Search Results (737)

This paper presents the design of a seed-pressing mechanism for a high-speed suction-type precision peanut planter to address the issue of poor seeding performance at high travel speeds and to reduce seed bounce within furrows. To clarify the working principle of the mechanism, a force analysis of peanut seeds in the furrow and a numerical study using discrete element analysis were conducted under high-speed operating conditions. Simulation results show that when the distance between the center of the seed-pressing wheel and the seeding-tube outlet (DCSPW-STO) is 146.11 mm, the seed-pressing wheel diameter is 198.13 mm, and the machine operating velocity is 6.45 km h⁻¹, the plant spacing qualification index and seeding depth compliance index for peanuts planted after rolling reach their maximum values. The corresponding germination rates of 93.78% and 90.65% indicate satisfactory sowing performance. Field validation trials demonstrate that when DCSPW-STO (l_fz) is 146 mm, the seed-pressing wheel diameter (d_fz) is 198 mm, and the machine operating velocity (v) is 6.45 km h⁻¹, the post-seeding plant-spacing qualification index and the seeding-depth compliance index reach 90.31% and 89.18%, respectively. Although slightly lower than the simulation results, these values meet the operational requirements for peanut seeding. Field performance comparisons with non-pressure seeding units further confirm that units equipped with the seed-pressing and soil-covering mechanisms significantly improve both the plant-spacing qualification index and the seeding-depth compliance index, satisfying agronomic requirements for high-speed peanut cultivation. Full article

Research on the remediation of hydrocarbon contaminated peatlands is limited; in particular, hydrocarbon effects on seed germination is critical for effective reclamation. This study examined germination responses of six wetland plant species under greenhouse and laboratory conditions. Seeds were exposed to hydrocarbon-contaminated peat soil and ground water under two light treatments (light, total darkness) for four weeks. Species specific responses in seed germination and germination velocity occurred under different light conditions and exposure to hydrocarbon-contaminated peat soil and water. Light significantly impacted germination, while hydrocarbon-contaminated peat soil and water had no effect. Glyceria grandis (83.5%) and Scirpus microcarpus (74%) had significantly higher germination rates even in contaminated treatments than Carex aquatilis (28%) and Typha latifolia (38%), which had modest germination. Modified Timson’s Index (germination velocity) was significantly greater in Scirpus microcarpus (21.90) and Glyceria grandis (19.20) than in other species after 30 days. Carex utriculata and Scirpus validus had ≤0.5% germination and ≤0.2 velocity. The overall species mean germination time was >9 days with a low (≤0.7) germination index. Ordination using several germination variables separated some species. These findings suggest Scirpus microcarpus and Glyceria grandis have high tolerance to hydrocarbon contamination and may be effective candidates for the phytoremediation and restoration of contaminated peatlands. Full article

Water deficit is a limitation to maize (Zea mays L.) productivity, and seed physiological conditioning (priming) is a strategy to mitigate its effects. Niobium (Nb), an abundant element in the Earth’s crust and crucial for emerging technologies, is primarily produced and exported by Brazil, particularly in the state of Minas Gerais. However, its behavior in soil and effects on plants remain poorly understood. This study evaluated the impact of maize seed hydropriming with different solutions, including ammonium niobate (V) oxalate (C₄H₄NNbO₉), on germination, seedling physiological performance under water deficit, and recovery after rehydration. The experiment was conducted in a greenhouse using a randomized block design with eight treatments and five replications. The germination speed index (GSI) was recorded over a period of seven days. Water deficit was imposed on day eight by suspending irrigation for seven days, followed by rehydration until day 21. Morphological, biochemical, and physiological traits were evaluated, including biomass, pigments, oxidative stress, antioxidant activity, starch, and osmolytes, with photosynthetic parameters measured during rehydration. Hydropriming with Nb-enhanced seed germination but also induced oxidative stress and reduced biomass accumulation. Nb seed priming affected photosynthetic performance in a treatment-dependent manner, leading to phototoxic effects. Overall, although Nb shows biostimulant potential by improving maize germination, its use under water-deficit conditions may trigger toxic responses associated with increased oxidative stress and growth inhibition. These findings highlight the need for further studies to define safe and effective Nb concentrations for improving drought tolerance. Full article

