Search Results (214)

Insects underpin key ecosystem services. Yet tree-associated insect communities remain comparatively poorly documented, particularly in temperate forests. This study aimed to characterize the diversity and abundance of insect assemblages associated with the predominantly insect-pollinated forest tree Tilia cordata Mill. in protected areas in Lithuania, and to assess the occurrence of known and putative pollinator groups within these assemblages. We quantified insect assemblages associated with Tilia cordata using two sampling methods but did not directly measure pollination effectiveness (e.g., pollen loads, visitation rates to flowers, or fruit/seed set). Consequently, our inferences refer to the presence and composition of potential pollinators rather than demonstrated pollination function or realized pollination services. Fieldwork was conducted over two years in six protected T. cordata sites in Lithuania using two complementary sampling methods: net sampling and sticky traps. Sampling was structured into three observation periods corresponding to T. cordata phenology: pre-flowering (I), flowering (II) and post-flowering (III). In total, 207 insect taxa from 15 orders were recorded by net sampling and 86 taxa from 11 orders by sticky traps. Net sampling showed significantly higher diversity (Shannon H = 3.81) than sticky traps (H = 2.10). Hemiptera, Coleoptera and Diptera were the most common groups, and most taxa occurred at low to moderate abundances, with only a few species showing local dominance in specific periods or sites. Taxa documented in the literature as significant pollinators were consistently present but at low relative abundances (about 5–10% in total). Insect assemblage composition and species proportions varied among phenological periods and between years, with no clear, consistent peak in overall insect abundance or diversity associated specifically with the T. cordata flowering phase. These findings indicate that T. cordata stands in protected areas harbor diverse insect assemblages typical of temperate deciduous and mixed forest habitats and include a broad spectrum of non-bees and other potential pollinators. Therefore, we did not detect a distinct peak in insect abundance or species richness during the T. cordata flowering period, indicating that flowering did not coincide with a pronounced maximum in pollinator-related insect activity. However, the quantitative patterns observed suggest that, in this context, T. cordata provides only modest support for pollinator guilds, and its role is better interpreted as one component of wider forest insect diversity rather than as a primary driver of pollination services. Full article

Summer abiotic stresses typical of Mediterranean agro-environments, now exacerbated by climate change, reduce floral resource availability and further compromise the survival of pollinators already threatened in the so-called Anthropocene. The aim of this study was to evaluate several summer-blooming wildflower species, collected from ecologically disturbed and diversified habitats, in order to assess their ecological role in attracting pollinators within agroecosystems. The primary dormancy typical of wild species seeds was partially overcome through appropriate pre-sowing seed treatments, while secondary dormancy was reduced by soil rolling after sowing. Soil rolling proved particularly beneficial for species with very small seeds, highlighting the importance of adequate seed–soil contact for successful establishment. All tested species exhibited summer flowering between May and July, with some flowering later in the season, and showed high attractiveness to pollinators both in terms of abundance and taxonomic diversity. However, this ecosystem service declined significantly in the second year, although certain species demonstrated a strong capacity to persist and to maintain satisfactory pollinator attractiveness over time. In conclusion, while the experiment revealed several critical aspects, it also provides encouraging prospects for further research aimed at enhancing pollinator survival in agroecosystems that are severely threatened by pollinator decline. Full article

Bats (Chiroptera) provide critical ecosystem services, including pest suppression, pollination, and seed dispersal. Understanding their dietary ecology is essential for conservation management yet has historically been constrained by methodological limitations. This review synthesizes advances in bat dietary analysis over the past several decades, from traditional morphological and stable isotope approaches to the revolutionary DNA metabarcoding techniques that now dominate the field. We systematically evaluate the strengths and limitations of each methodological approach and examine how molecular methods have transformed our understanding of bat trophic ecology. Research progress across major feeding guilds—insectivorous, frugivorous, nectarivorous, carnivorous, and sanguivorous bats—is examined, with emphasis on recent discoveries enabled by molecular techniques. We discuss ecological and conservation applications, including ecosystem service quantification, food web construction, and responses to environmental change. Finally, we identify priority directions for future research, including long-read sequencing technologies, multi-method integration, reference database expansion, and One Health applications. This synthesis provides guidance for researchers selecting appropriate analytical approaches and highlights the critical role of dietary studies in bat conservation amid accelerating global change. Full article

