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15 pages, 2881 KiB  
Article
Monitoring Rose Black Spot Disease Using Electrical Impedance Spectroscopy
by Tianyi Ma, Dongyu Tan, Rui Wang, Tianyi Li, Yiying Wang, Guilin Shan, Ji Qian and Bao Di
Agronomy 2025, 15(8), 1800; https://doi.org/10.3390/agronomy15081800 - 25 Jul 2025
Viewed by 178
Abstract
Rosa hybrida is a globally important ornamental species, but its economic and aesthetic value is often compromised by rose black spot disease (Diplocarpon rosae). Effective monitoring and early detection are essential for disease management. This study investigated physiological and biophysical responses [...] Read more.
Rosa hybrida is a globally important ornamental species, but its economic and aesthetic value is often compromised by rose black spot disease (Diplocarpon rosae). Effective monitoring and early detection are essential for disease management. This study investigated physiological and biophysical responses to infection in a resistant cultivar (‘Carefree Wonder’) and a susceptible cultivar (‘Red Cap’) using electrical impedance spectroscopy (EIS), biochemical assays, and ultrastructural analysis. Key EIS parameters (ri, re, τ), reducing sugar and free proline content, chitinase and β-1,3-glucanase activities, and chloroplast ultrastructure were monitored. The results showed that ‘Carefree Wonder’ had a higher initial EIS arc magnitude and osmolyte levels than ‘Red Cap’. Following infection, ‘Red Cap’ displayed earlier and more pronounced increases in EIS arc magnitude, while ‘Carefree Wonder’ responded more gradually. Reducing sugar and proline levels increased in both cultivars, with earlier accumulation in the resistant cultivar. Notably, extracellular resistivity (re) exhibited strong positive correlations with reducing sugar (R2 = 0.479), free proline (R2 = 0.399), chitinase (R2 = 0.399), and β-1,3-glucanase activities (R2 = 0.401). These findings highlight re as the most reliable EIS-derived indicator for early, non-destructive detection of rose black spot resistance. This study supports the potential of EIS for rapid disease diagnostics in rose breeding and cultivation. Full article
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20 pages, 7197 KiB  
Article
Simulation of Water–Energy–Food–Carbon Nexus in the Agricultural Production Process in Liaocheng Based on the System Dynamics (SD)
by Wenshuang Yuan, Hao Wang, Yuyu Liu, Song Han, Xin Cong and Zhenghe Xu
Sustainability 2025, 17(14), 6607; https://doi.org/10.3390/su17146607 - 19 Jul 2025
Viewed by 384
Abstract
To achieve regional sustainable development, the low-carbon transformation of agriculture is essential, as it serves both as a significant carbon source and as a potential carbon sink. This study calculated the agricultural carbon emissions in Liaocheng from 2010 to 2022 by analyzing processes [...] Read more.
To achieve regional sustainable development, the low-carbon transformation of agriculture is essential, as it serves both as a significant carbon source and as a potential carbon sink. This study calculated the agricultural carbon emissions in Liaocheng from 2010 to 2022 by analyzing processes including crop cultivation, animal husbandry, and agricultural input. Additionally, a simulation model of the water–energy–food–carbon nexus (WEFC-Nexus) for Liaocheng’s agricultural production process was developed. Using Vensim PLE 10.0.0 software, this study constructed a WEFC-Nexus model encompassing four major subsystems: economic development, agricultural production, agricultural inputs, and water use. The model explored four policy scenarios: business-as-usual scenario (S1), ideal agricultural development (S2), strengthening agricultural investment (S3), and reducing agricultural input costs (S4). It also forecast the trends in carbon emissions and primary sector GDP under these different scenarios from 2023 to 2030. The conclusions were as follows: (1) Total agricultural carbon emissions exhibited a three-phase trajectory, namely, “rapid growth (2010–2014)–sharp decline (2015–2020)–gradual rebound (2021–2022)”, with sectoral contributions ranked as livestock farming (50%) > agricultural inputs (27%) > crop cultivation (23%). (2) The carbon emissions per unit of primary sector GDP (CEAG) for S2, S3, and S4 decreased by 8.86%, 5.79%, and 7.72%, respectively, compared to S1. The relationship between the carbon emissions under the four scenarios is S3 > S1 > S2 > S4. The relationship between the four scenarios in the primary sector GDP is S3 > S2 > S4 > S1. S2 can both control carbon emissions and achieve growth in primary industry output. Policy recommendations emphasize reducing chemical fertilizer use, optimizing livestock management, enhancing agricultural technology efficiency, and adjusting agricultural structures to balance economic development with environmental sustainability. Full article
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22 pages, 3439 KiB  
Article
Metabolomics Analysis Reveals the Influence Mechanism of Different Growth Years on the Growth, Metabolism and Accumulation of Medicinal Components of Bupleurum scorzonerifolium Willd. (Apiaceae)
by Jialin Sun, Jianhao Wu, Weinan Li, Xiubo Liu and Wei Ma
Biology 2025, 14(7), 864; https://doi.org/10.3390/biology14070864 - 16 Jul 2025
Viewed by 230
Abstract
Bupleurum scorzonerifolium Willd. is a perennial herbaceous plant of the genus Bupleurum in the Apiaceae family. Also known as red Bupleurum, it is mainly distributed in Northeast China, North China and other regions and is a commonly used medicinal plant. It is [...] Read more.
