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Keywords = occupational electromagnetic exposure

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26 pages, 6104 KB  
Article
Electromagnetic Exposure from RF Antennas on Subway Station Attendant: A Thermal Analysis
by Jin Li, Qianqian Zhang and Mai Lu
Sensors 2026, 26(2), 709; https://doi.org/10.3390/s26020709 - 21 Jan 2026
Abstract
With the rapid development of wireless communication systems, the electromagnetic environment in subway stations has become increasingly complex, raising concerns about the long-term safety of station attendants who are chronically exposed to radiofrequency (RF) fields. At present, multiphysics analyses specifically addressing RF antenna [...] Read more.
With the rapid development of wireless communication systems, the electromagnetic environment in subway stations has become increasingly complex, raising concerns about the long-term safety of station attendants who are chronically exposed to radiofrequency (RF) fields. At present, multiphysics analyses specifically addressing RF antenna exposure scenarios for subway attendants remain limited. To assess occupational electromagnetic exposure risks, this paper establishes a comprehensive electromagnetic–thermal coupling simulation model incorporating RF antennas, station-platform structures, and a realistic human model with organs including the brain, heart, and liver. Using the finite-element software COMSOL Multiphysics (v.6.3), numerical simulations are performed to calculate the specific absorption rate (SAR) in the trunk and major organs of the subway station attendant at RF antennas frequencies of 900 MHz, 2600 MHz, and 3500 MHz, as well as the temperature rise distribution of the human trunk and important tissues and organs under different initial temperatures of the environment. The results show that among the three frequencies, the maximum SAR of 5.55 × 104 W/kg occurs in the trunk at 3500 MHz. Tissue temperatures reach thermal steady state after 30 min of exposure, with the maximum temperature rises occurring in the brain at an ambient temperature of 18 °C and an operating frequency of 900 MHz, reaching 0.2123 °C. Across all simulated scenarios, both SAR values and temperature rises remain significantly below the occupational exposure limits established by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). These findings indicate that RF radiation generated by antennas in the subway station environment poses low health risks to female station attendants of similar physical characteristics to the Ella model. This study provides a scientific reference for the occupational RF protection of subway personnel and contributes data for the development of electromagnetic exposure standards in rail transit systems. Full article
(This article belongs to the Section Physical Sensors)
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22 pages, 699 KB  
Review
Causes of Childhood Cancer: A Literature Review (2014–2021)—Part 3: Environmental and Occupational Factors
by Rebecca T. Emeny, Mary E. Butow, Linda Titus, Angela M. Ricci, Pamela J. Bagley, Heather B. Blunt, Alexandra Morgan, Jennifer A. Alford-Teaster, Raymond R. Walston and Judy R. Rees
Cancers 2025, 17(21), 3516; https://doi.org/10.3390/cancers17213516 - 31 Oct 2025
Viewed by 1739
Abstract
Purpose: To achieve a better understanding of the environmental factors that contribute to childhood cancers, so as to inform future prevention efforts. Methods: We conducted a comprehensive review of epidemiological studies on environmental risk factors and childhood cancer, which was published between January [...] Read more.
