Search Results (286)

Soil nutrient loss and infertility in rocky desertification areas severely constrain ecological restoration. Exploring the impacts of external field remediation technologies on soil quality in these regions may offer novel strategies for soil enhancement and ecosystem recovery. This study conducted a three-month experiment to investigate the impact of continuous electric (ET, 20 V) and magnetic (MT, 200 mT) field treatments on soil nutrients, enzyme activities, and bacterial communities in simulated moderate and severe rocky desertification soils. Results showed that although an overall declining trends in total contents of key soil nutrients (Total nitrogen, total phosphorus, and total potassium), both electric and magnetic field treatments effectively mitigated the decreases of total nitrogen and potassium content (with the exception of total phosphorus) in rocky desertification soils, while improving their available contents compared to the control (CK). Electric field application significantly reduced the pH of moderate and severe rocky desertification soils through electrolysis, shifting the soil from alkaline (pH 7.69 and 7.73, respectively) to slightly acidic (pH 6.71 and 6.37, respectively); Both electric and magnetic field treatments enhanced urease and sucrase activities in moderately and severely rocky desertified soils. Compared to the CK, the increases were 21.92%, 4.46%, 5.70%, and 66.43% in moderately rocky desertified soil, and 10.06%, 42.15%, 20.66%, and 0.93% in severely rocky desertified soil, respectively. Their effects on phosphatase and catalase activities varied with the degree of rocky desertification. However, in severely rocky desertified soil, both treatments significantly increased phosphatase and catalase activities by 19.55%, 24.63%, 61.07%, and 38.05% compared to the CK, respectively. Furthermore, both electric and magnetic treatments significantly reduced bacterial α-diversity (chao1, ACE, Shannon, Simpson, and Pielou J indices) but optimized community structure by enriching dominant phyla with specific ecological functions, such as Pseudomonadota (7.63–41.10%), Bacteroidota (13.52–69.29%), and Verrucomicrobiota (38.26–104.81%). Functional prediction revealed that the abundances of dominant pathways (such as chemoheterotrophy, aerobic chemoheterotrophy, and nitrogen fixation) was enhanced following both treatments. Mantel analysis further indicated strengthened correlations among soil nutrients, enzyme activities, and bacterial communities, particularly under magnetic field treatment. These findings demonstrate that electric and magnetic field applications effectively facilitate nutrient cycling, stimulate enzyme activities, and optimize microbial community structure, thereby improving soil ecological functions and overall quality in rocky desertification regions, highlighting their potential for ecological restoration in karst areas. Full article

Alternanthera philoxeroides is a highly invasive alien species in China that causes waterway blockages, agricultural yield loss, biodiversity decline, and ecosystem degradation. This study assessed the invasion risk and environmental drivers of A. philoxeroides in Guizhou Province, a karst mountainous region in Southwest China. Occurrence records were obtained from field surveys and the Chinese Virtual Herbarium. The genetic algorithm for rule-set production (GARP) model and the jackknife method were employed to identify 13 key environmental indicators for predicting invasion risk. The global invasion risk index (GIRI) was applied to quantify the overall invasion risk. Additionally, the Geodetector model was utilized to analyze the spatially differentiated effects of six environmental factors. The results showed that A. philoxeroides poses a high invasion risk in Guizhou Province, and the invasion risk in the Yangtze River Basin within Guizhou is higher than that in the Pearl River Basin. The environmental factors influencing invasion risk, in order of impact, were slope, elevation, land use, river density, rocky desertification, and soil pH. Moreover, interactions among these factors further amplify the invasion risk. These findings provide valuable insights for developing targeted management strategies for A. philoxeroides in karst mountainous regions and support biodiversity preservation and regional ecological sustainability. Full article

