Search Results (5,896)

We report the first synthesis of IspH-directed prodrugs targeting the terminal enzyme of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (IspH or LytB). A series of alkyne and pyridine monophosphate cycloSaligenyl (cycloSal) prodrugs were prepared to enhance membrane permeability by masking the phosphate group. The effects of electron-withdrawing (Cl, CF₃) and electron-donating (OCH₃, NH₂) substituents were examined, together with amino acid-functionalized and mutual prodrug analogs. Among the synthesized compounds, chlorine-substituted derivatives 5c and 6c displayed the strongest antimycobacterial activity against Mycobacterium smegmatis, surpassing isoniazid in agar diffusion assays. These results indicate that electron-withdrawing substituents accelerate prodrug hydrolysis and facilitate intracellular release of the active inhibitor. This work provides the first experimental evidence of an IspH-targeted prodrug approach, highlighting the cycloSal strategy as a valuable tool for delivering phosphorylated inhibitors and developing novel antimycobacterial agents acting through the MEP pathway. Full article

Safflower (Carthamus tinctorius L.) is increasingly attracting the attention of Mediterranean farmers due to its broad environmental adaptability and low input requirements. Although still relatively underexplored, this species holds remarkable potential as a source of natural dyes and bioactive phytochemicals with recognized health-promoting and phytotherapeutic properties. In this study, the effects of genotype and sowing time on safflower’s productive and qualitative traits were investigated by testing six genotypes and two sowing times (autumn and spring) in an open-field trial conducted in central Tuscany. The Pieve genotype achieved the highest floret dry yield per head, number of heads per plant, and total floret yield per plant, whereas the Montola 2000 genotype was distinguished by its elevated polyphenol concentration and pronounced antioxidant activity. Autumn sowing resulted in higher yields of bioactive pigments, including carthamin and yellow quinochalcones, alongside greater total phenolic content and antioxidant capacity. Conversely, spring sowing appeared to limit pigment biosynthesis, likely due to environmental stressors such as elevated temperature and excessive light exposure. Overall, these findings highlight the strong influence of genotype and sowing time on the accumulation of health-beneficial compounds in safflower. By optimizing these factors, safflower can be strategically valorized as a multipurpose crop in the Mediterranean region, combining economic and environmental sustainability with the production of natural compounds of high nutraceutical and phytotherapeutic value. Full article

This paper presents the results of a comprehensive technological study of three bronze sculptures from the Jin Empire period (1115–1234 AD) from the collection of the Museum of Archaeology and Ethnography at the Institute of History, Archaeology and Ethnography of the Peoples of the Far East, Far Eastern Branch of the Russian Academy of Sciences (IHAE FEB RAS). Using photon-counting computed tomography (PCCT) and energy-dispersive X-ray spectroscopy (EDS), the production techniques were reconstructed, differences in alloy composition were identified, and specific features of the casting processes were determined. Tomographic analysis revealed two fundamentally different manufacturing approaches: a multi-stage technology involving the use of different alloys and the assembly of separately cast elements, and a single-cast technology with a homogeneous structure. Elemental analysis of the three sculptures using EDS demonstrated significant compositional variability—from 21% to 67% copper and from 9% to 69% tin in different parts of the objects—confirming the complexity of the technological processes. An expanded study of 20 bronze sculptures using portable X-ray fluorescence analysis (pXRF) allowed for the identification of four typological alloy groups: classic balanced lead–tin bronzes (Cu 30–58%, Sn 16–23%, Pb 16–28%), high-lead bronzes (Pb up to 52%), high-tin bronzes (Sn up to 30%), and low-tin alloys (Sn less than 11%). The morphological features of the sculptures suggest one of their possible interpretations as ancestor spirits used in ritual practices. The research findings contribute to the study of Jurchen metallurgical traditions and demonstrate the potential of interdisciplinary, non-destructive analytical methods for reconstructing the technological, social, and cultural aspects of medieval Far Eastern societies. Full article

