Search Results (152)

Growing consumer demand for organic products and increasing evidence that viticultural management influences wine quality have prompted renewed interest in ecological cultivation systems. This study aimed to comparatively evaluate the oenological characteristics and sensory quality of wines produced from three Vitis vinifera varieties (Chardonnay, Sauvignon blanc, and Fetească neagră) grown under ecological and conventional cultivation systems in the Dealu Mare wine region (Romania) during 2022. Wines were characterized by their physicochemical parameters (pH, total acidity, reducing sugars, and ethanol content), total anthocyanin (TAC), total phenolic (TPC), and total flavonoid content (TFC), in vitro antioxidant activity (ABTS, DPPH, FRAP), and structured sensory evaluation using a semi-naive consumer panel (n = 20). Fetească neagră wines exhibited the highest bioactive potential, with TPC values up to 2895.78 ± 152.23 mg GAE/L, TAC up to 370.98 mg MGE/L, and FRAP values reaching 5790.53 ± 143.82 mg TE/L, approximately ten-fold higher than white wines. Ecological cultivation significantly enhanced the antioxidant activity of Sauvignon blanc and Fetească neagră wines, while its effect on Chardonnay was variable across assays. Sensory evaluation revealed that ecological cultivation significantly improved olfactory scores (p = 0.011), particularly for Sauvignon blanc, and that female panelists assigned significantly higher olfactory scores than males (p < 0.001). These findings support ecological viticulture as a quality-enhancing strategy for Romanian wine production, especially for autochthonous cultivars. Full article

Crop residues can harbor pathogens, making winter sanitation essential for sustainable viticulture. The grass–sheep–grape system could improve vineyard health through microbial optimization. To evaluate this, we assessed the effects of sheep feeding on fallen leaves on the occurrence of grape diseases through greenhouse experiments and used high-throughput-sequencing to compare microbial communities in grape fallen leaves and sheep feces, aiming to determine whether winter grazing reduces residue-borne pathogens. The results revealed that sheep grazing in vineyards significantly reduces the occurrence of grape leaf and cluster diseases, as well as a fundamental difference in microbial structures between leaves and feces, with no fungal taxa detected in the feces. The number of shared bacterial OTUs was minimal, while feces contained significantly more unique bacterial OTUs than fallen leaves. Additionally, bacterial diversity was significantly higher in feces than in fallen leaves. Sheep feces harbored a substantial number of highly efficient cellulose-degrading anaerobic bacteria, which may enhance organic matter conversion efficiency, and promote nutrient cycling in vineyards. Moreover, the grazing process directly reduced several pathogenic fungi associated with grape leaf, fruit, and root diseases. Functional analysis further indicated that fecal bacterial communities were primarily enriched in core metabolic and genetic processing functions, while leaf microbes were more involved in microbial interactions and secondary metabolism. More importantly, no function guilds of plant pathogenic fungi were present in feces. Overall, winter sheep grazing in vineyards can remove fallen leaves, not only reducing the risk of pathogen transmission but also potentially introducing beneficial bacterial communities. This study provides a feasible strategy for organic vineyard management in winter, and offers important insights for promoting sustainable vineyard production. Full article

The global pollinator decline is linked to intensive farming and the high use of plant protection products (PPPs), necessitating risk assessment and mitigation. This study investigates the potential negative impacts of agricultural practices on pollinator health, specifically focusing on the effects of PPPs used in viticulture on the honey bee, Apis mellifera, despite grapevines’ lack of reliance on bee pollination. The beehives sampled were from two farms with vineyards under different management regimes: one transitioning from conventional to organic practices and an organic–biodynamic site with pollinator mitigation measures. Sampling was conducted during three phases, pre-, during, and post-PPP application, to evaluate biomarkers of neurotoxicity (AChE), detoxification enzymes (CaE, GST), metabolic stress (ALP), and immune markers (Lys, PO, proPO). Comparison between the organic–biodynamic farm and the transitioning one revealed a pattern suggesting significant neurotoxic effects in the transitioning farm characterised by a trend of decreased AChE activity during treatments and the subsequent induction of GST post exposure. Crucially, both PO and proPO were induced post treatment, but with a lower PO/proPO ratio compared to previous seasons, suggesting inefficient proPO activation and potentially weakened immune competence that could favour pathogen proliferation. Bee health appeared to deteriorate most at the transitioning farm post treatment, while the biodynamic site remained relatively stable; these differences are likely associated with legacy residues and drift, exacerbated by overwintering stress and summer heat. Given the specific environmental and management characteristics of these two farms, the results provide an indicative comparison of how different agronomic approaches may influence bee health. Moreover, these results support the multi-biomarker approach for detecting potential PPP impacts, suggesting that organic transitions and mitigation strategies could play a role in pollinator conservation. Full article

