Search Results (249)

The aim of translucency-enhancing liquids (TEL) is to locally influence the phase composition of zirconia in order to increase its translucency. This study aimed to determine the influence of TEL on 3Y- and 4Y-TZP zirconia concerning roughness, hardness, wear, flexural strength, dynamic stability and fracture force of fixed dental prostheses after thermal cycling and mechanical loading. Two zirconia materials (4Y-TZP; 3Y-TZP-LA, n = 8 per material and test) were investigated with and without prior application of TEL. Two-body wear tests were performed in a pneumatic pin-on-block design (50 N, 120,000 cycles, 1.6 Hz) with steatite balls (r = 1.5 mm) as antagonists. Mean and maximum vertical loss as well as roughness (Ra, Rz) were measured with a 3D laser-scanning microscope (KJ 3D, Keyence, J). Antagonist wear was determined as percent area of the projected antagonist area. Martens hardness (HM; ISO 14577-1) and biaxial flexural strength (BFS; ISO 6872) were investigated. The flexural fatigue limit BFSdyn was determined under cyclic loading in a staircase approach with a piston-on-three-ball-test. Thermal cycling and mechanical loading (TCML: 2 × 3000 × 5 °C/55 °C, 2 min/cycle, H₂O dist., 1.2 × 10⁶ force á 50 N) was performed on four-unit fixed dental prostheses (FDPs) (n = 8 per group) and the fracture force after TCML was determined. Statistics: ANOVA, Bonferroni test, Kaplan–Meier survival, Pearson correlation; α = 0.05. TEL application significantly influences roughness, hardness, biaxial flexural strength, dynamic performance, as well as fracture force after TCML in 3Y-TZP. For 4Y-TZP, a distinct influence of TEL was only identified for BFS. The application of TEL on 3Y- or 4Y-TZP did not affect wear. TEL application has a strong effect on the mechanical properties of 3Y-TZP and minor effects on 4Y-TZP. All effects of the TEL application are of a magnitude that is unlikely to restrict clinical application. Full article

Increasing temperatures associated with climate change will lead to (periodic) temperature-induced reductions in food intake in human and other mammal populations. Human adults, however, are both tolerant and resilient to periodic nutritional deficits, and the associated health effects should be limited. Intermittent nutritional deficits may also cause growth restriction in developing foetuses and young children, which potentially affects their food intake in later life. Therefore, temperature-induced hypophagia can be hypothesised to manifest as later compensatory responses with multiple concomitant (or extended) lags of varying temporal dimensions. We examined the relationship between calorie intake and ambient outdoor temperatures for a time series covering past decades (FAO data for 1961–2013) in 80 countries to determine if humans alter their food intake in response to elevated temperatures. We included eleven different temporal “windows of exposure” of varying lag. These windows considered current and recent exposure, just as lagged effects allowed for a consideration of past effects on mothers, their children, and childhood exposure. It was hypothesised that one of these could provide a basis for predicting future changes in human calorie intake in response to climate change. Our analyses showed no apparent association with temperatures in ten of the eleven hypotheses/models. The remaining hypothesis suggests that current calorie intake is linked to decadal mean temperatures with a lag of approximately three decades, pointing to an impact on mothers and their (developing) children. The impact of an increase in mean temperature varies with temperature amplitudes, and negative impacts are only found in countries with low temperature amplitudes (warmer countries), albeit the impact on calorie intake caused by a 2–3 °C change in temperatures or temperature amplitudes is generally modest. However, in considering calorie intake, we only address quantities of food (with unspecified quality), which insufficiently reflect the full range of nutritional challenges associated with increasing temperatures. Understanding climate-driven changes in human food intake requires global interdisciplinary collaboration across public health, environmental science, and policy. Full article