Purpose: To achieve a better understanding of the environmental factors that contribute to childhood cancers, so as to inform future prevention efforts. Methods: We conducted a comprehensive review of epidemiological studies on environmental risk factors and childhood cancer, which was published between January 2014 and March 2021. Potential exposure sources presented in this review include air pollution, radiation, and parental occupational exposures. We considered exposures during childhood and parental exposures occurring before the child’s conception and during pregnancy in relation to all types of childhood cancer. Results: Aggregated evidence is strong for associations between leukemia and parental/child exposure to traffic pollution, indoor paints, residential pesticides, and parental occupational/nonoccupational exposure to benzene. Evidence is also strong for associations between brain cancer and residential pesticides and parental occupational exposure to agricultural pesticides. Evidence of associations between leukemia and ionizing radiation from radon and nuclear power plants remains mixed, as does evidence of a link between electromagnetic fields (EMFs) and childhood leukemia. Conclusions: Clear associations have been demonstrated between childhood cancer and environmental factors, including parent/child exposure to traffic pollution, occupational/nonoccupational benzene, indoor paints, residential pesticides, and parental occupational exposure to agricultural pesticides. These associations can be used to inform further study of interventions and public health campaigns to reduce risk. Full article
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23 pages, 14833 KB  
Article
Assessment of Electromagnetic Exposure Levels for Humans from Electric Vehicle DC Charging Stations
by Shaowen Dong and Mai Lu
Sensors 2025, 25(18), 5735; https://doi.org/10.3390/s25185735 - 14 Sep 2025
Viewed by 4774
Abstract
The potential health risks of DC charging piles to human health were investigated by quantifying the internal electromagnetic exposure level. In this study, the transformer in the DC/DC circuit of a DC charging pile was selected as the radiation source, and two realistic [...] Read more.
The potential health risks of DC charging piles to human health were investigated by quantifying the internal electromagnetic exposure level. In this study, the transformer in the DC/DC circuit of a DC charging pile was selected as the radiation source, and two realistic human models (adult and child) were used as exposure subjects. A simulation model, including the vehicle body, charging pile, and transformer, was established using COMSOL(COMSOL Multiphysics 6.2) Multiphysics software to calculate the magnetic induction intensity (B-field) and electric field intensity (E-field) in various organs at distances of 0.1 m, 0.3 m, and 0.6 m from the charging pile. The results show that at 0.1 m, the peak B-field (1.91 µT) and E-field (447 mV/m) in the adult body were 1.91% and 2.07% of the ICNIRP occupational exposure limits, respectively, and 7.07% and 4.14% of the public exposure limits. For the child model, the peak electromagnetic exposure levels (2.31 µT and 259 mV/m) were only 8.56% and 2.40% of the public limits. Further evaluation of exposure levels for in-vehicle occupants during charging showed that the peak B-field and E-field for an adult driver and a child in the front passenger seat were 0.0225 × 10−2 µT, 0.0237 × 10−2 µT, 5.81 mV/m, and 5.82 mV/m, respectively, far below the ICNIRP public limits. Additionally, analyses at multiple frequency bands (85 kHz, 90 kHz, and 95 kHz) under a typical scenario (adult at 0.1 m from the charging pile) revealed that the B-field in the human body decreased with increasing frequency, while the E-field showed minimal variation due to shielding effects. All electromagnetic exposure levels were below both ICNIRP public and occupational limits, indicating the broad applicability of the results. Under normal operating conditions of DC charging piles, the electromagnetic exposure from the DC/DC transformer fully complies with safety standards and poses no threat to human health. This study provides a scientific basis for alleviating public concerns about the health risks of electromagnetic radiation from DC charging piles for electric vehicles. Full article
(This article belongs to the Section Vehicular Sensing)
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23 pages, 7503 KB  
Article
EMF Exposure of Workers Due to 5G Private Networks in Smart Industries
by Peter Gajšek, Christos Apostolidis, David Plets, Theodoros Samaras and Blaž Valič
Electronics 2025, 14(13), 2662; https://doi.org/10.3390/electronics14132662 - 30 Jun 2025
Viewed by 1938
Abstract
5G private mobile networks are becoming a platform for ‘wire-free’ networking for professional applications in smart industry sectors, such as automated warehousing, logistics, autonomous vehicle deployments in campus environments, mining, material processing, and more. It is expected that most of these Machine-to-Machine (M2M) [...] Read more.