Low-temperature stress severely restricts cotton seed germination and seedling establishment, especially in early spring. Ascorbic acid (AsA) priming is a promising strategy to enhance stress tolerance, yet its mechanisms in cotton remain unclear. This study examined the effects of AsA priming on seed germination at 15 °C. Seeds were treated with 0, 25, 50, or 100 mg/L AsA for 3, 6, 12, or 24 h. Results showed that 50 mg/L AsA for 24 h significantly improved germination potential, rate, index, and promptness index (p < 0.05). Compared with water-primed seeds, AsA-primed seeds exhibited greater radicle length (+17.67%) and fresh weight (+136.26%) under chilling stress. This treatment markedly increased antioxidant enzyme activities, including POD (+196.74%), SOD (+43.81%), and CAT (+49.43%), while also promoting the accumulation of Ascorbate–Glutathione cycle-related enzymes and metabolites, thereby reinforcing the antioxidant defense system. Multidimensional statistical analyses further indicated that AsA enhanced root growth by stimulating antioxidant defenses while inducing a trade-off that slightly reduced fresh weight, suggesting a balance between growth and oxidative protection. Overall, AsA priming improves cotton seed cold tolerance by activating enzymatic and non-enzymatic antioxidant systems and mediating a growth–defense trade-off, underscoring its potential as an effective priming agent for early sowing under low-temperature stress. Full article

Apples and tomatoes are among the most consumed products all over the world, as well as the natural juices prepared from each of them. The large quantities of resulting by-products should be reused in various directions within the circular economy. In this study, apple and tomato pomaces were tested as potential biofertilizers for agricultural crops. To this end, aqueous extracts of apple pomace and tomato pomace were prepared in two concentrations (0.05% and 0.5%) and used to treat wheat caryopses and sprouts. The following were evaluated: mitotic index, genotoxic index, caryopses germination rate, and wheat sprout growth. The biotic response of wheat to treatments with the apple and tomato pomace extracts consisted of reduced mitotic activity, i.e., cytotoxicity, and the formation of genetic abnormalities, i.e., genotoxicity. The cytotoxicity and the genotoxicity were reflected at the macro level in phytotoxic effects, manifested by a reduction in the germination rate of caryopses and a decrease in the length of wheat roots and shoots. Physiological parameters were positively correlated with the mitotic index and negatively correlated with the genotoxic index. The obtained results point us not to recommend the use of unprocessed apple and tomato pomaces as biofertilizers, but, on the contrary, as bioherbicides. Full article

Human herpesvirus-8 (HHV-8)-associated multicentric Castleman disease (MCD) is a rare lymphoproliferative disorder with systemic and cutaneous manifestations that can be diagnostically challenging, especially in immunocompromised patients. We report the case of a 68-year-old man with HIV and biopsy-proven Kaposi sarcoma (KS), who developed progressive fevers, night sweats, weight loss, and fatigue, accompanied by diffuse lymphadenopathy, splenomegaly, and new erythematous and hyperpigmented lesions shortly after intravenous immunoglobulin therapy for Guillain–Barré syndrome. A laboratory evaluation revealed that the patient had elevated total protein and polyclonal hypergammaglobulinemia, without monoclonality. Imaging demonstrated widespread lymphadenopathy and splenomegaly. A core lymph node biopsy showed polytypic plasmacytosis, but was non-diagnostic. Given the ongoing symptoms, an excisional biopsy was performed, revealing regressed germinal centers with increased interfollicular vascularity, mantle zone “onion skinning,” and HHV-8 LANA-1 nuclear positivity, establishing the diagnosis of HHV-8-associated MCD. Rituximab monotherapy was initiated, resulting in clinical improvement, resolution of the constitutional symptoms, and stabilization of ascites. This case highlights the importance of maintaining a high index of suspicion for MCD in patients with KS who develop new systemic or cutaneous findings, the limitations of a core biopsy, and the value of a timely excisional biopsy in guiding diagnosis and treatment. Full article

Sustainable manure recycling in cold climates faces low efficiency and nutrient loss. This study evaluated housefly larva-pretreated manure (HL) for winter swine manure composting in East China, comparing it to sawdust-conditioned (CK2) and untreated manure (CK1). Larval pretreatment converted 12.71% of manure weight into biomass, assimilating 10.69% C, 30.55% N, 8.54% P, and 11.53% K. Harvested larvae contained 53.35% crude protein, with amino acids matching/exceeding fishmeal and soybean meal, while heavy metals were below safety limits. Theoretical annual larval protein yield per unit area (29,530 kg·mu⁻¹·year⁻¹) was 206.5 times higher than soybean crops. During composting, the HL treatment promoted early protease and catalase activation. This enzymatic synergy accelerated organic matter degradation and maturation, achieving a germination index of 147.67% by day 51. Coordinated nitrate and nitrite reductase activity in HL facilitated efficient denitrification, minimizing NO₂⁻ accumulation and N₂O emissions. Consequently, HL composting achieved faster stabilization, enhanced nutrient retention, and greater protein recovery compared to controls. These findings demonstrate that housefly larval pretreatment offers a climate-resilient and scalable strategy for winter manure management and protein valorization, with strong potential for applications in cold and resource-limited agricultural systems worldwide. Full article

