Search Results (1,169)

Quantitative flowering phenotypes are needed to support breeding and harvest management in Hypericum perforatum L. (St. John’s wort), but manual flower assessment is slow and difficult to standardize under field conditions. UAV-Hyp is a multi-temporal UAV RGB dataset containing 12,653 high-resolution images acquired at 26 measurement dates across the complete flowering period of 15 H. perforatum accessions. The images represent variable illumination, soil moisture, weed pressure, and developmental stages. The dataset provides 59,163 plant bounding boxes and 107,054 flower bounding boxes. As an application example, cascaded YOLOv8 plant and flower detectors achieved mAP@0.50:0.95 values of 0.977 and 0.950, respectively. UAV-Hyp supports scalable flower quantification and the development of time-series phenotyping methods for genotype comparison and quality-oriented medicinal-plant breeding. Full article

This paper investigates the identity and circumstances of Nampo Joshi (南芳女史) (Ōzawa Nampo 大澤南芳, b. 1845; born Takei Tsuru), the woman artist who painted the kachō-ga (bird-and-flower painting) affixed to the reverse of James McNeill Whistler’s Blue and Silver: Old Battersea Bridge (1871/1873, The Hunterian, University of Glasgow). Completed in 1867, as the Edo period ended, Nampo’s work reached Europe with remarkable speed, raising the question of how a painting by a Japanese woman came into Whistler’s possession. Few historical documents directly pertaining to Nampo survive. However, she was the wife of the painter Ōzawa Nankoku (大澤南谷, 1844–?), and a systematic examination of records related to him—land registers, official gazettes, periodicals, exhibition catalogues, and diaries—reveals that the couple established themselves in Nihonbashi, the commercial centre of Tokyo. This broader archival investigation reconstructs who Nampo was: the socio-economic standing she and her husband achieved and the ways in which they extended their endeavours well beyond the traditional boundaries of their craft—participating energetically in the civic life of the new capital, in the exhibition culture of the Meiji period, and in educational and philanthropic activities. Crucially, the study demonstrates that masters deeply involved in Rangaku (Dutch Studies) networks advocating Japan’s opening to the world trained both artists and argues that this progressive intellectual milieu may provide essential context for understanding the swift acceptance of Nampo’s work in Europe. Full article

Red-flowered strawberries possess both floral and foliage ornamental characteristics, making them highly promising as landscape groundcovers and potted ornamental plants. To establish a standardized ornamental evaluation system and screen for superior germplasm, this study used 18 strawberry accessions from China and abroad as experimental materials. Twelve core ornamental indicators were selected across three dimensions: flowering period, morphological traits, and disease resistance. The Analytic Hierarchy Process (AHP) was employed to construct a multi-level ornamental value evaluation model. The results indicate the following weight rankings across the constraint layers: morphological traits (60.66%) > flowering period (27.71%) > disease resistance (11.63%). Among these, flower color (21.96%), plant flowering period (16.41%), corolla diameter (15.69%), and single-flower flowering duration (11.30%) were the core indicators determining ornamental value. The comprehensive score rankings showed that the accessions ‘140’, ‘Pink Panda’, ‘Summer Breeze-Cherry’, and ‘Summer Breeze-Rose’ exhibited the best overall ornamental performance. These accessions feature vibrant flower colors, a prolonged flowering period, abundant blooms, and good disease resistance. ‘Frisan’ and ‘246’ had the lowest composite scores, exhibiting significant shortcomings in ornamental traits. The AHP-based evaluation system established in this study provides a quantitative tool for ornamental trait assessment in red-flowered strawberries. The four elite accessions identifed using this system—‘140’, ‘Pink Panda’, ‘Summer Breeze-Cherry’, and ‘Summer Breeze-Rose’—can serve as priority candidates for protected landscape applications and container gardening in subtropical southern China. Full article