5G private mobile networks are becoming a platform for ‘wire-free’ networking for professional applications in smart industry sectors, such as automated warehousing, logistics, autonomous vehicle deployments in campus environments, mining, material processing, and more. It is expected that most of these Machine-to-Machine (M2M) and Industrial Internet of Things (IIoT) communication paths will be realized wirelessly, as the advantages of providing flexibility are obvious compared to hard-wired network installations. Unfortunately, the deployment of private 5G networks in smart industries has faced delays due to a combination of high costs, technical challenges, and uncertain returns on investment, which is reflected in troublesome access to fully operational private networks. To obtain insight into occupational exposure to radiofrequency electromagnetic fields (RF EMF) emitted by 5G private mobile networks, an analysis of RF EMF due to different types of 5G equipment was carried out on a real case scenario in the production and logistic (warehouse) industrial sector. A private standalone (SA) 5G network operating at 3.7 GHz in a real industrial environment was numerically modeled and compared with in situ RF EMF measurements. The results show that RF EMF exposure of the workers was far below the existing exposure limits due to the relatively low power (1 W) of indoor 5G base stations in private networks, and thus similar exposure scenarios could also be expected in other deployed 5G networks. In the analyzed RF EMF exposure scenarios, the radio transmitter—so-called ‘radio head’—installation heights were relatively low, and thus the obtained results represent the worst-case scenarios of the workers’ exposure that are to be expected due to private 5G networks in smart industries. Full article
(This article belongs to the Special Issue Innovations in Electromagnetic Field Measurements and Applications)
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10 pages, 2117 KB  
Article
Assessment of Interference in CIEDs Exposed to Magnetic Fields at Power Frequencies: Induced Voltage Analysis and Measurement
by Mengxi Zhou, Djilali Kourtiche, Julien Claudel, Patrice Roth, Isabelle Magne, François Deschamps and Bruno Salvi
Bioengineering 2025, 12(7), 677; https://doi.org/10.3390/bioengineering12070677 - 20 Jun 2025
Viewed by 821
Abstract
Despite ongoing concerns about electromagnetic interference affecting cardiac implantable electronic devices (CIEDs) in the electrical industry workplaces, no study has experimentally assessed induced voltages in CIEDs under exposure to power-frequency magnetic fields. This study addresses this gap by quantifying such interference using a [...] Read more.
Despite ongoing concerns about electromagnetic interference affecting cardiac implantable electronic devices (CIEDs) in the electrical industry workplaces, no study has experimentally assessed induced voltages in CIEDs under exposure to power-frequency magnetic fields. This study addresses this gap by quantifying such interference using a dedicated experimental setup to reproduce high intensity magnetic fields and to measure voltages induced on CIEDs under exposure. A thorough analysis was carried out in comparison with formula-based and simulation approaches applied in previous studies. The induced voltages on CIEDs were measured across varying configurations, including sensing mode, implantation method, exposure frequency, and magnetic field orientation. Our findings reveal the induced voltage levels under exposure from a statistical perspective and highlight correlations between susceptibility and the impact factors, with unipolar configurations and left pectoral implants exhibiting the highest susceptibility. This work provides insights into electromagnetic interference risks for CIED carriers and supports the development of individual protection strategies to enhance occupational safety. Full article
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17 pages, 3923 KB  
Article
The Parametrization of Electromagnetic Emissions and Hazards from a Wearable Device for Wireless Information Transfer with a 2.45 GHz ISM Band Antenna
by Patryk Zradziński, Jolanta Karpowicz and Krzysztof Gryz
Appl. Sci. 2025, 15(12), 6602; https://doi.org/10.3390/app15126602 - 12 Jun 2025
Viewed by 1034
Abstract
The parameters of electromagnetic emissions from the antenna of a wearable radio communication module (parameterizing device functionality) were investigated at different positions near the body where an antenna is located. The specific absorption rate (SAR) coefficient was also investigated as a way of [...] Read more.