Botrytis cinerea, the causal agent of grey mold in grapevine, remains one of the most economically important pathogens in viticulture and a key target for sustainable biocontrol strategies. This study evaluated the antagonistic potential of seven Trichoderma isolates (T1–T7), collected from the rhizosphere of grapevine in Morocco, using a combination of in vitro and in planta assays designed to capture multiple direct and indirect modes of action. The isolates exhibited variable levels of antagonism through competition, volatile organic compounds, extracellular metabolites, and elicitation responses. Preliminary in planta assays on detached grape berries further demonstrated that all selected isolates reduced lesion development, with preventive applications yielding the strongest protection. Overall, the study highlights the complementary and strain-specific mechanisms underlying Trichoderma & B. cinerea interactions and underscores the importance of isolate selection and application timing for the development of effective and environmentally friendly grey mold management strategies. These findings provide a mechanistic basis for the future evaluation of promising isolates under vineyard conditions. Full article

Recycling vineyard pruning residues into compost and vermicompost represents a sustainable strategy to reduce viticulture’s reliance on chemical fertilizers. Nonetheless, their effects on plant performance remain poorly understood. This study evaluated the effect of vine pruning residues compost and vermicompost on the physiological, biochemical, and growth performance of Vitis vinifera L. cv. Touriga Franca, in comparison with mineral fertilization and an unfertilized control. A pot experiment was conducted from April to September 2024 in northern Portugal under Mediterranean climate conditions, using one-year-old grapevines and subjected to four fertilization treatments. Leaf gas exchange, chlorophyll a fluorescence, photosynthetic pigments, antioxidant and osmoprotective metabolites, and shoot and root development were assessed at three sampling dates during the growing season. Organic amendments enhanced photosynthetic performance and root growth relative to the unfertilized control. Vermicompost promoted higher CO₂ assimilation, stomatal conductance, and shoot and root elongation, whereas compost increased intrinsic water use efficiency, photochemical regulation, and root biomass. Biochemical analyses indicated that compost favored protein and carotenoid accumulation, while vermicompost increased proline and later protein levels, alongside reduced phenolic and flavonoid contents. Despite their similar chemical composition, compost and vermicompost induced distinct physiological responses driven by differences in biological activity and nutrient dynamics. These findings demonstrate that pruning-derived organic amendments can match mineral fertilization in supporting grapevine performance while offering additional benefits for stress regulation and sustainable vineyard management. Full article

Globalization, climate change and the energy crisis are having a negative impact on the agricultural sector. Moreover, in Greece, producers need to invest in modernizing their farms, introducing innovations, achieve digital transformation and increase productivity. Incentives are available for green investments, thus facilitating the adoption of innovations. This paper examines the attitudes and perceptions of winegrowers in Western Macedonia regarding environmental protection and organic farming. Specifically, with the help of the k-means algorithm, respondents are divided into two groups. The first group consists of producers who have a negative attitude towards environmental issues and have a lower level of education, while the second group consists of those who have a positive attitude. In the first group in particular, most respondents do not seem to have a good knowledge of environmental protection issues, believe that cross-compliance is very stringent and do not have a high level of education. This means that policy measures must be taken to adequately inform and educate farmers belonging to this group to achieve the goal of environmental protection. The results of the second group include producers with a positive attitude, who are expected to play an important role in the development of sustainable viticulture by creating conditions for a better future at an individual, regional and social level. Full article

