Search Results (169)

Soil salinization severely limits plant growth and productivity. Mulberry (Morus alba L.), an economically and ecologically important tree, is widely cultivated, yet its salt-tolerance mechanisms at the seedling stage remain insufficiently understood. This study investigated the physiological and biochemical responses of two-year-old mulberry (‘Tailai Sang’) seedlings subjected to six NaCl treatments (0, 50, 100, 150, 200, and 300 mmol L⁻¹) for 28 days. Results showed that growth parameters and photosynthetic gas exchange exhibited dose-dependent declines. The reduction in net photosynthetic rate (Pn) was attributed to both stomatal limitations (decreased stomatal conductance) and non-stomatal limitations, as evidenced by a significant decrease in the maximum quantum efficiency of photosystem II (Fv/Fm) under high salinity. To cope with osmotic stress, seedlings accumulated compatible solutes, including soluble sugars, proteins, and proline. Critically, mulberry seedlings demonstrated effective ion homeostasis by sequestering Na⁺ in the roots to maintain a high K⁺/Na⁺ ratio in leaves, a mechanism that was compromised above 150 mmol L⁻¹. Concurrently, indicators of oxidative stress—malondialdehyde (MDA) and H₂O₂—rose significantly with salinity, inducing the activities of antioxidant enzymes (SOD, CAT, APX, and GR), which peaked at 150 mmol L⁻¹ before declining under extreme stress. A biomass-based LC₅₀ of 179 mmol L⁻¹ NaCl was determined. These findings elucidate that mulberry salt tolerance is a coordinated process involving three key mechanisms: osmotic adjustment, selective ion distribution, and a robust antioxidant defense system. This study establishes an indicative tolerance threshold under controlled conditions and provides a physiological basis for further field-based evaluations of ‘Tailai Sang’ mulberry for cultivation on saline soils. Full article

The Maowusu Desert is still suffering from serious ecological and environmental security issues such as wind erosion and desertification, influenced by both natural and human factors. The amendment of aeolian sandy soil with soft rock material presents an effective erosion control strategy, leveraging the complementary structural and compositional properties of both materials to enhance soil stability and rehabilitate degraded environments. However, there are few studies that investigate the effect of soil surface electrochemical properties and particle interaction forces on the structural stability of compound soils with soft rock and sandy soil. This decade-long field study quantified the electrochemical properties and interparticle forces and their synergistic effects on structural stability across five soft rock-to-aeolian sandy soil blend volume ratios (0:1, 1:5, 1:2, 1:1, 1:0) within the 0–30 cm soil profile. The results showed that the soil organic matter (SOM), specific surface area (SSA), and cation exchange capacity (CEC) significantly increased with the incorporation of soft rock material. For five different proportions, with the addition of soft rock and the extension of planting years, the content of SOM increased from 5.65 g·kg⁻¹ to 11.36 g·kg⁻¹, the CEC varied from 4.68 cmol kg⁻¹ to 17.91 cmol kg⁻¹, while the σ₀ importantly decreased from 1.8 to 0.47 c m⁻² (p < 0.05). For the interaction force at 2.4 nm between soil particles, the absolute value of van der Waals attractive force increased from 0.10 atm to 0.38 atm, and the net force decreased from 0.09 atm to −0.30 atm after the incorporation ratios of soft rock from 0:1 to 1:1. There was a significant negative correlation between the resultant net force between the particles of compound soil and the SSA and CEC. These results indicate that the addition of soft rock material positively improves the surface electrochemical properties and internal forces between aeolian sandy soil particles, further enhancing its structural stability. This study establishes a foundational theoretical framework for advancing our mechanistic understanding of aeolian sand stabilization and ecosystem rehabilitation in the Mu Us Desert. Full article

