Search Results (21)

Apple (Malus domestica) is a deciduous perennial tree that belongs to the family “Rosaceae”. Due to the highly suitable agro-climatic conditions for apple cultivation, it is among the widely cultivated fruits in Gilgit-Baltistan (GB). This study aims to evaluate elite apple genotypes in GB based on morphological and biochemical traits. Five- to six-year-old plants were selected for this study. The research employed a completely randomized design (CRD) with three replications, and mean differences were analyzed using the LSD test. In this study, five genotypes were assessed based on morphological (fruit weight, fruit length, fruit width, and firmness) and biochemical attributes (TSS, TA, ascorbic acid, total phenolic content, and flavonoid content). The results revealed significant variation among apple cultivars in both phytochemical and fruit quality attributes. The cultivar ‘Red Delicious’ exhibited the highest fruit weight (146.18 g), total soluble solids (TSS) (15.4 °Brix), and flavonoid content (105.75 mg 100 g⁻¹ FW). In contrast, ‘Red Full Star’ demonstrated superior firmness (7.19 kg cm⁻²), along with the highest total phenolic content (TPC) (4.00 mg GAE g⁻¹ FW) and ascorbic acid content (26.45 mg 100 g⁻¹ FW). Although the indigenous variety ‘Nus Khushu’ exhibited lower values in commercial traits, it holds substantial potential for conservation due to its unique local adaptation. The findings indicate that the “Red Delicious” and “Red Full Star” cultivars cultivated in GB are enriched with a variety of bioactive compounds that offer notable health benefits and may be utilized for future crop enhancement and breeding initiatives. Full article

Nitrogen (N) deficiency in apples (Malus × domestica Borkh.) leads to characteristic physiological symptoms, including leaf and fruit discoloration. Fertigation, i.e., the application of dissolved fertilizers, can significantly improve the growth and fruit quality of apples while optimizing nutrient uptake through a more precise and better timed application than conventional fertilization. This study therefore investigates how different fertilization treatments affect the N concentration of different age categories of apple leaves. Apples of the variety ‘Braeburn’ were grown hydroponically on the low-vigorous rootstock M9. Four fertilizer treatments were used: (1) Hoagland solution (HS); (2) HS nitrogen excluded; (3) HS iron excluded; and (4) HS magnesium excluded. Through vegetation, leaf samples were taken from three shoot positions representing different leaf ages (young, semi-young and old) and then chemically analyzed. The lowest N concentrations across all leaf ages and sampling moments were found in the treatment with N excluded (1.69–2.07% N), while the highest values occurred in the treatments where iron (2.00–2.49% N) or magnesium (1.98–2.37% N) were excluded. The seasonal changes in N concentration reflect interactions between the leaf age and the sampling moment. These results show that the N concentration of apple leaves strongly depends on the type of fertilization. Full article

Apples (Malus domestica Borkh.) represent one of the most widely cultivated and consumed fruits globally, with significant genetic diversity among cultivars. This study aimed to evaluate the morphological, biochemical, and organoleptic characteristics of 34 apple cultivars, including ancient Romanian varieties, internationally old and modern cultivars, and new selections. The assessment was conducted to identify valuable traits for breeding programs and commercial applications. Morphological analysis revealed significant variation in fruit size, shape, and weight, with international ‘classic’ cultivars exhibiting larger dimensions on average. Biochemical profiling indicated notable differences in moisture content, total soluble solids, titratable acidity, and carotenoid levels, with some traditional cultivars demonstrating high nutritional potential. Texture analysis showed variations in peel hardness, flesh firmness, and toughness, influencing storage capacity and consumer preference. Organoleptic evaluations highlighted the superior sensory attributes of cultivars such as ‘Golden Orange’, ‘Jonathan’, ‘Kaltherer Böhmer’, and ‘Golden Delicious’, which ranked highest in terms of taste, aroma, and juiciness. Statistical analyses, including principal component and hierarchical clustering analyses, further distinguished cultivars based on their physicochemical and sensory profiles. The findings emphasize the importance of genetic diversity in apples in maintaining a resilient and sustainable assortment. This study provides valuable insights for breeding programs and for orchard, market, and apple industry development. We also highlight future directions, promoting the conservation and strategic use of both traditional and modern cultivars. Full article

