Search Results (3,833)

The administration of isolated mitochondria is a promising strategy for protecting cells from oxidative damage. This study aimed to identify mitochondrial characteristics that contribute to stronger protective effects. We compared two types of mitochondria isolated from C6 cells with similar ATP-producing capacity but differing in outer membrane integrity. To evaluate their stability in extracellular conditions, we examined their behavior in serum. Both types underwent mitochondrial permeability transition to a similar extent; however, under intracellular-like conditions after serum incubation, mitochondria with intact membranes retained more polarized mitochondria. Notably, mitochondria with intact outer membranes were internalized more efficiently than those with damaged membranes. In H9c2 cells, both types of mitochondria similarly increased intracellular ATP levels 1 h after administration under all tested conditions. When co-administered with H₂O₂, both suppressed oxidative damage to a comparable degree, as indicated by similar H₂O₂-scavenging activity in solution, comparable intracellular ROS levels, and equivalent preservation of electron transport chain activity. However, at higher H₂O₂ concentrations, cells treated with mitochondria possessing intact outer membranes exhibited greater survival 24 h after co-administration. Furthermore, when mitochondria were added after H₂O₂-induced damage and their removal, intact mitochondria conferred superior cell survival compared to damaged ones. These findings suggest that while both mitochondrial types exert comparable antioxidant effects, outer membrane integrity prior to administration plays a critical role in enhancing cell survival under conditions of oxidative stress. Full article

Ecosystem services—the benefits humans derive from nature—are foundational to environmental sustainability and economic well-being, with carbon sequestration and storage standing out as critical regulating services in the fight against climate change. This study presents a comprehensive financial valuation of the carbon sequestration, storage and product substitution ecosystem services provided by the Hungarian forest-based sector. Using a multi-scenario framework, four complementary valuation concepts are assessed: total carbon storage (biomass, soil, and harvested wood products), annual net sequestration, emissions avoided through material and energy substitution, and marketable carbon value under voluntary carbon market (VCM) and EU Carbon Removal Certification Framework (CRCF) mechanisms. Data sources include the National Forestry Database, the Hungarian Greenhouse Gas Inventory, and national estimates on substitution effects and soil carbon stocks. The total carbon stock of Hungarian forests is estimated at 1289 million tons of CO₂ eq, corresponding to a theoretical climate liability value of over EUR 64 billion. Annual sequestration is valued at approximately 380 million EUR/year, while avoided emissions contribute an additional 453 million EUR/year in mitigation benefits. A comparative analysis of two mutually exclusive crediting strategies—improved forest management projects (IFMs) avoiding final harvesting versus long-term carbon storage through the use of harvested wood products—reveals that intensified harvesting for durable wood use offers higher revenue potential (up to 90 million EUR/year) than non-harvesting IFM scenarios. These findings highlight the dual role of forests as both carbon sinks and sources of climate-smart materials and call for policy frameworks that integrate substitution benefits and long-term storage opportunities in support of effective climate and bioeconomy strategies. Full article

This study focuses on the exchange of mono- and divalent metal cations in FAU-type zeolite and their behavior in gas-phase CO₂ adsorption measurements and liquid-phase methylene blue (MB) adsorption in the absence of oxidizing agents under dark conditions. Firstly, zeolites exchanged with different cations were characterized by several techniques, such as XRD, SEM, XRF, XPS, and N₂ adsorption–desorption, to reveal the impact of the cations on the zeolite texture and structure. The adsorption studies revealed a positive effect of cation exchange on the adsorption capacity of the zeolite, particularly for silver-loaded FAU zeolite. In liquid-phase experiments, Ag-Y zeolite also demonstrated the highest MB removal, with a value of 79 mg/g. Kinetic studies highlighted that Ag-Y could reach the MB adsorption equilibrium within 1 h, with its highest rate of adsorption occurring during the first 5 min. In gas-phase adsorption studies, the highest CO₂ adsorption capacity was also achieved over Ag-Y, yielding 10.4 µmol/m² of CO₂ captured. Full article