The parameters of electromagnetic emissions from the antenna of a wearable radio communication module (parameterizing device functionality) were investigated at different positions near the body where an antenna is located. The specific absorption rate (SAR) coefficient was also investigated as a way of parameterizing the absorption of electromagnetic radiation in the user’s body adjacent to the antenna in various locations. The modeled exposure scenarios concerned a body-worn device with a 2.45 GHz ISM band antenna (used, e.g., for Wi-Fi 2G/Bluetooth applications). The antennas were modeled as follows: (1) located directly on the body (considered to be a model of a disposable, adhesive device) or (2) next to the body (considered to be a model of a classic, reusable, wearable electronic device located inside a plastic housing). Several body sections adjacent to the antenna were considered: head, arm, forearm, and chest (simplified and anatomical body models were used). The numerical models of the exposure scenarios were verified by relevant laboratory tests using physical models. It was found that the use of simplified models of the human body (numerical or physical) may be sufficient when analyzing antenna performance and SAR in a user’s body, such as in studies regarding microwave imaging and sensing, wireless implantable devices, wireless body-area networks or SAR estimation. Full article
(This article belongs to the Special Issue Antennas for Next-Generation Electromagnetic Applications)
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20 pages, 4838 KB  
Article
Assessment of RF Electromagnetic Exposure to Car Driver from Monopole Array Antennas in V2V Communications Considering Thermal Characteristics
by Shirun Wang and Mai Lu
Sensors 2025, 25(10), 3247; https://doi.org/10.3390/s25103247 - 21 May 2025
Viewed by 1358
Abstract
Vehicles are rapidly evolving into objects of intelligent interconnection. Vehicle-to-Vehicle (V2V) communications enable the interconnection between vehicles, while also leading to new electromagnetic exposure scenarios. This paper integrates a monopole array antenna into a shark-fin antenna on the car roof for V2V communications [...] Read more.
Vehicles are rapidly evolving into objects of intelligent interconnection. Vehicle-to-Vehicle (V2V) communications enable the interconnection between vehicles, while also leading to new electromagnetic exposure scenarios. This paper integrates a monopole array antenna into a shark-fin antenna on the car roof for V2V communications and evaluates the specific absorption rate (SAR) and temperature rise of a human body in a smart mobility communication scenario operating at 5.9 GHz. The V2V antenna is modeled and placed on a 3D vehicle model using COMSOL Multiphysics (v.6.2) to numerically estimate the SAR in the head and body regions of the human body model (adult male) inside the vehicle. Both the localized and whole-body 30 min average SAR are lower than the International Commission on Non-Ionizing Radiation Protection (ICNIRP) occupational restrictions for electromagnetic field exposure from 100 kHz to 6 GHz, being equal in the worst-case scenario to 0.981 W/kg (for the head), which is 9.81% of the ICNIRP limit (10 W/kg), and 0.008728 W/kg (for the whole-body average), which is 2.18% of the ICNIRP limit (0.4 W/kg). The 30 min average human core temperature rise is 0.055 °C, which is 5.5% of the ICNIRP limit. This indicates that, in typical automotive scenarios, the electromagnetic exposure from a monopole array antenna for V2V communications does not pose threat to the human body. This study provides knowledge related to emerging exposure scenarios in intelligent mobility communication, which is beneficial for evaluating possible health impacts and designing public health management policies. Full article
(This article belongs to the Section Vehicular Sensing)
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13 pages, 2219 KB  
Article
Worker Safety in High-Field NMR Spectroscopy Laboratories: Challenges and Risk Assessment
by Alessandra Flori, Giuseppe Acri, Maria Antonietta D’Avanzo, Massimo Mattozzi and Valentina Hartwig
Environments 2025, 12(4), 113; https://doi.org/10.3390/environments12040113 - 8 Apr 2025
Cited by 1 | Viewed by 2504
Abstract
Magnetic Resonance (MR) technology is extensively used in academic and industrial research laboratories and represents one of the most significant methodologies in clinical radiology. Although MR does not use ionizing radiation, it cannot be considered risk-free due to the strong static magnetic fields [...] Read more.
