Search Results (293)

This study investigates the effect of pile diameter on the seismic performance of single piles using the kinematic interaction framework outlined in Method III of the Turkish Building Earthquake Code TBEC-2018. Pile diameters of 65 cm, 80 cm, and 100 cm were analyzed under four different soil profiles—soft clay, stiff clay, very loose sand-A, and very loose sand-B. The methodology integrated nonlinear spring modeling (P-y, T-z, Q-z) for soil behavior, one-dimensional site response analysis using DEEPSOIL, and structural analysis with SAP2000. The simulation results showed that increasing the pile diameter led to a significant rise in internal forces: the maximum bending moment increased up to 4.0 times, and the maximum shear force increased 4.5 times from the smallest to the largest pile diameter. Horizontal displacements remained nearly constant, whereas vertical displacements decreased by almost 50%, indicating improved pile–soil stiffness interaction. The depth of the maximum moment shifted according to the soil stiffness, and stress concentrations were observed at the interfaces of stratified layers. The findings underline the importance of considering pile geometry and soil layering in seismic design. This study provides quantitative insights into the trade-off between displacement control and force demand in seismic pile design, contributing to safer foundation strategies in earthquake-prone regions. Full article

Ultrafine fibers from poly(3-hydroxybutyrate) (PHB) and polyvinylpyrrolidone (PVP) and their blends with different component ratios in the range of 0/100 to 100/0 wt.% were obtained, and their structure and dynamic properties were studied. The polymers were obtained via electrospinning in solution mode. The structure, morphology, and segmental dynamic behavior of the fibers were determined using optical microscopy, SEM, EPR, DSC, and IR spectroscopy. The low-temperature maximum on the DSC endotherms provided information on the state of the PVP hydrogen bond network, which made it possible to determine the enthalpies of thermal destruction of these bonds. The PHB/PVP fiber blend ratio significantly affected the structural and dynamic parameters of the system. Thus, at low concentrations of PVP (up to 9%) in the structure of ultra-fine fibers, the distribution of this polymer occurs in the form of tiny particles, which are crystallization centers, which causes a significant increase in the degree of crystallinity (χ) activation energy (E_act) and slowing down of molecular dynamics (τ). At higher concentrations of PVP, loose interphase layers were formed in the system, which caused a decrease in these parameters. The strongest changes in the concentration of hydrogen bonds occurred when PVP was added to the composition from 17 to 50%, which was due to the formation of intermolecular hydrogen bonds both in PVP and during the interaction of PVP and PHB. The diffusion coefficient of water vapor in the studied systems (D) decreased as the concentration of glassy PVP in the composition increased. The concentration of the radical decreased with an increase in the proportion of PVP, which can be explained by the glassy state of this polymer at room temperature. A characteristic point of the 50/50% mixture component ratio was found in the region where an inversion transition of PHB from a dispersion material to a dispersed medium was assumed. The conducted studies made it possible for the first time to conduct a comprehensive analysis of the effect of the component ratio on the structural and dynamic characteristics of the PHB/PVP fibrous material at the molecular scale. Full article

The green octopus, Octopus hubbsorum, is a merobenthic species that inhabits warm-temperate waters in the eastern Pacific. However, its similarity to some morphological characteristics of and its slight genetic divergence from Octopus mimus has led to the proposal that both species are conspecific. The objective of this study was the morphological and molecular identification of eggs and paralarvae of the green octopus, O. hubbsorum, to provide information contributing to clarifying its taxonomy and relationship with O. mimus. The results obtained show that although O. hubbsorum has similarities with O. mimus in terms of egg size, chromatophore pattern, number of suckers, and presence of Kölliker’s organs, the O. hubbsorum paralarvae observed in this study are smaller (1.6 mm) and have a thin layer of loose skin, not described for O. mimus. Likewise, the morphology of the beak, radula, and suckers of O. hubbsorum is described for the first time and there are no studies of these structures for O. mimus. The phylogenetic analysis (mitochondrial cytochrome C oxidase subunit I and III genes) showed that both species form a monophyletic clade but belong to separate subclades. In conclusion, although the slight genetic divergence between these two species suggests conspecificity, their disjoint geographic distribution (O. hubbsorum is found in warm-temperate waters and O. mimus in cold-temperate waters) suggests the hypothesis of being two separate species with a close phylogenetic relationship. However, further research (morphological and population analyses) is required to solve taxonomic uncertainty. Full article

