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23 pages, 10836 KiB  
Article
Potential Utilization of End-of-Life Vehicle Carpet Waste in Subfloor Mortars: Incorporation into Portland Cement Matrices
by Núbia dos Santos Coimbra, Ângela de Moura Ferreira Danilevicz, Daniel Tregnago Pagnussat and Thiago Gonçalves Fernandes
Materials 2025, 18(15), 3680; https://doi.org/10.3390/ma18153680 (registering DOI) - 5 Aug 2025
Abstract
The growing need to improve the management of end-of-life vehicle (ELV) waste and mitigate its environmental impact is a global concern. One promising approach to enhancing the recyclability of these vehicles is leveraging synergies between the automotive and construction industries as part of [...] Read more.
The growing need to improve the management of end-of-life vehicle (ELV) waste and mitigate its environmental impact is a global concern. One promising approach to enhancing the recyclability of these vehicles is leveraging synergies between the automotive and construction industries as part of a circular economy strategy. In this context, ELV waste emerges as a valuable source of secondary raw materials, enabling the development of sustainable innovations that capitalize on its physical and mechanical properties. This paper aims to develop and evaluate construction industry composites incorporating waste from ELV carpets, with a focus on maintaining or enhancing performance compared to conventional materials. To achieve this, an experimental program was designed to assess cementitious composites, specifically subfloor mortars, incorporating automotive carpet waste (ACW). The results demonstrate that, beyond the physical and mechanical properties of the developed composites, the dynamic stiffness significantly improved across all tested waste incorporation levels. This finding highlights the potential of these composites as an alternative material for impact noise insulation in flooring systems. From an academic perspective, this research advances knowledge on the application of ACW in cement-based composites for construction. In terms of managerial contributions, two key market opportunities emerge: (1) the commercial exploitation of composites produced with ELV carpet waste and (2) the development of a network of environmental service providers to ensure a stable waste supply chain for innovative and sustainable products. Both strategies contribute to reducing landfill disposal and mitigating the environmental impact of ELV waste, reinforcing the principles of the circular economy. Full article
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15 pages, 1507 KiB  
Article
Determination of Fumonisins B1 and B2 in Food Matrices: Optimisation of a Liquid Chromatographic Method with Fluorescence Detection
by Óscar Cebadero-Domínguez, Santiago Ruiz-Moyano, Alberto Martín and Elisabet Martín-Tornero
Toxins 2025, 17(8), 391; https://doi.org/10.3390/toxins17080391 - 5 Aug 2025
Abstract
Fumonisins, primarily produced by Fusarium spp. and Aspergillus section nigri, are common contaminants in maize, cereal grains, and other processed and derived products, representing a significant risk to food safety and public health. This study presents the development and optimisation of a [...] Read more.
Fumonisins, primarily produced by Fusarium spp. and Aspergillus section nigri, are common contaminants in maize, cereal grains, and other processed and derived products, representing a significant risk to food safety and public health. This study presents the development and optimisation of a high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) for the quantification of fumonisin B1 (FB1) and B2 (FB2) in various food matrices. In contrast with conventional protocols employing potassium phosphate buffers as the mobile phase, the proposed method utilises formic acid, offering enhanced compatibility with liquid chromatography systems. An automated online precolumn derivatisation with o-phthaldialdehyde (OPA) was optimised through experimental design and response surface methodology, enabling baseline separation of FB1 and FB2 derivatives in less than 20 min. The method demonstrated high sensitivity, with limits of detection of 0.006 µg mL−1 for FB1 and 0.012 µg mL−1 for FB2, and excellent repeatability (intraday RSD values of 0.85% and 0.83%, respectively). Several solid-phase extraction (SPE) strategies were evaluated to enhance sample clean-up using a variety of food samples, including dried figs, raisins, dates, corn, cornmeal, wheat flour, and rice. FumoniStar Inmunoaffinity columns were the only clean-up method that provided optimal recoveries (70–120%) across all tested food matrices. However, the MultiSep™ 211 column yielded good recoveries for both fumonisins in dried figs and raisins. Additionally, the C18 cartridge achieved acceptable recoveries for both fumonisins in dried figs and wheat flour. Full article
(This article belongs to the Section Mycotoxins)
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9 pages, 781 KiB  
Article
Absence of Sulfur Fertilization at Establishment in Urochloa brizantha Cultivars
by Carlos Eduardo Avelino Cabral, Luis Carlos Oliveira Borges, Anna Cláudia Cardoso Paimel, Eildson Souza de Oliveira Silva, Izabela Aline Gomes da Silva, Camila Fernandes Domingues Duarte, Lucas Gimenes Mota, Anne Caroline Dallabrida Avelino and Carla Heloisa Avelino Cabral
Grasses 2025, 4(3), 31; https://doi.org/10.3390/grasses4030031 - 5 Aug 2025
Abstract
Sulfur-containing fertilizers increase production costs, which leads to low utilization of this nutrient. Thus, evaluating how the absence of sulfur influences the early development of Urochloa brizantha is essential. Study was conducted in a greenhouse at the Federal University of Rondonópolis in a [...] Read more.
