Search Results (60)

Single-cell adhesion assays can be divided into studies on attachment and detachment events, and several methods that enable the characterization of both processes have been established in the past. Due to their low invasiveness, label-free principles, and contactless operation, optical methods are especially beneficial for this purpose. Historically, optical tweezers (OTs) have been used to explore single-cell detachment events, allowing for the precise determination of minute physical forces. However, it has been noted that OTs can also be used to study single-cell attachment dynamics, including the evaluation of minimum cell-to-cell contact times necessary to establish a stable adhesive bond. Here, we provide a step-by-step protocol to effectively evaluate minute changes in the adhesion of single leukemia–lymphoma cells using optical tweezers with low laser intensities. This serves as a valuable in vitro model to determine the effects of physical and chemical factors on the adhesive properties of leukemia–lymphoma (LL) cells. Full article

The research on high-order transverse modes in lasers is a subject as old as the laser itself and has been largely abandoned. However, recently several studies have demonstrated an interest in using, instead of the usual Gaussian beam, a radial Laguerre–Gauss LG_p0 beam, as, for instance, one can observe a strong improvement, for a given power, in the longitudinal and radial forces in optical tweezers illuminated by a LG_p0 beam instead of the usual Gaussian beam. Since in most commercial lasers, the delivered laser beam is Gaussian, we therefore think it opportune to consider the problems of forcing a laser to oscillate individually on a higher-order transverse LG_p0 mode. We propose a comprehensive analysis of the effects of an intra-cavity phase or amplitude mask on the fundamental mode of a plano-concave cavity. In particular, we discuss the best choice of parameters favouring the fundamental mode of a pure radial Laguerre–Gauss LG_p0 model. Full article

Nanoscale resonant devices based on optical tweezers are widely used in the field of precision sensing. In the process of driving the nanoresonator based on the Coulomb force, the real-time, precise regulation of the charge carried by the charged resonator is essential for continuous manipulation. However, the accuracy of the existing charge measurement methods for levitated particles is low, and these methods cannot meet the needs of precision sensing. In this study, a novel net charge measurement protocol for levitated particles based on spatial speed statistics is proposed. High-precision mass measurement based on Maxwell’s rate distribution law is the basis for improving the accuracy of charge measurement, and accurate measurement of net charge can be achieved by periodic electric field driving. The error of net charge measurement is less than 7.3% when the pressure is above 0.1 mbar, while it can be less than 0.76% at 10 mbar. This proposed method features real-time, high-precision, non-destructive, and in situ measurement of the net charge of particles in the medium vacuum, which provides new solutions for practical problems in the fields of high-precision sensing and nano-metrology based on levitated photodynamics. Full article

Transverse mode control of laser intracavity oscillation is crucial for generating high-purity cylindrical vector beams (CVBs). We utilized the mode conversion and mode selection properties of two-mode long-period fiber gratings (TM-LPFGs) and two-mode fiber Bragg gratings (TM-FBGs) to achieve intracavity hybrid-mode oscillations of LP₀₁ and LP₁₁ from an all-few-mode fiber laser. A mode-locked pulse output with a repetition rate of 12.46 MHz and a signal-to-noise ratio of 53 dB was achieved with a semiconductor saturable absorber mirror (SESAM) for mode-locking, at a wavelength of 1550.32 nm. The 30 dB spectrum bandwidth of the mode-locked pulse was 0.13 nm. Furthermore, a high-purity CVB containing radially polarized and azimuthally polarized LP₁₁ modes was generated. The purity of the obtained CVB was greater than 99%. The high-purity CVB pulses have great potential for applications in optical tweezers, high-speed mode-division multiplexing communication, and more. Full article

It is well established that the interaction of light with matter generally imparts mechanical forces and associated torques. Where laser light is involved, such effects can be deployed for the microscale and nanoscale manipulation of matter, giving rise to notable applications in wide areas of physics, chemistry, and the life sciences. The diversity of applications is enriched not only by the complexity of the constitution of matter, but also by the structure of the laser light itself, according to its polarisation, amplitude, and phase. This article reviews in comprehensive terms the origins of laser-based optical forces and their manifestations in a variety of current contexts and applications. Full article

