P. Bulgakov and
  • Yury N. Shkryl

1 December 2022

Ipomoea batatas is a vital root crop and a source of caffeoylquinic acid derivatives (CQAs) with potential health-promoting benefits. As a naturally transgenic plant, I. batatas contains cellular T-DNA (cT-DNA) sequence homologs of the Agrobacterium...

  • Article
  • Open Access
17 Citations
4,594 Views
18 Pages

Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants

  • Samina Rubnawaz,
  • Mohammad K. Okla,
  • Nosheen Akhtar,
  • Imdad Ullah Khan,
  • Muhammad Zeeshan Bhatti,
  • Hong-Quan Duong,
  • Mohamed A. El-Tayeb,
  • Yahaya B. Elbadawi,
  • Khalid S. Almaary and
  • Bushra Mirza
  • + 2 authors

13 September 2021

Herbal and traditional medicines can play a pivotal role in combating cancer and neglected tropical diseases. Ajuga bracteosa, family Lamiaceae, is an important medicinal plant. The genetic transformation of A. bracteosa with rol genes of Agrobacteri...

  • Article
  • Open Access
2 Citations
3,010 Views
20 Pages

Chemical Profile of Cell Cultures of Kalanchoë gastonis-bonnieri Transformed by Agrobacterium rhizogenes

  • María Guadalupe Barrera Núñez,
  • Mónica Bueno,
  • Miguel Ángel Molina-Montiel,
  • Lorena Reyes-Vaquero,
  • Elena Ibáñez and
  • Alma Angélica Del Villar-Martínez

15 January 2024

Kalanchoë gastonis-bonnieri Raym.-Hamet & Perrier is a plant used for medicinal purposes in the treatment of several ailments. The aim of this study was to analyze the chemical profile of extracts from K. gastonis-bonnieri embryogenic calli,...

  • Article
  • Open Access
4 Citations
1,664 Views
13 Pages

Establishment of Dittrichia viscosa L. Hairy Roots and Improvement of Bioactive Compound Production

  • Annalisa Paradiso,
  • Miriana Durante,
  • Sofia Caretto and
  • Angelo De Paolis

18 November 2024

Dittrichia viscosa is a ruderal plant species growing along roadsides and well adapting to extreme environmental conditions. D. viscosa plant tissues, especially leaves, are known to be a rich source of bioactive metabolites which have antioxidant, c...

  • Article
  • Open Access
3 Citations
2,284 Views
23 Pages

(-)-5-Demethoxygrandisin B a New Lignan from Virola surinamensis (Rol.) Warb. Leaves: Evaluation of the Leishmanicidal Activity by In Vitro and In Silico Approaches

  • Steven Souza Paes,
  • João Victor Silva-Silva,
  • Paulo Wender Portal Gomes,
  • Luely Oliveira da Silva,
  • Ana Paula Lima da Costa,
  • Manoel Leão Lopes Júnior,
  • Daiana de Jesus Hardoim,
  • Carla J. Moragas-Tellis,
  • Noemi Nosomi Taniwaki and
  • Kátia da Silva Calabrese
  • + 4 authors

Leishmaniasis is a complex disease caused by infection with different Leishmania parasites. The number of medications used for its treatment is still limited and the discovery of new drugs is a valuable approach. In this context, here we describe the...

  • Article
  • Open Access
8 Citations
2,245 Views
14 Pages

Effect of Light Conditions on Polyphenol Production in Transformed Shoot Culture of Salvia bulleyana Diels

  • Marta Krzemińska,
  • Katarzyna Hnatuszko-Konka,
  • Izabela Weremczuk-Jeżyna,
  • Aleksandra Owczarek-Januszkiewicz,
  • Wiktoria Ejsmont,
  • Monika A. Olszewska and
  • Izabela Grzegorczyk-Karolak

Various strategies have been used to increase the efficiency of secondary metabolite production in Salvia plants. This report is the first to examine the spontaneous development of Salvia bulleyana shoots transformed by Agrobacterium rhizogenes on ha...

