Search Results (40)

Medication-related osteonecrosis of the jaw (MRONJ) is a serious adverse effect of antiresorptive and antiangiogenic therapies, yet its molecular mechanisms remain poorly defined. The present study employed an analysis of microarray data (GSE7116) from peripheral blood mononuclear cells of patients with multiple myeloma, myeloma patients with MRONJ, and healthy controls. Differentially expressed genes were identified using the limma package, followed by functional enrichment analysis, weighted gene co-expression network analysis, and LASSO regression and CytoHubba network ranking. The predictive performance was validated by means of nested cross-validation, Firth logistic regression, and safe stratified 0.632+ bootstrap ridge regression. The profiling revealed distinct gene expression patterns between the groups: the upregulation of ribosomal and translational pathways, as well as the suppression of neutrophil degranulation and antimicrobial defense mechanisms, and identified key candidate genes, including PDE4B, JAK1, ETS1, EIF4A2, FCMR, IGKV4-1, and XPO7. These genes demonstrated substantial discriminatory capability, with an area under the curve ranging from 0.95 to 0.99, and were found to be functionally linked to immune system dysfunction, cytokine signaling, NF-κB activation, and a maladaptive stress response. These findings link MRONJ to systemic immune-inflammatory imbalance and translational stress disruption, offering novel insights and potential biomarkers for diagnosis and risk evaluation. Full article

Nest defense is a key component of avian reproductive success, yet its intensity and expression often depend on ecological and social contexts. We investigated the nest defense behaviors of Saunders’s Gulls (Saundersilarus saundersi) breeding in Incheon Bay of South Korea in 2022 in relation to nest density and perceived threats. Using decoy presentations of three heterospecifics, Oriental Magpie (Pica serica; diurnal avian nest predator), common raccoon dog (Nyctereutes procyonoides; nocturnal mammalian nest predator), and Little Tern (Sternula albifrons; neutral co-nester), we quantified latency to respond, bombing attack rate, and the number of mobbing individuals at high- and low-density nesting sites within a breeding colony. Mixed models revealed that latency to respond and attack rates varied strongly with stimulus type, with diurnal predator magpies eliciting the fastest and most intense responses, followed by nocturnal predator raccoon dogs and co-nester terns. Nest density influenced the number of mobbing individuals, which was significantly greater at high-density sites. Principal Component Analysis reduced the three behavioral metrics into a composite score, which correlated negatively with latency and positively with bombing attack rate and mobbing intensity. This score varied with both nest density and stimulus type. Our findings demonstrate that Saunders’s Gulls adjust their nest defense strategies according to both the social context and predator type, highlighting the importance of density-dependent collective nest defense in colonial breeders. Full article

Antipredator behavior can vary consistently among individuals, yet links between proactive nest defense and passive fear strategies are rarely quantified in the wild. We tested whether hissing, a conspicuous, snake-like display at the nest, predicts tonic immobility (TI) and breeding success in female Great Tits (Parus major). In pine forests in southeastern Latvia (2023–2024), we presented a taxidermic Great Spotted Woodpecker (Dendrocopos major) at nest-box entrances during incubation and scored whether females hissed and how many calls they produced. The same females were later assayed for TI by brief supine restraint when nestlings were 3–4 days old. Of 141 incubating females, 105 (74.5%) hissed. TI duration differed sharply between groups: non-hissing females showed significantly longer TI than hissing females. Nest failure was significantly lower in hissing than non-hissing female nests. These results reveal a strong negative association between proactive defense and passive fearfulness, and they show that hissing can translate into higher reproductive success in a Woodpecker-dominated predator environment. We conclude that defense strategies covary within individuals along a personality axis and that predator community composition may shape selection on these strategies. Full article