Bats are a vital component of tropical forest ecosystems, providing essential services such as pollination, seed dispersal, and insect population regulation. Despite Malaysia’s high bat diversity, research has largely focused on lowland dipterocarp forests, leaving bat assemblages in specialized forest types poorly documented. To address this gap, a bat survey was conducted in Gunung Arong Forest Reserve, Peninsular Malaysia, with emphasis on heath and peat swamp habitats. Fourteen bat species representing Pteropodidae, Emballonuridae, Hipposideridae, Nycteridae, Rhinolophidae, and Vespertilionidae were recorded. The assemblage exhibited high diversity (H = 2.647) and evenness (e^H/S = 1.008), with low dominance (D = 0.0713), indicating a well-balanced community. Kerivoulinae accounted for 44% of captures, with Kerivoula intermedia as the most abundant species. Four Near Threatened species comprised 50% of all individuals captured, highlighting the conservation importance of these habitats. Notably, Arielulus societatis, endemic to Peninsular Malaysia, was recorded for the first time in Johor and represents the first documented occurrence in heath forests. The first mitochondrial COI sequence for A. societatis revealed a 5.44% genetic divergence from A. circumdatus. These findings underscore the ecological significance of heath and peat swamp forests and emphasize the need for targeted research and strengthened conservation efforts in Peninsular Malaysia. Full article

(1) Background: Bees of the genus Apis play a fundamental role in ecosystems thanks to their pollination activities and their long evolutionary history. This has resulted in species diversifying and spreading across Asia, Africa, and Europe. This review contextualises the genus within biogeographic and evolutionary frameworks, emphasising the importance of understanding the origins, adaptations, distribution and differences between species. (2) Methods: Recent studies on the biology, taxonomy and ecology of Apis species were analysed, including research on social behaviour, communication, genetics, morphology and environmental adaptations, as well as contributions using modern evolutionary and phylogeographic analytical methods. (3) Results: The gathered evidence shows that anthropogenic factors, including climate change, habitat loss, intensive agriculture, pollutants, competition with other bees and the spread of parasites and pathogens, significantly affect the stability of Apis populations and increase the vulnerability of wild species. (4) Conclusions: This review emphasises the importance of integrating ecological, genetic and management knowledge to develop effective conservation strategies that aim to reduce the impact of human activities and preserve the resilience of Apis species and the vital ecosystem services they provide. Full article

Apis mellifera L. is an important pollinator of both wild and domesticated crop plants, thus greatly contributing to plant biodiversity and commercial agriculture. However, in field conditions, honey bees remain exposed to different pesticides which ultimately affect colony health parameters and their associated ecological services. In the current study, the individual toxicities of sub-lethal doses of two distinct insecticides (bifenthrin and acetamiprid) belonging to different groups (pyrethroid and neonicotinoid) were assessed against the foraging activity, social interactions, and longevity of A. mellifera. The bees were exposed to individual doses of both insecticides via the dietary trophic route through contaminated pollen and nectar under natural field conditions. Sunflower crop (Hysun-33) was sown at nine different sites with an isolation distance of 3 km, and was treated with different doses (1/2, 1/4, 1/10, and 1/20 of the recommended field doses) of both insecticides. However, the untreated control crop plots were not subjected to any chemical treatments (bee colonies received no insecticide, and served as the baseline for making comparison). Twenty-seven bee colonies were introduced in these sites after seven days of treatment applications. Significant differences were observed in the foraging activity of A. mellifera (including bees going out from the hive, returning foragers, and those carrying pollens). The fecundity, adult longevity, and social behaviors like trophallaxis and antennation were significantly lower in bees exposed to higher individual insecticidal concentrations. However, the hatching duration, larval duration, and pupal duration were not affected by the tested insecticidal treatments. Overall, these findings demonstrate that the dietary trophic exposure of sub-lethal doses of insecticides compromised colony activities, which is indeed a matter of concern regarding the existing pesticide application methods in different agro-ecosystems. Such impacts may ultimately impair the survival of colonies, particularly when bees remain exposed to these chemicals over an extended period of time. Therefore, future studies must consider the pesticide application techniques and their application timing to mitigate the direct and indirect negative impacts of pesticides on pollinators. Full article

