Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (1,369)

Search Parameters:
Keywords = germination time

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
15 pages, 1899 KiB  
Article
Heterologous Watermelon HSP17.4 Expression Confers Improved Heat Tolerance to Arabidopsis thaliana
by Yajie Hong, Yurui Li, Jing Chen, Nailin Xing, Wona Ding, Lili Chen, Yunping Huang, Qiuping Li and Kaixing Lu
Curr. Issues Mol. Biol. 2025, 47(8), 606; https://doi.org/10.3390/cimb47080606 (registering DOI) - 1 Aug 2025
Abstract
Members of the heat shock protein 20 (HSP20) family of proteins play an important role in responding to various forms of stress. Here, the expression of ClaHSP17.4 was induced by heat stress in watermelon. Then, a floral dipping approach was used to introduce [...] Read more.
Members of the heat shock protein 20 (HSP20) family of proteins play an important role in responding to various forms of stress. Here, the expression of ClaHSP17.4 was induced by heat stress in watermelon. Then, a floral dipping approach was used to introduce the pCAMBIA1391b-GFP overexpression vector encoding the heat tolerance-related gene ClaHSP17.4 from watermelon into Arabidopsis thaliana, and we obtained ClaHSP17.4-overexpressing Arabidopsis plants. Under normal conditions, the phenotypes of transgenic and wild-type (WT) Arabidopsis plants were largely similar. Following exposure to heat stress, however, the germination rates (96%) of transgenic Arabidopsis plants at the germination stages were significantly higher than those of wild-type idopsis (17%). Specifically, the malondialdehyde (MDA) content of transgenic Arabidopsis was half that of the control group, while the activities of peroxidase (POD) and superoxide dismutase (SOD) were 1.25 times those of the control group after exposure to high temperatures for 12 h at the seedling stages. The proline content in ClaHSP17.4-overexpressing transgenic Arabidopsis increased by 17% compared to WT plants (* p < 0.05), while the soluble sugar content rose by 37% (* p < 0.05). These results suggest that ClaHSP17.4 overexpression indirectly improves the antioxidant capacity and osmotic regulatory capacity of Arabidopsis seedlings, leading to improved survival and greater heat tolerance. Meanwhile, the results of this study provide a reference for further research on the function of the ClHSP17.4 gene and lay a foundation for breeding heat-tolerant watermelon varieties and advancing our understanding of plant adaptation to environmental stress. Full article
Show Figures

Figure 1

16 pages, 1659 KiB  
Article
Ricinus communis L. Leaf Extracts as a Sustainable Alternative for Weed Management
by Aline Mazoy Lopes, Lucas Kila Ribeiro, Maurício Ricardo de Melo Cogo, Lucas Mironuk Frescura, Marcelo Barcellos da Rosa, Alex Schulz, Flávio Dias Mayer, Ederson Rossi Abaide, Marcus Vinícius Tres and Giovani Leone Zabot
Sustainability 2025, 17(15), 6942; https://doi.org/10.3390/su17156942 (registering DOI) - 30 Jul 2025
Viewed by 128
Abstract
Weeds pose a significant challenge to agricultural productivity, requiring control strategies that are both effective and environmentally sustainable. Therefore, this study evaluated the inhibitory potential of aqueous extracts from Ricinus communis L. leaves to manage the weeds Oryza sativa L. (weedy rice) and [...] Read more.
Weeds pose a significant challenge to agricultural productivity, requiring control strategies that are both effective and environmentally sustainable. Therefore, this study evaluated the inhibitory potential of aqueous extracts from Ricinus communis L. leaves to manage the weeds Oryza sativa L. (weedy rice) and Cyperus ferax. Extracts were obtained through pressurized liquid extraction using water as the solvent. Bioassays were conducted during pre- and post-emergence stages by foliar spraying 15 and 30 days after sowing (DAS). The effect of extraction time (1–30 min) on inhibitory efficacy was also assessed. Chemical profiles of the extracts were characterized using high-performance liquid chromatography. The extracts significantly inhibited seed germination, with suppression rates reaching 92.7%. Plant growth was also diminished, particularly with earlier treatments (at 15 DAS), resulting in reductions of up to 32% and 53% in shoot length, and 69% and 73% in total dry mass for O. sativa L. and C. ferax, respectively. Mortality rates of O. sativa L. and C. ferax reached 64% and 58%, respectively. Phenolic compounds were identified in the extracts, and higher concentrations were observed at shorter extraction times. These findings underscore the potential of R. communis L. leaf extracts as an ecologically sustainable alternative for weed management, providing an effective and natural approach that may reduce reliance on synthetic herbicides and mitigate their environmental impact. Full article
Show Figures