Sulfur-containing fertilizers increase production costs, which leads to low utilization of this nutrient. Thus, evaluating how the absence of sulfur influences the early development of Urochloa brizantha is essential. Study was conducted in a greenhouse at the Federal University of Rondonópolis in a completely randomized design, with six treatments in a 3 × 2 factorial scheme, and eight replications. Three cultivars of U. brizantha (Marandu, Xaraés and Piatã) were evaluated under two fertilization strategies: with or without sulfur fertilization. Sufur presence increased the number of leaves and forage mass, in which cultivar Xaraés presented the greatest means. Piatã was the cultivar most sensitive to sulfur deficiency at establishment, which reduced forage mass, number of leaves and number of tillers by 42%, 32%, and 45%, respectively. Despite these differences between cultivars, sulfur efficiently increased the forage yield. Sulfur fertilization increased the concentrations of nutrients in the plants without significantly affecting the uptake of nitrogen, phosphorus, potassium, calcium and magnesium. Sulfur omission resulted in increased phosphorus uptake in all grass. In contrast, Marandu grass exhibited the greatest reduction in sulfur uptake. Therefore, the use of sulfur in the fertilization of grasses is recommended, it is important to evaluate the responses of each cultivar to better adjust the fertilization management. Full article
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13 pages, 1841 KiB  
Article
Valorizing Biomass Waste: Hydrothermal Carbonization and Chemical Activation for Activated Carbon Production
by Fidel Vallejo, Diana Yánez, Luis Díaz-Robles, Marcelo Oyaneder, Serguei Alejandro-Martín, Rasa Zalakeviciute and Tamara Romero
Biomass 2025, 5(3), 45; https://doi.org/10.3390/biomass5030045 - 5 Aug 2025
Abstract
This study optimizes the production of activated carbons from hydrothermally carbonized (HTC) biomass using potassium hydroxide (KOH) and phosphoric acid (H3PO4) as activating agents. A 23 factorial experimental design evaluated the effects of agent-to-precursor ratio, dry impregnation time, [...] Read more.
This study optimizes the production of activated carbons from hydrothermally carbonized (HTC) biomass using potassium hydroxide (KOH) and phosphoric acid (H3PO4) as activating agents. A 23 factorial experimental design evaluated the effects of agent-to-precursor ratio, dry impregnation time, and activation duration on mass yield and iodine adsorption capacity. KOH-activated carbons achieved superior iodine numbers (up to 1289 mg/g) but lower mass yields (18–35%), reflecting enhanced porosity at the cost of material loss. Conversely, H3PO4 activation yielded higher mass retention (up to 54.86%) with moderate iodine numbers (up to 1117.3 mg/g), balancing porosity and yield. HTC pretreatment at 190 °C reduced the ash content, thereby enhancing the stability of hydrochar. These findings highlight the trade-offs between adsorption performance and process efficiency, with KOH suited for high-porosity applications (e.g., water purification) and H3PO4 for industrial scalability. The study advances biomass waste valorization, aligning with circular economy principles and offering sustainable solutions for environmental and industrial applications, such as water purification and energy storage. Full article
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18 pages, 309 KiB  
Review
Geographical Indication Labels for Food Products: A Literature Review
by Bella Crespo-Moncada, Guiomar Denisse Posada-Izquierdo, Jorge Velásquez-Rivera, John Molina-Villamar and Rosa María García-Gimeno
Encyclopedia 2025, 5(3), 115; https://doi.org/10.3390/encyclopedia5030115 - 5 Aug 2025
Abstract
Geographical Indication labels are an increasingly popular alternative among producers and governments seeking to protect the products and producers of their countries. This trend has grown due to the opening of markets and consumers’ increasing desire to know the origin of the products [...] Read more.