In northwestern Ecuador, where more than 90% of the original forest cover has been lost, it is unknown how soil chemistry influences bat diversity. This study evaluated bat diversity, non-herbaceous plant community structure, and soil nutrients in 30 plots distributed across crops on two farms separated by 32 km. Soil analyses revealed variations in organic matter and nutrients, identifying calcium, magnesium, zinc and iron as the most influential. A total of 1662 individuals of 24 non-herbaceous plant species and 193 individuals of 16 bat species were recorded, dominated by frugivorous and nectarivorous guilds. Generalized linear mixed models showed significant relationships between bat diversity indices and soil nutrients. These elements improve tree growth, fruiting, and flowering, which increases the quality and availability of food resources for bats. In return, these mammals provide key ecosystem services such as pollination, seed dispersal, and insect control. Our findings highlight that soil chemistry indirectly regulates bat communities by influencing vegetation structure and resource availability. This integrated approach underscores the importance of soil–plant–animal interactions in tropical agricultural landscapes, offering practical guidance. Full article

Chestnut is an important economic plant for ecology and farmers in mountainous areas in China. We conducted surveys and experiments to assess the economic value of the pollination service provided by insect pollinators for chestnuts via the Toolkit for Ecosystem Service Site-based Assessment (TESSA) in Bapeng Village, Wangmo County, Guizhou Province of China. We applied three methods presented by TESSA, the desk-based method, the field survey method, and the exclusion experiment, for the assessment. The resulting pollination values for the three methods are (1) CNY 3712.5∙ha⁻¹∙yr⁻¹ in the assessment site and an average of CNY 1386∙ha⁻¹∙yr⁻¹ with buffer zones included for the desk-based method; (2) CNY 3712.5∙ha⁻¹∙yr⁻¹ in the assessment site and an average of CNY 1124∙ha⁻¹∙yr⁻¹ with buffer zones included for the field survey method; and (3) CNY 4158∙ha⁻¹∙yr⁻¹ in the assessment site and an average of CNY 1485∙ha⁻¹∙yr⁻¹ with buffer zones included for the exclusion experiment method. The total value of chestnut pollination of the Bapeng Village ranges from CNY 311,943 yr⁻¹ to CNY 404,663 yr⁻¹. The chestnut pollination of the village is substantially insufficient. This could be the result of the decrease in diversity and uneven distribution of insect pollinators which, per se, are caused by the lack of larval hosts for those pollinators. Full article

The relationship between insect diversity and crop production has been of continuous scientific interest. Understanding insect community dynamics using various sampling and monitoring methods at different crop phenology stages is crucial for enhancing pest management and ecosystem service functioning. The present study assessed the influence of four trap types (Blue, Yellow, White, and Malaise) applied at four tomato developmental stages (start of planting, flowering, flowering fruit development and harvest) on insect diversity in northeastern Tunisian open-field conditions. A total of 1771 insect individuals belonging to seven orders and 31 families were trapped, with the order Hymenoptera being the most common in the sampled plots, which was represented by 25 families. Trap type exerted a strong effect on both abundance and alpha diversity parameters. Yellow pan traps showed the highest diversity, with family richness (S) ranging from 1 to 16, Shannon diversity (H) reaching 2.54, Simpson (Is) diversity ranging from 0.72 to 0.90 and Pielou’s evenness (J) ranging from 0.83 to 0.98. Blue and white traps displayed intermediate diversity (Blue: S = 6 and H = 1.7; White: S = 7 and H = 1.6), while Malaise traps captured the least diverse assemblages (S = 4, H = 1.2 and Is = 0.65). These differences were highly significant (p < 0.05). Phenological stage significantly structured Hymenoptera diversity. Richness peaked at the start of planting (S = 1–16 and H up to 2.54) and declined sharply at harvest (S = 1–6). Pollinator families (Apidae, Halictidae, Megachilidae) were the most abundant during flowering, whereas parasitoid families (Braconidae, Eulophidae) dominated during the fruit development stage. Beta diversity analyses (NMDS, stress = 0.25) and PERMANOVA showed that trap type and phenological stage jointly explained 15.5% of the variation in community composition (R² = 0.155, p = 0.014). Although a strong taxonomic overlap among traps was observed, Indicator Value analysis revealed significant trap-specific associations, including the family Andrenidae with Blue traps and the family Scoliidae with White and Yellow traps. Overall, the results of the present study demonstrate that both trap type and crop phenology significantly influence insect population diversity. A multi-trap sampling strategy combining colored pan traps and Malaise traps could be recommended to accurately characterize insect communities and associated ecosystem services in Mediterranean open-field tomato systems. Full article