Food preservation techniques changed during the industrial revolution, as safer techniques were developed and democratized. However, one of the simplest techniques, adding salt, is still employed in a wide variety of products, not only as a flavor enhancer but as a method to allow for the controlled fermentation of products such as fruits. The objective of the present study consists of evaluating the quality of different salt-reduced fermented fruits through the application of the lactic acid bacteria (LAB) Lacticaseibacillus paracasei and vacuum, as well as assessing the LAB as a preventive measure against Escherichia coli O157:H7. To achieve this goal, microbial plate count techniques, the evaluation of the physicochemical characteristics, and Check-All-That-Apply/Rate-All-That-Apply sensory analyses were performed on bananas and apples individually fermented at 30 °C for 2 and 7 days, respectively. Additionally, a challenge test using E. coli as pathogenic bacteria was performed. The characteristics of each fruit determined the efficiency of the LAB’s protective activity. LAB-inoculated batches controlled the growth of E. coli in both salted fruits, but this pathogenic bacterium in the apple case was controlled even in the salt-reduced batch. Sensorially, only inoculated fermented apples found a reduction in off-flavor and old fruit smell; however, higher acceptability was found in the salt-reduced with LAB batches of both fruits. Thus, Lacticaseibacillus paracasei proved to be a cheap, easy, and feasible protective method that can ensure a protective strategy on salt-reduced fermented apples and should be studied particularly for different fruits. Full article

Flavor is the most important feature consumers use to examine fruit ripeness, and it also has an important influence on taste sensation. Nowadays, more and more consumers pay much attention not only to the appearance but also to the fruit’s aroma. Exploiting the potential of headspace solid-phase microextraction (HS-SPME) combined with sensitive two-dimensional gas chromatography and the time-of-flight mass spectrometry (GC/GC-ToF-MS) method within 30 old/traditional cultivars of apples (Malus domestica Borkh) coming from the same germplasm and 7 modern/commercial cultivars, 119 volatile organic compounds (VOCs) were identified. The largest group was esters (53), followed by alcohols (20), aldehydes (17), ketones (10), and acids (10). The richest volatile profile was ‘Grochówka’, with 61 VOCs present. The results revealed a visible difference based on VOC levels and profiles between the different apple cultivars, as well as visible similarities within the same cultivar coming from different farms. Based on a PCA, the commercial cultivars were separated into 7 clusters, including (1) ‘Gala’, (2) ‘Melrose’, (3) ‘Red Prince’, (4) ‘Lobo’, (5) ‘Ligol’, and (6) ‘Szampion’. The results of this study indicate that the profile of volatile compounds may be a useful tool for distinguishing between commercial and old apple cultivars, as well as for the varietal classification of apples from different locations. The developed method can also be used to identify other fruit varieties and origins based on their VOC composition. This may prove to be particularly valuable in the case of establishing a Protected Designation of Origin or Protected Geographical Indication. Full article

Apples are among the most consumed fruits worldwide. They serve as an excellent source of compounds that have a positive impact on human health. While new varieties of apples are being developed, numerous varieties have been forgotten. In this article, we present the results of research on 30 old apple cultivars, focusing on both qualitative and quantitative determination of antioxidant properties, and content of total phenolics, phenolic acids, triterpenoids and polyphenols. Our analyses show significant differences in the total content of each group of compounds between apple cultivars, as well as the phytochemical profile. The richest source of antioxidants was revealed to be ‘Reneta Blenheimska’ and ‘Książę Albrecht Pruski’ varieties, but the highest amount of phenolics had ‘James Grieve’ and ‘Kantówka Gdańska’ (KG). Among studied apples KG, ‘Krótkonóżka Królewska’ and ‘Grochówka’ (G) were the richest source of phenolic acids and polyphenols, whereas G, ‘James Grieve’ and ‘Krótkonóżka Królewska’ had the highest level of triterpenoids. Based on these findings, we selected two cultivars, G and KG, for further in vitro cell line-based studies. Based on biological activity analyses, we demonstrated not only antioxidant potential but also proapoptotic and cytoprotective properties within human-originated Caco-2 and HepG2 cell lines. In the era of a dynamically growing number of lifestyle diseases, it is particularly important to draw the attention of producers and consumers to the need to choose fruit varieties with the highest possible content of health-promoting compounds and, therefore, with the strongest health-promoting properties. Full article

