Search Results (1,821)

Damping-off caused by Fusarium spp. limits tomato seedling establishment, while chemical control is constrained by resistance development and environmental risks. As a result, biological alternatives and nanomaterials have attracted increasing interest. This study aimed to quantify the in vitro inhibition and in vivo control of Fusarium spp. associated with tomato damping-off using the marine strains KN1 and KN2 of Stenotrophomonas rhizophila and copper oxide nanoparticles (CuO-NPs). Nine fungal isolates were recovered from symptomatic plants; the most virulent isolate (3DR23HA) caused 60% disease incidence and was identified as Fusarium oxysporum. In dual-culture assays, both bacterial strains inhibited mycelial growth, with percentage inhibition of radial growth (PIRG) values exceeding 65% in several isolates, whereas KN1 showed greater inhibition of conidial germination. CuO-NPs exhibited a concentration-dependent response, reaching near-complete suppression at 0.75–1.0 mg mL⁻¹. In seedlings, the inoculated control showed 100% incidence and a disease severity index (DSI) of 85%, whereas KN1, KN1 + CuO-NPs at 0.75 mg mL⁻¹, and KN2 + CuO-NPs at 0.75 mg mL⁻¹ achieved a DSI of 0 and 100% control efficiency, while also improving growth and biomass. Overall, S. rhizophila, particularly strain KN1, and CuO-NPs at 0.75 mg mL⁻¹ represent a promising strategy for the integrated management of tomato damping-off in nurseries. Full article

This study evaluated the morphological development of 23 Coffea canephora clones in Espírito Santo to identify materials with superior vigor and quality for commercial and breeding purposes. Seedlings from cuttings were arranged in a completely randomized design with ten replicates and assessed at the commercial dispatch stage. Shoot and root growth, biomass, leaf area (LA), Dickson Quality Index (DQI), structural ratios (shoot/root ratio, SRR; height/diameter ratio, HDR), and anatomical traits were measured. Data were analyzed using analysis of variance with Scott–Knott clustering, Pearson correlation, and Principal Component Analysis (PCA). Significant variability was observed among clones. Clones 88, VR3, 8, and LB33 showed the highest stem diameter (SD), total dry mass (TDM), LA, and DQI, with balanced shoot and root development. Leaf area correlated strongly with SD, number of leaves (NL), biomass, and DQI, confirming its role as a seedling quality indicator. PCA identified two groups: a high-performance group with greater vigor and biomass, and a lower-performance group including clones 7, MR04, and VR4. The convergence of methods confirms the robustness of the results. Overall, clones 88, VR3, 8, and LB33 demonstrate superior agronomic potential at the seedling stage, offering promising options for nurseries, growers, and clonal selection programs. Full article

This study evaluated the effects of including spray-dried porcine plasma (SDP) in nursery diets and enzymatically hydrolyzed plasma (EHP) in drinking water on piglet growth performance and post-weaning diarrhea (PWD). Four treatments were tested: CONTROL (soy protein concentrate, SPC), P1SDP (5% SDP in phase 1), P1 + P2SDP (5% SDP and 2% SDP in phases 1 and 2), and EHP (0.88% in water during phases 1 and 2). No significant differences among treatments were observed during phase 1. During phase 2 (14–28 days) pigs fed SDP or pigs provided EHP in water had higher average daily gain (ADG; p = 0.001) and feed conversion (GFR; p = 0.013) versus the other groups. Pigs fed SDP in the first two phases had an average d-42 body weight that was 1.54 kg heavier than controls. Post-weaning diarrhea was not observed at any time during the study. These results support the use of SDP and EHP as effective nutritional strategies to enhance the growth and resilience of pigs during the post-weaning period. Both ingredients contribute to sustainable pig production by improving efficiency and promoting circular economy practices through the valorization of animal by-products. Full article