Typhoons are major climate disturbances that significantly impact coastal ecosystems, particularly mangrove forests. This study examines the effects of typhoons on mangrove communities at different stages of recovery, focusing on how environmental factors influence carbon storage and net ecosystem exchange (NEE). Three mangrove sites were selected based on their recovery age: young, moderately restored, and mature. The results revealed that typhoons had the most pronounced effect on young mangroves, resulting in significant reductions in both above-ground and soil carbon storage. In contrast, mid-aged and mature mangroves demonstrated greater resilience, with mature mangroves recovering most rapidly in terms of community structure and carbon storage. Key factors such as wind speed, heavy rainfall, and changes in photosynthetically active radiation (PAR) contributed to carbon storage losses, particularly in young mangrove forests. This study underscores the importance of recovery age in determining mangrove resilience to extreme weather events and offers insights for enhancing restoration and conservation strategies to mitigate the impacts of climate change on coastal carbon sequestration. Full article

Land degradation from trace metal pollution in North Africa severely compromises soil fertility. This study investigates the synergistic remediation potential of Eucalyptus biochar (EuB) and Casuarina glauca in iron mine soil contaminated with Fe, Zn, Mn, Pb, Cd, and As. Seedlings were grown for six months in: non-mining soil (NMS), contaminated soil (CS), and CS amended with 5% EuB (CS + EuB). Comprehensive ecophysiological assessments evaluated growth, water relations, gas exchange, chlorophyll fluorescence, oxidative stress, and metal accumulation. EuB significantly enhanced C. glauca tolerance to multi-trace metal stress. Compared to CS, CS + EuB increased total dry biomass by 14% and net photosynthetic rate by 22%, while improving predawn water potential (from −1.8 to −1.3 MPa) and water-use efficiency (18%). Oxidative damage was mitigated. EuB reduced soluble Fe by 71% but increased Zn, Mn, Pb, and Cd mobility. C. glauca exhibited hyperaccumulation of Fe, Zn, As, Pb, and Cd across treatments, with pronounced Fe accumulation under CS + EuB. EuB enhanced nodule development and amplified trace metals sequestration within nodules (Zn: +1.4×, Mn: +2.4×, Pb: +1.5×, Cd: +2.0×). The EuB-C. glauca synergy enhances stress resilience, optimizes rhizosphere trace metals bioavailability, and leverages nodule-mediated accumulation, establishing a sustainable platform for restoring contaminated lands. Full article

Reclaiming coastal wetlands for agricultural purposes has led to intensified farming activities, which are anticipated to affect greenhouse gas (GHG) flux processes within coastal wetland ecosystems. However, how greenhouse gas exchanges respond to variations in agricultural reclamation activities across different years remains uncertain. To address this knowledge gap, this study characterized dynamic exchanges within the soil–plant–atmosphere continuum by employing continuous monitoring across four representative coastal wetland soil–vegetation systems in Jiangsu, China. The results show the carbon dioxide (CO₂) and nitrous oxide (N₂O) flux exchanges between the system and the atmosphere and soil–vegetation carbon pools, which revealed the drivers of carbon dynamics in the coastal wetland system. The four study sites, converted from coastal wetlands to agricultural lands at different times (years), generally act as CO₂ sinks and N₂O sources. Higher levels of CO₂ sequestration occur as the age of reclamation rises. In terms of time scale, crops lands were found to be CO₂ sinks during the growing period but became CO₂ sources during the crop fallow period. Although the temporal trend of the N₂O flux was generally smooth, reclaimed farmlands acted as net sources of N₂O, particularly during the crop-growing period. The RDA and PLS-PM models illustrate that soil salinity, acidity, and hydrothermal conditions were the key drivers affecting the magnitude of the GHG flux exchanges under reclamation. This study demonstrates that GHG emissions from reclaimed wetlands can be effectively regulated through science-based land management, calling for prioritized attention to post-development practices rather than blanket restrictions on coastal exploitation. Full article

