Search Results (16)

Medicinal plants have attracted increasing scientific interest due to the diversity of bioactive compounds reported across different species. They may represent complementary sources of bioactive compounds alongside conventional antimicrobials, which may pose risks to animal health and compromise treatment efficacy. Considering the importance of alternative compounds, we aimed to evaluate the antimicrobial activity in vitro of medicinal plants Stryphnodendron adstringens (Mart.) Coville, known as barbatimão, Baccharis crispa Spreng, known as carqueja and Azadirachta indica, known as neem. S. adstringens (Mart.) Coville and B. crispa Spreng were used as extract and obtained from plants collected in the municipality of Bambuí, state of Minas Gerais, Brazil. A. indica was evaluated as extract and oil, and the crushed leaves and oil were purchased from a commercial company. Antimicrobial activity was determined by the minimum bactericidal concentration (MBC) test-against Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Escherichia coli, and Salmonella spp., isolated from bovine mastitis. The bacteria were submitted to the MBC test at concentrations of 100, 50, 25, 12.5, 6.25, 3.12, 1.56, 0.78, 0.39, 0.19 and 0.09 mg/mL. The bacteria evaluated were sensitive to most plant extracts for at least one of the concentrations evaluated, except for Gram-negative bacteria, Escherichia coli, and Salmonella spp. There was no activity of B. crispa Spreng extract and A. indica against E. coli and neither of B. crispa Spreng extract against Salmonella spp. even at the highest concentration evaluated. S. adstringens (Mart.) Coville was considered the extract with the highest activity against the bacteria evaluated and S. uberis the most susceptible to antimicrobial action. The results indicate detectable antimicrobial activity of the evaluated extracts and oil, suggesting their potential relevance as complementary sources of bioactive compounds for further investigation, rather than as direct alternatives to conventional antibiotic therapies. Paper spray mass spectrometry (PS-MS) was employed as an exploratory phytochemical screening approach, and all metabolite assignments reported herein should be regarded as tentative or putative annotations under the analytical conditions used, consistent with MSI Level 3 confidence. Full article

Mating and feeding behavioural differences have been observed between male and female wasps (Hymenoptera: Aculeata). It is hypothesised that these behavioural differences are supported by morphological dimorphisms in their sensory systems. Here, this hypothesis was tested in the parasitoid aculeate wasp Scolia hirta (Scoliidae), which attacks beetle larvae located in the soil. We have found evidence of a link between the main stimuli used by the sexes to access their target resources and the morphology of the sensory system. Males have a more developed visual system with enlarged eyes, ocelli and a higher number of ommatidia, sinc they have to visually locate and chase females immediately after they emerge. Males possess nine types of sensilla (primarily hygrothermo- and mechanoreceptors), and females possess seven types of sensilla, primarily olfactory. In females, the sensilla placoidea occur in greater numbers and are larger in size, in line with their underground host-seeking behaviour. Furthermore, the females had a blunt tip on their distal flagellomere and wider fore tibiae, which suggests that they may use vibrational sounding to detect concealed hosts, similarly to what has been described for some non-aculeate parasitoid wasps. Full article

Bristletails (Microcoryphia) represent a relatively understudied group of class Insecta. These wingless hexapods, which retain numerous plesiomorphic structures, remain less well understood compared to most other insect lineages. Through detailed morphological examination of specimens collected from southern China, we describe six new species (Pedetontus (Verhoeffilis) elegans sp. n. from Zhejiang; P. (V.) hezhouensis sp. n., P. (V.) jinxiuensis sp. n., and P. (V.) nanningensis sp. n. from Guangxi; and P. (V.) shenzhenensis sp. n. and P. (V.) xanthospilus sp. n. from Guangdong) of subgenus Verhoeffilis Paclt, 1972. This subgenus is distinguished from other members of the Petrobiinae by several diagnostic features: paired ocelli shoe-shaped (fusiform-shaped) and submedian; two pairs of retractile vesicles on abdominal segments II–V; parameres restricted to coxite IX; penis opening small and apical; male genitalia not exceeding coxite IX; and ovipositor of primary type. The new species can be distinguished from known species and each other by the morphology of the compound eyes, maxilla, labial palps, legs, and genitalia; here, we provide detailed illustrations, tables, and descriptions. Furthermore, we sequence the COX1 gene to construct a phylogenetic tree of Microcoryphia. This contribution increases the total number of reported Pedetontus species in China to seventeen. Full article