Bupleurum scorzonerifolium Willd. is a perennial herbaceous plant of the genus Bupleurum in the Apiaceae family. Also known as red Bupleurum, it is mainly distributed in Northeast China, North China and other regions and is a commonly used medicinal plant. It is difficult for the wild plant resources of Bupleurum scorzonerifolium Willd. to meet the market demand. In artificial cultivation, there are problems such as a low yield per plant, low quality, weakened stress resistance and variety degradation. The contents of bioactive components and metabolites in traditional Chinese medicinal materials vary significantly across different growth years. The growth duration directly impacts their quality and clinical efficacy. Therefore, determining the optimal growth period is one of the crucial factors in ensuring the quality of traditional Chinese medicinal materials. In this study, Gas Chromatography–Mass Spectrometry (GC-MS) and High-performance liquid chromatography (HPLC) were comprehensively applied to analyze the metabolically differential substances in different parts of Bupleurum scorzonerifolium Willd. By comparing the compositions and content differences of chemical components in different growth years and different parts, the chemical components with significant differences were accurately screened out. In order to further explore the dynamic change characteristics and internal laws of metabolites, a metabolic network was constructed for a visual analysis and, finally, to see the optimal growth years of Bupleurum scorzonerifolium Willd. This result showed that with the accumulation of the growth cycle, the height, root width, fresh mass and saikosaponins content of Bupleurum scorzonerifolium Willd. increased year by year. Except for sodium and calcium elements in the main shoot, the other elements were significantly reduced. In addition, 59 primary metabolites were identified by GC-MS, with the accumulation of the growth cycle, the contents of organic acids, sugars, alcohols and amino acids gradually decreased, while the contents of alkyl, glycosides and other substances gradually increased. There were 53 positive correlations and 18 negative correlations in the triennial Bupleurum scorzonerifolium Willd. grid, all of which were positively correlated with saikosaponins. Therefore, the triennial Bupleurum scorzonerifolium Willd. was considered to be the suitable growth year. It not only provided a new idea and method for the quality evaluation of Bupleurum scorzonerifolium Willd., but also provided a scientific basis for the quality control of Chinese herbs. Full article
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43 pages, 532 KiB  
Article
The Study of Practice in the Vehicles of Men and Gods and the Bodhisattva Vehicle: Perspectives from Master Taixu to Master Hsing Yun
by Chienhuang Chen
Religions 2025, 16(7), 910; https://doi.org/10.3390/rel16070910 - 15 Jul 2025
Viewed by 357
Abstract
This paper explores the perspectives of Master Taixu (太虛, 1890–1947) and Master Hsing Yun (星雲, 1927–2023) on the practice of the Buddhist Five Vehicles (五乘), particularly focusing on the relationship between the Vehicles of Men and Gods (人天乘) and the Bodhisattva Vehicle (菩薩乘). [...] Read more.