Background: Flower color is a well-defined trait in Platycodon grandiflorus, whereas little is known about the effects of flower color variation on the metabolic profiles of reproductive organs. Triterpenoid saponins and flavonoids have common precursors upstream, suggesting a potential carbon flux trade-off. Methods: Untargeted UPLC-MS/MS metabolomics was performed on the reproductive organs of purple- and white-flowered P. grandiflorus at six stages of flower development. Mfuzz time-series clustering, PCA, and metabolite correlation networks were used for data analysis. Results: Six clusters were assigned to 25 metabolites (17 triterpenoid saponins and 8 flavonoids). Flavonoid glycosides were found to possess a conserved inverted V-shaped accumulation pattern in both germplasms. By contrast, saponins derived from triterpenoids showed strong germplasm-dependent accumulation patterns. White-flowered plants showed sustained accumulation, with a peak at the young fruit stage. In purple-flowered plants, accumulation peaked transiently at the withering stage, followed by a decline. PCA validated that metabolic divergence increased with developmental progression. Conclusions: Based on the metabolomic profiles, we hypothesize a putative trade-off model in which floral color divergence may change upstream carbon flux allocation between the triterpenoid saponin and flavonoid pathways. The young fruit stage of white-flowered plants is a candidate harvesting period for bioactive saponins. These conclusions are based only on the pattern of metabolite accumulation and need to be validated by multi-omics. Full article

Pollination services provided by insects are fundamental to the productivity and quality of sweet cherry orchards. This study investigated the flower-visiting insect community and compared the pollination potential of A. mellifera and A. cerana in a sweet cherry orchard on the Loess Plateau of eastern Gansu, China. Field surveys were conducted during the blooming periods of 2025 and 2026. Visual observation and video recording documented flower-visiting insects across five daily time intervals. Foraging behavior, including flower-handling time and inter-visit interval, was measured in both bee species. Pollen loads on five body parts (head, thorax, abdomen, wings, and legs) were measured by hemocytometry. Standing nectar volume was measured by capillary extraction. Statistical analyses included generalized linear models, Friedman tests, and Pearson correlation analysis. Fourteen insect taxa were identified, among which Hymenoptera was the most dominant order. The main visitors were A. mellifera and A. cerana. The peak of A. cerana visitation was at 12:00–14:00, whereas A. mellifera showed a broader activity peak spanning 12:00–16:00. Apis mellifera exhibited a significantly longer single-flower handling time compared to A. cerana, and inter-visit intervals did not differ significantly between species. Pollen loads showed both species carried the loosest pollen on their legs, but A. cerana also maintained a substantial load on its thorax. Pollen loads did not differ significantly between the two species on any body region. Pollinator activity and standing nectar volume both peaked at 14:00–16:00, but bee visitation showed no linear correlation with environmental factors. Although A. mellifera was numerically dominant, our findings indicate that A. cerana may play a disproportionate role in pollination because of its unique pollen-carrying profile and earlier peak of activity. Therefore, maintaining both honeybee species alongside a diverse assemblage of flower-visiting insects is a prudent strategy for resilient orchard pollination. Full article

Abelmoschus manihot L. (Jinhuakui, JHK) is a Malvaceae plant with ornamental, medicinal, and edible value. It is rich in flavonoids, with a particularly high content of flavonols; however, current research on the molecular mechanisms underlying flavonol biosynthesis in JHK, particularly the upstream transcriptional regulatory networks, remains limited. In this study, JHK was used as the model plant to determine the patterns of flavonol accumulation in different tissues and during the flowering period. We cloned the AmFLS gene to validate its function and screened for and identified its upstream transcriptional regulators and interacting proteins. The results indicate that flavonols in JHK are primarily concentrated in floral organs, with the highest level observed during the budding stage. Furthermore, we found that AmFLS positively regulates flavonol biosynthesis and serves as the central rate-limiting gene. Additionally, AmMYB111 is a nuclear-localized R2R3-MYB repressor that directly binds to the AmFLS promoter and inhibits its transcription, thereby negatively regulating flavonol accumulation. Finally, the DELLA family proteins AmRGL2 and AmMYB111 can specifically interact with each other and actively promote the accumulation of flavonols. This study provides a preliminary elucidation of the molecular mechanism by which upstream transcriptional regulation of the AmFLS gene controls flavonol biosynthesis, offering a theoretical basis for further elucidating the regulatory network of flavonol metabolism in JHK and accelerating its industrial development. Full article