Figure 1

26 pages, 11108 KiB  
Article
Warming in the Maternal Environment Alters Seed Performance and Genetic Diversity of Stylosanthes capitata, a Tropical Legume Forage
by Priscila Marlys Sá Rivas, Fernando Bonifácio-Anacleto, Ivan Schuster, Carlos Alberto Martinez and Ana Lilia Alzate-Marin
Genes 2025, 16(8), 913; https://doi.org/10.3390/genes16080913 (registering DOI) - 30 Jul 2025
Viewed by 203
Abstract
Background/Objectives: Global warming and rising CO2 concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to [...] Read more.
Background/Objectives: Global warming and rising CO2 concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to warming and elevated CO2 on progeny physiology, genetic diversity, and population structure in Stylosanthes capitata, a resilient forage legume native to Brazil. Methods: Maternal plants were cultivated under controlled treatments, including ambient conditions (control), elevated CO2 at 600 ppm (eCO2), elevated temperature at +2 °C (eTE), and their combined exposure (eTEeCO2), within a Trop-T-FACE field facility (Temperature Free-Air Controlled Enhancement and Free-Air Carbon Dioxide Enrichment). Seed traits (seeds per inflorescence, hundred-seed mass, abortion, non-viable seeds, coat color, germination at 32, 40, 71 weeks) and abnormal seedling rates were quantified. Genetic diversity metrics included the average (A) and effective (Ae) number of alleles, observed (Ho) and expected (He) heterozygosity, and inbreeding coefficient (Fis). Population structure was assessed using Principal Coordinates Analysis (PCoA), Analysis of Molecular Variance (AMOVA), number of migrants per generation (Nm), and genetic differentiation index (Fst). Two- and three-way Analysis of Variance (ANOVA) were used to evaluate factor effects. Results: Compared to control conditions, warming increased seeds per inflorescence (+46%), reduced abortion (−42.9%), non-viable seeds (−57%), and altered coat color. The germination speed index (GSI +23.5%) and germination rate (Gr +11%) improved with warming; combined treatments decreased germination time (GT −9.6%). Storage preserved germination traits, with warming enhancing performance over time and reducing abnormal seedlings (−54.5%). Conversely, elevated CO2 shortened GSI in late stages, impairing germination efficiency. Warming reduced Ae (−35%), He (−20%), and raised Fis (maternal 0.50, progeny 0.58), consistent with the species’ mixed mating system; A and Ho were unaffected. Allele frequency shifts suggested selective pressure under eTE. Warming induced slight structure in PCoA, and AMOVA detected 1% (maternal) and 9% (progeny) variation. Fst = 0.06 and Nm = 3.8 imply environmental influence without isolation. Conclusions: Warming significantly shapes seed quality, reproductive success, and genetic diversity in S. capitata. Improved reproduction and germination suggest adaptive advantages, but higher inbreeding and reduced diversity may constrain long-term resilience. The findings underscore the need for genetic monitoring and broader genetic bases in cultivars confronting environmental stressors. Full article
(This article belongs to the Special Issue Genetics and Breeding of Forage)
Show Figures