Neonicotinoid insecticides were initially hailed as safer alternatives to organochlorine and organophosphate pesticides due to their perceived lower toxicity to non-target organisms. However, it has been recently discovered that sublethal exposure to neonicotinoids negatively affects beneficial arthropods that are essential for a functional ecosystem. These beneficial arthropods include pollinators, biological control agents, and decomposers. This review synthesizes current research on the physiological, behavioral, and reproductive consequences of neonicotinoids on non-target arthropods and their broader ecological impact. The chemical and physical properties of neonicotinoids raise concerns about long-term ecological consequences of neonicotinoid use because these chemicals are persistent in plants and soil, which contributes to prolonged exposure risks for organisms. Sublethal doses of neonicotinoids can disrupt the ecological services provided by these organisms by impairing essential biological processes including motor function, odor detection, development, and reproduction in insects, while also altering behavior such as foraging, mating, and nesting. Furthermore, neonicotinoid exposure can alter community structure, disrupting trophic interactions and food web stability. Recognizing the sublethal impacts of neonicotinoids is critical for the development of more sustainable pest management strategies. It is imperative that future research investigates the underlying mechanisms of sublethal toxicity and identifies safer, more effective approaches to neonicotinoid-based pest control to mitigate adverse ecological effects. Incorporating this knowledge into future environmental risk assessments will be essential for protecting biodiversity and maintaining ecosystem functionality. Full article

Land maxing in cultivated ecosystems can improve upon other agroecological approaches because in this approach social, economic and ecological benefits are maximized within the available land, in part through the careful selection of plant species with specific benefits, e.g., biodiversity conservation, provision of ecological services, diversifying and improving farmer incomes. In this approach, plant species selected for improving farmer incomes are those providing non-timber marketable products, and plant species selected for biodiversity conservation and provision of ecological services can be identified quantitatively via ecological network theory. Here, we demonstrate using ecological network theory to identify (a) farm management practices associated with ecological network indices, and (b) key plant species that farmers can plant to maximize the potential for their land to support bees and pollination services. In this study we quantified bee-plant interaction networks within 10 agroforests, and compared results between the entire bee community and the subsetted stingless bee community. Bee abundance increased with flowering plant richness, explaining 9% of the variance (R² = 0.09; β = 0.05, SE = 0.03). Diverse agroforests with higher numbers of tree species supported less connected (R² = 0.67; β = −0.08, SE = 0.02), less nested (R² = 0.53; β = −0.05, SE = 0.01), and more specialized (R² = 0.63; β = 0.07, SE = 0.02) and modular (R² = 0.37; β = 0.05, SE = 0.02) bee-plant networks. Some key plant species with the strongest impacts on network structure and stability were shared between the entire bee-plant and the stingless bee-plant networks. We recommend that farmers plant the species highlighted in this study to maximize the value of their diverse agroforests to support bee communities and pollination services. Full article