Background: Several studies document the importance of communicative abilities for children’s development. Especially in recent years verbal communication in preschool children with developmental language disorder (DLD) has been studied, relying heavily on statistical analysis, outcome measures, or/and parents’ reports. Purpose: This explorative study investigates the effects of speech therapy on the development of language and verbal communication skills in preschool children with DLD within their peer group in a day-to-day setting using objective video-documentation. Hypothesis: Speech therapy leads to improvement of language, communication, and possibly to concurrent development of both language and verbal communication skills in preschool children. Methods: Preliminary prospective study to assess language and verbal communications skills of nine preschool children (seven boys, two girls, 4–6 y) with DLD in a speech therapy kindergarten using video recordings over a one-year therapy period. The communicative participation of the members of the peer group was assessed and included the verbal address (Av) and the ratio of “verbal address/verbal reaction” (Av/Rv). Results: The investigation results in evidence for two outcome groups: One group with suspected preferential verbal communication disorders (n = 4) was characterised by a high Av/Rv value, meaning they were scored to have a normal or high verbal address (Av) and a low verbal response (Rv) (predominantly interpersonal communication related disorder). This group showed minimal changes in the short term but demonstrated improvement after 5 years of schooling; thus, pedagogical activities seemed to be particularly effective for these children. The second group showed a balanced Av/Rv ratio (predominantly language related disorder) (n = 5); but after five years they demonstrated a partial need for special school support measures. This group may therefore particularly benefit from speech therapy. Conclusions: The present study clearly shows that even with speech-language therapy, the linguistic ability of DLD-disturbed children does not necessarily develop simultaneously with their communication ability. Rather, the investigations provide evidence for two groups of preschool children with DLD and communication disorder: One group demonstrated a predominantly verbal communication related disorder, where pedagogical intervention might be the more important treatment. The second group showed predominantly DLD, therefore making speech therapy the more effective intervention. In this study, all children expressed their desire to communicate with their peers. To the authors’ best knowledge, this is the first study determining the ability to communicate in a preschool cohort with DLD using characterisation with video documentation in a follow-up for 1 year. Full article

Background/Objectives: This study investigates the macular microvasculature in a large cohort of primary Sjögren’s disease (SjD) patients using optical coherence tomography angiography (OCTA), focusing on how disease duration, activity, and hydroxychloroquine (HCQ) treatment influence retinal microcirculation. Methods: A total of 106 eyes (53 SjD patients) and 70 eyes (35 age- and gender-matched healthy controls (HCs)) were examined. The vessel area density (VAD, %) and foveal avascular zone (FAZ, mm2) were measured in three retinal layers: Superficial Vascular Plexus (SVP), Intermediate Capillary Plexus (ICP), and Deep Capillary Plexus (DCP), respectively, in three peri-macular circular sectors (c1, c2, c3) each. Results: The VAD was significantly lower in c1 of the DCP in SjD compared to HCs (29.14 ± 7.07 vs. 31.78 ± 9.55, p = 0.038). The FAZ was significantly larger in SjD in both SVP (0.41 ± 0.13 vs. 0.34, 0.11, p < 0.001; Cohen’s |d| = 0.55) and DCP (0.45 ± 0.15 vs. 0.4 ± 0.14, p = 0.014; Cohen’s |d| ± 0.38). Significant correlations were observed between the FAZ size and reductions in the VAD in the SVP and DCP (p = 0.010, Cohen’s |d| = 0.2; p < 0.001, Cohen’s |d| ± 0.26) and across all layers combined (p = 0.019, Cohen’s |d| = −0.18). Conclusions: There was a negative correlation between the VAD in the DCP and disease duration (ρ = −0.28, p = 0.040). No significant correlation was identified between the duration of HCQ intake and the VAD or FAZ. Our findings indicate microvascular alterations in the DCP of SjD, characterized by a reduced VAD and an enlarged FAZ, which may be attributable to inflammatory or arteriosclerotic factors. OCTA may prove to be a valuable tool for the stratification of vascular risk in SjD. Full article

A series of incidents involving damage to vehicle speed sensor cables occurred in an urban area in Japan. At the request of the police, DNA analysis was conducted to identify the animal species responsible. Swab samples collected from the damaged sections of the cables were subjected to PCR testing using mtDNA fragments. Sequencing analysis with universal primers (SCPH02500, SCPL02981) detected DNA from the Japanese marten (Martes melampus). A comprehensive examination that included morphological analysis of the cable damage and consideration of the ecological characteristics of the Japanese martens suggested that the damage was likely caused by this species. DNA analysis using mtDNA markers is a valuable tool for species identification in wildlife forensic veterinary investigations and serves as important scientific evidence in criminal cases involving animals. The findings from this case may contribute to future investigations in forensic veterinary science and ecological research and may also inform measures to prevent human–wildlife conflicts involving animals. Full article