Figure 1

12 pages, 708 KiB  
Article
Techno-Functional and Nutraceutical Assessment of Unprocessed and Germinated Amaranth Flours and Hydrolysates: Impact of the Reduction of Hydrolysis Time
by Alvaro Montoya-Rodríguez, Maribel Domínguez-Rodríguez, Eslim Sugey Sandoval-Sicairos, Evelia Maria Milán-Noris, Jorge Milán-Carrillo and Ada Keila Milán-Noris
Foods 2025, 14(15), 2666; https://doi.org/10.3390/foods14152666 - 29 Jul 2025
Viewed by 187
Abstract
Amaranth is a nutritional and naturally gluten-free pseudocereal with several food applications. The germination and pepsin/pancreatin hydrolysis in amaranth releases antioxidant and anti-inflammatory compounds but the hydrolysis times (270 or 360 min) are too long to scale up in the development of amaranth [...] Read more.
Amaranth is a nutritional and naturally gluten-free pseudocereal with several food applications. The germination and pepsin/pancreatin hydrolysis in amaranth releases antioxidant and anti-inflammatory compounds but the hydrolysis times (270 or 360 min) are too long to scale up in the development of amaranth functional ingredients. The aim of this study was to estimate the influence of the germination and pepsin/pancreatin hydrolysis reduction time on the techno-functional properties and nutraceutical potential of amaranth flours and hydrolysates. The germination process increased 12.5% soluble protein (SP), 23.7% total phenolics (TPC), 259% water solubility, and 26% oil absorption in germinated amaranth flours (GAFs) compared to ungerminated amaranth flours (UAFs). The ungerminated (UAFH) and germinated (GAFH) amaranth hydrolysates showed values of degree of hydrolysis up to 50% with 150 min of sequential (pepsin + pancreatin) hydrolysis. The enzymatic hydrolysis released 1.5-fold SP and 14-fold TPC in both amaranth flours. The water solubility was higher in both hydrolysates than in their unhydrolyzed flour counterparts. The reduction in hydrolysis time did not significantly affect the nutraceutical potential of GAFH, enhancing its potential for further investigations. Finally, combining germination and enzymatic hydrolysis in amaranth enhances nutraceutical and techno-functional properties, increasing the seed. Consequently, GAF or GAFH could be used to elaborate on functional or gluten-free food products. Full article
Show Figures

Figure 1

14 pages, 888 KiB  
Article
Environmental Impact of Biodegradable Packaging Based on Chia Mucilage in Real Water Bodies
by Renata Machado Pereira da Silva, Stefanny Pereira Atanes and Sibele Santos Fernandes
Processes 2025, 13(8), 2381; https://doi.org/10.3390/pr13082381 - 27 Jul 2025
Viewed by 257
Abstract
The intense demand for alternatives to conventional plastics has increasingly motivated the development of biodegradable packaging. However, the ecological impact of these materials when discarded in natural settings has not yet been evaluated. Therefore, this study investigated the effects of films based on [...] Read more.
The intense demand for alternatives to conventional plastics has increasingly motivated the development of biodegradable packaging. However, the ecological impact of these materials when discarded in natural settings has not yet been evaluated. Therefore, this study investigated the effects of films based on chia mucilage in different aquatic environments. The solubilization time varied according to water type, ranging from 40 min in ultrapure, deionized, and distilled water to 230 min in saline water. After solubilization, all water samples exhibited increased turbidity (from 1.04 to 15.73 NTU in deionized water) and apparent color (from 0 to 44 PCU in deionized water) as well as pH variations depending on ionic strength. Deionized water also showed the highest viscosity increase (>350 Pa·s at 1 s−1). UV–Vis spectra revealed a moderate rise in absorbance between 236 and 260 nm, indicating organic compound release. Regarding phytotoxicity, the solubilized films had no toxic effect and promoted a biostimulating effect on root elongation, with Relative Germination Index values exceeding 140% in most samples. These results reinforce the potential of chia-based films for controlled disposal, particularly in low-salinity environments, while highlighting the importance of evaluating post-solubilization interactions with aquatic systems. Full article
(This article belongs to the Special Issue Advances in Waste Management and Treatment of Biodegradable Waste)
Show Figures

