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Article

Effect of Reflective Plastic Mulches on the Microclimate, Photosynthetic Activity, and Yield of Pepper (Capsicum annuum L.) in a Multispan Greenhouse

by
María Ángeles Moreno-Teruel
,
Francisco Domingo Molina-Aiz
,
Mireille Nathalie Honoré
,
Alejandro López-Martínez
* and
Diego Luis Valera-Martínez
*
Research Centre CIAIMBITAL, University of Almería, Ctra. de Sacramento s/n, 04120 Almería, Spain
*
Authors to whom correspondence should be addressed.
Horticulturae 2026, 12(2), 174; https://doi.org/10.3390/horticulturae12020174 (registering DOI)
Submission received: 31 December 2025 / Revised: 22 January 2026 / Accepted: 29 January 2026 / Published: 31 January 2026

Abstract

Soil mulching materials play an important role in regulating the greenhouse crop microclimate, as they influence light distribution, plant physiological activity, and crop yield. The aim of this study was to evaluate the effects of two plastic mulches (black polypropylene and white polyethylene mulch) on the microclimate, photosynthetic activity, crop development, yield, and fruit quality of sweet pepper (Capsicum annuum L.) grown under greenhouse conditions. The trial was developed during a spring–summer growing cycle in a single multispan greenhouse divided into two compartments (sectors) separated by a vertical polyethylene sheet. In the eastern sector of the greenhouse (control treatment), a black polypropylene agrotextile mulch with a thickness of 2500 μm was installed, while in the western sector, a white polyethylene plastic mulch (black on the inner side) with a thickness of 30 μm was used. The use of white polyethylene mulch resulted in slightly higher mean and maximum PAR inside the greenhouse by up to 3.7% compared with black polypropylene mulch, leading to slightly higher leaf-level PAR and net photosynthetic rate. Although no significant differences were observed in plant morphology or fruit quality parameters, marketable yield increased by 66% and total yield by 40% under white polyethylene mulch. Slight increases in internal air temperature were recorded without exceeding critical thresholds, while relative humidity remained largely unaffected. The use of reflective mulches may represent a promising low-cost and sustainable strategy to improve pepper yield and radiation-use efficiency in passively ventilated greenhouse systems under Mediterranean climatic conditions.

1. Introduction

In regions facing water scarcity, efficient water management is essential to ensure sustainable agricultural production. One widely adopted strategy to improve irrigation efficiency and conserve soil moisture is the use of soil covers, which act as vapour diffusion barriers at the soil–atmosphere interface [1,2]. By reducing evaporation losses and promoting transpiration, mulching practices can increase soil water availability, plant biomass, and crop yield [3,4,5,6].
Mulching consists of applying an organic, synthetic, or inorganic layer over the soil surface to modify heat and water exchange processes. This practice improves soil moisture retention, moderates soil temperature, suppresses weed growth, and enhances microbial activity [7]. The effects of mulches on soil thermal and hydrological regimes have been extensively documented [8,9,10,11], with soil temperature responses strongly influenced by the optical and thermal properties of the covering material [10].
In greenhouse production systems, where energy exchanges among soil, plants, air, and structural components are particularly complex [12], soil covers play a key role in shaping the microclimate and, consequently, crop performance. The effectiveness of mulching materials depends not only on their physical composition but also on their colour, which determines their optical behaviour and capacity to reflect solar radiation [13]. In this context, the interaction between mulch properties and soil–plant–atmosphere energy fluxes influence critical processes such as radiation balance, conduction, convection, evaporation, and condensation [14].
Beyond their microclimatic effects, mulches provide several agronomic benefits, including weed suppression, improved soil thermal regulation, reduced evaporative losses, and earlier crop development [15,16]. These advantages often translate into enhanced yield and product quality [17,18]. However, the use of plastic mulches also presents limitations, such as increased production costs [19] and environmental concerns related to plastic waste accumulation [20]. In warm climates, excessive soil heating under plastic covers may further impair crop performance by inducing thermal stress in the root zone [21,22,23].
In addition to their physical effects, mulches influence plant physiological processes, particularly photosynthesis, which is highly sensitive to changes in the growing environment [24]. Variations in soil temperature and light reflection can affect gas exchange, leaf development, and overall plant performance [25]. Since leaves are the primary photosynthetic organs directly linked to yield formation, optimising their functional activity during reproductive stages is crucial [26,27]. Several studies have shown that plastic mulching can enhance photosynthetic capacity by improving root-zone conditions and plant water status [28,29].
White or reflective mulches can increase the proportion of short-wave radiation reflected toward the canopy, thereby enhancing the availability of photosynthetically active radiation [30]. However, their effects on crop performance are not always consistent. While some studies report increased yields and reduced incidence of insect-transmitted diseases [31,32], others indicate limited benefits or potential drawbacks, such as reduced soil heat accumulation [33]. Consequently, the suitability of reflective mulches depends on crop type, climate, and production system.
From an environmental perspective, the widespread use of plastic mulches has raised concerns due to the large volumes of agricultural plastic waste generated annually. In Spain alone, up to 35,000 tonnes of plastic residues are produced each year, particularly in intensive horticultural regions such as Andalucía, Castilla-La Mancha, and Murcia [34].
Consequently, the suitability of reflective mulches depends on crop type, climate, and production system. Pepper (Capsicum annuum L.) is one of the most economically important greenhouse vegetable crops in Mediterranean regions, particularly in southeastern Spain, where it represents a major share of protected horticultural production and farm income.
Against this background, the objective of the present study is to evaluate the effects of different highly reflective soil mulches on microclimate, plant growth, yield, and photosynthetic activity of sweet pepper (Capsicum annuum L.) cultivated under multispan greenhouse conditions during the spring–summer growing season. Two mulching materials were compared: a white polyethylene plastic film and a black polypropylene plastic film.
Despite the extensive use of plastic mulches in greenhouse horticulture, there is still limited information on the effects of reflective soil coverings in multispan greenhouses under Mediterranean conditions, particularly regarding their combined influence on microclimate, leaf-level photosynthetic activity, and crop productivity. Most previous studies have focused on open-field systems or have analysed microclimate and yield responses separately. Therefore, the novelty of the present study lies in the integrated assessment of greenhouse microclimate, photosynthetic activity, and yield response of pepper grown under contrasting mulch optical properties within a multispan greenhouse.