Currently, the need for resource efficiency and CO₂ reduction is growing in industrial production, particularly in the automotive sector. To address this, the industry is focusing on lightweight components that reduce weight without compromising mechanical properties, which are essential for passenger safety. Hybrid designs offer an effective solution by combining weight reduction with improved mechanical performance and functional integration. This study focuses on a one-step manufacturing process that integrates forming and bonding of hybrid systems using compression molding. This approach reduces production time and costs compared to traditional methods. Conventional Post-Mold Assembly (PMA) processes require two separate steps to combine fiber-reinforced plastic (FRP) structures with metal components. In contrast, the novel In-Mold Assembly (IMA) process developed in this study combines forming and bonding in a single step. In the IMA process, glass-mat-reinforced thermoplastic (GMT) is simultaneously formed and bonded between two metal belts during compression molding. The GMT core provides stiffening and load transmission between the metal belts, which handle tensile and compressive stresses. This method allows to produce hybrid structures with optimized material distribution for load-bearing and functional performance. The process was validated by producing a lightweight hybrid brake pedal. Demonstrating its potential for efficient and sustainable automotive production, the developed hybrid brake pedal achieved a 35% weight reduction compared to the steel reference while maintaining mechanical performance under quasi-static loading Full article

The genus Musa L. is one of the most important genera worldwide due to its use in food as a source of carbohydrates. A morphological characterization was performed to evaluate the potential of 100 accessions of Musa spp. from the Amazon region of Ecuador, applying 73 qualitative and quantitative descriptors in addition to the ecogeographic characterization. The multivariate analyses identified four large groups: The first is composed of the Musa AAB Simmonds ecotype “Hartón Plantain” and the “Cuerno Clone”. The second group is composed of the Musa acuminata Colla ecotype “Orito”. The third group is composed of the Musa acuminata ecotype “Malay plantain or red plantain”; and the fourth group is composed of the Musa × paradisiaca L. AAB ecotype “Barraganete” and banana or banana materials and the Musa AAB Simmonds ecotype “Plátano Dominico”. The qualitative descriptors with the highest discriminant value were the shape of the ♂ floret bud, the appearance of the rachis, and the pigmentation of the compound tepal, and the quantitative discriminant characters were the height of the pseudostem, the length of the leaf blade, the width of the leaf blade, and the weight of the raceme. The analysis with CAPFITOGEN of these 100 accessions through the ecogeographic characterization map identified 23 categories, highlighting category 20 with a coverage of 40.35%, which mainly includes the provinces of Orellana, Sucumbíos, part of Napo, Pastaza, and Morona Santiago. This category occurs within an annual temperature range between 21.6 °C and 27 °C, an apparent density of 1.25 to 1.44 g cm⁻³, and a cation exchange capacity (CEC) of 4 to 29 Cmol kg⁻¹. The morphological characterization of 100 Musa accessions revealed significant phenotypic variability, with four distinct morphological groups identified through cluster analysis. Key differences were observed in traits such as bunch weight, fruit length, and vegetative vigor. This variability highlights the potential of certain accessions for use in genetic improvement programs. The findings contribute valuable information for the efficient conservation, selection, and utilization of the Musa germplasm in Ecuadorian agroecosystems. The results demonstrate the existence of an important genetic variability in the INIAP Musa Germplasm Bank in the Ecuadorian Amazon region. Full article

The evolving landscape of Health Technology Assessment (HTA) in Europe, shaped by the implementation of the new EU HTA Regulation (HTAR), places an emphasis on engaging all stakeholders, including patients, in collaborative evidence generation. Yet integrating patients’ perspectives into critical processes like PICO scoping remains a challenge, with concerns around subjectivity, representativeness, and methodological robustness. This opinion paper examines the complexities of patient engagement in HTA, highlighting both the opportunities for patients to make meaningful contributions and the barriers that stand in the way. We propose a framework that employes the Delphi panel methodology to (1) foster scientific validity and increase transparency in patient contributions, (2) establish a structured and consistent patient engagement framework, and (3) and understand European patients’ perspectives while promoting collaboration among EU countries. By facilitating iterative feedback and fostering agreement among diverse groups of patients and caregivers contributing with their expertise, consensus methods like Delphi panels can help refine PICO criteria, align diverse stakeholders’ expectations, and increase the relevance of HTA outcomes. A study is now underway to evaluate the feasibility and value of using the modified Delphi panel methodology for patient engagement in PICO scoping. The authors propose that embracing patient engagement through carefully designed consensus frameworks could enhance the legitimacy and completeness of HTA processes, driving more patient-centered decision making across Europe. Full article