This paper explores the perspectives of Master Taixu (太虛, 1890–1947) and Master Hsing Yun (星雲, 1927–2023) on the practice of the Buddhist Five Vehicles (五乘), particularly focusing on the relationship between the Vehicles of Men and Gods (人天乘) and the Bodhisattva Vehicle (菩薩乘). Master Taixu advocated “Buddhism for Human Life” (人生佛教), emphasizing the improvement of real life as the foundation for Buddhist practice in daily life. He proposed the principle of “following the practice and fruition of the Human Vehicle to cultivate the Mahāyāna path” (依人乘行果,趣修大乘行), suggesting a gradual progression from the human vehicle to the Mahāyāna Bodhisattva path. He introduced the concept of “The perfection of human character leads to Buddhahood” (人圓佛即成) and “Present Bodhisattva Practice” (今菩薩行), highlighting the importance of adapting Buddhist practice to contemporary society and actively engaging in social service. Master Hsing Yun’s advocacy of “Humanistic Buddhism” (人間佛教) emphasizes the diversity of practice, asserting that any action aligned with Buddhist teachings, beneficial to human well-being, and conducive to the welfare of all beings constitutes a form of practice, thereby expanding its definition. He elaborated on the Five Vehicles, viewing the Vehicle of Men and Gods as a preliminary stage, advocating a gradual progression towards the Bodhisattva Vehicle, ultimately culminating in Buddhahood. He proposed the principle of “undertaking worldly affairs with an otherworldly mindset” (以出世的思想,做入世的事業), highlighting the importance of practicing the Bodhisattva path in everyday life. With “Buddha Light Pure Land” (佛光淨土) as the ultimate goal, he sought to integrate the essence of various Pure Lands traditions to achieve earthly peace and purification. Both masters highlight the importance of practicing Humanistic Buddhism, advocating for the application of the Bodhisattva practice in daily life, guiding all beings with compassion and wisdom, and actively engaging in society to address real-world issues, ultimately realizing a Humanistic Pure Land. However, their perspectives also differ: Master Taixu focused more on the theoretical construction and an exposition on the order of practice, whereas Master Hsing Yun placed greater emphasis on the diversity and flexibility of practice and the integration of Buddhist teachings into modern life. Full article
8 pages, 192 KiB  
Perspective
Diversification of Rice-Based Cropping Systems with Vegetables and Legumes in Asia and Africa
by Ramasamy Srinivasan
Crops 2025, 5(4), 43; https://doi.org/10.3390/crops5040043 - 14 Jul 2025
Viewed by 768
Abstract
Rice is one of the most important staple foods worldwide. Asia, particularly South and Southeast Asia, is a major rice producer, and rice production is also gradually increasing in Africa. However, rice cultivation poses economic and environmental challenges, which are exacerbated by climate [...] Read more.
Rice is one of the most important staple foods worldwide. Asia, particularly South and Southeast Asia, is a major rice producer, and rice production is also gradually increasing in Africa. However, rice cultivation poses economic and environmental challenges, which are exacerbated by climate change. Hence, diversification of rice-based production systems is highly imperative to improve soil health and thus sustain productivity while also enhancing income opportunities. Vegetables and pulses are crucial components for diversifying rice-based production systems as they have the potential to increase income and improve soil health. The World Vegetable Center has introduced mungbeans and vegetable soybeans to diversify the cereal-based production systems in Asia. About 27–93% of the mungbean area in India, Pakistan, Bangladesh, and Myanmar is planted with varieties containing improved germplasm developed by WorldVeg in collaboration with national agricultural research systems. Additionally, the introduction of vegetables and legumes is highly remunerative and improves dietary diversity, leading to better nutrition. For instance, the productivity of vegetable crops increased by 200–350% when they were combined with improved production practices. Such diversification also holds great promise for improving income and nutrition in Africa. It also enhances the resilience of farming systems, particularly in a changing climate. Hence, governments should prioritize system diversification to enhance the income and livelihood opportunities for smallholders in Asia and Africa. Full article
21 pages, 10356 KiB  
Article
Autonomous Greenhouse Cultivation of Dwarf Tomato: Performance Evaluation of Intelligent Algorithms for Multiple-Sensor Feedback
by Stef C. Maree, Pinglin Zhang, Bart M. van Marrewijk, Feije de Zwart, Monique Bijlaard and Silke Hemming
Sensors 2025, 25(14), 4321; https://doi.org/10.3390/s25144321 - 10 Jul 2025
Viewed by 431
Abstract
Greenhouse horticulture plays an important role globally by producing nutritious fruits and vegetables with high resource use efficiency. Modern greenhouses are large-scale high-tech production factories that are increasingly data-driven, and where climate and irrigation control are gradually becoming more autonomous. This is enabled [...] Read more.