Figure 1

21 pages, 1208 KiB  
Review
Combination of Irreversible Electroporation and Clostridium novyi-NT Bacterial Therapy for Colorectal Liver Metastasis
by Zigeng Zhang, Guangbo Yu, Qiaoming Hou, Farideh Amirrad, Sha Webster, Surya M. Nauli, Jianhua Yu, Vahid Yaghmai, Aydin Eresen and Zhuoli Zhang
Cancers 2025, 17(15), 2477; https://doi.org/10.3390/cancers17152477 - 26 Jul 2025
Viewed by 239
Abstract
Colorectal liver metastasis (CRLM) poses a significant challenge in oncology due to its high incidence and poor prognosis in unresectable cases. Current treatments, including surgical resection, systemic chemotherapy, and liver-directed therapies, often fail to effectively target hypoxic tumor regions, which are inherently more [...] Read more.
Colorectal liver metastasis (CRLM) poses a significant challenge in oncology due to its high incidence and poor prognosis in unresectable cases. Current treatments, including surgical resection, systemic chemotherapy, and liver-directed therapies, often fail to effectively target hypoxic tumor regions, which are inherently more resistant to these interventions. This review examines the potential of a novel therapeutic strategy combining irreversible electroporation (IRE) ablation and Clostridium novyi-nontoxic (C. novyi-NT) bacterial therapy. IRE is a non-thermal tumor ablation technique that uses high-voltage electric pulses to create permanent nanopores in cell membranes, leading to cell death while preserving surrounding structures, and is often associated with temporary tumor hypoxia due to disrupted perfusion. C. novyi-NT is an attenuated, anaerobic bacterium engineered to selectively germinate and proliferate in hypoxic tumor regions, resulting in localized tumor cell lysis while sparing healthy, oxygenated tissue. The synergy between IRE-induced hypoxia and hypoxia-sensitive C. novyi-NT may enhance tumor destruction and stimulate systemic antitumor immunity. Furthermore, the integration of advanced imaging and artificial intelligence can support precise treatment planning and real-time monitoring. This integrated approach holds promise for improving outcomes in patients with CRLM, though further preclinical and clinical validation is needed. Full article
(This article belongs to the Section Cancer Metastasis)
Show Figures

Figure 1

25 pages, 4453 KiB  
Article
Regulatory Mechanisms of Exogenous Gibberellin on Seed Germination and Transcriptomic Responses in Lomatogonium rotatum
by Kefan Cao, Yingtong Mu, Sihai Lu and Yanyan Zhao
Genes 2025, 16(8), 878; https://doi.org/10.3390/genes16080878 - 26 Jul 2025
Viewed by 234
Abstract
Gibberellins (GAs) are essential phytohormones that regulate seed dormancy release and germination. Lomatogonium rotatum (L.) Fries ex Nym is a traditional medicinal plant whose seed germination is often hindered by physiological dormancy. In this study, we systematically investigated the effects of exogenous GA [...] Read more.
Gibberellins (GAs) are essential phytohormones that regulate seed dormancy release and germination. Lomatogonium rotatum (L.) Fries ex Nym is a traditional medicinal plant whose seed germination is often hindered by physiological dormancy. In this study, we systematically investigated the effects of exogenous GA3 on the seed germination of L. rotatum and elucidated the underlying molecular regulatory mechanisms via transcriptomic analysis. GA3 treatment (500 mg/L for 24 h) significantly improved the germination rate, vigor index, and other germination traits. RNA-seq analysis identified time-dependent transcriptional changes in GA3-treated seeds across three developmental stages (24 h, 72 h, and 96 h). KEGG enrichment and K-means clustering revealed dynamic actiSvation of hormonal signaling, secondary metabolism, and DNA replication pathways. WGCNA uncovered two hormone-responsive co-expression modules (Red and Lightcyan) corresponding to early and late stages of germination, respectively. Key genes related to ABA and GA biosynthesis and signal transduction showed phase-specific expression, highlighting the coordinated hormonal regulation during seed germination. Our findings provide new insights into the molecular basis of GA3-regulated seed germination and offer theoretical support for the cultivation and utilization of L. rotatum. Full article
(This article belongs to the Section Plant Genetics and Genomics)
Show Figures