Red-layer soft rock has characteristics such as softening when encountering water, loose structure, and significant rheological properties. In tunnel engineering, it is necessary to sort out and analyze the stress characteristics of its support structure. This paper focuses on the mechanical behavior and support effect during the construction of Neogene red-layer soft rock tunnels. Through field monitoring, it explores the mechanical characteristics of Huizhou Tunnel under complex geological conditions in depth. This study adopted a remote wireless monitoring system to conduct real-time monitoring of key indicators including tunnel surrounding rock pressure, support structure stress, and deformation, obtaining a large amount of detailed data. An analysis revealed that the stress experienced by rock bolts is complex and varies widely, with stress values between 105 and 330.5 MPa. The peak axial force at a depth of 2.5 m reflects that the thickness of the loosened zone in the surrounding rock is approximately 2.5 m. The compressive stress in the steel arches of the primary support does not exceed 305.3 MPa. Shotcrete effectively controls the surrounding rock deformation, but the timing of support installation needs careful selection. The stress in the secondary lining is closely related to the primary support. The research findings provide an important theoretical basis and practical guidance for optimizing the support design of red-bed soft rock tunnels and enhancing construction safety and reliability. Full article

A lime–sand island–reef formation has a dual structure consisting of an overlying loose or weakly consolidated coral sand (CS) layer and an underlying reef limestone layer. The coral sand layer is the sole carrier of the underground freshwater lens in the lime–sand island–reef, and it differs in terms of its hydraulic properties from common terrigenous quartz sand (QS). This study investigated the mechanism of freshwater lens formation, dominated by solute dispersion, combining multi-scale experiments and numerical simulations (GMS) to reveal the control mechanisms behind the dispersion properties of coral sand and their role in freshwater lens formation. Firstly, the dispersion test and microscopic characterization revealed the key differences in coral sand in terms of its roundness, roughness, particle charge, and surface hydrophilicity. Accordingly, a hierarchical conversion model for the coral sand–quartz sand coefficient of dispersion (COD) was established (R² > 0.99). Further, combining this with numerical simulation in GMS revealed that the response pattern of the coefficient of dispersion to key parameters of freshwater lens development is as follows: freshwater appearance time > steady-state freshwater body thickness > steady-state freshwater reserve > lens stabilization time. These results clarify the development mechanism and formation process behind freshwater lenses on island reefs, from the micro to the macro scale, and provide a scientific basis for optimizing the protection of freshwater resources in coral islands and guiding the construction of artificial islands. Full article

To describe the fouling characteristics of compressor blades, fouling is categorized into dense and loose layers to characterize thickness and rough structures. An uncertainty model for dense fouling layer thickness distribution is constructed using the numerical integration and the Karhunen–Loève (KL) expansion method, while the Fouling Longuet-Higgins (FLH) model is proposed to address the uncertainty of loose fouling layer roughness. The FLH model effectively simulates the morphology characteristics of actual blade fouling and elucidates how parameters influence fouling roughness, morphology, and randomness. Based on the uncertainty modeling method, models for dense fouling layer thickness and loose fouling layer morphology are constructed, followed by numerical calculations and aerodynamic performance uncertainty quantification. Results indicate a 75.8% probability of aerodynamic performance degradation due to a dense fouling layer and a 97.2% probability related to the morphology uncertainty of a loose fouling layer when the roughness is 50 μm. This underscores that a mere focus on roughness is inadequate for characterizing blade fouling, and a comprehensive evaluation must also incorporate the implications of rough structures on aerodynamic performance. Full article

