Search Results (16)

Deoxynivalenol (DON) is a common mycotoxin that contaminates cereals. Therefore, the development of sensitive and efficient detection methods for DON is essential to guarantee food safety and human health. In this study, an enzyme cascade amplification-based immunoassay (ECAIA) using a dual-functional alkaline phosphatase-linked single-chain fragment variable fusion tracer (scFv-ALP) and MnO₂ nanosheets was established for DON detection. The scFv-ALP effectively catalyzes the hydrolysis of ascorbyl-2-phosphate (AAP) to produce ascorbic acid (AA). This AA subsequently interacts with MnO₂ nanosheets to initiate a redox reaction that results in the loss of oxidizing properties of MnO₂. In the absence of ALP, MnO₂ nanosheets can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce the blue oxidized product of TMB, which exhibits a signal at a wavelength of 650 nm for quantitative analysis. After optimization, the ECAIA had a limit of detection of 0.45 ng/mL and a linear range of 1.2–35.41 ng/mL. The ECAIA exhibited good accuracy in recovery experiments and high selectivity for DON. Moreover, the detection results of the actual corn samples correlated well with those from high-performance liquid chromatography. Overall, the proposed ECAIA based on the scFv-ALP and MnO₂ nanosheets was demonstrated as a reliable tool for the detection of DON in corn samples. Full article

In this work, the S@g-C₃N₄ and CuS@g-C₃N₄ catalysts were prepared via the polycondensation process. The structural properties of these samples were completed on XRD, FTIR and ESEM techniques. The XRD pattern of S@g-C₃N₄ presents a sharp peak at 27.2° and a weak peak at 13.01° and the reflections of CuS belong to the hexagonal phase. The interplanar distance decreased from 0.328 to 0.319 nm that facilitate charge carrier separation and promoting H₂ generation. FTIR data revealed the structural change according to absorption bands of g-C₃N₄. ESEM images of S@g-C₃N₄ exhibited the described layered sheet structure for g-C₃N₄ materials and CuS@g-C₃N₄ demonstrated that the sheet materials were fragmented throughout the growth process. The data of BET revealed a higher surface area (55 m²/g) for the CuS-g-C₃N₄ nanosheet. The UV–vis absorption spectrum of S@g-C₃N₄ showed a strong peak at 322 nm, which weakened after the growth of CuS at g-C₃N₄. The PL emission data showed a peak at 441 nm, which correlated with electron–hole pair recombination. The data of hydrogen evolution showed improved performance for the CuS@g-C₃N₄ catalyst (5227 mL/g·min). Moreover, the activation energy was determined for S@g-C₃N₄ and CuS@g-C₃N₄, which showed a lowering from 47.33 ± 0.02 to 41.15 ± 0.02 KJ/mol. Full article

β-lactoglobulin (β-Lg) is a protein found in milk that can cause severe allergic reactions, including rash, vomiting, and diarrhea. Thus, it is crucial to develop a sensitive β-Lg detection method to protect people who are susceptible to allergies. Here, we introduce a novel and highly sensitive fluorescent aptamer biosensor for detecting β-Lg. First, a fluorescein-based dye (FAM)-labeled β-lactoglobulin aptamer (β-Lg aptamer) is adsorbed on the surface of tungsten disulfide (WS₂) nanosheets via van der Waals forces, resulting in fluorescence quenching. When β-Lg is present, the β-Lg aptamer selectively binds to β-Lg, causing a conformational change in the β-Lg aptamer and releasing it from the surface of WS₂ nanosheets, which restores the fluorescence signal. Simultaneously, DNase I in the system cleaves the aptamer bound to the target, producing a short oligonucleotide fragment and releasing β-Lg. The released β-Lg then binds to another β-Lg aptamer adsorbed on WS₂, initiating the next round of cleavage, resulting in significant amplification of the fluorescence signal. This method has a linear detection range of 1–100 ng mL⁻¹, and the limit of detection is 0.344 ng mL⁻¹. Furthermore, this approach has been successfully used for detecting β-Lg in milk samples with satisfactory results, providing new opportunities for food analysis and quality control. Full article

