Search Results (9,229)

Lignocellulosic biomass is one of the most abundant renewable carbon resources available, currently used predominantly for energy generation through direct combustion, yet still underutilized as a feedstock for higher-value biochemical conversion. Its structural complexity and intrinsic recalcitrance continue to challenge efficient biological processing. Overcoming these barriers requires an integrated understanding of plant cell-wall architecture, pretreatment chemistry, enzymatic mechanisms, and process engineering. This review provides a clear and conceptually grounded synthesis of these elements, illustrating how they converge to enable the development of second-generation (2G) lignocellulosic biorefineries. This review examines the hierarchical organization of cellulose, hemicelluloses, and lignin; the principles and performance of modern pretreatment technologies; the synergistic action of cellulolytic systems, including lytic polysaccharide monooxygenases (LPMOs) and non-hydrolytic proteins such as swollenins; advances in C5/C6 sugar fermentation; and emerging strategies for lignin upgrading. In addition to a comprehensive analysis of the literature, representative industrial and experimental case studies reported in the literature are discussed to illustrate practical process behavior and design considerations. By integrating mechanistic insight with industrially relevant examples, this review highlights the technical feasibility, current maturity, and remaining challenges of lignocellulosic biorefineries, underscoring their strategic role in enabling a competitive, low-carbon bioeconomy. Full article

The recovery of extracellular polymeric substances (EPS) from activated sludge (AS) represents a promising strategy to transform wastewater treatment plants (WWTPs) into resource recovery facilities within a circular economy framework. In this study, EPS was extracted from an AS process in a full-scale WWTP, highlighting its catalytic and bioflocculant properties, which represent an innovation in the valorization of this biopolymer. The EPS was subsequently characterized in terms of polysaccharides, proteins, and enzymatic activities (amylase and lipase). The bioflocculation performance of the EPS was evaluated using activated sludge mixed liquor. Results showed that EPS recovery yields using 50 °C and 80 °C were 196.3 ± 38.2 mg EPS/g sludge and 283.5 ± 85.4 mg EPS/g sludge, respectively. Enzymatic assays confirmed amylase activity ranging from 100 to 350 U/g sludge according to the extraction temperature. Lipolytic activity (20 U/g sludge) was comparable to values reported in the literature for EPS from biological sludge. The addition of EPS significantly improved the sludge settling velocity (from 0.86 to 4.48 m/h) and the sludge volume index (from 118.6 to 35.5). However, EPS application also increased the resistance to filtration by 50% and reduced cellular respiration by approximately 40%. Overall, the findings demonstrate that EPS from activated sludge acts as an effective bioflocculant with relevant catalytic properties, highlighting its potential as a high-value biotechnological product while also pointing to operational challenges that require further optimization. Full article

In this paper, the effects of Poland’s forest management evolution after 1945 on forest biodiversity at the genetic level were analysed. Forest biodiversity changes across the two politically and economically different eras (socialism, 1945–1989, and democracy, from 1990) are interpreted using three indirect indicators: forest regeneration and expansion, tree genetic resources, and threatened forest species. In the era of socialism, the total area of regeneration and reforestation gradually decreased, with these activities relying almost exclusively on cultivated reproductive material. After 1990, there was a relative stabilisation in the total area, with a noticeable increase in the use of natural processes to diversify the tree gene pool. Work on verifying and protecting the forest tree seed base, as well as on assessing the conservation status of an increasingly wide range of organisms, began in the era of socialism; however, it was intensified only in the era of democracy. In the latter case, the increase in the number of endangered species suggests a potentially negative trend. However, the actual assessment of the changes is not entirely clear due to subsequent changes in threat classification and increased knowledge of the occurrence of individual species. Dilemmas and problems related to the following issues require further discussion and resolution or implementation of further measures: the consequences of past choices regarding planted trees; the use of natural regeneration; the reduction in the forest tree gene pool as a result of artificial selection; incomplete knowledge about threats to the forest gene pool; the continued impact of threats and the possibilities for counteracting them; and securing funding for measures to protect biodiversity at the genetic level. Full article