Urban densification threatens biodiversity, yet conventional greenspace expansion is constrained by limited land availability. Living wall systems (LWS) offer potential biodiversity enhancement through vertical green infrastructure, though their ecological value remains underexplored. This study evaluated the biodiversity performance of three LWS in Plymouth, UK, using multi-taxa surveys to assess invertebrate communities, bird assemblages, and bat activity. A scoping review of 2638 publications revealed limited research on LWS biodiversity, with only 27% of biodiversity-focused papers referencing specific species. Field surveys employed standardised protocols including flower-visiting pollinator observations, spider assessments, soil invertebrate extraction using Tullgren funnels, acoustic bird monitoring, and bat emergence surveys across soil-based and hydroponic systems. Results demonstrated that soil-based LWS supported significantly higher invertebrate diversity than hydroponic systems, with 481 soil invertebrates recorded across 19 families. Plant species composition strongly influenced biodiversity outcomes, with Hedera helix, Erigeron karvinskianus, and Lonicera japonica attracting the most pollinator species (5 each). Bird abundance was significantly higher at LWS sites compared to control areas, with confirmed breeding by three species. However, current UK Biodiversity Net Gain frameworks undervalue LWS contributions due to their classification as artificial habitats. These findings indicate that appropriately designed soil-based LWS can deliver meaningful urban biodiversity benefits when integrated with strategic plant selection and species-based valuation approaches. Full article

Habitat fragmentation poses a serious threat to bee communities, which are essential for pollination and biodiversity conservation. This study evaluated how habitat fragmentation in an oak forest in Zapopan, Mexico affected bee diversity, abundance, feeding niches, and bee–plant interaction networks. We compared a protected natural area with a nearby forest fragment that has been isolated from the main forest by urbanization for the past 10 years. Wild bee abundance and species richness in the fragmented area decreased by 74% and 70%, respectively, compared to the natural area, accompanied by a significant reduction in diversity. Community composition shifted mainly due to species loss; furthermore, there was persistence of generalist species such as Apis mellifera, which became more abundant in the forest fragment. Feeding niches in the fragmented area were narrower according to the Levin index, reflecting more restricted diets and increased interspecific competition. Interaction networks were simplified, showing fewer interactions, loss of specialist bees, and decreased equitability, although network specialization remained stable, and an almost-total turnover in interactions indicated a reconfiguration of pollination patterns. These findings suggest that fragmentation negatively affects bee community structures and their interactions with plants, potentially compromising pollination and ecosystem services. Conservation of protected areas and restoration of disturbed sites with native plants are recommended to support the recovery and stability of bee communities and their ecological interactions. Full article

Pollinating insects are the most important pollinators in nature; they pollinate vegetables, fruits, oil crops, and wild plants, so that crop yields can be increased, wild plants can live and reproduce, and human food security and ecosystem stability are maintained. To identify the pollination network of plants–insects and the pollination service value in Hanzhong City, the methods of random net capture and transect counting in field work were used. The agricultural statistical data from Hanzhong City in 2023 was combined in the analysis. The results showed that Hanzhong City is rich in pollinator resources, with a total of 80 species of pollinators and 59 species of pollinating crops and wild nectar plants. The abundant pollinator resources provide sufficient pollination services for the production of local vegetables, fruits, and oil crops. The characteristics of the pollination networks are obvious, showing the structural characteristics of low connectivity, medium nesting, and low network specialization. In 2023, the pollination service value of pollinators in Hanzhong City was CNY 3524–4878 billion, accounting for 10.02–13.87% of the city’s agricultural output value of the year. Suggestions for the protection of pollinators in Hanzhong City: Reduce the use of pesticide, support beekeeping, intercrop nectar plants, and rationally plant crops. Full article

This perspective paper examines the multifaceted cultural ecosystem services (CESs) provided by wild bees with particular attention to the spiritual, medicinal, and traditional knowledge-based dimensions associated with Afrotropical stingless bees. We integrate these insights within a biocultural framework, highlighting their relational values and arguing that the systematic omission of these cultural roles in pollinator research, policy and conservation constitutes a form of epistemic injustice. We also argue that the systematic recognition and documentation of these cultural roles are critical to advancing more inclusive and effective conservation frameworks. Based on a synthesis of field observations, photo-documentation, and literature review, we propose future research and policy directions aimed at embedding these cultural values more comprehensively within biocultural conservation strategies. Full article