Walnut (Juglans regia L.) is a monoecious species with far more male than female flowers, yet the molecular mechanisms regulating sex differentiation remain largely unexplored. In this study, we investigated the effects of exogenous brassinolide (BR) on the female-to-male flower bud ratio and its regulatory mechanisms. We identified 23 April to 13 May as the critical physiological differentiation period for the ‘Xin xin 2’ variety. Exogenous application of 0.05 mg/L BR during this period significantly increased the female-to-male flower bud ratio across three consecutive years (2023–2025). Hormone metabolomics revealed that BR treatment induced sex-specific changes in ethylene and auxin contents. Transcriptomic analysis showed that BR differentially regulated MADS-MIKC genes in female and male buds. Integrated miRNA-mRNA analysis identified sex-specific regulatory modules targeting MADS-MIKC genes, including four in female buds and two in male buds. RT-qPCR and dual-luciferase assays confirmed that jre-miR156g directly targets the 3’-untranslated region of JrSPL5, with reciprocal expression patterns. Collectively, our findings demonstrate that exogenous BR increases the female-to-male flower bud ratio in walnut through multi-layered regulation involving hormone signaling, sex-specific expression of MADS-MIKC genes, and miRNA-mRNA regulatory cascades. This study provides a theoretical basis for breeding high-yielding walnut varieties by manipulating sex expression. Full article

Jujube flower disease (JFD) is a recurrent disorder affecting honeybees during the flowering period of Ziziphus jujuba, but its molecular basis remains unclear. To characterize JFD-associated molecular changes, we integrated widely targeted metabolomics and RNA sequencing (RNA-seq) in exposed asymptomatic jujube flower foragers (HC) and in bees showing characteristic JFD symptoms. Filtered metabolomic profiles showed clear separation between HC and JFD samples and revealed two major metabolite-level alterations. Tryptophan metabolism was the strongest enriched pathway among differentially abundant metabolites (Rich Factor = 14/16; FDR = $9.15\times {10}^{-7}$), whereas metabolites decreased in JFD were enriched mainly in galactose metabolism (Rich Factor = 7/16; FDR = $1.23\times {10}^{-4}$), amino sugar and nucleotide sugar metabolism (Rich Factor = 5/15; FDR = 0.00762), biosynthesis of nucleotide sugars (Rich Factor = 4/15; FDR = 0.0318), and starch and sucrose metabolism (Rich Factor = 3/8; FDR = 0.0318). RNA-seq detected 10,259 genes and identified 23 differentially expressed genes under the selected threshold, including 20 increased and 3 decreased genes in JFD bees. Although individual-gene differential expression was limited, preranked gene-set enrichment analysis identified moderate negative enrichment of the sugar supply/storage gene set (NES = −1.96; FDR = 0.024). The tryptophan/aromatic amino acid gene set was not enriched at the transcriptomic level, indicating that the tryptophan-related alteration was mainly observed at the metabolite level. Within these gene sets, HK showed the largest downward RNA-seq trend among the five displayed sugar-axis enzyme genes and a directionally concordant qRT-PCR trend; on this basis, HK was retained only as a candidate for future functional validation. Overall, these findings suggest that JFD is associated with altered tryptophan-related metabolism and reduced carbohydrate/nucleotide sugar-related metabolite abundance, with partial transcriptomic support for altered sugar supply and storage. This molecular profile provides candidate pathways and genes for future mechanistic investigation rather than established biomarkers, regulatory mechanisms, or evidence of disease causation. Full article

