Search Results (284)

Reproductive efficiency in pigs is regulated by hormonal pathways that control follicular development at Day 15 of the estrous cycle, during corpus luteum regression. Miniature pigs are extensively employed as human-relevant models in biomedical research, yet their reproductive characteristics during mid-luteal regression remain inadequately characterized, limiting assessments of their translational reliability. Differences in follicular morphology, hormonal signaling, and vascular development may underlie their lower fertility compared to conventional pigs. In this study, follicular development after corpus luteum formation was compared between conventional pigs and minipigs using histological staining, immunofluorescence, hormonal assays, and transcriptomic profiling. The expression of VEGF, mTOR, LH, FSH, PAPP-A, and apoptosis markers was evaluated across the granulosa and thecal regions. Differential gene expression was analyzed using microarray data followed by GO categorization. Minipigs exhibited smaller follicles, reduced vascularization, and lower VEGF and MMP activity compared to conventional pigs. Expression of LH and PAPP-A was higher in conventional pigs, while minipigs showed relatively elevated E2 and FSH levels. Transcriptomic data revealed greater upregulation of cell-survival- and angiogenesis-related genes in conventional pigs, including genes involved in IGF pathways. Apoptosis and poor extracellular matrix remodeling were more pronounced in minipigs. Minipigs demonstrated impaired follicular remodeling and weaker hormonal signaling after corpus luteum formation, which likely contributed to their reduced reproductive efficiency. Understanding these species differences can guide breeding strategies and fertility management in biomedical and agricultural settings. Full article

Artificial insemination (AI) is a key breeding technique in the swine industry; however, the lack of reliable biomarkers for semen quality limits its effectiveness. Seminal plasma (SP) contains extracellular vesicles (EVs) that present a promising, non-invasive biomarker for semen quality. This study explores the biochemical profiles of boar SP to assess semen quality through near-infrared spectroscopy (NIRS) and proteomics of SP-EVs. Fresh semen from mature Duroc boars was evaluated based on sperm motility, classifying samples as Passed (≥70%) or Failed (<70%). NIRS analysis identified distinct variations in water structures at specific wavelengths (C1, C5, C12 nm), achieving high accuracy (92.2%), sensitivity (94.2%), and specificity (90.3%) through PCA-LDA. Proteomic analysis of SP-EVs revealed 218 proteins in Passed and 238 in Failed samples. Nexin-1 and seminal plasma protein pB1 were upregulated in Passed samples, while LGALS3BP was downregulated. The functional analysis highlighted pathways associated with single fertilization, filament organization, and glutathione metabolism in Passed samples. Integrating NIRS with SP-EV proteomics provides a robust approach to non-invasive assessment of semen quality. These findings suggest that SP-EVs could serve as effective biosensors for rapid semen quality assessment, enabling better boar semen selection and enhancing AI practices in swine breeding. Full article

The expansion of the livestock industry has led to an increase in biogas slurry discharge, which contains high levels of nitrogen (N) and phosphorous (P). Struvite precipitation is an effective method for the recovery of N and P from biogas slurry, and the recovered N and P can be applied as a slow-release fertilizer in agricultural production. To form an industrial chain for struvite recovery and application in agriculture, we investigated the factors affecting struvite recovery from biogas slurry generated on a pig farm and evaluated its efficacy as a fertilizer. The N and P recovery efficiency was higher when magnesium chloride (MgCl₂) was used as a magnesium (Mg) source compared with magnesium oxide (MgO), and the optimal reaction conditions were pH 10, a reaction time of 20 min, a stirring rate of 200 rpm, and a Mg/P/N ratio of 1.2:1.0:1.0, which achieved N and P recovery rates of 81.83% and 99.67%, respectively. To further investigate the commercial utility of using struvite recovered from biogas slurry as a fertilizer, the growth and content of nutrients in two common vegetables in China were measured. The vegetable quality-related parameters of bock choy (Brassica chinensis) improved as the proportion of struvite in the fertilizer increased. Fresh weight, soluble sugar, and soluble protein increased by 194.47%, 46.13%, and 82.42%, respectively. The quality-related parameters of water celery (Oenanthe javanica (Blume) DC.) increased with an increasing proportion of struvite (27.90 mg·g⁻¹ soluble sugar and 42.20 mg·g⁻¹ soluble protein). The application of struvite precipitated from biogas slurry in plant cultivation shows great potential and lays a solid foundation for the resourceful recovery and utilization of biogas slurry. Full article

