1. Introduction
In regions facing water scarcity, efficient water management is essential to ensure sustainable agricultural production. One widely adopted strategy to improve irrigation efficiency and conserve soil moisture is the use of soil covers, which act as vapour diffusion barriers at the soil–atmosphere interface [
1,
2]. By reducing evaporation losses and promoting transpiration, mulching practices can increase soil water availability, plant biomass, and crop yield [
3,
4,
5,
6].
Mulching consists of applying an organic, synthetic, or inorganic layer over the soil surface to modify heat and water exchange processes. This practice improves soil moisture retention, moderates soil temperature, suppresses weed growth, and enhances microbial activity [
7]. The effects of mulches on soil thermal and hydrological regimes have been extensively documented [
8,
9,
10,
11], with soil temperature responses strongly influenced by the optical and thermal properties of the covering material [
10].
In greenhouse production systems, where energy exchanges among soil, plants, air, and structural components are particularly complex [
12], soil covers play a key role in shaping the microclimate and, consequently, crop performance. The effectiveness of mulching materials depends not only on their physical composition but also on their colour, which determines their optical behaviour and capacity to reflect solar radiation [
13]. In this context, the interaction between mulch properties and soil–plant–atmosphere energy fluxes influence critical processes such as radiation balance, conduction, convection, evaporation, and condensation [
14].
Beyond their microclimatic effects, mulches provide several agronomic benefits, including weed suppression, improved soil thermal regulation, reduced evaporative losses, and earlier crop development [
15,
16]. These advantages often translate into enhanced yield and product quality [
17,
18]. However, the use of plastic mulches also presents limitations, such as increased production costs [
19] and environmental concerns related to plastic waste accumulation [
20]. In warm climates, excessive soil heating under plastic covers may further impair crop performance by inducing thermal stress in the root zone [
21,
22,
23].
In addition to their physical effects, mulches influence plant physiological processes, particularly photosynthesis, which is highly sensitive to changes in the growing environment [
24]. Variations in soil temperature and light reflection can affect gas exchange, leaf development, and overall plant performance [
25]. Since leaves are the primary photosynthetic organs directly linked to yield formation, optimising their functional activity during reproductive stages is crucial [
26,
27]. Several studies have shown that plastic mulching can enhance photosynthetic capacity by improving root-zone conditions and plant water status [
28,
29].
White or reflective mulches can increase the proportion of short-wave radiation reflected toward the canopy, thereby enhancing the availability of photosynthetically active radiation [
30]. However, their effects on crop performance are not always consistent. While some studies report increased yields and reduced incidence of insect-transmitted diseases [
31,
32], others indicate limited benefits or potential drawbacks, such as reduced soil heat accumulation [
33]. Consequently, the suitability of reflective mulches depends on crop type, climate, and production system.
From an environmental perspective, the widespread use of plastic mulches has raised concerns due to the large volumes of agricultural plastic waste generated annually. In Spain alone, up to 35,000 tonnes of plastic residues are produced each year, particularly in intensive horticultural regions such as Andalucía, Castilla-La Mancha, and Murcia [
34].
Consequently, the suitability of reflective mulches depends on crop type, climate, and production system. Pepper (Capsicum annuum L.) is one of the most economically important greenhouse vegetable crops in Mediterranean regions, particularly in southeastern Spain, where it represents a major share of protected horticultural production and farm income.
Against this background, the objective of the present study is to evaluate the effects of different highly reflective soil mulches on microclimate, plant growth, yield, and photosynthetic activity of sweet pepper (Capsicum annuum L.) cultivated under multispan greenhouse conditions during the spring–summer growing season. Two mulching materials were compared: a white polyethylene plastic film and a black polypropylene plastic film.
Despite the extensive use of plastic mulches in greenhouse horticulture, there is still limited information on the effects of reflective soil coverings in multispan greenhouses under Mediterranean conditions, particularly regarding their combined influence on microclimate, leaf-level photosynthetic activity, and crop productivity. Most previous studies have focused on open-field systems or have analysed microclimate and yield responses separately. Therefore, the novelty of the present study lies in the integrated assessment of greenhouse microclimate, photosynthetic activity, and yield response of pepper grown under contrasting mulch optical properties within a multispan greenhouse.
2. Materials and Methods
2.1. Experimental Site
The present study was conducted during the spring–summer 2024 season at the Centre for Innovation and Technology Transfer ‘Fundación UAL-ANECOOP’ (latitude: 36°51′53.2″ N; longitude: 2°16′58.8″ W; altitude: 87 m). A multispan greenhouse (800 m
2, orientation: 118°N) was divided into two similar sectors, East and West (
Table 1), using a vertical plastic sheet as a partition. The greenhouse is equipped with two roof vents, one facing north and the other south, as well as two side vents with a maximum opening of 3 m (
Figure 1).
