Search Results (112)

Chrysanthemum morifolium is an important ornamental crop whose flowering is strictly regulated by photoperiod. However, the molecular mechanisms linking photoperiod signals to floral meristem identity and organ development in Asteraceae remain unclear. In this study, we identified and characterized CmAFL1, a FUL-like MADS-box gene specific to the Asteraceae family, in the short-day cultivar ‘Jinba’. CmAFL1 expression is upregulated under short-day conditions, primarily expressed in the shoot apical meristem during flower bud differentiation and predominantly in the receptacle during organ development. RNA interference-mediated silencing of CmAFL1 resulted in delayed flowering, arrested inflorescence development, malformed tubular florets, plant dwarfism, and excessive lateral branching, indicating its pleiotropic roles in flowering time, plant architecture, and floral organ patterning. At the molecular level, yeast one-hybrid assays revealed that the photoperiod pathway transcription factor CmCOL4 directly binds to the promoter region of CmAFL1. Furthermore, yeast one-hybrid and dual-luciferase assays confirmed that CmAFL1 directly interacts with and activates the promoter of the E-class floral organ identity gene CmSEP3. Together, our findings identify a novel transcriptional regulatory module CmCOL4-CmAFL1-CmSEP3 that potentially links photoperiod signaling to floral meristem identity and organ specification in Chrysanthemums. While further in planta validation is warranted, these findings provide novel insights into photoperiodic flowering regulation in Asteraceae and may serve as a theoretical foundation for molecular breeding strategies targeting flowering time manipulation in ornamental Chrysanthemums. Full article

This study addresses the need for effective natural inhibitors of enzymatic browning in fresh-cut produce by exploring plant-derived compounds with activity against apple polyphenol oxidase (PPO). Extracts from 26 wild plant species were screened using thin-layer chromatography (TLC)-coupled bioautographic assays specifically adapted to apple PPO. Among these, D. fullonum showed clear inhibitory activity and was selected for further investigation. Extracts from different plant organs revealed that PPO inhibition was localized in the leaves, while stems and flower receptacles were inactive. Bioautography-guided fractionation of leaf extracts enabled the isolation of active fractions, and structural characterization by HRMS and NMR indicated that the activity is associated with the iridoid glycosides sylvestrosides III and IV. Antioxidant assays (DPPH, ABTS, CUPRAC) and TLC profiling demonstrated that PPO inhibition was not directly correlated with radical scavenging activity. In situ application of the leaf extract (0.5% w/v) on fresh-cut apple slices significantly delayed browning during refrigerated storage, showing comparable or improved performance relative to ascorbic acid at later stages. These results highlight D. fullonum as a promising source of natural antibrowning agents and demonstrate the utility of food-specific bioautographic assays for the targeted discovery of functional plant metabolites. Full article

Camellia oleifera Abel. is a typical self-incompatible plant, yet its molecular mechanisms have not been comprehensively elucidated, which constitutes the principal cause underlying the relatively low natural fruit set rate in C. oleifera. S-RNase belongs to the RNase T2 gene family and represents a class of glycoproteins specifically expressed in the style with certain “cytotoxicities” capable of degrading ribonucleic acid (RNA) in their own pollen tube cells, thereby inducing programmed cell death in pollen tube cells. To identify pistil S genes participating in the self-incompatibility response of C. oleifera, nine RNase T2 family genes were identified based on transcriptomic and whole-genome sequencing data of the camellia oil tree and designated CoRNS1–CoRNS9. Systematic evolutionary analysis revealed that CoRNS5, CoRNS7, and CoRNS8 exhibit close phylogenetic relationships with S-RNases of the Camelliaceae family, whereas CoRNS6 shows closer affinity with S-RNases of the Solanaceae family. Analysis of promoter cis-acting elements revealed that RNase T2 family genes are regulated by multiple hormones, including abscisic acid, methyl jasmonate, cytokinin, auxin, salicylic acid, and gibberellin, and possess MYB transcription factor-binding sites. Expression analysis revealed that CoRNS6 is expressed exclusively in the ovary; CoRNS5 is expressed at the highest level in the style, followed by lower levels in the petals, anthers, filaments, receptacle, and ovary; CoRNS1 is expressed in all tissues except anthers; and the other genes are expressed across various floral tissues. Ex vitro pollen culture system experiments demonstrated that the CoRNS1 and CoRNS5 recombinant proteins significantly inhibited the elongated growth of autogamous pollen tubes, whereas CoRNS7 significantly reduced the pollen germination rate. Fluorescence labeling revealed that treatment of autogamous pollen tubes with the recombinant proteins CoRNS1, CoRNS5, and CoRNS7 induced microfilament skeleton depolymerization and increased the Ca²⁺ concentration within the pollen tubes. Additionally, treatment with the CoRNS5 recombinant protein increased the reactive oxygen species (ROS) levels in autogamous pollen tubes. These findings suggest that RNase T2 family genes are involved in the self-incompatibility response in C. oleifera, with CoRNS1, CoRNS5, and CoRNS7 playing pivotal roles. Overall, our results not only offer a theoretical foundation for elucidating the regulatory network governing self-incompatibility in C. oleifera, but also furnish valuable genetic resources for future molecular breeding programs targeting improved self-compatibility and increased fruit yield. Full article