Although the coupling coordination relationship (CCR) between ecological environment and economic development has received extensive scholarly attention, investigations into the underlying mechanisms of this coupling coordination remain insufficient. Taking the Chishui River Basin (CRB) in Southwest China as the study area, this study integrates remote sensing data and county-level statistical datasets. Firstly, the quality of the ecological environment and economic development level of the CRB are systematically evaluated. Secondly, an improved coupling coordination degree model (ICCDM) is adopted to quantify the CCR between the ecological environment and economic development, as well as its spatiotemporal evolution characteristics. Finally, an obstacle degree model and panel Tobit model are employed to explore the influencing factors of the CCR from both intrinsic and extrinsic perspectives. The results show that during the study period, both the ecological environment index (EEI) and the economic development index (EDI) in the CRB exhibited upward trends, with pronounced inter-county disparities. The CCR between ecological environment and economic development was continuously optimized, and the coupling coordination degree (CCD) displayed a distinct spatial gradient pattern of downstream regions > midstream regions > upstream regions. Obstacle degree analysis identifies significant heterogeneity in the obstacle factors for CCR improvement across the basin: Renhuai and Zunyi are dominated by ecological environment constraints, while 11 counties including Chishui and Xishui are mainly restricted by economic development constraints. Industrial structure, ecological endowment, industrialization level and government capacity are vital positive driving factors for the CCR in the CRB, whereas Terrain conditions act as a key negative restraining factor. This study indicates that the overall coupling coordination level between ecological environment and economic development in the CRB is still relatively low and requires further enhancement. Therefore, region-specific differentiated regulation strategies are urgently needed to achieve high-level coordinated development between the ecological environment and economy in the CRB. Full article

The accumulation dynamics and regulatory mechanisms of the alkylamides, the key pungent compounds in the fruits of Sichuan peppers, remain poorly understood. Using fruits of the Zanthoxylum planispinum var. dintanensis (Dintan) harvested at five key developmental stages, we comprehensively mapped the accumulation of numbering compounds and their underlying molecular drivers by integrating HPLC-based metabolite profiling and de novo transcriptomics. Total alkylamide content increased during development, with hydroxyl-α-sanshool (HαSS) being predominant. The contributions of hydroxyl-β-sanshool (HβSS) and hydroxyl-ε-sanshool (HεSS) increased in later stages. Cluster and correlation analyses identified 51 candidate genes strongly correlated (|r| ≥ 0.6) with HαSS accumulation, predominantly enriched in fatty acid and branched-chain amino acid metabolism pathways. The expression patterns of five stearoyl-CoA desaturase (SCD) genes, one long-chain acyl-CoA synthetase (ACSL/fadD), and one S-(hydroxymethyl)glutathione dehydrogenase/alcohol dehydrogenase (frmA) gene closely mirrored HαSS accumulation. In contrast, 3-oxoacyl-[acyl-carrier-protein] synthase II (fabF) and one β-ketoacyl-CoA synthase (KCS) gene exhibited a negative correlation. Accordingly, a positive regulatory network was constructed for HαSS accumulation. These findings revealed key candidate targets for deciphering the molecular basis of its unique flavor and for breeding high-pungency cultivars. Full article

Karst rock desertification is an extreme form of land degradation that poses a serious threat to regional ecological security and sustainable development in Southwest China. Understanding the response patterns of plant communities and soil properties along desertification gradients is critical for developing effective ecological restoration strategies. This study focused on Qingzhen City, Guizhou Province, a representative karst desertification region. Using remote sensing to classify rock desertification intensity, together with systematic vegetation surveys and soil sampling, we investigated variation patterns in ecological parameters along the degradation gradient. The results revealed three key patterns. First, rock desertification was widespread across Qingzhen and exhibited pronounced spatial differentiation. Second, as desertification intensified, vegetation community structure became progressively simplified, transitioning from forests to shrublands. Biomass and vegetation cover declined from 77.25 kg/m² and 83% to 0.62 kg/m² and 15%, respectively. Notably, species diversity exhibited a bell-shaped relationship with desertification intensity, peaking at the potential desertification stage before declining under increasing environmental stress. Third, soil physicochemical properties showed complex nonlinear responses along the desertification gradient. Soil bulk density decreased, and pH increased with increasing desertification intensity, while volumetric water content fluctuated across stages. Soil total carbon and total nitrogen exhibited temporary enrichment during the light-to-moderate desertification stages, likely due to shifts in litter quality. Overall, these findings demonstrate that both plant communities and soil properties respond nonlinearly to rock desertification gradients. Together, the results enhance the understanding of the ecological processes underlying karst rock desertification and support the development of targeted regional restoration strategies. Full article