Figure 1

24 pages, 1725 KiB  
Article
Effects of Germination on the Nutritional Profile of Five Distinct Pea Varieties
by Hojjat Abdollahikhamene, Shirin Kazemzadeh Pournaki and Clifford Hall
Molecules 2025, 30(15), 3114; https://doi.org/10.3390/molecules30153114 - 25 Jul 2025
Viewed by 270
Abstract
The effects of germination on pea composition have been established. However, the effects of germination on the nutritional profile of different pea varieties have not been extensively reported. Therefore, five varieties (Passion, Greenwood, Durwood, Agassiz, and Treasure) of peas were germinated for up [...] Read more.
The effects of germination on pea composition have been established. However, the effects of germination on the nutritional profile of different pea varieties have not been extensively reported. Therefore, five varieties (Passion, Greenwood, Durwood, Agassiz, and Treasure) of peas were germinated for up to six days, and their nutrient profiles, protein digestibility, in vitro protein digestibility corrected amino acid score (IV-PDCAAS), and antioxidant activity (DPPH) were determined. In addition, B vitamins were determined for the first time in most of the varieties evaluated. Germination enhanced protein digestibility across all varieties, whereas IV-PDCAAS tended to decrease with increasing germination day. The impact of germination on starch content varied, with decreasing percentages found in some varieties and increased percentages found in others. Soluble fiber increased and insoluble fiber decreased with increasing germination days. Thiamine and niacin tended to increase with increasing germination day, while pyridoxine and folate decreased. The radical scavenging activity of the germinated peas increased with increasing germination days. Overall, germination tended to improve the nutritional composition of peas, with only a few exceptions. Furthermore, the interaction effects between variety and germination day support the importance of knowing both variety and length of germination when creating germinated pea products. Full article
Show Figures

Figure 1

12 pages, 1597 KiB  
Article
Effects of Anthropogenic Vibratory Noise on Plant Development and Herbivory
by Estefania Velilla, Laura Bellato, Eleanor Collinson and Wouter Halfwerk
Acoustics 2025, 7(3), 45; https://doi.org/10.3390/acoustics7030045 - 25 Jul 2025
Viewed by 234
Abstract
Anthropogenic infrastructure, such as inland wind turbines commonly found in agricultural fields, has substantially increased subterranean vibratory noise in the past decades. Plants, being rooted in soil, are continuously exposed to these vibrations, yet we have little understanding of how vibrational noise affects [...] Read more.
Anthropogenic infrastructure, such as inland wind turbines commonly found in agricultural fields, has substantially increased subterranean vibratory noise in the past decades. Plants, being rooted in soil, are continuously exposed to these vibrations, yet we have little understanding of how vibrational noise affects plant development and, consequently, plant–insect interactions. Here, we examine the impact of windmill-like vibrational noise on the growth of Pisum sativum and its full-factorial interaction with the generalist herbivore Spodoptera exigua. Plants were exposed to either high or low vibrational noise from seed germination to the seed production stage. We recorded germination, flowering, fruiting time, and daily shoot length. Additionally, we measured herbivory intensity by Spodoptera exigua caterpillars placed on a subset of plants. Plants exposed to high vibrational noise grew significantly faster and taller than those in the low-noise treatment. Additionally, we found a marginally significant trend for earlier flowering in plants exposed to high noise. We did not find a significant effect of vibrational noise on herbivory. Our results suggest that underground vibrational noise can influence plant growth rates, which may potentially have ecological and agricultural implications. Faster growth may alter interspecific competition and shift trade-offs between growth and defense. Understanding these effects is important in assessing the broader ecological consequences of renewable energy infrastructure. Full article
Show Figures

