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Keywords = organophosphate

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40 pages, 1142 KiB  
Review
The Blurred Lines Between New Psychoactive Substances and Potential Chemical Weapons
by Loreto N. Valenzuela-Tapia, Cristóbal A. Quintul, Nataly D. Rubio-Concha, Luis Toledo-Ríos, Catalina Salas-Kuscevic, Andrea V. Leisewitz, Pamela Cámpora-Oñate and Javier Campanini-Salinas
Toxics 2025, 13(8), 659; https://doi.org/10.3390/toxics13080659 - 1 Aug 2025
Viewed by 179
Abstract
The historical use of toxic chemicals to cause intentional harm has evolved from blister agents in World War I to highly lethal organophosphates and emerging families of chemicals, such as Novichok. In turn, medical or recreational substances like fentanyl, lysergamides, and phencyclidine pose [...] Read more.
The historical use of toxic chemicals to cause intentional harm has evolved from blister agents in World War I to highly lethal organophosphates and emerging families of chemicals, such as Novichok. In turn, medical or recreational substances like fentanyl, lysergamides, and phencyclidine pose a growing risk of hostile use, particularly related to the rapid proliferation of new psychoactive substances (NPSs). A narrative literature review was conducted covering specialized databases (PubMed, ScienceDirect, SciELO, Google Scholar) and sources from international organizations (OPCW, UNODC, ONU), analyzing historical and recent cases of the use of nerve agents in conflicts and the use of NPSs for hostile purposes. The main families of conventional agents (G, V, A series, and Novichok) and NPSs (lysergamides, PCP, fentanyl derivatives) were identified, highlighting their ease of synthesis, high toxicity profiles, and the regulatory gaps that facilitate their illicit production. In this scenario, it is essential to strengthen regulatory frameworks, surveillance systems, and ethical protocols in chemical research, as well as to promote international cooperation to prevent these substances from becoming chemical threats. Full article
(This article belongs to the Section Drugs Toxicity)
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21 pages, 3429 KiB  
Article
Embryonic Exposure to TPhP Elicits Osteotoxicity via Metabolic Disruption in Oryzias latipes
by Melissa C. Gronske, Jamie K. Cochran, Jessika D. Foland, Dereje Jima, David B. Buchwalter, Heather M. Stapleton and Seth W. Kullman
Toxics 2025, 13(8), 654; https://doi.org/10.3390/toxics13080654 - 31 Jul 2025
Viewed by 122
Abstract
Triphenyl phosphate (TPhP) is a widely used organophosphate flame retardant and plasticizer, raising concerns over its health impacts. This study examined the effects of embryonic TPhP exposure on axial skeletal development and metabolism in medaka (Oryzias latipes), a vertebrate fish model [...] Read more.
Triphenyl phosphate (TPhP) is a widely used organophosphate flame retardant and plasticizer, raising concerns over its health impacts. This study examined the effects of embryonic TPhP exposure on axial skeletal development and metabolism in medaka (Oryzias latipes), a vertebrate fish model relevant to human bone biology. Medaka embryos were exposed to 1 µM TPhP and assessed through early larval stages. TPhP impaired vertebral ossification, causing shortened centra and reduced cartilage in the caudal complex, alongside disrupted distribution of osteoblast-lineage cells. Key osteogenic genes were significantly downregulated at 14 days post fertilization, and transcriptomic analysis revealed altered mitochondrial pathways linked to skeletal disorders. Functionally, TPhP-exposed larvae showed reduced caudal fin regeneration and decreased metabolic rate and oxygen consumption, consistent with mitochondrial dysfunction. These findings indicate that TPhP disrupts bone development and metabolism by affecting osteoblast differentiation and mitochondrial regulation, highlighting the value of small fish models for studying environmental toxicants and bone metabolic disease risk. Full article
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13 pages, 2414 KiB  
Article
In Silico Characterization of Molecular Interactions of Aviation-Derived Pollutants with Human Proteins: Implications for Occupational and Public Health
by Chitra Narayanan and Yevgen Nazarenko
Atmosphere 2025, 16(8), 919; https://doi.org/10.3390/atmos16080919 - 29 Jul 2025
Viewed by 281
Abstract
Combustion of aviation jet fuel emits a complex mixture of pollutants linked to adverse health outcomes among airport personnel and nearby communities. While epidemiological studies showed the detrimental effects of aviation-derived air pollutants on human health, the molecular mechanisms of the interactions of [...] Read more.