This research is focused on comparing the compositions of juice produced from old and commercially grown apple cultivars. We examined factors such as pH, total acids, soluble dry matter, polyphenol profile, and antioxidant activity, which impact the attributes, safety, shelf life, and nutritional value of the juice. Our analysis revealed differences between these two groups of cultivars. For instance, pH values ranged from 3.04 (in ‘Bobovec’) to 3.69 (in ‘Fuji’). The proportions of acids varied from 0.07 g/100 mL (in ‘Fuji’) to 0.19 g/100 mL (in ‘Wagener’). Soluble dry matter content ranged from 14.10% (in ‘Fuji’) to 18.50% (in ‘Kraljevčica’). We also observed variations in sugar content and composition among cultivars; for example, sucrose levels varied from 16.11 g/L (‘Fuji’) to 39.36 g/L (‘Golden Delicious). Glucose levels ranged from 4.95 g/L (‘Jonagold’) to 19.18 g/L (‘Fuji’), while fructose levels spanned from 50.78 g/L (‘Austrougarka’) to 427.97 g/L (‘Ilzer Rosenapfel’). Furthermore, old apple cultivars exhibited higher concentrations of phenols and flavonoids compared to commercial ones; we also noted significant variations in flavonol levels among different cultivars. The ‘Wagener’ and ‘Božićnica’ apple varieties had levels of myricetin measuring 0.53 and 0.52 µg/mL, respectively. On the other hand, ‘Bobovec’ stood out for its content of procyanidin B2 with a concentration of 422.61 µg/mL. When examining non-flavonoid compounds, it was found that old apple cultivars had higher concentrations of gallic acid, trans-ferulic acid, and chlorogenic acid. However, commercial cultivars showed dominance in caffeic and p-coumaric. Comparisons of antioxidant capacity using DPPH and ABTS assays clearly demonstrated the superiority of old apple cultivars. Overall, this study highlights the importance of utilizing apple cultivars for juice production. Their distinct compositions and higher antioxidant capacities contribute to potential health benefits. Preserving these cultivars for enhanced juice quality and nutritional value is encouraged. Further research could explore cultivation practices’ impact on composition and health benefits. Full article

The aim of this study was to determine the quality characteristics and bioactive components of the local variety “Long Apple”. Although it is a very delicious and popular old apple variety, the knowledge about “Long Apple” is very insufficient. In this study, fruit quality parameters, organic acids, vitamin C, sugar components, phenolic compounds, and antioxidant capacity of the endemic apple “Long Apple” were determined as follows: chlorogenic acid, catechin, syringic acid, and o—coumaric acids, 117.68, 35.11, 22.71, and 15.54 mg kg⁻¹fw, respectively; vitamin c 135.67 mg L⁻¹, total sugar 196.29 g L⁻¹; malic, succinic, and citric acid 10.50, 2.88, and 2.13 g 100g⁻¹, respectively; fruit weight 139.11 g, hardness 8.27 kg cm⁻², shape index 1.42, and soluble solid content 16.70%; antioxidant capacity 3.30 µmol g⁻¹ TE were the highest. Except for fruit size and shape, the “Long Apple” genotypes outperformed Starking Delicious based on quality parameters and bioactive properties. The genotypes of “Long Apple” are separated by different parameters, but the genotypes 36K093, 36K094, and 36K106 are more prominent than others. In addition to its health benefits, the “Long Apple” has valuable properties for researchers. Therefore, increased research on “Long Apple” has been recommended. Full article

Low temperature is an abiotic stress factor limiting the distribution of fruit tree cultivation areas. As temperate deciduous fruit trees, apple (Malus domestica) trees go dormant in the winter to adapt to or avoid damage caused by low temperatures. The capacity for cold resistance is closely linked to the physiological, biochemical, and structural characteristics of one-year-old branches. In this study, we investigated such changes in the branches of cold-resistant ‘Hanfu’ (HF) and cold-sensitive ‘Naganofuji 2’ (CF) apple varieties. The relative electrical conductivity, malondialdehyde content, and reactive oxygen species content of HF branches were lower than those of CF branches, while the antioxidant enzyme activity was higher in HF. The proline, soluble protein, and soluble sugar contents in both varieties showed an initial increase, followed by a subsequent decrease. Sucrose and sorbitol were the main sugar components, but sucrose and fructose were higher in HF than in CF. The periderm, phloem, and xylem of HF branches were also found to be thicker than those of CF branches, while the vessel diameter was smaller and the density greater. The results of this study provide a theoretical reference for further research on the low temperature adaptability of apple tree branches during dormancy. Full article