The Intel^® Trust Domain Extensions (TDX) encrypt guest memory and minimize host interactions to provide hardware-enforced isolation for sensitive virtual machines (VMs). Software vulnerabilities in the guest OS continue to pose a serious risk even as the TDX improves security against a malicious hypervisor. We suggest a comprehensive TDX Guest Fuzzing Framework that systematically explores the guest’s code paths handling untrusted inputs. Our method uses a customized coverage-guided fuzzer to target those pathways with random input mutations following integrating static analysis to identify possible attack surfaces, where the guest reads data from the host. To achieve high throughput, we also use snapshot-based virtual machine execution, which returns the guest to its pre-interaction state at the end of each fuzz iteration. We show how our framework reveals undiscovered vulnerabilities in device initialization procedures, hypercall error-handling, and random number seeding logic using a QEMU/KVM-based TDX emulator and a TDX-enabled Linux kernel. We demonstrate that a large number of vulnerabilities occur when developers implicitly rely on values supplied by a hypervisor rather than thoroughly verifying them. This study highlights the urgent need for ongoing, automated testing in private computing environments by connecting theoretical completeness arguments for coverage-guided fuzzing with real-world results on TDX-specific code. We discovered several memory corruption and concurrency weaknesses in the TDX guest OS through our coverage-guided fuzzing campaigns. These flaws ranged from nested #VE handler deadlocks to buffer overflows in paravirtual device initialization to faulty randomness-seeding logic. By exploiting these vulnerabilities, the TDX’s hardware-based memory isolation may be compromised or denial-of-service attacks may be made possible. Thus, our results demonstrate that, although the TDX offers a robust hardware barrier, comprehensive input validation and equally stringent software defenses are essential to preserving overall security. Full article

Between 14 and 16 June 2023, an extratropical cyclone affected the south-southeastern coast of Brazil, causing significant damage and loss of life. In the state of Rio Grande do Sul, Civil Defense authorities reported at least 16 fatalities. Although numerical models can simulate the general characteristics of extratropical cyclones, they often struggle to accurately represent the intensity and timing of strong winds and heavy precipitation. One approach to improving such simulations is the use of convective-permitting models (CPMs), in which convection is explicitly resolved. In this context, the main objective of this study is to assess the performance of the Weather Research and Forecasting (WRF) model in CP mode, nested in the ERA5 reanalysis, in representing both the synoptic and mesoscale structures of the cyclone, as well as its associated strong winds and precipitation. The WRF-CP successfully simulated the cyclone’s track, though with some discrepancies in the cyclone location during the first 12 h. Comparisons with radar-based precipitation estimates indicated that the WRF-CP captured the location of the observed precipitation bands. During the cyclone’s occlusion phase—when precipitation was particularly intense—hourly simulated precipitation and 10 m wind (speed, zonal, and meridional components) were evaluated against observations from meteorological stations. WRF-CP demonstrated strong skill in simulating both the timing and intensity of precipitation, with correlation coefficients exceeding 0.4 and biases below 0.5 mm h⁻¹. Some limitations were observed in the simulation of 10 m wind speed, which tended to be overestimated. However, the model performed well in simulating the wind components, particularly the zonal component, as indicated by predominantly high correlation values (most above 0.4), suggesting a good representation of wind direction, which is a function of the zonal and meridional components. Overall, the simulation highlights the potential of WRF-CP for studying extreme weather events, including the small-scale structures embedded within synoptic-scale cyclones responsible for producing adverse weather. Full article

In 21st-century American cities, urban crime remains a critical public safety concern influenced by complex social, political, and environmental structures. Crime is not randomly distributed and built-environment characteristics, such as street network configuration, impact criminal activity through spatial dependence effects at multiple scales. This study investigates the cross-sectional, multi-scale spatial effects of street network configuration on property crime across neighborhoods in Charlotte, North Carolina. Specifically, we examine whether the fundamental characteristics of a neighborhood’s street network contribute to variations in its property crime. Using a novel and granular spatial approach, incorporating spatial econometric models (SAR, CAR, and GWR), several street network characteristics, including density, connectivity, and centrality, within five nested buffer bands are measured to capture both local and non-local influences. The results provide strong and consistent evidence that certain characteristics of the neighborhood street network, such as connectivity and accessibility, significantly influence the occurrence of property crime. Impacts are also found to be spatially heterogenous, manifesting themselves at the mid-range scale rather than hyper-locally. The integration of comprehensive measures of street network configuration into spatially explicit models offers new opportunities for advancement in environmental criminology literature. Such spatial dynamics further contribute to urban safety policy by informing decision-makers so that they can ensure a defensively built environment design. Full article