Asthma and seasonal allergic rhinitis are common conditions affecting health, wellbeing, and work place productivity. Current pollen monitoring requires experienced technicians and has a lag of at least 24 h, which limits usefulness for patient management. We evaluated agreement of daily pollen concentrations from an automated real-time sampler, the Swisens Poleno Jupiter, with a traditional Hirst-type trap using technician counting over a single monitoring season in England. We used regression models to assess the impact of weather covariates on differences. Total pollen showed a strong correlation (r = 0.74) but moderate Intraclass Correlation (ICC = 0.52) between devices, due to concentrations (total and taxa level) being higher for the manual than the automated sampler. For individual pollen taxa, strong correlation and good agreement was observed for Pinus, Alnus and Fraxinus throughout the monitoring period. Strong correlation but low/moderate agreement was observed for Poaceae (grass), Betula, Quercus and Taxus/Cupressus. No correlation or agreement was observed for Corylus and Platanus. Temperature explained most of the variability between samplers. We conclude that daily total pollen can be tracked using an automated Poleno, as can grass within its flowering season, but Poleno recognition algorithms developed for mainland Europe need further refinements for UK species. Improving agreement in quantification is of particular importance given that current allergenic thresholds are based on manual readings. Full article

Background: Ontogenetic flower color change is a prevalent adaptive phenomenon in plants, yet the temporal orchestration of multiple pigment pathways during rapid developmental transitions remains poorly understood. This study aims to elucidate the metabolic and transcriptional basis of the petal basal marking color transition in Weigela japonica from yellow through yellow-orange to purple-red within a 4-day blooming period. Methods: Metabolomic analysis of flavonoids, anthocyanins, and carotenoids was conducted using UPLC-MS/MS across four developmental stages (S0–S3). Differentially accumulated metabolites were screened based on VIP > 1, |Log₂FC| ≥ 1, and p < 0.05. Transcriptome sequencing was performed using the NovaSeq 6000 platform, and differentially expressed genes were identified using DESeq2. Weighted gene co-expression network analysis (WGCNA) was employed to identify hub genes associated with pigment accumulation. Key structural genes were validated by qRT-PCR. Results: Metabolomics revealed distinct stage-specific pigment accumulation: carotenoids peaked during the yellow-orange stage (S2), while anthocyanins and flavonoids surged in the final purple-red stage (S3). KEGG enrichment indicated that carotenoid metabolism operates as a discrete module, anthocyanin biosynthesis as a terminal-specific route, and flavonoid metabolism as an intermediary hub bridging the two pathways. Transcriptome analysis identified stage-specific gene expression patterns: PAL and C4H were active at S0–S1, CHS/CHI/F3H peaked at S1-S2, and DFR/ANS were maximally expressed at S2–S3. Carotenoid genes (PSY, LCY, ZDS) showed synchronous expression during the yellow-orange phase. WGCNA identified MYB44, bHLH92, and ERF3 as hub genes strongly correlated with the S3 anthocyanin surge. Conclusions: These findings suggest a sequential activation model in W. japonica, in which temporally partitioned pigment metabolism and stepwise transcriptional regulation converge to orchestrate rapid ontogenetic color change. The study provides a framework for understanding developmental color transitions in ornamental plants, though causal regulatory relationships remain to be functionally validated. Full article

The effective pollination period (EPP) is a key factor determining fruit set in sweet cherry, as it depends on the overlap between pollen tube growth and ovule longevity. This study investigated the duration and components of the EPP in three autochthonous cultivars (‘Stonska’, ‘Gomilička’, and ‘Tugarka’) grown in the Mediterranean region of Croatia over two consecutive flowering seasons. The EPP was determined under field conditions using sequential hand pollinations, while stigma receptivity, pollen tube growth, and ovule viability were assessed by fluorescence microscopy. Significant genotypic and seasonal variation in EPP and fruit set was observed. ‘Stonska’ and ‘Gomilička’ exhibited extended EPPs (9 and 11 days) and moderate to high fruit set. In contrast, ‘Tugarka’ showed a considerably shorter EPP (4–5 days) and consistently low fruit set (<10%). Stigma receptivity and pollen tube growth were not limiting factors for fertilization. However, rapid ovule degeneration in ‘Tugarka’, particularly under elevated temperatures during flowering, reduced the overlap between pollen tube arrival and ovule viability. These results indicated that ovule longevity plays a major role in determining fruit set and EPP under Mediterranean conditions. The study highlights genotypic differences in temperature sensitivity and provides insight into the potential impact of rising spring temperatures on sweet cherry production. Full article