2. Materials and Methods

2.1. Experimental Site

The present study was conducted during the spring–summer 2024 season at the Centre for Innovation and Technology Transfer ‘Fundación UAL-ANECOOP’ (latitude: 36°51′53.2″ N; longitude: 2°16′58.8″ W; altitude: 87 m). A multispan greenhouse (800 m2, orientation: 118°N) was divided into two similar sectors, East and West (Table 1), using a vertical plastic sheet as a partition. The greenhouse is equipped with two roof vents, one facing north and the other south, as well as two side vents with a maximum opening of 3 m (Figure 1).
Ventilation was controlled by Synopta Software 5.4.2.3931422 (Ridder Growing Solutions B.V., Maasdijk, The Netherlands), a centralised climate control and data logging system with a weather station. The temperature setpoint for control of vent opening was 20 °C.
In the eastern sector of the greenhouse (control treatment), a black polypropylene agrotextile mulch with a thickness of 2500 μm—according to manufacturer specifications—was installed, while in the western sector, a white polyethylene plastic mulch (black on the inner side) with a thickness of 30 μm (model E1115, Politiv, Kibbutz Einat, Israel) was used (Figure 2).

2.2. Crop Systems

To evaluate the effect of plastic mulch on pepper (Capsicum annuum L.) cultivation, a spring–summer growing cycle was conducted using the commercial cultivar Bemol RZ (Rijk Zwaan Ibérica, S.A., Almería, Spain). Transplanting was carried out on 5 March 2024 onto a coconut fibre substrate at a planting density of 1 plant m−2, with crop rows oriented perpendicular to the greenhouse ridge. Fertigation was uniformly applied in both experimental sectors through a drip irrigation system managed by a Supra irrigation controller (Hermisan, Alicante, Spain). Irrigation scheduling, water volume, and nutrient solution composition were identical in both sectors throughout the experiment. Standard crop management practices, including cleaning, trellising, pruning, and harvesting, were performed simultaneously in both sectors.

2.3. Microclimate Measurement Equipment

In the centre of each sector, at 2 m height, there was an aspirated radiation shield box EKTRON II-C (Ridder Growing Solutions B.V.) within which there were a Pt1000 class B temperature sensor (Vaisala Oyj, Helsinki, Finland) with a measurement range of −10 to 60 °C and an accuracy of ±0.6 °C, a capacitive humidity sensor HUMICAP 180R (Vaisala Oyj, Helsinki, Finland) with a measurement range of 0–100% and an accuracy of ±3%, and a CO2 Probe EE871 (Elektronik Ges M.b.h. Engerwitzdorf, Austria) with a measurement range of 0–2000 ppm and accuracy of ±2% from the measured value (m.v.). Outside climatic conditions were recorded by a meteorological station at a height of 9 m equipped with a BUTRON II (Ridder Growing Solutions B.V.) measurement box with similar temperature and humidity sensors to the inside measurement box.

2.4. Measurement of Photosynthetic Activity

Alternate routes were established between the eastern and western sectors of the greenhouse, encompassing a total of 16 measurement rows (eight in the northern section and eight in the southern section) (Figure 1b). Photosynthetic activity was measured eight times during the season (at 59, 71, 83, 86, 104, 108, 120, and 125 days after transplanting (DAT)), resulting in a total of 380 measurements per sector. Three plants were selected per row, with two measurements taken for each plant. A portable photosynthesis system TARGAS 1 (PP Systems, Amesbury, MA, USA) was used with a blade clamping chamber equipped with an IRGA sensor for CO2 and H2O concentration. The measurement ranges were 0–10,000 ppm for CO2 (accuracy ±1%), 0–75 mbar for H2O (accuracy ±1%) and 0–3000 μmol m−2 s−1 for PAR (accuracy ±10 μmol m−2 s−1). The photosynthetic activity (PA), PAR reaching the leaf surface (Qleaf), leaf temperature (TL), CO2 concentration in the leaf environment (CL), and transpiration rate (TR) were measured on mature and fully expanded leaves [35] on different plants and days during the crop season, under natural inside light and ambient CO2 concentration, between 10:00 and 15:00 h [36].