Greenhouse horticulture plays an important role globally by producing nutritious fruits and vegetables with high resource use efficiency. Modern greenhouses are large-scale high-tech production factories that are increasingly data-driven, and where climate and irrigation control are gradually becoming more autonomous. This is enabled by technological developments and driven by shortages in skilled labor and the demand for improved resource use efficiency. In the Autonomous Greenhouse Challenge, it has been shown that controlling greenhouse cultivation can be done efficiently with intelligent algorithms. For an optimal strategy, however, it is essential that control algorithms properly account for crop responses, which requires appropriate sensors, reliable data, and accurate models. This paper presents the results of the 4th Autonomous Greenhouse Challenge, in which international teams developed six intelligent algorithms that fully controlled a dwarf tomato cultivation, a crop that is well-suited for robotic harvesting, but for which little prior cultivation data exists. Nevertheless, the analysis of the experiment showed that all teams managed to obtain a profitable strategy, and the best algorithm resulted a production equivalent to 45 kg/m2/year, higher than in the commercial practice of high-wire cherry tomato growing. The predominant factor was found to be the much higher plant density that can be achieved in the applied growing system. More difficult challenges were found to be related to measuring crop status to determine the harvest moment. Finally, this experiment shows the potential for novel greenhouse cultivation systems that are inherently well-suited for autonomous control, and results in a unique and rich dataset to support future research. Full article
(This article belongs to the Special Issue AI, IoT and Smart Sensors for Precision Agriculture: 2nd Edition)
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18 pages, 10178 KiB  
Article
Effects of Legume–Grass Mixture Combinations and Planting Ratios on Forage Productivity and Nutritional Quality in Typical Sand-Fixing Vegetation Areas of the Mu Us Sandy Land
by Yuqing Mi, Hongbin Xu, Lei Zhang, Ruihua Pan, Shengnan Zhang, Haiyan Gao, Haibing Wang and Chunying Wang
Agriculture 2025, 15(14), 1474; https://doi.org/10.3390/agriculture15141474 - 9 Jul 2025
Viewed by 376
Abstract
Monoculture and legume–grass mixed cropping are the two most common planting methods, with mixed cropping generally demonstrating higher hay yield and superior nutritional quality compared to monoculture. However, research on legume–grass mixed cropping for establishing cultivated pastures in typical sand-fixing vegetation areas of [...] Read more.
Monoculture and legume–grass mixed cropping are the two most common planting methods, with mixed cropping generally demonstrating higher hay yield and superior nutritional quality compared to monoculture. However, research on legume–grass mixed cropping for establishing cultivated pastures in typical sand-fixing vegetation areas of the Mu Us Sandy Land remains scarce. These knowledge gaps have hindered the synergistic integration of forage production and ecological restoration in the region. This study conducted mixed cropping trials in the sand-fixing vegetation zone of the Mu Us Sandy Land using Dahurian wildrye (Elymus dahuricus), Mongolian wheatgrass (Agropyron mongolicum), and Standing milkvetch (Astragalus adsurgens) to investigate the effects of species combinations and planting ratios on forage productivity and nutritional quality, aiming to determine the optimal planting strategy. Results showed that in the first establishment year, the yield of all mixed cropping systems significantly exceeded that of monocultured Dahurian wildrye and Mongolian wheatgrass. All mixed cropping combinations exhibited land equivalent ratios (LER) and relative yield totals (RYT) below 1, indicating varying degrees of interspecific competition during the first year, with grass species generally demonstrating stronger competitive abilities than legumes. Mixed-cropped forages showed higher crude protein, crude fat, and crude ash content compared to monocultures, alongside lower neutral detergent fiber (NDF) and acid detergent fiber (ADF) levels, suggesting improved relative feed value (RFV). Among the combinations, E5A5 and E6A4 (5:5 and 6:4 ratio of Dahurian wildrye to Standing milkvetch) achieved higher RFV, with RFV gradually declining as the legume proportion decreased. In conclusion, both monoculture and legume–grass mixed cropping are viable in the Mu Us Sandy Land’s sand-fixing vegetation areas and the E5A5 combination (5:5 ratio of Dahurian wildrye to Standing milkvetch) as having the highest overall score, demonstrating that this mixed cropping ratio optimally balances yield and nutritional quality, making it the recommended planting protocol for the region. This mixed cropping system offers a theoretical foundation for efficiently establishing artificial pastures in the Mu Us Sandy Land, supporting regional pastoral industry development and desertification mitigation. Full article
(This article belongs to the Section Crop Production)
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29 pages, 22994 KiB  
Article
Simulating Land Use and Evaluating Spatial Patterns in Wuhan Under Multiple Climate Scenarios: An Integrated SD-PLUS-FD Modeling Approach
by Hao Yuan, Xinyu Li, Meichen Ding, Guoqiang Shen and Mengyuan Xu
Land 2025, 14(7), 1412; https://doi.org/10.3390/land14071412 - 4 Jul 2025
Viewed by 434
Abstract
Amid intensifying global climate anomalies and accelerating urban expansion, land use systems have become increasingly dynamic, complex, and uncertain. Accurately predicting and scientifically evaluating the evolution of land use patterns is essential to advancing territorial spatial governance and achieving ecological security goals. However, [...] Read more.
