Search Results (46)

Background: Piper is one of the largest genera in the family Piperaceae, with approximately 2100 species. Most Piper species are used as spices or as medicinal plants. Piper methysticum G. Forst., popularly known as kava-kava (or kava), is widely used to treat anxiety disorders. Due to similar morphological features, P. auritum Kunth (known as “false kava”) is sometimes mistakenly or intentionally used as an alternative botanical source for “kava” extracts. The false kava extracts do not contain active kavalactones but contain safrole, which is hepatotoxic. It is important to verify the component botanical materials in order to evaluate the quality and safety attributes of a potential botanical drug. Some studies have evaluated genetic variation in Piper sp. using the chloroplast regions matK, rbcL, rpoC1 and trnH-psbA and the nuclear ITS2 markers. However, none has focused on the identification of P. methysticum using DNA barcodes. In the present investigation, the ITS2 DNA barcode region from the nuclear genome was tested to confirm the identification and authentication of kava-kava samples. Methods: Seven P. methysticum samples were collected from three different geographic lo-cations and two P. auritum samples were collected and the ITS2 region from the nuclear genome, was amplified, sequenced and aligned to determine their genetic distances. Results: The ITS2 locus showed high amplification and sequence output with a discriminating barcode gap. A distance-based phylogenetic tree and BLAST confirmation (using blastn) revealed the ITS2 locus as a diagnostic DNA barcode for the accurate identification of kava-kava species. Discussion: In conclusion, the ITS2 region proves to be an effective and reliable DNA barcode for distinguishing P. methysticum from closely related species such as P. auritum. Its application can significantly improve the safety, quality, and traceability of kava-containing products, addressing a critical need in the standardization of botanical drugs. Full article

Piper methysticum G. Forst. (kava) is an important horticultural shrub of the Pacific, used as an ingredient of the intoxicating kava beverage, dietary supplement, and medicine around the globe. This study presents the first systematic evaluation of the phytochemical composition and nutrition values of roots and rhizomes of kava cultivated on three key kava-growing areas of Fiji (Rotuma, Kadavu, and Vanua Levu) by quantifying their kavalactone and flavokavain content, as well as measuring their calorific value, protein concentration and ash contents. Dried roots and rhizomes of the studied cultivars exhibited relatively high kavalactone concentrations (8.9–13.8 and 3.9–8.9 wt.%, respectively); favorable lactone profiles, with kavain as the major lactone component (2.1–4.6 and 1.1–2.6 wt.%, respectively); and low flavokavain contents (below 0.25 wt.%). The protein and ash contents of roots were measured to be 2.7–5.0 wt.% and 3.2–6.2 wt.%, respectively, and calorific values of roots were measured as 17.1–19.4 MJ·kg⁻¹—values that are systematically higher than those found for rhizomes (1.6–3.2 wt.%, 2.3–4.6 wt.%, and 16.5–17.7 MJ·kg⁻¹, respectively). A positive relationship between the calorific value and total kavalactone content was observed. A novel, unclassified kava cultivar (named Matanitabua) was discovered in Vanua Levu and identified as a noble kava cultivar. Full article

Industry 4.0 produces large volumes of sensor and machine data, offering new possibilities for manufacturing analytics but also creating challenges in combining domain knowledge with visual analysis. We present a systematic review of 13 peer-reviewed knowledge-assisted visual analytics (KAVA) systems published between 2014 and 2024, following PRISMA guidelines for the identification, screening, and inclusion processes. The survey is organized around six predefined building blocks, namely, user group, industrial domain, visualization, knowledge, data and machine learning, with a specific emphasis on the integration of knowledge and visualization in the reviewed studies. We find that ontologies, taxonomies, rule sets, and knowledge graphs provide explicit representations of expert understanding, sometimes enriched with annotations and threshold specifications. These structures are stored in RDF or graph databases, relational tables, or flat files, though interoperability is limited, and post-design contributions are not always persisted. Explicit knowledge is visualized through standard and specialized techniques, including thresholds in time-series plots, annotated dashboards, node–link diagrams, customized machine views from ontologies, and 3D digital twins with expert-defined rules. Line graphs, bar charts, and scatterplots are the most frequently used chart types, often augmented with thresholds and annotations derived from explicit knowledge. Recurring challenges include fragmented storage, heterogeneous data and knowledge types, limited automation, inconsistent validation of user input, and scarce long-term evaluations. Addressing these gaps will be essential for developing adaptable, reusable KAVA systems for industrial analytics. Full article