2.5. Equipment for Crop Development and Yield Measurements

To evaluate crop development, two plant rows (R1–R2), considered as statistical replicates, were randomly selected in each sector, with eight plants per row (four facing north and four facing south) (Figure 1). Growth parameters were measured five times during the season (at 37, 51, 65, 79, and 92 days after transplanting (DAT)), resulting in a total of 40 measurements per sector. Measurements were taken using a tape measure and a digital calliper with a measuring range of 0–150 mm and an accuracy of 0.01 mm (Medid Precision S.A., Barcelona, Spain). Morphological parameters were recorded on the same plants throughout the season, following the IPGRI [37] guidelines. The traits assessed included: plant height (PH) [cm]; plant width (PW) [cm]; stem diameter (SD) [mm]; number of nodes (NN) and internode length (IL) [cm].
Five harvests were carried out to assess yield. During each harvest, all marketable and non-marketable fruits from the plants in three rows (R1–R3) per sector were weighed (Figure 1b). Harvests were carried out weekly, at 98, 105, 113, 120, and 134 DAT. Fruits were weighed with a Mettler Toledo electronic scale (Mettler-Toledo, Barcelona, Spain), with a maximum capacity of 60 kg and a sensitivity of 20 g.
To evaluate fruit quality, three plant rows per treatment were selected (R1–R3). In each row, ten fruits (five from the north-facing side and five from the south-facing side) were sampled at each harvest. The following parameters were measured: fruit weight (WF) [g], measured with an electronic balance PB3002-L DeltaRange® (Mettler Toledo, S.A., Spain; capacity: 600–3100 g; sensitivity: 0.01–0.1 g); fruit length (LF) [cm] and fruit width (WF) [cm], measured with a 150 mm digital calliper (Medid Precisión S.A., Barcelona, Spain); pericarp thickness (PT) [mm], measured 25 mm above the fruit base using the same digital calliper; pedicel length (PL) [cm], measured with a 150 mm digital calliper; soluble solids content (SSC) [°Brix], measured with a PAL-1 digital refractometer (Atago Co., Ltd., Tokio, Japan; range: 0.0–53.0%, resolution: 0.1%, accuracy: ±0.2%, at 10–40 °C) using a few drops of freshly extracted pepper juice; fruit firmness (FF) [kg cm−2], assessed using a digital penetrometer PCE-FM 200 (PCE-Ibérica S.L., Albacete Spain); resolution: 10 g/0.05 N; accuracy: ±0.5%) by performing three measurements evenly distributed along the equatorial zone of each fruit; dry matter content (DMC) [%], determined after oven-drying at 70 °C for 48 h in a convection oven (23–240 I-FD series, Binder GmbH, Tuttlingen, Germany); and fruit colour, measured with a portable chroma meter CR-400 (Konica Minolta, Tokyo, Japan), using an 8 mm measurement aperture and a six-silicon photodiode detector system to capture L* (lightness), a* (green to red), and b* (blue to yellow) parameters, with three measurements taken at different points around each fruit.

2.6. Statistical Analysis

The data analysed correspond to the results obtained during a spring–summer crop cycle in 2024. For the mulch treatment, the experimental unit was the greenhouse sector (eastern vs. western sector). Measurements performed at plant and row level were considered subsamples within each sector.
Growth and photosynthetic parameters were assessed on 8 and 12 plants, respectively, within each experimental sector. At each harvest, ten pepper fruits were sampled to assess yield quality. Mean values at sector level were used for between-treatment comparisons.
Results were analysed using a multifactorial ANOVA procedure [38] in Statgraphics 19® Centurion, considering differences significant at p ≤ 0.05. Mean values were compared using Fisher’s Least Significant Difference (LSD) test. Factors considered in the analysis included greenhouse sector (2 levels), plant row (3 levels), and harvest date (5 levels), with crop season treated as an additional factor (1 level).
Prior to analysis, the normality of the data was assessed using the Kolmogorov–Smirnov test. Homogeneity of variances between the two sectors was assessed using Bartlett’s, Cochran’s, and Hartley’s tests. When significant differences in standard deviations were detected, parametric ANOVA was considered inadequate. In such cases, a non-parametric analysis was performed using Friedman’s test, considering plant row as a blocking factor and harvest date as the repeated measure.
Given that the experiment was conducted in a single greenhouse divided into two sectors, the statistical analyses should be interpreted with caution, and the results are presented as exploratory and context-specific.