Geographical Indication labels are an increasingly popular alternative among producers and governments seeking to protect the products and producers of their countries. This trend has grown due to the opening of markets and consumers’ increasing desire to know the origin of the products they purchase. A bibliographic review was conducted, including studies on the feasibility of applying quality labels, the associated challenges, and examples. This review identifies the processes involved in obtaining Designation of Origin and presents a positive perspective on their application. It was concluded that obtaining a differentiated quality label can improve production and quality of life, provided that it is the result of an evaluation of the area’s context and the main actors in production chains. Full article
(This article belongs to the Section Chemistry)
13 pages, 1859 KiB  
Article
Suspension Fertilizers Based on Waste Organic Matter from Peanut Oil Extraction By-Products
by Sainan Xiang, Baoshen Li and Yang Lyu
Agronomy 2025, 15(8), 1885; https://doi.org/10.3390/agronomy15081885 - 5 Aug 2025
Abstract
The use of chemical fertilizers has significantly increased crop yields but has also led to soil problems such as nutrient imbalance and salinization. In response, organic fertilizers have emerged as a crucial component for sustainable agricultural development. This study was designed to develop [...] Read more.
The use of chemical fertilizers has significantly increased crop yields but has also led to soil problems such as nutrient imbalance and salinization. In response, organic fertilizers have emerged as a crucial component for sustainable agricultural development. This study was designed to develop an easily applicable organic suspension fertilizer using peanut bran, the primary by-product of peanut oil extraction, as the main raw material. Fourier-transform infrared (FTIR) analysis revealed that 80 °C is the optimal heating temperature for forming a stable peanut-bran suspension. A comprehensive experimental investigation was conducted to evaluate the effects of different peanut bran addition levels, stabilizers, emulsifiers, and suspending agents on the stability of suspension fertilizers. The results identified the optimal suspension fertilizer formulation as comprising 20% peanut bran, 0.5% sodium bentonite, 0.1% monoglyceride, 0.2% sucrose ester, 0.02% carrageenan, and 0.3% xanthan gum. This formulation ensures good stability and fluidity of the suspension fertilizer while maintaining a low cost of 0.134 USD·kg−1. The findings provide a scalable technological framework for valorizing agro-industrial waste into high-performance organic fertilizers. Full article
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180 KiB  
Proceeding Paper
Design of Automatic Generation Platform for Agricultural Robot
by Zhaowei Wang, Yurong Wang and Fangji Zhang
Eng. Proc. 2025, 98(1), 45; https://doi.org/10.3390/engproc2025098045 - 4 Aug 2025
Abstract
The design of robots is highly dependent on their applications. For agricultural robots, terrain, weather, and crop diversity need to be considered, and work efficiency, cost, and reliability must be evaluated. These factors are important to determine the design of agricultural robots. In [...] Read more.
The design of robots is highly dependent on their applications. For agricultural robots, terrain, weather, and crop diversity need to be considered, and work efficiency, cost, and reliability must be evaluated. These factors are important to determine the design of agricultural robots. In this study, we identified the constraint factors of agricultural robots from the perspectives of navigation, movement, control, cost, and reliability. The orthogonal defect classification (ODC) method was used to classify and grade these factors and explore the relationships among these factors. Based on the results, the design rules of agricultural robots were created, and an automatic production knowledge base of agricultural robot design was constructed. The results contribute to the automatic generation of the design framework of agricultural robots under specific environments to effectively improve the design level and quality of agricultural robots and popularize agricultural robots. Full article
19 pages, 14190 KiB  
Article
A Comprehensive Evaluation Method for Cement Slurry Systems to Enhance Zonal Isolation: A Case Study in Shale Oil Well Cementing
by Xiaoqing Zheng, Weitao Song, Xiutian Yang, Jian Liu, Tao Jiang, Xuning Wu and Xin Liu
Energies 2025, 18(15), 4138; https://doi.org/10.3390/en18154138 - 4 Aug 2025
Abstract
Due to post-cementing hydraulic fracturing and other operational stresses, inadequate mechanical properties or suboptimal design of the cement sheath can lead to tensile failure and microcrack development, compromising both hydrocarbon recovery and well integrity. In this study, three field-deployed cement slurry systems were [...] Read more.