Coastal nurseries are critical for the early stages of many elasmobranchs, and understanding spatial ecology during these periods is essential for effective population management. Here, we investigated the environmental drivers shaping shark presence and spatial distribution in an open coastal nursery used by young-of-the-year Sphyrna lewini along the southern Pacific Coast of Mexico. Using acoustic telemetry data collected over three consecutive seasons, we combined Random Forest models with an interpretable machine learning framework, including permutation-based variable importance, accumulated local effects, and a Rashomon set approach. Shark presence was primarily driven by seasonal patterns and lunar illumination, whereas spatial distribution within the nursery area was structured by tide level, shark length, accumulated precipitation, and sea surface temperature. Tide level emerged as the most influential and stable predictor of spatial preference, while size-dependent responses revealed ontogenetic spatial segregation among zones. These results demonstrate that open-coast nurseries can operate through dynamic environmental processes rather than static habitat features, with river-influenced areas playing a key role for smaller individuals. By integrating telemetry data with interpretable machine learning methods, this study provides a mechanistic understanding of nursery habitat use and offers a transferable framework for assessing spatial ecology and conservation priorities in threatened coastal shark populations. Full article

This study evaluated the effect of different irrigation depths on soil water availability and vegetative growth of apricot (Prunus armeniaca L.) nursery plants under open-field conditions in northwestern Romania during the 2024 growing season. Two cultivars (‘Excelsior’ and ‘Favorit’) grafted on Prunus cerasifera were subjected to four irrigation regimes (0, 10, 20, and 30 mm per event). Soil moisture was monitored using tensiometers, and vegetative growth was assessed as total branch length. Results showed that irrigation significantly influenced soil water availability, especially during dry periods. Irrigated treatments maintained higher soil moisture compared to rainfed conditions. However, moderate irrigation levels (10–20 mm) produced vegetative growth comparable to higher irrigation (30 mm), indicating limited benefits of increasing irrigation depth beyond moderate levels. The Lp-norm index supported these findings by showing only small gains in integrated irrigation-growth performance at higher irrigation inputs. The study suggests that moderate irrigation can sustain vegetative growth in apricot nursery plants while reducing water use. These findings support the development of more efficient irrigation strategies, although results should be interpreted with caution as they are based on a single growing season. Full article

Efficient irrigation management is critical for improving irrigation water productivity and producing high-quality planting material in fruit tree nurseries. This study evaluated the effects of irrigation and fertilization on peach nursery performance through a two-year field experiment conducted in a commercial nursery in northwestern Romania. The experiment included two cultivars (‘Redhaven’ and ‘Cresthaven’), four irrigation depths (0, 10, 20, and 30 mm for each irrigation event), and two fertilization levels (N₀P₀K₀ and N₈P₈K₈) arranged in a factorial design. Irrigation significantly improved graft establishment and nursery survival compared to rainfed conditions. Optimal irrigation (20 mm) resulted in the highest nursery survival and provided the best balance between plant productivity and irrigation water productivity. Higher irrigation inputs increased total water consumption but reduced irrigation water productivity. Regression analysis revealed nonlinear relationships between water consumption and nursery performance, indicating diminishing returns at higher irrigation levels. The results suggest that moderate irrigation can enhance nursery productivity while improving water use efficiency. These findings provide practical guidance for optimizing irrigation strategies in commercial peach nursery systems. Full article

Pre-fattening (also referred to as nursery culture) of Manila clam is a priority for this sector of aquaculture, as it allows hatchery-produced seed (1–3 mg) to reach sowable juvenile sizes of 30–100 mg and reduces reliance on natural juvenile recruitment. This study evaluated the efficiency of two early pre-fattening systems, both in economic terms and in product quality: conventional upwelling units (a high-density system) and flat-bottom tanks (a mid-density system), the latter tested with and without a sand layer. The 51-day trial was conducted under autumn environmental conditions (temperature 13–25.8 °C; salinity 25–28 ppt; chlorophyll-a 3–24 µg/L), starting with 1.34 mg seed maintained under a water flow rate ≥ 15–20 mL/min/g. In upwelling units, the initial density was ~216 ind./cm². Four grading events produced four size classes, with total mean weights ranging from 6.4 mg in the smallest (tails) to 46.3 mg in the largest (heads). The overall population mean size was 19.0 mg, with a specific growth rate (SGR) of 5.2%/day and mortality of 17.6%. Flat-bottom tanks, stocked at ~30 ind./cm², achieved higher growth (overall weighted mean: 28.0 mg; SGR ~6%/day), but exhibited higher mortality (26.0% on average), with no significant effect from the presence of bottom sand. Overall, flat-bottom systems showed promising growth performance with reduced labor requirements, suggesting that this system could represent a viable alternative to upwelling. However, the associated rearing protocol could still be improved by optimizing stocking density and water exchange rates. Full article