Magnetic Resonance (MR) technology is extensively used in academic and industrial research laboratories and represents one of the most significant methodologies in clinical radiology. Although MR does not use ionizing radiation, it cannot be considered risk-free due to the strong static magnetic fields and time-varying electromagnetic fields employed in the technology. To mitigate risks for MR operators, the European Community and ICNIRP have established safety limits based on the existing literature, primarily related to diagnostic MR. However, the literature on occupational exposure in non-clinical nuclear magnetic resonance (NMR) spectroscopy is limited. Due to their specificity, non-medical NMR environments present unique challenges from the point of view of operator exposure. NMR spectrometers are characterized by extremely high static magnetic fields, reaching up to 28 T in commercial systems; moreover, routine activities performed near the magnet, where field gradients are highest, increase operator exposure. Such environments are not typically perceived as hazardous and are frequented by various types of personnel, often without specific training. This study aims to highlight the critical issues in managing a preclinical MR laboratory equipped with a high-field NMR spectrometer, discussing operator safety challenges and presenting risk assessment data. Full article
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20 pages, 1843 KB  
Article
Assessment and Management of Risks from Occupational Exposure to Electromagnetic Fields (0 Hz to 300 GHz): A Compass to Keep the Right Course Through European and Italian Regulations
by Laura Filosa and Vanni Lopresto
Safety 2024, 10(4), 104; https://doi.org/10.3390/safety10040104 - 12 Dec 2024
Viewed by 5061
Abstract
This paper outlines the specific provisions of Italian legislation regarding workers’ exposure to electromagnetic fields (EMFs) from 0 Hz to 300 GHz compared to the minimum health and safety requirements set in European Directive 2013/35/EU. In particular, the path to be followed to [...] Read more.
This paper outlines the specific provisions of Italian legislation regarding workers’ exposure to electromagnetic fields (EMFs) from 0 Hz to 300 GHz compared to the minimum health and safety requirements set in European Directive 2013/35/EU. In particular, the path to be followed to assess and manage occupational exposure to EMFs is outlined in relation to the distinction between ‘professional’ and ‘non-professional’ exposure of workers, as well as to the precautionary limits regarding exposures from power lines (50 Hz) and broadcast and telecommunication fixed systems (100 kHz–300 GHz) established by Italian regulations. The reasons underlying such an approach—mainly relying on the intent to reconcile scientific evidence with risk perception in public opinion—are analysed and discussed with the aim of increasing the knowledge of national regulatory provisions on occupational risk assessment, which may be more stringent than the requirements envisaged by international guidelines and community regulations. Full article
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14 pages, 1582 KB  
Article
Delayed Growth in Immature Male Rats Exposed to 900 MHz Radiofrequency
by Raphaël Bodin, Franck Robidel, Stéphanie Rodrigues, Anthony Lecomte and Anne-Sophie Villégier
Appl. Sci. 2024, 14(16), 6978; https://doi.org/10.3390/app14166978 - 9 Aug 2024
Cited by 2 | Viewed by 1944
Abstract
People have been exposed to the 900 MHz mobile phone electromagnetic field for approximately 30 years. There is still no conclusion from immature rodent experiments regarding the potential effects of nonthermal radiofrequency (RF) 900 MHz continuous wave exposure during biological development. Here, we [...] Read more.