The use of Internet of Things (IoT) for sports innovation has transformed the way athletes train, compete, and recover in any sports activity. This study performs a bibliometric analysis to examine research trends, collaborations, and publications in the realm of IoT and Sports. Our analysis included 780 Scopus articles and 150 WoS articles published during 2012–2025, and duplicates were removed. We analyzed and visualized the bibliometric data using R version 3.6.1, VOSviewer version 1.6.20, and the bibliometrix library. The study provides insights from a bibliometric analysis, showcasing the allocation of topics, scientific contributions, patterns of co-authorship, prominent authors and their productivity over time, notable terms, key sources, publications with citations, analysis of citations, source-specific citation analysis, yearly publication patterns, and the distribution of research papers. The results indicate that China and India have the leading scientific production in the development of IoT and Sports research, with prominent authors like Anton Umek, Anton Kos, and Emiliano Schena making significant contributions. Wearable technology and wearable sensors are the most trending topics in IoT and Sports, followed by medical sciences and artificial intelligence paradigms. The analysis also emphasizes the importance of open-access journals like ‘Journal of Physics: Conference Series’ and ‘IEEE Access’ for their contributions to IoT and Sports research. Future research directions focus on enhancing effective, lightweight, and efficient wearable devices while implementing technologies like edge computing and lightweight AI in wearable technologies. Full article

This study proposes a laser texturing method to optimize adhesion and minimize residual stresses in CVD diamond films deposited on tungsten carbide (WC-Co). WC-5.8 wt% Co substrates were textured with quadrangular pyramidal patterns (35 µm) using a 1064 nm nanosecond-pulsed laser, followed by chemical treatment (Murakami’s solution + aqua regia) to remove surface cobalt. Diamond films were grown via HFCVD and characterized by Raman spectroscopy, EDS, and Rockwell indentation. The results demonstrate that pyramidal texturing increased the surface area by a factor of 58, promoting effective mechanical interlocking and reducing compressive stresses to −1.4 GPa. Indentation tests revealed suppression of interfacial cracks, with propagation paths deflected toward textured regions. The pyramidal geometry exhibited superior cutting post-deposition cooling time for stress relief from 3 to 1 h. These findings highlight the potential of laser texturing for high-performance machining tool applications. Full article

In response to the European Union’s revised Urban Wastewater Treatment Directive, which mandates enhanced monitoring and advanced treatment of micropollutants, this study was conducted. It took place within the Coastal Landscape Park (CLP), a Natura 2000 protected area in northern Poland. The focus was on the municipal wastewater treatment plant in Jastrzębia Góra, located in a region exposed to seasonal tourist pressure and discharging effluent into the Czarna Wda River. A total of 90 wastewater samples were collected during five monitoring campaigns (July, September 2021; February, May, July 2022) and analysed for 13 pharmaceuticals and personal care products (PPCPs) using ultra-high-performance liquid chromatography tandem mass spectrometry with electrospray ionisation (UHPLC-ESI-MS/MS). The monitoring included both untreated (UTWW) and treated wastewater (TWW) to assess the PPCP removal efficiency and persistence. The highest concentrations in the treated wastewater were observed for metoprolol (up to 472.9 ng/L), diclofenac (up to 3030 ng/L), trimethoprim (up to 603.6 ng/L) and carbamazepine (up to 2221 ng/L). A risk quotient (RQ) analysis identified diclofenac and LI-CBZ as priority substances for monitoring. Multivariate analyses (PCA, HCA) revealed co-occurrence patterns and seasonal trends. The results underline the need for advanced treatment solutions and targeted monitoring, especially in sensitive coastal catchments with variable micropollutant presence. Full article