To optimize container seedling cultivation of Chinese zelkova (Zelkova schneideriana Hand.-Mazz.), a three-factor completely randomized design was used to systematically evaluate the effects of container material, container size, and substrate composition on seedling growth, physiological traits, and root morphology. Different container materials, three container sizes, and multiple composite substrates were tested. Seedling height, biomass accumulation, photosynthetic characteristics, and root morphological indices were measured, and principal component analysis combined with comprehensive evaluation was applied to identify optimal treatments. The results showed that container size was one of the major factors affecting overall seedling quality, with large containers generally enhancing seedling height, biomass accumulation, photosynthetic capacity, and root development. Among container materials, B-type containers generally exhibited better overall performance under medium- and large-size conditions. Substrate composition showed a significant regulatory effect under appropriate container conditions, and the T₃ composite substrate, composed of yellow soil (40%), peat (10%), sphagnum peat (15%), vermiculite (10%), rice husk (15%), and corn cob (10%), achieved the highest comprehensive score. According to the PCA-based comprehensive evaluation, the T₃/A₃ treatment ranked first, followed by T₃/B₂. Overall, the combination of B-type containers, appropriate medium-to-large container size, and the T₃ substrate showed superior nursery performance. In particular, T₃/A₃ ranked first in the comprehensive evaluation, followed by T₃/B₂, indicating that both large black plastic containers and medium-sized B-type containers performed well under the T₃ substrate. Full article

To clarify cultivar differences in growth performance, yield formation, and bioactive characteristics at the sprout and fresh seed stages, twelve pea cultivars were evaluated. Growth traits, yield formation, and changes in phenolic compounds and antioxidant activity during sprouting were assessed, and the nutritional quality and mineral element composition of fresh seeds were also determined. The results showed that cultivars 24-164 and 24-510 exhibited low germination rates and severe cotyledon decay, making them unsuitable for sprout production. Significant differences were observed among the remaining cultivars in growth traits, edible ratio, and yield efficiency, with cultivars 24-724 and 24-486 showing superior processing efficiency and utilization value. During sprouting, total phenolic and total flavonoid contents, as well as 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP), were significantly influenced by both cultivar and light exposure stage. Root length and root diameter were significantly and positively correlated with phenolic accumulation and antioxidant activity. Analysis of fresh seed quality revealed marked inter-cultivar variation in nutritional and health-related traits. Cultivar 24-486 exhibited the highest values for phenolic content, antioxidant capacity, vitamin C, vitamin E, and Fe and Se accumulation, whereas cultivar 24-013 showed advantages in calcium and potassium contents. These results identify cultivars 24-724 and 24-486 as promising candidates for sprout production and highlight cultivar 24-486 as a dual-purpose genotype for both sprout and fresh seed utilization. Full article