The influence of the average surface area of different graphene nanoplatelets (GNP) on the thermo-electrical behaviour, associated with Joule heating, and the attenuation of electromagnetic signals of epoxy composites has been studied, analysing the effect of the morphology obtained as a function of the dispersion time by ultrasonication and the GNP content added. Gravity moulding was used as the first stage in the scaling-up, oriented to the industrial manufacture of multilayer coatings, observing a preferential self-orientation of nanoparticles and, in several conditions, a self-stratification too. The increase of sonication time during the GNP dispersion provides a decrease in the electrical conductivity, due to the GNP fragmentation. Instead, the thermal conductivity is enhanced due to the higher homogeneous distribution of GNPs into the epoxy matrix. Finally, the lower surface area of GNPs reduces the thermal and electrical conductivity due to a greater separation between nanosheets. Regarding the study of the attenuation of electromagnetic waves, it has been discovered that in the frequency range from 100 Hz to 20 MHz, this attenuation is independent of the direction of analysis, the type of graphene, the sonication time, and the state of dispersion of the nano-reinforcement in the matrix. Furthermore, it has also been observed that the conservation of the constant shielding values for the three types of GNPs are in a range of average frequencies between 0.3 and 3 MHz. Full article

In this study, both vanadium and copper elements were anchored on graphitic carbon nitride (gCN) (denoted as V/Cu/gCN) via a thermal decomposition process as a novel nanosheet photocatalyst for the removal of monocrotophos (MCP). The prepared nanosheet features were studied by utilizing XRD, UV–Visible absorption spectrometry, PL, FE-SEM, TEM, and XPS techniques. These analytical techniques revealed the successful formation of direct Z-scheme heterojunctions of V/Cu/gCN nanosheets. The dopant materials significantly enhanced the electron–hole separation and enhanced the removal rate of MCP as compared with bulk gCN. The investigation of effective operating conditions confirmed that a higher removal of MCP could be obtained at a doping concentration of 0.3 wt% and a catalytic dosage of 8 mg with 80 min of visible-light irradiation. The generation of various reactive radicals during the degradation process of the photocatalyst was observed using a scavenging treatment process. Additionally, the scavenging process confirmed that e⁻, OH•, h⁺, and O₂^•− played a major role in MCP degradation. The direct Z-scheme dual-heterojunction mechanism, as well as the possible pathway for the fragmentation of MCP by the V/Cu/gCN nanosheet photocatalyst, was derived in detail. This research article provides a novel perspective on the formation of excellent semiconductor photocatalysts, which exhibit enormous potential for environmental treatments. Full article

Harmful algal blooms (HABs) are common disastrous ecological anomalies in coastal waters. An effective algae monitoring approach is important for natural disaster warning and environmental governance. However, conducting rapid and sensitive detection of multiple algae is still challenging. Here, we designed an ultrasensitive, rapid and portable double-layer microfluidic biochip for the simultaneous quantitative detection of six species of algae. Specific DNA probes based on the 18S ribosomal DNA (18S rDNA) gene fragments of HABs were designed and labeled with the fluorescent molecule cyanine-3 (Cy3). The biochip had multiple graphene oxide (GO) nanosheets-based reaction units, in which GO nanosheets were applied to transfer target DNA to the fluorescence signal through a photoluminescence detection system. The entire detection process of multiple algae was completed within 45 min with the linear range of fluorescence recovery of 0.1 fM–100 nM, and the detection limit reached 108 aM. The proposed approach has a simple detection process and high detection performance and is feasible to conduct accurate detection with matched portable detection equipment. It will have promising applications in marine natural disaster monitoring and environmental care. Full article

Well-defined Zn₂GeO₄/g-C₃N₄ nanocomposites with a band alignment of type-I were prepared by the ultrasound-assisted solvent method, starting from g-C₃N₄ nanosheets and incorporating 0, 10, 20, and 40 wt% of Zn₂GeO₄. In this study, we have investigated in-depth the photoluminescence emission and photocatalytic activity of these nanocomposites. Our experimental results showed that an increased mass ratio of Zn₂GeO₄ to g-C₃N₄ can significantly improve their photoluminescence and photocatalytic responses. Additionally, we have noted that the broadband photoluminescence (PL) emission for these nanocomposites reveals three electronic transitions; the first two well-defined transitions (at ca. 450 nm and 488 nm) can be attributed to π*→ lone pair (LP) and π*→π transitions of g-C₃N₄, while the single shoulder at ca. 532 nm is due to the oxygen vacancy (Vo) as well as the hybridization of 4s and 4p orbital states in the Zn and Ge belonging to Zn₂GeO₄. These experimental findings are also supported by theoretical calculations performed under periodic conditions based on the density functional theory (DFT) fragment. The theoretical findings for these nanocomposites suggest a possible strain-induced increase in the Zn-O bond length, as well as a shortening of the Ge-O bond of both tetrahedral [ZnO₄] and [GeO₄] clusters, respectively. Thus, this disordered structure promotes local polarization and a charge gradient in the Zn₂GeO₄/g-C₃N₄ interface that enable an efficient separation and transfer of the photoexcited charges. Finally, theoretical results show a good correlation with our experimental data. Full article

