Search Results (11,224)

The premature senescence of red raspberry leaves severely affects plant growth. In this study, the double-season red raspberry cultivar ‘Polka’ was used, with N₁₅₀ (0.10 g N·kg⁻¹) selected as the treatment group (T150) and N₀ (0 g N·kg⁻¹) set as the control (CK). This study systematically investigated the mechanism of premature senescence in red raspberry leaves under different nitrogen application levels by measuring physiological parameters and conducting a combined multi-omics analysis of transcriptomics and metabolomics. Results showed that T150 plants had 8.34 cm greater height and 1.45 cm greater ground diameter than CK. The chlorophyll, carotenoid, soluble protein, and sugar contents in all leaf parts of T150 were significantly higher than those in CK, whereas soluble starch contents were lower. Malondialdehyde (MDA) content and superoxide anion (O₂⁻) generation rate in the lower leaves of T150 were significantly lower than those in CK. Superoxide sismutase (SOD) and peroxidase (POD) activities in the middle and lower functional leaves of T150 were higher than in CK, while catalase (CAT) activity was lower. Transcriptomic analysis identified 4350 significantly differentially expressed genes, including 2062 upregulated and 2288 downregulated genes. Metabolomic analysis identified 135 differential metabolites, out of which 60 were upregulated and 75 were downregulated. Integrated transcriptomic and metabolomic analysis showed enrichment in the phenylpropanoid biosynthesis (ko00940) and flavonoid biosynthesis (ko00941) pathways, with the former acting as an upstream pathway of the latter. A premature senescence pathway was established, and two key metabolites were identified: chlorogenic acid content decreased, and naringenin chalcone content increased in early senescent leaves, suggesting their pivotal roles in the early senescence of red raspberry leaves. Modulating chlorogenic acid and naringenin chalcone levels could delay premature senescence. Optimizing fertilization strategies may thus reduce senescence risk and enhance the productivity, profitability, and sustainability of the red raspberry industry. Full article

In the present study, we evaluated the potential use of a humic substance (HS)-based biostimulant in mitigating drought stress in lettuce (Lactuca sativa L.) by comparing both root and foliar modes of application. To achieve this, lettuce plants were grown in a growth chamber on a solid substrate composed of vermiculite and perlite (3:1). Plants were exposed to drought conditions (50% of Field Capacity, FC) and 50% FC + HS applied as radicular (‘R’) and foliar (‘F’) at concentrations: R-HS 0.40 and 0.60 mL/L, respectively, and 7.50 and 10.00 mL/L, respectively, along with a control (100% FC). HSs were applied three times at 10-day intervals. Plant growth, nutrient concentration, lipid peroxidation, reactive oxygen species (ROS), and enzymatic and non-enzymatic antioxidants were estimated. Various photosynthetic and chlorophyll fluorescence parameters were also analyzed. The results showed that HS applications alleviated drought stress, increased plant growth, and reduced lipid peroxidation and ROS accumulation. HSs also improved the net photosynthetic rate, carboxylation efficiency, electron transport flux, and water use efficiency. Although foliar HSs showed a greater tendency to enhance shoot growth and photosynthetic capacity, the differences between the application methods were not significant. Hence, in this preliminary work, the HS-based product evaluated in this study demonstrated potential for alleviating drought stress in lettuce plants at the applied doses, regardless of the mode of application. This study highlights HS-based biostimulants as an effective and sustainable tool to improve crop resilience and support sustainable agriculture under climate change. However, further studies under controlled growth chamber conditions are needed to confirm these results before field trials. Full article

Oxybenzone (OBZ), an organic ultraviolet filter, is an emerging contaminant posing severe threats to ecosystem health. Using tobacco (Nicotiana tabacum) as a model plant, this study investigated the alleviation mechanisms of exogenous silicon (Na₂SiO₃, Si) and bamboo-based biochar (Bc) under OBZ stress. We systematically analyzed physiological and biochemical responses, including phenotypic parameters, reactive oxygen species metabolism, photosynthetic function, chlorophyll synthesis, and endogenous hormone levels. Results reveal that OBZ significantly inhibited tobacco growth and triggered a reactive oxygen species (ROS) burst. Additionally, OBZ disrupted antioxidant enzyme activities and hormonal balance. Exogenous Bc mitigated OBZ toxicity by adsorbing OBZ, directly scavenging ROS, and restoring the ascorbate-glutathione (AsA-GSH) cycle, thereby enhancing photosynthetic efficiency, while Si alleviated stress via cell wall silicification, preferential regulation of root development and hormonal signaling, and repair of chlorophyll biosynthesis precursor metabolism and PSII function. The mechanisms of the two stress mitigators were complementary, Bc primarily relied on physical adsorption and ROS scavenging, whereas Si emphasized metabolic regulation and structural reinforcement. These findings provide practical strategies for simultaneously mitigating organic UV filter pollution and enhancing plant resilience in contaminated soils. Full article