This study systematically analyzes the composition and microstructure of Neolithic pottery unearthed from the Dazhuzhuang, Likou, and Biting Sites in the Yongcheng District using techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), infrared spectroscopy (IR), and scanning electron microscopy with energy-dispersive spectroscopy (SEM-EDS). The results show that although the raw materials for pottery at the three sites were likely sourced from nearby ancient soil layers, significant differences in chemical composition and manufacturing techniques are evident. Pottery from the Dazhuzhuang Site is mainly composed of argillaceous gray pottery, with relatively loose raw material selection and a wide fluctuation in SiO₂ content (64.98–71.07%), reflecting diversity in raw material sources. At the Likou Site, argillaceous black pottery predominates, characterized by higher Al₂O₃ content (17.78%) and significant fluctuations in CaO content (1.46–2.22%), suggesting the addition of calcareous fluxes and the adoption of standardized manufacturing techniques. Pottery from the Biting Site mainly consists of argillaceous gray pottery, showing higher Al₂O₃ content (17.36%), stable SiO₂ content (65.19–69.01%), and the lowest CaO content (0.84–1.81%). The microstructural analysis further reveals that the black pottery (from the Likou Site) displays dense vitrified regions and localized iron enrichment. In contrast, the gray pottery (from the Dazhuzhuang and Biting Sites) shows clay platelet structures and vessel-type-specific differences in porosity. This research provides important scientific evidence for understanding raw material selection, manufacturing techniques, and regional cultural interactions in the Yongcheng area during the Longshan Culture period. Full article

Microalgae interact with mineral particles in an aqueous environment, yet how clay minerals affect physiological processes in algal cells remains unexplored. In this study, we compared the effects of palygorskite (Pal) and montmorillonite (Mt), which respectively represent fibrous and layered clay minerals, on the physiological processes of Chlamydomonas reinhardtii. It was observed that C. reinhardtii responded differently to the treatments of Pal and Mt. The Pal particles bound tightly to and even inserted themselves into cells, resulting in a significant decrease in cell numbers from 27.35 to 21.02 × 10⁷ mL⁻¹. However, Mt was only loosely attached to the cell surface. The photosynthesis in the algal cells was greatly inhibited by Pal, with the rETR_max significantly reduced from 103.80 to 56.67 μmol electrons m⁻²s⁻¹ and the downregulation of IF2CP, psbH and OHP1, which are key genes involved in photosynthesis. In addition, Pal reduced the quantities of proteins and polysaccharides in extracellular polymeric substances (EPSs) and the P uptake by C. reinhardtii when the P level in the culture was 3.15 mg/L. However, no significant changes were found regarding the above EPS components or the amount of P in algal cells upon the addition of Mt. Together, the impacts of fibrous Pal on C. reinhardtii was more profound than those of layered Mt. Full article

Background: The extracellular matrix (ECM), primarily composed of collagen and elastin synthesized by dermal fibroblasts, is critical for mesenchymal tissue integrity. Fibroblast phenotypes vary significantly with the anatomical location and developmental stage. Fetal skin, particularly prior to 14 weeks of gestation, exhibits a simplified structure compared to adult skin, characterized by a thin, loose dermal matrix and a single-layered epithelium. Objectives: This study aimed to characterize and functionally compare homogenous progenitor fetal fibroblast (PFF) populations derived from 14-week-old fetal skin with fibroblasts isolated from adult burn patients. Methods: We evaluated the proliferative capacity, collagen synthesis, and differentiation potential (adipogenesis and osteogenesis) of PFF and adult burn patient fibroblasts. Furthermore, we assessed their ability to support skin regeneration using a de-epidermized dermis (DED) model seeded with both PFF and patient-derived keratinocytes. The stability of PFF characteristics was monitored across multiple passages (P5–P12). Results: PFF demonstrated a 2–4-fold increase in proliferation rate and a 30–50% enhancement in collagen production in vitro compared to adult fibroblasts. Notably, PFF exhibited a consistent lack of adipogenic and osteogenic differentiation, an attribute distinct from adult fibroblasts. In the DED model, PFF, even at a low fibroblast-to-keratinocyte ratio (1:5), effectively facilitated the formation of well-organized skin structures, including rete ridges, surpassing the performance of adult fibroblasts and adipose-derived cells. These properties remained stable over multiple passages. Conclusions: The unique attributes of PFF, likely attributable to the simplified microenvironment (i.e., collagen organization) of developing fetal tissue, positions them as a promising source for cell-based therapies. Their inherent high collagen synthesis capacity is particularly advantageous for wound healing applications. Consequently, PFF represent a consistent and readily available resource for developing “off-the-freezer” cutaneous cell therapies, potentially enabling accelerated and improved treatment of severe burn injuries. Full article