In this study, the effects of composting phase and bulking agent on macronutrient extraction and the chemical, physicochemical, and biological properties of 20 compost teas from poultry waste composting mixtures were evaluated. Phosphorus (P) extraction was more efficient during stabilization after the thermophilic phase; however, water-soluble P declined as composting progressed. K was more amenable to extraction, with yields ranging from 30% to 70%, followed by N (2% to 12%) and P (1% to 7%). Compost tea quality was clearly affected by both the bulking agent and the composting stage. Bulking agents that accelerate the process, such as cotton waste (CW) and Napier grass (NG), contributed to nutrient mineralization, increasing availability in the compost tea but also raising salt contents responsible for phytotoxicity. In contrast, tree trimmings (TT), sawdust (S), and sugarcane bagasse (SCB) showed better results, striking a balance between nutrient availability and salt content. The period between the thermophilic phase and cooling was the most suitable for extraction, providing the greatest contribution of water-soluble nutrients. This study highlights the influence of bulking agents and composting phases on nutrient extraction and phytotoxicity of compost teas and provides new insights into the role of electrical conductivity as a threshold indicator for safe agricultural application. Full article

The growing demand for sustainable waste management practices has prompted interest in the land application of paper sludge as an alternative to landfilling and incineration. This study evaluates the environmental potential of paper sludge derived from recycled hygienic paper production by investigating its effects on soil respiration, seed germination, and seedling development. A comprehensive set of respirometric tests using the OxiTop^® system assessed microbial activity in soil amended with various concentrations of paper sludge (1–100%). Concurrently, bioassays using Lepidium sativum L. and Pisum sativum L. seeds examined the phytotoxicity and physiological response during germination. The results show that low to moderate sludge concentrations (1–20%) stimulated microbial activity and enhanced germination parameters, with a germination index (GI) up to 150% at 1%. However, higher concentrations (>40%) led to oxygen depletion, microbial stress, and decreased plant growth, indicating potential phytotoxicity and the need for application thresholds. For certain intermediate concentrations (e.g., 30–40%), a delay of approximately 21 days before sowing is recommended to allow microbial communities to stabilize and avoid initial stress conditions for plants. This study demonstrates that controlled application of paper sludge in soil systems can serve as a viable and sustainable disposal method, supporting circular economy principles and reducing the environmental burden of paper industry by-products. Full article

This study reports on green-synthesized zinc oxide nanoparticles (ZnONPs), focusing on their physicochemical characterization, photocatalytic properties, and agricultural applications. Dynamic light scattering (DLS) analysis revealed a mean hydrodynamic diameter of 337.3 nm and a polydispersity index (PDI) of 0.400, indicating moderate polydispersity and nanoparticle aggregation, typical of biologically synthesized systems. High-resolution transmission electron microscopy (HR-TEM) showed predominantly spherical particles with an average diameter of ~28 nm, exhibiting slight agglomeration. Energy-dispersive X-ray spectroscopy (EDX) confirmed the elemental composition of zinc and oxygen, while X-ray diffraction (XRD) analysis identified a hexagonal wurtzite crystal structure with a dominant (002) plane and an average crystallite size of ~29 nm. Photoluminescence (PL) spectroscopy displayed a distinct near-band-edge emission at ~462 nm and a broad blue–green emission band (430–600 nm) with relatively low intensity. The ultraviolet–visible spectroscopy (UV–Vis) absorption spectrum of the synthesized ZnONPs exhibited a strong absorption peak at 372 nm, and the optical band gap was calculated as 2.67 eV using the Tauc method. Fourier-transform infrared spectroscopy (FTIR) analysis revealed both similarities and distinct differences to the pigeon extract, confirming the successful formation of nanoparticles. A prominent absorption band observed at 455 cm⁻¹ was assigned to Zn–O stretching vibrations. X-ray photoelectron spectroscopy (XPS) analysis showed that raw pigeon droppings contained no Zn signals, while their extract provided organic biomolecules for reduction and stabilization, and it confirmed Zn²⁺ species and Zn–O bonding in the synthesized ZnONPs. Photocatalytic degradation assays demonstrated the efficient removal of pollutants from sewage water, leading to significant reductions in total dissolved solids (TDS), chemical oxygen demand (COD), and total suspended solids (TSS). These results are consistent with reported values for ZnO-based photocatalytic systems, which achieve biochemical oxygen demand (BOD) levels below 2 mg/L and COD values around 11.8 mg/L. Subsequent reuse of treated water for irrigation yielded promising agronomic outcomes. Wheat and barley seeds exhibited 100% germination rates with ZnO NP-treated water, which were markedly higher than those obtained using chlorine-treated effluent (65–68%) and even the control (89–91%). After 21 days, root and shoot lengths under ZnO NP irrigation exceeded those of the control group by 30–50%, indicating enhanced seedling vigor. These findings demonstrate that biosynthesized ZnONPs represent a sustainable and multifunctional solution for wastewater remediation and agricultural enhancement, positioning them as a promising candidate for integration into green technologies that support sustainable urban development. Full article