Due to post-cementing hydraulic fracturing and other operational stresses, inadequate mechanical properties or suboptimal design of the cement sheath can lead to tensile failure and microcrack development, compromising both hydrocarbon recovery and well integrity. In this study, three field-deployed cement slurry systems were compared on the basis of their basic mechanical properties such as compressive and tensile strength. Laboratory-scale physical simulations of hydraulic fracturing during shale oil production were conducted, using dynamic permeability as a quantitative indicator of integrity loss. The experimental results show that evaluating only basic mechanical properties is insufficient for cement slurry system design. A more comprehensive mechanical assessment is re-quired. Incorporation of an expansive agent into the cement slurry system can alleviate the damage caused by the microannulus to the interfacial sealing performance of the cement sheath, while adding a toughening agent can alleviate the damage caused by tensile cracks to the sealing performance of the cement sheath matrix. Through this research, a microexpansive and toughened cement slurry system, modified with both expansive and toughening agents, was optimized. The expansive agent and toughening agent can significantly enhance the shear strength, the flexural strength, and the interfacial hydraulic isolation strength of cement stone. Moreover, the expansion agents mitigate the detrimental effects of microannulus generation on the interfacial sealing, while the toughening agents alleviate the damage caused by tensile cracking to the bulk sealing performance of the cement sheath matrix. This system has been successfully implemented in over 100 wells in the GL block of Daqing Oilfield. Field application results show that the proportion of high-quality well sections in the horizontal section reached 88.63%, indicating the system’s high performance in enhancing zonal isolation and cementing quality. Full article
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25 pages, 30553 KiB  
Article
Optimizing Multi-Cluster Fracture Propagation and Mitigating Interference Through Advanced Non-Uniform Perforation Design in Shale Gas Horizontal Wells
by Guo Wen, Wentao Zhao, Hongjiang Zou, Yongbin Huang, Yanchi Liu, Yulong Liu, Zhongcong Zhao and Chenyang Wang
Processes 2025, 13(8), 2461; https://doi.org/10.3390/pr13082461 - 4 Aug 2025
Abstract
The persistent challenge of fracture-driven interference (FDI) during large-scale hydraulic fracturing in the southern Sichuan Basin has severely compromised shale gas productivity, while the existing research has inadequately addressed both FDI risk reductions and the optimization of reservoir stimulation. To bridge this gap, [...] Read more.
The persistent challenge of fracture-driven interference (FDI) during large-scale hydraulic fracturing in the southern Sichuan Basin has severely compromised shale gas productivity, while the existing research has inadequately addressed both FDI risk reductions and the optimization of reservoir stimulation. To bridge this gap, this study developed a mechanistic model of the competitive multi-cluster fracture propagation under non-uniform perforation conditions and established a perforation-based design methodology for the mitigation of horizontal well interference. The results demonstrate that spindle-shaped perforations enhance the uniformity of fracture propagation by 20.3% and 35.1% compared to that under uniform and trapezoidal perforations, respectively, with the perforation quantity (48) and diameter (10 mm) identified as the dominant control parameters for balancing multi-cluster growth. Through a systematic evaluation of the fracture communication mechanisms, three distinct inter-well types of FDI were identified: Type I (natural fracture–stress anisotropy synergy), Type II (natural-fracture-dominated), and Type III (stress-anisotropy-dominated). To mitigate these, customized perforation schemes coupled with geometry-optimized fracture layouts were developed. The surveillance data for the offset well show that the pressure interference decreased from 14.95 MPa and 6.23 MPa before its application to 0.7 MPa and 0 MPa, achieving an approximately 95.3% reduction in the pressure interference in the application wells. The expansion morphology of the inter-well fractures confirmed effective fluid redistribution across clusters and containment of the overextension of planar fractures, demonstrating this methodology’s dual capability to enhance the effectiveness of stimulation while resolving FDI challenges in deep shale reservoirs, thereby advancing both productivity and operational sustainability in complex fracturing operations. Full article
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22 pages, 858 KiB  
Article
Dual-Pathway Effects of Product and Technological Attributes on Consumer Engagement in Augmented Reality Advertising
by Peng He and Jing Zhang
J. Theor. Appl. Electron. Commer. Res. 2025, 20(3), 196; https://doi.org/10.3390/jtaer20030196 - 4 Aug 2025
Abstract
As augmented reality (AR) advertising becomes increasingly prevalent across digital platforms, understanding how its unique features influence consumer responses is critical for both theory and practice. Based on the elaboration likelihood model (ELM), this study develops and validates a dual-dimension content–dual-route processing model [...] Read more.