Geometric morphometrics is an important component of quantitative research on insect morphology, widely applied in taxonomy, intraspecific variation, and phylogenetic studies. However, systematic research in this field remains limited, with few comprehensive summaries of research trends, hotspots, and core theories. This study, based on scientometric methods, analyzed 1321 publications indexed in the Web of Science database up to 31 December 2025, and presents a meta-scientific review from a macro perspective, revealing the research trends, hotspots, and future directions in the field. The results show that: (1) annual publications exhibit overall growth, while research methods evolved from single landmark analysis to multimodal and interdisciplinary approaches; (2) scientists from Brazil, the USA, and France are major contributors, with studies spanning morphology, taxonomy, and ecology; (3) taxonomic studies centered on wing shape analysis constitutes a major research hotspot, closely related to phylogeny, allometry, and sexual dimorphism; (4) highly co-cited studies provide the main theoretical and methodological foundations for the field. Future research, building on existing hotspots, will further integrate geometric morphometrics with genomics, ecological functional data, three-dimensional geometric morphometrics, and artificial intelligence-assisted approaches to advance integrative taxonomy within interdisciplinary and data-driven frameworks. Full article

Zanthoxylum planispinum var. dingtanensis (hereafter Z. planispinum) is a pioneer plant for the ecological restoration of karst rocky desertification, offering both ecological rehabilitation and economic benefits. Due to the combined pressure from soil degradation and fruit harvesting, nutrient depletion and quality decline have intensified. Therefore, investigating the effects of fertilization on pericarp yield and quality provides the scientific basis for its precise fertilization. This study examined a Z. planispinum plantation subjected to five treatments in 2021: no fertilization (CK); organic fertilizer + chemical fertilizer + sprinkler irrigation (T1); chemical fertilizer + sprinkler irrigation (T2); chemical fertilizer alone (T3); and legume (soybean) intercropping + chemical fertilizer + sprinkler irrigation (T4). It explored the intrinsic relationships between fruit quality and soil physicochemical properties, identified key soil factors, and conducted a comprehensive quality evaluation, providing a scientific basis for precise fertilization in karst regions. The results indicated that (1) whole-fruit water content ranged from 61.30% to 64.37%, showing no significant differences, while variations were observed in the other phenotypic traits. T1 exhibited the highest values for Hydroxy-β-sanshool (2.42 mg·g⁻¹), Hydroxy-ε-sanshool (0.80 mg·g⁻¹), essential oil content (8.57%), and fresh weight per plant (9.9 kg). After long-term soybean intercropping, pericarp thickness reached its maximum (0.45 mm), but the content of aroma compounds decreased significantly. Compared to the other four treatments, the unfertilized control (CK) showed significantly higher values for the pericarp dry weight ratio, proportion of closed-eye peppercorns, and relative content of d-limonene, with increases of 5.5–13.94%, 130.91–568.42%, and 8.74–14.46%, respectively. (2) An inhibitory effect was observed between pericarp numbing compounds and soil calcium/phosphorus levels, while the synthesis of aroma compounds was constrained by the soil C/N ratio. Soil P/K ratio was identified as the dominant factor affecting overall quality. (3) The comprehensive fruit quality index ranked as follows: T1 (1.2933) > T3 (0.666) > T2 (0.5285) > CK (−1.1555) > T4 (−1.2098). Therefore, the T1 treatment is recommended for promotion as the fertilization management practice for Z. planispinum plantations in karst regions. Full article

