Search Results (1,345)

Background: Oxidative degradation during deep frying negatively affects the nutritional quality and stability of edible oils. Rapid, non-destructive methods to monitor oxidation, particularly in antioxidant-enriched oils, are therefore of growing interest. Materials and Methods: This study investigates the potential of near-infrared (NIR) spectroscopy combined with chemometric modeling—specifically the Stepwise Decorrelation of Variables (SELECT) algorithm and Ordinary Least Squares (OLS) regression—to quantitatively assess oxidation dynamics in edible oils enriched with hydroxytyrosol extract from olive fruit during deep frying. Extra virgin olive oil, virgin olive oil, refined olive oil, refined sunflower oil, and high-oleic sunflower oil were evaluated under controlled thermal degradation conditions. Results: Variable selection identified key NIR spectral regions related to acidity, conjugated dienes (K₂₃₂), secondary oxidation indices (K₂₇₀ and ΔK), peroxide value (PV), anisidine value (AnV), and the total oxidation (TOTOX) index. From 700 measured wavelengths, a limited number were sufficient for robust prediction (16–30 wavelengths depending on the parameter), with critical sensitivity observed around 1792 nm and 1392 nm. The optimized NIR–SELECT–OLS models showed strong predictive performance across oil types (R² > 0.90; explained variance > 85%). Conclusions: The results demonstrate that hydroxytyrosol enrichment enhances the oxidative and nutritional stability of edible oils during deep frying. Moreover, the integration of NIR spectroscopy with chemometric modeling provides an effective, non-destructive tool for real-time monitoring of oil oxidation, supporting sustainable quality control, process optimization, and antioxidant fortification in functional edible oils. Full article

The agronomic performance and quality of different strawberry (Fragaria x ananassa) varieties produced in Santiago del Estero, Argentina, were studied. The following varieties were evaluated in 2023 and 2024 under semi-forced cultivation and bioinputs: Rociera (RO), Frontera (FR), Sahara (SH), Savana (SV), and Sabrina (SB). The crop performance was evaluated through yield production, and fruit quality was determined through titratable acidity (TA), soluble solids (SSs), firmness, and instrumental color with L* (luminosity), a* (red-green color), and b* (blue-yellow color) parameters. The results obtained indicate that the evaluated varieties had good productive performance, especially FR and RO, through viable production yields. Full article

This study investigated the effects of regulated deficit irrigation on quality and postharvest characteristics of ‘Soreli’ kiwifruit (Actinidia chinensis Planch.). Plants were irrigated at 100% (control), 80%, and 60% of the standard water supply. Fruit quality was monitored by assessing weight loss (WL), firmness, soluble solids content (SSC), and color stability. Bioactive compounds, such as polyphenols (POL), flavonoids (FLAV), ascorbic acid (AA), β-carotene (Car), and chlorophyll (Chl) content and antioxidant enzyme activities, including ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT), and the 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay were also evaluated. Results indicated that reduced irrigation at 60% of water supply enhanced antioxidant enzyme levels, without negatively affecting fruit quality parameters: greater resistance to firmness loss, higher soluble solids accumulation, and better color stability. In the early stages of cold storage, fruits under the 60% irrigation treatment showed higher POL, FLAV, and ABTS values, with polyphenols exceeding 200 mg GAE 100 g⁻¹ FW and FLAV content ranging from 4.69 to 5.53 mg CE 100 g⁻¹ FW. The 80% irrigation treatment showed a moderate biochemical response without altering quality. Controlled water deficit can enhance antioxidant activity and bioactive compounds, improving fruit quality and the environmental and commercial value of ‘Soreli’ kiwifruit. Full article

Soil mulching materials play an important role in regulating the greenhouse crop microclimate, as they influence light distribution, plant physiological activity, and crop yield. The aim of this study was to evaluate the effects of two plastic mulches (black polypropylene and white polyethylene mulch) on the microclimate, photosynthetic activity, crop development, yield, and fruit quality of sweet pepper (Capsicum annuum L.) grown under greenhouse conditions. The trial was developed during a spring–summer growing cycle in a single multispan greenhouse divided into two compartments (sectors) separated by a vertical polyethylene sheet. In the eastern sector of the greenhouse (control treatment), a black polypropylene agrotextile mulch with a thickness of 2500 μm was installed, while in the western sector, a white polyethylene plastic mulch (black on the inner side) with a thickness of 30 μm was used. The use of white polyethylene mulch resulted in slightly higher mean and maximum PAR inside the greenhouse by up to 3.7% compared with black polypropylene mulch, leading to slightly higher leaf-level PAR and net photosynthetic rate. Although no significant differences were observed in plant morphology or fruit quality parameters, marketable yield increased by 66% and total yield by 40% under white polyethylene mulch. Slight increases in internal air temperature were recorded without exceeding critical thresholds, while relative humidity remained largely unaffected. The use of reflective mulches may represent a promising low-cost and sustainable strategy to improve pepper yield and radiation-use efficiency in passively ventilated greenhouse systems under Mediterranean climatic conditions. Full article