Ventilation was controlled by Synopta Software 5.4.2.3931422 (Ridder Growing Solutions B.V., Maasdijk, The Netherlands), a centralised climate control and data logging system with a weather station. The temperature setpoint for control of vent opening was 20 °C.
In the eastern sector of the greenhouse (control treatment), a black polypropylene agrotextile mulch with a thickness of 2500 μm—according to manufacturer specifications—was installed, while in the western sector, a white polyethylene plastic mulch (black on the inner side) with a thickness of 30 μm (model E1115, Politiv, Kibbutz Einat, Israel) was used (
Figure 2).
2.2. Crop Systems
To evaluate the effect of plastic mulch on pepper (Capsicum annuum L.) cultivation, a spring–summer growing cycle was conducted using the commercial cultivar Bemol RZ (Rijk Zwaan Ibérica, S.A., Almería, Spain). Transplanting was carried out on 5 March 2024 onto a coconut fibre substrate at a planting density of 1 plant m−2, with crop rows oriented perpendicular to the greenhouse ridge. Fertigation was uniformly applied in both experimental sectors through a drip irrigation system managed by a Supra irrigation controller (Hermisan, Alicante, Spain). Irrigation scheduling, water volume, and nutrient solution composition were identical in both sectors throughout the experiment. Standard crop management practices, including cleaning, trellising, pruning, and harvesting, were performed simultaneously in both sectors.
2.3. Microclimate Measurement Equipment
In the centre of each sector, at 2 m height, there was an aspirated radiation shield box EKTRON II-C (Ridder Growing Solutions B.V.) within which there were a Pt1000 class B temperature sensor (Vaisala Oyj, Helsinki, Finland) with a measurement range of −10 to 60 °C and an accuracy of ±0.6 °C, a capacitive humidity sensor HUMICAP 180R (Vaisala Oyj, Helsinki, Finland) with a measurement range of 0–100% and an accuracy of ±3%, and a CO2 Probe EE871 (Elektronik Ges M.b.h. Engerwitzdorf, Austria) with a measurement range of 0–2000 ppm and accuracy of ±2% from the measured value (m.v.). Outside climatic conditions were recorded by a meteorological station at a height of 9 m equipped with a BUTRON II (Ridder Growing Solutions B.V.) measurement box with similar temperature and humidity sensors to the inside measurement box.
2.4. Measurement of Photosynthetic Activity
Alternate routes were established between the eastern and western sectors of the greenhouse, encompassing a total of 16 measurement rows (eight in the northern section and eight in the southern section) (
Figure 1b). Photosynthetic activity was measured eight times during the season (at 59, 71, 83, 86, 104, 108, 120, and 125 days after transplanting (DAT)), resulting in a total of 380 measurements per sector. Three plants were selected per row, with two measurements taken for each plant. A portable photosynthesis system TARGAS 1 (PP Systems, Amesbury, MA, USA) was used with a blade clamping chamber equipped with an IRGA sensor for CO
2 and H
2O concentration. The measurement ranges were 0–10,000 ppm for CO
2 (accuracy ±1%), 0–75 mbar for H
2O (accuracy ±1%) and 0–3000 μmol m
−2 s
−1 for PAR (accuracy ±10 μmol m
−2 s
−1). The photosynthetic activity (P
A), PAR reaching the leaf surface (Q
leaf), leaf temperature (T
L), CO
2 concentration in the leaf environment (C
L), and transpiration rate (T
R) were measured on mature and fully expanded leaves [
35] on different plants and days during the crop season, under natural inside light and ambient CO
2 concentration, between 10:00 and 15:00 h [
36].
2.5. Equipment for Crop Development and Yield Measurements
To evaluate crop development, two plant rows (R1–R2), considered as statistical replicates, were randomly selected in each sector, with eight plants per row (four facing north and four facing south) (
Figure 1). Growth parameters were measured five times during the season (at 37, 51, 65, 79, and 92 days after transplanting (DAT)), resulting in a total of 40 measurements per sector. Measurements were taken using a tape measure and a digital calliper with a measuring range of 0–150 mm and an accuracy of 0.01 mm (Medid Precision S.A., Barcelona, Spain). Morphological parameters were recorded on the same plants throughout the season, following the IPGRI [
37] guidelines. The traits assessed included: plant height (P
H) [cm]; plant width (P
W) [cm]; stem diameter (S
D) [mm]; number of nodes (N
N) and internode length (I
L) [cm].