As augmented reality (AR) advertising becomes increasingly prevalent across digital platforms, understanding how its unique features influence consumer responses is critical for both theory and practice. Based on the elaboration likelihood model (ELM), this study develops and validates a dual-dimension content–dual-route processing model to investigate how different features of AR advertising influence consumer engagement. Specifically, it examines how product-related attributes (attractiveness, informativeness) and technology-related attributes (interactivity, augmentation) shape attitudes toward the ad and purchase intentions through cognitive (information credibility) and affective (enjoyment) pathways. Using data from an online survey (N = 299), the study applies partial least squares structural equation modeling (PLS-SEM) to test the proposed model. The results show that informativeness and augmentation significantly enhance information credibility, while attractiveness primarily influences emotional responses. Interactivity and augmentation positively influence cognitive and affective responses. Mediation analysis confirms the simultaneous activation of central and peripheral processing routes, with flow experience emerging as a significant moderator in selected pathways. By introducing a structured framework for AR advertising content, this study extends the applicability of the ELM in immersive media contexts. It underscores the combined impact of rational evaluation and emotional engagement in shaping consumer behavior and offers practical insights for designing effective AR advertising strategies. Full article
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37 pages, 10560 KiB  
Article
Optimizing Building Performance with Dynamic Photovoltaic Shading Systems: A Comparative Analysis of Six Adaptive Designs
by Roshanak Roshan Kharrat, Giuseppe Perfetto, Roberta Ingaramo and Guglielmina Mutani
Smart Cities 2025, 8(4), 127; https://doi.org/10.3390/smartcities8040127 - 3 Aug 2025
Abstract
Dynamic and Adaptive solar systems demonstrate a greater potential to enhance the satisfaction of occupants, in terms of indoor environment quality and the energy efficiency of the buildings, than conventional shading solutions. This study has evaluated Dynamic and Adaptive Photovoltaic Shading Systems (DAPVSSs) [...] Read more.
Dynamic and Adaptive solar systems demonstrate a greater potential to enhance the satisfaction of occupants, in terms of indoor environment quality and the energy efficiency of the buildings, than conventional shading solutions. This study has evaluated Dynamic and Adaptive Photovoltaic Shading Systems (DAPVSSs) through a comprehensive analysis of six shading designs in which their energy production and the comfort of occupants were considered. Energy generation, thermal comfort, daylight, and glare control have been assessed in this study, considering multiple orientations throughout the seasons, and a variety of tools, such as Rhino 6.0, Grasshopper, ClimateStudio 2.1, and Ladybug, have been exploited for these purposes. The results showed that the prototypes that were geometrically more complex, designs 5 and 6 in particular, had approximately 485 kWh higher energy production and energy savings for cooling and 48% better glare control than the other simplified configurations while maintaining the minimum daylight as the threshold (min DF: 2%) due to adaptive and control methodologies. Design 6 demonstrated optimal balanced performance for all the aforementioned criteria, achieving 587 kWh/year energy production while maintaining the daylight factor within the 2.1–2.9% optimal range and ensuring visual comfort compliance during 94% of occupied hours. This research has established a framework that can be used to make well-informed design decisions that could balance energy production, occupants’ wellbeing, and architectural integration, while advancing sustainable building envelope technologies. Full article
(This article belongs to the Topic Sustainable Building Development and Promotion)
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21 pages, 3108 KiB  
Article
Dynamic Expectation–Satisfaction Relationship in Sustainable Experiences with Product: A Comparative Study of Durable Goods, FMCG, and Digital Products
by Zhenhua Wu, Kenta Ono and Yuting Wu
Sustainability 2025, 17(15), 7045; https://doi.org/10.3390/su17157045 - 3 Aug 2025
Viewed by 64
Abstract
This study adopts a dynamic Expectancy–Disconfirmation framework to investigate the evolving nature of user satisfaction across three product categories: durable goods, fast-moving consumer goods (FMCG), and digital products. A 25-day longitudinal experiment involving 128 participants was conducted, during which users engaged with their [...] Read more.