In Colombia, two main palm varieties, Elaeis guineensis and Elaeis oleifera, are cultivated for the production of crude palm oil (CPO). During the CPO extraction process, several residues are generated, including empty fruit bunches (EFB), nut fiber, palm kernel cake, and Palm Oil Mill Effluent (POME), among others. These residues are commonly used for biochar and compost production to improve soil quality, for biogas generation, and for energy production through biomass combustion. Because the rachis is rich in lignocellulosic material and exhibits physicochemical properties suitable for thermochemical processes, it is proposed as a feedstock for hydrogen synthesis through gasification. In this study, a techno-economic analysis and an FP²O resilience assessment were conducted for a hydrogen production process based on the utilization of palm rachis generated in María la Baja, northern Colombia. The economic evaluation results indicate that the capital investment required for plant installation is USD 10,111,255.23. The economic indicators show favorable performance with a Return on Investment (ROI) of 58.83%, a Net Present Value (NPV) of USD 25.01 million, a B/C ratio of 3.29, and a Discounted Payback Period (DPBP) of 4.54 years. Regarding techno-economic resilience, critical values for processing capacity, selling price, and feedstock cost were identified through parameter variation. The findings suggest that the process has opportunities for improvement, since small changes in these variables could significantly reduce its resilience. Finally, an On-Stream efficiency of 39.65% at the break-even point was obtained, indicating that the process can operate at less than 50% of its maximum capacity while still generating significant profits. Full article

Table grapes are among the main fruit crops cultivated in Brazil, supported by cultivar diversity, technological advances, and adaptation to diverse edaphoclimatic conditions. Rootstock selection is critical in viticulture, influencing phenology, yield, and fruit quality. This study evaluated yield- and fruit-related production parameters, cluster characteristics, and biochemical composition of ‘BRS Núbia’ table grape grafted onto different rootstocks. The experiment was conducted at the Experimental Farm of the Faculty of Agricultural Sciences (UNESP), São Manuel, São Paulo, Brazil, using a randomized block design in a split-plot scheme (three rootstocks × three seasons) with seven replicates. Rootstocks included ‘IAC 572 Jales’, ‘IAC 766 Campinas’, and ‘Paulsen 1103’, while subplots corresponded to the first three production seasons after grafting. Evaluated variables comprised bud fruitfulness, yield, productivity, physical attributes of clusters, berries, and rachises, and in 2022, berry biochemical traits, including total phenolics, flavonoids, anthocyanins, and antioxidant activity. Rootstocks did not significantly affect bud fruitfulness or yield-related parameters. In contrast, production season markedly influenced vine performance, with the third (2023) season showing higher cluster and berry mass and size. Regarding fruit composition, vines grafted onto ‘Paulsen 1103’ and ‘IAC 766 Campinas’ showed greater accumulation of total phenolics and anthocyanins than those grafted onto ‘IAC 572 Jales’, overall. Full article

The citrus processing industry generates large amounts of organic residues whose sustainable management is a major environmental challenge. The aim of this study was to evaluate the effects of incorporating citrus-derived waste (CW) into coconut-coir-based substrates on tomato (Solanum lycopersicum L., cv. Proxy) under different irrigation regimes (I) in a factorial design (CW × I) with three replications. Each replicate consisted of six plants (pots), and the replicate was considered the experimental unit. Plants were grown in substrates amended with 0%, 6.25%, 12.5%, 25.0%, and 37.5% (v/v) citrus waste and subjected to three water regimes (100%, 75%, and 50% of the standard water supply). Plant growth, biomass allocation, yield components, and fruit quality traits were assessed. Results indicate that CW can be incorporated into coconut-coir substrates without detectable penalties in total production at low-to-moderate rates (6.25–12.5%) across all irrigation regimes. Yield reductions of 18% (from 3398 to 2789 g plant⁻¹) attributable to CW were observed mostly at the highest inclusion rates under moderate deficit irrigation (75% water supply), whereas under severe deficit (50% water supply), production declined across all CW rates, including 0%, indicating that water deficit has a dominant limiting effect. Fruit quality parameters were generally maintained or improved in amended substrates, particularly under reduced irrigation with deficit irrigation, generally increasing total soluble solids at 100%, 75%, and 50% WC (+13%, +19%, and +9%, respectively). Overall, these findings support the use of citrus waste at low-to-moderate proportions as a sustainable amendment for soilless tomato cultivation without marked negative effects on yield and fruit quality, enabling its use as a locally sourced substrate component within circular-economy strategies. Full article