Various whitening cosmetics are available in the market, usually containing active whitening ingredients. However, most of the reported active ingredients have low dermal penetration due to their lipophilic structure. Therefore, it is necessary to develop effective whitening agents and novel formulations to address this. In previous studies, natural compounds such as chalcones have shown inhibitory effects on tyrosinase. However, most chalcone compounds have the disadvantage of poor water solubility, which restricts their dermal absorption. Flavokawain C (FKC) is a natural chalcone obtained from the root of the kava tree (Piper methysticum) and can also be obtained through organic synthesis. Since FKC is a chalcone, it is also water-insoluble, showing poor dermal absorption. In this study, electrospinning technology was used to develop FKC nanofibers (FKCNFs) to improve FKC’s physicochemical properties. The results showed that FKCNFs significantly improved water solubility and percutaneous absorption. Based on the results of in vitro experiments with B16F10 melanoma cells, 10 µM FKCNFs repressed the expressions of melanogenesis-related proteins MITF and TRP2. Furthermore, cosmetic safety assessment revealed that FKCNFs displayed a good margin of safety. This study suggests that FKCNFs have great potential as an effective active ingredient for whitening cosmetics. Full article

The kava beverage of the Pacific is known for its intoxicating, soothing and relaxing effects, attributed to a group of compounds (called kavalactones) produced by the kava plant. An unfavorable kavalactone profile, however, contributes to side effects, including hepatotoxicity. Therefore, the chemical quality of kava is of key importance for consumer safety. This work investigated the chemical quality of the kava cultivated on the Taveuni, Qamea, and Rabi Islands by quantifying the main kavalactones in the roots and rhizomes of eighty-eight kava plants using the HPLC method. It was concluded that the farmers in these regions almost exclusively grow classified noble kava cultivars with a favorable kavalactone profile; the total kavalactone concentration varies between 7.2–14.1 wt.% and 4.7–8.2 wt.% in the dried roots and rhizomes, respectively. Kavain is the major kavalactone component (27–34 wt.% of the total amount of kavalactones), followed by methysticin, yangonin, or dihydrokavain in the roots, and dihydrokavain or methysticin in the rhizomes, respectively. One unclassified non-noble cultivar, with elevated levels of non-desirable dihydrokavain, dihydromethysticin, and flavokavains, is also identified and characterized. Full article

Flavokavain B (FKB), a hepatotoxic chalcone from Piper methysticum (kava), has raised safety concerns due to its role in disrupting redox homeostasis and inducing apoptosis in hepatocytes. Conventional chromatographic methods for FKB detection, while sensitive, are costly and impractical for field applications. In this work, DNA aptamers were selected using the library-immobilized method and high-throughput sequencing. Three families of aptamers were obtained, and the best one named FKB-S showed a dissociation constant (K_D) of 280 nM using microscale thermophoresis. To demonstrate its practical utility, a rapid and label-free colorimetric aptasensor was developed based on aptamer-induced gold nanoparticle aggregation. This assay achieved a detection limit of 150 nM (43.46 ng/mL) and provided results within 10 min. Compared to traditional chromatographic methods, the aptasensor offers a simple, cost-effective, and equipment-free approach for on-site FKB detection, making it a promising tool for the quality control and safety monitoring of kava-based products in diverse environments. Full article

Background: Medication review is a multifaceted service aimed at optimizing the use of medicines and enhancing the health outcomes of patients. Due to its complexity, it is crucial to clearly describe the service, its variants, and its components to avoid confusion and ensure a better understanding of medication review among healthcare providers. Aim: This study aims to bring clarity to the origins, definitions, abbreviations, and types of medication reviews, together with the primary criteria that delineate key features of this service. Method: A narrative review approach was employed to clarify the diverse terminology associated with “medication review” services. Relevant references were initially identified through searches on PubMed and Google Scholar, complementing the existing literature known to the authors. Results: The study uncovers a complicated and sometimes convoluted history of “medication review” in different regions around the world. The initial optimization of medicine use had an economic purpose before evolving subsequently into a more patient-oriented approach. A selection of abbreviations, definitions, and types were outlined to enhance the understanding of the service. Conclusions: The study underscores the urgent need for comprehensive information and standardization regarding the content and quality of the services, collectively referred to as “medication review”. Full article