On the beach of Linosa Island (Italy), 43 loggerhead sea turtle (Caretta caretta) unhatched eggs were recovered from nests, formalin-fixed and necropsied. The tissue samples were stained with hematoxylin-eosin (HE), Grocott, von Kossa, periodic acid-Schiff (PAS), and Movat pentachrome stains. Histologically, vacuolar degeneration (100.0%) and increased numbers of melanomacrophages (18.6%) in the liver, and edema (14.0%) in the lungs were observed. Twenty-five kidneys (58.1%) showed deposition of blue amorphous material with HE staining, which also appeared PAS-positive and black with von Kossa staining, allowing a diagnosis of calcium oxalate, confirmed by transmission electron microscopy (TEM). The hepatic lesions may be indicative of toxicosis, infection, or a defense mechanism. A statistically significant association between the nest position and renal oxalosis (renal calcium oxalate deposition) was observed. Renal oxalosis was probably due to the exceptionally high summer temperatures, which were statistically higher compared to the temperatures recorded in the previous two years. Full article

Termites are eusocial insects with functionally specialized workers and soldiers, both sharing the same genotype. Additionally, lower termites host flagellates in their hindguts that assist in wood digestion. However, worker-biased and soldier-biased gene expression patterns of the host–flagellate symbiotic system remain underexplored in most taxonomic groups. In this study, we sequenced high-depth transcriptomes from the workers and soldiers of a lower termite, Neotermes binovatus (Kalotermitidae), to investigate the differentially expressed termite transcripts, flagellate transcript abundance, and co-expression patterns of the host–flagellate transcript pairs in both castes. The worker-biased transcripts were enriched in functions related to cuticle development, nervous system regulation, pheromone biosynthesis, and metabolism, whereas the soldier-biased transcripts were predominantly involved in muscle development and kinesis, body morphogenesis, protein modification, and aggression. Flagellate transcripts from the orders Cristamonadida, Trichomonadida, Tritrichomonadida, and Oxymonadida were identified in both workers and soldiers, with the abundance of most flagellate transcripts tending to be higher in workers than in soldiers. Furthermore, we observed a much larger number of strong co-expression correlations between the termite and flagellate transcripts in workers than in soldiers, suggesting the possibility that soldiers depend more on food processed by worker holobionts than on their own symbiotic system. This research provides insights into the functional specialization of the host–flagellate symbiotic system in the worker and soldier castes of termites, supporting the workers’ roles in nest maintenance, preliminary food processing, and communication, while emphasizing the defensive role of soldiers. Additionally, it offers new perspectives on the potential termite-flagellate interactions and underscores the need for whole-genome data of termite flagellates in further studies. Full article

Ants as social insects live in groups, which increases the risk of contagious diseases. In response to the threat of pathogens, ants have evolved a variety of defense mechanisms, including incorporating antimicrobial chemicals into nest material for nest hygiene. Crematogaster rogenhoferi is an arboreal ant, building its nest using plant tissues. It is still unclear how C. rogenhoferi is protected against pathogens in its nest. Two main chemicals, 2,2′-methylenebis[6-(1,1-dimethylethyl)-4-methyl-phenol] (MP) and lup-20(29)-en-3-one (LP), isolated from nest materials of C. rogenhoferi were used to investigate ants’ anti-pathogenic activity against the entomopathogenic fungus Beauveria bassiana and the entomopathogenic bacteria Serratia marcescens. The results showed that MP and LP can inhibit the growth of B. bassiana through direct contact and fumigation. However, neither MP nor LP had any negative effect on S. marcescens growth. Subsequent analysis showed that MP was found in both the abdomen part and the head part of C. rogenhoferi workers, and LP was not detected in C. rogenhoferi workers. Since LP is a common plant secondary metabolite, it is implied that LP may originate from the plant tissue of C. rogenhoferi nest materials. Our results showed that C. rogenhoferi capitalizes on its own antimicrobial chemicals and probably the chemical defenses which have evolved in plants to protect itself against pathogens. Full article