This study examined total ammoniacal nitrogen (TAN) rejection by two reverse osmosis (RO) and two nanofiltration (NF) membranes as a function of pH for three ammonium salts to optimize conditions for a hybrid membrane system that can produce high-purity TAN streams suitable for reuse. The results showed that TAN rejection was significantly influenced by membrane type, feed pH, and the ammonium salt used. This study represents the first attempt to simulate real manure wastewater conditions typically found in pig manure. TAN rejection for (NH₄)₂SO₄ and NH₄HCO₃ reached up to 95% at pH values below 7, with the SW30 membrane showing the highest performance (99.5%), attributed to effective size exclusion and electrostatic repulsion of SO₄²⁻ and HCO₃⁻ ions. In contrast, lower rejection was observed for NH₄Cl, particularly with the MPF-34 membrane, due to its higher molecular weight cut-off (MWCO), which diminishes both exclusion mechanisms. TAN rejection decreased markedly with increasing pH across the BW30, NF90, and MPF-34 membranes as the proportion of uncharged NH₃ increased. The lowest rejection rates (<15%) were recorded at pH 11.5 for both NF membranes. These results reveal a notable shift in separation behavior, where NH₃ permeation under alkaline conditions becomes dominant over the commonly reported NH₄⁺ retention at low pH. This novel insight offers a new perspective for optimizing membrane-based ammonia recovery in systems simulating realistic manure wastewater conditions. TAN recovery was evaluated using a hybrid membrane system, where NF membranes operated at high pH promoted NH₃ permeation, and the SW30 membrane at pH 6.5 enabled TAN rejection as (NH₄)₂SO₄. This hybrid system insight offers a new perspective for optimizing membrane-based ammonia recovery in systems simulating realistic manure wastewater conditions. Based on NH₃ permeation and membrane characteristics, the NF90 membrane was operated at pH 9.5, achieving a TAN recovery of 48.3%, with a TAN concentration of 11.7 g/L, corresponding to 0.9% nitrogen. In contrast, the MPF-34 membrane was operated at pH 11.5. The NF90–SW30 system also achieved a TAN recovery of 48.3%, yielding 11.7 g/L of TAN with a nitrogen content of 1.22%. These nitrogen concentrations indicate that both retentate streams are suitable for use as liquid fertilizers in the form of (NH₄)₂SO₄. A preliminary economic assessment estimated the chemical consumption cost at 0.586 EUR/kg and 0.729 EUR/kg of (NH₄)₂SO₄ produced for the NF90–SW30 and MPF-34–SW30 systems, respectively. Full article

Artificial insemination (AI) is essential in intensive pig production, which significantly depends on semen quality from boars selected for health, genetics, and fertility. While AI aims to improve productivity, larger litters often result in smaller and less resistant piglets. Beyond fertility and genetic traits, boars also influence offspring health. This study investigated the relationship between sperm parameters of highly fertile boars and both reproductive outcomes and piglet physiological indicators. Multivariate analysis revealed significant paternal effects on blood markers reflecting organ function, including those of the pancreas, liver, and kidneys, as well as on glucose homeostasis, lipid metabolism, oxidative stress, protein and carbohydrate metabolism, muscle contraction, and neural signaling. Notably, sperm velocity was correlated with mitochondrial function, which is crucial for sperm motility, capacitation, DNA integrity, and embryo development—factors likely linked to healthier, more resilient offspring. Boars transmitting superior sperm velocity, erythropoiesis efficiency, and oxygen transport capacities produced piglets with better glucose regulation, growth, and resistance to neonatal hypoglycemia. These findings underscore the broader impact of sperm quality on offspring vitality and suggest that advanced sperm analysis could improve boar selection and enable more effective, health-oriented breeding strategies. Full article

Waste from livestock farms contains various pathogens, including eggs and larvae of helminths—pathogens of parasitic diseases harmful to animals and humans. One of the methods for their effective processing to obtain biofertilizer and biofuel is anaerobic digestion, which requires further improvement to completely suppress the viability of pathogenic microorganisms in mesophilic conditions. To this end, the use of anaerobic digestion under sulfate reduction conditions to suppress pathogens using biogenic hydrogen sulfide is promising. Consequently, this study aims to study the effect of a sulfur-containing additive such as phosphogypsum on the disinfection of pig manure during anaerobic digestion. Egg mortality was already found to increase significantly compared to the control (80% and more), even at a minimum concentration of phosphogypsum (5%), on the fifth day of the experiment. At the same time, the maximum effect (100% mortality of Trichuris suis eggs) was recorded at a 10% concentration of phosphogypsum, starting from the 10th day of the study. Our experiment showed that changes in anaerobic digestion conditions using phosphogypsum could positively affect digestate disinfection. However, further research is needed to optimize the conditions of the process for an effective combination of disinfection with the production of environmentally safe organic fertilizers and high-quality biogas with a high level of methane. Full article