Fibrate pharmaceuticals (fibrates), as a widespread class of emerging contaminants, pose potential risks to both ecological systems and human health. The photocatalytic system based on nitrogen (N) and fluorine (F) co-doped TiO₂ nanotube arrays (NF-TNAs) provides a renewable solution for fibrate pharmaceutical removal from water, powered by inexhaustible sunlight. In this study, the degradation of two typical fibrates, i.e., bezafibrate (BZF) and ciprofibrate (CPF), under simulated sunlight irradiation through NF-TNAs were investigated. The photocatalytic degradation of BZF/CPF was achieved through combined radical and non-radical oxidation processes, while the generation and reaction mechanisms of associated reactive oxygen species (ROS) were examined. Electron paramagnetic resonance detection and quenching tests confirmed the existence of h⁺, •OH, O₂^•−, and ¹O₂, with O₂^•− playing the predominant role. The transformation products (TPs) of BZF/CPF were identified through high-resolution mass spectrometry analysis combined with quantum chemical calculations to elucidate the degradation pathways. The influence of co-existing ions and typical natural organic matters (NOM) on BZF/CPF degradation were also tested. Eventually, the ecological risk of BZF/CPF transformation products was assessed through quantitative structure–activity relationship (QSAR) modeling, and the results showed that the proposed photocatalytic system can largely alleviate fibrate toxicity. Full article

The background for this study is the limitations of the conventional approach of using deforestation area multiplied by biomass densities or emission factors. We demonstrated how TanDEM-X and GEDI data can be combined to estimate forest Above Ground Biomass (AGB) change at the national scale for Tanzania. The results can be further recalculated to estimate CO₂ emissions and removals from the forest. We used repeated short wavelength, InSAR DEMs from TanDEM-X to derive changes in forest canopy height and combined this with GEDI data to convert such height changes to AGB changes. We estimated AGB change during 2012–2019 to be −2.96 ± 2.44 MT per year. This result cannot be validated, because the true value is unknown. However, we corroborated the results by comparing with other approaches, other datasets, and the results of other studies. In conclusion, TanDEM-X and GEDI can be combined to derive reliable temporal change in AGB at large scales such as a country. An important advantage of the method is that it is not required to have a representative field inventory plot network nor a full coverage DTM. A limitation for applying this method now is the lack of frequent and systematic InSAR elevation data. Full article

Engineering of Cupriavidus necator could enable the production of various polyhydroxyalkanoates (PHAs); particularly, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)), a biopolymer with enhanced mechanical and thermal properties compared to poly(3-hydroxybutyrate) (PHB), can be efficiently produced from vegetable oils. However, challenges remain in the recovery process, particularly in removing residual oil and minimizing degradation of the polymer structure during extraction steps. This study investigated the effects of ethanol-based defatting on the recovery and polymeric properties of P(3HB-co-3HH). The proposed method involves the addition of ethanol to the cell broth to effectively remove residual oil. Ethanol improved the separation of microbial cells from the broth, thereby streamlining the downstream recovery process. Using ethanol in the washing step increased the recovery yield and purity to 95.7% and 83.4%, respectively (compared to 87.4% and 76.2% for distilled water washing), representing improvements of 8.3% and 7.2%. Ethanol washing also resulted in a 19% higher molecular weight compared to water washing, indicating reduced polymer degradation. In terms of physical properties, the elongation at break showed a significant difference: 241.9 ± 27.0% with ethanol washing compared to water (177.7 ± 10.3%), indicating ethanol washing retains flexibility. Overall, an ethanol washing step for defatting could simplify the recovery steps, increase yield and purity, and retain mechanical properties, especially for P(3HB-co-3HH) from oils. Full article