Different forest ecosystems affect the acquisition and loss of SOC by changing the niche differentiation of above-ground and under-ground, resulting in changes in the utilization efficiency of water and nutrient elements. The impact of different types of forests on carbon storage in forest soils has received significant attention in recent decades, as these ecosystems are critical for mitigating the effects of global climate change. There are significant differences in environmental factors among different types of forests, such as carbon source type, topographic characteristics, soil texture, microbial community status, climate and hydrological conditions. At present, the research on the effects of environmental factors such as climate, hydrological conditions or soil quality on SOC has been well carried out. Nevertheless, the distribution pattern of microbial carbon and particulate organic carbon in subtropical forest ecosystems and their contribution to SOC still need much of scientific research. Forest types have a significant impact on the content and distribution characteristics of MNC and particulate organic carbon fractions, but there is heterogeneity in different forests. Importantly, the random forest analysis showed that MNC and MAOC were the main factors affecting SOC compared with other variables, which indicated MNC and MAOC have higher relative importance to SOC (p < 0.05). Specifically, our research found that the total MNC and BNC content in natural forests and broad-leaved forests were significantly higher than that in coniferous forests (p < 0.05), while the FNC content and FNC/BNC in coniferous forests were significantly higher than that in the other two forests (p < 0.05). In addition, the MAOC content of natural forests was higher than others, which indicated the stability of natural forest is higher than other forests. However, CPOC, FPOC content, and POC/MAOC in coniferous forests were significantly higher than in broad-leaf forests and natural forests. Biotic and abiotic factors profoundly affect the dynamic changes in SOC accumulation and stability. Different environmental factors lead to more MNC and MAOC in forest types with faster decomposition rates. These findings have instructive implications for understanding the contributions of different forest types on SOC stability and accumulation mechanisms in forest soils. Full article

Background: The adaptability of leguminous plant–rhizobia symbionts enables enhanced plant stress tolerance in environmentally stressed areas. However, how rock desertification (RD) severity affects the endophytic and nitrogen-fixing bacterial communities in Pisum sativum root nodules remains unclear. Methods: We systematically surveyed the microbial communities of P. sativum nodules across a gradient of four RD areas. We sequenced 16S rRNA and nifH amplicons, determined soil physicochemical properties, and performed bioinformatic analyses to relate nodule microbiome diversity to soil variables. Results: The dominant endophytic genera across all sites were Allorhizobium–Neorhizobium–Pararhizobium–Rhizobium and Pseudomonas, with Rhizobium identified as the primary nitrogen-fixing taxon. Soil pH and total phosphorus (TP) showed significant correlations with the overall endophytic bacterial community, whereas total nitrogen (TN), TP, and soil water content (SWC) were associated with nitrogen-fixing taxa. Notably, P. sativum nodules from areas of slight rocky desertification (SRD) harbored higher endophytic bacterial diversity and enhanced carbohydrate metabolism compared to those from moderately rocky desertified (MRD) sites. Conclusions: This study sheds light on how bacterial communities within legume root nodules respond to RD stress, deepening our understanding of plant–microbe co-adaptation and informing microbial-assisted restoration strategies in karst desertification areas. Full article

Karst desertification (KD) severely constrains regional ecological security and sustainable development. As an important ecological restoration measure, soil and water conservation agroforestry (SWCAF) systems have unclear mechanisms for soil microbial responses. This study investigated the effects of potential–light (PL), light–moderate (LM), and moderate–high (MH) KD on soil physicochemical properties and microbial communities in Karst SWCAF (KSWCAF) systems. It explored the drivers of microbial community changes. The results showed that (1) Soil physicochemical properties exhibited nonlinear changes along the KD gradient. Key soil-fertility indicators including silt, clay, total porosity (TP), total phosphorus (Total_P), total nitrogen (Total_N), soil organic carbon (SOC), and carbon nitrogen ratio (C_N) showed significant unimodal patterns, peaking at the LM stage with optimal overall soil quality; (2) The dominant bacterial phyla were Pseudomonadota, Acidobacteriota, Actinomycetota, and Planctomycetota, while the dominant fungal phyla were Ascomycota, Basidiomycota, and Mortierellomycota. The overall abundance of these dominant phyla increased with intensifying KD, except that the relative abundance of Pseudomonadota was lowest in the QZ study area, while Acidobacteriota was highest in the QZ area. The dominant fungal phylum Ascomycota increased with KD intensification; (3) KD significantly influenced microbial community structure and beta diversity. Fungi showed stronger responses to the KD gradient than bacteria. Bacterial alpha diversity was significantly higher in the LM stage compared to the PL and MH stages (p < 0.05), while fungal alpha diversity was significantly lowest in the MH stage (p < 0.05); (4) Bacterial networks exhibited highest complexity but reduced stability at the LM stage, whereas fungal networks enhanced stability at the MH stage by increasing modularization and positive correlation proportions; (5) RDA revealed that soil physicochemical factors explained 66.89% and 98.82% of bacterial and fungal community variation, respectively, with pH, moisture, and C_N as key drivers. Overall, KD regulates microbial community structure and functional allocation by reshaping the soil environmental gradient, with the LM stage potentially representing a “transitional optimization window” for KSWCAF ecosystem structure and function. This study provides a theoretical basis for microbial regulation strategies in KD control and soil and water conservation (SWC) processes. Full article