3. Results

3.1. Climatic Parameter

White mulches installed in the western sector of the greenhouse were primarily intended to reflect a greater proportion of the incoming solar radiation. In both experimental sectors, photosynthetically active radiation (PAR) was measured at a central point, while temperature and relative humidity were recorded at three locations: the centre, south, and north of each sector. The PAR values measured in the centre of the eastern and western sectors of the greenhouse consistently showed higher values in the western sectors with white mulch (Table 2). The use of white plastic mulch was associated with 3.7% higher average PAR values and 2.0% higher maximum values (Table 2).
The western sector, covered with white polyethylene mulch, consistently exhibited higher levels of PAR compared to the eastern sector, which was mulched with black polypropylene. This pattern was more pronounced in the maximum PAR values, particularly on days with higher external radiation. These results suggest that the use of white polyethylene mulch contributes to an improved light environment within the greenhouse, possibly due to its higher reflectance and superior light diffusion properties. In contrast, the black polypropylene mulch appears to absorb a greater proportion of the incoming radiation, resulting in reduced PAR availability within the crop canopy zone.
The observed increase in PAR at 2 m above ground level may be attributed to the fact that a portion of the radiation reflected by the ground is subsequently reflected a second time upon reaching the inner surface of the plastic roof covering. Although external radiation tends to increase throughout the crop growth cycle, the application of whitewash on the greenhouse roof prior to pepper transplanting combined with dust accumulation due to multiple calima (Saharan dust) episodes leads to a progressive reduction in the maximum radiation available inside the greenhouse (Figure 3). Similar effects of mulch reflectivity on light distribution have been reported by Díaz-Pérez [39] and Ilic et al. [40], who found that reflective or light-coloured mulches increase PAR interception and enhance photosynthetic activity and crop performance.
Overall, the differences in radiation transmission between mulch types underline the importance of ground cover selection as a factor influencing the greenhouse microclimate and, consequently, crop photosynthetic efficiency and yield potential.
In general, the mean air temperatures at the centre of the four analysed sectors were very similar. However, an increase in maximum temperature values was observed, possibly due to the rise in radiation resulting from the previously discussed double reflection phenomenon. Similarly to the pattern observed in the mean values, the minimum temperatures recorded at night were highly homogeneous across the 12 measurement points (north, centre, and south of each of the four analysed sectors) (Table 3).
Throughout the measurement period, the mean internal air temperature in both remained close to 20 °C, which corresponds to the ventilation setpoint (Figure 4). Despite the gradual increase in external air temperature during the crop cycle, the internal maximum temperatures remained relatively stable due to the high ventilation capacity of the experimental greenhouses where the trials were conducted. This buffering effect reflects the expected performance of controlled ventilation systems and highlights their importance in maintaining optimal thermal conditions [41,42].
Inside temperatures were consistently higher than those recorded outdoors, particularly at night and during early morning hours. This demonstrates the thermal insulation effect of the greenhouse structure, which helps avoid temperature extremes and protects crops from thermal stress. The western sector (white polyethylene mulch) exhibited slightly higher mean and maximum air temperatures than the eastern sector (black polypropylene mulch). This can be attributed to the higher reflectivity and diffusive capacity of white mulches, which promote greater light scattering and surface warming during daylight [23,40].
In contrast, the eastern sector with black polypropylene mulch showed slightly lower internal air temperatures, likely due to its lower reflectance and higher absorptivity, which concentrates heat near the soil surface rather than distributing it into the air [39,43]. Overall, the choice of ground cover significantly influenced thermal behaviour within the greenhouse. White polyethylene mulch improved the luminous and thermal environment, contributing to more favourable conditions for crop development. These effects underline the role of mulch type not only in light distribution but also in moderating temperature dynamics within greenhouse microclimates [44,45].
In the case of relative humidity, the mean, maximum, and minimum values recorded inside the greenhouse (Table 4) were very similar.
The daily mean relative humidity values (Figure 5a) inside the greenhouse remained relatively stable, consistently around 60% throughout the crop cycle, indicating a high degree of environmental regulation. However, the relative humidity trends also showed a strong dependence on external meteorological conditions.
No substantial differences in mean relative humidity were observed between the western sector (white polyethylene mulch) and the eastern sector (black polypropylene mulch). Nonetheless, more distinct differences appeared in the minimum relative humidity values (Figure 5c) recorded during midday when internal temperatures peaked. The eastern sector consistently exhibited lower minimum relative humidity values, likely due to greater absorption of solar radiation by the black mulch, which tended to increase air temperature and reduce relative humidity (Figure 4). Conversely, the reflective white mulch helped moderate these extremes by improving light distribution and reducing soil heating, thus maintaining slightly higher RH levels during the warmest periods [40,46].
Maximum relative humidity values (Figure 5b), usually observed during early morning hours, often exceeded 90% in both treatments. Although differences between treatments were small, the western sector occasionally showed slightly higher maximum relative humidity values, possibly due to reduced night-time heat losses associated with the more reflective mulch surface [39]. Overall, these results highlight that although mulch type has a moderate influence on relative humidity dynamics, the greenhouse internal humidity is largely driven by external climatic variability.