This is the first investigation that attempts to synthesize chitosan-loaded vanillin nanoformulation (vanillin-Nf) as a novel edible coating agent to prolong the storage life of Indian gooseberry (amla). Different concentrations of vanillin were encapsulated into chitosan via ionic gelation approach using sodium tripolyphosphate as a cross-linker. Vanillin-Nf 1:1 (w/v) exhibited maximum loading capacity (2.502 ± 0.008%) and encapsulation efficiency (54.483 ± 1.165%). The physico-chemical characterization of vanillin-Nf through SEM, DLS, FT-IR, and XRD techniques confirmed effective incorporation of vanillin into the chitosan biomatrix and formation of spherical nanocapsules, with a mean particle size of 232.83 nm, zeta potential +69.66 mV, and polydispersity index 0.296. The in vitro release profile of vanillin exhibited a biphasic and regulated release pattern. The application of vanillin-Nf as an edible coating solution on amla (Phyllanthus emblica L.) fruits was highly effective in reducing decay incidence up to 42.84% and extended their shelf-life to 15 days at 25 ± 2 °C. The vanillin-Nf coating significantly reduced weight loss in amla fruits (24.39 ± 1.02%) in comparison to control. In addition, vanillin-Nf coating also helped in preserving the key quality parameters, including pH, chlorophyll content, total soluble solids, total phenols, and antioxidant capacity of Indian gooseberries to a substantial extent at the end of storage. Collectively, our findings indicate that vanillin-Nf coating is an effective post-harvest approach for controlling decay, prolonging shelf-life, and maintaining the nutritional attributes of Indian gooseberries, highlighting its potential for commercial application in the food and agriculture industry. Full article

The use of unconventional water sources, such as those from marine desalination plants, is challenging for agriculture due to boron concentrations exceeding 0.5 mg L⁻¹, which can impact crop yield and quality. To ensure sustainability, it is crucial to understand crop responses to high boron levels and to develop strategies to mitigate its toxic effects. This study evaluated the impact of irrigation with a nutrient solution containing 15 mg L⁻¹ of boron on tomato plants (Solanum lycopersicum L.). To modulate the physiological effects of boron toxicity, two biostimulant products based on an extract from the brown alga Laminaria digitata and other active ingredients were applied foliarly. Agronomic, nutritional, and metabolic parameters were analyzed, including total yield, number of fruits per plant, and fruit quality. Additionally, mineral analysis and metabolomic profiling of leaves and fruits were performed, focusing on amino acids, organic acids, sugars, and other metabolites. A control treatment was irrigated with a nutrient solution containing 0.25 mg L⁻¹ of boron. The results showed that a boron concentration of 15 mg L⁻¹ significantly reduced total yield by 45% and significantly decreased fruit size and firmness. Mineral and metabolomic analyses showed significant reductions in Mg and Ca concentrations, significant increases in P and Zn levels, excessive boron accumulation in leaves and fruits, and significant changes in metabolites associated with nitrogen metabolism and the Krebs cycle. Biostimulant application did not significantly improve agronomic performance, likely due to high boron accumulation in the leaves, although significant changes were detected in leaf nutritional status and metabolic profiles. Full article

Understanding the complex interplay between environmental factors and fruit quality development requires sophisticated analytical approaches linking cellular architecture to environmental conditions. This study introduces a novel application of dual-resolution X-ray computed tomography (CT) for the non-destructive characterization of apple internal tissue architecture in relation to fruit growth, thereby advancing beyond traditional methods that are primarily focused on postharvest analysis. By extracting detailed three-dimensional structural parameters, we reveal tissue porosity and heterogeneity influenced by crop load, maturity timing and canopy position, offering insights into internal quality attributes. Employing correlation analysis, Principal Component Analysis, Canonical Correlation Analysis, and Structural Equation Modeling, we identify temperature as the primary environmental driver, particularly during early developmental stages (45 Days After Full Bloom, DAFB), and uncover nonlinear, hierarchical effects of preharvest environmental factors such as vapor pressure deficit, relative humidity, and light on quality traits. Machine learning models (Multiple Linear Regression, Random Forest, XGBoost) achieve high predictive accuracy (R² > 0.99 for Multiple Linear Regression), with temperature as the key predictor. These baseline results represent findings from a single growing season and require validation across multiple seasons and cultivars before operational application. Temporal analysis highlights the importance of early-stage environmental conditions. Integrating structural and environmental data through innovative visualization tools, such as anatomy-based radar charts, facilitates comprehensive interpretation of complex interactions. This multidisciplinary framework enhances predictive precision and provides a baseline methodology to support precision orchard management under typical agricultural variability. Full article

