Microbial Biosensor: From Design to Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 1368

Special Issue Editor


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Guest Editor
Department of Liberal Arts, Tokyo University of Technology, Tokyo, Japan
Interests: microbial biosensors; yeast; BOD; toxicuty; soil; mediator
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The time has come for us human beings to recognize that we are no longer consumers of the Earth's ecosystem, but wasters. In order to live together with other living organisms on this planet, we need to change our lives. The Sustainable Development Goals (SDGs) were set and adopted as the “2030 Agenda” by the United Nations in 2015. Unfortunately, there are many difficulties in achieving that goal.

However, we must not give up on these goals; we must all do what we can, transcending national and regional boundaries. As Special Issue editors, although what we can do may be very small, we hope to go beyond barriers and contribute to the development of science and technology and the preservation of the global environment.

Microbial biosensors consisting of microbial cell(s) as an analyte sensor and a transducer as an electrical signal converter have been studied and developed for environmental, agricultural, food, and biomedical applications. These microbial biosensors have been supported by studying a variety of principles and device designs.

In the present Special Issue, we welcome submissions of research papers and critical reviews focusing on the following topics:

Design:

  • Novel designs of chip/cell/array for microbial biosensors;
  • Novel designs of self-powered device for microbial biosensors;
  • Novel designs of online, on-site, or remote monitoring; 
  • Novel designs for microbial immobilization;
  • Novel designs for single-microbial-cell biosensors.

Application:

  • Application to environmental water or wastewater monitoring; 
  • Application to estimate soil environment or bioremediation;
  • Application to agriculture, aquafarming, or aquaponics;
  • Micro/nanotechnology and novel materials applied to microbial biosensors;
  • Novel instrumentation systems for microbial biosensors.

Others:

  • New solutions applied in microbial biosensors;
  • Interdisciplinary study leading to microbial biosensor development.

Note: microbes generally refer to single-celled organisms, but in microbiology they also include viruses. In the present Special Issue, we are accepting a wide range of submissions regarding biosensors using these as sensing elements.

Dr. Hideaki Nakamura
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial biosensor
  • whole-cell biosensor
  • cell-based biosensor
  • microbial fuel cell
  • BOD
  • toxicity
  • immobilization
  • mediator
  • electron transfer
  • monitoring

Published Papers (1 paper)

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Research

13 pages, 2749 KiB  
Article
Real-Time On-Site Monitoring of Viruses in Wastewater Using Nanotrap® Particles and RICCA Technologies
by Vishnu Sharma, Hitomi Takamura, Manish Biyani and Ryo Honda
Biosensors 2024, 14(3), 115; https://doi.org/10.3390/bios14030115 - 21 Feb 2024
Viewed by 1107
Abstract
Wastewater-based epidemiology (WBE) is an effective and efficient tool for the early detection of infectious disease outbreaks in a community. However, currently available methods are laborious, costly, and time-consuming due to the low concentration of viruses and the presence of matrix chemicals in [...] Read more.
Wastewater-based epidemiology (WBE) is an effective and efficient tool for the early detection of infectious disease outbreaks in a community. However, currently available methods are laborious, costly, and time-consuming due to the low concentration of viruses and the presence of matrix chemicals in wastewater that may interfere with molecular analyses. In the present study, we designed a highly sensitive “Quick Poop (wastewater with fecal waste) Sensor” (termed, QPsor) using a joint approach of Nanotrap microbiome particles and RICCA (RNA Isothermal Co-Assisted and Coupled Amplification). Using QPsor, the WBE study showed a strong correlation with standard PEG concentrations and the qPCR technique. Using a closed format for a paper-based lateral flow assay, we were able to demonstrate the potential of our assay as a real-time, point-of-care test by detecting the heat-inactivated SARS-CoV-2 virus in wastewater at concentrations of 100 copies/mL and within one hour. As a proof-of-concept demonstration, we analyzed the presence of viral RNA of the SARS-CoV-2 virus and PMMoV in raw wastewater samples from wastewater treatment plants on-site and within 60 min. The results show that the QPsor method can be an effective tool for disease outbreak detection by combining an AI-enabled case detection model with real-time on-site viral RNA extraction and amplification, especially in the absence of intensive clinical laboratory facilities. The lab-free, lab-quality test capabilities of QPsor for viral prevalence and transmission in the community can contribute to the efficient management of pandemic situations. Full article
(This article belongs to the Special Issue Microbial Biosensor: From Design to Applications)
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