Combustion of aviation jet fuel emits a complex mixture of pollutants linked to adverse health outcomes among airport personnel and nearby communities. While epidemiological studies showed the detrimental effects of aviation-derived air pollutants on human health, the molecular mechanisms of the interactions of these pollutants with cellular biomolecules like proteins that drive the adverse health effects remain poorly understood. In this study, we performed molecular docking simulations of 272 pollutant–protein complexes using AutoDock Vina 1.2.7 to characterize the binding strength of the pollutants with the selected proteins. We selected 34 aviation-derived pollutants that constitute three chemical categories of pollutants: volatile organic compounds (VOCs), polyaromatic hydrocarbons (PAHs), and organophosphate esters (OPEs). Each pollutant was docked to eight proteins that play critical roles in endocrine, metabolic, transport, and neurophysiological functions, where functional disruption is implicated in disease. The effect of binding of multiple pollutants was analyzed. Our results indicate that aliphatic and monoaromatic VOCs display low (<6 kcal/mol) binding affinities while PAHs and organophosphate esters exhibit strong (>7 kcal/mol) binding affinities. Furthermore, the binding strength of PAHs exhibits a positive correlation with the increasing number of aromatic rings in the pollutants, ranging from nearly 7 kcal/mol for two aromatic rings to more than 15 kcal/mol for five aromatic rings. Analysis of intermolecular interactions showed that these interactions are predominantly stabilized by hydrophobic, pi-stacking, and hydrogen bonding interactions. Simultaneous docking of multiple pollutants revealed the increased binding strength of the resulting complexes, highlighting the detrimental effect of exposure to pollutant mixtures found in ambient air near airports. We provide a priority list of pollutants that regulatory authorities can use to further develop targeted mitigation strategies to protect the vulnerable personnel and communities near airports. Full article
(This article belongs to the Section Air Quality and Health)
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27 pages, 2012 KiB  
Article
Dual Effects of Maternal Diet and Perinatal Organophosphate Flame Retardant Treatment on Offspring Development, Behavior and Metabolism
by Ali Yasrebi, Catherine M. Rojas, Shabree Anthony, Samantha Feltri, Jamilah Evelyn, Kimberly Wiersielis, Samantha Adams, Veronia Basaly, Grace L. Guo, Lauren M. Aleksunes and Troy A. Roepke
Toxics 2025, 13(8), 639; https://doi.org/10.3390/toxics13080639 - 29 Jul 2025
Viewed by 270
Abstract
The maternal–fetal environment is influenced by multiple factors, including nutrition and environmental contaminants, which can impact long-term development. Perinatal exposure to organophosphate flame retardants (OPFRs) disrupts energy homeostasis and causes maladaptive behaviors in mice. Maternal obesity affects development by impairing blood–brain barrier (BBB) [...] Read more.
The maternal–fetal environment is influenced by multiple factors, including nutrition and environmental contaminants, which can impact long-term development. Perinatal exposure to organophosphate flame retardants (OPFRs) disrupts energy homeostasis and causes maladaptive behaviors in mice. Maternal obesity affects development by impairing blood–brain barrier (BBB) formation, influencing brain regions involved in energy regulation and behavior. This study examined the combined effects of maternal obesity and perinatal OPFR treatment on offspring development. Female mice were fed either a low-fat (LFD) or a high-fat diet (HFD) for 8 weeks, mated, and treated with either sesame oil or an OPFR mixture (tris(1,3-dichloro-2-propyl)phosphate, tricresyl phosphate, and triphenyl phosphate, 1 mg/kg each) from gestational day 7 to postnatal day 14. Results showed that both maternal diet and OPFR treatment disrupted blood–brain barrier integrity, energy balance, and reproductive gene expression in the hypothalamus of neonates. The expression of hepatic genes related to lipid and xenobiotic metabolism was also altered. In adulthood, LFD OPFR-treated female offspring exhibited increased avoidance behavior, while HFD OPFR-treated females demonstrated memory impairments. Metabolic assessments revealed decreased energy expenditure and nighttime activity in LFD OPFR-treated females. These findings suggest that maternal diet and OPFR treatment alter hypothalamic and liver gene expression in neonates, potentially leading to long-term metabolic and behavioral changes. Full article
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14 pages, 2156 KiB  
Article
Microbiota of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) by 16S rDNA Illumina Sequencing
by Afef Najjari, Chahnez Naccache, Nour Abdelkefi, Salma Djebbi, Amira Souii, Brahim Chermiti, Mourad Elloumi and Maha Mezghani Khemakhem
Microbiol. Res. 2025, 16(7), 163; https://doi.org/10.3390/microbiolres16070163 - 19 Jul 2025
Viewed by 287
Abstract
Bemisia tabaci (Aleyrodidae family) is one of the most damaging pests of numerous crops worldwide. Insecticides, namely pyrethroids and organophosphates, have long been the primary control tools against this pest, resulting in several resistance cases. In Tunisia, the two most damaging biotypes [...] Read more.