  • Article
  • Open Access
3 Citations
3,070 Views
11 Pages

Characterization of Carnivorous Plants Sarracenia purpurea L. Transformed with Agrobacterium rhizogenes

  • Kinga Maria Pilarska,
  • Manuela Panić,
  • Ivana Radojčić Redovniković and
  • Magdalena Wróbel-Kwiatkowska

13 October 2022

People have used carnivorous plants of the genus Sarracenia in folk medicine for centuries due to the biochemical composition of Sarracenia plants, which are rich in numerous bioactive compounds with anti-inflammatory, antioxidant, antiviral and anti...

  • Article
  • Open Access
552 Views
17 Pages

Functional Analysis of Naturally Integrated Rol Genes in Sweet Potato via CRISPR/Cas9 Genome Editing

  • Yury Shkryl,
  • Yulia Yaroshenko,
  • Valeria Grigorchuk,
  • Victor Bulgakov and
  • Yulia Yugay

5 December 2025

Sweet potato (Ipomoea batatas) is a globally important crop and one of a growing number of plants recognized as naturally transgenic, harboring Agrobacterium-derived T-DNA genes whose functions remain largely uncharacterized. In this proof-of-concept...

  • Article
  • Open Access
18 Citations
7,610 Views
11 Pages

10 August 2020

Previous efforts to transform lentil have been considerably hampered by the crop’s recalcitrant nature, giving rise to particularly low transformation and regeneration frequencies. This study aimed at optimizing an Agrobacterium rhizogenes-mediated t...

  • Review
  • Open Access
20 Citations
5,533 Views
13 Pages

6 December 2018

The capability of the soil bacterium Agrobacterium rhizogenes to reprogram plant development and induce adventitious hairy roots relies on the expression of a few root-inducing genes (rol A, B, C and D), which can be transferred from large virulence...

  • Article
  • Open Access
10 Citations
2,907 Views
15 Pages

Induction, Flavonoids Contents, and Bioactivities Analysis of Hairy Roots and True Roots of Tetrastigma hemsleyanum Diels et Gilg

  • Hongzhen Wang,
  • Anran Wang,
  • Hanying Pu,
  • Yuxin Yang,
  • Zeyuan Ling,
  • Haishun Xu,
  • Juan Xu,
  • Haizheng Yu and
  • Xueqian Wu

16 March 2023

The flavonoids in Tetrastigma hemsleyanum Diels et Gilg (T. hemsleyanum) have high medicinal value. However, because of slow growth and harsh ecological environments, T. hemsleyanum is currently an endangered species. In light of this, we present a d...

  • Article
  • Open Access
1 Citations
1,409 Views
17 Pages

In Arctic regions, ship structures face low temperatures, overloads, thickness effects, and fluctuating stress ratios, which significantly influence the fatigue crack growth (FCG) behavior of marine steels. This study investigates the FCG behaviors o...

  • Article
  • Open Access
1 Citations
2,016 Views
24 Pages

The Overload-Induced Delay Model of 7055 Aluminum Alloy Under Periodic Overloading

  • Zuoting Liu,
  • Jing Cao,
  • Shilong Liu,
  • Yuqi Yang and
  • Weixing Yao

9 June 2025

Aluminum alloys, serving as critical structural materials in the aviation and aerospace industry, frequently endure variable amplitude loading under complex service conditions. The resulting non-steady-state crack propagation behavior directly impact...

  • Article
  • Open Access
1 Citations
1,425 Views
20 Pages

Enhancement of Rosmarinic Acid Production in Hairy Root Cultures of Perovskia atriplicifolia Benth

  • Renata Grąbkowska,
  • Marta Krzemińska,
  • Katarzyna Gaweda-Walerych,
  • Anna Karolina Kiss,
  • Kamila Pluta and
  • Izabela Grzegorczyk-Karolak

This study reports the first successful establishment of Perovskia atriplicifolia hairy root cultures using Rhizobium rhizogenes and evaluates their potential for bioactive phenolic acid production, particularly rosmarinic acid (RA). Hairy roots were...