The quarantine pest, Cylas formicarius, is a key pest of sweet potatoes during both production and storage, posing a major threat to food security in various countries. To investigate behavioral mechanisms, the ultrastructure of the heads of larval and adult stages was analyzed using scanning electron microscopy, with an emphasis on the sensilla of the mouthparts and antennae. The results reveal degeneration of the antennae and ocelli in larvae. The larval mouthparts are equipped with three types and six subtypes of sensilla. Both male and female adults have four types and six subtypes of sensilla on their mouthparts. Compared to larvae, the adult mouthparts display a greater diversity of sensilla types and higher numbers of sensilla basicaonica (SB), sensilla chaetica (SC), and sensilla digitiformia (SD). Adult antennae consist of a scape, a pedicel, and eight flagellomeres (F1–F8), with F8 showing sexual dimorphism. Seven types of sensilla, excluding SB and sensilla ligulate (SL), each with two subtypes, were identified on the antennae of adults of both sexes. SC, sensilla furcatea, Böhm bristles, and SL were newly observed in the antennae of C. formicarius adults. Additionally, one type and seven subtypes of sensilla on the adult antennae exhibit distinct sexual dimorphism in terms of structure or number. The relationship between the head structure and adaptability of C. formicarius was examined, and the functions of each sensilla were discussed, providing a theoretical basis for future studies on the behavior of this pest. Full article

The pine leafhopper genus Pinopona Viraktamath & Sohi was previously classified within the tribe Grypotini. In a recognized classification system, it has been categorized as the subtribe Grypotina of the tribe Koebeliini within Deltocephalinae, based on morphological characteristics such as a crown exhibiting few transverse fine striations along the anterior margin; short antennae measuring less than 1.5 times the head length but not extending to half or more of body length; ocelli positioned below the anterior margin of the crown and not visible from above, with a considerable distance from the eyes; and an anteclypeus that is narrow and tapered beyond the anterior margin of gena, alongside metatarsomere I presenting platellae on the plantar surface. Two new species, Pinopona gongshanensis and Pinopona daliensis spp. nov., are described herein from Yunnan Province and illustrated accordingly. This study also provides a checklist and key for all species within this genus. Full article

One new genus (Ocrognethoda gen. nov.) and three new species of webspinners (Ocrognethoda olivea gen. et sp. nov., Gnethoda lata sp. nov. and Parasorellembia hamata sp. nov.) are described from the Upper Cretaceous of Myanmar amber. Ocrognethoda olivea gen. et sp. nov. and Gnethoda lata sp. nov. are attributed to the family Clothodidae due to their simplified and symmetrical male terminalia, in which the tenth tergum is undivided. Parasorellembia hamata sp. nov. is classified in the family Scelembiidae by a specialized abdominal apex: fused cerci, a broad right hemitergite of the tenth abdominal tergite, and ocelli presence. Moreover, based on the discovery of new genus and species, the male terminalia of Clothodidae and Sorellembiinae are briefly discussed. Full article

Sapindales is a large order with a great diversity of nectaries; however, to date, there is no information about extrafloral nectaries (EFN) in Sapindaceae, except recent topological and morphological data, which indicate an unexpected structural novelty for the family. Therefore, the goal of this study was to describe the EFN in Sapindaceae for the first time and to investigate its structure and nectar composition. Shoots and young leaves of Urvillea ulmacea were fixed for structural analyses of the nectaries using light and scanning electron microscopy. For nectar composition investigation, GC-MS and HPLC were used, in addition to histochemical tests. Nectaries of Urvillea are circular and sunken, corresponding to ocelli. They are composed of a multiple-secretory epidermis located on a layer of transfer cells, vascularized by phloem and xylem. Nectar is composed of sucrose, fructose, xylitol and glucose, in addition to amino acids, lipids and phenolic compounds. Many ants were observed gathering nectar from young leaves. These EFNs have an unprecedented structure in the family and also differ from the floral nectaries of Sapindaceae, which are composed of secretory parenchyma and release nectar through stomata. The ants observed seem to protect the plant against herbivores, and in this way, the nectar increases the defence of vegetative organs synergistically with latex. Full article