Bemisia tabaci (Aleyrodidae family) is one of the most damaging pests of numerous crops worldwide. Insecticides, namely pyrethroids and organophosphates, have long been the primary control tools against this pest, resulting in several resistance cases. In Tunisia, the two most damaging biotypes of B. tabaci, MEAM1-B and MED-Q, are sympatric, and more concerns about developing resistance keep rising due to the extensive use of insecticides. Here, we aimed to elucidate the molecular mechanism of resistance to pyrethroids and organophosphorus insecticides in two Tunisian populations of B. tabaci, collected respectively on Capsicum annuum and Lantana camara, and then determine the bacterial community associated with insecticide resistance and susceptible biotypes based on 16S rRNA Illumina sequencing. The results showed that the population collected on Capsicum annuum belonged to the MEAM1-B biotype with an insecticide resistance profile. In contrast, the population collected on the Lantana camara belonged to the MED-Q biotype with a sensitive profile. The bacterial communities of the two biotypes were predominantly structured by the Proteobacteria phylum and three genera, including Candidatus Portiera, the secondary facultative symbiont, and Hamiltonella, which were unevenly distributed between the two biotopes. Our results provide the first evidence for insecticide resistance alleles in Tunisian MEAM1-B populations and suggest an association between bacterial community composition within susceptible biotypes and insecticide resistance. Full article
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13 pages, 1243 KiB  
Article
A Tandem MS Platform for Simultaneous Determination of Urinary Malondialdehyde and Diphenyl Phosphate
by Gabriela Chango, Diego García-Gómez, Carmelo García Pinto, Encarnación Rodríguez-Gonzalo and José Luis Pérez Pavón
Int. J. Environ. Res. Public Health 2025, 22(7), 1130; https://doi.org/10.3390/ijerph22071130 - 17 Jul 2025
Viewed by 271
Abstract
This study presents an advanced analytical method for the simultaneous quantification of malondialdehyde (MDA), a biomarker of oxidative stress, and diphenyl phosphate (DPhP), a metabolite of the organophosphate flame retardant triphenyl phosphate (TPhP), in human urine. The method integrates hydrophilic interaction liquid chromatography [...] Read more.