Background/Objectives: Senecio scandens Buch.-Ham. is a flavonoid-rich traditional medicinal plant with established immunomodulatory properties. However, the mechanisms underlying the immunoregulatory and intestinal protective effects of its flavonoid extract (Senecio scandens flavonoids—SSF) remain unclear. This study characterized SSF’s bioactive components and evaluated its efficacy against cyclophosphamide (CTX)-induced immunosuppression and intestinal injury. Methods: The constituents of SSF were identified using UHPLC/Q-Orbitrap/HRMS. Mice with CTX-induced immunosuppression were treated with SSF (80, 160, 320 mg/kg) for seven days. Immune parameters (organ indices, lymphocyte proliferation, cytokine, and immunoglobulin levels) and gut barrier integrity markers (ZO-1, Occludin, Claudin-1 protein expression; sIgA secretion; microbiota composition) were assessed. Network pharmacology combined with functional assays elucidated the underlying regulatory mechanisms. Results: Twenty flavonoids were identified in SSF, with six prototype compounds detectable in the blood. The SSF treatment significantly ameliorated CTX-induced weight loss and atrophy of the thymus and spleen. It enhanced splenic T- and B-lymphocyte proliferation by 43.6% and 29.7%, respectively; normalized the CD4⁺/CD8⁺ ratio (1.57-fold increase); and elevated levels of IL-2, IL-6, IL-10, TNF-α, IFN-γ, IgM, and IgG. Moreover, SSF reinforced the intestinal barrier by upregulating tight junction protein expression and sIgA levels while modulating the gut microbiota, enriching beneficial taxa (e.g., the Lachnospiraceae_NK4A136_group, Akkermansia) and suppressing pathogenic Alistipes. Mechanistically, SSF activated the TLR/MyD88/NF-κB pathway, with isoquercitrin identified as a pivotal bioactive constituent. Conclusions: SSF effectively mitigates CTX-induced immunosuppression and intestinal damage. These findings highlight SSF’s potential as a dual-functional natural agent for immunomodulation and intestinal protection. Subsequent research should validate isoquercitrin’s molecular targets and assess SSF’s clinical efficacy. Full article

Consumer interest in vegetarian, ethical, and clean-label foods is reviving the use of plant-derived milk coagulants. Cardosins from Cynara cardunculus (“thistle”) are aspartic proteases with strong clotting activity, yet their technological impact in cheese remains under-explored. This study compared a commercial thistle extract (PC) with traditional bovine rennet rich in chymosin (AC) during manufacture and 60-day ripening of Caciotta cheese. Classical compositional assays (ripening index, texture profile, color, solubility) were integrated with scanning electron microscopy, three-dimensional surface reconstruction, and descriptive sensory analysis. AC cheeses displayed slower but sustained proteolysis, yielding a higher and more linear ripening index, softer body, greater solubility, and brighter, more yellow appearance. Imaging revealed a continuous protein matrix with uniformly distributed, larger pores, consistent with a dairy-like sensory profile dominated by milky and umami notes. Conversely, PC cheeses underwent rapid early proteolysis that plateaued, producing firmer, chewier curds with lower solubility and darker color. Micrographs showed a fragmented matrix with smaller, heterogeneous pores; sensory evaluation highlighted vegetal, bitter, and astringent attributes. The data demonstrate that thistle coagulant can successfully replace animal rennet but generates cheeses with distinct structural and sensory fingerprints. The optimization of process parameters is therefore required when targeting specific product styles. Full article