Volatile organic compounds (VOCs) produced by Medicinal Aromatic Plants (MAPs) are bioactive signaling molecules that play key roles in plant defense, acting against pathogens and triggering resistance responses. Intercropping with VOC-emitting MAPs can therefore enhance disease resistance. This study investigated VOCs emitted by sage (Salvia officinalis) as potential resistance inducers in grapevine (Vitis vinifera) against Plasmopara viticola, the causal agent of downy mildew, under consociated growth conditions. Sage and grapevine plants were co-grown in an airtight box system for 24 or 48 h, after which grape leaves were inoculated with P. viticola. Disease assessments were integrated with grapevine leaf metabolic profiling to evaluate responses to VOC exposure and pathogen infection. Untargeted and targeted metabolomic analysis revealed that sage VOCs consistently reprogrammed grapevine secondary metabolism, without substantial differences between 24 and 48 h exposures. Lipids, phenylpropanoids, and terpenoids were markedly accumulated following VOC exposure and persisted following inoculation. Correspondingly, leaves pre-exposed to sage VOCs exhibited a significant reduction in disease susceptibility. Overall, our results suggest that exposure to sage VOCs induces signaling and metabolic reprogramming in grapevine. Further research should elucidate how grapevines perceive and integrate these signals, as well as the broader processes underlying MAP VOC-induced defense, and evaluate their translation into sustainable viticultural practices. Full article

This study examined how different fertilisation strategies (mineral, compost, vermicompost and non-fertilised control) influence vine nutrient status, must composition and wine chemical characteristics over two consecutive seasons (2024–2025) in two semi-arid Mediterranean vineyards, one deficit-irrigated and other rainfed. Compost and vermicompost were produced from winery residues, in line with a circular management approach. Organic fertilisation improved vine nitrogen and potassium levels, particularly at veraison, with cumulative effects observed over time. Musts from fertilised vines (mineral, compost and vermicompost) exhibited higher levels of yeast-assimilable nitrogen (YAN) and pH, as well as lower titratable acidity, compared to the control group (without fertilization). Wines obtained from these treatments exhibited higher ethanol content and modified acidity parameters, with compositional changes being more evident in the rainfed vineyard. Analysis of volatile compounds revealed that organic fertilisers, particularly vermicompost, promoted the formation of esters, higher alcohols, and terpenes linked to grape metabolism and fermentation. These results demonstrate that organic amendments derived from winery waste can serve as efficient nutrient sources, thereby enhancing the nutritional balance of vines and the composition of wines, while also promoting sustainable and circular practices in viticulture. Full article

Scaphoideus titanus Ball, 1932 (Hemiptera: Cicadellidae), the main vector of the phytoplasma (FDp) causing Flavescence dorée, is considered a challenging problem in European vineyards. Natural insecticides permitted for S. titanus control in Italian organic viticulture are generally considered of low-to-moderate efficacy. Morever, it should be mentioned that their optimal use requires a careful approach when preparing the insecticide solutions. Synthetic insecticides used in Italian viticulture are included in only two IRAC groups, indicating potential risks for resistance. Therefore, there is a need to identify alternatives or control tools complementary to synthetic insecticides. In this study, we summarize the results obtained in five field experiments (2021–2025) using the main alternatives to synthetic insecticides (i.e., pyrethrins, kaolin, azadirachtin, Beauveria bassiana, and potassium salts of fatty acids). In all seasons, pre-insecticide application populations did not differ significantly among treatments, whereas differences emerged after insecticide applications. The overall results showed that pyrethrin-based insecticides were the most effective in reducing S. titanus nymph densities. Kaolin provided intermediate but comparatively stable levels of control across the years, whereas B. bassiana showed moderate yet highly variable efficacy. The findings obtained here can be considered in designing management strategies for S. titanus in organic viticulture that require an integrated approach combining pyrethrins with complementary, lower-impact tools to achieve effective and sustainable control over time. At the same time, natural insecticides can be incorporated into IPM strategies in conventional vineyards to prevent resistance. Full article