This study adopts a dynamic Expectancy–Disconfirmation framework to investigate the evolving nature of user satisfaction across three product categories: durable goods, fast-moving consumer goods (FMCG), and digital products. A 25-day longitudinal experiment involving 128 participants was conducted, during which users engaged with their most recently purchased products and provided repeated subjective evaluations over time. The findings reveal dynamic changes in the influence of expectations and perceived performance on satisfaction throughout the product usage cycle. For durable goods and FMCG, both expectations and perceived performance gradually declined, accompanied by a weakening effect of expectations on satisfaction. In contrast, digital products exhibited greater volatility, lacking a stable experiential baseline and resulting in greater fluctuations in satisfaction trajectories. Moreover, external contextual and emotional factors were found to play a more significant role in shaping satisfaction with physical products, beyond the scope of the traditional expectancy–performance model. These insights offer theoretical and managerial implications for sustainable product and experience design. In particular, they highlight the importance of implementing experience-stabilizing strategies in digital consumption contexts to support user well-being and enhance continuous product utilization, thereby maximizing product potential and reducing waste. Full article
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21 pages, 4467 KiB  
Review
Structuring the Future of Cultured Meat: Hybrid Gel-Based Scaffolds for Edibility and Functionality
by Sun Mi Zo, Ankur Sood, So Yeon Won, Soon Mo Choi and Sung Soo Han
Gels 2025, 11(8), 610; https://doi.org/10.3390/gels11080610 - 3 Aug 2025
Viewed by 44
Abstract
Cultured meat is emerging as a sustainable alternative to conventional animal agriculture, with scaffolds playing a central role in supporting cellular attachment, growth, and tissue maturation. This review focuses on the development of gel-based hybrid biomaterials that meet the dual requirements of biocompatibility [...] Read more.
Cultured meat is emerging as a sustainable alternative to conventional animal agriculture, with scaffolds playing a central role in supporting cellular attachment, growth, and tissue maturation. This review focuses on the development of gel-based hybrid biomaterials that meet the dual requirements of biocompatibility and food safety. We explore recent advances in the use of naturally derived gel-forming polymers such as gelatin, chitosan, cellulose, alginate, and plant-based proteins as the structural backbone for edible scaffolds. Particular attention is given to the integration of food-grade functional additives into hydrogel-based scaffolds. These include nanocellulose, dietary fibers, modified starches, polyphenols, and enzymatic crosslinkers such as transglutaminase, which enhance mechanical stability, rheological properties, and cell-guidance capabilities. Rather than focusing on fabrication methods or individual case studies, this review emphasizes the material-centric design strategies for building scalable, printable, and digestible gel scaffolds suitable for cultured meat production. By systemically evaluating the role of each component in structural reinforcement and biological interaction, this work provides a comprehensive frame work for designing next-generation edible scaffold systems. Nonetheless, the field continues to face challenges, including structural optimization, regulatory validation, and scale-up, which are critical for future implementation. Ultimately, hybrid gel-based scaffolds are positioned as a foundational technology for advancing the functionality, manufacturability, and consumer readiness of cultured meat products, distinguishing this work from previous reviews. Unlike previous reviews that have focused primarily on fabrication techniques or tissue engineering applications, this review provides a uniquely food-centric perspective by systematically evaluating the compositional design of hybrid hydrogel-based scaffolds with edibility, scalability, and consumer acceptance in mind. Through a comparative analysis of food-safe additives and naturally derived biopolymers, this review establishes a framework that bridges biomaterials science and food engineering to advance the practical realization of cultured meat products. Full article
(This article belongs to the Special Issue Food Hydrocolloids and Hydrogels: Rheology and Texture Analysis)
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20 pages, 4784 KiB  
Article
Resilient by Design: Environmental Stress Promotes Biofilm Formation and Multi-Resistance in Poultry-Associated Salmonella
by Gabriel I. Krüger, Francisca Urbina, Coral Pardo-Esté, Valentina Salinas, Javiera Álvarez, Nicolás Avilés, Ana Oviedo, Catalina Kusch, Valentina Pavez, Rolando Vernal, Mario Tello, Luis Alvarez-Thon, Juan Castro-Severyn, Francisco Remonsellez, Alejandro Hidalgo and Claudia P. Saavedra
Microorganisms 2025, 13(8), 1812; https://doi.org/10.3390/microorganisms13081812 - 3 Aug 2025
Viewed by 59
Abstract
Salmonella is one of the main causes of food-borne illness worldwide. In most cases, Salmonella contamination can be traced back to food processing plants and/or to cross-contamination during food preparation. To avoid food-borne diseases, food processing plants use sanitizers and biocidal to reduce [...] Read more.