We report a landscape-scale natural experiment that followed the abundance and demography of forest-floor small mammals and the activity of small mustelids over a 4-year period of an extreme heat wave and abundant coniferous cone crops. Deer mice (Peromyscus maniculatus) and southern red-backed voles (Myodes gapperi) are major species in the coniferous forest-floor small mammal community near Summerland in southern British Columbia, Canada. Their major mammalian predators include the short-tailed weasel (Mustela richardsonii), long-tailed weasel (Neogale frenata), and American marten (Martes americana). We evaluated three hypotheses (H) that may explain the changes in these mammals from 2021 to 2024: (H₁) that large coniferous cone crops in 2022 would have generated high populations of forest-floor small mammals in 2023 owing to enhanced reproductive output and overwinter survival; (H₂) that increased activity of mustelids would have followed population increases, resulting in the decline of small mammal prey in 2024; and (H₃) that the widespread occurrence of cone crops in 2022 would also have elicited the same mammalian responses in 2023 at a second study area (Golden, BC) 276 km and three mountain ranges from Summerland. During the summer periods of each year, small mammal populations were monitored by intensive live-trapping, and mustelid presence was measured via an index of activity based on live traps, fecal scats, and predation events. The mean abundance and reproductive performance of the P. maniculatus and M. gapperi populations increased in response to the coniferous seedfall, thereby supporting H₁. The activity of small mustelids responded positively to increased numbers of small mammal prey and potentially acted in a regulatory and top–down function in these communities, and hence partially support H₂. Similar responses at Summerland and Golden indicated that this seedfall event and changes in the mammalian community occurred at a landscape-scale, thereby providing partial support for H₃. Potential differential effects of large seed crops on consumers did not affect the mean abundance patterns for P. maniculatus but apparently reduced this metric for M. gapperi. Heat waves, induced by anthropogenic climate change, may alter the frequency of coniferous masting events, and their effects may temporarily change the number and species of mammalian seed consumers and their predators. Full article

Human populations are scattered worldwide and live under widely different climates. Like other mammals, humans respond to climatic influences through various processes involving behavior, physiology, and various forms of adaptation. Human populations can be explored in investigating patterns of adaptation because many of their biological attributes have been monitored for over a century. Here, we evaluated the association between several thermal indices and human birth weight (BW) and offered some initial observations on the temporal integration of thermal cues associated with pregnancy outcomes. We compiled three datasets: (1) a dataset with global coverage of recent BWs; (2) an extended time series for seven European countries; and (3) a time series for four countries in equatorial Africa. Each dataset was analyzed for associations between BW and mean annual temperature, as well as seasonal and daily amplitudes. Mean annual temperatures, as well as seasonal and daily amplitudes, delivered consistent and comparable impacts in our analyses. The thermal indices can explain approx. 80% of the global variation in BW and 25–50% of the BW variation in time series covering the last 70 to 120 years. Mean BW in larger aggregates of humans (i.e., millions) is associated with several thermal indices, likely associated with systematic differences in proximate factors (e.g., maternal height, weight, food intake) between populations. This study underlines the diverse impact of the thermal environment on human reproduction, but it also underscores that this impact is less pronounced for differences in mean BW with respect to different communities, and it is possibly undetectable and/or irrelevant with respect to differences between individuals. Full article

Apicomplexan parasites of the genus Sarcocystis utilize a wide range of vertebrate hosts, including reptiles, birds, and mammals, and follow a complex two-host prey–predator life cycle. Sarcocysts develop within the muscle tissue of intermediate hosts, while oocyst sporulation occurs in the intestines of definitive hosts. Despite their broad host range, the role of Mustelidae predators in the transmission and maintenance of Sarcocystis species remains poorly understood, highlighting the need for targeted studies in this area. In 2023, 20 intestinal samples were collected from European pine martens (Martes martes) in Latvia. Light microscopy revealed sporocysts of Sarcocystis spp. in 70.0% of the samples. Sarcocystis species were identified by using the analysis of obtained cox1 and ITS1 sequences. Using molecular methods, Sarcocystis spp. were confirmed in 85.0% of the samples examined, and co-infections with two or more different parasite species were established in 30.0% samples. We detected eight Sarcocystis species (S. arieticanis, S. bertrami, S. capracanis, S. cruzi, S. entzerothi, S. hjorti, S. morae, and S. truncata) associated with intermediate hosts such as cervids, cattle, sheep, goats, and horses. Additionally, a genetically novel species, Sarcocystis sp. 25MmLV, was detected in a single European pine marten. These findings suggest that European pine martens serve as definitive hosts for multiple Sarcocystis species, particularly those infecting cervids. Full article