Banana (Musa spp.) fruit morphology is a key determinant of yield and quality, yet modeling its 3D structural dynamics across genotypes remains difficult. To address this challenge, we developed a generic, biomass-driven 3D structural model for banana fruit fingers that quantitatively links growth and morphology. Field experiments were conducted over two growing seasons in Hainan, China, using three representative genotypes. Morphological traits, including outer and inner arc length, circumference, and pedicel length, along with dry (W_d) and fresh weight (W_f), were measured every 10 days after flowering until 110 days. Quantitative relationships between morphological traits and W_f, as well as between W_d and W_f, were fitted using linear or Gompertz functions with genotype-specific parameters. Based on these functions, a parameterized 3D reconstruction method was implemented in Python, combining biomass-driven growth equations, curvature geometry, and cross-sectional interpolation to simulate the fruit’s bending, tapering, and volumetric development. The resulting dynamic 3D models accurately reproduced genotype-specific differences in curvature, length, and shape with average fitting R² > 0.95. The proposed biomass-driven 3D structural model provides a methodological framework for integrating banana fruit morphology into functional–structural plant models. Full article

This study is Part II of a five-year (2018–2022) field trial in western Portugal evaluating the effects of three microbial biofertilizers—Mycoshell^® (Glomus spp. + humic/fulvic acids), Kiplant iNmass^® (Azospirillum brasilense, Bacillus megaterium, Saccharomyces cerevisiae), and Kiplant All-Grip^® (Bacillus megaterium, Pseudomonas spp.)—applied at different dosages alongside two mineral fertilizer regimes, T100 (full dose) and T70 (70% of T100, alone or combined with biofertilizers), on the physiological performance of ‘Gala Redlum’ apple trees. Part I had shown that Myc4 (Mycoshell^®, 4 tablets/tree), iNM6, and iNM12 (Kiplant iNmass^®, 6 and L ha⁻¹, respectively) consistently enhanced fruit growth, yield, and selected quality traits. While Part I showed clear agronomic gains, Part II demonstrates that these improvements occurred without significant alterations in seasonal photosynthetic performance, canopy reflectance, or chlorophyll fluorescence parameters over five years, highlighting the contrast between observed yield improvements and physiological stability. Seasonal monitoring of physiological traits—including specific leaf area (SLA), chlorophyll content index (CCI), gas exchange (A_n, g_s, E, C_i), spectral indices (NDVI, OSAVI, SIPI, GM2), and chlorophyll fluorescence (OJIP). It is clear that physiological values remained largely stable across biofertilizer treatments and years. Importantly, this stability was maintained even under a 30% reduction in mineral fertilizer (T70), indicating that specific microbial biofertilizers can sustain physiological resilience under reduced nutrient inputs, thereby providing a physiological basis for the yield-enhancing effects observed and supporting their integration into fertilizer reduction strategies in Mediterranean orchards. Full article

Non-destructive monitoring of fruit ripening is essential for optimising harvest time, yet its application to tropical viticulture remains largely unexplored. This study evaluated in situ chlorophyll a fluorescence as a non-invasive physiological marker to track berry development and metabolic maturation in two table grape cultivars (Vitis labrusca L. var. Niagara Rosada and var. Romana) under tropical field conditions, characterised by the latitude position, absence of chilling-induced dormancy, and variable rainfall during ripening. Berries’ fluorescence parameters (Fo, Fm, Fv and Fv/Fm) were monitored weekly from the pea-size stage to commercial harvest (67–123 days after pruning) using a portable modulated fluorometer, along with chlorophyll and quality trait measurements. A decline in fluorescence parameters during maturation coincided with chlorophyll degradation and the accumulation of glucose and fructose. The maximum quantum yield of PSII (Fv/Fm) remained stable (≈0.75) throughout development, indicating sustained photochemical efficiency despite chloroplast disassembly. Significant correlations (r > 0.80) were established between fluorescence parameters and key maturity indices, with distinct cultivar-specific patterns evident between the NR and RM cultivars. Therefore, chlorophyll a fluorescence provided a reliable, portable, non-destructive tool for monitoring ripening dynamics and estimating quality parameters in table grapes, offering practical advantages for tropical viticulture where environmental variability demands flexible monitoring. Full article