3.2. Agronomic Parameter

3.2.1. Photosynthetic Activity

The use of white polyethylene mulch in the western sector resulted in a 3.8% increase in photosynthetically active radiation (PAR) at the leaf level compared with the black polypropylene mulch used in the eastern sector (Figure 6b), with average values of 554.9 μmol m−2 s−1 and 534.8 μmol m−2 s−1, respectively (Table 5). This response is consistent with previous reports indicating that light-coloured plastic mulches enhance the reflection and diffusion of incoming radiation toward the crop canopy [23,39].
Similarly, photosynthetic activity was 3.8% higher in plants grown over white polyethylene mulch (Figure 6a), reaching a mean value of 9.8 μmol CO2 m−2 s−1, compared with 9.2 μmol CO2 m−2 s−1 in plants grown over black polypropylene (Table 5). However, the high variability observed in the measurements, reflected in the interquartile ranges and extreme values shown in Figure 6, prevented the detection of statistically significant differences between treatments (Table 5), a common outcome in gas-exchange studies conducted under greenhouse conditions [47].
Leaf temperature was slightly higher in plants grown on black polypropylene mulch (28.9 °C) than in those grown on white polyethylene mulch (28.4 °C), which may be attributed to greater energy absorption by the darker material [23]. In contrast, leaf-level CO2 concentration (421.9 and 420.3 ppm), evapotranspiration (3.2 and 3.4 mmol m−2 s−1), and stomatal conductance did not differ significantly between treatments (Table 5), indicating that under the conditions of this study, mulch type primarily influenced radiation availability rather than inducing substantial changes in plant physiological responses.

3.2.2. Plant Morphology

In general, no statistically significant differences were observed among the evaluated morphological parameters. Although slight numerical differences were detected between treatments, none of them reached statistical significance (Table 6), indicating that the type of mulch did not have a relevant effect on vegetative growth under the conditions evaluated.

3.2.3. Pepper Yield and Fruit Quality

The analysis of the marketable production of the pepper crop showed an increase of 0.63 kg m−2 under the white polyethylene mulch compared with the black polypropylene mulch (Figure 7), which corresponds to an increase of approximately 66% in marketable yield. A similar trend was observed for total yield, with an increase of 0.60 kg m−2 (around 40%) under white polyethylene mulch. This production improvement may be attributed to the effects of mulch optical properties on the crop microenvironment. Plastic mulches with higher reflectance can modify the light available within the plant canopy and alter root-zone temperatures, which in turn can influence plant growth and yield responses [39]. In bell pepper, both white and reflective mulches have been shown to increase fruit yields compared to black plastic, likely due to increased light reflected into the canopy and enhanced plant physiological activity [48]. Similarly, there are reports that white inter-row mulch and reflective mulch treatments produced greater marketable yields than standard black plastic mulch in bell pepper, which supports the yield increases observed here [49]. Additionally, studies on hot pepper have documented the effects of mulch reflectivity on yield potential, although the magnitude and statistical significance vary with environmental conditions [50].
In the statistical analysis of fruit quality parameters, statistically significant differences were observed only for fruit weight. The average fruit weight increased from 173.4 g in the East sector, where black polypropylene mulch was used, to 201.3 g in the West sector with white polyethylene mulch, representing an approximate increase of 16% (Table 7). Similar results were reported by Díaz-Pérez [39], who indicated that the use of plastic mulches can increase fruit weight in pepper due to improvements in the soil microclimate, although such differences do not always reach statistical significance.
Likewise, fruit length showed a slight increase in the sector with white mulch (8.0 cm compared with 7.8 cm) (Table 7), which is consistent with studies reporting that plastic films may promote vegetative growth and fruit size without necessarily producing statistically significant differences among treatments [39,51].
Regarding fruit width and firmness, values were nearly identical between treatments (Table 7), which agrees with reports indicating that these parameters are generally less sensitive to the type of mulch used [52]. Similarly, soluble solids content and dry matter percentage showed only minor variations between sectors, with slightly higher values in the East sector; however, these differences were not statistically significant (Table 7). Previous studies have shown that although plastic mulching can modify the root-zone microclimate and water availability, such changes do not always result in consistent increases in sugar content or dry matter accumulation in the fruit [39,52].
Overall, these results suggest that the use of different plastic mulches under greenhouse conditions may lead to slight variations in fruit quality parameters, but without statistically significant effects. This agrees with previous findings indicating that the main benefits of plastic mulching are more closely related to improvements in yield and microclimate management than to substantial changes in internal fruit quality [39,51].
The colorimetric analysis of pepper fruits showed no statistically significant differences between treatments for any of the evaluated parameters (Table 8). The luminosity coordinate (L*) presented very similar values in fruits harvested under black polypropylene (33.2) and white polyethylene mulch (33.0), indicating comparable surface brightness. Similar results have been reported by Díaz-Pérez [39], who observed that variations in plastic mulch type did not significantly affect lightness values in pepper fruits grown under protected conditions, despite differences in the radiation environment.
The red–green chromatic coordinate (a*) also showed no significant differences between treatments, with mean values of 25.7 and 24.5 for black and white plastic mulches, respectively. Comparable findings were reported by López-Marín et al. [52], who observed that mulch-induced changes in microclimate did not significantly modify pigment accumulation related to red coloration in pepper fruits. Likewise, the yellow–blue coordinate (b*) showed only minor variations between sectors, with slightly higher values in fruits grown under white polyethylene, although these differences were not statistically significant. Similar trends were reported by El-Tantawy et al. [51], who indicated that while mulching can influence fruit development, its effect on colorimetric parameters is often limited.
The chromaticity index (a*/b*), commonly used as an indicator of fruit maturity and carotenoid accumulation, also showed comparable values between treatments. This suggests that, despite the differences in plastic mulch type, fruit ripening and pigment synthesis followed similar patterns in both sectors. These results are consistent with those reported by Díaz-Pérez [39], who concluded that changes in soil covering materials may influence microclimatic conditions but do not necessarily translate into significant differences in the colour development of pepper fruits.