The content of bioavailable forms of phosphorus (P) and potassium (K) in soil is essential for the proper functioning of agroecosystems. This study aimed to determine the effects of pig slurry (PS) and NPK mineral fertilizers on soil phosphorus (P) and potassium (K) fractions, the relationship between these fractions and basic soil agrochemical properties, and crop yield. The research material was collected from a long-term experiment established in 1955 in Prague-Ruzyně, Czechia. The effect of two constant factors was analyzed: manure application (control, PS) and different doses of NPK fertilizers (N₀P₀K₀, N₁P₁K₁, N₃P₂K₂, and N₄P₂K₂). A significant effect of fertilization on basic soil properties was demonstrated, including total soil carbon and nitrogen. PS and NPK fertilization also significantly affected the content of water-soluble and moderate labile fractions of P and K. These fractions were positively correlated with plant-available P and K (Mehlich 3). The best fertilization option, which resulted in the greatest increase in yield, was the use of PS and mineral fertilizers at the N₃P₂K₂ level. Increasing the nitrogen dose to the level of N₄ resulted in a decrease in the content of bioavailable forms of P and K in topsoil despite the application of PS. Full article

To effectively contribute to climate change mitigation, agronomists are increasingly focused on minimizing the application of synthetic fertilizers and pesticides while ensuring that crop yield and quality are not compromised. Plant biomass and organic fertilizers are known to improve soil quality, boost plant growth, and suppress diseases. However, their overall effectiveness remains limited, hence the need for further research to enhance their agricultural performance. This study aims to explore the potential application of two natural sources (manure digestate and crop Artemisia dubia) for crop fertilization and protection. During the growing season, winter wheat was fertilized twice (21–25 BBCH and 30–35 BBCH) with synthetic, organic (pig manure digestate), and combined synthetic–organic fertilizers. Artemisia dubia biomass was incorporated before sowing and planted in strips. The soil chemical composition, crop overwintering, weediness, and diseases were assessed after two years of the respective treatments. The results showed that the organic carbon content increased by 1–5% after fertilizing winter wheat with pig manure digestate and combining fertilizers (organic and synthetic). Additionally, fertilizer or pesticide use had a significant effect on the soil pH process. Combining synthetic and organic fertilizers increased the amount of mobile phosphorus in the soil by 38%. In conclusion, combining synthetic fertilizers with organic fertilizers is the most effective approach to maintain healthy soil conditions and prevent damage to sprouts in the soil. Overall, our findings offer more opportunities for organic and sustainable agricultural processes by integrating pig manure digestate and Artemisia dubia biomass as a natural approach to minimizing synthetic fertilizer and pesticide use. Full article

Ammonia (NH₃) is a significant air pollutant with major environmental and health impacts, largely attributed to agriculture. Pig production is a major contributor, accounting for 25% of livestock NH₃ emissions. This study developed a new system based on gas-permeable membranes (GPM) technology for NH₃ recovery from the atmosphere obtaining a solution of ammonium sulfate as the resulting fertilizer product. Various experimental configurations were evaluated in the novel system using a synthetic NH₃-emitting solution. The optimal arrangement was a GPM system with recirculation of the generated NH₃ and without recirculation of the acidic trapping solution, yielding a nitrogen (N) recovery rate of up to 237 g m⁻² d⁻¹. Subsequent tests using pig manure (PM) at varying durations achieved rates of up to 73 g m⁻² d⁻¹, representing a four-fold increase in N capture efficiency compared to previous research. The influence of manure temperature on NH₃ emission and capture were analyzed, simulating the possible differences between seasons (summer and winter), and revealing higher N recovery rates at elevated temperatures. At 21.5 °C, the recovery rate was 7.7 g m⁻² d⁻¹, while increased temperatures of 38.8 °C and 49.3 °C yielded rates of 15.9 and 27.2 g m⁻² d⁻¹, respectively. Full article