N. cadamba is a fast-growing and economically important tree species with significant industrial and medicinal values. Its spherical capitulum comprises hundreds of sessile florets densely aggregated on a receptacle. How this unique floral structure shapes the mating system of this species remains unknown, as this structure enhances the probabilities of both selfing and outcrossing. Here we clarify this question by investigating a natural population. We randomly sampled 11 half-sib families and 6 progenies per half-sib family and assayed samples with whole-genome resequencing. Population genomic analysis indicated that about 93.09% of 4,876,674 high-quality SNPs were under Hardy–Weinberg equilibrium, with a mean inbreeding coefficient of 0.0614 in the sample. Genome-wide nucleotide diversity (π) was 0.0024 on average. The linkage disequilibria ($r$²) decayed by half at approximately 2.31 kb between sites across genomes. Principal component analysis showed clear family structure among all samples. Analysis with independent SNPs and MLTR (Program for estimating multilocus outcrossing rate) showed that the multilocus outcrossing rate (t_m) and single-locus outcrossing rate (t_s) were 1.028 ± 0.048 and 1.015 ± 0.060, respectively, with an estimated difference between t_m and t_s of 0.013 ± 0.022. Analysis with BORICE (Bayesian outcrossing rate and inbreeding coefficient estimation) showed an outcrossing rate (t) of 0.948 ± 0.029 and an inbreeding coefficient (F) of 0.012 ± 0.010. Collectively, these results imply that N. cadamba could be essentially outcrossing, without selfing and biparental inbreeding. The potentially outcrossing system of N. cadamba implies differences from its close relative N. macrophylla (a mixed-mating system) in population structure, genome evolution, and natural geographical distribution. This finding also provided a useful reference for breeding and genetic improvement of this “miracle tree”. Full article

Energy transition has established hydrogen and compressed natural gas (CNG) as key alternatives for reducing greenhouse gas emissions and promoting sustainable mobility in the transport sector. However, the safe deployment of this infrastructure is essential to ensure that decarbonisation strategies remain environmentally, socially and operationally sustainable. Filling stations handling flammable gases may release hydrogen or CNG into open environments where ventilation alone cannot always prevent unacceptable risk situations in populated and industrial areas. Although the current regulatory framework sets out essential design requirements, including safety distances, this article argues that risk management must go beyond minimum compliance, reconsidering the location of detectors, gas dispersion behaviour and the definition of hazard zones. The analysis takes into account the rapid dilution of these gases under open air ventilation conditions and supports a more risk-based approach to infrastructure planning. Since gas supply facilities operating at 25 MPa, the hazard zone from the vehicle receptacle is estimated at 3 m for hydrogen and 1.7 m for CNG. These findings contribute to a safer and more sustainable transport energy infrastructure. Full article

In this contribution, we describe a new species from the Estación de Biología Chamela (IBUNAM), located in Jalisco, Mexico. Mastigoproctus claritae sp. nov. has a stridulatory organ like M. mexicanus and M. xetame. However, it is distinguished from these species by the presence of a proventral marginal smooth keel in the chelicerae, a retrolateral surface pedipalp femur with congregated and blunt cristulae, a prodorsal margin spine S3 that is longer than the other spines in males, and symmetric, peanut-shaped spermatheca seminal receptacles in females. Mastigoproctus claritae sp. nov. represents the twenty-third species of the genus and the twelfth species documented in Mexico. Full article