Amid intensifying global climate anomalies and accelerating urban expansion, land use systems have become increasingly dynamic, complex, and uncertain. Accurately predicting and scientifically evaluating the evolution of land use patterns is essential to advancing territorial spatial governance and achieving ecological security goals. However, most existing land use models emphasize quantity forecasting and spatial allocation, while overlooking the third critical dimension—structural complexity, which is essential for understanding the nonlinear, fragmented evolution of urban systems, thus limiting their ability to fully capture the evolutionary characteristics of urban land systems. To address this gap, this study proposes an integrated SD-PLUS-FD model, which combines System Dynamics, Patch-based Land Use Simulation, and Fractal Dimension analysis to construct a comprehensive three-dimensional framework for simulating and evaluating land use patterns in terms of quantity, spatial distribution, and structural complexity. Wuhan is selected as the case study area, with simulations conducted under three IPCC-aligned climate scenarios—SSP1-2.6, SSP2-4.5, and SSP5-8.5—to project land use changes by 2030. The SD model demonstrates robust predictive performance, with an overall error of less than ±5%, while the PLUS model achieves high spatial accuracy (average Kappa >0.7996; average overall accuracy >0.8856). Fractal dimension analysis further reveals that since 2000, the spatial boundary complexity of all land use types—except forest land—has generally shown an upward trend across multiple scenarios, highlighting the increasingly nonlinear and fragmented nature of urban expansion. The FD values for construction land and cultivated land declined to their historical low in 2005, then gradually increased, reaching their peak under the SSP1-2.6 scenario. Notably, the increase in FD for construction land was significantly greater than that for cultivated land, indicating a stronger dynamic response in spatial structural evolution. In contrast, forest land exhibited pronounced scenario-dependent variations in FD. Its structural complexity remained generally stable under all scenarios except SSP5-8.5, reflecting higher structural resilience and boundary adaptability under diverse socioclimatic conditions. The SD-PLUS-FD model effectively reveals how land systems respond to different socioclimatic drivers in both spatial and structural dimensions. This three-dimensional framework reveals how land systems respond to socioclimatic drivers across temporal, spatial, and structural scales, offering strategic insights for climate-resilient planning and optimized land resource management in rapidly urbanizing regions. Full article
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40 pages, 3175 KiB  
Review
The Causative Agent of Soft Rot in Plants, the Phytopathogenic Bacterium Pectobacterium carotovorum subsp. carotovorum: A Brief Description and an Overview of Methods to Control It
by Alla I. Perfileva, Elena I. Strekalovskaya, Nadezhda V. Klushina, Igor V. Gorbenko and Konstantin V. Krutovsky
Agronomy 2025, 15(7), 1578; https://doi.org/10.3390/agronomy15071578 - 28 Jun 2025
Viewed by 702
Abstract
This review presents information obtained over the past 10 years on the methods to control the widespread worldwide phytopathogen Pectobacterium carotovorum subsp. carotovorum (Pcc). This bacterium is among the ten most dangerous phytopathogens; it affects a wide range of cultivated plants: [...] Read more.
This review presents information obtained over the past 10 years on the methods to control the widespread worldwide phytopathogen Pectobacterium carotovorum subsp. carotovorum (Pcc). This bacterium is among the ten most dangerous phytopathogens; it affects a wide range of cultivated plants: vegetables, ornamental and medicinal crops, both during vegetation and during the storage of fruits. Symptoms of Pcc damage include the wilting of plants, blackening of vessels on leaves, stems and petioles. At the flowering stage, the stem core gradually wilts and, starting from the root, the stem breaks and the plant dies. Pcc is a rod-shaped, non-capsule and endospore-forming facultative anaerobic Gram-negative bacterium with peritrichous flagellation. Pcc synthesizes bacteriocins—carocins. The main virulence factors of Pcc are the synthesis of N-acyl-homoserine lactone (AHL) and plant cell wall-degrading enzymes (PCWDEs) (pectinases, polygalacturonases, cellulases, and proteases). Diagnostic methods for this phytopathogen include polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), multilocus genotyping of strain-specific genes and detection of unique volatile organic compounds (VOCs). The main methods to control this microorganism include the use of various chemicals (acids, phenols, esters, salts, gases), plant extracts (from grasses, shrubs, trees, and algae), antagonistic bacteria (Bacillus, Pseudomonas, Streptomyces, and lactic acid bacteria), viruses (including a mixture of bacteriophages), and nanomaterials based on metals and chitosan. Full article
(This article belongs to the Section Pest and Disease Management)
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24 pages, 2363 KiB  
Article
Influence of Environmental Conditions Associated with Low and High Altitudes on Economic and Quality Characteristics of Fruit Ripening of Camellia chekiangoleosa Hu
by Teng Wei, Shengyue Zhong, Bin Huang, Kang Zha, Jing Li and Qiang Wen
Foods 2025, 14(13), 2266; https://doi.org/10.3390/foods14132266 - 26 Jun 2025
Cited by 1 | Viewed by 337
Abstract
Camellia chekiangoleosa Hu. (C. chekiangoleosa) is a typical high-altitude oil-tea Camellia species. Due to altitude being an important factor affecting crop growth and quality, the influence of environmental conditions associated with low (60 m) and high (600 m) altitudes on the [...] Read more.