Figure 1

12 pages, 1798 KiB  
Article
Protective Efficacy Induced by Virus-like Particles Expressing Dense Granule Protein 5 of Toxoplasma gondii
by Su In Heo, Hae-Ji Kang, Jie Mao, Zhao-Shou Yang, Md Atique Ahmed and Fu-Shi Quan
Vaccines 2025, 13(8), 787; https://doi.org/10.3390/vaccines13080787 - 24 Jul 2025
Viewed by 357
Abstract
Background: Toxoplasma gondii (T. gondii) causes severe disease in immunocompromised individuals and pregnant women, underscoring the urgent need for effective vaccines against toxoplasmosis. The dense granule protein 5 (GRA5) of T. gondii plays a key role in parasitic cyst formation. [...] Read more.
Background: Toxoplasma gondii (T. gondii) causes severe disease in immunocompromised individuals and pregnant women, underscoring the urgent need for effective vaccines against toxoplasmosis. The dense granule protein 5 (GRA5) of T. gondii plays a key role in parasitic cyst formation. Methods: This study evaluated the protective immune responses induced by a virus-like particle (VLP) vaccine expressing the T. gondii-derived antigen GRA5 in a mouse model challenged with the ME49 strain of T. gondii. GRA5 VLPs were generated using a baculovirus expression system, and VLP formation was confirmed by Western blotting and visualized using transmission electron microscopy. Mice were intranasally immunized with GRA5 VLPs three times at 4-week intervals to induce immune responses, followed by infection with T. gondii ME49. Results: Intranasal immunization with GRA5 VLPs induced parasite-specific IgG antibody responses in the serum and both IgG and IgA antibody responses in the brain. Compared to the non-immunized group, immunized mice exhibited significantly higher levels of germinal center B cells and antibody-secreting cell responses. Moreover, the VLP vaccine suppressed the production of IFN-γ and IL-6 cytokines, leading to a significant reduction in brain inflammation and decreased cyst counts following lethal challenge with T. gondii ME49 infection. Conclusion: These findings suggest that the GRA5 VLP vaccine derived from T. gondii elicits a protective immune response, highlighting its potential as an effective vaccine candidate against toxoplasmosis. Full article
(This article belongs to the Special Issue Research on Immune Response and Vaccines: 2nd Edition)
Show Figures

Figure 1

17 pages, 4216 KiB  
Article
Sugarcane Phenology Retrieval in Heterogeneous Agricultural Landscapes Based on Spatiotemporal Fusion Remote Sensing Data
by Yingpin Yang, Zhifeng Wu, Dakang Wang, Cong Wang, Xiankun Yang, Yibo Wang, Jinnian Wang, Qiting Huang, Lu Hou, Zongbin Wang and Xu Chang
Agriculture 2025, 15(15), 1578; https://doi.org/10.3390/agriculture15151578 - 23 Jul 2025
Viewed by 226
Abstract
Accurate phenological information on sugarcane is crucial for guiding precise cultivation management and enhancing sugar production. Remote sensing offers an efficient approach for large-scale phenology retrieval, but most studies have primarily focused on staple crops. The methods for retrieving the sugarcane phenology—the germination, [...] Read more.
Accurate phenological information on sugarcane is crucial for guiding precise cultivation management and enhancing sugar production. Remote sensing offers an efficient approach for large-scale phenology retrieval, but most studies have primarily focused on staple crops. The methods for retrieving the sugarcane phenology—the germination, tillering, elongation, and maturity stages—remain underexplored. This study addresses the challenge of accurately monitoring the sugarcane phenology in complex terrains by proposing an optimized strategy integrating spatiotemporal fusion data. Ground-based validation showed that the change detection method based on the Double-Logistic curve significantly outperformed the threshold-based approach, with the highest accuracy for the elongation and maturity stages achieved at the maximum slope points of the ascending and descending phases, respectively. For the germination and tillering stages with low canopy cover, a novel time-windowed change detection method was introduced, using the first local maximum of the third derivative curve (denoted as Point A) to establish a temporal buffer. The optimal retrieval models were identified as 25 days before and 20 days after Point A for germination and tillering, respectively. Among the six commonly used vegetation indices, the NDVI (normalized difference vegetation index) performed the best across all the phenological stages. Spatiotemporal fusion using the ESTARFM (Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model) significantly improved the monitoring accuracy in heterogeneous agricultural landscapes, reducing the RMSE (root-mean-squared error) by 21–46%, with retrieval errors decreasing from 18.25 to 12.97 days for germination, from 8.19 to 4.41 days for tillering, from 19.17 to 10.78 days for elongation, and from 19.02 to 15.04 days for maturity, highlighting its superior accuracy. The findings provide a reliable technical solution for precision sugarcane management in heterogeneous landscapes. Full article
Show Figures