Urban horticulture has to respond to several challenges, including reducing the growth vigor of the scion/rootstock combination, adapting the cultivated species/variety to the pedoclimatic conditions—more varied in recent years, and increasing tolerance to diseases and pests that cause extensive qualitative and quantitative damage to fruit production. For this experiment, 15 old apple varieties were chosen, all known and cultivated in the Banat area, which were grafted on the MM106 rootstock. Several parameters were analyzed that can be used for a relevant classification for planting them in limited spaces, such as in a private garden. Our research started 10 years ago and has already resulted in a doctoral thesis that extensively presents the data taken from the initial biological material and the first two years after grafting the trees; the research continued and is still ongoing in the experimental orchard. From the multitude of aspects studied, we have chosen to present some results related to the vigor of the scion/rootstock combination, the quality of the fruits, their content in acidity as well as the behavior with respect to diseases and pests—results that can be useful to those who want to grow old apple varieties in their own garden. Growth vigor was reduced in most varieties, but the highest influence of the rootstock on growth was observed in the ‘Bănățenesc’ variety, followed by ‘Jonathan de Munte’ and ‘Caslere’. Grafting and agrotechniques had a positive influence on the fruit mass in all cultivars, except ‘Crețesc’, with an experimental average of 163.35 g, namely 60.56 g higher on average than that of the original biological material. After grafting, the SSC was between 10.05 °Brix in the ‘Curcubătoase’ variety and 18.48 °Brix in ‘Parmen Auriu’, with an experimental average of 14.96 °Brix. The fruit acidity oscillated between 0.13 g/100 g in ‘Florănești’ and ‘Pătul’ varieties and 0.46 g/100 g in ‘Domnesc’, with an experimental average of 0.25 g/100 g. Full article

The bagging of fruits provides efficient protection from high-intensity sunlight and improves fruit color and quality. However, bagged fruit suddenly exposed to bright light can cause sunburn and destroys the peel cell structure. In this study, fruits from ten-year-old apple trees of ‘Gala’ variety were debagged, and the effect of sunburn on fruits was divided into: (1) normal peels (BFN), (2) peels with albefaction (BFA), and (3) browning (BFB). The non-bagging fruits (NBF) were set as a control to study the physiological characteristics of apple fruits with different levels of sunburn. Our results showed that in the early stages of debagged fruits’ sunburn, the cell structure of the peel was partially destroyed, the color of the injured fruit surface turned white, and the peroxidation in the cell membrane of the peel increased. Initially, the fruit improved its photosynthetic protection ability, and the activity of antioxidants and phenolics was enhanced, to cope with external injury. However, with the increase in duration of high-intensity sunlight, the cell structure of the peel was severely damaged, and the increase in membrane peroxidation resulted in brown coloration of fruits. Under the same conditions, the photoprotection ability and antioxidant enzyme activity of non-bagged fruits showed higher levels. In conclusion, the non-bagged fruits were more adaptable to high-intensity sunlight as compared to debagged fruits. Full article

(1) Background: The aim of this research is to study the importance of improving knowledge of old variety apples in terms of phytochemicals, antioxidant capacity and mineral elements. (2) Methods: Fifteen phenolic compounds (UHPLC-MS/MS techniques), eight mineral elements (Ca, K, Cu, Mn, Fe, Zn, Na and Mg), antioxidant capacity (DPPH%) and vitamin C content in four ancient apple varieties of Tuscany (‘Mora’, ‘Nesta’, ‘Panaia’ and ‘Ruggina’), with the ‘Golden Delicious’ cultivar as reference, were analyzed. (3) Results: Ancient cultivars exhibited a superior antioxidant capacity compared to commercial cultivars. ECTC and CGA were the polyphenols that mostly characterized the ancient apples. The contents of the elements of concern, Zn and Mn, were higher in the ancient cultivars ‘Panaia’ and ‘Ruggina’, while Na showed significant low concentration in ‘Nesta’, ‘Panaia’ and ‘Ruggina’ compared to ‘Golden Delicious’. The vitamin C content also indicated that ‘Mora’, ‘Panaia’ and ‘Ruggina’ old variety had an AsA content around 10 times higher than the ‘Golden Delicious’ apple. (4) Conclusions: Underutilized varieties could be an excellent source of bioactive phenolic phytochemicals, mineral nutrients and vitamins that may offer special nutraceutical benefits compared to other fruits. Full article