The potato (Solanum tuberosum L.) is highly dependent on water availability, with physiological sensitivity varying throughout its phenological cycle. In the context of increasing water scarcity and greater climate variability, identifying critical periods where water stress negatively impacts productivity and tuber quality is essential. This study evaluated the physiological response of potatoes under different deficit irrigation strategies in field conditions, and aimed to determine the irrigation reduction thresholds that optimize water use efficiency without significantly compromising yield. Five irrigation regimes were applied: well-watered (T1; irrigation was applied when the volumetric soil moisture content was close to 35% of total water available), 130% of T1 (T2, 30% more than T1), 75% of T1 (T3), 50% of T1 (T4), and 30% of T1 (T5). Key physiological parameters were monitored, including gas exchange (net photosynthesis, stomatal conductance, and transpiration), chlorophyll fluorescence (Fv’/Fm’, ΦPSII, electron transport rate), and photosynthetic pigment content, at three critical phenological phases: tuberization, flowering, and fruit set. The results indicate that water stress during tuberization and flowering significantly reduced photosynthetic efficiency, with decreases in stomatal conductance (gs), effective quantum efficiency of PSII (ΦPSII), and electron transport rate (ETR). In contrast, moderate irrigation reduction (75%) lowered the seasonal application of water by ~25% (≈80 mm ha⁻¹) while maintaining commercial yield and tuber quality comparable to the fully irrigated control. Intrinsic water use efficiency increased by 18 ± 4% under this regime. These findings highlight the importance of irrigation management based on crop phenology, prioritizing water supply during the stages of higher physiological sensitivity and allowing irrigation reductions in less critical phases. In a scenario of increasing water limitations, this strategy enhances water use efficiency while ensuring the production of tubers with optimal commercial quality, promoting more sustainable agricultural management practices. Full article

Coal gangue is a fine-grained mineral with nutrient content, which can be used as a potential soil amendment. Nevertheless, current research on using coal gangue to improve soil water and support plant growth is still insufficient. In this study, coal gangue powder (CGP) was added to aeolian sandy soil. We compared the soil hydraulic properties and plant growth of original aeolian sandy soil (CK) and different CGP application rates (10% and 20%). The results indicated that the application of CGP transformed the soil texture from sandy to loamy, significantly reduced soil bulk density and saturated hydraulic conductivity (Ks) values, altered the soil water characteristic curve, enhanced soil water-holding capacity, and increased plant-available water. Compared with the CK group, the emergence rate of alfalfa seeds increased from approximately 50% to over 70% after CGP application. During the growth process, CGP application significantly elevated the net photosynthetic rate, transpiration rate, and stomatal conductance of alfalfa leaves. Rapid fluorescence kinetics monitoring of leaves demonstrated that alfalfa treated with CGP had a higher efficiency in light energy utilization. However, the photosynthetic capacity of leaves did not improve as the CGP application rate increased from 10% to 20%, suggesting that excessive CGP addition did not continuously benefit plant gas exchange. In conclusion, CGP application can improve the soil hydraulic properties of aeolian sandy soil and support plant growth and development, which is conducive to reducing the accumulated amount of coal gangue, alleviating plant water stress, and promoting ecological restoration in arid mining areas. We recommend a 10% addition of coal gangue powder as the optimal amount for similar soils. Full article

Plants are commonly exposed to fluctuating illumination under natural light conditions, causing dynamic photosynthesis and further affecting plant growth and productivity. In this context, although the vital role of potassium (K) in steady-state photosynthesis has been well-established, knowledge of the dynamic changes in photosynthesis mediated by K remains scarce. Here, the gas-exchange and chlorophyll fluorescence parameters under steady-state and dynamic photosynthetic responses were quantified in Phaseolus vulgaris L. seedlings grown under K-deficient (−K, 0.02 mM K) and normal K (+K, 2 mM K) conditions. After a transition from low to high light, the time course–induction curves of the net photosynthetic rate (A), stomatal conductance (g_s), mesophyll conductance (g_m), and maximum carboxylation rate (V_cmax) showed an obvious decline in the −K treatment. In comparison with the +K treatment, however, there were no statistical differences in the initial A and V_cmax values in P. vulgaris supplied with deficient K, suggesting that the K-deficiency-induced decreases in A and V_cmax were light-dependent. Interestingly, the time to reach 90% of the maximum A, g_s, and g_m significantly decreased in the −K treatment in comparison with the +K treatment by 27.2%, 45.6%, and 52.9%, respectively, whereas the time to reach 90% of the maximum V_cmax was correspondingly delayed by almost two-fold. The photosynthetic limitation during the induction revealed that the biochemical limitation was the dominating factor that constrained A under the −K conditions, while, under the +K conditions, the main limiting factor changed from biochemical limitation to stomatal limitation over time. Moreover, g_m imposed the smallest limitation on A during induction in both K treatments. These results indicate that a decreased K supply decreases the photosynthetic performance under fluctuating light in P. vulgaris and that improving the induction responses of biochemical components (i.e., V_cmax) has the potential to enhance the growth and productivity of crops grown in K-poor soil. Full article