Land desertification poses a major ecological challenge and threatens agricultural productivity. This study investigated the seed endophytic microbiomes of desert plants as a potential resource for mitigating salt stress in crops. Using high-throughput sequencing, we characterized the bacterial and fungal communities within seeds of 12 desert plant species. Dominant taxa included Firmicutes (particularly Bacillus), Bacteroidota, Proteobacteria, Ascomycota, and Basidiomycota. Culturable bacteria were subsequently isolated from Haloxylon ammodendron (C.A.Mey.) Bunge (HB) and Hedysarum scoparium Fisch. & C.A.Mey. (HSA) seeds. These isolates were screened for plant growth-promoting (PGP) traits and tolerance to salt (NaCl) and alkali (NaHCO₃). Selected strains, including the high indole-3-acetic acid (IAA)-producing Bacillus sp. HB-4, were used to inoculate wheat (Triticum aestivum L.) under 150 mM NaCl or 150 mM NaHCO₃ stress. Inoculation with strain HB-4 significantly improved wheat growth under stress. This improvement was associated with increased chlorophyll and proline content, enhanced activities of the antioxidant enzymes catalase and peroxidase, and reduced levels of malondialdehyde, a marker of oxidative damage. Our results demonstrate that desert plant seeds harbor taxonomically distinct and functionally resilient endophytes. The successful application of a desert-adapted Bacillus strain to alleviate salt stress in wheat highlights the potential of such microbiomes as a novel source of inoculants for sustainable agriculture in saline-affected regions. Full article

‘Korlaxiangli’ pear occupies a pivotal position in the pear industry of Xinjiang, with both its cultivation area and total output ranking first in the region. However, ‘Korlaxiangli’ is vulnerable to freezing injury and has suffered frequent frost damage in recent years. A cold-tolerant superior individual plant was identified via preliminary field surveys and designated as cold-tolerant superior line 15-25-15 (abbreviated as ‘HY15’). To facilitate the large-scale application of this germplasm, tissue culture technology was adopted for its rapid propagation. Using spring-collected young shoots and autumn-collected dormant buds as explants, the optimal proliferation medium was determined to be DKW supplemented with 1.0 mg/L of 6-BA and 0.5 mg/L of NAA, while the optimal rooting medium was identified as 1/2 MS containing 2.5 mg/L of NAA and 15 g/L of sucrose. Transcriptome analysis revealed eight candidate genes potentially associated with shoot proliferation, among which ARF3 and ARR12 might be associated with high proliferation efficiency. This study provides a theoretical foundation and practical technical support for the conservation, genetic improvement, and establishment of efficient propagation systems of elite pear germplasm resources. Full article

Green roofs in Southern Europe are interest-growing nature-based solutions, capable of improving urban sustainability by positively impacting the water cycle, biodiversity, pollution, and, in some cases, energy consumption and carbon sequestration. Native plants adapted to Mediterranean climates exhibit drought-resistant traits, making them highly suitable for the challenging microclimate of green roofs. This microclimate features intense solar radiation, strong winds, and higher temperatures, in comparison to ground level, leading to increased atmospheric evaporative demand, driven by the interplay of radiation, wind, temperature, and humidity. Consequently, native plants from ecosystems resembling this microclimate are likely better suited for green roofs than local ground-level species. The current review synthesizes current knowledge on the use of native plants in Southern European green roofs, focusing on water management challenges given the region’s climate and scarce water resources. Out of roughly 12,500 native plant species in the Mediterranean basin, only about 124 have been examined in the past 20 years for green roof applications, with just 16% appearing in multiple scientific studies, highlighting a significant knowledge gap. The data indicate that ca. 85% of these species are perennials, valued for their low maintenance needs, a key consideration for green roof sustainability. Some of the studied species retain adequate aesthetic value when cultivated on green roofs with limited water availability. These species are mainly associated with four habitat types—rocky, coastal, dry, or well-drained environments—with a few linked to humid or adaptable conditions. This study aims to document the selection of drought-adapted native plant species best suited for green roof implementation in Southern Europe, contributing to enhancing sustainable urban design in the region, considering water management best practices and water use efficiency. Full article