Ten new species of Lonchaeidae in the genera Lonchaea Fallen and Silba Macquart are described, nine from the Australasia–Oceania realm—Papua New Guinea (7 species), Solomon Islands (1 species), and Sulawesi (1 species)—and one from Sumatra in the Indo-Malayan realm. The new species are Lonchaea bacchusi sp. nov., Lonchaea herzogi sp. nov., Lonchaea morobe sp. nov., Lonchaea spenceri sp. nov., Lonchaea sulawesi sp. nov., Silba guineai sp. nov., Silba honiara sp. nov., Silba ismayi sp. nov., Silba kokoda sp. nov., and Silba papua sp. nov. The male genitalia are illustrated, and the diagnostic features that distinguish these species are presented. The male genitalia of Lonchaea uniseta Malloch, 1930, from Samoa are described and illustrated for the first time, and the male genitalia of two species from Micronesia, Lonchaea belua McAlpine, 1964, and Lonchaea sabroski McAlpine, 1964, are illustrated for the first time. A checklist of the Lonchaeidae of Papua New Guinea is provided. Full article

In recent years, sialidases (neuraminidases) derived from non-clinical sources have attracted considerable interest due to their potential applications in the food and pharmaceutical industries. A deeper understanding of the mechanisms regulating sialidase synthesis could lead to more efficient enzyme production. Induction is considered a key regulatory mechanism. However, there is a lack of data on the regulation of sialidase synthesis in filamentous fungi. This study examines how regulatory mechanisms influence the production of a sialidase enzyme exhibiting high activity at low temperatures in the Antarctic fungal strain Penicillium griseofulvum P29. The inclusion of high- and low-molecular-weight substances possessing terminal non-reducing N-acetylneuramyl groups in the tests led to a marked enhancement of sialidase activity. The strongest induction response was elicited by sialic acid, followed by glycomacropeptide, milk whey, N-acetylglucosamine, N-acetylmannosamine, and colominic acid. RT-qPCR experiments demonstrated that induction occurs at the transcriptional level of the sialidase gene. Biochemical analysis elucidates the function of inducers as triggers in the de novo synthesis of the enzyme protein. To our knowledge, this is the first study to highlight the importance of regulatory mechanism induction in the synthesis of cold-active sialidases. Full article

After nearly two decades of developments in Historic/Heritage Building Information Modeling (HBIM), the field has reached a stage of maturity that calls for a critical reassessment of its evolution, achievements, and remaining challenges. Digital representation has become a central component of contemporary heritage conservation, enabling advanced methods for analysis, management, and communication. This review examines the maturation of HBIM as a comprehensive framework that integrates extended reality (XR), artificial intelligence (AI), machine learning (ML), semantic segmentation and Digital Twin (DT). Six major research domains that have shaped recent progress are outlined: (1) the application of HBIM to restoration and conservation workflows; (2) the expansion of public engagement through XR, virtual museums, and serious games; (3) the stratigraphic documentation of building archaeology, historical phases, and material decay; (4) data-exchange mechanisms and interoperability with open formats and Common Data Environments (CDEs); (5) strategies for modeling geometric and semantic complexity using traditional, applied, and AI-driven approaches; and (6) the emergence of heritage DT as dynamic, semantically enriched systems integrating real-time and lifecycle data. A comparative assessment of international case studies and bibliometric trends (2015–2025) illustrates how HBIM is transforming proactive and data-informed conservation practice. The review concludes by identifying persistent gaps and outlining strategic directions for the next phase of research and implementation. Full article

Black crust and spalling are common deterioration phenomena affecting marble relics, yet their correlation remains inadequately understood. Hyperspectral imaging, reflectance spectroscopy, portable X-ray Fluorescence (p-XRF), infrared thermography, Scanning Electron Microscopy coupled with Energy-Dispersive Spectroscopy (SEM-EDS), and microbiological analysis was employed to connect these two types of deterioration on the Danbi stone carving of the Confucian Temple in Beijing. Spectral and thermal analyses reveal that black crust significantly reduces reflectance and increase solar absorption by 27%, resulting in thermal stress. p-XRF and SEM-EDS analyses indicated that black crust is enriched in Fe, Ti, Zn, Pb, As and clay minerals, while spalling areas display increase Ca, reflecting substrate exposure. Microscopy reveals microcracks at the layer–substrate interface. Microbiological analyses identify Cladosporium anthropophilum and Alternaria alternata as contributors to surface-darkening. These multi-scale datasets collectively demonstrate that alterations in surface chemistry and bio-mediated darkening promoting the formation of black crusts, which subsequently induce marble spalling due to solar absorption and thermal stress. These findings clarify the coupled physical–chemical–biological pathways through which black crust accelerates stone spalling. Full article