Blue Vanda hybrids, including Vanda ‘Pakchong Blue’, frequently fail to flower during Thailand’s hot season (March–May), resulting in reduced market supply despite strong commercial demand. This study investigated flowering behavior and the effects of temperature management on growth, flowering, and flower quality of Vanda ‘Pakchong Blue’. In the first experiment, flowering behavior was examined through weekly histological observations of plants maintained under high-temperature conditions following inflorescence removal. Floral buds were initiated under high temperatures but failed to complete development and subsequently aborted. The period from floral initiation to anthesis was approximately 75 days. In the second experiment, plants were subjected to low-temperature treatments (18 ± 2 °C) for 0, 4, or 6 weeks and subsequently transferred to either an evaporative-cooling greenhouse (ECG) or a shaded greenhouse (SG). Plants grown in the ECG flowered during the off-season (March–May), whereas those grown in the SG flowered during the natural flowering season (August–September). Flowering in the ECG occurred 136–156 days earlier than in the SG and produced superior flower quality, including longer inflorescences and a greater number of florets. Exposure to 18 °C for 4 and 6 weeks delayed flowering by 24 and 45 days, respectively, but had no significant effect on vegetative growth. These findings demonstrate that successful off-season flowering and improved flower quality were primarily associated with cultivation in the evaporative-cooling greenhouse, whereas prolonged exposure to 18 °C delayed flowering without affecting vegetative growth. This study provides the first detailed characterization of floral bud development and flowering responses to different temperature-management strategies in this cultivar and highlights the potential of evaporative-cooling greenhouse technology for sustainable orchid production, climate-resilient floriculture systems, and year-round supply of high-quality flowers under tropical conditions. Full article

Green undersown crops (GUCs) are increasingly promoted as a sustainable management strategy to improve soil quality and ecosystem services in Mediterranean orchards, particularly in the calcareous, low-organic-matter soils typical of Eastern Spain. In this context, soil quality is understood as the soil’s overall functional capacity, integrating physical structure, chemical balance, and biological activity, whereas soil fertility refers specifically to the soil’s ability to supply nutrients to plants; soil quality therefore encompasses a broader set of ecosystem functions beyond nutrient provision. However, their effects on the calcareous, low-organic-matter soils typical of Eastern Spain remain insufficiently quantified. This study evaluates the effects of seeded undersown (grasses, legumes, and flower mixtures), spontaneous vegetation, and herbicide-managed bare soil on topsoil (0–15 cm) physicochemical and biological indicators in 54 plots across three irrigated persimmon orchard sites (Granja, Cargol, and Alginet) over 18 months of treatment in the València region (Eastern Spain). Seasonal sampling was conducted at the START (early winter) and END (late spring) of the experiment period. Soil measurements at both sampling times included soil organic matter (SOM), nitrogen (N), C/N ratio, pH, electrical conductivity (EC), soil respiration rate (RR), collembolan abundance, mite abundance, and the QBS-ar index of soil arthropods. Legumes increased SOM by +1.12%, grasses by +0.22%, whereas flower mixtures (−0.44%) and spontaneous vegetation (−1.36%) showed SOM reductions associated with rapid biomass turnover. RR increased under all GUCs (+0.06 to +0.16 g CO₂ m⁻² h⁻¹), and QBS-ar improved markedly under grasses (+26.6) and spontaneous vegetation (+36.7). EC decreased across all treatments (−16 to −84 µS cm⁻¹). These results were analysed using principal component analysis (PCA). Four PCA components explained 74% of the total variance, revealing functional gradients driven by SOM, N, EC, RR and mesofauna. After 18 months, microbial biomass carbon (MBC) increased by +45–60% under legumes, water-soluble organic carbon (WSOC) by +30–50% under legumes and flower mixtures, and the enzyme activities (EA) by +20–40% under all GUCs. Herbicide-managed soils showed reduced biological activity and detectable residues of glyphosate and oxyfluorfen. PCA and linear discriminant analysis (LDA) were used to identify functional gradients and treatment separation. GUCs significantly increased SOM, MBC, EA, and mesofauna abundance compared with herbicide treatments, which showed reduced biological activity and detectable residues of glyphosate and oxyfluorfen. Legumes and flower mixtures produced the strongest improvements in biological functioning due to higher MBC, WSOC, EA and RR. PCA and LDA confirmed clear separation between GUCs and herbicide-managed soils based on multivariate differences in SOM, N, EC, RR, MBC, WSOC and mesofauna indicators. Overall, GUCs modulated soil chemistry and biodiversity and enhanced soil functioning and biological quality, supporting their adoption as a sustainable management strategy in Mediterranean orchards. Legume-based covers are recommended for rapid biological activation, whereas grass-based covers favour longer-term SOM stabilisation. These findings highlight their role as key tools for improving soil resilience in Mediterranean persimmon orchards. Full article