Soil salinization severely limits plant growth and productivity. Mulberry (Morus alba L.), an economically and ecologically important tree, is widely cultivated, yet its salt-tolerance mechanisms at the seedling stage remain insufficiently understood. This study investigated the physiological and biochemical responses of two-year-old mulberry (‘Tailai Sang’) seedlings subjected to six NaCl treatments (0, 50, 100, 150, 200, and 300 mmol L⁻¹) for 28 days. Results showed that growth parameters and photosynthetic gas exchange exhibited dose-dependent declines. The reduction in net photosynthetic rate (Pn) was attributed to both stomatal limitations (decreased stomatal conductance) and non-stomatal limitations, as evidenced by a significant decrease in the maximum quantum efficiency of photosystem II (Fv/Fm) under high salinity. To cope with osmotic stress, seedlings accumulated compatible solutes, including soluble sugars, proteins, and proline. Critically, mulberry seedlings demonstrated effective ion homeostasis by sequestering Na⁺ in the roots to maintain a high K⁺/Na⁺ ratio in leaves, a mechanism that was compromised above 150 mmol L⁻¹. Concurrently, indicators of oxidative stress—malondialdehyde (MDA) and H₂O₂—rose significantly with salinity, inducing the activities of antioxidant enzymes (SOD, CAT, APX, and GR), which peaked at 150 mmol L⁻¹ before declining under extreme stress. A biomass-based LC₅₀ of 179 mmol L⁻¹ NaCl was determined. These findings elucidate that mulberry salt tolerance is a coordinated process involving three key mechanisms: osmotic adjustment, selective ion distribution, and a robust antioxidant defense system. This study establishes an indicative tolerance threshold under controlled conditions and provides a physiological basis for further field-based evaluations of ‘Tailai Sang’ mulberry for cultivation on saline soils. Full article

Background: Pain is a complex phenomenon characterized by unpleasant experiences with profound heterogeneity influenced by biological, psychological, and social factors. According to the National Health Interview Survey, 50.2 million U.S. adults (20.5%) experience pain on most days, with the annual cost of prescription medication for pain reaching approximately USD 17.8 billion. Theranostic pain nanomedicine therefore emerges as an attractive analgesic strategy with the potential for increased efficacy, reduced side-effects, and treatment personalization. Theranostic nanomedicine combines drug delivery and diagnostic features, allowing for real-time monitoring of analgesic efficacy in vivo using molecular imaging. However, clinical translation of these nanomedicines are challenging due to complex manufacturing methodologies, lack of standardized quality control, and potentially high costs. Quality by Design (QbD) can navigate these challenges and lead to the development of an optimal pain nanomedicine. Our lab previously reported a macrophage-targeted perfluorocarbon nanoemulsion (PFC NE) that demonstrated analgesic efficacy across multiple rodent pain models in both sexes. Here, we report PFC-free, biphasic nanoemulsions formulated with a biocompatible and non-immunogenic plant-based coconut oil loaded with a COX-2 inhibitor and a clinical-grade, indocyanine green (ICG) near-infrared fluorescent (NIRF) dye for parenteral theranostic analgesic nanomedicine. Methods: Critical process parameters and material attributes were identified through the FMECA (Failure, Modes, Effects, and Criticality Analysis) method and optimized using a 3 × 2 full-factorial design of experiments. We investigated the impact of the oil-to-surfactant ratio (w/w) with three different surfactant systems on the colloidal properties of NE. Small-scale (100 mL) batches were manufactured using sonication and microfluidization, and the final formulation was scaled up to 500 mL with microfluidization. The colloidal stability of NE was assessed using dynamic light scattering (DLS) and drug quantification was conducted through reverse-phase HPLC. An in vitro drug release study was conducted using the dialysis bag method, accompanied by HPLC quantification. The formulation was further evaluated for cell viability, cellular uptake, and COX-2 inhibition in the RAW 264.7 macrophage cell line. Results: Nanoemulsion droplet size increased with a higher oil-to-surfactant ratio (w/w) but was no significant impact by the type of surfactant system used. Thermal cycling and serum stability studies confirmed NE colloidal stability upon exposure to high and low temperatures and biological fluids. We also demonstrated the necessity of a solubilizer for long-term fluorescence stability of ICG. The nanoemulsion showed no cellular toxicity and effectively inhibited PGE2 in activated macrophages. Conclusions: To our knowledge, this is the first instance of a celecoxib-loaded theranostic platform developed using a plant-derived hydrocarbon oil, applying the QbD approach that demonstrated COX-2 inhibition. Full article