The fragile Karst landscapes of southwest China face persistent challenges of soil degradation and rocky desertification. While sustainable land use such as mulberry plantation can support ecological restoration, the dynamics of soil organic carbon (SOC) and its driving mechanisms across contrasting soil types remain poorly understood, limiting the development of targeted pedogenically aware carbon management strategies. A comparative field study was conducted in central Guizhou, China, over an eight-month mulberry growing season (April to November). We monitored SOC, physicochemical properties, GRSP, and enzyme activities in plantations established on two contrasting limestone-derived soils (Calcisols and Chromic Luvisols). This study aimed to clarify the relationships between SOC and key soil parameters within each soil type and to identify their dominant driving factors. Soil type significantly influenced SOC concentration, dynamics, and its regulatory mechanisms. SOC was significantly higher and exhibited greater seasonal variability in Calcisols (31.51–39.71 g·kg⁻¹) than in Chromic Luvisols (22.50–28.51 g·kg⁻¹), with Calcisols maintaining 1.28–1.57 times the SOC concentration of Chromic Luvisols. Regression analysis revealed that SOC was significantly positively correlated with TN, AN, AK, and GRSP, but significantly negatively correlated with AP. Random forest modeling further identified distinct key correlated factors in each soil type as follows: TN, T-GRSP, and Urease were primary in Calcisols, whereas TN, T-GRSP, and pH dominated in Chromic Luvisols. Partial least squares path modeling confirmed that soil type does not directly associate with SOC but exerts an indirect effect by modulating core biochemical mediators specifically (Alkaline protease, T-GRSP, and TN); The model also indicated that pH and TN exert direct positive effects on SOC accumulation. In Karst mulberry systems, pedogenically distinct soils (Calcisols vs. Chromic Luvisols) shape SOC storage, stability, and regulatory mechanisms through divergent biogeochemical pathways. SOC management should therefore be soil-type-specific, prioritizing nitrogen synergy in Calcisols and pH-mediated stabilization in Chromic Luvisols, rather than applying uniform strategies. This study thereby establishes a mechanistic framework for understanding and managing SOC in heterogeneous Karst landscapes, providing a critical foundation for developing targeted, soil-specific carbon sequestration practices in ecologically vulnerable regions. Full article

China boasts abundant cultivated resources of pitahaya, with Guizhou Province being one of its core producing areas. Quality differences in red-fleshed pitahaya among local producing areas have not been fully clarified, and a standardized quantitative evaluation system for these differences remains lacking. This study seeks to identify the key factors influencing regional variations in quality and establish a comprehensive evaluation standard. In this study, 15 samples of red-fleshed pitahaya were collected from four major producing areas in Guizhou and used as research materials. Based on 15 quality characteristic indicators of the fruits, an analysis of quality differences and establishment of an evaluation system were carried out using multivariate statistical analysis. The results showed that 14 of the 15 quality indicators exhibited significant differences among pitahaya samples from different producing areas (p < 0.05), with the a* value being the sole exception. Cluster analysis classified the 15 samples into four groups. Principal component analysis (PCA) extracted four principal components, with a cumulative variance contribution rate of 81.07%, which clearly identified betacyanin, betaxanthin, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging rate, vitamin C, fruit shape index, and transverse diameter as the core evaluation indicators. This study systematically clarifies the differences in quality characteristics and the internal correlations among quality indicators of red-fleshed pitahaya from different major producing areas in Guizhou. It further provides an important scientific basis for pitahaya variety breeding, cultivation regulation, and market positioning in this region and fills the research gap existing in the field of comprehensive quality evaluation of pitahaya. This is of significant practical importance for promoting the standardized upgrading of local specialty fruit industries, enhancing the market competitiveness of products, and facilitating the high-quality development of the agricultural economy. Full article