Camellia chekiangoleosa Hu. (C. chekiangoleosa) is a typical high-altitude oil-tea Camellia species. Due to altitude being an important factor affecting crop growth and quality, the influence of environmental conditions associated with low (60 m) and high (600 m) altitudes on the economic and quality characteristics of fruit ripening was assessed in this study. Our investigations showed that altitude has no influence on the growth pattern of C. chekiangoleosa fruit shells and seed oils, and the differences in samples between different altitudes gradually decreased with the ripening of C. chekiangoleosa. Nevertheless, mature C. chekiangoleosa fruit shells and seed oils from low and high altitudes showed some differences. Specifically, the fruit shells of C. chekiangoleosa cultivated in low-altitude areas contained more soluble sugar, protein, total polyphenols, total flavonoids, and tea saponin. Meanwhile, low-altitude cultivation elevated the abundance of α-tocopherol, β-sitosterol, β-amyrinol, flavonoids, and polyphenols in mature seed oils but decreased the oil yield. Moreover, few effects of altitude on fatty acid composition were observed in mature seed oils. Cluster and receiver operating characteristic (ROC) analysis indicated that the influence of altitude on the quality of mature seed oils was strongly associated with oil yield and α-tocopherol. Taken together, the present study suggests that when cultivating C. chekiangoleosa in low-altitude regions, more energy should be devoted to improving oil yield. The results of the fruiting process and quality trait variation in C. chekiangoleosa during the low-altitude introduction process can provide an important theoretical basis for the introduction and cultivation of this oil-tea species. Full article
(This article belongs to the Section Plant Foods)
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22 pages, 2762 KiB  
Article
Foliar Application of Melatonin Improves Photosynthesis and Secondary Metabolism in Chenopodium quinoa Willd. Seedlings Under High-Temperature Stress
by Meiqing Li, Jinyang Li, Deke Xing and Yanyou Wu
Agronomy 2025, 15(7), 1556; https://doi.org/10.3390/agronomy15071556 - 26 Jun 2025
Viewed by 268
Abstract
The suitable growth environment for quinoa is high-altitude areas. In recent years, quinoa is also gradually cultivated in other regions with high-temperature exposure. High-temperature stress poses a potential constraint on quinoa quality and yield by impacting pigments, photosynthesis, and metabolites. This study aimed [...] Read more.
The suitable growth environment for quinoa is high-altitude areas. In recent years, quinoa is also gradually cultivated in other regions with high-temperature exposure. High-temperature stress poses a potential constraint on quinoa quality and yield by impacting pigments, photosynthesis, and metabolites. This study aimed to investigate the effect of exogenous melatonin (MT) in alleviating heat stress on quinoa in controllable conditions. Day/night temperatures were maintained at 35/25 °C in a climate chamber, and foliar spraying was performed using melatonin (MT) concentrations of 0, 50, 100, and 200 μmol L−1. Day/night temperatures were maintained at 25/15 °C in another climate chamber as a comparative trial. Our results demonstrated that high temperature decreased the levels of photosynthetic pigments and the values of photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr). Additionally, it also influenced the accumulation of polyphenols and altered polyphenol oxidase (PPO) activity in the red quinoa (RQ) cultivar. Obvious reductions in gas exchange parameters and metabolites including flavonoid, anthocyanin, and PPO were observed both in the BQ cultivar and the WQ cultivar. However, the application of 100 μmol L−1 MT significantly increased the levels of photosynthetic pigments, the values of Pn, gs, and Tr, and the PPO activity, as well as the contents of flavonoid and anthocyanin in the RQ cultivar. The application of 50 μmol L−1 MT only led to an increase in the concentrations of Chl a, Chl (a + b), and flavonoids, as well as PPO activity, whereas 100 μmol L−1 MT significantly enhanced the values of Pn, gs, and Tr and the PPO activity. Additionally, 200 μmol L−1 MT contributed to the synthesis of anthocyanins and polyphenols, and enhanced PPO activity in the BQ cultivar. The application of 50 μmol L−1 MT limited the increase in the contents of total polyphenols, flavonoids, and anthocyanin, we all as PPO activity, in the WQ cultivar. The findings demonstrated that photosynthesis and metabolite synthesis in quinoa under high temperatures depends on an interactive response between cultivar and melatonin levels. The application of 100 μmol L−1 MT was found to be optimal for alleviating the adverse effects of high temperature on photosynthesis and metabolites in the RQ cultivar during actual production. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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17 pages, 2766 KiB  
Article
Dietary Astragalus Polysaccharides Can Improve the Immune Capacity and Reproductive Performance of the Lined Seahorse (Hippocampus erectus)
by Siping Li, Xin Liu, Tingting Lin, Yuanhao Ren, Dong Zhang and Keji Jiang
Biology 2025, 14(7), 767; https://doi.org/10.3390/biology14070767 - 25 Jun 2025
Viewed by 442
Abstract
Seahorse (Hippocampus spp.) is popular in the markets of traditional Chinese medicine, aquarium, and curio. In order to protect wild stocks and still meet the market demand, China attempted the large-scale cultivation of seahorses in the early 21st century and achieved it [...] Read more.