Figure 1

25 pages, 4661 KiB  
Article
Detection of Organophosphorus, Pyrethroid, and Carbamate Pesticides in Tomato Peels: A Spectroscopic Study
by Acela López-Benítez, Alfredo Guevara-Lara, Diana Palma-Ramírez, Karen A. Neri-Espinoza, Rebeca Silva-Rodrigo and José A. Andraca-Adame
Foods 2025, 14(14), 2543; https://doi.org/10.3390/foods14142543 - 21 Jul 2025
Viewed by 243
Abstract
Tomatoes are among the most widely consumed and economically significant fruits in the world. However, the extensive use of pesticides in their cultivation has led to the contamination of the peels, posing potential health risks to consumers. As one of the top global [...] Read more.
Tomatoes are among the most widely consumed and economically significant fruits in the world. However, the extensive use of pesticides in their cultivation has led to the contamination of the peels, posing potential health risks to consumers. As one of the top global producers, consumers, and exporters of tomatoes, Mexico requires rapid, non-destructive, and real-time methods for pesticide monitoring. In this study, a detailed characterization of six pesticides using Raman and Fourier Transform Infrared (FT-IR) spectroscopies was carried out to identify their characteristic vibrational modes. The pesticides examined included different chemical classes commonly used in tomato cultivation: organophosphorus (dichlorvos and methamidophos), pyrethroids (lambda-cyhalothrin and cypermethrin), and carbamates (methomyl and benomyl). Tomato peel samples were examined both before and after pesticide application. Prior to treatment, the peel exhibited a well-organized polygonal structure and showed the presence of carotenoid compounds. After pesticide application, no visible structural damage was observed; however, distinct vibrational bands enabled the detection of each pesticide. Organophosphorus pesticides could be identified through vibrational bands associated with P-O and C-S bonds. Pyrethroid detection was facilitated by benzene ring breathing modes and C=C stretching vibrations, while carbamates were identified through C-N stretching contributions. Phytotoxicity testing in the presence of pesticides indicates no significant damage during the germination of tomatoes. Full article
Show Figures

Figure 1

22 pages, 1446 KiB  
Review
Integrating Redox Proteomics and Computational Modeling to Decipher Thiol-Based Oxidative Post-Translational Modifications (oxiPTMs) in Plant Stress Physiology
by Cengiz Kaya and Francisco J. Corpas
Int. J. Mol. Sci. 2025, 26(14), 6925; https://doi.org/10.3390/ijms26146925 - 18 Jul 2025
Viewed by 273
Abstract
Redox signaling is central to plant adaptation, influencing metabolic regulation, stress responses, and developmental processes through thiol-based oxidative post-translational modifications (oxiPTMs) of redox-sensitive proteins. These modifications, particularly those involving cysteine (Cys) residues, act as molecular switches that alter protein function, structure, and interactions. [...] Read more.
Redox signaling is central to plant adaptation, influencing metabolic regulation, stress responses, and developmental processes through thiol-based oxidative post-translational modifications (oxiPTMs) of redox-sensitive proteins. These modifications, particularly those involving cysteine (Cys) residues, act as molecular switches that alter protein function, structure, and interactions. Advances in mass spectrometry-based redox proteomics have greatly enhanced the identification and quantification of oxiPTMs, enabling a more refined understanding of redox dynamics in plant cells. In parallel, the emergence of computational modeling, artificial intelligence (AI), and machine learning (ML) has revolutionized the ability to predict redox-sensitive residues and characterize redox-dependent signaling networks. This review provides a comprehensive synthesis of methodological advancements in redox proteomics, including enrichment strategies, quantification techniques, and real-time redox sensing technologies. It also explores the integration of computational tools for predicting S-nitrosation, sulfenylation, S-glutathionylation, persulfidation, and disulfide bond formation, highlighting key models such as CysQuant, BiGRUD-SA, DLF-Sul, and Plant PTM Viewer. Furthermore, the functional significance of redox modifications is examined in plant development, seed germination, fruit ripening, and pathogen responses. By bridging experimental proteomics with AI-driven prediction platforms, this review underscores the future potential of integrated redox systems biology and emphasizes the importance of validating computational predictions, through experimental proteomics, for enhancing crop resilience, metabolic efficiency, and precision agriculture under climate variability. Full article
(This article belongs to the Section Molecular Plant Sciences)
Show Figures