Agroecosystems play a key role in the global carbon cycle, with CO₂ exchange driven by photosynthesis and respiration. Indicators such as gross primary productivity (GPP), net primary productivity (NPP), and carbon, water, and light use efficiency (CUE, WUE, LUE) are essential for assessing resource use in agricultural systems. Conventional tillage depletes carbon, water, and nutrients, negatively impacting the environment, while conservation practices aim to improve soil health and biodiversity. This study evaluated the effects of a cover crop in a wheat–maize rotation on sloped arable land prone to water erosion. The experiment involved minimum contour tillage combined with cover cropping, and its impact on carbon balance components and resource use efficiency was assessed. The results demonstrated that the inclusion of a cover crop significantly improved GPP and NPP. Water and light use efficiency also increased, particularly in 2022 and 2023, which were characterized by summer drought. However, carbon use efficiency remained unchanged over the study period. These findings highlight the potential of conservation practices, such as cover cropping and reduced tillage, to enhance productivity and resource efficiency in sloped agricultural landscapes under water stress conditions. Full article

Phosphorus (P) is essential for crop growth, but its complex behavior in tropical soils necessitates alternative management strategies, such as foliar supplementation. Foliar-applied nutrients act as biostimulants, enhancing stress tolerance and plant productivity. This study assessed the physiological responses of soybean to foliar application of soluble monoammonium phosphate (MAP; at a rate of 5 kg ha⁻¹ each application) at different phenological stages (two during vegetative stages V₄ and V₆ and two during reproductive stages R₁ and R₃ or all four stages) across two growing seasons in tropical field conditions. Key parameters analyzed included leaf nutrient content, photosynthetic pigments, Rubisco activity, carbohydrate content, gas exchange (photosynthetic rate, stomatal conductance, transpiration, water use efficiency, and carboxylation efficiency), oxidative stress markers, and productivity indicators (100-grain weight and grain yield). MAP application improved all parameters, particularly at R₁ and R₃. Total chlorophyll increased by 29.2% at R₁ and 30.0% when applied at all four stages, while the net photosynthetic rate rose by 15.8% and 18.4%, respectively. Water use efficiency improved by 20.0% at R₁ and all four stages, while oxidative stress indicators, such as H₂O₂ levels, decreased. Rubisco activity increased most at R₃ (46.0%) and all four stages (59.9%). Grain yield was highest with MAP spread at all four stages (12.3% increase), though a single application at R₁ still boosted yield by 7.4%, compared to the control treatment. Full article