Five harvests were carried out to assess yield. During each harvest, all marketable and non-marketable fruits from the plants in three rows (R1–R3) per sector were weighed (
Figure 1b). Harvests were carried out weekly, at 98, 105, 113, 120, and 134 DAT. Fruits were weighed with a Mettler Toledo electronic scale (Mettler-Toledo, Barcelona, Spain), with a maximum capacity of 60 kg and a sensitivity of 20 g.
To evaluate fruit quality, three plant rows per treatment were selected (R1–R3). In each row, ten fruits (five from the north-facing side and five from the south-facing side) were sampled at each harvest. The following parameters were measured: fruit weight (WF) [g], measured with an electronic balance PB3002-L DeltaRange® (Mettler Toledo, S.A., Spain; capacity: 600–3100 g; sensitivity: 0.01–0.1 g); fruit length (LF) [cm] and fruit width (WF) [cm], measured with a 150 mm digital calliper (Medid Precisión S.A., Barcelona, Spain); pericarp thickness (PT) [mm], measured 25 mm above the fruit base using the same digital calliper; pedicel length (PL) [cm], measured with a 150 mm digital calliper; soluble solids content (SSC) [°Brix], measured with a PAL-1 digital refractometer (Atago Co., Ltd., Tokio, Japan; range: 0.0–53.0%, resolution: 0.1%, accuracy: ±0.2%, at 10–40 °C) using a few drops of freshly extracted pepper juice; fruit firmness (FF) [kg cm−2], assessed using a digital penetrometer PCE-FM 200 (PCE-Ibérica S.L., Albacete Spain); resolution: 10 g/0.05 N; accuracy: ±0.5%) by performing three measurements evenly distributed along the equatorial zone of each fruit; dry matter content (DMC) [%], determined after oven-drying at 70 °C for 48 h in a convection oven (23–240 I-FD series, Binder GmbH, Tuttlingen, Germany); and fruit colour, measured with a portable chroma meter CR-400 (Konica Minolta, Tokyo, Japan), using an 8 mm measurement aperture and a six-silicon photodiode detector system to capture L* (lightness), a* (green to red), and b* (blue to yellow) parameters, with three measurements taken at different points around each fruit.
2.6. Statistical Analysis
The data analysed correspond to the results obtained during a spring–summer crop cycle in 2024. For the mulch treatment, the experimental unit was the greenhouse sector (eastern vs. western sector). Measurements performed at plant and row level were considered subsamples within each sector.
Growth and photosynthetic parameters were assessed on 8 and 12 plants, respectively, within each experimental sector. At each harvest, ten pepper fruits were sampled to assess yield quality. Mean values at sector level were used for between-treatment comparisons.
Results were analysed using a multifactorial ANOVA procedure [
38] in Statgraphics 19
® Centurion, considering differences significant at
p ≤ 0.05. Mean values were compared using Fisher’s Least Significant Difference (LSD) test. Factors considered in the analysis included greenhouse sector (2 levels), plant row (3 levels), and harvest date (5 levels), with crop season treated as an additional factor (1 level).
Prior to analysis, the normality of the data was assessed using the Kolmogorov–Smirnov test. Homogeneity of variances between the two sectors was assessed using Bartlett’s, Cochran’s, and Hartley’s tests. When significant differences in standard deviations were detected, parametric ANOVA was considered inadequate. In such cases, a non-parametric analysis was performed using Friedman’s test, considering plant row as a blocking factor and harvest date as the repeated measure.
Given that the experiment was conducted in a single greenhouse divided into two sectors, the statistical analyses should be interpreted with caution, and the results are presented as exploratory and context-specific.
4. Conclusions
In this study, black polypropylene agrotextile mulch (2500 μm thick) and white polyethylene plastic mulch (30 μm thick) were compared in a multispan greenhouse in Almería (Spain) during a spring–summer growing cycle. Under these specific experimental conditions, white polyethylene mulch improved the internal light environment, resulting in higher photosynthetically active radiation at canopy and leaf levels and a moderate increase in leaf-level photosynthetic activity.
Although no significant effects were observed on plant morphology or most fruit quality parameters, the use of white polyethylene mulch was associated with a substantial increase in marketable and total yield. This response was mainly related to greater fruit weight and improved radiation distribution within the greenhouse.
White polyethylene mulch slightly increased internal air temperature without exceeding optimal thresholds for pepper cultivation, while relative humidity remained largely unaffected. Overall, these results provide preliminary evidence that reflective white polyethylene mulch may represent a promising and low-cost strategy to improve radiation availability and yield in greenhouse pepper production under Mediterranean conditions. However, further studies with replicated greenhouses and growing seasons are required to confirm the general applicability of these findings.