The balanced application of organic and chemical fertilizers is essential for maintaining soil fertility and crop productivity. To optimize nitrogen (N) balance and maize yield through integrated pig slurry and straw mulching management, a split-plot field experiment was conducted in Northeast China. The study included two straw treatments (straw mulching, S; no straw, NS) and three substitution levels of pig slurry for chemical fertilizer (0%, 20%, and 40%; denoted as M0, M20, and M40). Parameters evaluated included N balance, maize biomass, soil available N, and the mineral N to TN ratio (mineral-N/TN), measured across 0–100 cm at key maize growth stages. Results showed that pig slurry substitution significantly increased soil DON, mineral N, and mineral-N/TN in the topsoil (0–20 cm) at the maize seeding stage and decreased mineral-N/TN at the maize milk (10–40 cm) and maturity (80–100 cm) stages. Meanwhile, straw mulching reduced NH₄⁺-N accumulation in the 0–10 cm of topsoil at the seeding stage, decreased NO₃⁻-N in the 0–40 cm soil layer from the jointing to maturity stages, and lowered the mineral-N/TN ratio in the topsoil, thereby mitigating the risk of N leaching. Notably, the combination of pig slurry substitution and straw mulching slightly increased DON and NO₃⁻-N in the topsoil while significantly reducing the mineral-N/TN in the deep soil layer at the seeding and milk stages. Pig slurry substitution significantly improved maize yield, N uptake, and N use efficiency (NUE). The highest maize yield (14,628 kg ha⁻¹) was observed in the S-M20 treatment, representing a 19% increase compared to NS-M0. N balance analysis indicated that pig slurry substitution alone increased maize yield and N uptake but depleted soil N, whereas straw mulching maintained N surplus. The findings highlight that combining pig slurry with straw mulching optimizes soil N availability and improves sustainable N management and crop productivity in agroecosystems. Full article

Sustainable fertilization using local resources such as manure is crucial for soil health. This study evaluated the potential of guinea pig manure to replace mineral fertilizers in hard yellow maize (hybrid INIA 619) under Peruvian coastal conditions. A split-plot design tested four doses of guinea pig manure (0, 2, 5, 10 t⋅ha⁻¹) and four levels of mineral fertilization (0%, 50%, 75%, 100%). The study assessed plant height, ear characteristics, yield, and nutritional quality parameters. The results indicated that 100% mineral fertilization led to the highest plant height (229.67 cm) and grain weight (141.8 g). Yields of 9.19 and 9.08 t⋅ha⁻¹ were achieved with 5 and 10 t⋅ha⁻¹ of manure, while 50% mineral fertilization gave 8.8 t⋅ha⁻¹, similar to the full dose (8.7 t⋅ha⁻¹). The protein content was highest with 10 t⋅ha⁻¹ of manure combined with mineral fertilization. However, no significant differences were found between the 50%, 75%, and 100% mineral fertilizer doses. In conclusion, applying guinea pig manure improved nutrient use efficiency, yield, and grain protein quality in maize, reducing the need for mineral fertilizers by up to 50%. This provides a sustainable fertilization strategy for agricultural systems. Full article

Anaerobically digested liquid pig manure (LPM) is enriched with nutrients and thus can be used as an alternative nutrient source and substitute for chemical fertilizer (CF) in rice (Oryza sativa L.) farming. However, there are concerns regarding the contamination of the surrounding water due to the discharge of ponding water containing dissolved organic carbon (DOC), nitrogen (N), and phosphorus (P) from rice paddies fertilized using LPM. This study investigated the effects of the co-application of fly ash (FA) and zeolite (Z) amendments (FAZ amendments) on the concentration of DOC, N, and P in the ponding water of rice paddies fertilized with LPM at two different rates (standard (LPM_S) and double (LPM_D) at 11 and 22 g N m⁻², respectively). Rice was cultivated using four nutrient treatments, including no input, CF (11 g N m⁻²), LPM_S, and LPM_D, with or without FAZ amendments. When FAZ was not amended, LPM_S and LPM_D application increased the DOC concentration by 32% and 41%, respectively, compared to CF treatments (11 g N m⁻²), reflecting a high DOC concentration in LPM. The total N and P concentrations in the ponding water were lower in LPM_S treatment (by 5 and 8%, respectively) but higher (by 94% and 47%, respectively) in LPM_D treatment compared to CF treatments in the absence of FAZ, indicating a high potential for water pollution with a double LPM application rate. With a given nutrient treatment, FAZ amendments decreased DOC by 15–39%, supporting the immobilization of DOC by Z. FAZ consistently decreased the NH₄⁺ concentration by 6–51% across the nutrient treatments, likely via the sorption of NH₄⁺ onto the negatively charged sites of Z, but its effect on total N concentration was not consistent. Unexpectedly, total P concentration increased (by 77–167%) following the FAZ amendment. FAZ amendments tended to increase rice biomass and grain yield for LPM treatments, but these rice growth parameters were poor compared to CF regardless of FAZ amendment. Our results show that the application of LPM as a complete replacement for CF may hamper rice yield while increasing the likelihood of water pollution with DOC and P, although the co-application of FAZ may help to reduce rice yield loss and decrease DOC and NH₄⁺ concentrations. Full article