4. Conclusions

In this study, black polypropylene agrotextile mulch (2500 μm thick) and white polyethylene plastic mulch (30 μm thick) were compared in a multispan greenhouse in Almería (Spain) during a spring–summer growing cycle. Under these specific experimental conditions, white polyethylene mulch improved the internal light environment, resulting in higher photosynthetically active radiation at canopy and leaf levels and a moderate increase in leaf-level photosynthetic activity.
Although no significant effects were observed on plant morphology or most fruit quality parameters, the use of white polyethylene mulch was associated with a substantial increase in marketable and total yield. This response was mainly related to greater fruit weight and improved radiation distribution within the greenhouse.
White polyethylene mulch slightly increased internal air temperature without exceeding optimal thresholds for pepper cultivation, while relative humidity remained largely unaffected. Overall, these results provide preliminary evidence that reflective white polyethylene mulch may represent a promising and low-cost strategy to improve radiation availability and yield in greenhouse pepper production under Mediterranean conditions. However, further studies with replicated greenhouses and growing seasons are required to confirm the general applicability of these findings.

Author Contributions

Conceptualization, F.D.M.-A., A.L.-M., and D.L.V.-M.; methodology, F.D.M.-A., M.N.H., A.L.-M., D.L.V.-M., and M.Á.M.-T.; formal analysis, M.Á.M.-T., F.D.M.-A., and M.N.H.; writing—original draft preparation, M.Á.M.-T.; review and editing, A.L.-M., F.D.M.-A., and D.L.V.-M.; project administration, D.L.V.-M.; funding acquisition, F.D.M.-A. and D.L.V.-M. All authors have read and agreed to the published version of the manuscript.

Funding

This research is part of the project PID2019-111293RB-I00 “Improving profitability in greenhouses by increasing photosynthetic activity with passive climate control techniques (GREENPHOC)” funded by the National R+D+i Plan Project of the Spanish Ministry of Economy and Competitiveness and ERDF funds.

Data Availability Statement

Data is contained within the article.

Acknowledgments

The authors would like to thank POLITIV EUROPE, the University of Almería-ANECOOP Foundation for their collaboration and assistance during the development of this study, and the CIAIMBITAL Research Centre.

Conflicts of Interest

The authors declare no conflicts of interest.