The carbon cycle in terrestrial ecosystems is a crucial component of the global carbon cycle, and drought is increasingly recognized as a significant stressor impacting their carbon sink function. Net ecosystem productivity (NEP), which is a key indicator of carbon sink capacity, is closely related to vegetation Net Primary Productivity (NPP), derived using the Carnegie-Ames-Stanford Approach (CASA) model. However, there is limited research on desert grassland ecosystems, which offer unique insights due to their long-term data series. The relationship between NEP and drought is complex and can vary depending on the intensity, duration, and frequency of drought events. NEP is an indicator of carbon exchange between ecosystems and the atmosphere, and it is closely related to vegetation productivity and soil respiration. Drought is known to negatively affect vegetation growth, reducing its ability to sequester carbon, thus decreasing NEP. Prolonged drought conditions can lead to a decrease in vegetation NPP, which in turn affects the overall carbon balance of ecosystems. This study employs the improved CASA model, using remote sensing, climate, and land use data to estimate vegetation NPP in desert grasslands and then calculate NEP. The Standardized Precipitation Evapotranspiration Index (SPEI), based on precipitation and evapotranspiration data, was used to assess the wetness and dryness of the desert grassland ecosystem, allowing for an investigation of the relationship between vegetation productivity and drought. The results show that (1) from 1982 to 2022, the distribution pattern of NEP in the Inner Mongolia desert grassland ecosystem showed a gradual increase from southwest to northeast, with a multi-year average value of 29.41 gCm⁻². The carbon sink area (NEP > 0) accounted for 67.99%, and the overall regional growth rate was 0.2364 gcm⁻²yr⁻¹, In addition, the area with increasing NEP accounted for 35.40% of the total area (p < 0.05); (2) using the SPEI to characterize drought changes in the Inner Mongolia desert grassland ecosystems, the region as a whole was mainly affected by light drought. Spatially, the cumulative effect was primarily driven by short-term drought (1–2 months), covering 54.5% of the total area, with a relatively fast response rate; (3) analyzing the driving factors of NEP using the Geographical detector, the results showed that annual average precipitation had the greatest influence on NEP in the Inner Mongolian desert grassland ecosystem. Interaction analysis revealed that the combined effect of most factors was stronger than the effect of a single factor, and the interaction of two factors had a higher explanatory power for NEP. This study demonstrates that NEP in the desert grassland ecosystem has increased significantly from 1982 to 2022, and that drought, as characterized by the SPEI, has a clear influence on vegetation productivity, particularly in areas experiencing short-term drought. Future research could focus on extending this analysis to other desert ecosystems and incorporating additional environmental variables to further refine the understanding of carbon dynamics under drought conditions. This research is significant for improving our understanding of carbon cycling in desert grasslands, which are sensitive to climate variability and drought. The insights gained can help inform strategies for mitigating climate change and enhancing carbon sequestration in arid regions. Full article

Net ecosystem productivity (NEP) is a crucial metric for quantifying carbon storage, exchange, and cycling across global atmospheric and terrestrial ecosystems. This study examines the spatiotemporal patterns of NEP in China’s Zoigê alpine grassland and its response to climate variability, phenological changes, and soil conditions from 2000 to 2020. The results show a statistically significant increase in the annual NEP of the Zoigê Plateau, with an average rate of 3.18 g C/m²/year. Spatially, NEP displays strong heterogeneity, with higher values in the southwestern and northeastern marginal areas (>80 g C/m²) and lower values in the central region (<0 g C/m²). In alpine meadows (standardized total effect coefficient [STEC] = 0.52) and alpine steppes (STEC = 0.43), NEP is primarily regulated by soil moisture modulation, influenced by both water and temperature factors. This study accurately assesses NEP by incorporating regional soil characteristics, providing a more precise evaluation of changes in vegetation carbon sink sources in high-altitude areas. Full article

Background and Methods: We assessed the prokaryotic and eukaryotic diversity present in non-vegetated and vegetated soils on King George Island, Maritime Antarctic, in combination with measurements of carbon dioxide fluxes. Results: For prokaryotes, 381 amplicon sequence variants (ASVs) were assigned, dominated by the phyla Actinobacteriota, Acidobacteriota, Pseudomonadota, Chloroflexota, and Verrucomicrobiota. A total of 432 eukaryotic ASVs were assigned, including representatives from seven kingdoms and 21 phyla. Fungi dominated the eukaryotic communities, followed by Viridiplantae. Non-vegetated soils had higher diversity indices compared with vegetated soils. The dominant prokaryotic ASV in non-vegetated soils was Pyrinomonadaceae sp., while Pseudarthrobacter sp. dominated vegetated soils. Mortierella antarctica (Fungi) and Meyerella sp. (Viridiplantae) were dominant eukaryotic taxa in the non-vegetated soils, while Lachnum sp. (Fungi) and Polytrichaceae sp. (Viridiplantae) were dominant in the vegetated soils. Measured CO₂ fluxes indicated that the net ecosystem exchange values measured in vegetated soils were lower than ecosystem respiration in non-vegetated soils. However, the total flux values indicated that the region displayed positive ecosystem respiration values, suggesting that the soils may represent a source of CO₂ in the atmosphere. Conclusions: Our study revealed the presence of rich and complex communities of prokaryotic and eukaryotic organisms in both soil types. Although non-vegetated soils demonstrated the highest levels of diversity, they had lower CO₂ fluxes than vegetated soils, likely reflecting the significant biomass of photosynthetically active plants (mainly dense moss carpets) and their resident organisms. The greater diversity detected in exposed soils may influence future changes in CO₂ flux in the studied region, for which comparisons of non-vegetated and vegetated soils with different microbial diversities are needed. This reinforces the necessity for studies to monitor the impact of resident biota on CO₂ flux in different areas of Maritime Antarctica, a region strongly impacted by climatic changes. Full article