Expression quantitative trait locus (eQTL) mapping is an effective tool for identifying genetic variations that regulate gene expression. An increasing number of studies suggested that SNPs associated with complex traits in farm animals are considered as expression quantitative trait loci. Identifying eQTLs associated with gene expression levels in the endometrium helps to unravel the regulatory mechanisms of genes related to reproductive functions in this tissue and provides molecular markers for the genetic improvement of high-fertility sow breeding. In this study, 218 RNA-seq data from pig endometrial tissue were used for eQTL analysis to identify genetic variants regulating gene expression. Additionally, weighted gene co-expression network analysis (WGCNA) was performed to identify hub genes involved in reproductive functions. The eQTL analysis identified 34,876 significant cis-eQTLs regulating the expression of 5632 genes (FDR ≤ 0.05), and 90 hub genes were identified by WGCNA analysis. By integrating eQTL and WGCNA results, 14 candidate genes and 16 fine-mapped cis-eQTLs were identified, including FRK, ARMC3, SLC35F3, TMEM72, FFAR4, SOWAHA, PSPH, FMO5, HPN, FUT2, RAP1GAP, C6orf52, SEL1L3, and CLGN, which were involved in the physiological processes of reproduction in sows through hormone regulation, cell adhesion, and amino acid and lipid metabolism. These eQTLs regulate the high expression of candidate genes in the endometrium, thereby affecting reproductive-related physiological functions. These findings enhance our understanding of the genetic basis of reproductive traits and provide valuable genetic markers for marker-assisted selection (MAS), which can be applied to improve sow fecundity and optimize breeding strategies for high reproductive performance. Full article

The aim of this study was to determine the effect of progressive pig slurry (PS) rates, applied over a 12-year period, on the molecular composition of soil organic matter in a calcareous soil. Annual organic matter rates of PS ranged from 1.0 to 4.8 Mg ha⁻¹. Humic acids (HAs) were extracted from field plots treated with PS, including a control (no PS applied). These HAs were analysed using pyrolysis–gas chromatography–mass spectrometry. The proportions of the 122 major compounds released from the soil HAs indicated that PS stimulated humification processes, with the degree of enhancement depending on the application rate. The applied PS contained a high proportion of aliphatic compounds, but only steroids and triterpenes accumulated in the HA soil fraction, and this was only observed at low PS rates. These results suggest that the application of PS leads to a dose-dependent increase in alkyl compounds, mainly alkanes and olefins. Aromatic compounds also showed a dose-dependent increase, but not in terms of the demethoxylated compounds typical of mature humic substances found in the original soil. Instead, the increase in aromatics was observed in the form of methoxyphenols, suggesting a recent incorporation of lignin derivatives from crop residues into the HA. Full article

Peanut yield and quality are often threatened by soil degradation under continuous cropping. Biochar has been known to improve the soil microbial community and plant resistance. However, studies on its functions to reduce soil degradation losses and improve the peanut yield are limited. A field peanut experiment was conducted in an Alfisol soil and biochar was applied at a rate of 20 t ha⁻¹ in 2022. The biochar was prepared from woodchip (WB) and maize straw (MB) feedstocks alone, as well as with co-composted biochar of the same feedstocks with pig manure labeled as WBSC and MBSC amendment, respectively. The conventional organic manure was applied as a control treatment (OM). All plots were base-fertilized with a mineral compound fertilizer of N-P₂O₅-K₂O (16-16-16, %) at 600 kg ha⁻¹. Topsoil (20 cm) and plant samples were collected at the time of peanut harvest. Soil quality, enzyme function, peanut growth traits, microbial abundance, and community composition were analyzed. Compared to OM, peanut yields increased by 22%, 23%, and 18% under WB, WBSC, and MBSC, respectively. The content of oleic acid increased by 4–5%, while the content of linoleic acid decreased by 7–9%, respectively, under biochar–compost treatments. However, biochar amendment alone showed non-significant changes in these fatty acids. The soil extracellular enzyme activity increased by 3.7–5.5% with biochar amendments and 6.4–10.1% with biochar–compost application. The enzyme activity ratio of hydrolase to non-hydrolase, of C cycling to N cycling, and of P cycling increased by 11.4–15.9%, 20.9–33.8%, and 14.7–23.5% under biochar amendments and by 20.5–25.0%, 17.4–39.0%, and 23.5–32.3% under biochar–compost, respectively. Overall, crop residue biochar enhanced peanut yield and quality by improving soil aggregation, enzyme functionality, and fungal community in line with the soil nutrient supply. Full article