The fungal order Entomophthorales in the Zoopagomycota includes many fungal pathogens of arthropods. This review explores six genera in the subfamily Erynioideae within the family Entomophthoraceae, namely, Erynia, Furia, Orthomyces, Pandora, Strongwellsea, and Zoophthora. This is the largest subfamily in the Entomophthorales, including 126 described species. The species diversity, global distribution, and host range of this subfamily are summarized. Relatively few taxa are geographically widespread, and few have broad host ranges, which contrasts with many species with single reports from one location and one host species. The insect orders infected by the greatest numbers of species are the Diptera and Hemiptera. Across the subfamily, relatively few species have been cultivated in vitro, and those that have require more specialized media than many other fungi. Given their potential to attack arthropods and their position in the fungal evolutionary tree, we discuss which species might be adopted for biological control purposes or biotechnological innovations. Current challenges in the implementation of these species in biotechnology include the limited ability or difficulty in culturing many in vitro, a correlated paucity of genomic resources, and considerations regarding the host ranges of different species. Full article

Kava is the traditional intoxicating beverage of the Pacific with mild sedative and muscle relaxant effects, which are attributed to a group of compounds known as kavalactones. This paper aims to evaluate the quality of kava sold in the local markets of Fiji through the quantification of the six major kavalactones in kava root bundles and powdered kava packages using ethanolic extracts and HPLC. It was found in this work that kava root bundles contain mainly noble kava roots with a total kavalactone content of 8–13%; kavain had the highest concentration among kavalactones and kavain, methysticin, and yangonin together represented 69–71% of the total kavalactone content. Adulteration via mixing noble kava roots with those of non-noble kava with a relatively high dihydrokavain and dihydromethysticin content has also been observed. Powdered kava products were found to contain lower amounts of kavalactones (3–5%) with a less favorable kavalactone profile than those of root bundles, possibly due to mixing roots, rhizomes, and/or basal stems. The findings of this work, namely the variation in kavalactone content and profile in marketed products, indicate the need for rigorous quality control and quality indicators on kava commodities. Suggestions to include quantitative measures in the previously proposed chemical standardization code are also presented. Full article

Generalised Anxiety Disorder (GAD) is a prevalent, chronic mental health disorder. The measurement of regional brain gamma-aminobutyric acid (GABA) offers insight into its role in anxiety and is a potential biomarker for treatment response. Research literature suggests Piper methysticum (Kava) is efficacious as an anxiety treatment, but no study has assessed its effects on central GABA levels. This study investigated dorsal anterior cingulate (dACC) GABA levels in 37 adult participants with GAD. GABA was measured using proton magnetic resonance spectroscopy (¹H-MRS) at baseline and following an eight-week administration of Kava (standardised to 120 mg kavalactones twice daily) (n = 20) or placebo (n = 17). This study was part of the Kava for the Treatment of GAD (KGAD; ClinicalTrials.gov: NCT02219880), a 16-week intervention study. Compared with the placebo group, the Kava group had a significant reduction in dACC GABA (p = 0.049) at eight weeks. Baseline anxiety scores on the HAM-A were positively correlated with GABA levels but were not significantly related to treatment. Central GABA reductions following Kava treatment may signal an inhibitory effect, which, if considered efficacious, suggests that GABA levels are modulated by Kava, independent of reported anxiety symptoms. dACC GABA patterns suggest a functional role of higher levels in clinical anxiety but warrants further research for symptom benefit. Findings suggest that dACC GABA levels previously un-examined in GAD could serve as a biomarker for diagnosis and treatment response. Full article

In the United States (US), tianeptine is sold as a dietary supplement under the trade name Tianaa™. Tianeptine is a synthetic drug prescribed by physicians as an antidepressant in parts of Europe, Asia and South America. The drug is not permitted for use by physicians in the US, because it is a μ-opioid receptor agonist with a propensity for severe addiction. As the incidence of Tianaa™-related opioid addiction across the southern US escalates, the current study aimed to quantify tianeptine in over-the-counter Tianaa™ White, Red, and Green products. The results of this investigation measured tianeptine levels between 3.1 and 10.9 mg per 531 mg capsules. Tianaa™ White capsules consistently contained the least tianeptine, while Green had the most. The close inspection of Tianaa™ products showed that capsule mass varied by as much as 16% from label claim, and the amount of tianeptine per capsule varied by as much as 35% from the average measured amount for each product. Tianaa™ Red contains kava leaf extract, which led to the identification of four kavapyrone components by mass spectrometry. The data presented provide insight into tianeptine quantity and capsule mass variation for Tianaa™ supplements sold to customers naive to the risk of addiction. Full article

Lung cancer is the leading cause of cancer-related deaths due to its high incidence, late diagnosis, and limited success in clinical treatment. Prevention therefore is critical to help improve lung cancer management. Although tobacco control and tobacco cessation are effective strategies for lung cancer prevention, the numbers of current and former smokers in the USA and globally are not expected to decrease significantly in the near future. Chemoprevention and interception are needed to help high-risk individuals reduce their lung cancer risk or delay lung cancer development. This article will review the epidemiological data, pre-clinical animal data, and limited clinical data that support the potential of kava in reducing human lung cancer risk via its holistic polypharmacological effects. To facilitate its future clinical translation, advanced knowledge is needed with respect to its mechanisms of action and the development of mechanism-based non-invasive biomarkers in addition to safety and efficacy in more clinically relevant animal models. Full article