The performance of a conventional Ground-Source Refrigeration and Air Conditioning (GSRAC) system with a borehole heat exchanger (BHE) can be enhanced by addressing the soil thermal imbalance issue that affects these systems. This study proposes a novel concept for seasonal cold energy storage using a Thermal Diode Tank (TDT). The TDT consists of an insulated water tank fitted with an array of heat pipes. By integrating the TDT into a conventional GSRAC system, “cold” energy can be passively collected from ambient air during winter, injected into the BHE, and stored in the soil. The stored “cold” energy can then be retrieved in the summer, facilitating cross-seasonal cold energy storage (CS). Thus, a conventional GSRAC system can be transformed into a GSRAC system with cross-seasonal cold energy storage capability, i.e., GSRAC + CS system. The validated BHE model previously developed by the authors is used to predict the performance improvements achieved using the GSRAC + CS system. The results indicate that the Annual Net Cold Energy Storage Efficiency (ANESE) increased from 5.7% to 10.7% over a ten year period. The average Borehole Performance Improvement (BPI) due to the addition of cold storage capability is 11% over the same timeframe. This study also discusses the impacts of varying design and operational parameters on ANESE and BPI. The results demonstrate that GSRAC + CS systems not only mitigate the soil thermal imbalance issue faced by conventional GSRAC systems, but also require less BHE depth to achieve equivalent performance. Full article

Many agricultural fields are no longer sustainable due to inadequate replenishment of soil nutrients through organic and inorganic inputs, particularly in smallholder farming systems. As a result, achieving potential crop yields in these systems has proven to be difficult. Field trials were conducted in two long rainy growing seasons in 2021 and 2023 to assess the effects of urea fertilizer and cattle manure as sources of nitrogen (N) on (i) maize crop yields and (ii) soil chemical properties at two sites (Kwa Sadala and Mungushi) located in Hai district, northern Tanzania. The trials employed a randomized complete block design with three replicates, including eight treatments. The treatments were: 0 fertilizer (control), 25, 50, 75 kg N ha⁻¹ (sole urea), 12.5 kg N (urea) + 12.5 kg N (cattle manure), 25 kg N (urea) + 25 kg N (cattle manure), and 50 and 75 kg N (sole cattle manure). Results show that the highest application rate of urea (75 kg N ha⁻¹) produced the highest grain yields of 4.21 and 4.09 t ha⁻¹ in the 2021 season and 4.32 and 4.04 t ha⁻¹ in the 2023 season at Kwa Sadala and Mungushi, respectively. The application of cattle manure at the highest rates increased the soil pH by 3.15 and 2.26% at Kwa Sadala and Mungushi, respectively. Similarly, soil total N, OC, available/extractable P, and exchangeable K increased by 100%, 56.3%, 52.36%, and 19.67%, respectively, at Kwa Sadala and by 16.67%, 18.13%, 20.95%, and 6.76%, respectively, at Mungushi. The use of urea alone at the higher rates or in combination with cattle manure at 50% each resulted in the highest net benefit (NB) in all sites. The findings from this study suggest that a comprehensive approach to managing soil nutrients, such as combining inorganic and organic inputs, may improve crop yields while maintaining soil health. Full article