Circadian entrainment to the environmental day–night cycle is essential for the optimal use of environmental resources. In insects, opsin-based photoreception in the compound eye and ocelli and CRYPTOCHROME1 (CRY1) in circadian clock neurons are thought to be involved in sensing photic information, but the genetic regulation of circadian light entrainment in species without light-sensitive CRY1 remains unclear. To elucidate a possible CRY1-independent light transduction cascade, we analyzed light-induced gene expression through RNA-sequencing in Nasonia vitripennis. Entrained wasps were subjected to a light pulse in the subjective night to reset the circadian clock, and light-induced changes in gene expression were characterized at four different time points in wasp heads. We used co-expression, functional annotation, and transcription factor binding motif analyses to gain insight into the molecular pathways in response to acute light stimulus and to form hypotheses about the circadian light-resetting pathway. Maximal gene induction was found after 2 h of light stimulation (1432 genes), and this included the opsin gene opblue and the core clock genes cry2 and npas2. Pathway and cluster analyses revealed light activation of glutamatergic and GABA-ergic neurotransmission, including CREB and AP-1 transcription pathway signaling. This suggests that circadian photic entrainment in Nasonia may require pathways that are similar to those in mammals. We propose a model for hymenopteran circadian light-resetting that involves opsin-based photoreception, glutamatergic neurotransmission, and gene induction of cry2 and npas2 to reset the circadian clock. Full article

A new species, Belbolla mangrove sp. nov., isolated from the mangrove wetlands of Fujian and Zhejiang Provinces in China, is described and illustrated. Belbolla mangrove sp. nov. is characterized by a pharynx with four bulbs, a small gubernaculum with a short dorsocaudal apophysis, four weakly developed precloacal supplements, a conico-cylindrical tail with a terminal spinneret, and the absence of terminal setae. This new species differs from B. vietnamica by the absence of ocelli and the blunt and rounded proximal ends of the spicules. The 18S rDNA GenBank accession numbers of B. mangrove sp. nov. are provided. Full article

The continuous processes of mining development, since the very beginning of Minas Gerais State’s development, have been giving new attention and meaning to valuable pre-existing features (i.e., cultural, social, and physical-environmental), impacting and recharacterizing not only its municipalities but their essential local or native sociocultural components. At the same time, mining, as one of the central pillars of the Brazilian development model, has put different communities, natural and cultural heritage, and mineral and water resources at risk. The wide concept of geodiversity and the related geoheritage emerge as an alternative for conservation, territorial planning, and sustainable development, to reconcile these spheres. This study developed a comprehensive framework for geoconservation within selected areas of mining landscapes, contributing to insights for the creation of a catalog about geoheritage in the state of Minas Gerais, discussing and analyzing well-established strategies and opportunities based on UNESCO Global Geoparks (UGGp) and the GEOfood brand. We concluded that the mining landscapes of Minas Gerais must be administered as a viable possibility for economic and environmental dynamic actions and activities, strengthening the maintenance of municipalities from the very beginning to after the end of operational activities. Heritage programs such as UGGp and GEOfood enable knowledge sharing and engagement with geoheritage, improving the comprehension and management of the short- and long-term impacts of mining, while elevating geodiversity as a major source of information in the “greening” of mining policies. Full article

Vision is essential for most organisms, and it is highly variable across kingdoms and domains of life. The most known and understood form is animal and human vision based on eyes. Besides the wide diversity of animal eyes, some animals such as cuttlefish and cephalopods enjoy so-called dermal or skin vision. The most simple and ancient organ of vision is the cell itself and this rudimentary vision evolved in cyanobacteria. More complex are so-called ocelloids of dinoflagellates which are composed of endocellular organelles, acting as lens- and cornea/retina-like components. Although plants have almost never been included into the recent discussions on organismal vision, their plant-specific ocelli had already been proposed by Gottlieb Haberlandt already in 1905. Here, we discuss plant ocelli and their roles in plant-specific vision, both in the shoots and roots of plants. In contrast to leaf epidermis ocelli, which are distributed throughout leaf surface, the root apex ocelli are located at the root apex transition zone and serve the light-guided root navigation. We propose that the plant ocelli evolved from the algal ocelloids, are part of complex plant sensory systems and guide cognition-based plant behavior. Full article

Convolutional Neural Networks (CNNs), due to their recent successes, have gained lots of attention in various vision-based applications. They have proven to produce incredible results, especially on big data, that require high processing demands. However, CNN processing demands have limited their usage in embedded edge devices with constrained energy budgets and hardware. This paper proposes an efficient new architecture, namely Ocelli includes a ternary compute pixel (TCP) consisting of a CMOS-based pixel and a compute add-on. The proposed Ocelli architecture offers several features; (I) Because of the compute add-on, TCPs can produce ternary values (i.e., −1, 0, +1) regarding the light intensity as pixels’ inputs; (II) Ocelli realizes analog convolutions enabling low-precision ternary weight neural networks. Since the first layer’s convolution operations are the performance bottleneck of accelerators, Ocelli mitigates the overhead of analog buffers and analog-to-digital converters. Moreover, our design supports a zero-skipping scheme to further power reduction; (III) Ocelli exploits non-volatile magnetic RAMs to store CNN’s weights, which remarkably reduces the static power consumption; and finally, (IV) Ocelli has two modes, including sensing and processing. Once the object is detected, the architecture switches to the typical sensing mode to capture the image. Compared to the conventional pixels, it achieves an average 10% efficiency on its lane detection power consumption compared with existing edge detection algorithms. Moreover, considering different CNN workloads, our design shows more than 23% power efficiency over conventional designs, while it can achieve better accuracy. Full article