In children, diets high in sodium and low in potassium lead to increased blood pressure and risk of cardiovascular disease later in life. This study aimed to describe the major packaged food sources of sodium for New Zealand children aged 5 to 14 years and explored changes in the sodium content of these foods. Descriptive secondary analysis of 24 h dietary recall data from the 2002 National Children Nutrition Survey was undertaken to identify the major sources of sodium for children, and linear regression using Nutritrack data was used to assess changes in the sodium content of these foods from 2013 to 2019. For all NZ children, the major packaged food sources of sodium were white bread (contributing 23.1% to sodium intake),sausages (4.2%), ham (4.0%), canned spaghetti (3.5%) and wholemeal bread (3.2%). The results were comparable when analysed by gender, although boys attained more sodium from wholemeal bread and canned baked beans than girls (3.8% vs. 2.3% and 3.9% vs 1.8%, respectively). By age, white bread was the top contributor to sodium intake (25.6% for children aged 5–6 years and 22.6% for children aged 7–10 years and 11–14 years). Differences by ethnicity show that white bread made sodium contributions of 27.2% for Māori, 32.5% for Pacific and 20.6% for New Zealand European and others (NZEO). Sodium obtained from noodles was also higher among Māori and Pacific children (3.3% and 4.7%, respectively) than NZEO children (2.3%) as was sodium attained from extruded snacks and other crisps (Māori 2.0%, 2.5% Pacific and 0.9% NZEO). From 2013 to 2019, a significant decrease of 0.2 mg/100 g (95% CI: −70.2, −13.5) was found in the sodium content of white bread and whole hams and similar products (−0.4 mg/100 g, 95% CI: −636.2, −151.0) but an increase in the sodium content of noodles (0.2 mg/100 g, CI: 197.7, 703.2) over the same period of time. Understanding the major contributing packaged food sources of sodium and potassium for diverse New Zealand children is essential in protecting against future risk of cardiovascular disease and impetus for future public health initiatives aimed at reducing sodium intake. Full article

Epidemiological evidence suggests that kava (Piper methysticum Forst) drinks may reduce the risk of cancer in South Pacific Island smokers. However, little is known about the anti-carcinogenic effects of kava on tobacco smoking-related bladder cancer and its underlying mechanisms. Here we show that dietary feeding of kawain (a major active component in kava root extracts) to mice either before or after hydroxy butyl(butyl) nitrosamine (OH-BBN) carcinogen exposure slows down urinary bladder carcinogenesis and prolongs the survival of the OH-BBN-exposed mice. OH-BBN-induced bladder tumors exhibit significantly increased expression of lysine-specific demethylase 1 (LSD1), accompanied by decreased levels of H3K4 mono-methylation compared to normal bladder epithelium, whereas dietary kawain reverses the effects of OH-BBN on H3K4 mono-methylation. Human bladder cancer tumor tissues at different pathological grades also show significantly increased expression of LSD1 and decreased levels of H3K4 mono-methylation compared to normal urothelium. In addition, kava root extracts and the kavalactones kawain and methysticin all increase the levels of H3K4 mono- and di-methylation, leading to inhibitory effects on cell migration. Taken together, our results suggest that modification of histone lysine methylation may represent a new approach to bladder cancer prevention and treatment and that kavalactones may be promising agents for bladder cancer interception in both current and former smokers. Full article

Recent VLBI monitoring has found transverse motions of the M87 jet. However, due to the limited cadence of previous observations, details of the transverse motion have not been fully revealed yet. We have regularly monitored the M87 jet at KVN and VERA Array (KaVA) 22 GHz from December 2013 to June 2016. The average time interval of the observation is ∼0.1 year, which is suitable for tracking short-term structural changes. From these observations, the M87 jet is well represented by double ridge lines in the region 2–12 mas from the core. We found that the ridge lines exhibit transverse oscillations in all observed regions with an average period of $0.94\pm 0.12$ years. When the sinusoidal fit is performed, we found that the amplitude of this oscillation is an order of ∼0.1 mas, and the oscillations in the northern and southern limbs are almost in phase. Considering the amplitude, it does not originate from Earth’s parallax. We propose possible scenarios of the transverse oscillation, such as the propagation of jet instabilities or magneto-hydrodynamic (MHD) waves or perturbed mass injection around magnetically dominated accretion flows. Full article