Biostimulants based on Bradyrhizobium japonicum are commonly used in soybean production. However, the effect of nitrogen-fixing bacteria in consortia with other plant growth-promoting rhizobacteria (PGPR) and their integration with mineral nutrients on soybean seed quality has not been explored. The study aimed to examine the effects of five treatments on seed germination and initial seedling growth of two soybean cultivars (‘NS Apolo’, ‘NS Rubin’): control (untreated seeds); Br. japonicum (BJ), BJ and nutrient complex (NC), BJ, Azotobacter chroococcum (AC), Bacillus subtilis (BS), and NC; BJ, AC, Bacillus megaterium (BM), and NC. Seed treatments significantly enhanced germination energy, seedling vigor index, root length, fresh shoot weight, fresh root weight, dry shoot weight, and dry root weight of both cultivars, as well as final germination, shoot length, and shoot elongation rate of ‘NS Rubin’, as compared to the control. The highest effect on the investigated parameters was achieved by integrated use of PGPR and nutrients (BJ + BM + AC + NC), indicating that integration of PGPR with a targeted NC represents an innovative approach with practical implications for improving early soybean establishment and field performance. Full article

Beta-glucans (β-glucans), polysaccharides found in cereals and fungi, are recognized for their prebiotic and potential anti-cancer activities, particularly in the colorectal area. This study aims to optimize the production of β-glucan through the solid-state fermentation of germinated Riceberry rice with Pleurotus ostreatus and evaluate the bioactivities of the resulting extract. The crude β-glucan extract, obtained with a recovery rate of 54.95% and 79.98% purity, demonstrated an effective extraction process, as confirmed by thermogravimetric analysis (TGA). Fourier-transform infrared spectroscopy (FTIR) analysis verified the presence of β-1,3/1,6-glycosidic linkages, characteristic of the bioactive β-glucans found in yeast and mushrooms. The biological assessment demonstrated the extract’s functional properties. At a concentration of 1 mg/mL, the crude β-glucan extract significantly promoted the growth of probiotics Lacticaseibacillus rhamnosus and Bacillus coagulans, exhibiting high Prebiotic Index (PI) values of 6.36 ± 0.72 and 115.70 ± 10.19, respectively, with PI values indicating strong prebiotic potential. For comparison, the standard prebiotic inulin yielded PI values of 0.41 ± 0.09 and 90.53 ± 2.28 for the same respective bacteria, highlighting the superior performance of the fungal-fermented β-glucan. Furthermore, the extract displayed efficacy in inhibiting colon cancer cells in preliminary in vitro tests. It reduced the viability of the SW480 colorectal cancer cell line by 66.23% and induced cell death in 27.94 ± 0.93% of the cells after 48 h of treatment, performing comparably to a commercial yeast β-glucan standard. Crucially, the extract showed no significant cytotoxicity toward the normal human colon cell line, CCD-841 CoN. These findings highlight the promising method of fungal solid-state fermentation on germinated Riceberry rice in the production of high-purity, bioactive β-glucans for use in functional foods. Full article