Foliar blast, caused by Pyricularia grisea, poses a major challenge to pearl millet (Pennisetum glaucum (L.) R. Br) production, leading to severe yield losses, particularly in rainfed ecologies. This study aimed to elucidate the genetic basis of blast resistance through a genome-wide association study (GWAS) involving 281 diverse pearl millet inbreds. GWAS panel was phenotyped for blast resistance against three distinct isolates of P. grisea collected from Delhi, Gujarat, and Rajasthan locations, revealing a significant variability with 16.7% of the inbreds showing high resistance. Bayesian information and linkage disequilibrium iteratively nested keyway (BLINK) and Multi-Locus Mixed Model (MLMM) models using transformed means identified 68 significant SNPs linked to resistance, with hotspots for resistance-related genes on chromosomes 1, 2, and 6. These regions harbor genes involved in defense mechanisms, including immune response, stress tolerance, signal transduction, transcription regulation, and pathogen defense. Genes, namely 14-3-3-like proteins RGA2, RGA4, hypersensitive-induced response proteins, NHL3, NBS-LRR, LRR-RLK, LRRNT_2, and various transcription factors such as AP2/ERF and WRKY, played a crucial role in the stress-responsive pathways. Analyses of transporter proteins, redox processes, and structural proteins revealed additional mechanisms contributing to blast resistance. This study offers valuable insights into the complex genetic architecture of blast resistance in pearl millet, offering a solid foundation for marker-assisted breeding programs and gene-editing experiments. Full article

The integumentary system of the leatherback sea turtle (Dermochelys coriacea) is the most visible and defining difference of the species, with its smooth and waxy carapace and finely scaled skin, distinguishing it from the other six sea turtle species. The skin is the body’s largest organ and serves as a primary defense against the outside world and is thus essential to health. To date, we have begun to understand that the microorganisms located on the skin aid in these functions. However, many host–microbial interactions are not yet fully defined or understood. Prior to uncovering these crucial host–microbial interactions, we must first understand the communities of microorganisms present and how they differ through life-stage classes and across the body. Here, we present a comprehensive bacterial microbial profile on the skin of leatherbacks. Using next-generation sequencing (NGS), we identified the major groups of bacteria on the skin of neonates at emergence, neonates at 3–4 weeks of age (i.e., post-hatchlings), and nesting females. These data show that the predominant bacteria on the skin of the leatherback are different at each life-stage class sampled. This suggests that there is a shift in the microbial communities of the skin associated with life-stage class or even possibly age. We also found that different sample locations on the nesting female (i.e., carapace and front appendages = flipper) have significantly different communities of bacteria present. This is likely due to differences in the microhabitats of these anatomic locations and future studies should explore if this variation also holds true for neonates. These data define baseline skin microbiota on the leatherback and can serve as a foundation for additional work to broaden our understanding of the leatherbacks’ host–microbial interactions, the impacts of environmental changes or stressors over time, and even the pathogenicity of disease processes. Full article

Queen triggerfish Balistes vetula is an important reef-associated species for commercial fisheries in the U.S. Caribbean. It exhibits a relatively unique reproductive strategy as a nesting benthic spawner, investing substantial energy in territorial defense, building and maintaining nests, and caring for fertilized eggs during the reproductive season. Prior to this study, no comprehensive life history information existed in the literature for queen triggerfish. This study provides the first comprehensive documentation of age, growth, size/age at sexual maturity, reproductive seasonality, and reproductive output for a Balistes species in the Caribbean. From 2013 to 2023, we collected 2190 fish samples from fisheries-dependent and -independent sources from the waters of Puerto Rico and the U.S. Virgin Islands. Fish ranged from 67 to 477 mm fork length (FL). We documented that queen triggerfish is sexually dimorphic with males attaining larger mean sizes-at-age compared to females and the species is characterized by a moderately young age at median sexual maturity (A₅₀ = 3.3 y). The maximum age for our U.S. Caribbean samples was 23 y based on increment counts from otoliths. Spawning season encompassed the months of December to August in the region, and female spawning frequency ranged from an estimated 2 to 84 times per year; female spawning frequency increased with increasing size and age of fish. We documented that commercial fishers in the U.S. Caribbean mainly target “plate-size” individuals, defined in our study as 235–405 mm FL, which appears to act as a self-imposed slot size range limit and results in the fishery not removing individuals in the smallest and largest size groups at high rates. The percentage of immature fish from fisheries-dependent sources was close to 0 (0.8%). Commercial fishing for queen triggerfish in the region currently appears to be sustainable, but monitoring of the population should continue. Full article