Metal halide perovskite nanocrystals, an emerging class of materials for advanced photonic and optoelectronic applications, are mainly fabricated with colloidal chemistry routes. On the quest for new properties according to application needs, new perovskite systems of various morphologies and levels of doping and alloying have been developed, often also involving post-synthesis reactions. Recently, laser irradiation in liquids has been utilized as a fast method to synthesize or transform materials and interesting laser-induced transformations on nanocrystals were induced. These studies in general have been limited to small nanocrystals (~15 nm). In the case of halide perovskites, fragmentation or anion exchange have been observed in such laser-based processes, but no crystal structure transformations were actually observed or deliberately studied. Nanocrystals are more sensitive to light exposure compared to the corresponding bulk crystals. Additional factors, such as size, morphology, the presence of impurities, and others, can intricately affect the photon absorption and heat dissipation in nanocrystal suspensions during laser irradiation. All these factors can play an important role in the final morphologies and in the time required for these transformations to unfold. In the present work, we have employed a 513 nm femtosecond (fs) laser to induce different transformations in large nanocrystals, in which two phases coexist in the same particle (Cs₄PbBr₆/CsPbBr₃ nanohexagons of ~100 nm), dispersed in dichlorobenzene. These transformations include: (i) the exfoliation of the primary nanohexagons and partial anion exchange; (ii) fragmentation in smaller nanocubes and partial anion exchange; (iii) side-by-side-oriented attachment, fusion, and formation of nanoplatelets and complete anion exchange; (iv) side-by-side attachment, fusion, and formation of nanosheets. Partial or complete Br-Cl anion exchange in the above transformations was triggered by the partial degradation of dichlorobenzene. In addition to the detailed analysis of the various nanocrystal morphologies observed in the various transformations, the structure–photoluminescence relationships for the different samples were analyzed and discussed. Full article

The noble, metal-free materials capable of efficiently catalyzing water splitting reactions currently hold a great deal of promise. In this study, we reported the structure and electrochemical performance of new MoS₂-based material synthesized with L-cysteine. For this, a facile one-pot hydrothermal process was developed and an array of densely packed nanoplatelet-shaped hybrid species directly on a conductive substrate were obtained. The crucial role of L-cysteine was determined by numerous methods on the structure and composition of the synthesized material and its activity and stability for hydrogen evolution reaction (HER) from the acidic water. A low Tafel slope of 32.6 mV dec⁻¹, close to a Pt cathode, was registered for the first time. The unique HER performance at the surface of this hybrid material in comparison with recently reported MoS₂-based electrocatalysts was attributed to the formation of more defective 1T, 2H-MoS₂/MoO_x, C nanostructures with the dominant 1T-MoS₂ phase and thermally degraded cysteine residues entrapped. Numerous stacks of metallic (1T-MoS₂ and MoO₂) and semiconducting (2H-MoS₂ and MoO₃) fragments relayed the formation of highly active layered nanosheets possessing a low hydrogen adsorption free energy and much greater durability, whereas intercalated cysteine fragments had a low Tafel slope of the HER reaction. X-ray photoelectron spectroscopy, scanning electron microscopy, thermography with mass spectrometry, high-resolution transmission electron microscopy, Raman spectroscopy techniques, and linear sweep voltammetry were applied to verify our findings. Full article

Synthetic organic 2D materials are attracting careful attention of researchers due to their excellent functionality in various applications, including storage batteries, catalysis, thermoelectricity, advanced electronics, superconductors, optoelectronics, etc. In this work, thiacalix[4]arene derivatives functionalized by geranyl fragments at the lower rim in cone and 1,3-alternate conformations, that are capable of controlled self-assembly in a 2D nanostructures were synthesized. X-ray diffraction analysis showed the formation of 2D monomolecular-layer nanosheets from synthesized thiacalix[4]arenes, the distance between which depends on the stereoisomer used. It was established by DSC, FSC, and PXRD methods that the obtained macrocycles are capable of forming different crystalline polymorphs, moreover dimethyl sulphoxide (DMSO) is contributing to the formation of a more stable polymorph for cone stereoisomer. The obtained crystalline 2D materials based on synthesized thiacalix[4]arenes can find application in material science and medicine for the development of modern pharmaceuticals and new generation materials. Full article