Sources of renewable energy have attracted considerable attention. Their expanded use will have a substantial impact on both the cost of energy production and climate change. Solar energy is one efficient and safe option; however, solar energy harvesting sites, irrespective of the location, can impact the ecosystem. This experimental study explores the energy available inside and outside of novel miniature energy harvesting cages by measuring light intensity and power generated. Varying light intensity outside the cage has been utilized to study the remaining energy inside the cage of a flexible design, where the heights of the harvesting panels are parameters. Cages are built from custom photovoltaic panels arranged in a staircase manner to provide access to growing plants. The balance between power generation and biological development is investigated. Two different structures are presented to explore the variation of illumination intensity inside the cages. The experimental results show a substantial reduction in energy inside the cages. The experimental results showed up to 24% reduction in illumination inside the cages in winter. The reduction is even larger in summer, up to 57%. The results from the models provide a framework to study the possible impact on a biological system residing inside the cages, paving the way for practical farming with sustainable energy harvesting. Full article

This study conducts an in-depth analysis of the dynamic characteristics of a single reheat steam turbine generator unit and its speed control system under variable operating conditions. A comprehensive simulation model was constructed to comprehensively evaluate the impact of the heat extraction system on the dynamic behavior of the unit, which integrates the speed control system, actuator, single reheat steam turbine body, and once-through boiler dynamic coupling. This model focuses on revealing the mechanism of the heat extraction regulation process on the core operating parameters of the unit and the system frequency regulation capability. Based on the actual parameters of a 300 MW heat unit in a power plant in Guangxi, the dynamic response of the established model under typical dynamic conditions such as extraction flow regulation, primary frequency regulation response, and load step disturbance was simulated and experimentally verified. The results show that the model can accurately characterize the dynamic characteristics of the heat unit under variable operating conditions, and the simulation results are in good agreement with the actual engineering, with errors within an acceptable range, effectively verifying the dynamic performance of the heat system module and the rationality of its control parameter design. This study provides a reliable theoretical basis and model support for the accurate simulation of the dynamic behavior of heat units in the power system and the design of optimization control strategies for system frequency regulation. Full article

The tolerance of the fall armyworm (Spodoptera frugiperda) to plant-derived secondary compounds gradually increases with instars. Therefore, even if plant-based additives are applied at early stages, such as the second or third instar, they may have a differential impact on the ecofriendly control of S. frugiperda. In this study, S. frugiperda larvae were exposed to vanillic acid or sinapic acid at the second and third instar, and physiological and growth parameters were measured. The results showed that the effects of vanillic acid treatment on S. frugiperda were similar at the different instars. They can significantly affect the larval carboxylesterase, glutathione S-transferase, and mixed-function oxidase activities. By reducing larval food intake, food conversion, and utilization efficiency while increasing the food consumption rate, it inhibits weight accumulation. This leads to a significant extension of the development of both the larval and pupal stages, and the adult longevity was reduced. Treatment with sinapic acid at the second instar extended the negative effects on the pupal duration of S. frugiperda when compared to treatment at the third instar, but did not affect adult longevity. Therefore, vanillic acid treatment at the second or third instar stage, can play an important role in the ecofriendly control process of S. frugiperda. The results of this study are of great significance for integrated pest management. Full article

The growth of urban and industrial areas is accompanied by an increase in vehicle traffic, resulting in rising concentrations of various air pollutants. This is a global issue that causes environmental damage and risks to human health. The dataset presented in this research contains records with measurements of the air pollutants ozone (O₃) and carbon monoxide (CO), as well as meteorological parameters such as temperature (T), relative humidity (RH), and barometric pressure (BP). This dataset was collected using a set of low-cost sensors over a four-month study period (March to June) in 2024. The monitoring of air pollutants and meteorological parameters was conducted in a city with high industrial activity, heavy traffic, and close proximity to a petrochemical refinery plant. The data were subjected to a series of statistical analyses for visualization using plots that allow for the identification of their behavior. Finally, the dataset can be utilized for air quality studies, public health research, and the development of prediction models based on mathematical approaches or artificial intelligence algorithms. Full article

Straw fiber curtain contains a plant fiber blanket woven from crop straw, which is mainly used to protect embankment slopes from rainwater erosion. To investigate the erosion control performance of slopes covered with straw fiber curtains of different structural configurations, physical model tests were conducted in a 95 cm × 65 cm × 50 cm (length × height × width) test box with a slope ratio of 1:1.5 under controlled artificial rainfall conditions (20 mm/h, 40 mm/h, and 60 mm/h). The study evaluated the runoff characteristics, sediment yield, and key hydrodynamic parameters of slopes under the coverage of different straw fiber curtain types. The results show that the A-type straw fiber curtain (woven with strips of straw fiber) has the best effect on water retention and sediment reduction, while the B-type straw fiber curtain (woven with thicker straw strips) with vertical straw fiber has a better effect regarding water retention and sediment reduction than the B-type transverse straw fiber curtain. The flow of rainwater on a slope covered with straw fiber curtain is mainly a laminar flow. Straw fiber curtain can promote the conversion of water flow from rapids to slow flow. The Darcy-Weisbach resistance coefficient of straw fiber curtain increases at different degrees with an increase in rainfall time. Full article