Climate change and unsustainable agricultural practices are triggering land degradation in semi-arid Mediterranean regions. Organic amendments, such as mulching materials, have shown promising potential to mitigate these impacts by improving soil chemical, physical, and biological properties, while enhancing grapevine growth and productivity. This study evaluated the effects of wheat straw mulch (M) and wheat straw combined with biochar (MB), together with vineyard topography (bottom vs. top), on grape chemical and phenolic composition in four Vitis vinifera L. cultivars (Syrah, Trincadeira, Alicante Bouschet, and Antão Vaz) grown in the Alentejo wine region. Grapes were sampled separately at top and bottom topographic positions, and classical and phenolic parameters were analyzed. The application of M and MB significantly modified must composition, mainly through changes in nitrogen and sugar levels across topographic positions. Only MB exhibited stronger effects, enhancing must quality, while MB and M reduced bottom–top variability. Similar patterns and positional effects were observed for phenolic and color parameters. Both organic treatments lowered total monomeric anthocyanin concentrations, although positional differences with wheat straw mulch were found. The results highlight that combining soil management with topography and variety response can optimize grape phenolic composition and promote sustainable viticulture through targeted, site-specific mulching strategies. Full article

The winemaking industry represents one of the most important sectors of the Mediterranean agrifood economy, generating large amounts of solid residues, especially grape pomace. The study aimed to evaluate during two consecutive harvest years the influence of the production system (conventional vs. organic) and cultivar on the mineral, chemical, and antioxidant composition, as well as the colorimetric properties, of grape pomaces obtained from four Vitis vinifera L. cultivars in Alentejo-Portugal. The results showed that mineral composition was significantly affected by both production system and cultivar, with organic grape pomace showing higher K and Mn contents, whereas Ca and Cu showed consistently higher content under conventional. Protein content tended to increase under organic production, while fiber and fat were overall higher in conventional, particularly in the first year. Sugars displayed strong cultivar specificity, with Arinto showing the highest concentrations (30 to 40%), and considering all cultivars, total phenolic content ranged between 4000 ando 9000 mg GAE/100 g, while antioxidant capacity varied among cultivars and years. Colorimetric parameters were essentially influenced by cultivar and harvest year rather than production system. The PCA revealed that PC1 (44.06%) represented a gradient associated with mineral and antioxidant composition, while PC2 (21.26%) reflected minor variation in color and sugars, and the hierarchical clustering distinguished Syrah and Alicante Bouschet as the cultivars most responsive to production system, whereas Aragonez and Arinto exhibited greater compositional stability across years. Overall, the findings indicate that both cultivar and management practices (organic and conventional) influence the compositional profile of grape pomace, with organic showing a tendency to enhance K, Mn, protein, and antioxidant parameters, whereas conventional practices favored higher levels of Ca, Cu, and fiber. The results provide valuable insights for the valorization of grape pomace and the development of sustainable viticultural strategies in Mediterranean environments. Full article

Botrytis cinerea Pers., the causal agent of grey mould, causes major economic losses in viticulture by reducing grape and wine quality and yield. Antagonistic yeasts that release bioactive volatile organic compounds (VOCs) represent a sustainable alternative to synthetic fungicides. Here, VOCs produced by Pichia guilliermondii strain ZIM624 were identified and assessed for antifungal activity against B. cinerea. 65 VOCs—including higher alcohols, volatile phenols, esters, and terpenes—were detected using two newly developed and validated analytical methods combining automated headspace solid-phase microextraction with gas chromatography–mass spectrometry. A total of 13 VOCs were selected for the bioassays. Fumigation assays demonstrated that terpenes (citronellol, geraniol, nerol, α-terpineol, and linalool) were the most effective inhibitors of B. cinerea mycelial growth (EC₅₀ = 6.3–33.9 μL/L). Strong inhibition was also observed for 4-vinylphenol and isoamyl acetate. In vivo assays confirmed that exposing infected grape berries to P. guilliermondii VOCs significantly reduced grey mould incidence. These results highlight the potential of P. guilliermondii ZIM624 volatiles as natural biofumigants for the eco-friendly management of B. cinerea in grapes. Future research should focus on optimising VOC production, evaluating efficacy under field conditions, and developing formulations for practical application in vineyards and post-harvest storage. Additionally, investigating potential synergistic effects of VOC combinations could lead to more effective biocontrol strategies. Full article