We reported the first isolation and characterization of Neopestalotiopsis spp. from symptomatic strawberry plants in Nova Scotia, Canada. Morphological and multilocus sequence analyses confirmed that these isolates were closely related to previously identified aggressive Neopestalotiopsis spp. strains from strawberry and blueberry in the southeastern United States and other countries. Five representative isolates were evaluated for pathogenicity on detached leaves, whole plants, and fruits of multiple strawberry cultivars. The results revealed significant variation in virulence, with isolate NS-1 causing the most severe necrosis across all tissue types. Statistical analysis revealed significant effects of isolate, cultivar, and their interaction on disease severity, indicating differential cultivar responses to the tested isolates. Notably, tissue-specific differences were observed, with some isolates being aggressive on leaves but less virulent on fruit or whole plants, reinforcing the importance of multi-organ phenotyping in resistance screening. Phylogenetic analysis clustered the Nova Scotia isolates within the same clade as Neopestalotiopsis isolate 17–43 L from strawberry and isolates from blueberry, suggesting a potential epidemiological link. The shared nursery propagation system of strawberries and blueberries raises the risk of cross-infection, posing a substantial challenge to disease management strategies in both crops. Collectively, these findings underscore the urgent need for continued surveillance, population-level pathogen analysis, and the development of resistant cultivars to mitigate the spread of this emerging and rapidly evolving pathogen. Full article

Automated pig-welfare monitoring needs scalable, non-invasive signals that work across ages and individuals. A key methodological contribution of this study is the use of subject-wise validation, which ensures generalization to unseen animals and prevents inflated accuracy caused by growth-related and individual ‘voice’ differences. Vocalizations can help, but growth and individual “voice” differences can confound distress patterns and overstate accuracy without subject-wise validation. In our study, we explicitly accounted for individual variability by including animal identity as a random effect in mixed models and by using grouped cross-validation, where models were tested only on pigs not seen during training. This approach ensures that the reported accuracy reflects generalization across different individuals rather than memorization of specific vocal signatures. We analyzed 2221 vocal samples from 40 pigs (20 males, 20 females) recorded across four growth phases (farrowing, nursery, growing, finishing) under six conditions (pain, hunger, thirst, cold stress, heat stress, normal). Acoustic features extracted in Praat included energy, duration, intensity, pitch, and formants (F1–F4). Using blockwise variance decomposition, we quantified contributions of distress exposure, growth phase, and sex, and estimated the additional variance explained by animal identity. Distress exposure dominated intensity and spectral traits, particularly Formant 2, whereas the growth phase produced systematic shifts in duration and pitch. Animal identity added a modest but consistent increment in explained variance (~+0.02–0.03 R² beyond sex, phase, and distress). For prediction, we used 5-fold cross-validation grouped by animal. A Random Forest achieved a modest balanced accuracy of 0.609 and macro-F1 of 0.597; pain was most separable (recall 0.825), while other states showed moderate recall, indicating overlap. These results support hierarchical acoustic encoding of distress and establish a benchmark for precision welfare monitoring. Furthermore, they highlight that resolving complex physiological overlaps, such as heat stress and resource competition, requires a shift from unimodal acoustic models to multimodal Precision Livestock Farming (PLF) systems that integrate bioacoustics with continuous environmental and behavioral data streams. Full article

Using hedonic consumption theory (HCT) and a niche marketing strategy as analytical frameworks, our study examines consumer behavior in the luxury flower market, a swiftly growing segment of the global luxury goods industry. Adopting a nonexperimental, cross-sectional survey design, we collected primary data from 392 individuals from affluent households (defined as those with annual incomes exceeding $75,000, per standard demographic criteria) via purposive stratified sampling. Our questionnaire, which was reviewed by experts and tested in a pilot study, examined demographics, buying preferences, sustainability awareness, and hedonic motivations. The main findings show that most clients are well-educated women with substantial wealth. They care most about sensory, emotional, and symbolic qualities. Chi-square tests and logistic regressions robustly supported three hypotheses, gender disparities in appreciation, educational and sustainability awareness, and income influences on quality and variety emphasis, with descriptive evidence aligning with two further hypotheses regarding perceived supply shortages and sustainability preferences. The preferred places to buy include nurseries and high-end florists, suggesting opportunities for SMEs. Our study offers initial evidence supporting the application of HCT to perishable luxury floriculture among younger, educated, affluent consumers in North America. It underscores the hedonic appeal heightened by ephemerality and the potential influence of sustainability as a guilt-free enhancement, while indicating opportunities for niche marketing strategies through customization and sustainable sourcing. Our findings indicate opportunities for businesses aiming to reach comparable younger, educated, affluent demographics to fulfill unmet demand through sustainable sourcing, unique varieties, and customized experiences, which align with the SDGs. We conclude with a future research agenda. Full article