Silver nanoparticles (AgNPs) are increasingly applied in agriculture and related technologies due to their antimicrobial properties, yet their interactions with soil-associated organisms and microbial communities remain insufficiently characterized. This study examined the effects of AgNP exposure (10.85 mg/L) on trace element accumulation and gut bacterial communities of the earthworm Eisenia fetida under two substrate conditions (horticultural substrate and compost). High-throughput 16S rRNA gene sequencing revealed substrate-dependent shifts in microbial community structure following AgNP exposure. Several bacterial taxa, including Proteobacteria, Gammaproteobacteria, Bacilli, Streptococcus sp., and Staphylococcus sp., exhibited pronounced numerical declines, indicating sensitivity to AgNPs, whereas Actinobacteria and Bacteroidetes showed comparatively higher relative abundances, suggesting greater tolerance. Compost partially mitigated the inhibitory effects of AgNPs on gut microbiota. Concurrently, AgNP exposure altered trace element accumulation patterns in earthworm tissues, highlighting interactions between silver uptake and elemental homeostasis. Collectively, these findings demonstrate that AgNPs can induce taxon- and substrate-specific responses in earthworm-associated microbial communities and metal accumulation, providing insight into potential ecological consequences of nanoparticle use in agricultural systems. Full article

Artificial Intelligence (AI) is increasingly reshaping how cultural heritage institutions design and deliver digital visitor experiences, particularly through adaptive Augmented Reality (AR) applications. However, most existing AR deployments in museums and galleries remain static, rule-based, and insufficiently responsive to visitors’ contextual, behavioral, and emotional diversity. This paper presents ARIA (Augmented Reality for Interpreting Artefacts), a conceptual and architectural framework for AI-supported, adaptive AR experiences in cultural heritage settings. ARIA is designed to address current limitations in personalization, affect-awareness, and ethical governance by integrating multimodal context sensing, lightweight affect recognition, and AI-driven content personalization within a unified system architecture. The framework combines Retrieval-Augmented Generation (RAG) for controlled, knowledge-grounded narrative adaptation, continuous user modeling, and interoperable Digital Asset Management (DAM), while embedding Human-Centered Design (HCD) and Fairness, Accountability, Transparency, and Ethics (FATE) principles at its core. Emphasis is placed on accountable personalization, privacy-preserving data handling, and curatorial oversight of narrative variation. ARIA is positioned as a design-oriented contribution rather than a fully implemented system. Its architecture, data flows, and adaptive logic are articulated through representative museum use-case scenarios and a structured formative validation process including expert walkthrough evaluation and feasibility analysis, providing a foundation for future prototyping and empirical evaluation. The framework aims to support the development of scalable, ethically grounded, and emotionally responsive AR experiences for next-generation digital museology. Full article

Globally, ecomuseums, as examples of integrated museums, have prioritised local community participation as well as the promotion of cultural heritage and territoriality in the field of ecotourism since the 1970s. However, in Ghana, ecomuseums are a less explored extended activity at ecotourism sites. Preliminary research at the Bomfobiri Wildlife Sanctuary in the Ashanti Region of Ghana revealed that there is less patronage of the ecotourism facility due to the absence of extended activities targeted at visitor engagement and experience. This convergent parallel mixed methods study rooted in phenomenology and descriptive survey design investigated the possibility of establishing an ecomuseum at the sanctuary. The study found that the establishment of an ecomuseum facility would promote Kumawu’s cultural heritage. Also, it would enhance visitor engagement, promote local community ownership, increase revenue generation, and lead to the conservation of nature. The study contends that the establishment of an ecomuseum would lead to greater patronage of Ghana’s Protected Areas while creating job avenues, especially for forest-fringe communities. The study proposes an investment into the establishment of locally specific extended activities in the various nature reserves, zoological parks, and gardens, as well as wildlife sanctuaries in the country, to enhance ecotourism development in Ghana. Full article