People have been exposed to the 900 MHz mobile phone electromagnetic field for approximately 30 years. There is still no conclusion from immature rodent experiments regarding the potential effects of nonthermal radiofrequency (RF) 900 MHz continuous wave exposure during biological development. Here, we test the hypothesis that mother rats exposed at a whole-body specific absorption rate (wbSAR) occupational (Oc) limit of the International Commission on Non-Ionizing Radiation Protection for humans (0.4 W/kg) will show impairments in development, with less effect at the public (Pu) limit (0.08 W/kg). The wbSAR was estimated at 0.4 W/kg to mimic working mothers (OcM exposure) and 0.08 W/kg for non-working mothers, i.e., public (PuM exposure). This pre- and postnatal study is the first to compare public and occupational exposure limits on rat pup physical development. Litter endpoints and the descendants’ body weights and lengths were recorded regularly from birth concomitantly with the age of developmental landmarks. Male neonates showed earlier pinna ear detachment and earlier eye opening in both the OcM and PuM groups, but earlier incisor eruption only in the PuM group. The OcM-exposed males showed lower body weight as juveniles until adolescence. The OcM- and PuM-exposed descendant females showed earlier pinna ear detachment and eye opening with similar body weight. These data suggest variations in the development time of descendant rats when the mother rats received daily 900 MHz continuous waves at human limits for workers and non-workers (public). Full article
(This article belongs to the Special Issue Electromagnetic Fields (EMF) Applications in Medicine)
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15 pages, 1079 KB  
Communication
Occupational Exposure to Electromagnetic Fields—Different from General Public Exposure and Laboratory Studies
by Kjell Hansson Mild, Mats-Olof Mattsson, Peter Jeschke, Michel Israel, Mihaela Ivanova and Tsvetelina Shalamanova
Int. J. Environ. Res. Public Health 2023, 20(16), 6552; https://doi.org/10.3390/ijerph20166552 - 9 Aug 2023
Cited by 14 | Viewed by 4207
Abstract
The designs of in vivo, in vitro and in silico studies do not adequately reflect the characteristics of long-term occupational EMF exposure; the higher exposure levels permitted for employees are nevertheless extrapolated on this basis. Epidemiological studies consider occupational exposure only in a [...] Read more.
The designs of in vivo, in vitro and in silico studies do not adequately reflect the characteristics of long-term occupational EMF exposure; the higher exposure levels permitted for employees are nevertheless extrapolated on this basis. Epidemiological studies consider occupational exposure only in a very general way, if at all. There is a lack of detailed descriptive data on long-term occupational exposure over the duration of the working life. Most studies reflect exposure characteristics of the general population, exposures which are long-term, but at a comparably low level. Occupational exposure is often intermittent with high peak power followed by periods with no exposure. Furthermore, the EU EMF-Directive 2013/35/EU states a demand for occupational health surveillance, the outcome of which would be of great help to epidemiologists studying the health effects of EMF exposure. This paper thus aims to outline and specify differences between public and occupational exposure and to increase the understanding of specific aspects of occupational exposure which are important for long-term health considerations. This could lead to a future protection concept against possible hazards based on adequate descriptions of long-term exposures and also include supplementary descriptive features such as a “reset time” of biological systems and accurate dose quantities. Full article
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16 pages, 5376 KB  
Article
Application of Metal Shielding Materials to Protect Buildings Occupants from Exposure to the Electromagnetic Fields
by Ervin Lumnitzer, Elena Lukac Jurgovska, Miriam Andrejiova and Ruzena Kralikova
Materials 2023, 16(15), 5438; https://doi.org/10.3390/ma16155438 - 3 Aug 2023
Cited by 8 | Viewed by 1889
Abstract
In recent decades, the background level of electromagnetic fields (EMFs) has increased extremely. One of the decisive factors influencing this increase is the increase in the quality, volume, and speed of voice and data services of mobile operators. This paper deals with the [...] Read more.