Wolffia globosa (W. globosa), an edible aquatic plant of the Lemnaceae family, has gained increasing attention as a potential alternative protein and functional food ingredient due to its rapid biomass production, favorable amino acid profile, and micronutrient content. This review critically evaluates the current evidence on the nutritional composition, protein quality, reported bioactive properties, safety considerations, and regulatory status of W. globosa, focusing on its suitability for food applications. Literature data indicate that W. globosa biomass can contain substantial protein levels on a dry-weight basis, with reported protein quality metrics approaching those of some conventional plant proteins under specific processing conditions. In addition, studies have explored the high antioxidant, antihypertensive, and metabolism-related bioactivities of W. globosa, primarily based on in vitro and animal studies. However, human clinical evidence remains limited, and reported functional effects should be interpreted with caution. Regulatory assessments, including novel food authorization in certain jurisdictions, support its use as a food ingredient under defined conditions but do not substantiate health claims. Overall, W. globosa represents a promising plant-based food resource; nevertheless, further standardized compositional analyses, bioavailability studies, and well-designed human trials are required to substantiate its functional and nutritional properties. Full article

The growing demand for sustainable and distributed energy solutions has driven increasing interest in triboelectric nanogenerators (TENGs) as platforms for energy harvesting and self-powered sensing. Biowaste-based triboelectric nanogenerators (BW-TENGs) represent an attractive strategy by coupling renewable energy generation with waste valorization under the principles of the circular bioeconomy. This review provides a comprehensive overview of BW-TENGs, encompassing fundamental triboelectric mechanisms, material categories, processing and surface-engineering strategies, device architectures, and performance evaluation metrics. A broad spectrum of biowaste resources—including agricultural residues, food and marine waste, medical plastics, pharmaceutical waste, and plant biomass—is critically assessed in terms of physicochemical properties, triboelectric behavior, biodegradability, biocompatibility, and scalability. Recent advances demonstrate that BW-TENGs can achieve electrical outputs comparable to conventional synthetic polymer TENGs while offering additional advantages such as environmental sustainability, mechanical compliance, and multifunctionality. Key application areas, including environmental monitoring, smart agriculture, wearable and implantable bioelectronics, IoT networks, and waste management systems, are highlighted. The review also discusses major challenges limiting large-scale deployment, such as material heterogeneity, environmental stability, durability, and lack of standardization, and outlines emerging solutions involving material engineering, hybrid energy-harvesting architectures, artificial intelligence-assisted optimization, and life cycle assessment frameworks. Full article

As sessile organisms, plants adapt to their environments through shoot branching, a key determinant of plant architecture. However, the regulatory mechanisms governing this trait in cucumber remain incompletely understood. Although certain WRKY transcription factors have been implicated in branching regulation in other species, whether they are involved in cucumber branching has not yet been reported. In this study, we identified a transcription factor, CsWRKY50, whose expression positively correlates with branching in cucumber. The transcript level of CsWRKY50 was markedly elevated in the multi-branching mutant nwd and in high-branching cucumber varieties. Notably, CsWRKY50 showed the highest expression in axillary meristems compared to other tissues. Heterologous overexpression of CsWRKY50 in Arabidopsis significantly increased branch number and altered the transcriptional levels of several branching-related genes. Yeast two-hybrid assays confirmed that CsWRKY50 interacts with CsRAX5 and a truncated form of CsMYB84. The additive branching phenotype observed in the CsWRKY50-overexpressing Atbrc1 mutant indicates that CsWRKY50 may function independently of BRC1. Notably, RAX genes were up-regulated under these conditions. This work provides novel genetic resources and a theoretical foundation for improving plant architecture in cucumber cultivation. Full article