In southwest China, vegetation restoration is widely used in karst rocky desertification control projects. However, mechanistic evidence explaining how plant community composition and species diversity regulate cadmium (Cd) bioavailability remains limited. Here, the plant community’s species diversity, soil properties, Cd, and available Cd contents were evaluated. Four plant community types, NR (natural recovery), PMC (Pistacia weinmannifolia + Medicago sativa + Chrysopogon zizanioides), and PME (Pistacia weinmannifolia + Medicago sativa + Eragrostis curvula), were selected as the research objects. The species composition was recorded, and dominant plant species and soil samples were collected to analyze Cd accumulation characteristics. Relative to NR, composite restorations increased plant diversity and soil nutrient availability and reduced soil compaction, with PMC showing the strongest remediation, decreasing total Cd by 49.4% and available Cd by 59.5%. Model-averaged regression and hierarchical partitioning analyses further identified nitrogen availability and community structure as the dominant drivers. Specifically, available nitrogen (AN), vegetation coverage, Margalef species richness (DMG), ammonium nitrogen (NH₄⁺–N), and total N (TN) were the main factors of soil total Cd, and BD, TN, nitrate nitrogen (NO₃⁻–N), mean crown diameter (MCD), and Shannon–Wiener index (H′) were the main factors of soil available Cd. The results indicate that PMC provides a plant community structure configuration decisions of a scalable, site-adaptable strategy for durable Cd stabilization and soil conservation in thin, carbonate-rich karst soils. Full article

The Rocky Desertification area has high sensitivity and poor anti-interference ability in the ecosystem. It is challenging to achieve sustainable development in a rocky desertification area. Given this issue, the System Dynamics model, the Future Land Use Simulation (FlUS) model, the Integrated Valuation and Trade-offs of ESs (InVEST) model, and the Structural Equation Model (SEM) were integrated in this study to analyze future ecosystem service change in Wenshan Prefecture under SSP1-1.9, SSP2-4.5, and SSP5-8.5 scenarios. The following results are obtained. (1) The area of cultivated land, construction land, forest land, and grassland increased in SSP1-1.9; the area of forest land and grassland decreased in SSP2-4.5 scenario and SSP5-8.5 scenario. (2) The water supply (WS), carbon sequestration (CS), and soil conservation power (SDR) under the three different scenarios were improved compared with 2020. Among them, habitat quality (HQ) demonstrated a slight increase trend under the SSP1-1.9 scenario but decreased under the other two scenarios. (3) WS, CS, and HQ exhibited a tradeoff relationship in the three scenarios compared with 2020. (4) In the SSP1-1.9 and SSP2-4.5 scenarios, the synergistic relationships among CS, HQ, SDR, and WS were particularly detected in the northern, southern, and central parts of the study area. Additionally, climate change and vegetation-dominated ecological environment are the main driving mechanisms affecting ES changes. This paper summarizes the spatial differences in the change trend and synergistic tradeoff and lays a crucial scientific foundation for the ecological protection of karst landform areas. Full article

Guizhou Province is located in the karst area of southern China, where rocky desertification is severe, and habitat fragmentation is prominent. Leafhoppers are widely distributed, with over 2000 recognized species found in China. However, the impact of habitat fragmentation in karst areas on the biodiversity of leafhoppers has not been comprehensively explored. Leafhopper specimens were collected in Bijie City, Guizhou Province (a light to moderate karst rocky desertification area) from 2019 to 2022. Using methods such as the generalized linear model (GLM) and redundancy analysis (RDA), this study explored the impacts of habitat fragmentation and environmental factors (plants, soil, climate) on the genus-level and genetic diversity of leafhoppers. When the degree of habitat fragmentation decreased, the genus diversity of leafhoppers increased significantly. The GLM showed that among the characteristics of fragmented habitats, patch area had the greatest impact on the generic richness of leafhoppers. RDA indicated that gene exchange among leafhoppers was more frequent between patches with irregular boundaries, and leafhoppers in patches with small areas and complex boundaries had greater genetic diversity. As phytophagous insects, leafhoppers were mainly affected by host plants and less affected by soil properties. Mitigating habitat fragmentation had a positive impact on the biodiversity of leafhoppers, which emphasizes the necessity of protecting biodiversity by reducing habitat fragmentation in the future. Full article