Manual assembly of multi-pin cable harnesses remains vulnerable to miswiring issues when conductors are visually indistinguishable. This paper presents an industrial case study of a quick-connect harness composed of two connectors (receptacle-type and pin-type) linked by 16 black conductors (2.5 mm² and 200 mm length), where the dominant failure mode is a two-wire swap that breaks correct pin-to-pin mapping and may cause downstream equipment damage. In the baseline state, end-of-line verification relies on visual inspection only (1 min/unit), resulting in an internal nonconformity rate of 4% (repairable). To achieve the operational goal of zero defects (zero escapes), we proposed and integrated an electronic pin-to-pin continuity and mapping fixture as a deterministic End-of-Line (EOL) quality-gate implementing poka-yoke logic (“no PASS—no shipment”) and enabling structured traceability records. Using a before–after workload model that includes a mandatory retest after rework, the fixture reduces test time to 0.33 min/unit. For a monthly volume of 1500 units, total quality workload (test + rework + retest) decreases from 31 h/month to 13.58 h/month, releasing 17.42 h/month. Global quality productivity increases from 48.39 units/h to 110.46 units/h (+128%). The proposed architecture couples deterministic electrical verification with data logging aligned to digital-thread and data-driven quality-management concepts to sustain continuous improvement and prevent customer escapes. Full article

Mesozoic tree ferns related to the extant Loxsomopsis, a relict and taxonomically isolated genus endemic to tropical South America, are exceedingly rare, making it difficult to trace its origin and evolution. Here, we describe a mid-Cretaceous fossil attributable to Loxsomopsis, Loxsomopsis minor sp. nov., based on two fertile pinnules preserved in Myanmar amber. The new species is assigned to the extant genus based on a suite of diagnostic features, including pinnate fertile segments with short lobes, marginal paraphysate sori, narrowly cyathiform to urceolate indusia, columnar and exserted receptacles, oblique sporangial annuli, and trilete spores. This discovery extends the fossil record of Loxsomopsis back to the Albian–Cenomanian and provides new evidence that the lineage—now restricted to tropical South America—was once more widespread. The occurrence of Loxsomopsis in the Myanmar amber biota is consistent with a Gondwanan affinity for this mid-Cretaceous forest ecosystem and suggests that the genus may have likely originated prior to the breakup of Pangaea. Full article

The coordinated development of achenes and receptacles in strawberry is critical for seed dispersal and fruit quality, yet the underlying molecular mechanisms remain poorly characterized. Utilizing RNA-seq analysis during the ripening transition stage, we identified pronounced transcriptomic divergence between achenes and receptacles, with receptacles exhibiting more dynamic gene expression shifts. Intriguingly, a substantial subset of differentially expressed genes (DEGs) displayed antagonistic expression patterns between these tissues, including the cytokinin degradation gene cytokinin oxidase/dehydrogenase 1 (FvCKX1), which was highly expressed in both tissues but with opposing temporal trends. Functional interrogation via transient silencing and overexpression revealed a tissue-specific regulatory role for FvCKX1. RNA interference (RNAi) suppression of FvCKX1 significantly enhanced receptacle expansion but delayed achene maturation, whereas overexpression inhibited receptacle growth while accelerating achene ripening. Abscisic acid (ABA), which positively regulates fruit enlargement, was elevated in FvCKX1 RNAi receptacles and notably reduced in overexpression fruits, indicating that FvCKX1 might negatively modulate ABA synthesis during strawberry fruit development. Our results demonstrate that FvCKX1 may function as a key regulator mediating the coordinated development between achenes and receptacles in strawberry. Full article