Iopamidol (IPM), as a typical recalcitrant emerging pollutant and precursor of iodinated disinfection by-products (I-DBPs), is unsuccessfully removed by conventional wastewater treatment processes. This study comprehensively evaluated the ozone/peracetic acid (O₃/PAA) process for IPM degradation, focusing on degradation kinetics, environmental impacts, transformation products, ecotoxicity, disinfection byproducts (DBPs), and microbial inactivation. The O₃/PAA system synergistically activates PAA via O₃ to generate hydroxyl radicals (^•OH) and organic radicals (CH₃COO^• and CH₃CO(O)O^•), achieving an IPM degradation rate constant of 0.10 min⁻¹, which was significantly higher than individual O₃ or PAA treatments. The degradation efficiency of IPM in the O₃/PAA system exhibited a positive correlation with solution pH, achieving a maximum degradation rate constant of 0.23 min⁻¹ under alkaline conditions (pH 9.0). Furthermore, the process demonstrated strong resistance to interference from coexisting anions, maintaining robust IPM removal efficiency in the presence of common aqueous matrix constituents. Furthermore, quenching experiments revealed ^•OH dominated IPM degradation in O₃/PAA system, while the direct oxidation by O₃ and R-O^• played secondary roles. Additionally, based on transformation products (TPs) identification and ECOSAR predictions, the primary degradation pathways were elucidated and the potential ecotoxicity of TPs was systematically assessed. DBPs analysis after chlorination revealed that the O₃/PAA (2.5:3) system achieved the lowest total DBPs concentration (99.88 μg/L), representing a 71.5% reduction compared to PAA alone. Amongst, dichloroacetamide (DCAM) dominated the DBPs profile, comprising > 60% of total species. Furthermore, the O₃/PAA process achieved rapid 5–6 log reductions of E. coli. and S. aureus within 3 min. These results highlight the dual advantages of O₃/PAA in effective disinfection and byproduct control, supporting its application in sustainable wastewater treatment. Full article

The growing environmental concerns associated with petroleum-based plastics require the development of sustainable, biodegradable alternatives. Polyhydroxyalkanoates (PHAs), a family of biodegradable bioplastics, offer a promising potential as eco-friendly substitutes due to their renewable origin and favorable degradation properties. This research investigates the use of synthetic condensate, mimicking the liquid fraction from drying and shredding of household food waste, as a viable substrate for PHA production using mixed microbial cultures. Two draw-fill reactors (DFRs) were operated under different feed organic concentrations (2.0 ± 0.5 and 3.8 ± 0.6 g COD/L), maintaining a consistent carbon-to-nitrogen ratio to selectively enrich microorganisms capable of accumulating PHAs through alternating nutrient availability and deficiency. Both reactors achieved efficient organic pollutant removal (>95% soluble COD removal), stable biomass growth, and optimal pH levels. Notably, the reactor with the higher organic load (DFR-2) demonstrated a modest increase in PHA accumulation (19.05 ± 7.18%) compared to the lower-loaded reactor (DFR-1; 15.19 ± 6.00%), alongside significantly enhanced biomass productivity. Polymer characterization revealed the formation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), influenced by the substrate composition. Microbial community analysis showed an adaptive shift towards Proteobacteria dominance, signifying successful enrichment of effective PHA producers. Full article

Direct air capture (DAC), as a complementary strategy to carbon capture and storage (CCS), offers a scalable and sustainable pathway to remove CO₂ directly from the ambient air. This study presents a detailed evaluation of the amine-functionalised metal-organic framework (MOF) sorbent, mmen-Mg₂(dobpdc), for DAC using a temperature–vacuum swing adsorption (TVSA) process. While this sorbent has demonstrated promising performance in point-source CO₂ capture, this is the first dynamic simulation-based study to rigorously assess its effectiveness for low-concentration atmospheric CO₂ removal. A transient one-dimensional TVSA model was developed in Aspen Adsorption and validated against experimental breakthrough data to ensure accuracy in capturing both the sharp and gradual adsorption kinetics. To enhance process efficiency and sustainability, this work provides a comprehensive parametric analysis of key operational factors, including air flow rate, temperature, adsorption/desorption durations, vacuum pressure, and heat exchanger temperature, on process performance, including CO₂ purity, recovery, productivity, and specific energy consumption. Under optimal conditions for this sorbent (vacuum pressure lower than 0.15 bar and feed temperature below 15 °C), the TVSA process achieved ~98% CO₂ purity, recovery over 70%, and specific energy consumption of about 3.5 MJ/KgCO₂. These findings demonstrate that mmen-Mg₂(dobpdc) can achieve performance comparable to benchmark DAC sorbents in terms of CO₂ purity and recovery, underscoring its potential for scalable DAC applications. This work advances the development of energy-efficient carbon removal technologies and highlights the value of step-shape isotherm adsorbents in supporting global carbon-neutrality goals. Full article