Cotinus coggygria (Scop) is a medicinally valuable species naturally distributed in the Artvin region of Turkiye. However, information on its seed traits, germination behavior, and seedling morphology in relation to seed size and provenance remains limited. This study aimed to evaluate the effects of seed size and provenance on the seed characteristics, germination, and seedling morphological traits of C. coggygria. Seeds were collected from four provenances (Seyitler, Tepekoy, Eskikale, and Tortum) and classified into large and small size groups using a 2 mm sieve. The seed traits of length, diameter, thickness, sphericity, volume, and thousand-seed weight were considered. To break seed dormancy, the seeds were subjected to sulfuric acid scarification and cold stratification treatments. Germination trials were conducted under nursery conditions using 45-cell trays in a randomized block design with four replicates. The mean germination time was significantly affected by provenance, whereas seed size and pretreatment combinations had no significant effects. Seed size did not significantly influence seedling morphology, whereas provenance caused significant differences. Seedlings originating from Eskikale exhibited greater height and root collar diameter, with root mass fractions ranging from 80.25% to 82.78%. These results indicate that provenance is a key factor influencing germination and seedling morphology rather than seed size. Full article

Phytophthora cinnamomi Rands, an oomycete pathogen of global relevance, is a major driver of cork oak (Quercus suber L.) decline and mortality in Mediterranean forests. Its management remains challenging in multifunctional landscapes where forestry and agriculture intersect, such as Mediterranean oak dehesas. Conventional fungicides are used against P. cinnamomi, but their negative environmental impacts underscore the need for alternative management in agroforestry systems. This study evaluated whether a commercially available microbial biostimulant, VESTA, enhances physiological performance and mitigates pathogen pressure in Q. suber. Seedlings were inoculated with P. cinnamomi and treated with the bioinoculant via fertigation or watering to substrate saturation, under controlled greenhouse conditions. Plant physiological parameters and soil oomycete inoculum concentrations were measured to assess treatment efficacy. Both application methods significantly improved physiological performance in inoculated and mock-inoculated plants. Photosynthesis, stomatal regulation, and water balance were most affected. Quantitative PCR analyses revealed a strong pathogen reduction, with DNA concentrations approximately tenfold lower in treated substrates (~0.001 ng mL⁻¹) than untreated controls (~0.011 ng mL⁻¹). Overall, the product enhanced Q. suber resilience by improving plant physiological responses and reducing pathogen abundance, supporting its potential as a bio-based tool for nurseries and restoration in Mediterranean ecosystems. Field studies are needed to validate these findings under natural variability and optimize long-term application strategies. Full article

Plant growth-promoting rhizobacteria (PGPR) are considered promising bio-inoculants for poplar production, but their effects can vary depending on bacterial strain, host genotype, and growth environment. In this study, we evaluated the responses of ten poplar clones representing three taxonomic groups to five indigenous PGPR strains under greenhouse and open-field nursery conditions. Under greenhouse conditions, Priestia aryabhattai GJRr2, Variovorax boronicumulans HNRr1, and a mixed inoculum (Mix) showed the most consistent positive effects. Plant height increased from 86.1 ± 5.6 cm in the control to 156.0 ± 9.4 cm in the GJRr2 treatment, whereas HNRr1 produced the greatest stem diameter (9.01 ± 0.26 mm) and total fresh weight (94.0 ± 6.0 g). Clone identity explained a larger independent fraction of growth variation than bacterial strain, and the strongest integrated responses were observed in I-476, Dorskamp, and Eco28. Field responses were generally weaker, but GJRr2 and Mix still increased height, DBH, and stem volume, whereas ORa was associated with negative responses in these traits. These results demonstrate that PGPR effects in poplar are strain-specific, clone-dependent, and environmentally contingent, indicating that inoculant selection should account for both host genotype and performance stability across growth conditions. Full article