Managing irrigation efficiently is paramount given the uncertainty in the future availability of water and rising demand for this resource. Scheduled irrigation significantly influences vegetative growth through improving crop physiology and nutrient uptake and use efficiency. Influence of different irrigation treatments (100%, 75%, and 50% volume of Class A pan evapotranspiration) applied at four different phenological stages (flowering and fruit set (C1), fruit growth stage (C2), pre-harvest stage (C3), and throughout the growing season (C4)) through drip along with a control (rainfed) on leaf physiology, nutrient content, and uptake through gene expression was studied on Super Chief Sandidge variety raised on M9T337 (5 and 6 years old) grown at a spacing of 1.5 × 3 m (2222 plants/ha) under high density condition of Kashmir Himalayan range of India. A comparison of data reveals that drip irrigation at 100% Crop evapotranspiration (ETc) increased leaf area by 60% compared to rainfed conditions. Leaf area significantly increased in plants irrigated throughout the growing season (C4) and during flowering and fruit set stage (C1). Irrigation amount likely does not have any influence on leaf development after the fruit growth stage. Stomatal opening and their size greatly vary from no irrigation to optimum irrigation in these plants. High density apple trees exposed to optimum irrigation levels (100% and 75% ET) had significantly higher concentrations of nutrients (N, P, and K) in their leaf tissues. The concentration of Ca and Mg content in leaf tissues are greatly influenced by the optimum supply of water during the early growth stages of apple growth. The availability of water significantly influences nutrient transporter gene expression and thus nutrient uptake by regulating such transporter genes. It is therefore observed that proper irrigation during C1 and stage C2 stage are the critical growth stages of apple for optimum leaf physiological activity and proper nutrient uptake. Full article

In this study, a metabolomic investigation was presented to correlate single polyphenolic compounds in apple pulp with quality characteristics such as antioxidant activity and content of phenolic compounds and anthocyanins in apple skin. Since the concentration of these compounds is influenced by environmental factors, the twenty-two apple cultivars originate from the same site. The polyphenolic compounds were analyzed by ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). The antioxidant activity, phenolic content, and anthocyanins were evaluated on the sunny and the shady sides of apple skin by spectrometric assays. In old apple varieties, the measured parameters were higher than in the commercial and red-fleshed varieties. By contrast, the profile of flavan-3-ols and anthocyanins was variable amongst commercial and red-fleshed varieties. The partial least square (PLS) method was applied to investigate the association between the skin proprieties and the metabolic profile of the pulp. The highest coefficients of determination in prediction (Q2) were obtained for compounds quantified in old cultivars. These results provided information to define the old apple varieties as a reliable group based on the pathway of the antioxidant compounds and anthocyanins content. Our results show the possibility to find cultivars with promising health features based on their content of polyphenols suitable for commercialization or breeding. Full article

About 50–70% of patients allergic to birch pollen suffer from sensitization after apple ingestion. Apple allergenicity was established in only few varieties. Studies were performed on apple fruits of 21 traditional and nine modern varieties organically, intensively, or integratively produced. The aim of the study was to assess whether the factors like cultivation method, maturity stage, genotype, or type of tissue place an impact on the allergenic potential of apples. To answer these questions, we used semiquantitative real-time PCR, ELISA, and immunoblotting. Apple allergen genes present divergent expression across apple cultivars. Expression of the Mal d 1.06A correlates with the Mal d 1 level and is affected by the cultivation method and maturity of the fruit. The content of the main allergen Mal d 1 varied widely across cultivars. Interestingly, in our study, the Gala variety presented a low Mal d 1 concentration regardless of the cultivation method. Based on the Mal d 1.06A expression, the Mal d 1 protein content, and the immunoreactivity assay, the Kandil Sinap, Kosztela, Rumianka from Alma-Ata, Kantówka Gdańska, Reinette Coulon, and Gala cultivars emerged as potentially hypoallergenic apple cultivars. Our study allowed distinguishing between potentially low, medium, and highly allergenic varieties. Full article