Agriculture in semi-arid regions faces increasing challenges from temperature extremes and moisture stress, necessitating climate-smart and resource-efficient production systems. This study examined maize–mushroom intercropping as a climate-smart strategy for semi-arid regions. Field experiments conducted at Tamil Nadu Agricultural University evaluated four maize planting geometries, with and without mulch, in 2022. Results showed that close-maize spacing (45 × 25 cm) with mulch moderated temperature, increased humidity, and improved mushroom yield and biological efficiency. The treatment achieved a land equivalent ratio above one, indicating superior land use efficiency. Optimal microclimatic conditions (26–33 °C; 80–98% RH) enhanced paddy straw mushroom growth, demonstrating that simple field-level modifications can stabilize microclimate and promote resilient farming in semi-arid ecosystems. Full article

Urban markets are key nodes for the persistence and adaptation of traditional edible plant knowledge, linking rural production with urban consumption. This study was based on monthly market surveys conducted throughout 2025 in an urban market in Baise City, Guangxi, China. A total of 54 edible plant taxa were recorded, including both native and introduced species, with herbaceous plants predominating alongside climbers, trees, and grasses. Ethnobotanical data were obtained through semi-structured interviews with 40 local informants (20 men and 20 women, aged 25–65 years) selected using purposive sampling, focusing on individuals actively involved in purchasing and preparing edible plants. High Cultural Food Significance Index (CFSI) values highlighted culturally central taxa, including Allium ascalonicum L., × Brassarda juncea (L.) Su Liu & Z.H. Feng, and Houttuynia cordata Thunb., reflecting frequent use and culinary–medicinal integration. Fidelity Level (FL) analyses identified species with strong consensus for specific therapeutic applications, such as × B. juncea, Alpinia galanga (L.) Willd., and Nelumbo nucifera Gaertn., while Informant Consensus Factor (FIC) values indicated moderate to high agreement across gastrointestinal, respiratory, inflammatory, and other health categories. These results underscore the persistence of the “food as medicine” concept, showing that edible plants function simultaneously as nutritional and preventive healthcare resources. The overlap of culinary and medicinal roles demonstrates dynamic food–medicine integration, with urban markets acting as cultural hubs that maintain dietary diversity, household food security, and ethnobotanical knowledge. Future studies should incorporate ethnozoological resources and longitudinal monitoring to capture the full scope of urban food–medicine systems. Full article

Understanding intraspecific functional trait variability (ITV) is crucial for elucidating plant functional strategies under environmental change. This study investigates the functional responses of 129 Mediterranean olive (Olea europaea L.) cultivars conserved in the Worldwide Olive Germplasm Bank of Marrakech (WOGBM), focusing on three key leaf traits: specific leaf area (SLA), specific leaf water content (SLWC), and leaf area (LA). Substantial ITV was observed, with variability predominantly driven by cultivar differences and geographic origin. LA accounted for the highest within-cultivar variability (43.60%), followed by SLWC (31.67%) and SLA (17.92%). Geographic origin significantly influenced trait expression, with eastern Mediterranean cultivars exhibiting conservative resource-use strategies (high SLWC, low SLA, and LA), while western cultivars displayed acquisitive strategies (high SLA and LA, low SLWC). Principal component analysis further differentiated eastern and western cultivars, reflecting biogeographical and evolutionary influences. The relationship between LA and climatic variables suggests climate-driven selection, where cultivars from wetter regions develop larger leaves to optimize light capture and carbon assimilation, whereas those from drier environments exhibit smaller leaves to reduce water loss. These findings highlight a trade-off between resource acquisition and conservation, supporting the leaf economic spectrum at the intraspecific level. This study underscores the importance of ITV in olive diversification and adaptation, providing insights for breeding, conservation, and climate resilience. A trait-based approach proves valuable for exploring domestication processes and plant responses to environmental gradients. Full article