Due to the unpredictability of seismic and the complexity of collection environments, significant uncertainty exists regarding their impact on cultural relics. Moreover, existing research on the causal analysis of seismic damage to cultural relics remains insufficient, thereby limiting advancements in risk assessment and protective measures. To address this issue, this paper proposes a seismic damage risk assessment method for cultural relics in collections, integrating deep learning and reinforcement strategies. The proposed method enhances the dataset on seismic impacts on cultural relics by developing an integrated deep learning-based data correction model. Furthermore, it incorporates a graph attention mechanism to precisely quantify the influence of various attribute factors on cultural relic damage. Additionally, by combining reinforcement learning with the Deep Deterministic Policy Gradient (DDPG) strategy, this method refines seismic risk assessments and formulates more targeted preventive protection measures for cultural relics in collections. This study evaluates the proposed method using three public datasets in comparison with the self-constructed Seismic Damage Dataset of Cultural Relics (CR-SDD). Experiments are conducted to assess and analyze the predictive performance of various models. Experimental results demonstrate that the proposed method achieves an accuracy of 81.21% in assessing seismic damage to cultural relics in collections. This research provides a scientific foundation and practical guidance for the protection of cultural relics, offering strong support for preventive conservation efforts in seismic risk mitigation. Full article

This article presents a comprehensive analysis of the polychrome paintings on the Flower Peking Opera Theatre in Bozhou, Anhui Province, China. A multi-technique approach was employed, including polarized light microscopy (PLM), X-ray fluorescence (XRF), micro-Raman spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy with energy-dispersive spectrometry (SEM-EDS), and Herzberg staining to determine the composition and methodologies involved in the formation of the pigment layer, the white primer, and the ground layer. The analysis identified cinnabar (red), both artificial ultramarine and Prussian blue (blue), a mixture of barite and gypsum (white), a mixture of chromite and Prussian blue (green), and carbon black (black) in the pigment layer. The ground layer was found to consist of clay and plant fibers (cotton and hemp), while the white prime layer was primarily composed of barite and gypsum. This research provides insights for future conservation and restoration efforts. Full article

Fecal retention is a distinctive reproductive strategy in certain leaf beetles, which enables females to use accumulated fecal material to protect their eggs and enhance offspring survival. The adult flea beetle Asiophrida xanthospilota (Baly, 1881) is a specialist herbivore that feeds on the leaves of Cotinus coggygria Scop. (Anacardiaceae). Using light microscopy, scanning electron microscopy, and micro-computed tomography, we described and illustrated the hindgut anatomy of adult female A. xanthospilota during the pre-mated and post-mated reproductive phases. We further examined the physiological changes in the hindgut associated with fecal retention, and assessed hindgut muscle activity across these two reproductive stages. The hindgut of adult A. xanthospilota consists of three regions: ileum, colon, and rectum. The ileum is a thin, straight or coiled, tube enclosed by malpighian tubules and supported by circular and longitudinal muscles. The colon lies between the ileum and rectum, possesses a chitinized cuticle, and is externally covered with tracheae and tracheoles. A rectal valve separates the colon from the rectum, which forms the posterior end of the alimentary canal and is characterized by intimal spines and robust circular muscles. During the post-mated phase, fecal retention causes pronounced dilation of the hindgut, substantially increasing the volume occupied by food remnants. Electromyographic recordings revealed high hindgut muscle activity in pre-mated females, characterized by short and variable bursts, whereas post-mated females exhibited reduced activity with longer and more sustained bursts. The functional implications of these specialized structural features are discussed. Overall, these morphological and physiological adaptations enhance the fecal retention strategy by increasing fecal capacity, regulating hindgut motility, and enabling the formation of a protective fecal case around the egg mass. Full article

Active involvement in creative activities, known as creative health, has been shown to enhance wellbeing, with museums serving as unique spaces for health promotion; however, visitors often require guidance to derive significant benefits from these institutions. This study, part of the larger ASBA (Anxiety, Stress, Brain-friendly museum Approach) project, evaluates the first phase of an intervention specifically focused on a Mindfulness protocol adapted to museum contexts. It has employed a single-group pre–post design with 79 healthy adults recruited from the non-clinical population. Participants were involved in a 15 min standardized mindfulness practice adapted from Mindfulness-Based Stress Reduction (MBSR) in either an art or science museum. State anxiety (SAI) and mood (VAS) were assessed at baseline and post-intervention, alongside personality traits (BFI-10) and interest measures to identify individual moderators of treatment response. The practice appeared to reduce state anxiety significantly in both settings, with large effect sizes. Specific moderators emerged: openness to experience predicted anxiety reduction in the art museum, whereas science interest predicted outcomes in the science setting. These findings suggest that brief, standardized mindfulness protocols implemented through the ASBA framework can provide promising immediate benefits for visitor wellbeing across diverse museum environments. Full article