This study presents an advanced analytical method for the simultaneous quantification of malondialdehyde (MDA), a biomarker of oxidative stress, and diphenyl phosphate (DPhP), a metabolite of the organophosphate flame retardant triphenyl phosphate (TPhP), in human urine. The method integrates hydrophilic interaction liquid chromatography (HILIC), a type of liquid chromatography suitable for polar compounds, for MDA separation, and an online restricted access material (RAM), a preconcentration column, for DPhP isolation, achieving high specificity and sensitivity. Validation with certified urine samples confirmed its robustness across diverse analyte concentrations and complex biological matrices. The optimized clean-up steps effectively minimized carryover, allowing for high-throughput analysis. Application to 72 urine samples revealed a significant positive correlation (ρ = 0.702, p-value = 1.9 × 10−7) between MDA and DPhP levels, supporting a potential link between oxidative stress and TPhP exposure. The subset analysis demonstrated a statistically significant moderate positive correlation in women (ρ = 0.622, p-value = 0.020), although this result should be interpreted with caution because of the limited sample size (N = 14). This method provides a powerful tool for biomonitoring oxidative stress and environmental contaminants, offering valuable insights into exposure-related health risks. Full article
(This article belongs to the Special Issue Research on Environmental Exposure, Pollution, and Epidemiology)
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20 pages, 3290 KiB  
Article
The Impact of High Urban Temperatures on Pesticide Residues Accumulation in Vegetables Grown in the Greater Accra Metropolitan Area of Ghana
by Joyce Kumah, Eric Kofi Doe, Benedicta Yayra Fosu-Mensah, Benjamin Denkyira Ofori, Millicent A. S. Kwawu, Ebenezer Boahen, Doreen Larkailey Lartey, Sampson D. D. P. Dordaa and Christopher Gordon
J. Xenobiot. 2025, 15(4), 103; https://doi.org/10.3390/jox15040103 - 2 Jul 2025
Viewed by 785
Abstract
This study investigates the effect of high urban land temperatures on pesticide residue (PR) accumulation in cabbage and lettuce and on public health in the Greater Accra Metropolitan Area (GAMA) in Ghana. A comparative toxicological analysis regarding the food system was conducted with [...] Read more.
This study investigates the effect of high urban land temperatures on pesticide residue (PR) accumulation in cabbage and lettuce and on public health in the Greater Accra Metropolitan Area (GAMA) in Ghana. A comparative toxicological analysis regarding the food system was conducted with 66 farmers across three land surface temperatures: low (Atomic, n = 22), moderate (Ashaiman, n = 22), and high (Korle-Bu, n = 22). Pesticide residue concentrations were assessed using an ANOVA to examine spatial variations across sites. The results indicate a strong correlation between high land surface temperatures and pesticide residue accumulation, with lettuce recording significantly (p < 0.05) higher PR levels than cabbage. Several pesticides, including carbendazim (CBZ), Imidacloprid (IMI), Thiamethoxam (TMX), and Chlorpyrifos (CHL), exceeded the maximum residue limits (MRLs) set by the World Health Organization (WHO) and the European Union (EU) at moderate and high-temperature sites. carbendazim was the dominant pesticide detected, with a concentration of 19.0 mg/kg in lettuce, which far exceeded its maximum residue limit (MRL) of 0.10 mg/kg across all study sites. Statistical analyses (PERMANOVA) confirmed that land surface temperatures and pesticide types significantly influenced the PR concentrations. Public health risk assessments indicate that children are more vulnerable to pesticide exposure than adults. The toxicity hazard quotient (THQ) for organophosphate pesticides, particularly CHL and Dimethoate (DMT), exceeded safe thresholds at moderate and high-temperature sites. Full article
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15 pages, 2650 KiB  
Article
The Impact of Tetraethyl Pyrophosphate (TEPP) Pesticide on the Development and Behavior of Danio rerio: Evaluating the Potential of Cork Granules as a Natural Adsorbent for TEPP Removal from Aqueous Environments
by Fernanda Blini Marengo Malheiros, Lorrainy Victoria Rodrigues de Souza, Angélica Gois Morales, Eduardo Festozo Vicente, Paulo C. Meletti and Carlos Alberto-Silva
Clean Technol. 2025, 7(3), 54; https://doi.org/10.3390/cleantechnol7030054 - 28 Jun 2025
Viewed by 422
Abstract
Toxicological studies of pesticides in animal models provide critical insights into their mechanisms of action, while adsorption strategies offer potential solutions for decontaminating polluted waters. We evaluated toxicity induced by tetraethyl pyrophosphate (TEPP), an organophosphate pesticide and AChE inhibitor, on zebrafish (Danio [...] Read more.