Seahorse (Hippocampus spp.) is popular in the markets of traditional Chinese medicine, aquarium, and curio. In order to protect wild stocks and still meet the market demand, China attempted the large-scale cultivation of seahorses in the early 21st century and achieved it in the 2010s. However, in recent years, two new issues have gradually emerged in Chinese seahorse cultivation. One is that the juveniles are prone to disease during diet conversion, and the other is that the reproductive performance of broodstocks is significantly reduced. With the aim to provide some measures that can alleviate these issues, in the present study, we used lined seahorse (Hippocampus erectus, a species widely cultured in China) as the experimental subject and Astragalus polysaccharides (APSs) as the immunostimulant to test whether APSs could improve the immune-health status and reproductive performance of seahorses. The measured indices for reproductive performance included ovarian lipid content, assessment time required before mating for paired male and female seahorses, mating success rate, brood size, and newborn body height. The results showed that for juveniles during diet conversion, their body weight, survival rate, plasma immunocytokine contents (interleukin-2, interferon-α, and immunoglobulin M), and alpha diversity indices (Simpson and Pielou’s-e) of the intestinal microbiota were significantly higher than those of the control group after dietary APSs. For broodstocks, compared with the control group, the expression of lipid substances in the ovary was significantly upregulated, the assessment time was significantly shortened, and the body height of their newborns was significantly increased in the APS group. These results demonstrate that APSs could indeed improve the immune-health status and reproductive performance of seahorses, providing guidance for addressing existing issues in seahorse cultivation. Full article
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20 pages, 4795 KiB  
Article
Assessment of Crop Water Resource Utilization in Arid and Semi-Arid Regions Based on the Water Footprint Theory
by Yuqian Tang, Nan Xia, Yuxuan Xiao, Zhanjiang Xu and Yonggang Ma
Agronomy 2025, 15(7), 1529; https://doi.org/10.3390/agronomy15071529 - 24 Jun 2025
Viewed by 245
Abstract
The arid and semi-arid regions of Northwest China, as major agricultural production zones, have long faced dual challenges: increasing water resource pressure and severe supply–demand imbalances caused by the expansion of cultivated land. The crop water footprint, an effective indicator for quantifying agricultural [...] Read more.
The arid and semi-arid regions of Northwest China, as major agricultural production zones, have long faced dual challenges: increasing water resource pressure and severe supply–demand imbalances caused by the expansion of cultivated land. The crop water footprint, an effective indicator for quantifying agricultural water use, plays a crucial role in supporting sustainable development in the region. This study adopted a multi-scale spatiotemporal analysis framework, combining the CROPWAT model with Geographic Information System (GIS) techniques to investigate the spatiotemporal evolution of crop water footprints in Northwest China from 2000 to 2020. The Logarithmic Mean Divisia Index (LMDI) model was used to analyze spatial variations in the driving forces. A multidimensional evaluation system—encompassing structural, economic, ecological, and sustainability dimensions—was established to comprehensively assess agricultural water resource utilization in the region. Results indicated that the crop water footprint in Northwest China followed a “decline-increase-decline” trend, it increased from 90.97 billion m3 in 2000 to a peak of 133.49 billion m3 in 2017, before declining to 129.30 billion m3 in 2020. The center of the crop water footprint gradually shifted northward—from northern Qinghai to southern Inner Mongolia—mainly due to rapid farmland expansion and increasing water consumption in northern areas. Policy and institutional effect, together with economic development effect, were identified as the primary drivers, contributing 49% in total. Although reliance on blue water has decreased, the region continues to experience moderate water pressure, with sustainable use achieved in only half of the study years. Water scarcity remains a pressing concern. This study offers a theoretical basis and policy recommendations to enhance water use efficiency, develop effective management strategies, and promote sustainable water resource utilization in Northwest China. Full article
(This article belongs to the Section Water Use and Irrigation)
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23 pages, 615 KiB  
Article
Can New Quality Productivity Drive the Low-Carbon Transformation of Carbon-Intensive Industries? Macro and Micro Evidence from China
by Hui Wang, Jie Zhou, Kuiying Gu and Feng Dong
Energies 2025, 18(13), 3278; https://doi.org/10.3390/en18133278 - 23 Jun 2025
Viewed by 366
Abstract
Reducing carbon dioxide emissions within carbon-intensive industries is a critical strategy to effectively combat global warming. The accelerated cultivation and enhancement of new quality productivity has created new momentum directed towards industrial low-carbon transformation. Using data from a sample of Chinese provinces and [...] Read more.