Salmonella is one of the main causes of food-borne illness worldwide. In most cases, Salmonella contamination can be traced back to food processing plants and/or to cross-contamination during food preparation. To avoid food-borne diseases, food processing plants use sanitizers and biocidal to reduce bacterial contaminants below acceptable levels. Despite these preventive actions, Salmonella can survive and consequently affect human health. This study investigates the adaptive capacity of the main Salmonella enterica serotypes isolated from the poultry production line, focusing on their replication, antimicrobial resistance, and biofilm formation under stressors such as acidic conditions, oxidative environment, and high osmolarity. Using growth curve analysis, crystal violet staining, and microscopy, we assessed replication, biofilm formation, and antimicrobial resistance under acidic, oxidative, and osmotic stress conditions. Disinfectant tolerance was evaluated by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of sodium hypochlorite. The antibiotic resistance was assessed using the Kirby–Bauer method. The results indicate that, in general, acidic and osmotic stress reduce the growth of Salmonella. However, no significant differences were observed specifically for serotypes Infantis, Heidelberg, and Corvallis. The S. Infantis isolates were the strongest biofilm producers and showed the highest prevalence of multidrug resistance (71%). Interestingly, S. Infantis forming biofilms required up to 8-fold higher concentrations of sodium hypochlorite for eradication. Furthermore, osmotic and oxidative stress significantly induced biofilm production in industrial S. Infantis isolates compared to a reference strain. Understanding how Salmonella responds to industrial stressors is vital for designing strategies to control the proliferation of these highly adapted, multi-resistant pathogens. Full article
(This article belongs to the Section Biofilm)
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22 pages, 3304 KiB  
Article
The Mechanism by Which Colour Patch Characteristics Influence the Visual Landscape Quality of Rhododendron simsii Landscape Recreational Forests
by Yan Liu, Juyang Liao, Yaqi Huang, Qiaoyun Li, Linshi Wu, Xinyu Yi, Ling Wang and Chan Chen
Horticulturae 2025, 11(8), 898; https://doi.org/10.3390/horticulturae11080898 (registering DOI) - 3 Aug 2025
Viewed by 56
Abstract
Landscape quality and the productivity of Rhododendron simsii are directly related to the maintenance of ecological functions in the alpine region. The specific relationship between the spatial pattern of colour patches and the visual quality of R. simsii landscape recreational forests has been [...] Read more.
Landscape quality and the productivity of Rhododendron simsii are directly related to the maintenance of ecological functions in the alpine region. The specific relationship between the spatial pattern of colour patches and the visual quality of R. simsii landscape recreational forests has been insufficiently explored. In this study, we constructed a model of the relationship between landscape colour patches and the aesthetic value of such a forest, analysing the key factors driving changes in its landscape quality. A total of 1549 participants were asked to assess 16 groups of landscape photographs. The results showed that variations in perceived aesthetic quality were stimulated by colour patch dynamics and spatial heterogeneity. Utilising structural equation modelling (SEM), we identified key indicators synergistically influencing aesthetic quality, including the area percentage, shape, and distribution of colour patches, which demonstrated strong explanatory power (R2 = 0.83). The SEM also revealed that the red patch area, mean perimeter area ratio, and separation index are critical latent variables with standardised coefficients of 0.54, 0.65, and 0.62, respectively. These findings provide actionable design strategies: (1) optimising chromatic contrast through high-saturation patches, (2) controlling geometric complexity, and (3) improving spatial coherence. These results advance the theoretical framework for landscape aesthetic evaluation and offer practical guidance for landscape recreational forest management. Full article
(This article belongs to the Section Floriculture, Nursery and Landscape, and Turf)
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