Usually, optical tweezers for trapping atoms or nanoparticles are based on the focusing of a Gaussian laser beam (GB). The optical trap is characterised by its longitudinal stability (LS), expressed as the ratio of the backward axial gradient and the forward scattering forces. Replacing the GB with a LG_p0 beam (one central peak surrounded by p rings) does not improve the LS because the on-axis intensity distribution is the same whatever the mode order p. However, it has been recently demonstrated that a restructured LG_p0 beam can improve greatly the LS. In this paper, we consider the restructuring of a LG_p0 beam when passing through a simple binary diffracting optical element called a circular π-plate (CPP). For a particular radius of the dephasing zone of the CPP, it is found that the LS is multiplied by a factor corresponding to a relative increase of about 220% to 320%. Full article

Perfect vortex beams (PVBs) possess the advantage of a stable light field distribution regardless of their topological charges, and thus they are extensively utilized in various applications, such as free-space optical communication, optical tweezers and laser processing. Herein, we report a new strategy to generate and modulate PVBs using coherent beam combining (CBC) technology. Both piston phase and tilting phase controlling methods have been successfully employed, and the corresponding properties of the generated PVBs have been fully investigated. Moreover, the number and position of the gaps in fractional perfect vortex beams (FPVBs) could be precisely controlled, and the relationships between these modulated parameters and the performance of FPVBs are uncovered. These simulation analysis results demonstrate the potential for flexible modulation of PVBs or FPVBs in the CBC system, indicating promising prospects for coherent beam arrays (CBAs) in laser beam shaping and achieving high-power structured light. Full article

Using tight focusing light, optical tweezers (OT) are tools that can manipulate and capture microscopic particles and biological cells as well as characterize a wide range of micro and nanomaterials. In this paper, we focused on fibroblasts, which are widely used in the biomedical area for a variety of purposes, including promoting human wound healing and preventing the early proliferation of tumor cells. We first built an optical tweezer experimental platform, using an 808 nm continuous-wave laser as the capture light source, to confirm that the device can precisely control the movement of single or multiple particles as well as fibroblasts. Then, a 1030 nm femtosecond laser was employed as the capture light source to study the manipulation of microparticles and fibroblasts at different powers. Lastly, a protracted manipulation protocol was used to prevent the fibroblasts from adhering to the wall. This method can be used to isolate and precisely block adherent growth of fibroblasts in cell populations. This experimental result can be further extended to other biological cells. Full article

Background: Our study aimed to assess the radiation sensitivity of BT20, a human breast tumor cell line, using the laser-trapping technique and compare it with N2a and 4T1 cells. Additionally, we investigated the impact of the antitumor compound 2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD) on radiation sensitivity. Methods and Materials: We employed laser trapping to calculate both the threshold ionization energy (TIE) and threshold radiation dose (TRD) for BT20, N2a, and 4T1 cells. We assessed the effect of DMDD on BT20 cells’ radiosensitivity and conducted comparisons across these cell lines. Results: Our findings reveal that DMDD significantly enhances the radiosensitivity of BT20 breast carcinoma cells. Moreover, we observed distinct trends in TIE and TRD across the three cell lines, with differences attributed to variations in cell size and composition. When multiple cell ionizations were considered, a notable reduction in TRD was observed, implicating factors such as the chain effect of ionizing radiation and the influence of DMDD. The study found that TIE increased with the number of cells in the trap while TRD consistently decreased across all three cell lines, suggesting comparable radiation sensitivity, and oligostilbene treatment further reduced TRD, presenting the potential for enhancing therapeutic ratios in cancer treatment. Conclusion: The antitumor compound DMDD enhances the radiosensitivity of BT20 breast carcinoma cells, highlighting its potential in cancer treatment. Furthermore, our study underscores the impact of cell size and multiple-cell ionizations on TRD. Leveraging laser trapping techniques, biocompatible nanoparticles, and advanced optical tweezers opens promising avenues for personalized and effective cancer therapy approaches. Full article

Optical tweezers (OT), or optical traps, are a device for manipulating microscopic objects through a focused laser beam. They are used in various fields of physical and biophysical chemistry to identify the interactions between individual molecules and measure single-molecule forces. In this work, we describe the development of a homemade optical tweezers device based on a cost-effective IR diode laser, the hardware, and, in particular, the software controlling it. It allows us to control the instrument, calibrate it, and record and process the measured data. It includes the user interface design, peripherals control, recording, A/D conversion of the detector signals, evaluation of the calibration constants, and visualization of the results. Particular stress is put on the signal filtration from noise, where several methods were tested. The calibration experiments indicate a good sensitivity of the instrument that is thus ready to be used for various single-molecule measurements. Full article