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Figure 1. Scheme 3D of the experimental greenhouse (a) and locations of the plant rows (R1–R3) used to measure growth, production, and photosynthesis parameters (b).
Figure 1. Scheme 3D of the experimental greenhouse (a) and locations of the plant rows (R1–R3) used to measure growth, production, and photosynthesis parameters (b).
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Figure 2. Pepper crop in the East sector with black polypropylene plastic mulch (a) and in the West sector with white polyethylene plastic mulch (b).
Figure 2. Pepper crop in the East sector with black polypropylene plastic mulch (a) and in the West sector with white polyethylene plastic mulch (b).
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Figure 3. Evolution of mean (a) and maximum (b) PAR values recorded outdoors at 5 m height (····) and inside the greenhouse: eastern sector with black polypropylene mulch (––) and western sector with white polyethylene mulch (––). External solar radiation measured at 9 m height (- - -).
Figure 3. Evolution of mean (a) and maximum (b) PAR values recorded outdoors at 5 m height (····) and inside the greenhouse: eastern sector with black polypropylene mulch (––) and western sector with white polyethylene mulch (––). External solar radiation measured at 9 m height (- - -).
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Figure 4. Evolution of daily mean (a), maximum (b), and minimum (c) air temperatures recorded outdoors at 9 m height (- - -) and 5 m height (····), inside of greenhouse, eastern sector with black polypropylene mulch (––), and western sector with white polyethylene mulch (––).
Figure 4. Evolution of daily mean (a), maximum (b), and minimum (c) air temperatures recorded outdoors at 9 m height (- - -) and 5 m height (····), inside of greenhouse, eastern sector with black polypropylene mulch (––), and western sector with white polyethylene mulch (––).
Horticulturae 12 00174 g004aHorticulturae 12 00174 g004b
Figure 5. Evolution of daily mean (a), maximum (b), and minimum (c) relative humidity values recorded at 9 m height (- - -) and 5 m height (····), and inside the greenhouse: eastern sector with black polypropylene mulch (––) and western sector with white polyethylene mulch (––).
Figure 5. Evolution of daily mean (a), maximum (b), and minimum (c) relative humidity values recorded at 9 m height (- - -) and 5 m height (····), and inside the greenhouse: eastern sector with black polypropylene mulch (––) and western sector with white polyethylene mulch (––).
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Figure 6. PAR (a) and photosynthetic activity (b) of pepper crops with white polyethylene (Horticulturae 12 00174 i001) and black polypropylene mulch (Horticulturae 12 00174 i002). Mean value (×) and median (—) with the lines indicating the maximum and minimum values measured (I) and values between the 25th and 75th percentile (▯).
Figure 6. PAR (a) and photosynthetic activity (b) of pepper crops with white polyethylene (Horticulturae 12 00174 i001) and black polypropylene mulch (Horticulturae 12 00174 i002). Mean value (×) and median (—) with the lines indicating the maximum and minimum values measured (I) and values between the 25th and 75th percentile (▯).
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Figure 7. Marketable (and ) and total ( and ) yield of pepper crops in the East sector with black polypropylene mulch and in the West sector with white polyethylene mulch. Error bars represent standard deviation.
Figure 7. Marketable (and ) and total ( and ) yield of pepper crops in the East sector with black polypropylene mulch and in the West sector with white polyethylene mulch. Error bars represent standard deviation.
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Table 1. Characteristics of the two sectors of the experimental greenhouse. Greenhouse soil surface SC (m2), roof vents surface SRV (m2), side vents surface SSV, and ventilation surface/greenhouse surface ratio SV/SC (%).
Table 1. Characteristics of the two sectors of the experimental greenhouse. Greenhouse soil surface SC (m2), roof vents surface SRV (m2), side vents surface SSV, and ventilation surface/greenhouse surface ratio SV/SC (%).
SectorPlastic MulchDimensionsSCSRVSSVSRV+SSV/SC
EastBlack polypropylene 18 m × 25 m45040.50127.2628.3
WestWhite polyethylene18 m × 20 m36031.5070.4028.3
Table 2. Means RPARm and daily maximum RPARMAX, values of photosynthetically active radiation measured at the centre of the western and eastern greenhouse sectors.
Table 2. Means RPARm and daily maximum RPARMAX, values of photosynthetically active radiation measured at the centre of the western and eastern greenhouse sectors.
SectorBlack PolypropyleneWhite Polyethylene
RPARm [μmol·m−2·s−1]183.3190.1
RPARMAX [μmol·m−2·s−1]740.7756.0
Table 3. Means Tm, minimum TMIN, and daily maximum TMAX air temperatures measured in the western (white polyethylene mulch) and eastern (black polypropylene mulch) sectors of the greenhouse during the entire experimental period.
Table 3. Means Tm, minimum TMIN, and daily maximum TMAX air temperatures measured in the western (white polyethylene mulch) and eastern (black polypropylene mulch) sectors of the greenhouse during the entire experimental period.
SectorBlack PolypropyleneWhite Polyethylene
SubsectorNorthSouthNorthSouth
Mean temperature, Tm [°C]21.221.021.721.4
Maximum temperature, TMAX [°C]30.930.831.931.2
Minimum temperature, TMIN [°C]14.013.614.514.2
Table 4. Means Hm, minimum HMIN, and daily maximum HMAX relative humidity values measured in the western (white polyethylene mulch) and eastern (black polypropylene mulch) sectors of the greenhouse during the entire experimental period.
Table 4. Means Hm, minimum HMIN, and daily maximum HMAX relative humidity values measured in the western (white polyethylene mulch) and eastern (black polypropylene mulch) sectors of the greenhouse during the entire experimental period.
SectorBlack PolypropyleneWhite Polyethylene
SubsectorNorthSouthNorthSouth
Mean relative humidity, Hm [%]61.662.161.162.1
Maximum relative humidity, HMAX [%]84.985.684.986.1
Minimum relative humidity, HMIN [%]32.332.631.432.3
Table 5. Average values (±standard deviations) of the measurements made on the leaves of plants grown in the two greenhouse sectors with different plastic mulch. Photosynthetic activity PA [µmol CO2 m−2 s−1], PAR QPAR [µmol m−2 s−1], leaf temperature TL [°C], CO2 concentration CL [ppm], evapotranspiration EL [mmol m−2 s−1], and stomatal conductance CE [mol m−2 s−1].
Table 5. Average values (±standard deviations) of the measurements made on the leaves of plants grown in the two greenhouse sectors with different plastic mulch. Photosynthetic activity PA [µmol CO2 m−2 s−1], PAR QPAR [µmol m−2 s−1], leaf temperature TL [°C], CO2 concentration CL [ppm], evapotranspiration EL [mmol m−2 s−1], and stomatal conductance CE [mol m−2 s−1].
SectorsPlastic MulchPAQPARTLCLELCE
EastBlack polypropylene9.2 a ± 1.1534.8 a ± 54.428.2 a ± 1.4421.9 a ± 5.83.2 a ± 0.7299.1 a ±105.6
WestWhite polyethylene9.8 a ± 2.0554.9 a ± 56.829.8 a ± 1.5420.3 a ± 5.43.4 a ±0.7261.7 a ±104.5
a Values with different letters in the same column show statistically significant differences with a confidence level of 95.0% (p-value ≤ 0.05).
Table 6. Statistical analysis of the growth parameters of the pepper crop (mean values ± standard deviation) in the two sectors of the experimental greenhouse. Plant height (HP) [cm], plant width (PW) [cm], stem diameter (DS) [mm], number of nodes (NN), internodes length (IL) [cm], leaf length (LL) [cm], leaf width (LW) [cm].
Table 6. Statistical analysis of the growth parameters of the pepper crop (mean values ± standard deviation) in the two sectors of the experimental greenhouse. Plant height (HP) [cm], plant width (PW) [cm], stem diameter (DS) [mm], number of nodes (NN), internodes length (IL) [cm], leaf length (LL) [cm], leaf width (LW) [cm].
SectorsPlastic MulchHPWPDSNNILLLLW
EastBlack polypropylene73.2 a ± 23.6 46.6 a ± 12.312.4 a ± 2.610.4 a ± 2.96.4 a ± 1.916.5 a ± 3.19.4 a ± 1.9
WestWhite polyethylene66.9 a ± 21.545.1 a ± 12.312.2 a ± 2.910.1 a ± 2.76.7 a ± 2.315.9 a ± 3.59.0 a ± 1.7
a Values with different letters in the same column show statistically significant differences with a confidence level of 95.0% (p-value ≤ 0.05).
Table 7. Average values (±standard deviations) of the production quality parameters measured for plants grown in the two greenhouse sectors with different plastic mulch. Weight WF [g], length [cm], width WiF [mm], firmness FF [kg cm], soluble solids content SSC [° Brix], and dry matter DM [%].
Table 7. Average values (±standard deviations) of the production quality parameters measured for plants grown in the two greenhouse sectors with different plastic mulch. Weight WF [g], length [cm], width WiF [mm], firmness FF [kg cm], soluble solids content SSC [° Brix], and dry matter DM [%].
SectorsPlastic MulchWFLFWiFFFSSCDM
EastBlack polypropylene173.4 a ± 32.87.8 a ± 1.181.9 a ± 6.32.7 a ± 0.67.8 a ± 1.89.3 a ±1.8
WestWhite polyethylene201.3 b ± 28.48.0 a ± 0.981.0 a ± 9.62.7 a ± 0.96.5 a ±1.48.7 a ±1.7
a,b Values with different letters in the same column show statistically significant differences with a confidence level of 95.0% (p-value ≤ 0.05).
Table 8. Average values (±standard deviations) of the colour characteristics measured in pepper fruits harvested in sectors with different plastic mulch. Colorimetric coordinates corresponding to the luminosity L*, the red/green colour component a*, the yellow/blue colour component b*, and the chromaticity a*/b*.
Table 8. Average values (±standard deviations) of the colour characteristics measured in pepper fruits harvested in sectors with different plastic mulch. Colorimetric coordinates corresponding to the luminosity L*, the red/green colour component a*, the yellow/blue colour component b*, and the chromaticity a*/b*.
SectorsPlastic MulchL*a*b*a*/b*
EastBlack polypropylene33.2 a ± 2.725.7 a ± 5.924.9 a ± 9.91.2 a ± 0.5
WestWhite polyethylene33.0 a ± 2.724.5 a ± 8.526.4 a ± 15.31.1 a ± 0.4
a Values with different letters in the same column show statistically significant differences with a confidence level of 95.0% (p-value ≤ 0.05).
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Moreno-Teruel, M.Á.; Molina-Aiz, F.D.; Honoré, M.N.; López-Martínez, A.; Valera-Martínez, D.L. Effect of Reflective Plastic Mulches on the Microclimate, Photosynthetic Activity, and Yield of Pepper (Capsicum annuum L.) in a Multispan Greenhouse. Horticulturae 2026, 12, 174. https://doi.org/10.3390/horticulturae12020174