In recent decades, the background level of electromagnetic fields (EMFs) has increased extremely. One of the decisive factors influencing this increase is the increase in the quality, volume, and speed of voice and data services of mobile operators. This paper deals with the protection of the internal environment from the negative effects of EMFs through elements made of metal materials that absorb this radiation. For the purposes of this research, a series of measurements were carried out on individual days of the week and hours during the day. The results of the measurements were evaluated by the ANOVA method. The aim was to obtain a summary overview of the effects of electromagnetic fields and propose measures for their elimination in the interior. Therefore, measurements of electromagnetic fields were also carried out using shielding elements made of various metal materials, and a comparison of their shielding efficiency was subsequently made. Applications of shading blinds with the highest shading efficiency were recommended to increase safety, protect people’s health from its effects, and prevent electromagnetic fields. Full article
(This article belongs to the Special Issue Manufacturing Technology, Materials and Methods)
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18 pages, 2448 KB  
Project Report
NextGEM: Next-Generation Integrated Sensing and Analytical System for Monitoring and Assessing Radiofrequency Electromagnetic Field Exposure and Health
by Nikolaos Petroulakis, Mats-Olof Mattsson, Panos Chatziadam, Myrtill Simko, Andreas Gavrielides, Andrianos M. Yiorkas, Olga Zeni, Maria Rosaria Scarfi, Eduardo Soudah, Ruben Otin, Fulvio Schettino, Marco Donald Migliore, Andreas Miaoudakis, George Spanoudakis, John Bolte, Erdal Korkmaz, Vasileios Theodorou, Eleni Zarogianni, Susanna Lagorio, Mauro Biffoni, Andrea Schiavoni, Mauro Renato Boldi, Yuri Feldman, Igal Bilik, Anna Laromaine, Martí Gich, Marco Spirito, Maryse Ledent, Seppe Segers, Francisco Vargas, Loek Colussi, Mathieu Pruppers, Dan Baaken and Anna Bogdanovaadd Show full author list remove Hide full author list
Int. J. Environ. Res. Public Health 2023, 20(12), 6085; https://doi.org/10.3390/ijerph20126085 - 8 Jun 2023
Cited by 15 | Viewed by 5652
Abstract
The evolution of emerging technologies that use Radio Frequency Electromagnetic Field (RF-EMF) has increased the interest of the scientific community and society regarding the possible adverse effects on human health and the environment. This article provides NextGEM’s vision to assure safety for EU [...] Read more.
The evolution of emerging technologies that use Radio Frequency Electromagnetic Field (RF-EMF) has increased the interest of the scientific community and society regarding the possible adverse effects on human health and the environment. This article provides NextGEM’s vision to assure safety for EU citizens when employing existing and future EMF-based telecommunication technologies. This is accomplished by generating relevant knowledge that ascertains appropriate prevention and control/actuation actions regarding RF-EMF exposure in residential, public, and occupational settings. Fulfilling this vision, NextGEM commits to the need for a healthy living and working environment under safe RF-EMF exposure conditions that can be trusted by people and be in line with the regulations and laws developed by public authorities. NextGEM provides a framework for generating health-relevant scientific knowledge and data on new scenarios of exposure to RF-EMF in multiple frequency bands and developing and validating tools for evidence-based risk assessment. Finally, NextGEM’s Innovation and Knowledge Hub (NIKH) will offer a standardized way for European regulatory authorities and the scientific community to store and assess project outcomes and provide access to findable, accessible, interoperable, and reusable (FAIR) data. Full article
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16 pages, 3547 KB  
Article
50 Hz Magnetic Field Exposure Inhibited Spontaneous Movement of Zebrafish Larvae through ROS-Mediated syn2a Expression
by Yixin Guo, Yiti Fu and Wenjun Sun
Int. J. Mol. Sci. 2023, 24(8), 7576; https://doi.org/10.3390/ijms24087576 - 20 Apr 2023
Cited by 11 | Viewed by 3112
Abstract
Extremely low frequency electromagnetic field (ELF-EMF) exists widely in public and occupational environments. However, its potential adverse effects and the underlying mechanism on nervous system, especially behavior are still poorly understood. In this study, zebrafish embryos (including a transfected synapsin IIa (syn2a) overexpression [...] Read more.