The commercial propagation of strawberries is increasingly constrained by the incidence of both established and emerging soilborne pathogens, particularly under soil cultivation systems. Micropropagation represents an effective strategy to ensure the production of virus-free, true-to-type mother plants suitable for high-efficiency propagation. In this study, micropropagated mother plants of four short-day cultivars (‘Francesca’, ‘Silvia’, ‘Lauretta’, and ‘Dina’) and one ever-bearing advanced selection (‘AN12,13,58’) were cultivated under a controlled soilless system. Quantitative parameters including number of runners per plant, runner length, and number of tips per runner and per plant were assessed to evaluate propagation performance. Micropropagated mother plants exhibited a significantly higher stoloniferous potential compared to in vivo-derived mother plants (frigo plants type A), with the latter producing approximately 50% fewer propagules. Rooted tips of ‘Dina’ were further assessed under different fertigation regimes. The NPK 20–20–20 nutrient solution enhanced photosynthetic activity and shoot and root biomass (length, diameter, and volume via WinRHIZO analysis). These results confirm the suitability of micropropagated mother plants grown in soilless conditions for efficient, high-quality clonal propagation and support the integration of such systems into certified nursery production schemes. Full article

To address the problems of traditional methods that rely on destructive sampling, the poor adaptability of fixed equipment, and the susceptibility of single-view angle measurements to occlusions, a non-destructive and portable device for three-dimensional phenotyping and biomass detection in lettuce was developed. Based on the Structure-from-Motion Multi-View Stereo (SFM-MVS) algorithms, a high-precision three-dimensional point cloud model was reconstructed from multi-view RGB image sequences, and 12 phenotypic parameters, such as plant height, crown width, were accurately extracted. Through regression analyses of plant height, crown width, and crown height, and the R² values were 0.98, 0.99, and 0.99, respectively, the RMSE values were 2.26 mm, 1.74 mm, and 1.69 mm, respectively. On this basis, four biomass prediction models were developed using Adaptive Boosting (AdaBoost), Support Vector Regression (SVR), Gradient Boosting Decision Tree (GBDT), and Random Forest Regression (RFR). The results indicated that the RFR model based on the projected convex hull area, point cloud convex hull surface area, and projected convex hull perimeter performed the best, with an R² of 0.90, an RMSE of 2.63 g, and an RMSEn of 9.53%, indicating that the RFR was able to accurately simulate lettuce biomass. This research achieves three-dimensional reconstruction and accurate biomass prediction of facility lettuce, and provides a portable and lightweight solution for facility crop growth detection. Full article

Greenhouses have positively impacted plant production by allowing the cultivation of different crops per year. However, the accumulation of agricultural plastics, potentially contaminated with agrochemicals, raises environmental concerns. This work evaluates the combined effect of Bordeaux mixture and low-density polyethylene (LDPE) microplastics (<5 mm) on the growth of lettuce (Lactuca sativa L.) and soil microbial communities. Different levels of Bordeaux mixture (0, 100 and 500 mg kg⁻¹), equivalent to Cu(II) concentrations (0, 17 and 83 mg kg⁻¹), LDPE microplastics (0, 1% and 5%) and their combination were selected. After 28 days of growth, biometric and photosynthetic parameters, Cu uptake, and soil microbial responses were evaluated. Plant germination and growth were not significantly affected by the combination of Cu and plastics. However, individual Cu treatments influenced root and shoot length and biomass. Chlorophyll and carotenoid concentrations increased with Cu addition, although the differences were not statistically significant. Phospholipid fatty acid (PLFA) analysis revealed a reduction in microbial biomass at the highest Cu dose, whereas LDPE alone showed limited effects and may reduce Cu bioavailability. These results suggest that even at the highest concentration added, Cu can act as a plant nutrient, while the combination of Cu–plastics showed varying effects on plant growth and soil microbial communities. Full article