Reducing carbon dioxide emissions within carbon-intensive industries is a critical strategy to effectively combat global warming. The accelerated cultivation and enhancement of new quality productivity has created new momentum directed towards industrial low-carbon transformation. Using data from a sample of Chinese provinces and enterprises between 2011 and 2022, this study quantifies, evaluates, and explores the influence and mechanisms of new quality productivity on the low-carbon transformation of carbon-intensive industries. The research findings show that: (1) Fostering new quality productivity effectively promotes the low-carbon transformation of carbon-intensive industries and plays a positive, empowering role. Industrial innovation, digital stimulation, technological innovation, and green empowerment all support the low-carbon transformation of carbon-intensive industries, with their respective impacts gradually decreasing in turn. (2) Mechanism analysis confirms a chain transmission mechanism of “new quality productivity—environmental protection investment—green innovation—the transformation of carbon-intensive industries” at the macro-provincial level. In micro-level carbon-intensive enterprises, a positive U-shaped relationship between new quality productivity and low-carbon transformation of carbon-intensive industries is evident, and the main pathways include increasing low-carbon, energy-saving investment and improving the ESG performance of high-carbon emission enterprises. (3) Advancing transformation is more pronounced in central and western areas, high-carbon areas, non-carbon trading pilot areas, and non-energy-rich ecologically fragile areas. The government and enterprises should take advantage of the development opportunities of new quality productivity and adopt low-carbon behaviors to promote transformational development. Full article
(This article belongs to the Section C: Energy Economics and Policy)
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24 pages, 3837 KiB  
Article
The Alleviating Effect of Brassinosteroids on Cadmium Stress in Potato Plants: Insights from StDWF4 Gene Overexpression
by Xiangyan Zhou, Rong Miao, Jiaqi Luo, Wenhui Tang, Kexin Liu, Caijuan Li and Dan Zhang
Agronomy 2025, 15(7), 1503; https://doi.org/10.3390/agronomy15071503 - 20 Jun 2025
Viewed by 485
Abstract
The potato is the fourth largest cultivated crop worldwide. Soil cadmium (Cd) pollution poses a significant threat to crop growth. Brassinosteroids (BRs) play a significant part in enhancing plant resistance against abiotic stresses. The DWF4 (dwarf4) gene is one of the rate-limiting enzyme [...] Read more.
The potato is the fourth largest cultivated crop worldwide. Soil cadmium (Cd) pollution poses a significant threat to crop growth. Brassinosteroids (BRs) play a significant part in enhancing plant resistance against abiotic stresses. The DWF4 (dwarf4) gene is one of the rate-limiting enzyme genes involved in the synthesis of BRs. This study employed seedlings of transgenic potatoes overexpressing the StDWF4 gene (OE) and wild-type (WT) potatoes to clarify their alleviating effect on Cd stresses. The differences in phenotype, ultrastructure, physiological indicators, and plant hormone levels of Cd2+-treated potatoes were analyzed. The molecular mechanism of potatoes’ response to Cd2+ stress was revealed by transcriptomics. Results showed that the dry weight, fresh weight, plant height, root length, and stem diameter of OE potatoes under Cd stress were significantly higher than those of WT potatoes. Ultrastructural analysis revealed that the mitochondria, cell walls, and cell membranes of WT were more fragile than those of OE under Cd stress. The Cd2+ concentration in OE was always lower than that in WT, and both concentrations increased gradually as the duration of Cd2+ treatment was prolonged. The 24-epibrassionlide (EBL) content in OE was higher than that in WT. RNA-seq analysis manifested that the gene expression levels of OE and WT plants changed significantly under Cd2+ treatment. The differentially expressed genes (DEGs) were primarily connected to the moderation of the metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and plant hormone signal transduction. These findings indicated that overexpression of the StDWF4 gene in potatoes enhanced their alleviating effect on Cd stresses. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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