Recent advancements in nanomechanical microcantilever biosensors open new possibilities for clinical applications, permitting precise analysis of molecular interactions. The technology enables tracking gene expression, molecular conformational changes, antibody binding and antibiotic resistance. In particular, hybridization of DNA or RNA extracted from biopsies and whole blood from patients has led to significant advancements in diagnostics of critical medical conditions, e.g., cancer, bacteraemia and sepsis, utilizing rapid, sensitive, and label-free detection. Direct diagnosis from patient samples is a decisive advantage over competitive methods circumventing elaborate and time-consuming purification, amplification and cultivation procedures prior to analysis. Here, recent developments are presented from simple DNA hybridization of synthesized oligonucleotides to RNA material obtained from patients’ blood samples, highlighting technological advancements in diagnostic applications, such as detection of pathogens and disease biomarkers. We envisage our method to be a significant input to rapid, early and sensitive diagnosis directly from patients’ blood without requirements for amplification or cultivation. This would represent a paradigm shift in diagnostics, as no competing method currently exists. Full article

Tryptase is a tetrameric serine protease and a key component of mast cell granules. Here, we explored an integrated approach to characterize tryptase ligands, combining novel experimental binding studies using Surface Plasmon Resonance, with in silico analysis through the Exscalate platform. For this, we focused on three inhibitors previously reported in the literature, including a bivalent inhibitor and its corresponding monovalent compound. All three ligands showed concentration-dependent binding to immobilized human tryptase with the bivalent inhibitor showing the highest affinity. Furthermore, Rmax values were similar, indicating that the compounds occupy all four binding pockets of the tryptase tetramer. This hypothesis was supported by in silico computational analysis that revealed the binding mode of the monovalent ligand, one in each monomer pocket, compared with crystal structure of the bivalent one, which simultaneously occupies two binding pockets. Additionally, we solved the 2.06 Å X-ray crystal structures of human Tryptase beta-2 (hTPSB2), in both its apo form and in complex with compound #1, experimentally confirming the binding mode and the key molecular interactions predicted by docking studies for this compound. This integrated approach offers a robust framework for elucidating both the strength and mode of interaction of potential tryptase inhibitors. Full article

The pandemic threat from newly emerging viral diseases constitutes a major unsolved issue for global health. Antiviral therapy can play an important role in treating and preventing the spread of unprecedented viral infections. A repository of compounds exhibiting broad-spectrum antiviral activity against a series of different viral families would be an invaluable asset to be prepared for future pandemic threats. Utilizing an open innovation crowd-sourcing paradigm, we were able to identify a compound class of diphenylureas that exhibits in vitro antiviral activity against multiple viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), adenovirus, dengue virus, herpes, and influenza viruses. Compound 4 among the series exhibits strong activity against dengue virus, a growing global health problem with high medical need and no approved antiviral drug. The compounds are active against SARS-CoV-2 in a primary human stem cell-based mucociliary airway epithelium model and also active in vivo, as shown in a murine SARS-CoV-2 infection model. These results demonstrate the potential of the chemical class as antivirals on the one hand and the power of open innovation, crowd-sourcing, and repurposing on the other hand. Full article

Pancreatic islet transplantation (PIT) is a promising treatment for type 1 diabetes (T1D) but faces challenges pre- and post-transplantation. Co-transplantation with mesenchymal stromal cells (MSCs), known for their regenerative properties, has shown potential in improving PIT outcomes. This study examined the secretome of islets cultured alone compared to the secretomes of islets co-cultured with adipose-derived stromal cells (ASCs), a subtype of MSCs, under transplantation-relevant stressors: normoxia, cytokines, high glucose, hypoxia, and combined hypoxia and high glucose. Islet co-culture with ASCs significantly altered the proteome, affecting pathways related to energy metabolism, angiogenesis, extracellular matrix organization, and immune modulation. Key signaling molecules (e.g., VEGF, PDGF, bFGF, Collagen I alpha 1, IL-1α, and IL-10) were differentially regulated depending on culture conditions and ASC presence. Functional assays demonstrated that the co-culture secretome could enhance angiogenesis, collagen deposition, and immune modulation, depending on the stress conditions. These findings highlight possible mechanisms through which ASCs may support islet survival and function, offering insights into overcoming PIT challenges. Moreover, this work contributes to identifying biomarkers of the post-transplantation microenvironment, advancing therapeutic strategies for T1D and regenerative medicine. Full article