The CRISPR/Cas9 system is a powerful genome-editing tool that is applied in baculovirus engineering. In this study, we present the first report of the AcMNPV genome deletions for bioproduction purposes, using a dual single-guide RNA (sgRNA) CRISPR/Cas9 approach. We used this method to remove nonessential genes for the budded virus and boost recombinant protein yields when applied as BEVS. We show that the co-delivery of two distinct ribonucleoprotein (RNP) complexes, each assembled with a sgRNA and Cas9, into Sf9 insect cells efficiently generated deletions of fragments containing tandem genes in the genome. To evaluate the potential of this method, we assessed the expression of two model proteins, eGFP and HRPc, in insect cells and larvae. The gene deletions had diverse effects on protein expression: some significantly enhanced it while others reduced production. These results indicate that, although the targeted genes are nonessential, their removal can differentially affect recombinant protein yields depending on the host. Notably, HRPC expression increased up to 3.1-fold in Spodoptera frugiperda larvae. These findings validate an effective strategy for developing minimized baculovirus genomes and demonstrate that dual-guide CRISPR/Cas9 editing is a rapid and precise tool for baculovirus genome engineering. Full article

Enhanced weathering of silicate rocks in agriculture is an option for atmospheric CO₂ removal and fertility improvement. The objective of our work is to characterise some of the agricultural consequences of a basaltic powder amendment on soil-crop systems. Two doses of basalt (80 and 160 t ha⁻¹) were applied to two types of slightly acid soils (sandy or silty clayey), derived from long-term trials at Bordeaux (INRAE, France) and Rothamsted Research (England), respectively. For each soil, half of the pots were planted with ryegrass; the other half were left bare. Thus, the experiment had twelve treatments with four replications per treatment. Soil pH increased with the addition of basalt (+0.8 unit), with a 5% equivalence of that of reactive chalk. The basalt contained macro- and micronutrients. Some cations extractable in the basalt before being mixed to the soil became more extractable with increased weathering, independent of plant cover. Plant uptake generally increased for macronutrients and decreased for micronutrients, due to increased stock (macro) and reduced availability (micronutrients and P), related to pH increases. K supplied in the basalt was responsible for a significant increase in plant yield on the sandy soil, linked to an average basalt K utilisation efficiency of 33%. Our general conclusion is that rock dust applications have to be re-evaluated at each site with differing soil characteristics. Full article

Mycelium-bonded composites (MBCs), or myco-composites, represent a novel engineered material that combines natural lignocellulosic substrates with a fungal matrix. As a sustainable alternative to plastics, MBCs are gaining increasing interest; however, their large-scale industrial adoption remains limited, partly due to low social acceptance resulting from their unattractive appearance. Laser engraving provides a promising method for fabricating intricate patterns and functional surfaces on MBCs, minimizing tool wear, material loss, and environmental impact, while enhancing esthetic and engineering properties. This study investigates the influence of CO₂ laser parameters on the material removal rate during the engraving of myco-composites, focusing on the effects of variable laser power, beam defocus, and head feed rate on engraving outcomes. The results demonstrate that laser power and beam focus significantly impact material removal in mycelium-bonded composites. Specifically, increasing the laser power results in greater material removal, which is more pronounced when the beam is focused due to higher energy density. In contrast, a beam defocused by 1 mm produces less intense material removal. These findings highlight the critical role of beam focus—surpassing the influence of power alone—in determining engraving quality, particularly on irregular or uneven surfaces. Moreover, reducing the laser head feed rate at a constant power level increases the material removal rate linearly; however, it also results in excessive charring and localized overheating, revealing the low thermal tolerance of myco-composites. These insights are essential for optimizing laser processing techniques to fully realize the potential of mycelium-bonded composites as sustainable engineering materials, simultaneously maintaining their appearance and functional properties. Full article