Traditional Serbian pepper cultivars ‘Kurtovska ajvaruša’, ‘Grkinja babura’, and ‘Duga bela ljuta’ were stored under different conditions (10 °C, 4 °C, and 4 °C with pre-storage hot water dipping-HWD) for 21 days plus a 3-day shelf life. The main quality parameters measured included mineral content, total soluble solids (TSS), titratable acidity (TA),sugar content (glucose, fructose), organic (ascorbic and citric) acid content, and total phenolic content (TPC). Principal component analysis (PCA) revealed cultivar-specific responses to storage treatments. Cultivar specificity is a crucial determinant in defining optimal conditions for the preservation of phytochemical composition. The cultivar ‘Kurtovska ajvaruša’ showed the highest retention of phenolic compounds when stored at 4 °C following hot water treatment (HWD), whereas ‘Grkinja babura’ should be stored at 4 °C (without hot water treatment, as it provides no additional benefits) for up to 21 days, as this ensures balanced preservation of sugar and organic acid contents while maintaining high sensory quality of the fruit. ‘Duga bela ljuta’ exhibited superior ascorbic acid preservation at 10 °C, reaching 104.4 mg/100 g. Optimizing postharvest storage conditions is essential for maintaining the nutritional quality of traditional pepper cultivars intended for both fresh consumption and processing. Full article

Actinidia deliciosa is a globally important economic fruit crop, and its fruit quality and yield are profoundly influenced by light and environmental conditions. Sucrose phosphate synthase (SPS), a key rate-limiting enzyme in the sucrose biosynthesis pathway, plays a central role in regulating carbon metabolism and sucrose accumulation in plants. However, comprehensive studies of the SPS gene family in A. deliciosa are still lacking, particularly regarding its expression in response to different light qualities. In this study, genome-wide identification of the SPS gene family in A. deliciosa was conducted using bioinformatics approaches. A total of 31 SPS genes were identified and named AdSPS1 to AdSPS31 on the basis of their chromosomal positions. The encoded proteins were predicted to be acidic, hydrophilic, and primarily localized in the chloroplast. All the AdSPS proteins contained the conserved domains Sucrose_synth, Glyco_trans_1, and S6PP, indicating potential roles in sucrose metabolism. Phylogenetic analysis classified the 31 AdSPS members into three subfamilies, A, B, and C, comprising 20, 5, and 6 members, respectively. Collinearity analysis revealed extensive syntenic relationships among AdSPS genes across different chromosomes, suggesting that gene duplication events contributed to the expansion of this gene family. Promoter cis-acting element analysis revealed that light-responsive elements were the most abundant among all the detected elements in the upstream regions of the AdSPS genes, implying potential regulation by light signals. Different light qualities significantly affected the contents of sucrose, glucose, and fructose, as well as SPS activity in kiwifruit leaves, with the highest activity observed under the R3B1 (red–blue light 3:1) treatment. Spearman’s correlation analysis indicated that AdSPS3 was significantly negatively correlated with sucrose, fructose, glucose, and SPS activity, suggesting a potential role in negatively regulating sugar accumulation in kiwifruit leaves, whereas AdSPS12 showed positive correlations with these parameters, implying a role in promoting sucrose synthesis. To further explore the light response of the AdSPS genes, eight representative members were selected for qRT‒PCR analysis under red light, blue light, and combined red‒blue light treatments. These results demonstrated that light quality significantly influenced SPS gene expression. Specifically, AdSPS6 and AdSPS24 were highly responsive to R1B1 (1:1 red‒blue light), AdSPS9 was significantly upregulated under R6B1 (6:1 red‒blue light), AdSPS21 was strongly induced by blue light, and AdSPS12 expression was suppressed. This study systematically identified and analyzed the SPS gene family in A. deliciosa, revealing its structural characteristics and light-responsive expression patterns. These findings suggest that AdSPS genes may play important roles in light-regulated carbon metabolism. These results provide a theoretical foundation and valuable genetic resources for further elucidating the molecular mechanisms of sucrose metabolism and light signal transduction in kiwifruit. Full article