We describe efforts to reintroduce the extinct-in-the-wild Spix’s Macaw (Cyanopsitta spixii) within its historical range in eastern Brazil. Twenty captive-reared Spix’s Macaws were released, along with 15 Blue-Winged Macaws (Primolius maracana), as heterospecific flocks in two events during the dry and rainy seasons of 2022. We monitored the release groups through daily observation and telemetry tracking from early June 2022 to early June 2023. We documented an overall first-year survival of 58.3% (CI: 37.8–78.7%), with 65% of Spix’s Macaws establishing a stable area of activity within 5 km of the release site, excluding any temporary long-distance forays. Eighty-five percent of released Spix’s Macaws exhibited flock cohesion, including interactions with and integration into wild Blue-Winged Macaw groups at the release site. Several released Spix’s Macaws formed pair-bonds with conspecifics and engaged in nest cavity exploration, breeding behavior, and territorial defense of nest sites, with three females also laying and incubating eggs. One nesting pair successfully hatched and reared chicks in an artificial nest cavity. These releases employed a novel reintroduction strategy using a surrogate species model, the sympatric Blue-Winged Macaw, to “mentor” and facilitate post-release adaptation by the target species and increase the total number of individuals in the release cohort. Lastly, participatory monitoring by local citizens is considered a way to engage and involve local communities in species and habitat conservation and potentially create new employment opportunities in the region. Full article

Age-related changes in behavior and sensory perception have been observed in a wide variety of animal species. In ants and other eusocial insects, workers often progress through an ordered sequence of olfactory-driven behavioral tasks. Notably, these behaviors are plastic, and workers adapt and rapidly switch tasks in response to changing environmental conditions. In the Florida carpenter ant, smaller minors typically perform most of the work needed to maintain the colony, while the larger majors are specialized for nest defense and rarely engage in these routine tasks. Here, we investigate the effects of age and task group on olfactory responses to a series of odorant blends in minor and major worker castes. Consistent with their respective roles within the colony, we observed significant age-associated shifts in the olfactory responses of minors as they transitioned between behavioral states, whereas the responses of majors remained consistently low regardless of age. Furthermore, we have identified a unitary compound, 3-methylindole, which elicited significantly higher responses and behavioral aversion in minor nurses than in similarly aged foragers suggesting that this compound may play an important role in brood care. Taken together, our results suggest that age- and task-associated shifts in olfactory physiology may play a critical role in the social organization of ant colonies. Full article

Unmanned Aerial Vehicles (UAVs) are increasingly utilized for urban patrol and defense owing to their low cost, high mobility, and rapid deployment. This paper proposes a multi-UAV mission planning model that takes into account mission execution rates, flight energy consumption costs, and impact costs. A kinematics and dynamics model of a quadcopter UAV is established, and the UAV’s flight state is analyzed. Due to the difficulties in addressing 3D UAV kinematic constraints and poor uniformity using traditional optimization algorithms, a lightning search algorithm (LSA) based on multi-layer nesting and random walk strategies (MNRW-LSA) is proposed. The convergence performance of the MNRW-LSA algorithm is demonstrated by comparing it with several other algorithms, such as the Golden Jackal Optimization (GJO), Hunter–Prey Optimization (HPO), Pelican Optimization Algorithm (POA), Reptile Search Algorithm (RSA), and the Golden Eagle Optimization (GEO) using optimization test functions, Friedman and Nemenyi tests. Additionally, a greedy strategy is added to the Rapidly-Exploring Random Tree (RRT) algorithm to initialize the trajectories for simulation experiments using a 3D city model. The results indicate that the proposed algorithm can enhance global convergence and robustness, shorten convergence time, improve UAV execution coverage, and reduce energy consumption. Compared with other algorithms, such as Particle Swarm Optimization (PSO), Simulated Annealing (SA), and LSA, the proposed method has greater advantages in addressing multi-UAV trajectory planning problems. Full article