AMA Style

Moreno-Teruel MÁ, Molina-Aiz FD, Honoré MN, López-Martínez A, Valera-Martínez DL. Effect of Reflective Plastic Mulches on the Microclimate, Photosynthetic Activity, and Yield of Pepper (Capsicum annuum L.) in a Multispan Greenhouse. Horticulturae. 2026; 12(2):174. https://doi.org/10.3390/horticulturae12020174

Chicago/Turabian Style

Moreno-Teruel, María Ángeles, Francisco Domingo Molina-Aiz, Mireille Nathalie Honoré, Alejandro López-Martínez, and Diego Luis Valera-Martínez. 2026. "Effect of Reflective Plastic Mulches on the Microclimate, Photosynthetic Activity, and Yield of Pepper (Capsicum annuum L.) in a Multispan Greenhouse" Horticulturae 12, no. 2: 174. https://doi.org/10.3390/horticulturae12020174

APA Style

Moreno-Teruel, M. Á., Molina-Aiz, F. D., Honoré, M. N., López-Martínez, A., & Valera-Martínez, D. L. (2026). Effect of Reflective Plastic Mulches on the Microclimate, Photosynthetic Activity, and Yield of Pepper (Capsicum annuum L.) in a Multispan Greenhouse. Horticulturae, 12(2), 174. https://doi.org/10.3390/horticulturae12020174

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