Extremely low frequency electromagnetic field (ELF-EMF) exists widely in public and occupational environments. However, its potential adverse effects and the underlying mechanism on nervous system, especially behavior are still poorly understood. In this study, zebrafish embryos (including a transfected synapsin IIa (syn2a) overexpression plasmid) at 3 h post-fertilization (hpf) were exposed to a 50-Hz magnetic field (MF) with a series of intensities (100, 200, 400 and 800 μT, respectively) for 1 h or 24 h every day for 5 days. Results showed that, although MF exposure did not affect the basic development parameters including hatching rate, mortality and malformation rate, yet MF at 200 μT could significantly induce spontaneous movement (SM) hypoactivity in zebrafish larvae. Histological examination presented morphological abnormalities of the brain such as condensed cell nucleus and cytoplasm, increased intercellular space. Moreover, exposure to MF at 200 μT inhibited syn2a transcription and expression, and increased reactive oxygen species (ROS) level as well. Overexpression of syn2a could effectively rescue MF-induced SM hypoactivity in zebrafish. Pretreatment with N-acetyl-L-cysteine (NAC) could not only recover syn2a protein expression which was weakened by MF exposure, but also abolish MF-induced SM hypoactivity. However, syn2a overexpression did not affect MF-increased ROS. Taken together, the findings suggested that exposure to a 50-Hz MF inhibited spontaneous movement of zebrafish larvae via ROS-mediated syn2a expression in a nonlinear manner. Full article
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27 pages, 23056 KB  
Article
Physiological and Psychological Stress of Microwave Radiation-Induced Cardiac Injury in Rats
by Dayan Li, Xinping Xu, Yue Yin, Binwei Yao, Ji Dong, Li Zhao, Haoyu Wang, Hui Wang, Jing Zhang and Ruiyun Peng
Int. J. Mol. Sci. 2023, 24(7), 6237; https://doi.org/10.3390/ijms24076237 - 25 Mar 2023
Cited by 14 | Viewed by 4941
Abstract
Electromagnetic waves are widely used in both military and civilian fields, which could cause long-term and high-power exposure to certain populations and may pose a health hazard. The aim of this study was to simulate the long-term and high-power working environment of workers [...] Read more.
Electromagnetic waves are widely used in both military and civilian fields, which could cause long-term and high-power exposure to certain populations and may pose a health hazard. The aim of this study was to simulate the long-term and high-power working environment of workers using special electromagnetic radiation occupations to clarify the radiation-induced stress response and cardiac damage and thus gain insights into the mechanisms of injuries caused by electromagnetic radiation. In this study, the combination of microwave and stress was an innovative point, aiming to broaden the research direction with regard to the effect and mechanism of cardiac injury caused by radiation. The myocardial structure was observed by optical and transmission electron microscope, mitochondrial function was detected by flow cytometry, oxidative-stress markers were detected by microplate reader, serum stress hormone was detected by radioimmunoassay, and heart rate variability (HRV) was analyzed by multichannel-physiological recorder. The rats were weighed and subjected to an open field experiment. Western blot (WB) and immunofluorescence (IF) were used to detect the expressions and distributions of JNK (c-Jun N-terminal kinase), p-JNK (phosphorylated c-Jun N-terminal kinase), HSF1 (heat shock factor), and NFATc4 (nuclear factor of activated T-cell 4). This study found that radiation could lead to the disorganization, fragmentation, and dissolution of myocardial fibers, severe mitochondrial cavitation, mitochondrial dysfunction, oxidative-stress injury in myocardium, increase to stress hormone in serum, significant changes in HRV, and a slow gain in weight. The open field experiment indicated that the rats experienced anxiety and depression and had decreased exercise capacity after radiation. The expressions of JNK, p-JNK, HSF1, and NFATc4 in myocardial tissue were all increased. The above results suggested that 30 mW/cm2 of S-band microwave radiation for 35 min could cause both physiological and psychological stress damage in rats; the damage was related to the activation of the JNK pathway, which provided new ideas for research on protection from radiation. Full article
(This article belongs to the Special Issue Advances in Radiation Toxicity 2.0)
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