Light beams bearing orbital angular momentum (OAM) are used in various scientific and engineering applications, such as microscopy, laser material processing, and optical tweezers. Precise topological charge control is crucial for efficiently using vortex beams in different fields, such as information encoding in optical communications and sensor systems. This work presents a novel method for optimizing an emitting micro-ring resonator (MRR) for emitting vortex beams with variable orders of OAM. The MRR consists of a ring waveguide with periodic structures side-coupled to a bus waveguide. The resonator is tunable due to the phase change material Sb₂Se₃ deposited on the ring. This material can change from amorphous to crystalline while changing its refractive index. In the amorphous phase, it is 3.285 + 0i, while in the transition to the crystalline phase, it reaches 4.050 + 0i at emission wavelength 1550 nm. We used this property to control the vortex beam topological charge. In our study, we optimized the distance between the bus waveguide and the ring waveguide, the bending angle, and the width of the bus waveguide. The optimality criterion was chosen to maximize the flux density of the radiated energy emitted by the resonator. The numerical simulation results proved our method. The proposed approach can be used to optimize optical beam emitters carrying OAM for various applications. Full article

Nanostructures and nanomaterials, especially plasmonic nanostructures, often show optical properties that conventional materials lack and can manipulate light, as well as various light–matter interactions, in both their near-field and far-field regions with a high efficiency. Thanks to these unique properties, not only can they be used to enhance the sensitivity of chemical sensing and analysis techniques, but they also provide a solution for designing new sensing devices and simplifying the design of analytical instruments. The earliest applications of optical nanostructures are surface-enhanced spectroscopies. With the help of the resonance field enhancement of plasmonic nanostructures, molecular signals, such as Raman, infrared absorption, and fluorescence can be significantly enhanced, and even single-molecule analysis can be realized. Moreover, the resonant field enhancements of plasmonic nanostructures are often associated with other effects, such as optical forces, resonance shifts, and photothermal effects. Using these properties, label-free plasmonic sensors, nano-optical tweezers, and plasmonic matrix-assisted laser desorption/ionization have also been demonstrated in the past two decades. In the last few years, the research on optical nanostructures has gradually expanded to non-periodic 2D array structures, namely metasurfaces. With the help of metasurfaces, light can be arbitrarily manipulated, leading to many new possibilities for developing miniaturized integrated intelligent sensing and analysis systems. In this review, we discuss the applications of optical nanostructures in chemical sensing and analysis from both theoretical and practical aspects, aiming at a concise and unified framework for this field. Full article

In this paper, an analytical method for studying the radiation force (RF) of chiral spheres generated by dual laser beams is presented under the framework of generalized Lorenz–Mie theory (GLMT). According to the coordinate transformation relations, the arbitrarily incident laser beam is represented by vector spherical harmonic functions (VSHFs) in the sphere system. The entire induced field expression coefficients of dual laser beams can be obtained by superposition of each illuminated field. Based on the momentum conservation theory, the concrete expression of lateral and axial RF on chiral sphere is derived. The current theories are shown to be valid by comparison with the existing reference. To investigate the stable capture state of chiral sphere, the influences of the corresponding parameters of chiral particles and dual laser beams on the trapping and manipulation are investigated in detail. The analytical study on the RF of dual laser beams on chiral particles is an efficient method for improving optical tweezers technology and can become an encouraging approach to realize the high accuracy operation of chiral particles. Full article

To form a diffraction-free beam with a complex structure, we propose to use a set of primitives calculated iteratively for the ring spatial spectrum. We also optimized the complex transmission function of the diffractive optical elements (DOEs), which form some primitive diffraction-free distributions (for example, a square or/and a triangle). The superposition of such DOEs supplemented with deflecting phases (a multi-order optical element) provides to generate a diffraction-free beam with a more complex transverse intensity distribution corresponding to the composition of these primitives. The proposed approach has two advantages. The first is the rapid (for the first few iterations) achievements of an acceptable error in the calculation of an optical element that forms a primitive distribution compared to a complex one. The second advantage is the convenience of reconfiguration. Since a complex distribution is assembled from primitive parts, it can be reconfigured quickly or dynamically by using a spatial light modulator (SLM) by moving and rotating these components. Numerical results were confirmed experimentally. Full article

Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 $\mathsf{\mu }$g mL${}^{-1}$ was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts. Full article