Agricultural systems comprise an interdisciplinary field that studies the complex dimensions of agriculture. They should not be characterized only by their agricultural value, as they are part of several social, cultural, geological, and historical domains. We carried out quantitative and qualitative research to present and compare the current state of agricultural heritage programs and their development in Brazil and Italy, contrasting with the Globally Important Agricultural Heritage Systems (GIAHS) by the Food and Agriculture Organization (FAO). To this end, the history and the extension of these programs and sites were recovered. Moreover, the agricultural landscape diversity, the development of the regions, research and outreach, along with the communities, entities and government bodies involved were identified. Through a combination and quality of technical assessment and communities’ description, the analyzed agricultural heritage programs prove to be an endless source of useful information to the definition of policies aimed at rural areas, in addition to serving as a monitoring tool for many issues regarding biocultural diversity in landscape. Moreover, it shows where there is room for improvement while the countries are committed to engaging in national policies and entities on the promotion of agricultural heritage programs as major steps for investing in the “greening” of agricultural policies at different levels. Full article

The study reports petrography, mineralogy and carbonate geochemistry and stable isotopy of various types of ocelli (silicate-carbonate globules) observed in the lamprophyres from the Chadobets Uplift, southwestern Siberian craton. The Chadobets lamprophyres are related to the REE-bearing Chuktukon carbonatites. On the basis of their morphology, mineralogy and relation with the surrounding groundmass, we distinguish three types of ocelli: carbonate-silicate, containing carbonate, scapolite, sodalite, potassium feldspar, albite, apatite and minor quartz ocelli (K-Na-CSO); carbonate–silicate ocelli, containing natrolite and sodalite (Na-CSO); and silicate-carbonate, containing potassium feldspar and phlogopite (K-SCO). The K-Na-CSO present in the most evolved damtjernite with irregular and polygonal patches was distributed within the groundmass; the patches consist of minerals identical to minerals in ocelli. Carbonate in the K-Na-CSO are calcite, Fe-dolomite and ankerite with high Sr concentration and igneous-type REE patterns. The Na-CSO present in Na-rich damtjernite with geochemical signature indicates the loss of the carbonate component. Carbonate phases are calcite and Fe-dolomite, and they depleted in LREE. The K-SCO was present in the K-rich least-evolved damtjernite. Calcite in the K-SCO has the highest Ba and the lowest Sr concentration and U-shaped REE pattern. The textural, mineralogical and geochemical features of the ocelli and their host rock can be interpreted as follows: (i) the K-Na-CSO are droplets of an alkali–carbonate melt that separated from residual alkali and carbonate-rich melt in highly evolved damtjernite; (ii) the Na-CSO are droplets of late magmatic fluid that once exsolved from a melt and then began to dissolve; (iii) the K-SCO are bubbles of K-P-CO₂ fluid liberated from an almost-crystallised magma during the magmatic–hydrothermal stage. The geochemical signature of the K-SCO carbonate shows that the late fluid could leach REE from the host lamprophyre and provide for REE mobility. Full article

To evaluate the effect of Bt maize expressing Cry1Ie protein on non-target soil Collembola, a two-year field study was conducted in Northeast China. Bt maize line IE09S034 and its near isoline Zong 31 were selected as experimental crops; we investigated the collembolan community using both taxonomic and trait-based approaches, and elucidated the relationship between environmental variables and the collembolan community using redundancy analysis (RDA).The ANOVA results showed that maize variety neither had significant effect on the parameters based on taxonomic approach (abundance, species richness, Shannon–Wiener index, Pielou’s evenness index), nor on the parameters based on trait-based approach (ocelli number, body length, pigmentation level, and furcula development) in either year. The results of RDA also showed that maize variety did not affect collembolan community significantly. These results suggest that two years cultivation of cry1Ie maize does not affect collembolan community in Northeast China. Full article