Toxicological studies of pesticides in animal models provide critical insights into their mechanisms of action, while adsorption strategies offer potential solutions for decontaminating polluted waters. We evaluated toxicity induced by tetraethyl pyrophosphate (TEPP), an organophosphate pesticide and AChE inhibitor, on zebrafish (Danio rerio) development and behavior, alongside the efficacy of wine cork granules as a natural adsorbent. TEPP exposure reduced embryo viability following an inverted U-shaped dose–response curve, suggesting non-monotonic neurodevelopmental effects, but did not alter developmental timing or morphology in survivors. In juveniles, TEPP increased preference for dark environments (33% vs. controls) and enhanced swimming endurance approximately 3-fold, indicating disrupted phototaxis and stress responses. Most strikingly, water treated with cork granules retained toxicity, increasing mortality, delaying embryogenesis, and altering behavior. This directly contradicts in vitro adsorption studies that suggested cork’s efficacy. These results demonstrate the high sensitivity of zebrafish to TEPP at nanomolar concentrations, which contrasts with in vitro models that require doses approximately 1000 times higher. Our findings not only highlight TEPP’s ecological risks but also reveal unexpected limitations of cork granules for environmental remediation, urging caution in their application. Full article
(This article belongs to the Special Issue Pollutant Removal from Aqueous Solutions by Adsorptive Biomaterials)
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20 pages, 2206 KiB  
Article
Application of Carbon Materials Derived from Nocino Walnut Liqueur Pomace Residue for Chlorpyrifos Removal from Water
by Milena Zlatković, Rialda Kurtić, Igor A. Pašti, Tamara Tasić, Vedran Milanković, Nebojša Potkonjak, Christoph Unterweger and Tamara Lazarević-Pašti
Materials 2025, 18(13), 3072; https://doi.org/10.3390/ma18133072 - 28 Jun 2025
Viewed by 448
Abstract
This study explores the use of carbon materials derived from Nocino walnut liqueur pomace residue for the removal of chlorpyrifos, a widely used organophosphate pesticide, from water. Carbon adsorbents were synthesized from young walnut biomass under different thermal and chemical treatment conditions, and [...] Read more.
This study explores the use of carbon materials derived from Nocino walnut liqueur pomace residue for the removal of chlorpyrifos, a widely used organophosphate pesticide, from water. Carbon adsorbents were synthesized from young walnut biomass under different thermal and chemical treatment conditions, and their structural and surface properties were characterized using BET analysis, FTIR, SEM-EDX, Boehm titration, and zeta potential measurements. The materials exhibited distinct textural and chemical features, including high surface areas and varied surface functionalizations. Batch adsorption studies revealed that the chlorpyrifos removal followed pseudo-second-order kinetics and was best described by the Freundlich and Langmuir isotherms, indicating a combination of pore filling and physisorption via π-π and van der Waals interactions. The highest adsorption capacity of 45.2 ± 0.2 mg g−1 was achieved at 30 °C. Thermodynamic analysis confirmed the process to be endothermic, spontaneous, and entropy-driven, with desolvation effects enhancing the performance at elevated temperatures. Dynamic filtration experiments validated the practical applicability of the materials, while moderate reusability was achieved through ethanol-based regeneration. These findings demonstrate the potential of walnut pomace-derived carbons as low-cost, renewable, and effective adsorbents for sustainable water decontamination. Full article
(This article belongs to the Section Carbon Materials)
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14 pages, 627 KiB  
Article
Concentrations, Compositions and Human Exposure Risks to Organophosphate Esters in Indoor Air from Various Microenvironments in Guangzhou, China
by Yunmei Cai, Maoyuan Xu, Minghui Ouyang, Yusheng Wu, Ruijie Wang, Kewen Zheng and Guofa Ren
Toxics 2025, 13(7), 531; https://doi.org/10.3390/toxics13070531 - 25 Jun 2025
Viewed by 345
Abstract
Limited research has characterized the occurrence of organophosphate esters (OPEs) in indoor microenvironment air. To address this gap, ten OPE congeners were measured in air samples collected from 46 homes, 12 offices, 6 student dormitories, and 60 private cars in Guangzhou, China. Among [...] Read more.