MLO (Mildew Resistance Locus O) genes encode seven-transmembrane proteins that function as critical regulators of powdery mildew resistance and abiotic stress responses. Despite their established importance, the MLO gene family in Gossypium hirsutum L. has not been systematically investigated under salt stress conditions. Here, we performed genome-wide identification of 46 GhMLO members using Hidden Markov Model and BLAST searches based on the latest cotton genome assembly. Phylogenetic analysis classified these genes into four distinct subfamilies. Transmembrane topology and conserved domain analyses revealed that all GhMLO proteins contain typical MLO domains and transmembrane structures, maintaining high structural similarity with dicotyledonous model plants. Synteny analysis demonstrated that the expansion of the GhMLO family was primarily driven by segmental and tandem duplications. Integration of transcriptomic data from the COTTONOMICS database revealed tissue-specific expression patterns, with higher transcript abundance in receptacles, stems, and roots, but lower levels in stamens and petals. Salt, drought, and cold stress treatments induced upregulation of GhMLO family members, with most genes showing increased expression over time. RT-qPCR analysis validated that five candidate GhMLO genes were significantly upregulated under salt stress. In summary, this study provides a comprehensive genome-wide characterization of the GhMLO gene family, elucidating their phylogenetic relationships and expression dynamics, which establishes a theoretical basis for identifying key regulatory genes involved in abiotic stress responses and offers novel genetic resources for improving stress tolerance in cotton molecular breeding. Full article

The Meso-Papilionoideae clade comprises most papilionoid legumes and includes small clades with heterogeneous floral morphologies. Some species have a sessile ovary, while in others the gynoecium is elevated by a stalk called a stipe or gynophore. This study provides a qualitative and comparative morphological analysis of meso-papilionoid flowers, focusing on the anatomy, vascularization, and development of the ovary and gynophore. The objective is to unravel the ontogenic origin and anatomical nature of the gynophore in meso-papilionoid flowers. Floral buds at different developmental stages of seven meso-papilionoid species were examined using scanning electron microscopy and sectioned transversely and longitudinally for analysis under optical microscopy. The morphological variations in the examined flowers may represent evolutionary adaptations associated with their respective pollination syndromes. Ovary development follows a certain pattern among legumes, with limited variations, including the formation of a basal pedestal beneath the carpel suture in species bearing a gynophore. The gynophore is anatomically distinct from the ovary, exhibiting a stem-like nature, originating from the activity of an intercalary meristem on the basal pedestal of the ovary or receptacle. This qualitative anatomical approach represents a first step toward the homologation of gynophore types in Fabaceae, providing a basis for future quantitative and phylogenetic analyses. Full article

The construction industry faces challenges in estimating costs because the processes are time-consuming and involve a high likelihood of making errors. For instance, quantity take-offs are often inaccurate, and there is not a simple way to integrate data from Building Information Modeling (BIM) platforms and cost databases. This study introduces a framework that utilizes the Model Context Protocol (MCP) to ensure seamless integration between large language models (LLMs) and BIM models through Autodesk Revit in order to enable fully automated cost estimation workflows. The developed system combines an AI-powered MCP server with cost databases that are standard in the industry, such as the 2025 Craftsman National Building Cost Manual and the ZIP code-based location modifiers. This system enables LLMs to automatically obtain quantities from BIM models, match components to cost items, make regional changes, and make professional cost estimates. A case study of estimating the cost of an electrical system shows that the framework can reduce estimation time from 2.5–3.5 h (manual baseline) to 42.3 ± 3.7 s (n = 5 runs, warm start), representing a 98.6% efficiency gain, while being more accurate with respect to industry standards. The system processed 187 BIM elements in three component groups (receptacles, conduits, and panels). It automatically matched them to the right cost database items, used location-specific modifiers for ZIP code 01003, and made a full cost estimate of USD 13,945.81 with detailed breakdowns and a percent difference of %5.1 of the manual estimation. This research enhances automation in construction by developing a methodology for AI-BIM integration using standardized protocols, shows the practical application of AI in construction workflows, and provides empirical evidence of the advantages of automation in cost estimation processes. The results indicate that MCP-based AI integration presents a novel approach for construction automation, delivering improvements while applying professional standards of accuracy and availability. Full article