Graphene shows great potential applications in functional coating, electrodes, and ultrasensitive sensors, but high-yield and scalable preparation of few-layer graphene (FLG) by mechanical exfoliation method is still a formidable challenge. In this work, a novel two-step method for high-yield preparation of FLG is developed by combining resonance ball milling and hydrothermal treatment. During the resonance ball milling process, the utilization of magnetic Fe₃O₄ nanoparticles as a new “particle wedge” is beneficial to facilitate fragment and delamination of graphitic layers. In addition, further hydrothermal treatment can enhance ball milling product (BMP) exfoliation because of the shear force driven by the Brownian motion of various molecules at high temperature and high pressure. As expected, the two-step method can have high exfoliation efficiency up to 92% (≤10 layers). Moreover, the FLG nanosheet ink can easily achieve the formation of FLG coatings on the surface of various substrates, resulting in good electrical conductivity, which possesses potential applications in various fields including functional coating, energy storages, and electrochemical sensors, etc. Our work provides a new-fashioned strategy for mechanical large-scale production of graphene. Full article

This work launches the first-ever report on the fabrication of waterborne epoxy-graphene oxide (GO) coatings (WEGC) using a block polymer as a dispersant of GO, wherein the block polymer was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization of acrylic acid and oligo(ethylene glycol) methyl ether methacrylate A number of analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), thermo gravimetric analysis (TGA), and salt spray tests, were utilized to explore the morphology and performance of the WEGC. It was confirmed that POEGMA950-b-PAA attached to the GO nanosheets, increasing the integral space of the sheets. Modified GO (MGO) layers were well-dispersed in the epoxy matrix through the formation of a GO-dispersant-epoxy ternary molecular structure. Furthermore, the presence of MGO substantially influenced the thermal properties, mechanical properties, and anticorrosion performance of the WEGC. TGA, salt spray tests, and pull-off testsshowed that 0.5 wt.% MGO content achieved the greatest improvement in the evaluated properties. Full article

Cobalt-doped graphene-coupled hypercrosslinked polymers (Co-GHCP) have been successfully prepared on a large scale, using an efficient RAFT (Reversible Addition-Fragmentation Chain Transfer Polymerization) emulsion polymerization and nucleophilic substitution reaction with Co (II) porphyrin. The Co-GHCP could be transformed into cobalt-doped porous carbon nanosheets (Co-GPC) through direct pyrolysis treatment. Such a Co-GPC possesses a typical 2D morphology with a high specific surface area of 257.8 m² g⁻¹. These intriguing properties of transition metal-doping, high conductivity, and porous structure endow the Co-GPC with great potential applications in energy storage and conversion. Utilized as an electrode material in a supercapacitor, the Co-GPC exhibited a high electrochemical capacitance of 455 F g⁻¹ at a specific current of 0.5 A g⁻¹. After 2000 charge/discharge cycles, at a current density of 1 A g⁻¹, the specific capacitance increased by almost 6.45%, indicating the excellent capacitance and durability of Co-GPC. These results demonstrated that incorporation of metal porphyrin into the framework of a hypercrosslinked polymer is a facile strategy to prepare transition metal-doped porous carbon for energy storage applications. Full article

The fabrication technique of ultrathin film (commonly known as nanosheets) has been significantly developed over the years. Due to the mechanical properties of nanosheets, such as high levels of adhesion and flexibility, this made nanosheets the ideal candidate in biomedical applications. In this review, innovative biomedical applications of nanosheets are discussed, which include, drug delivery, wound treatment, and functional nanosheets towards flexible biodevices, etc. Finally, the future outlook of nanosheet technology towards a biomedical application is discussed. Full article

We developed a new method for detecting S1 nuclease and hydroxyl radicals based on the use of water-soluble conjugated poly[9,9-bis(6,6-(N,N,N-trimethylammonium)-fluorene)-2,7-ylenevinylene-co-alt-2,5-dicyano-1,4-phenylene)] (PFVCN) and tungsten disulfide (WS₂) nanosheets_. Cationic PFVCN is used as a signal reporter, and single-layer WS₂ is used as a quencher with a negatively charged surface. The ssDNA forms complexes with PFVCN due to much stronger electrostatic interactions between cationic PFVCN and anionic ssDNA, whereas PFVCN emits yellow fluorescence. When ssDNA is hydrolyzed by S1 nuclease or hydroxyl radicals into small fragments, the interactions between the fragmented DNA and PFVCN become weaker, resulting in PFVCN being adsorbed on the surface of WS₂ and the fluorescence being quenched through fluorescence resonance energy transfer. The new method based on PFVCN and WS₂ can sense S1 nuclease with a low detection limit of 5 × 10⁻⁶ U/mL. Additionally, this method is cost-effective by using affordable WS₂ as an energy acceptor without the need for dye-labeled ssDNA. Furthermore, the method provides a new platform for the nuclease assay and reactive oxygen species, and provides promising applications for drug screening. Full article