Rhododendron tomentosum Harmaja is an evergreen Ericaceae species recognized as a rich source of biologically active phenolic compounds. However, information on seasonal variation in its non-volatile phenolics throughout the annual phenological cycle remains limited. Therefore, this study investigated the influence of phenological development on the phenolic composition of R. tomentosum leaves collected from a natural population in Lithuania between October 2023 and October 2024. Phenolic profiles were determined using a validated HPLC–PDA method. A total of 25 phenolic compounds were identified, comprising phenolic acids, flavan-3-ols, procyanidins, flavonoids, and coumarin derivatives. The highest total phenolic content was observed during autumn–winter dormancy, whereas the lowest levels occurred during fruit development. Procyanidins and phenolic acids predominated during dormancy, while flavonoids, particularly quercitrin, hyperoside, and isoquercitrin, accumulated during flowering. PCA revealed clear phenological grouping of samples and demonstrated distinct associations between dormancy and procyanidin-rich profiles and flowering and flavonoid accumulation. These findings highlight phenological stage as a key factor influencing phenolic composition and indicate optimal harvesting periods depending on the target phenolic compounds. Full article

The Turki Mountain Tomb, one of the three most representative Liao Dynasty tombs, has yielded numerous exquisite gold and silver artifacts during excavation that have drawn global attention for their superb craftsmanship and distinctive ethnic and period characteristics. However, their manufacturing technology had seldom been studied. In this paper, alloy composition analysis and surface microscopic observation were performed on the single-eared octagonal gold cup unearthed from the Turki Mountain Tomb, utilizing portable X-ray fluorescence spectroscopy (p-XRF) and an ultra-depth field microscope. The composition results at different base material locations of the gold cup were similar, with gold content ranging from 84% to 88% and silver content ranging from 10% to 13%. The p-XRF spectra at the exact center of the ring foot, as well as the pearl roundel on the abdominal ridge and rim, showed dominant Au with minor Ag content. Therefore, it could be concluded that the material of the gold cup was made of Au-Ag alloy. Microscopic observation preliminarily revealed that the manufacturing process involved casting, engraving, and welding. The single-eared octagonal gold cup exhibited numerous conspicuous shrinkage cavities, and it was inferred that the gold cup was formed using casting technology. After the cup body, ring foot, and finger pad were cast separately, they were welded together to form the complete gold cup. In addition, green solder and insufficient fusion of welding material were found between the weld seam of the cup body and the ring foot. The exterior surface of the gold cup was adorned with patterns, such as fish-toe circle, upward lotus motif, and pearl roundels. The average diameter of the fish-toe circle was 303 μm. By examining the overlapping conditions of engraving, it could be inferred that the proposed engraving sequence was to engrave the fish-toe circle first, followed by the flower patterns. As a representative of the exquisite artifacts from the Turki Mountain Tomb, the research of the composition and manufacturing technology of the gold cup provides reference data for the scientific analysis of Liao Dynasty gold and silver artifacts. Full article