Limited research has characterized the occurrence of organophosphate esters (OPEs) in indoor microenvironment air. To address this gap, ten OPE congeners were measured in air samples collected from 46 homes, 12 offices, 6 student dormitories, and 60 private cars in Guangzhou, China. Among the four microenvironments, private vehicles exhibited the highest total OPE concentrations (ΣOPEs), with an average of 264.89 ng/m3—statistically significantly higher than the other three environments (p < 0.05). This finding underscores the need for increased attention to OPE environmental fate in vehicles and associated human exposure risks. Distinct compositional profiles of OPEs were observed across microenvironments. In homes, offices, and student dormitories, tris(2-chloroethyl) phosphate (TCEP) and tris(2-chloropropyl) phosphate (TCPP) dominated the OPE mixture, accounting for 56% and 34% of ΣOPEs, respectively. By contrast, private cars were characterized by elevated levels of TCPP (68% of ΣOPEs) and tris(1,3-dichloro-2-propyl) phosphate (TDCP, 12%), reflecting source-specific emission patterns related to automotive materials. Significant correlations existed in most of the OPEs in the private cars, indicating that there are many potential sources of OPEs in private cars, and one source may release multiple OPEs. Human inhalation exposure to OPEs was estimated based on measured air concentrations. Daily respiratory exposure doses ranged from 9.1 to 30.85 ng/kg/d across different populations, with all values falling below established thresholds for non-carcinogenic and carcinogenic risks. These results indicate that current indoor air OPE levels in the studied microenvironments do not pose significant health hazards via inhalation pathways under typical exposure scenarios. Full article
(This article belongs to the Special Issue Environmental Transport and Transformation of Pollutants)
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18 pages, 339 KiB  
Review
Drug-Induced Autoimmune Hepatitis: Robust Causality Assessment Using Two Different Validated and Scoring Diagnostic Algorithms
by Rolf Teschke, Axel Eickhoff and Gaby Danan
Diagnostics 2025, 15(13), 1588; https://doi.org/10.3390/diagnostics15131588 - 23 Jun 2025
Viewed by 579
Abstract
Drug-induced autoimmune hepatitis (DIAIH) is a relatively new subtype of idiosyncratic drug-induced liver injury (iDILI), but the features of DIAIH have been variably described due to the inhomogeneity of assessed study cohorts. The aim of this analysis is to harmonize DIAIH cohorts by [...] Read more.
Drug-induced autoimmune hepatitis (DIAIH) is a relatively new subtype of idiosyncratic drug-induced liver injury (iDILI), but the features of DIAIH have been variably described due to the inhomogeneity of assessed study cohorts. The aim of this analysis is to harmonize DIAIH cohorts by unifying causality assessments, which may help characterize the features of DIAIH. Methods: Published reports of DIAIH cases were evaluated for the causality assessment methods used to verify the diagnosis of DIAIH. This disorder consists of two parts, i.e., the iDILI part and the autoimmune (AIH) part, whereby each part needs a specific diagnostic algorithm. The validated and scoring Roussel Uclaf Causality Assessment (RUCAM) is privileged for assessing the iDILI part, and the validated, simplified AIH score is the perfect choice for evaluating the AIH part. The analysis of DIAIH publications revealed that 12/20 reports (60%) presented cases assessed by both the RUCAM and the simplified AIH score, providing 49 drugs and drug combinations as causative drugs in up to 25 cases of DIAIH. Serum alanine aminotransferase activities of up to 3489 UL and high titers of autoimmune parameters such as anti-nuclear antibodies, anti-smooth-muscle antibodies, and soluble liver antigen antibodies supported DIAIH diagnosis. In contrast, 4/20 reports (20%) applied only RUCAM, and 2/20 reports (10%) used only the simplified AIH score; these 6 reports therefore provided insufficient criteria for a valid DIAIH diagnosis. Moreover, 2/20 reports (10%) did not use any causality algorithm, providing elusive features of DIAIH. While DIAIH is clearly restricted to drugs as responsible agents, this term is erroneously used to refer to disease induced by non-drugs such as herbs, green tea, dimethoate (an organophosphate insecticide), dietary supplements, biologics, herbal remedies, different viruses, and bacteria, as well as vaccines. For diseases induced by these agents, a better term could be, for instance, non-drug-induced autoimmune hepatitis. Drug cessation and immunotherapy with corticosteroids and azathioprine comprise the treatment of choice. The characteristics of DIAIH can best be described if both the RUCAM and the simplified AIH score are used concomitantly. Full article
(This article belongs to the Special Issue Diagnosis and Management of Liver Diseases—2nd Edition)
13 pages, 3435 KiB  
Article
Transcriptomic Analysis of Tambaqui (Colossoma macropomum) Exposed to Trichlorfon-Induced Toxicity
by Hallana Cristina Menezes da Silva, Igor Kelvyn Cavalcante Lobo, André Gentil da Silva, Ana Lúcia Silva Gomes, Wallice Paxiúba Duncan, Juliana Costa Silva, Fabrício M. Lopes, Roberto Ferreira Artoni and Daniele Aparecida Matoso
Animals 2025, 15(12), 1807; https://doi.org/10.3390/ani15121807 - 19 Jun 2025
Viewed by 338
Abstract
Trichlorfon is an antiparasitic agent widely used to control pests and parasites in farmed fish. Tambaqui (C. macropomum) is the most commercially important characid species farmed in the Amazon region. Trichlorfon exposure is known to cause physiological damage in fish due [...] Read more.