Sustainable development requires implementation of eco-friendly practices and a circular approach in both agricultural and industrial systems. This study evaluated winery flotation wastewater (WFW) as a cultivation substrate for Bacillus sp. 10/R isolated from grapevine rhizosphere for sustainable biostimulant production. The bacterial isolate was characterized by 16S rRNA sequencing and biochemical tests, showing the highest similarity with Bacillus mojavensis and Bacillus halotolerans. Plant growth-promoting traits were assessed via assays for hydrolytic enzymes, ACC (1-aminocyclopropane-1-carboxylate) deaminase, and IAA (indole acetic acid) production, as well as for phosphate solubilization. The isolate was cultivated in WFW, including monitoring of biomass growth, enzymatic activity, and substrate composition changes. The resulting cultivation broths based on WFW (WFW-CB) and nutrient broth (NB-CB) were tested as barley seed treatment at five dosages, using sterile media and water as controls. The results have displayed strong pectinase (EAI–enzyme activity index 2.79) and cellulase activity (2.33), moderate xylanase (1.75) and ACC deaminase activity (growth zone 54.67 ± 0.58 mm), and moderate IAA production (9.66 µg/mL). Biomass content has increased by two log units within 48 h (up to 9.06 log CFU/mL), with stable pectinase activity (~2.2 U/mL). Germination assays revealed that 10% WFW-CB and 50% WFW enhanced germination indices and biomass, whereas undiluted WFW and WFW-CB inhibited germination. These results indicate that WFW is a suitable substrate for Bacillus sp. 10/R cultivation, linking industrial wastewater valorization with plant biostimulant production in a circular economy framework. Full article

Petroleum pollution can pose a serious threat to soil health and its ecological functions. This study investigated the efficacy of bacterial treatments for bioremediation of diesel-contaminated soil under outdoor conditions for a period of 4 months. Unlike most previous studies conducted under laboratory conditions, this study applied single and multi-bacterial consortia directly into diesel-contaminated soil under outdoor conditions, evaluating both hydrocarbon degradation and soil toxicity changes. Three treatments using a single strain, a 4-strain consortium, and an 8-strain consortium were applied to 2% (v/w) diesel-contaminated soil, and their performance was compared to uncontaminated and untreated controls. Total petroleum hydrocarbon (TPH) degradation was quantified using GC-FID, and the soil toxicity was assessed using Eisenia fetida toxicity test and higher plant germination assays. As the experiment demonstrated, the multi-strain bacterial consortium (BT3) achieved the highest TPH degradation (78.3%) and demonstrated significant reduction in long-chain hydrocarbon fractions (C₁₄-C₂₈). Toxicity measurements showed that all three bioremediation treatments, especially BT3, significantly increased earthworm survival, body weight change and plant germination rate after the bioremediation. Microbial community analysis based on 16S rRNA sequencing revealed significant shifts in the dominant bacterial genera over time, accompanied by a noticeable reduction in alpha diversity. In particular, BT3 showed a significant decrease in Shannon diversity index values from 9.4 at S₁ to 6.9 at S₃ (p < 0.01), whereas BT1 and BT2 remained relatively stable (p > 0.05). Overall, the results demonstrated that all three bacterial treatments significantly enhanced diesel degradation and reduced soil toxicity under outdoor conditions, highlighting their potential for future large-scale applications in sustainable soil remediation. Importantly, this study combines constructed microbial consortia with multi-level toxicity assessments, providing a comprehensive framework to guide future bioremediation strategies. Full article

Mining activities often result in ecosystem degradation and landscape destruction. The restoration of abandoned mine lands is particularly challenging due to the poor physicochemical properties and low fertility of the soil, which necessitate the use of soil amendments. Sewage sludge, which contains abundant nutrients, has potential for use in mine soil restoration. Four separate piles of sewage sludge, each weighing 5 tons, were subjected to aerobic composting and then applied at different rates (0%, 2%, 5%, 10%, and 20%) to reclaim an abandoned mine land site (500 m²). During the composting process, the pH, moisture content, organic matter (OM), and dissolved organic matter (DOM) of the sewage sludge all decreased, while electrical conductivity (EC) and germination index (GI) increased. The sewage sludge compost reached maturity after 83 days. Soil pH and bulk density decreased with increasing application levels, whereas soil fertility, such as OM, alkali-hydrolyzable nitrogen, available phosphorus (AP), and available potassium (AK), significantly improved after application of sewage sludge compost. Vegetation coverage (ryegrass and alfalfa) reached 100% after 2 months at the 20% application level. Fresh biomass and plant height were significantly higher at all application levels compared to the control (p < 0.05). Results from Pearson’s correlation, redundancy analysis (RDA), and the random forest model indicated that soil fertility, particularly AP, OM, and alkali-hydrolyzable nitrogen, rather than soil physicochemical properties, was the key factor influencing the restoration success of the mine site. The use of sewage sludge compost as a soil amendment for reclaiming abandoned mine lands is feasible and can help reduce the ecological restoration costs of mining. Full article