This study assesses the risks of glacial lake outburst floods (GLOFs) from moraine sediment dams around Gurudongmar Lake in the Northern Sikkim Himalayas at an elevation of 17,800 feet. It focuses on three moraine sediment dams, analysing the implications of slope failure on the upstream side and the downstream stability under steady seepage conditions, as well as the risks posed by permafrost thawing. Using a comprehensive methodology that includes geotechnical evaluations, remote sensing, and digital elevation models (DEMs), the research employs finite element analysis via PLAXIS2D for the stability assessment. The main findings indicate a stratification of sediment types: the upper layers are loose silty sand, while the lower layers are dense silty sand, with significant variations in shear strength, permeability, and other geotechnical properties. Observations of solifluctions suggest that current permafrost conditions enhance the dams’ stability and reduce seepage. However, temperature trends show a warming climate, with the average days below 0 °C decreasing from 314 (2004–2013) to 305 (2014–2023), indicating potential permafrost thawing. This thawing could increase seepage and destabilise the dams, raising the risk of GLOFs. Numerical simulations reveal that scenarios involving water level rises of 5 and 10 m could lead to significant deformation and reduced safety factors on both the upstream lateral dams and downstream front dams. The study emphasises the urgent need for ongoing monitoring and risk assessment to address the potential hazards associated with GLOFs. Full article

Precise separation and antifouling capabilities are critical for the application of membrane separation technology. In this work, we developed a multiplayer layer-by-layer assembly strategy to sequentially deposit tannic acid (TA) and lysozyme (Lys) onto polyethersulfone/iron (PES/Fe) ultrafiltration membrane substrates, enabling the simple and efficient fabrication of a biofouling-resistant loose nanofiltration (LNF) membrane with superior dye/salt separation performance. This approach fully leverages the multifunctionality of TA by exploiting its coordination with Fe³⁺ and non-covalent interactions with Lys. The obtained PES/Fe-TA-Lys LNF membrane exhibits a pure water flux of 57.5 L·m⁻²·h⁻¹, along with exceptional dye rejection rates (98.3% for Congo Red (CR), 99.2% for Methyl Blue (MB), 98.4% for Eriochrome Black T (EBT), and 67.6% for Acid Orange 74 (AO74)) while maintaining minimal salt retention (8.2% for Na₂SO₄, 4.3% for MgSO₄, 3.5% for NaCl, and 2.4% for MgCl₂). The PES/Fe-TA-Lys LNF membrane also displays outstanding antifouling performance against bovine serum albumin (BSA), humic acid (HA), and CR, along with strong biofouling resistance against Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) via synergistic anti-adhesion and biofilm inhibiting effects. This work presents a novel and scalable approach to fabricating biofouling-resistant LNF membranes, offering great potential for dye/salt separation in textile wastewater treatment. Full article

Coccidian parasites possess complex life cycles involving asexual proliferation followed by sexual development, producing oocysts that are transmitted from host to host through feces, guaranteeing disease transmission. Eimeria necatrix is a highly pathogenic coccidian causing high mortality in birds. This study examined ultrastructural changes occurring during the third merogony, microgametogenesis, and macrogametogenesis of E. necatrix. The third-generation meront contained eight merozoites, each with coccidian-specific features like conoid, rhoptries, micronemes, and dense granules. Microgametes had a nucleus, mitochondrion, two flagella, and a basal apparatus. Macrogametes surrounded by two membranes (M1 and M2), contained organelles like WFB1, WFB2, endoplasmic reticulum, mitochondria, and tubular structures. Oocyst wall formation began with M2 separating from M1 and forming a loose veil around the organism. The WFB1 fused together to form the outer layer of the oocyst wall between M1 and M2, while M4 formed beneath M1. The WFB2 fused with the M4 to discharge its contents external to M4, which fused together to form the inner layer of the oocyst wall. Immunogold electron microscopy co-localization result showed that EnGAM22 localized to WFB1 and the outer wall, while EnGAM59 localized to WFB2 and the inner wall, suggesting they are key structural components of the oocyst wall. Full article