Trichlorfon is an antiparasitic agent widely used to control pests and parasites in farmed fish. Tambaqui (C. macropomum) is the most commercially important characid species farmed in the Amazon region. Trichlorfon exposure is known to cause physiological damage in fish due to its organophosphate nature. In this study, we used RNA-Seq to investigate the hepatic response of tambaqui following exposure to 0.435 mg/L of trichlorfon. The analysis revealed activation of several metabolic pathways, particularly those related to tumor processes, immune responses, and apoptosis. Additionally, we identified upregulation of solute carrier (SLC) genes, which may facilitate trichlorfon entry into hepatocytes. These findings enhance our understanding of fish responses to antiparasitic agents and support further research into the molecular impacts of organophosphate compounds in aquaculture species. Full article
(This article belongs to the Special Issue Ecotoxicology in Aquatic Animals: 2nd Edition)
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17 pages, 2269 KiB  
Article
Organophosphate Flame Retardants in Indoor Dust in the Tampa Bay (Florida) Area
by Adebayo Solanke, Lukasz Talalaj, Claire Graham and Henry Alegria
Toxics 2025, 13(6), 508; https://doi.org/10.3390/toxics13060508 - 16 Jun 2025
Viewed by 475
Abstract
As polybrominated diphenyl ethers were phased out as flame retardants and plasticizers, increasing quantities of organophosphate triesters (OPEs) have been used as replacements. Despite a surge in reports on levels and profiles of OPEs, especially in indoor environments, and the potential exposure, there [...] Read more.
As polybrominated diphenyl ethers were phased out as flame retardants and plasticizers, increasing quantities of organophosphate triesters (OPEs) have been used as replacements. Despite a surge in reports on levels and profiles of OPEs, especially in indoor environments, and the potential exposure, there are still understudied areas with no data on the levels of these chemicals. We carried out the first study investigating levels and profiles of OPEs in indoor dust from such an area, the Tampa Bay (Florida) area. ∑13OPEs measured at each site ranged from 545 to 502,086 ng g−1, with overall medians and means over 64 sites of 15,447 and 36,135 ng g−1, respectively. Alkyl OPEs were predominant, with lesser levels of chlorinated and aryl OPEs. Median levels were highest for tris (2-butoxyethyl) phosphate (TBOEP) and triphenyl phosphate (TPHP) at 4641 and 1046 ng g−1, respectively; lower for tris(1,3–dichloro-2-propyl) phosphate (TDCIPP), tris(2-chloropropyl) phosphate (T2CPP), and tris (2-chloroisopropyl) phosphate (TCIPP) at 530, 458, and 360 ng g−1, respectively; with others ranging from 2 to 85 ng g−1. There were differences in levels in different microenvironments (urban versus suburban; non-residential versus residential; apartments versus single-family homes; daycares versus residences and university rooms; building age; and rooms with different floor material). Estimated daily intakes for median and higher exposure scenarios for ∑13OPEs (in ng kg−1 bw day−1) were 12 and 552 for toddlers and 6 and 451 for adults, respectively. TBOEP accounted for 30% of total intake for toddlers and adults in a mean exposure scenario but 90% for high exposure scenario. Full article
(This article belongs to the Section Emerging Contaminants)
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14 pages, 998 KiB  
Article
The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
by Ramona Alina Tomuța, Andrada Florina Moldovan, Loredana Matiș, Lavinia Maris, Timea Claudia Ghitea and Florin Banica
Toxics 2025, 13(6), 504; https://doi.org/10.3390/toxics13060504 - 15 Jun 2025
Viewed by 528
Abstract
(1) Background: Exposure to pesticide residues through food remains a critical issue in public health, especially given their potential cumulative neurotoxic effects. (2) Methods: This study investigated the presence of pesticide residues in commonly consumed vegetables, fruits, and cereals based on official laboratory [...] Read more.