The global proliferation of plastics and their degradation into microplastics (<5 mm) have created a pervasive environmental crisis with severe ecological and human health consequences. Despite the exponential growth in microplastic research over the past decade, standardized protocols are still lacking. The absence of consistent sampling, analysis, and reporting methods limits data comparability, interoperability, and harmonization across studies. This study conducted a systematic bibliographic review of 355 peer-reviewed articles published between 2010 and 2022 that investigated microplastics in freshwater as well as marine water and sediment environments. The goal was to evaluate methodological consistency, sampling instruments, measurement units, reported characteristics, and data-sharing practices to identify pathways toward harmonized and FAIR (Findable, Accessible, Interoperable, and Reusable) microplastic data. Results show that 80.6% of studies focused on marine environments, 18% on freshwater, and 1.4% on both. This highlights persistent data gaps in freshwater systems, which function as key transport pathways for plastics to the ocean. Most studies targeted water (59%) rather than sediment (41%) and were mostly based on single-time sampling, limiting long-term analyses. Surface layers (<1 m) were predominantly sampled, while deeper layers remain understudied. Nets, particularly Manta, neuston, and plankton nets were the dominant tools for water sampling, whereas grabs, corers, and metallic receptacles were used for sediments. However, variations in mesh size and sampling depth introduce substantial biases in particle size recovery and reduce comparability across studies. The most common units were counts/volume for water and counts/g dry weight for sediments, but more than ten unit expressions were identified, complicating conversions. Only 35% of studies reported all four key microplastic characteristics (color, polymer type, shape, and size), and less than 20% made datasets publicly available. To advance harmonization, we recommend the adoption of consistent measurement units, mandatory reporting of key metadata, and wider implementation of open data practices aligned with the FAIR principles. These insights provide a foundation for developing robust monitoring strategies and evidence-based management frameworks. This is especially important for freshwater systems, where data remain scarce, and policy intervention is urgently needed. Full article

Morphological and molecular phylogenetic studies of Chlorencoelia in China revealed three novel species, which are described and illustrated as C. biguttulata, C. ellipsoidea, and C. sinensis. Chlorencoelia biguttulata is characterized by greyish green to blackish green hymenium surface, clavate tomentum hyphae on receptacle surface, and inequilateral ellipsoidal ascospores having two large guttules and 8.5–14.5 × 3.5–4.5 μm. Chlorencoelia ellipsoidea differs from the known species by distinctly stipitate apothecia with a dark green hymenium surface and ellipsoidal ascospores 7.2–9.1 × 3.5–4.2 μm. Chlorencoelia sinensis is characterized by substipitate apothecia, blackish receptacle surface, and fusoidal ascospores 9–13.7 × 2.2–3.5 μm. Sequence analyses of the ITS region supported recognition of them as separate species. All three new species occurred on rotten wood in montane forests. Morphological features for species delimitation and diversity of Chlorencoelia are discussed. An identification key to the known species of the genus is provided. Full article

Matricaria chamomilla and Tripleurospermum inodorum (syn. Matricaria inodora) are two closely related species in the Asteraceae family that are often mistaken for one another due to their similar appearance. However, they differ significantly in their chemical composition and biological activities. This study offers comparative characterisation through microscopy, phytochemical profiling, and biological assays. Microscopic observations revealed distinct morphological differences in the structure of the receptacle and the size of the pollen grains between the two species. Total phenol and flavonoid contents were quantified using spectrophotometry, while essential oils were extracted through hydrodistillation and analysed by gas chromatography–mass spectrometry (GC-MS). M. chamomilla was found to have a higher phenol content (20.48 mg GAE/g DW), whereas T. inodorum showed a greater flavonoid concentration (15.93 mg RE/g DW). The essential oils from each species displayed different chemical composition: M. chamomilla was dominated by bisabolol oxides and chamazulene, while T. inodorum primarily contained β-farnesene and cis-lachnophyllum ester. The antioxidant activity of both species was evaluated using the DPPH assay and found to be moderate compared to standard antioxidants, such as ascorbic acid (IC₅₀ < 5 µg/mL). The IC₅₀ values for M. chamomilla ranged from 17.7 to 21.5 µg/mL, while for T. inodorum, they ranged from 8.4 to 10.2 µg/mL. In antimicrobial tests, the essential oil of T. inodorum inhibited both Staphylococcus aureus and Candida albicans, while M. chamomilla was only active against C. albicans. These findings highlight important morphological and chemical markers that differentiate the two species and affirm T. inodorum as a promising source of bioactive compounds. Full article