Figure 1

18 pages, 1182 KiB  
Article
Effects of Remote Barley Seed Treatment with Weak Non-Thermal Pulsed Electromagnetic Fields on Plant Development and Yields
by Igor F. Turkanov, Elena V. Bondarchuk, Valery G. Gryaznov, Ekaterina A. Galkina, Alexey Yu. Guzenko, Vladimir G. Zainullin, Elena G. Kozar and Irina M. Kaigorodova
Seeds 2025, 4(3), 35; https://doi.org/10.3390/seeds4030035 - 18 Jul 2025
Viewed by 319
Abstract
Numerous scientific studies have confirmed the effectiveness of seed bioactivation using electromagnetic fields (EMFs) in agriculture. This article presents the results of the remote application of an EMF TOR device in the cultivation of barley Hordeum vulgare L. Laboratory studies and field tests [...] Read more.
Numerous scientific studies have confirmed the effectiveness of seed bioactivation using electromagnetic fields (EMFs) in agriculture. This article presents the results of the remote application of an EMF TOR device in the cultivation of barley Hordeum vulgare L. Laboratory studies and field tests were conducted, showing a positive effect on the growth and development of plants both when treating dry seeds before sowing and when treating sown seeds in the field. The optimal time period for EMF treatment was determined: treating air-dried seeds with EMFs before sowing for 10–15 min increased germination by 5–18% and the growth rate of seedlings by 2–3 times. The maximum observed effect occurred during the treatment period from 7:00 to 11:00. As a result of changing the balance of phytohormones, the further stimulation of the root system and the assimilation surface of plants was noted due to a 1.5-fold increase in the content of auxins. The density of productive stems, ear length, seed set, and 1000 seed weight increased, which ultimately led to an increase in yield by more than 10% and, in some varieties, to a decrease in the protein content in grains compared to the control variant (by 3–22%), bringing them closer to brewing conditions. Full article
Show Figures

Figure 1

13 pages, 1279 KiB  
Article
Transcriptome Sequencing-Based Analysis of Premature Fruiting in Amomum villosum Lour.
by Yating Zhu, Shuang Li, Hongyou Zhao, Qianxia Li, Yanfang Wang, Chunyong Yang, Ge Li, Yanqian Wang and Lixia Zhang
Biology 2025, 14(7), 883; https://doi.org/10.3390/biology14070883 - 18 Jul 2025
Viewed by 280
Abstract
Amomum villosum Lour., a perennial medicinal plant in the Zingiber genus, usually requires approximately 3–4 years of vegetative growth from seed germination to first fruiting, resulting in high initial investment costs and a prolonged revenue cycle, which pose significant challenges to the industry’s [...] Read more.
Amomum villosum Lour., a perennial medicinal plant in the Zingiber genus, usually requires approximately 3–4 years of vegetative growth from seed germination to first fruiting, resulting in high initial investment costs and a prolonged revenue cycle, which pose significant challenges to the industry’s sustainable development. Our research team observed a distinct premature fruiting phenomenon in A. villosum. We investigated the regulatory mechanisms underlying premature fruiting in A. villosum by identifying the key differentially expressed genes (DEGs) and metabolic pathways governing the premature fruiting (Precocious) and typical plants (CK) of the ‘Yunsha No.8’ cultivar. Transcriptomic sequencing (RNA-seq) and bioinformatic analyses were performed using the DNBSEQTM platform. The sequencing generated 29.0 gigabases (Gb) of clean data, and 115,965 unigenes were identified, with an average length of 1368 bp. Based on the sequencing results, 1545 DEGs were identified. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were annotated for these DEGs. This study identifies phytohormone signaling, carbohydrate and lipid metabolism, and polysaccharide degradation as critical pathways controlling premature fruiting in A. villosum. Six randomly selected DEGs were validated using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and the results corroborated the transcriptome data, confirming their reliability. This study lays the foundation for the elucidation of the molecular mechanisms and metabolic pathways driving premature fruiting in A. villosum. Full article
(This article belongs to the Special Issue Young Investigators in Biochemistry and Molecular Biology)
Show Figures

Figure 1

Back to TopTop