Tension-free hernioplasty has effectively reduced postoperative recurrence and mitigated complications by employing polymer patches. However, clinically used polymer patches often fall short in terms of the anti-deformation, anti-adhesion, and tissue integration functions, which can result in visceral adhesions and foreign body reactions after implantation. In this study, a Janus three-layer composite patch was developed for abdominal wall defect repair using a combination of 3D printing, electrospraying, and electrospinning technologies. On the visceral side, a dense electrospun polyvinyl alcohol/sodium hyaluronate (PVA/HA) scaffold was fabricated to inhibit cell adhesion. The middle layer, composed of polycaprolactone (PCL), provided mechanical support. On the muscle-facing side, a loose and porous electrospun nanofiber scaffold was created through electrospraying and electrospinning, promoting cell adhesion and migration to facilitate tissue regeneration. Mechanical testing demonstrated that the composite patch possessed excellent tensile strength (23.58 N/cm), surpassing the clinical standard (16 N/cm). Both in vitro and in vivo evaluations confirmed the patch’s outstanding biocompatibility. Compared with the control PCL patch, the Janus composite patch significantly reduced the visceral adhesion and enhanced the tissue repair in animal models. Collectively, this Janus composite patch integrated anti-deformation, anti-adhesion, and tissue-regenerative properties, providing a promising solution for effective abdominal wall defect repair. Full article

This study posits that soil particles in the filter layer are ellipsoidal. The effective pore radius of the filter material was calculated for various particle-shape parameters and distributions. The relationship between the porosity of the filter material and the ratio of the long axis to the short axis of ellipsoidal particles in a loose arrangement was also examined. The results indicate that the porosity of the filter material initially decreases and subsequently increases with increases in the ratio of the long axis to the short axis; however, the rate of increase progressively slows. A method for transforming irregularly shaped particles into ellipsoidal forms is proposed. The particle-shape parameter, S, is introduced to characterize the shape of irregular particles. The relationship between particle-shape parameters and the ratio of the long axis to the short axis was investigated specifically for ellipsoidal particles. It was found that the particle-shape parameters exhibit an approximately linear relationship with the ratio of the long axis to the short axis within a specific range. The discrete element method was employed to investigate the impact of particle shape on the filtration characteristics of the filter layer and was complemented by comparative experimental analysis. By analyzing the pore structures of spherical and ellipsoidal particles, this study predicts the relationship between pore structure and particle-shape parameters for any irregularly shaped natural-particle filter layer. Full article

Homogenized micro-crack crushing is an optimal rehabilitation technology for concrete pavement; however, when there are weak road base issues, some measures need to be taken to treat the diseases. Grouting is a common technique for addressing weak road base issues. This study developed a new visual indoor grouting test system to analyze the diffusion and consolidation of slag-based geopolymer slurry. The reactants of the geopolymer and the consolidation state of the slurry and aggregate were observed. Moreover, the reinforcement effect of the slurry on a weak road base was studied through the on-site grouting and excavation of the test pit. The results show that, during indoor grouting tests, as the size of the aggregate decreases, the slurry diffusion depth gradually decreases: only 9.5–4.75 mm aggregate formed a complete cylindrical specimen. In the tests of unformed cylindrical specimens, the 9.5–4.75 mm aggregate will develop 20–50 mm splitting surfaces, while the 4.75–2.36 mm aggregate will develop slurry bulbs and veins of different sizes, but the development is not obvious in the 2.36–1.18 mm aggregate. Fine aggregate grouting will exhibit the pressure filtration effect—especially for the 2.36–1.18 mm aggregate, the pressure filtration effect is the most obvious. An SEM microstructural analysis demonstrated that the geopolymer with a water–slag ratio of 0.4 has a faster hydration and dissolution, which results in a decrease in the density of local reactants. However, the polymerization of geopolymers is more complete. The pores of the coarse aggregate are larger and the slurry filling is denser, while the pores of the fine aggregate are smaller and the consolidation is loose locally. The consolidation of aggregates has cracks at local locations, but the width of the cracks is relatively small. On-site grouting applications revealed that the geopolymer slurry filled the bottom voids of pavement slabs and deep gaps in the road base layers, and the average deflection of the driveway decreased from 104.8 (0.001 mm) to 48 (0.001 mm) after grouting. Weak road base conditions were successfully treated, leading to a significant improvement in bearing capacity. Full article