Many companies in the mining industry include decarbonization of production among their key strategic goals as part of their internal sustainability strategy. This need is driven by a number of factors: stricter regulation in the area of carbon footprint (introduction of carbon taxes, emissions quotas, reporting requirements); sustained growth in demand for electricity and rising market prices; economic feasibility—the need to optimize operating costs and improve energy efficiency. This study provides a comprehensive technical and economic justification for implementing a hybrid power supply system—combining a solar power plant (SPP) and a gas engine power plant (GPP)—at Solidcore Resources’ Varvarinsky hub in Kazakhstan. The methodology includes modeling the energy balance of the real asset (156.9 GWh of annual energy consumption), calculating the output of a 22.6 MW SPP based on local GHI/PR/η parameters, forming and determining the adaptability coefficient Kₐ (proportion of PV in total monthly electricity generation), conducting an economic assessment (NPV, payback period, sensitivity), and inventorying CO₂ emissions under Scope 1–2. The SPP provides approximately 41.3 GWh of electricity generation per year, with an average annual K_a = 0.263; the 40 MW installed capacity of the gas piston power plant covers the residual demand, forming a stable daily and seasonal balance. The project demonstrates a positive NPV (After Tax) = USD 23.65 million with an estimated payback period of 10 years, while the cost of energy in extraction and processing is reduced by almost three times, and the total reduction in CO₂ emissions will be 51%. Thus, hybridization of energy supply systems is a practical compromise between reliability and decarbonization. Determining the adaptability coefficient K_a allows the flexibility of the system to be taken into account, shows how effectively the new energy system uses renewable energy sources, and can be used to optimize the operation of the energy system to achieve the company’s internal sustainable development goals. Full article

With the advancement of the dual carbon goals and the rapid increase in the proportion of new energy installations, the power system faces multiple challenges including insufficient flexibility resources, intensified fluctuations in generation and load, and reduced operational safety. Integrated energy systems (IESs), serving as key platforms for integrating diverse energy sources and flexible resources, possess complex internal structures and limited individual regulation capabilities, making direct participation in grid dispatch and market interactions challenging. To achieve large-scale resource coordination and efficient utilization, this paper investigates external characteristic modeling and cluster aggregation optimization methods for IES, proposing a comprehensive technical framework spanning from individual external characteristic identification to cluster-level coordinated control. First, addressing the challenge of unified dispatch for heterogeneous resources within IES, this study proposes an external characteristic modeling method based on operational feasible region projection. It constructs models for the active power output boundary, marginal cost characteristics, and ramping rate of virtual power plants (VPPs), enabling quantitative representation of their overall regulation potential. Second, a cluster aggregation optimization model for integrated energy systems is established, incorporating regional autonomy. This model pursues multiple objectives: cost–benefit matching, maximizing renewable energy absorption rates, and minimizing peak external power purchases. The Gini coefficient and Shapley value method are introduced to ensure fairness and participation willingness among cluster members. Furthermore, an optimization mechanism incorporating key constraints such as cluster scale, grid interaction, and regulation complementarity is designed. The NSGA-II multi-objective genetic algorithm is employed to efficiently solve this high-dimensional nonlinear problem. Finally, simulation validation is conducted on a typical regional energy scenario based on the IEEE-57 node system. Results demonstrate that the proposed method achieves average daily cost savings of approximately 3955 CNY under the optimal aggregation scheme, reduces wind and solar curtailment rates to 5.38%, controls peak external power purchases within 2292 kW, and effectively incentivizes all entities to participate in coordinated regulation through a rational benefit distribution mechanism. Full article

It has been well established that biofilm formation plays a critical role in the pathogenesis of various plant pathogenic bacteria. However, research on this process in Paracidovorax citrulli, the causal agent of bacterial fruit blotch (BFB) in cucurbits, remains limited. Through screening of the infection pathways of P. citrulli in sweet melon leaves, observing biofilm formation morphology at bacterial colonization sites, and detecting the activities of pathogenicity-related enzymes, this study revealed that P. citrulli readily colonizes Hami melon vascular tissues following inoculation via petiole immersion, petiole dipping, or vine injection. Dense biofilms were observed within the vascular bundles of symptomatic leaf veins. Furthermore, P. citrulli was confirmed to secrete cellulase and pectinase, with enzymatic activities increasing progressively as disease severity intensified. These findings suggest that BFB development in Hami melon is likely associated with the synergistic action of P. citrulli, biofilm-mediated occlusion of xylem vessels and hydrolytic degradation of plant cell walls, which may contribute to initial water-soaked lesions and subsequent vein-associated necrosis in leaf tissues. This study provides a theoretical foundation for further elucidation of the pathogenic mechanisms of P. citrulli. Full article