Background: Viticulture in Southern Brazil heavily relies on fungicides, such as Mancozeb, to manage fungal diseases. Increasing concern has emerged regarding the chronic health effects of Mancozeb exposure among vineyard workers, particularly its potential to induce oxidative stress and genotoxicity. Methods: A cross-sectional study was conducted between July and November 2023 involving 94 participants: 50 vineyard workers occupationally exposed to Mancozeb and 44 organic farmers with no history of pesticide exposure, who served as the control group. Eligible participants were aged 18 years or older, and exposed individuals had at least 5 years of documented Mancozeb use. Data on demographics, health status, occupational history, and use of personal protective equipment (PPE) were collected through structured interviews. Blood and urine samples were analyzed to determine hematological and biochemical parameters, oxidative stress biomarkers, genotoxicity (via comet assay and micronucleus test), and urinary ethylene thiourea (ETU), the primary metabolite of Mancozeb. Results: Workers exposed to Mancozeb exhibited significantly elevated levels of oxidative stress markers (p < 0.001) and DNA damage in both genotoxicity assays (p < 0.001). Urinary ETU concentrations were also markedly elevated, and a threshold of 69.3 ng/mL was identified as a discriminative marker of exposure. Conclusions: This study offers a novel contribution by proposing a specific biological exposure limit for ETU concentrations, derived from ROC curve analysis, representing a significant advancement in occupational health. The findings underscore the urgent need for regulatory biological exposure limits and the implementation of effective preventive strategies. Full article

Climate change, driven largely by increasing atmospheric CO₂ from fossil fuel combustion and soil carbon losses caused by unsustainable land use, threatens agricultural productivity and ecosystem services. Viticulture, developed mostly in Mediterranean and temperate regions, is particularly vulnerable by rising temperatures, decreasing precipitation, and soil degradation. Nevertheless, vineyards also offer opportunities to mitigate climate change by increasing soil organic carbon (SOC) and plant biomass. This review analyzes current scientific evidence on the impact of soil management practices in vineyards. Implementing strategies such as the use of cover crops, organic amendments, the incorporation of pruning residues, and reduced tillage can significantly contribute to carbon sequestration and soil health. Sequestration rates vary widely, from 2.8 to more than 11 Mg CO₂ ha⁻¹ year⁻¹, depending on climate, soil type, and practices used. Average carbon sequestration rates for crops with minimum or reduced tillage range between 2.5 and 5 Mg CO₂ ha⁻¹ year⁻¹, increasing to 7 and 7.5 Mg CO₂ ha⁻¹ year⁻¹ when organic fertilizers are used. Uncertainties in the economic balance, initial costs, and weak political incentives hinder the adoption of sustainable agricultural strategies, highlighting the need for further research into expanding their application. These strategies also improve microbial activity, nutrient cycling, and resilience to abiotic stresses. Tailoring integrated approaches to local environmental conditions is essential to moving toward sustainable, resilient, and climate-responsible viticulture. Full article

Viticulture in insular and volcanic environments faces mounting pressures from land abandonment, limited mechanization, and climate-related stress on soil and water resources. This study develops an integrated framework combining Life Cycle Assessment (LCA) and soil diagnostics to evaluate the environmental and agronomic performance of vineyards on the island of Tenerife (Canary Islands, Spain). Fifteen representative vineyards located between 100 and 1000 m a.s.l. within the Tacoronte–Acentejo Denomination of Origin were assessed using the ReCiPe 2016 Midpoint (H) method and the Ecoinvent 3.8 database. The average carbon footprint reached 1.40 kg CO₂-eq kg⁻¹ of grapes, with diesel use for field access and transport contributing over 50% of total impacts and 64% of human toxicity. Copper-based fungicides accounted for ~11% of impacts, underscoring their environmental persistence. Soil analyses revealed widespread Ca/Mg imbalances and sporadic K deficiencies, while organic matter and pH levels were generally adequate. Importantly, vineyards with balanced nutrient ratios exhibited both higher yields and lower environmental burdens, suggesting that improved soil health can enhance eco-efficiency, primarily by supporting higher yields under similar input regimes. Targeted strategies—such as magnesium supplementation, reduced copper inputs, and low-carbon mobility practices—can therefore mitigate emissions while improving productivity. The proposed LCA–soil integration provides a replicable model for sustainable resource management and climate-resilient viticulture in other fragile and topographically constrained agricultural systems. Full article