(1) Background: Exposure to pesticide residues through food remains a critical issue in public health, especially given their potential cumulative neurotoxic effects. (2) Methods: This study investigated the presence of pesticide residues in commonly consumed vegetables, fruits, and cereals based on official laboratory reports and evaluated the intestinal microbiome profiles of individuals whose diets consisted of over 50% plant-based foods. (3) Results: Analytical results from accredited laboratories in Romania demonstrated that all tested food samples were compliant with European regulations (Regulation (EC) 396/2005), with either undetectable or below-quantification-limit pesticide residues. However, organophosphates such as chlorpyrifos and diazinon were frequently tested, indicating persistent regulatory concern due to their known neurotoxic potential. A parallel analysis of stool samples revealed significant imbalances in neuroactive gut bacteria, including consistently low levels of Bifidobacterium and Lactobacillus species, and elevated levels of Oscillibacter and Alistipes, which are implicated in modulating GABA and serotonin pathways. Markers of proinflammatory activity, such as LPS-positive bacteria and histamine producers, were also elevated. (4) Conclusions: These findings suggest that even in diets rich in plant-based foods, microbial dysbiosis with neuroactive relevance can occur, potentially linked to environmental or dietary factors. The study underscores the need for a comprehensive evaluation of food safety and microbiome function as interconnected determinants of neurological health. Full article
(This article belongs to the Section Neurotoxicity)
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15 pages, 2003 KiB  
Article
Enhancing the Detection and Identification Sensitivity of Organophosphorus Pesticide-Related Phenols via Derivatization and LC-ESI-MS/MS: A Straightforward Approach to Identify the Specific Pesticide Involved in Exposure
by Avi Weissberg, Tamar Shamai Yamin, Avital Shifrovitch, Adi Tzadok, Merav Blanca and Moran Madmon
Environments 2025, 12(6), 193; https://doi.org/10.3390/environments12060193 - 8 Jun 2025
Viewed by 645
Abstract
Organophosphorus (OP) pesticides are a class of chemicals that are extensively used worldwide. The exposure to and use of organophosphates can be assessed by analyzing their metabolites and degradation products, such as dialkyl phosphate (DAP), dialkyl thiophosphate (DATP), and dialkyl dithiophosphate (DADTP). However, [...] Read more.
Organophosphorus (OP) pesticides are a class of chemicals that are extensively used worldwide. The exposure to and use of organophosphates can be assessed by analyzing their metabolites and degradation products, such as dialkyl phosphate (DAP), dialkyl thiophosphate (DATP), and dialkyl dithiophosphate (DADTP). However, since these metabolites/hydrolysis products can result from the metabolism or breakdown of several organophosphorus pesticide families, they serve as nonspecific biomarkers and do not indicate the specific pesticide involved in exposure. In an earlier study, chemical derivatization using N-(2-(bromomethyl)benzyl)-N,N-diethylethanaminium bromide (CAX-B) was described to improve the signal intensity of numerous organophosphorus (OP) acids in liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS) analysis. In the present study, CAX-B was employed to derivatize a set of seven phenolic compounds corresponding to the complementary portion of OP pesticides. The derivatization process using CAX-B was performed in acetonitrile with potassium carbonate at 50 °C for 30 min. LC-Orbitrap-ESI-MS/MS was used to analyze the resulting phenol derivatives and their fragmentation patterns were studied. Notably, the derivatized phenols were markedly more sensitive than the underivatized phenols when LC-ESI-MS/MS was used in MRM technique, without being affected by the sample matrix (soil or plant extracts). This derivatization technique aids in identifying OP pesticides, offers insights into their subfamily, and pinpoints a specific compound through the analysis of corresponding phenol derivative. Full article
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